                         SEQUENCE LISTING

<110>  The Johns Hopkins University
 
<120>  METHODS AND MATERIALS FOR TREATING T CELL CANCERS

<130>  44807-0384WO1

<150>  63/119,753
<151>  2020-12-01

<160>  69    

<170>  PatentIn version 3.5

<210>  1
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV5-5 VL CDR1

<400>  1

Cys Ser Ala Ser Gln Gly Ile Ser Asn Tyr Leu Asn 
1               5                   10          


<210>  2
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV5-5 VL CDR2

<400>  2

Thr Ser Ser Leu His Ser Gly Val 
1               5               


<210>  3
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV5-5 VL CDR3

<400>  3

Gln Gln Tyr Ser Lys Leu Pro Arg Thr 
1               5                   


<210>  4
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV5-5 VH CDR1

<400>  4

Ala Tyr Gly Val Asn 
1               5   


<210>  5
<211>  13
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV5-5 VH CDR2

<400>  5

Trp Gly Asp Gly Asn Thr Asp Tyr Asn Ser Ala Leu Lys 
1               5                   10              


<210>  6
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV5-5 VH CDR3

<400>  6

Ala Thr Leu Tyr Ala Met Asp Tyr 
1               5               


<210>  7
<211>  107
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV5-5 VL

<400>  7

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Gly Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Lys Leu Pro Arg 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  8
<211>  120
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV5-5 VH

<400>  8

Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Ala Tyr 
            20                  25                  30          


Gly Val Asn Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Met Ile Trp Gly Asp Gly Asn Thr Asp Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  


Ser Arg Leu Ser Ile Ser Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  


Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asp Arg Val Thr Ala Thr Leu Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser 
        115                 120 


<210>  9
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV12 VL CDR1

<400>  9

Cys Arg Ala Ser Ser Ser Val Asn Tyr Ile Tyr Trp 
1               5                   10          


<210>  10
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV12 VL CDR2

<400>  10

Tyr Thr Ser Asn Leu Ala Pro Gly Val Pro 
1               5                   10  


<210>  11
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV12 VL CDR3

<400>  11

Gln Gln Phe Thr Ser Ser Pro Phe Thr 
1               5                   


<210>  12
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV12 VH CDR1

<400>  12

Asn Phe Gly Met His 
1               5   


<210>  13
<211>  17
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV12 VH CDR2

<400>  13

Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Thr Leu Lys 
1               5                   10                  15      


Gly 
    


<210>  14
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV12 VH CDR3

<400>  14

Arg Gly Glu Gly Ala Met Asp Tyr 
1               5               


<210>  15
<211>  106
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV12 VL

<400>  15

Glu Asn Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Leu Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Arg Ala Ser Ser Ser Val Asn Tyr Ile 
            20                  25                  30          


Tyr Trp Tyr Gln Gln Lys Ser Asp Ala Ser Pro Lys Leu Trp Ile Tyr 
        35                  40                  45              


Tyr Thr Ser Asn Leu Ala Pro Gly Val Pro Thr Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Asn Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Gly Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Phe Thr Ser Ser Pro Phe Thr 
                85                  90                  95      


Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105     


<210>  16
<211>  117
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV12 VH

<400>  16

Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Arg Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Phe 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Asp Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Thr Leu 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Pro Lys Asn Thr Leu Phe 
65                  70                  75                  80  


Leu Gln Met Thr Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Gly Glu Gly Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  17
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VL CDR1

<400>  17

Arg Ala Ser Gln Asp Ile Arg Asn Tyr Leu Asn 
1               5                   10      


<210>  18
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VL CDR2


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Y at position 1 is optional

<400>  18

Tyr Tyr Thr Ser Arg Leu His Ser 
1               5               


<210>  19
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VL CDR3

<400>  19

Gln Gln Gly Asn Thr Leu Pro Trp Thr 
1               5                   


<210>  20
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VH CDR1

<400>  20

Gly Tyr Thr Met Asn 
1               5   


<210>  21
<211>  17
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VH CDR2

<400>  21

Leu Ile Asn Pro Tyr Lys Gly Val Ser Thr Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Asp 
    


<210>  22
<211>  13
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VH CDR3

<400>  22

Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val 
1               5                   10              


<210>  23
<211>  107
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 UCHT1 VL

<400>  23

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Arg Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Arg Leu His Ser Gly Val Pro Ser Lys Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Trp 
                85                  90                  95      


Thr Phe Ala Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  24
<211>  122
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 UCHT1 VH

<400>  24

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Met Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 
            20                  25                  30          


Thr Met Asn Trp Val Lys Gln Ser His Gly Lys Asn Leu Glu Trp Met 
        35                  40                  45              


Gly Leu Ile Asn Pro Tyr Lys Gly Val Ser Thr Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val Trp 
            100                 105                 110         


Gly Ala Gly Thr Thr Val Thr Val Ser Ser 
        115                 120         


<210>  25
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  polypeptide linker

<400>  25

Gly Gly Gly Gly Ser 
1               5   


<210>  26
<211>  15
<212>  PRT
<213>  artificial

<220>
<223>  polypeptide linker

<400>  26

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  27
<211>  113
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBC1 VL

<400>  27

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Arg Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Arg Val Ser Asn Arg Phe Pro Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Ile Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg 
    


<210>  28
<211>  124
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBC1 VH

<400>  28

Glu Val Arg Leu Gln Gln Ser Gly Pro Asp Leu Ile Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Gly Tyr 
            20                  25                  30          


Val Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Phe Ile Asn Pro Tyr Asn Asp Asp Ile Gln Ser Asn Glu Arg Phe 
    50                  55                  60                  


Arg Gly Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser Thr Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Ala Gly Tyr Asn Phe Asp Gly Ala Tyr Arg Phe Phe Asp 
            100                 105                 110         


Phe Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser 
        115                 120                 


<210>  29
<211>  111
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD19 VL

<400>  29

Asp Ile Gln Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Ile Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Asp Ala Ser Asn Leu Val Ser Gly Ile Pro Pro 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Lys Val Asp Ala Ala Thr Tyr His Cys Gln Gln Ser Thr 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  30
<211>  124
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD19 VH

<400>  30

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Gln Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120                 


<210>  31
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  nucleic acid encoding crRNA targeting the TRA constant region

<400>  31
agagtctctc agctggtaca                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  nucleic acid encoding crRNA targeting the TRB constant region

<400>  32
agaaggtggc cgagaccctc                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  PCR primer

<400>  33
gcctaagttg gggagaccac                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  PCR primer

<400>  34
gaagcaagga aacagcctgc                                                   20


<210>  35
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  PCR primer

<400>  35
tcgctgtgtt tgagccatca ga                                                22


<210>  36
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  PCR primer

<400>  36
atgaaccaca ggtgcccaat tc                                                22


<210>  37
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  nucleic acid encoding sgRNA targeting CD19

<400>  37
cgaggaacct ctagtggtga                                                   20


<210>  38
<211>  107
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV5-5 VL


<220>
<221>  MISC_FEATURE
<222>  (79)..(79)
<223>  X at residue 78 is a pyrrolysine

<400>  38

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Gly Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Xaa Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Lys Leu Pro Arg 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Gln Ile Thr 
            100                 105         


<210>  39
<211>  120
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV5-5 VH

<400>  39

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Arg Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Ala Tyr 
            20                  25                  30          


Gly Val Asn Trp Val Arg Gln Pro Pro Gly Arg Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Met Ile Trp Gly Asp Gly Asn Thr Asp Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  


Ser Arg Val Thr Met Leu Lys Asp Thr Ser Lys Asn Gln Phe Ser Leu 
65                  70                  75                  80  


Arg Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asp Arg Val Thr Ala Thr Leu Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Ser Leu Val Thr Val Ser Ser 
        115                 120 


<210>  40
<211>  106
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV12 VL

<400>  40

Asp Ile Gln Met Thr Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
1               5                   10                  15      


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Ser Ser Val Asn Tyr 
            20                  25                  30          


Ile Tyr Trp Tyr Gln Gln Thr Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Asn Leu Ala Pro Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Phe Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Thr Tyr Tyr Cys Gln Gln Phe Thr Ser Ser Pro Phe Thr 
                85                  90                  95      


Phe Gly Ser Gly Thr Lys Leu Gln Ile Thr 
            100                 105     


<210>  41
<211>  117
<212>  PRT
<213>  artificial

<220>
<223>  anti-TRBV12 VH

<400>  41

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Arg Lys Leu Ser Cys Ser Ser Ser Gly Phe Thr Phe Ser Asn Phe 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Thr Leu 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Phe 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Arg Gly Glu Gly Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  42
<211>  107
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 UCHT1v9 VL

<400>  42

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Arg Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Arg Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Asn Thr Leu Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  43
<211>  122
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 UCHT1v9 VH

<400>  43

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 
            20                  25                  30          


Thr Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Leu Ile Asn Pro Tyr Lys Gly Val Ser Thr Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Val Asp Lys Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val Trp 
            100                 105                 110         


Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120         


<210>  44
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VL CDR1

<400>  44

Arg Ala Ser Ser Ser Val Ser Tyr Met Asn 
1               5                   10  


<210>  45
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VL CDR1

<400>  45

Ser Ala Ser Ser Ser Val Ser Tyr Met Asn 
1               5                   10  


<210>  46
<211>  14
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VL CDR1

<400>  46

Arg Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn 
1               5                   10                  


<210>  47
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VL CDR1

<400>  47

Arg Ala Ser Gln Ser Val Ser Tyr Met Asn 
1               5                   10  


<210>  48
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VL CDR2

<400>  48

Asp Thr Ser Lys Val Ala Ser 
1               5           


<210>  49
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VL CDR2

<400>  49

Asp Thr Ser Lys Leu Ala Ser 
1               5           


<210>  50
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VL CDR2

<400>  50

Gly Thr Asn Lys Arg Ala Pro 
1               5           


<210>  51
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VL CDR3

<400>  51

Gln Gln Trp Ser Ser Asn Pro Leu Thr 
1               5                   


<210>  52
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VL CDR3

<400>  52

Gln Gln Trp Ser Ser Asn Pro Phe Thr 
1               5                   


<210>  53
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VL CDR3

<400>  53

Ala Leu Trp Tyr Ser Asn Leu Trp Val 
1               5                   


<210>  54
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VH CDR1

<400>  54

Arg Tyr Thr Met His 
1               5   


<210>  55
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VH CDR1

<400>  55

Thr Tyr Ala Met Asn 
1               5   


<210>  56
<211>  16
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VH CDR2

<400>  56

Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


<210>  57
<211>  19
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VH CDR2

<400>  57

Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser 
1               5                   10                  15      


Val Lys Asp 
            


<210>  58
<211>  17
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VH CDR2

<400>  58

Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  59
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VH CDR3

<400>  59

Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr 
1               5                   10  


<210>  60
<211>  14
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 VH CDR3

<400>  60

His Gly Asn Phe Gly Asn Ser Tyr Val Ser Trp Phe Ala Tyr 
1               5                   10                  


<210>  61
<211>  106
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 L2K-07 VL

<400>  61

Asp Ile Gln Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


Asn Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr 
                85                  90                  95      


Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105     


<210>  62
<211>  119
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 L2K-07 VH

<400>  62

Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser 
        115                 


<210>  63
<211>  106
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 OKT3 VL

<400>  63

Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


Asn Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ala His Phe Arg Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Gly Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr 
                85                  90                  95      


Phe Gly Ser Gly Thr Lys Leu Glu Ile Asn 
            100                 105     


<210>  64
<211>  119
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 OKT3 VH

<400>  64

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser 
        115                 


<210>  65
<211>  109
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 hXR32 VL

<400>  65

Gln Ala Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly 
1               5                   10                  15      


Thr Val Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser 
            20                  25                  30          


Asn Tyr Ala Asn Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly 
        35                  40                  45              


Leu Ile Gly Gly Thr Asn Lys Arg Ala Pro Trp Thr Pro Ala Arg Phe 
    50                  55                  60                  


Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Ile Thr Gly Ala 
65                  70                  75                  80  


Gln Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn 
                85                  90                  95      


Leu Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 


<210>  66
<211>  125
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 hXR32 VH

<400>  66

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Ser 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Arg His Gly Asn Phe Gly Asn Ser Tyr Val Ser Trp Phe 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 125 


<210>  67
<211>  106
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 diL2K VL

<400>  67

Asp Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Tyr Met 
            20                  25                  30          


Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Val Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Asn Ser Leu Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105     


<210>  68
<211>  119
<212>  PRT
<213>  artificial

<220>
<223>  anti-CD3 diL2K VH

<400>  68

Asp Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Thr Thr Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Val Thr Val Ser Ser 
        115                 


<210>  69
<211>  16
<212>  PRT
<213>  artificial

<220>
<223>  polypeptide linker

<400>  69

Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp 
1               5                   10                  15      


