                         SEQUENCE LISTING

<110>  Alida Biosciences, Inc.
	Jerome Santos
	Yu-Hsien Hwang-Fu
 
<120>  MULTIPLEXED PROFILING OF RNA AND DNA MODIFICATIONS

<130>  ALID-001/02WO 342094-2004

<150>  US 63/193,402
<151>  2021-05-26

<150>  US 63/118,409
<151>  2020-11-25

<160>  60    

<170>  PatentIn version 3.5

<210>  1
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  biotinylated

<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  n is any ribonucleotide

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  N6-methyladenosine

<220>
<221>  misc_feature
<222>  (10)..(17)
<223>  n is any ribonucleotide

<400>  1
nnnnnnnnnn nnnnnnn                                                      17


<210>  2
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  biotinylated

<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  n is any ribonucleotide

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  inosine

<220>
<221>  misc_feature
<222>  (10)..(17)
<223>  n is any ribonucleotide

<400>  2
nnnnnnnnnn nnnnnnn                                                      17


<210>  3
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  biotinylated

<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  n is any ribonucleotide

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  pseudouridine

<220>
<221>  misc_feature
<222>  (10)..(17)
<223>  n is any ribonucleotide

<400>  3
nnnnnnnnnn nnnnnnn                                                      17


<210>  4
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  biotinylated

<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  n is any ribonucleotide

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  5-methylcytosine

<220>
<221>  misc_feature
<222>  (10)..(17)
<223>  n is any ribonucleotide

<400>  4
nnnnnnnnnn nnnnnnn                                                      17


<210>  5
<211>  16
<212>  RNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  biotinylated

<220>
<221>  misc_feature
<222>  (1)..(16)
<223>  n is any ribonucleotide

<400>  5
nnnnnnnnnn nnnnnn                                                       16


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' preadenylation

<220>
<221>  modified_base
<222>  (21)..(21)
<223>  dideoxycytidine

<400>  6
agatcggaag agcggttcag n                                                 21


<210>  7
<211>  4
<212>  DNA
<213>  Unknown

<220>  
<223>  BamHI Restriction site

<400>  7
gatc                                                                     4


<210>  8
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

 
<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Phosphate

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  n is any nucleotide

<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  n is any nucleotide

<400>  8
nnaaccnnna gatcggaaga gcgtcgtgga tcctgaaccg c                           41


<210>  9
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' amine

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  PEG linker

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  blocked 3' end

<400>  9
ttataagaga cagacacagg ccactcagtc tat                                    33


<210>  10
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  10

Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
1               5                   10              


<210>  11
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa is any amino acid

<400>  11

Leu Cys Xaa Pro Xaa Arg 
1               5       


<210>  12
<211>  139
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  12

Met Ser Tyr Tyr His His His His His His Asp Tyr Asp Ile Pro Thr 
1               5                   10                  15      


Thr Glu Asn Leu Tyr Phe Gln Gly Ala Met Val Asp Thr Leu Ser Gly 
            20                  25                  30          


Leu Ser Ser Glu Gln Gly Gln Ser Gly Asp Met Thr Ile Glu Glu Asp 
        35                  40                  45              


Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu 
    50                  55                  60                  


Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile 
65                  70                  75                  80  


Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro 
                85                  90                  95      


Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val 
            100                 105                 110         


Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val 
        115                 120                 125             


Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile 
    130                 135                 


<210>  13
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM labelled

<220>
<221>  misc_feature
<222>  (1)..(42)
<223>  ribonucleotides

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  N6-methyladenosine

<400>  13
ucgucggcag cgucagaugc auaaggucna uauuaaguau agactgagtg                  50


<210>  14
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM labelled

<220>
<221>  misc_feature
<222>  (1)..(32)
<223>  ribonucleotides

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  5-methylcytosine

<400>  14
ucgucggcag cgucagauga uauungaagu auactgagtg                             40


<210>  15
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM labelled

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  5-methylcytosine

<400>  15
tcgtcggcag cgtcagatgc ataatctana tcttaagtat agactgagtg                  50


<210>  16
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM labelled

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  inosine

<400>  16
tcgtcggcag cgtcagatga tactngcagt atactgagtg                             40


<210>  17
<211>  44
<212>  RNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM labelled

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  N6-methyladenosine

<400>  17
ucgucggcag cgucagaugc auaaggucna uauuaaguau agac                        44


<210>  18
<211>  34
<212>  RNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM labelled

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  5-methylcytosine

<400>  18
cgucggcagc gucagaugau auungaagua ugac                                   34


<210>  19
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  19
actgagtg                                                                 8


<210>  20
<211>  25
<212>  DNA
<213>  Artificial Sequence


<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  3' amine

<220>  
<223>  synthetic construct

<400>  20
catctgacgc tgccgacgat ttttt                                             25


<210>  21
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  21
tcgtcggcag cgtcagatga ttgtgttagg ctagtaagta gatggattag accgtcgagt       60

gagtagagta cgtagtgca                                                    79


<210>  22
<211>  33
<212>  DNA
<213>  Artificial Sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Dibenzocyclooctyl labelled, 5' amine

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  PEG linker

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  blocked 3' end

<220>  
<223>  synthetic construct

<400>  22
ttataagaga cagacacagg ccactcagtc tat                                    33


<210>  23
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM labelled

<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  n is any ribonucleotide

<400>  23
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn                                        30


<210>  24
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM labelled

<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  ribonucleotide

<400>  24
uuaaguauag actgagtg                                                     18


<210>  25
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' amine

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  PEG linker

<400>  25
ttataagaga cagacacagg ccactcagt                                         29


<210>  26
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' amine

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  PEG linker

<400>  26
ttataagaga cagacacagg ccactcagtc t                                      31


<210>  27
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' amine

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  PEG linker

<400>  27
ttataagaga cagacacagg ccactcagtc tat                                    33


<210>  28
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Phosphate

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  n is any nucleotide

<220>
<221>  modified_base
<222>  (10)..(10)
<223>  dideoxycytidine

<400>  28
nnactgagtn                                                              10


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Phosphate

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  n is any nucleotide

<220>
<221>  modified_base
<222>  (20)..(20)
<223>  dideoxycytidine

<400>  29
nnactgagtg gcctgtgtcn                                                   20


<210>  30
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Phosphate

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  n is any nucleotide

<220>
<221>  modified_base
<222>  (30)..(30)
<223>  dideoxycytidine

<400>  30
nnactgagtg gcctgtgtct gtctcttatn                                        30


<210>  31
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Phosphate

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  n is any nucleotide

<220>
<221>  modified_base
<222>  (52)..(52)
<223>  dideoxycytidine

<400>  31
nnactgagtg gcctgtgtct gtctcttata cacatctccg agcccacgag an               52


<210>  32
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' FAM labelled

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  N6-methyladenosine

<400>  32
ucgucggcag cgucagaugc cugcaaaggn cugcuuugac                             40


<210>  33
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Phosphate

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  ribonucleotide

<220>
<221>  misc_feature
<222>  (28)..(29)
<223>  PEG linker

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  3' Biotin with triethylene glycol

<400>  33
cggacacaga cagagaatat uatatatat                                         29


<210>  34
<211>  442
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  34

Met Ser Ser Glu Thr Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg 
1               5                   10                  15      


Arg Ile Glu Pro His Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu 
            20                  25                  30          


Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile Asn Trp Gly Gly Arg His 
        35                  40                  45              


Ser Ile Trp Arg His Thr Ser Gln Asn Thr Asn Lys His Val Glu Val 
    50                  55                  60                  


Asn Phe Ile Glu Lys Phe Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr 
65                  70                  75                  80  


Arg Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys 
                85                  90                  95      


Ser Arg Ala Ile Thr Glu Phe Leu Ser Arg Tyr Pro His Val Thr Leu 
            100                 105                 110         


Phe Ile Tyr Ile Ala Arg Leu Tyr His His Ala Asp Pro Arg Asn Arg 
        115                 120                 125             


Gln Gly Leu Arg Asp Leu Ile Ser Ser Gly Val Thr Ile Gln Ile Met 
    130                 135                 140                 


Thr Glu Gln Glu Ser Gly Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser 
145                 150                 155                 160 


Pro Ser Asn Glu Ala His Trp Pro Arg Tyr Pro His Leu Trp Val Arg 
                165                 170                 175     


Leu Tyr Val Leu Glu Leu Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys 
            180                 185                 190         


Leu Asn Ile Leu Arg Arg Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile 
        195                 200                 205             


Ala Leu Gln Ser Cys His Tyr Gln Arg Leu Pro Pro His Ile Leu Trp 
    210                 215                 220                 


Ala Thr Gly Leu Lys Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
225                 230                 235                 240 


Ser Pro His Pro Val Leu Glu Lys Leu Arg Ser Ile Asn Asn Tyr Asn 
                245                 250                 255     


Pro Lys Asp Phe Asp Trp Asn Leu Lys His Gly Arg Val Phe Ile Ile 
            260                 265                 270         


Lys Ser Tyr Ser Glu Asp Asp Ile His Arg Ser Ile Lys Tyr Asn Ile 
        275                 280                 285             


Trp Cys Ser Thr Glu His Gly Asn Lys Arg Leu Asp Ala Ala Tyr Arg 
    290                 295                 300                 


Ser Met Asn Gly Lys Gly Pro Val Tyr Leu Leu Phe Ser Val Asn Gly 
305                 310                 315                 320 


Ser Gly His Phe Cys Gly Val Ala Glu Met Lys Ser Ala Val Asp Tyr 
                325                 330                 335     


Asn Thr Cys Ala Gly Val Trp Ser Gln Asp Lys Trp Lys Gly Arg Phe 
            340                 345                 350         


Asp Val Arg Trp Ile Phe Val Lys Asp Val Pro Asn Ser Gln Leu Arg 
        355                 360                 365             


His Ile Arg Leu Glu Asn Asn Glu Asn Lys Pro Val Thr Asn Ser Arg 
    370                 375                 380                 


Asp Thr Gln Glu Val Pro Leu Glu Lys Ala Lys Gln Val Leu Lys Ile 
385                 390                 395                 400 


Ile Ala Ser Tyr Lys His Thr Thr Ser Ile Phe Asp Asp Phe Ser His 
                405                 410                 415     


Tyr Glu Lys Arg Gln Glu Glu Glu Glu Ser Val Lys Lys Glu Arg Gln 
            420                 425                 430         


Gly Arg Gly Lys His His His His His His 
        435                 440         


<210>  35
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  35

Met Ser Ser Glu Thr Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg 
1               5                   10                  15      


Arg Ile Glu Pro His Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu 
            20                  25                  30          


Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile Asn Trp Gly Gly Arg His 
        35                  40                  45              


Ser Ile Trp Arg His Thr Ser Gln Asn Thr Asn Lys His Val Glu Val 
    50                  55                  60                  


Asn Phe Ile Glu Lys Phe Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr 
65                  70                  75                  80  


Arg Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys 
                85                  90                  95      


Ser Arg Ala Ile Thr Glu Phe Leu Ser Arg Tyr Pro His Val Thr Leu 
            100                 105                 110         


Phe Ile Tyr Ile Ala Arg Leu Tyr His His Ala Asp Pro Arg Asn Arg 
        115                 120                 125             


Gln Gly Leu Arg Asp Leu Ile Ser Ser Gly Val Thr Ile Gln Ile Met 
    130                 135                 140                 


Thr Glu Gln Glu Ser Gly Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser 
145                 150                 155                 160 


Pro Ser Asn Glu Ala His Trp Pro Arg Tyr Pro His Leu Trp Val Arg 
                165                 170                 175     


Leu Tyr Val Leu Glu Leu Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys 
            180                 185                 190         


Leu Asn Ile Leu Arg Arg Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile 
        195                 200                 205             


Ala Leu Gln Ser Cys His Tyr Gln Arg Leu Pro Pro His Ile Leu Trp 
    210                 215                 220                 


Ala Thr Gly Leu Lys Gly Gly Ser Arg Gly Val Pro His Ile Val Met 
225                 230                 235                 240 


Val Asp Ala Tyr Lys Arg Tyr Lys His His His His His His 
                245                 250                 


<210>  36
<211>  320
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  36

Met Val Thr Thr Leu Ser Gly Leu Ser Gly Glu Gln Gly Pro Ser Gly 
1               5                   10                  15      


Asp Met Thr Thr Glu Glu Asp Ser Ala Thr His Ile Lys Phe Ser Lys 
            20                  25                  30          


Arg Asp Glu Asp Gly Arg Glu Leu Ala Gly Ala Thr Met Glu Leu Arg 
        35                  40                  45              


Asp Ser Ser Gly Lys Thr Ile Ser Thr Trp Ile Ser Asp Gly His Val 
    50                  55                  60                  


Lys Asp Phe Tyr Leu Tyr Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala 
65                  70                  75                  80  


Ala Pro Asp Gly Tyr Glu Val Ala Thr Pro Ile Glu Phe Thr Val Asn 
                85                  90                  95      


Glu Asp Gly Gln Val Thr Val Asp Gly Glu Ala Thr Glu Gly Asp Ala 
            100                 105                 110         


His Thr Gly Gly Gly Gly Ser Pro His Pro Val Leu Glu Lys Leu Arg 
        115                 120                 125             


Ser Ile Asn Asn Tyr Asn Pro Lys Asp Phe Asp Trp Asn Leu Lys His 
    130                 135                 140                 


Gly Arg Val Phe Ile Ile Lys Ser Tyr Ser Glu Asp Asp Ile His Arg 
145                 150                 155                 160 


Ser Ile Lys Tyr Asn Ile Trp Cys Ser Thr Glu His Gly Asn Lys Arg 
                165                 170                 175     


Leu Asp Ala Ala Tyr Arg Ser Met Asn Gly Lys Gly Pro Val Tyr Leu 
            180                 185                 190         


Leu Phe Ser Val Asn Gly Ser Gly His Phe Cys Gly Val Ala Glu Met 
        195                 200                 205             


Lys Ser Ala Val Asp Tyr Asn Thr Cys Ala Gly Val Trp Ser Gln Asp 
    210                 215                 220                 


Lys Trp Lys Gly Arg Phe Asp Val Arg Trp Ile Phe Val Lys Asp Val 
225                 230                 235                 240 


Pro Asn Ser Gln Leu Arg His Ile Arg Leu Glu Asn Asn Glu Asn Lys 
                245                 250                 255     


Pro Val Thr Asn Ser Arg Asp Thr Gln Glu Val Pro Leu Glu Lys Ala 
            260                 265                 270         


Lys Gln Val Leu Lys Ile Ile Ala Ser Tyr Lys His Thr Thr Ser Ile 
        275                 280                 285             


Phe Asp Asp Phe Ser His Tyr Glu Lys Arg Gln Glu Glu Glu Glu Ser 
    290                 295                 300                 


Val Lys Lys Glu Arg Gln Gly Arg Gly Lys His His His His His His 
305                 310                 315                 320 


<210>  37
<211>  776
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  37

Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys 
1               5                   10                  15      


Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr 
            20                  25                  30          


Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe 
        35                  40                  45              


Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala 
    50                  55                  60                  


His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile 
65                  70                  75                  80  


Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp 
                85                  90                  95      


Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu 
            100                 105                 110         


Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys 
        115                 120                 125             


Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly 
    130                 135                 140                 


Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 
145                 150                 155                 160 


Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys 
                165                 170                 175     


Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly 
            180                 185                 190         


Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp 
        195                 200                 205             


Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala 
    210                 215                 220                 


Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 
225                 230                 235                 240 


Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser 
                245                 250                 255     


Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro 
            260                 265                 270         


Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp 
        275                 280                 285             


Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala 
    290                 295                 300                 


Leu Lys Ser Tyr Glu Glu Glu Leu Val Lys Asp Pro Arg Ile Ala Ala 
305                 310                 315                 320 


Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln 
                325                 330                 335     


Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala 
            340                 345                 350         


Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn 
        355                 360                 365             


Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile 
    370                 375                 380                 


Glu Gly Arg Ile Ser His Met Gly Ser Gly Ser Ser Gly Ser Gly Glu 
385                 390                 395                 400 


Asn Leu Tyr Phe Gln Gly Met Ser Ser Glu Thr Gly Pro Val Ala Val 
                405                 410                 415     


Asp Pro Thr Leu Arg Arg Arg Ile Glu Pro His Glu Phe Glu Val Phe 
            420                 425                 430         


Phe Asp Pro Arg Glu Leu Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile 
        435                 440                 445             


Asn Trp Gly Gly Arg His Ser Ile Trp Arg His Thr Ser Gln Asn Thr 
    450                 455                 460                 


Asn Lys His Val Glu Val Asn Phe Ile Glu Lys Phe Thr Thr Glu Arg 
465                 470                 475                 480 


Tyr Phe Cys Pro Asn Thr Arg Cys Ser Ile Thr Trp Phe Leu Ser Trp 
                485                 490                 495     


Ser Pro Cys Gly Glu Cys Ser Arg Ala Ile Thr Glu Phe Leu Ser Arg 
            500                 505                 510         


Tyr Pro His Val Thr Leu Phe Ile Tyr Ile Ala Arg Leu Tyr His His 
        515                 520                 525             


Ala Asp Pro Arg Asn Arg Gln Gly Leu Arg Asp Leu Ile Ser Ser Gly 
    530                 535                 540                 


Val Thr Ile Gln Ile Met Thr Glu Gln Glu Ser Gly Tyr Cys Trp Arg 
545                 550                 555                 560 


Asn Phe Val Asn Tyr Ser Pro Ser Asn Glu Ala His Trp Pro Arg Tyr 
                565                 570                 575     


Pro His Leu Trp Val Arg Leu Tyr Val Leu Glu Leu Tyr Cys Ile Ile 
            580                 585                 590         


Leu Gly Leu Pro Pro Cys Leu Asn Ile Leu Arg Arg Lys Gln Pro Gln 
        595                 600                 605             


Leu Thr Phe Phe Thr Ile Ala Leu Gln Ser Cys His Tyr Gln Arg Leu 
    610                 615                 620                 


Pro Pro His Ile Leu Trp Ala Thr Gly Leu Lys Ser Gly Ser Glu Thr 
625                 630                 635                 640 


Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Met Val Thr Thr Leu 
                645                 650                 655     


Ser Gly Leu Ser Gly Glu Gln Gly Pro Ser Gly Asp Met Thr Thr Glu 
            660                 665                 670         


Glu Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly 
        675                 680                 685             


Arg Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys 
    690                 695                 700                 


Thr Ile Ser Thr Trp Ile Ser Asp Gly His Val Lys Asp Phe Tyr Leu 
705                 710                 715                 720 


Tyr Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr 
                725                 730                 735     


Glu Val Ala Thr Pro Ile Glu Phe Thr Val Asn Glu Asp Gly Gln Val 
            740                 745                 750         


Thr Val Asp Gly Glu Ala Thr Glu Gly Asp Ala His Thr Gly Ser Ser 
        755                 760                 765             


Gly Ser His His His His His His 
    770                 775     


<210>  38
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Phosphate

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  PEG linker

<400>  38
ttataagaga cagacacagg ccactcagtc tatacttaa                              39


<210>  39
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Phosphate

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  ribonucleotide

<220>
<221>  misc_feature
<222>  (28)..(29)
<223>  PEG linker

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  3' amine

<400>  39
cggacacaga cagagaatat uatatatat                                         29


<210>  40
<211>  45
<212>  RNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' FAM labelled

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  biotinylated

<400>  40
ggcagcguca gaugcaucau cauaaggucu auauuaagua uagac                       45


<210>  41
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' FAM labelled

<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  ribonucleotide

<220>
<221>  modified_base
<222>  (25)..(25)
<223>  inosine

<400>  41
ucgucggcag cgucagaugc auacnaccca uagactgagt g                           41


<210>  42
<211>  359
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  42

Met Ser Ser Glu Thr Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg 
1               5                   10                  15      


Arg Ile Glu Pro His Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu 
            20                  25                  30          


Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile Asn Trp Gly Gly Arg His 
        35                  40                  45              


Ser Ile Trp Arg His Thr Ser Gln Asn Thr Asn Lys His Val Glu Val 
    50                  55                  60                  


Asn Phe Ile Glu Lys Phe Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr 
65                  70                  75                  80  


Arg Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys 
                85                  90                  95      


Ser Arg Ala Ile Thr Glu Phe Leu Ser Arg Tyr Pro His Val Thr Leu 
            100                 105                 110         


Phe Ile Tyr Ile Ala Arg Leu Tyr His His Ala Asp Pro Arg Asn Arg 
        115                 120                 125             


Gln Gly Leu Arg Asp Leu Ile Ser Ser Gly Val Thr Ile Gln Ile Met 
    130                 135                 140                 


Thr Glu Gln Glu Ser Gly Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser 
145                 150                 155                 160 


Pro Ser Asn Glu Ala His Trp Pro Arg Tyr Pro His Leu Trp Val Arg 
                165                 170                 175     


Leu Tyr Val Leu Glu Leu Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys 
            180                 185                 190         


Leu Asn Ile Leu Arg Arg Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile 
        195                 200                 205             


Ala Leu Gln Ser Cys His Tyr Gln Arg Leu Pro Pro His Ile Leu Trp 
    210                 215                 220                 


Ala Thr Gly Leu Lys Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser 
225                 230                 235                 240 


Ala Thr Pro Glu Ser Met Val Thr Thr Leu Ser Gly Leu Ser Gly Glu 
                245                 250                 255     


Gln Gly Pro Ser Gly Asp Met Thr Thr Glu Glu Asp Ser Ala Thr His 
            260                 265                 270         


Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Arg Glu Leu Ala Gly Ala 
        275                 280                 285             


Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser Thr Trp Ile 
    290                 295                 300                 


Ser Asp Gly His Val Lys Asp Phe Tyr Leu Tyr Pro Gly Lys Tyr Thr 
305                 310                 315                 320 


Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala Thr Pro Ile 
                325                 330                 335     


Glu Phe Thr Val Asn Glu Asp Gly Gln Val Thr Val Asp Gly Glu Ala 
            340                 345                 350         


Thr Glu Gly Asp Ala His Thr 
        355                 


<210>  43
<211>  329
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  43

Met Glu Ala Ser Pro Ala Ser Gly Pro Arg His Leu Met Asp Pro His 
1               5                   10                  15      


Ile Phe Thr Ser Asn Phe Asn Asn Gly Ile Gly Arg His Lys Thr Tyr 
            20                  25                  30          


Leu Cys Tyr Glu Val Glu Arg Leu Asp Asn Gly Thr Ser Val Lys Met 
        35                  40                  45              


Asp Gln His Arg Gly Phe Leu His Asn Gln Ala Lys Asn Leu Leu Cys 
    50                  55                  60                  


Gly Phe Tyr Gly Arg His Ala Glu Leu Arg Phe Leu Asp Leu Val Pro 
65                  70                  75                  80  


Ser Leu Gln Leu Asp Pro Ala Gln Ile Tyr Arg Val Thr Trp Phe Ile 
                85                  90                  95      


Ser Trp Ser Pro Cys Phe Ser Trp Gly Cys Ala Gly Gln Val Arg Ala 
            100                 105                 110         


Phe Leu Gln Glu Asn Thr His Val Arg Leu Arg Ile Phe Ala Ala Arg 
        115                 120                 125             


Ile Tyr Asp Tyr Asp Pro Leu Tyr Lys Glu Ala Leu Gln Met Leu Arg 
    130                 135                 140                 


Asp Ala Gly Ala Gln Val Ser Ile Met Thr Tyr Asp Glu Phe Lys His 
145                 150                 155                 160 


Cys Trp Asp Thr Phe Val Asp His Gln Gly Cys Pro Phe Gln Pro Trp 
                165                 170                 175     


Asp Gly Leu Asp Glu His Ser Gln Ala Leu Ser Gly Arg Leu Arg Ala 
            180                 185                 190         


Ile Leu Gln Asn Gln Gly Asn Ser Gly Ser Glu Thr Pro Gly Thr Ser 
        195                 200                 205             


Glu Ser Ala Thr Pro Glu Ser Met Val Thr Thr Leu Ser Gly Leu Ser 
    210                 215                 220                 


Gly Glu Gln Gly Pro Ser Gly Asp Met Thr Thr Glu Glu Asp Ser Ala 
225                 230                 235                 240 


Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Arg Glu Leu Ala 
                245                 250                 255     


Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser Thr 
            260                 265                 270         


Trp Ile Ser Asp Gly His Val Lys Asp Phe Tyr Leu Tyr Pro Gly Lys 
        275                 280                 285             


Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala Thr 
    290                 295                 300                 


Pro Ile Glu Phe Thr Val Asn Glu Asp Gly Gln Val Thr Val Asp Gly 
305                 310                 315                 320 


Glu Ala Thr Glu Gly Asp Ala His Thr 
                325                 


<210>  44
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  ribonucleotide

<400>  44
agaacagaac agaauagatg ataggaagga tgaaggtgag t                           41


<210>  45
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  45
agaagagaag agaacagatg ataggaagga tgaaggtgag t                           41


<210>  46
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  46
agaacagaac agaauagaug auaggaagga ugaaggugag u                           41


<210>  47
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  47
agaacagaac agaacagaug auaggaagga ugaaggugag u                           41


<210>  48
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  48
gaacagaaca gaacagaaca gaacagaaca gaacagatga taggaaggat gaaggtgagt       60


<210>  49
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<220>
<221>  misc_feature
<222>  (60)..(60)
<223>  SpyTag peptide attached

<400>  49
gaacagaaca gaacagaaca gaacagaaca gaacagatga taggaaggat gaaggtgagt       60


<210>  50
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<220>
<221>  misc_feature
<222>  (38)..(38)
<223>  SpyTag peptide attached

<400>  50
gaacagaaca gaacagaaca gaacagaaca gaacagatga taggaaggat gaaggtgagt       60


<210>  51
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (25)..(52)
<223>  3' FAM labelled

<400>  51
ctactatcct tcctacttcc actca                                             25


<210>  52
<211>  4
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  52
atcg                                                                     4


<210>  53
<211>  4
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  53
aatc                                                                     4


<210>  54
<211>  4
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  54
ttag                                                                     4


<210>  55
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct

<400>  55
gatgatgt                                                                 8


<210>  56
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' amine

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  PEG linker

<220>
<221>  misc_feature
<222>  (23)..(25)
<223>  n is any nucleotide

<220>
<221>  misc_feature
<222>  (40)..(40)
<223>  blocked 3' end

<400>  56
tagacgtgtg ctcttccgat ctnnnactaa ttcactcagt                             40


<210>  57
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' phosphate

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  n is any nucleotide

<220>
<221>  misc_feature
<222>  (31)..(32)
<223>  PEG linker

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  3' amine

<400>  57
actaattnnn agatcggaag agcacacgtc tt                                     32


<210>  58
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  58
ctgtctctta tacacatct                                                    19


<210>  59
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  59
agatgtgtat aagagacag                                                    19


<210>  60
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct


<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  ribonucleotide

<220>
<221>  misc_feature
<222>  (29)..(40)
<223>  n is any nucleotide

<400>  60
tttgtgaugc gatgaactca gagtgcttnn nnnnnnnnnn agatgtgtat aagagacag        59


