                         SEQUENCE LISTING

<110>  ImmVira Co., Limited
 
<120>  ONCOLYTIC HERPES SIMPLEX TYPE 1 VIRUSES FOR TREATMENT OF BRAIN 
       TUMORS

<130>  191781-21D-WOP

<150>  PCT/CN2020/133943
<151>  2020-12-4

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  121
<212>  PRT
<213>  Homo sapiens


<220>
<223>  anti-hPD-1 Fab heavy chain variable region

<400>  1

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Leu Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser Gly Gly Gly Gly Asp Thr Tyr Phe Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Val Arg Phe Gly Gly Ala Gly Tyr Tyr Trp Tyr Phe Asp Val Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120     


<210>  2
<211>  330
<212>  PRT
<213>  Homo sapiens


<220>
<223>  anti-hPD-1 Fab heavy chain constant region

<400>  2

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  3
<211>  111
<212>  PRT
<213>  Homo sapiens


<220>
<223>  anti-hPD-1 Fab light chain variable region

<400>  3

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Lys Ser Val Asp Asp Ser 
            20                  25                  30          


Gly Ile Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro 
        35                  40                  45              


Arg Leu Leu Ile Tyr Ala Ala Ser Asn Gln Gly Ser Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys His Gln Thr Lys 
                85                  90                  95      


Glu Val Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  4
<211>  107
<212>  PRT
<213>  Homo sapiens


<220>
<223>  anti-hPD-1 Fab light chain constant region

<400>  4

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  5
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
gaagatctaa tatttttatt gcaactccct g                                      31


<210>  6
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
ctagctagct tataaaaggc gcgtcccgtg g                                      31


<210>  7
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
gctctagatt gcgacgcccc ggctc                                             25


<210>  8
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
ccttaattaa ggttaccacc ctgtagcccc gatgt                                  35


<210>  9
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  9
tcccatggat ttaacaaacg ggggggtgtc g                                      31


<210>  10
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  10
ggcccccgag gccagcatga cgttatct                                          28


<210>  11
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
gagtaaccgc ccccccccca tgccaccctc ac                                     32


<210>  12
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  12
gtgttttact gccactacac ccccggggaa c                                      31


<210>  13
<211>  121
<212>  PRT
<213>  Mus musculus


<220>
<223>  anti-mPD-1 scFv heavy chain variable region

<400>  13

Glu Val Met Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Leu Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser Gly Gly Gly Gly Asp Thr Tyr Phe Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Val Lys Asn Asn Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Val Arg Phe Gly Gly Ala Gly Tyr Tyr Trp Tyr Phe Asp Val Trp Gly 
            100                 105                 110         


Ala Gly Thr Thr Val Thr Val Ser Ser 
        115                 120     


<210>  14
<211>  111
<212>  PRT
<213>  Mus musculus


<220>
<223>  anti-mPD-1 scFv light chain variable region

<400>  14

Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Lys Ser Val Asp Asp Ser 
            20                  25                  30          


Gly Ile Ser Phe Met His Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Ala Ala Ser Asn Gln Gly Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Arg Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile His 
65                  70                  75                  80  


Pro Met Glu Glu Asp Asp Thr Ala Met Tyr Phe Cys His Gln Thr Lys 
                85                  90                  95      


Glu Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


