                         SEQUENCE LISTING

<110>  GlaxoSmithKline Intellectual Property Development Limited
 
<120>  IL-7  BINDING PROTEINS AND THEIR USE IN MEDICAL THERAPY

<130>  PU66978

<150>  US 63/120564
<151>  2020-12-02

<160>  47    

<170>  PatentIn version 3.5

<210>  1
<211>  177
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human IL-7 Sequence with leader sequence

<400>  1

Met Phe His Val Ser Phe Arg Tyr Ile Phe Gly Leu Pro Pro Leu Ile 
1               5                   10                  15      


Leu Val Leu Leu Pro Val Ala Ser Ser Asp Cys Asp Ile Glu Gly Lys 
            20                  25                  30          


Asp Gly Lys Gln Tyr Glu Ser Val Leu Met Val Ser Ile Asp Gln Leu 
        35                  40                  45              


Leu Asp Ser Met Lys Glu Ile Gly Ser Asn Cys Leu Asn Asn Glu Phe 
    50                  55                  60                  


Asn Phe Phe Lys Arg His Ile Cys Asp Ala Asn Lys Glu Gly Met Phe 
65                  70                  75                  80  


Leu Phe Arg Ala Ala Arg Lys Leu Arg Gln Phe Leu Lys Met Asn Ser 
                85                  90                  95      


Thr Gly Asp Phe Asp Leu His Leu Leu Lys Val Ser Glu Gly Thr Thr 
            100                 105                 110         


Ile Leu Leu Asn Cys Thr Gly Gln Val Lys Gly Arg Lys Pro Ala Ala 
        115                 120                 125             


Leu Gly Glu Ala Gln Pro Thr Lys Ser Leu Glu Glu Asn Lys Ser Leu 
    130                 135                 140                 


Lys Glu Gln Lys Lys Leu Asn Asp Leu Cys Phe Leu Lys Arg Leu Leu 
145                 150                 155                 160 


Gln Glu Ile Lys Thr Cys Trp Asn Lys Ile Leu Met Gly Thr Lys Glu 
                165                 170                 175     


His 
    


<210>  2
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DRSPAI-L7B heavy chain

<400>  2

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Val His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Ala Ala Ile Trp Thr Gly Gly Ser Thr Asp Tyr Asn Ser Ala Phe Ser 
    50                  55                  60                  


Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asn Gly Tyr Gly Glu Ser Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His 
    210                 215                 220                 


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Ala Gly Ala Pro Ser Val 
225                 230                 235                 240 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                245                 250                 255     


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
            260                 265                 270         


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        275                 280                 285             


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
    290                 295                 300                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
305                 310                 315                 320 


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
                325                 330                 335     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
            340                 345                 350         


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
        355                 360                 365             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    370                 375                 380                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
385                 390                 395                 400 


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
                405                 410                 415     


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            420                 425                 430         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445         


<210>  3
<211>  218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DRSPAI-L7B light chain

<400>  3

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Glu Ser Leu Asp His Asp 
            20                  25                  30          


Gly Asp Ser Tyr Ile Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Met Gly Ser Asn Val Glu Phe Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Val Asp Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
        115                 120                 125             


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
    130                 135                 140                 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
145                 150                 155                 160 


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
                165                 170                 175     


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
            180                 185                 190         


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
        195                 200                 205             


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215             


<210>  4
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DRSPAI-L7B Vh

<400>  4

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Val His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Ala Ala Ile Trp Thr Gly Gly Ser Thr Asp Tyr Asn Ser Ala Phe Ser 
    50                  55                  60                  


Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asn Gly Tyr Gly Glu Ser Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  5
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DRSPAI-L7B Vl

<400>  5

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Glu Ser Leu Asp His Asp 
            20                  25                  30          


Gly Asp Ser Tyr Ile Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Met Gly Ser Asn Val Glu Phe Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Val Asp Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  6
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DRSPAI-L7B CDRH1

<400>  6

Ser Tyr Gly Val His 
1               5   


<210>  7
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DRSPAI-L7B CDRH2

<400>  7

Ala Ile Trp Thr Gly Gly Ser Thr Asp Tyr Asn Ser Ala Phe Ser Ser 
1               5                   10                  15      


<210>  8
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DRSPAI-L7B CDRH3

<400>  8

Asn Gly Tyr Gly Glu Ser Phe Ala Tyr 
1               5                   


<210>  9
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DRSPAI-L7B CDRL1

<400>  9

Lys Ala Ser Glu Ser Leu Asp His Asp Gly Asp Ser Tyr Ile Asn 
1               5                   10                  15  


<210>  10
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DRSPAI-L7B CDRL2

<400>  10

Met Gly Ser Asn Val Glu Phe 
1               5           


<210>  11
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DRSPAI-L7B CDRL3

<400>  11

Gln Gln Ser Asn Val Asp Pro Leu Thr 
1               5                   


<210>  12
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DRSPAI-L7B 1st protected site

<400>  12

Phe Lys Arg His Ile Cys Asp Ala Asn Lys Glu Gly Met Phe Leu 
1               5                   10                  15  


<210>  13
<211>  654
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence encoding DRSPAI-L7B light chain

<400>  13
gacatccaga tgacccagag ccctagcagc ctgagcgcca gcgtgggaga cagggtgacc       60

atcacctgca aggccagcga gtccctggac cacgacggcg acagctacat caactggtac      120

cagcagaagc ccggcaaggc ccccaagctg ctgatctaca tgggcagcaa cgtggagttc      180

ggcgtgcccg ccaggtttag cggcagcggc agcggcaccg acttcaccct gaccatcagc      240

agcctccagc ccgaggactt cgccacctac tactgccagc agagcaacgt ggaccccctg      300

actttcggcg gcggcaccaa ggtggagatt aagcgtacgg tggccgcccc cagcgtgttc      360

atcttccccc ccagcgatga gcagctgaag agcggcaccg ccagcgtggt gtgtctgctg      420

aacaacttct acccccggga ggccaaggtg cagtggaagg tggacaatgc cctgcagagc      480

ggcaacagcc aggagagcgt gaccgagcag gacagcaagg actccaccta cagcctgagc      540

agcaccctga ccctgagcaa ggccgactac gagaagcaca aggtgtacgc ctgtgaggtg      600

acccaccagg gcctgtccag ccccgtgacc aagagcttca accggggcga gtgc            654


<210>  14
<211>  1341
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence encoding DRSPAI-L7B heavy chain

<400>  14
caggtgcagc tggtggagag cggcggcggc gtggtccagc ccggaaggag cctgaggctg       60

agctgcgccg ccagcggctt caccttcagc agctacgggg tccactgggt gaggcaggcc      120

cccggaaagg gcctggagtg gctggccgcc atctggaccg gcggctccac cgactacaac      180

agcgccttca gcagcaggtt caccatcagc agggacaact ccaagaacac cctgtacctg      240

cagatgaaca gcctcagggc cgaggacacc gccgtgtact attgcgcaag gaacggctac      300

ggcgagagct tcgcctactg gggccagggc accctggtga ccgtgagcag cgccagcacc      360

aagggcccca gcgtgttccc cctggccccc agcagcaaga gcaccagcgg cggcacagcc      420

gccctgggct gcctggtgaa ggactacttc cccgagcccg tgaccgtgtc ctggaacagc      480

ggagccctga ccagcggcgt gcacaccttc cccgccgtgc tgcagagcag cggcctgtac      540

agcctgagca gcgtggtgac cgtgcccagc agcagcctgg gcacccagac ctacatctgt      600

aacgtgaacc acaagcccag caacaccaag gtggacaaga aggtggagcc caagagctgt      660

gacaagaccc acacctgccc cccctgccct gcccccgagc tggccggagc ccccagcgtg      720

ttcctgttcc cccccaagcc taaggacacc ctgatgatca gcagaacccc cgaggtgacc      780

tgtgtggtgg tggatgtgag ccacgaggac cctgaggtga agttcaactg gtacgtggac      840

ggcgtggagg tgcacaatgc caagaccaag cccagggagg agcagtacaa cagcacctac      900

cgggtggtgt ccgtgctgac cgtgctgcac caggattggc tgaacggcaa ggagtacaag      960

tgtaaggtgt ccaacaaggc cctgcctgcc cctatcgaga aaaccatcag caaggccaag     1020

ggccagccca gagagcccca ggtgtacacc ctgcccccta gcagagatga gctgaccaag     1080

aaccaggtgt ccctgacctg cctggtgaag ggcttctacc ccagcgacat cgccgtggag     1140

tgggagagca acggccagcc cgagaacaac tacaagacca ccccccctgt gctggacagc     1200

gatggcagct tcttcctgta cagcaagctg accgtggaca agagcagatg gcagcagggc     1260

aacgtgttca gctgctccgt gatgcacgag gccctgcaca atcactacac ccagaagagc     1320

ctgagcctgt cccctggcaa g                                               1341


<210>  15
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence encoding signaling peptide

<400>  15
atgggctggt cctgcatcat cctgtttctg gtggccaccg ccaccggtgt gcacagc          57


<210>  16
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DRSPAI-L7B 2nd protected site

<400>  16

Phe Lys Arg His Ile Cys Asp Ala Asn Lys Glu Gly Met Phe 
1               5                   10                  


<210>  17
<211>  152
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IL-7 Sequence (without secretory leader)

<400>  17

Asp Cys Asp Ile Glu Gly Lys Asp Gly Lys Gln Tyr Glu Ser Val Leu 
1               5                   10                  15      


Met Val Ser Ile Asp Gln Leu Leu Asp Ser Met Lys Glu Ile Gly Ser 
            20                  25                  30          


Asn Cys Leu Asn Asn Glu Phe Asn Phe Phe Lys Arg His Ile Cys Asp 
        35                  40                  45              


Ala Asn Lys Glu Gly Met Phe Leu Phe Arg Ala Ala Arg Lys Leu Arg 
    50                  55                  60                  


Gln Phe Leu Lys Met Asn Ser Thr Gly Asp Phe Asp Leu His Leu Leu 
65                  70                  75                  80  


Lys Val Ser Glu Gly Thr Thr Ile Leu Leu Asn Cys Thr Gly Gln Val 
                85                  90                  95      


Lys Gly Arg Lys Pro Ala Ala Leu Gly Glu Ala Gln Pro Thr Lys Ser 
            100                 105                 110         


Leu Glu Glu Asn Lys Ser Leu Lys Glu Gln Lys Lys Leu Asn Asp Leu 
        115                 120                 125             


Cys Phe Leu Lys Arg Leu Leu Gln Glu Ile Lys Thr Cys Trp Asn Lys 
    130                 135                 140                 


Ile Leu Met Gly Thr Lys Glu His 
145                 150         


<210>  18
<211>  218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1290 light chain

<400>  18

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Asp Asp Asp 
            20                  25                  30          


Gly Asp Ser Phe Ile Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Val Ala Ser Asn Leu Glu Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
        115                 120                 125             


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
    130                 135                 140                 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
145                 150                 155                 160 


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
                165                 170                 175     


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
            180                 185                 190         


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
        195                 200                 205             


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215             


<210>  19
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1290 heavy chain

<400>  19

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Leu His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Ala Ala Ile Trp Thr Gly Gly Ser Thr Asp Tyr Asn Ala Ala Phe Ile 
    50                  55                  60                  


Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asn Gly Tyr Gly Glu Ser Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His 
    210                 215                 220                 


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Ala Gly Ala Pro Ser Val 
225                 230                 235                 240 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                245                 250                 255     


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
            260                 265                 270         


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        275                 280                 285             


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
    290                 295                 300                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
305                 310                 315                 320 


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
                325                 330                 335     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
            340                 345                 350         


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
        355                 360                 365             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    370                 375                 380                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
385                 390                 395                 400 


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
                405                 410                 415     


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            420                 425                 430         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445         


<210>  20
<211>  218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1291 light chain

<400>  20

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser His Ser Val Asp Asp Asp 
            20                  25                  30          


Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Met Ala Ser Asn Leu Glu Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
        115                 120                 125             


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
    130                 135                 140                 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
145                 150                 155                 160 


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
                165                 170                 175     


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
            180                 185                 190         


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
        195                 200                 205             


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215             


<210>  21
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1291 heavy chain

<400>  21

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Leu His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Ala Ala Ile Trp Thr Gly Gly Ser Thr Asp Tyr Asn Ala Glu Phe Ser 
    50                  55                  60                  


Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asn Gly Tyr Gly Glu Ser Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His 
    210                 215                 220                 


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Ala Gly Ala Pro Ser Val 
225                 230                 235                 240 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                245                 250                 255     


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
            260                 265                 270         


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        275                 280                 285             


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
    290                 295                 300                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
305                 310                 315                 320 


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
                325                 330                 335     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
            340                 345                 350         


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
        355                 360                 365             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    370                 375                 380                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
385                 390                 395                 400 


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
                405                 410                 415     


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            420                 425                 430         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445         


<210>  22
<211>  218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1294 light chain

<400>  22

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Asp Asp Asp 
            20                  25                  30          


Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Met Ala Ser Asn Leu Glu Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
        115                 120                 125             


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
    130                 135                 140                 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
145                 150                 155                 160 


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
                165                 170                 175     


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
            180                 185                 190         


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
        195                 200                 205             


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215             


<210>  23
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1294 heavy chain

<400>  23

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 
            20                  25                  30          


Gly Val His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Ala Ala Ile Trp Ser Gly Gly Ser Thr Asp Tyr Asn Val Ala Phe Ser 
    50                  55                  60                  


Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asn Gly Tyr Gly Glu Ser Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His 
    210                 215                 220                 


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Ala Gly Ala Pro Ser Val 
225                 230                 235                 240 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                245                 250                 255     


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
            260                 265                 270         


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        275                 280                 285             


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
    290                 295                 300                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
305                 310                 315                 320 


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
                325                 330                 335     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
            340                 345                 350         


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
        355                 360                 365             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    370                 375                 380                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
385                 390                 395                 400 


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
                405                 410                 415     


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            420                 425                 430         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445         


<210>  24
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1290 light chain

<400>  24

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Asp Asp Asp 
            20                  25                  30          


Gly Asp Ser Phe Ile Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Val Ala Ser Asn Leu Glu Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  25
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1290 Heavy chain

<400>  25

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Leu His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Ala Ala Ile Trp Thr Gly Gly Ser Thr Asp Tyr Asn Ala Ala Phe Ile 
    50                  55                  60                  


Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asn Gly Tyr Gly Glu Ser Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  26
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1290 CDRH1

<400>  26

Ser Tyr Gly Leu His 
1               5   


<210>  27
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1290 CDRH2

<400>  27

Ala Ile Trp Thr Gly Gly Ser Thr Asp Tyr Asn Ala Ala Phe Ile Ser 
1               5                   10                  15      


<210>  28
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1290 CDRH3

<400>  28

Asn Gly Tyr Gly Glu Ser Phe Ala Tyr 
1               5                   


<210>  29
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1290 CDRL1

<400>  29

Lys Ala Ser Gln Ser Val Asp Asp Asp Gly Asp Ser Phe Ile Asn 
1               5                   10                  15  


<210>  30
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1290 CDRL2

<400>  30

Val Ala Ser Asn Leu Glu Ser 
1               5           


<210>  31
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1290 CDRL3

<400>  31

Gln Gln Ser Asn Glu Asp Pro Leu Thr 
1               5                   


<210>  32
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1291 light chain

<400>  32

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser His Ser Val Asp Asp Asp 
            20                  25                  30          


Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Met Ala Ser Asn Leu Glu Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  33
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1291 heavy chain

<400>  33

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Leu His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Ala Ala Ile Trp Thr Gly Gly Ser Thr Asp Tyr Asn Ala Glu Phe Ser 
    50                  55                  60                  


Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asn Gly Tyr Gly Glu Ser Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  34
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1291 CDRH1

<400>  34

Ser Tyr Gly Leu His 
1               5   


<210>  35
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1291 CDRH2

<400>  35

Ala Ile Trp Thr Gly Gly Ser Thr Asp Tyr Asn Ala Glu Phe Ser Ser 
1               5                   10                  15      


<210>  36
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1291 CDRH3

<400>  36

Asn Gly Tyr Gly Glu Ser Phe Ala Tyr 
1               5                   


<210>  37
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1291 CDRL1

<400>  37

Lys Ala Ser His Ser Val Asp Asp Asp Gly Asp Ser Tyr Met Asn 
1               5                   10                  15  


<210>  38
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1291 CDRL2

<400>  38

Met Ala Ser Asn Leu Glu Ser 
1               5           


<210>  39
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1291 CDRL3

<400>  39

Gln Gln Ser Asn Glu Asp Pro Leu Thr 
1               5                   


<210>  40
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1294  light chain

<400>  40

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Asp Asp Asp 
            20                  25                  30          


Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Met Ala Ser Asn Leu Glu Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Asn 
                85                  90                  95      


Glu Asp Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     


<210>  41
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1294 heavy chain

<400>  41

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 
            20                  25                  30          


Gly Val His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Ala Ala Ile Trp Ser Gly Gly Ser Thr Asp Tyr Asn Val Ala Phe Ser 
    50                  55                  60                  


Ser Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asn Gly Tyr Gly Glu Ser Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  42
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1294 CDRH1

<400>  42

Thr Tyr Gly Val His 
1               5   


<210>  43
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1294 CDRH2

<400>  43

Ala Ile Trp Ser Gly Gly Ser Thr Asp Tyr Asn Val Ala Phe Ser Ser 
1               5                   10                  15      


<210>  44
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1294 CDRH3

<400>  44

Asn Gly Tyr Gly Glu Ser Phe Ala Tyr 
1               5                   


<210>  45
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1294 CDRL1

<400>  45

Lys Ala Ser Gln Ser Val Asp Asp Asp Gly Asp Ser Tyr Met Asn 
1               5                   10                  15  


<210>  46
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1294 CDRL2

<400>  46

Met Ala Ser Asn Leu Glu Ser 
1               5           


<210>  47
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A1294 CDRL3

<400>  47

Gln Gln Ser Asn Glu Asp Pro Leu Thr 
1               5                   


