                         SEQUENCE LISTING

<110>  Brigham Young University
 
<120>  METHOD FOR DETECTION OF VIRAL INFECTIONS USING SPLIT ENZYMES IN 
       AN OXIDATIVE ENVIRONMENT

<130>  82570-349840

<140>  PCT/US2021/072739
<141>  2021-12-03

<150>  63/121,784
<151>  2020-12-04

<150>  63/260,247
<151>  2021-08-13

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  481
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Split HRP 1 with COVID19 scfv

<400>  1

Met His His His His His His Met Glu Val Gln Leu Val Glu Ser Gly 
1               5                   10                  15      


Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala 
            20                  25                  30          


Ser Gly Phe Ile Val Ser Ser Asn Tyr Met Ser Trp Val Arg Gln Ala 
        35                  40                  45              


Pro Gly Lys Gly Leu Glu Trp Val Ser Val Ile Tyr Ser Gly Gly Ser 
    50                  55                  60                  


Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg His 
65                  70                  75                  80  


Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu 
                85                  90                  95      


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu Ala Tyr Gly Met Asp Val 
            100                 105                 110         


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln 
    130                 135                 140                 


Ser Pro Ser Phe Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
145                 150                 155                 160 


Cys Arg Ala Ser Gln Gly Ile Ser Ser Tyr Leu Ala Trp Tyr Gln Gln 
                165                 170                 175     


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Thr Leu 
            180                 185                 190         


Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu 
        195                 200                 205             


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
    210                 215                 220                 


Tyr Cys Gln Gln Leu Asn Ser Tyr Pro Pro Tyr Thr Phe Gly Gln Gly 
225                 230                 235                 240 


Thr Lys Leu Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
                245                 250                 255     


Gly Gly Gly Gly Ser Gln Leu Thr Pro Thr Phe Tyr Asp Asn Ser Cys 
            260                 265                 270         


Pro Asn Val Ser Asn Ile Val Arg Asp Ile Ile Val Asn Glu Leu Arg 
        275                 280                 285             


Ser Asp Pro Arg Ile Ala Ala Ser Ile Leu Arg Leu His Phe His Asp 
    290                 295                 300                 


Cys Phe Val Asn Gly Cys Asp Ala Ser Ile Leu Leu Asp Asn Thr Thr 
305                 310                 315                 320 


Ser Phe Arg Thr Glu Lys Asp Ala Phe Gly Asn Ala Asn Ser Ala Arg 
                325                 330                 335     


Gly Phe Ser Val Ile Asp Arg Met Lys Ala Ala Val Glu Ser Ala Cys 
            340                 345                 350         


Pro Gly Thr Val Ser Cys Ala Asp Leu Leu Thr Ile Ala Ala Gln Gln 
        355                 360                 365             


Ser Val Thr Leu Ala Gly Gly Pro Ser Trp Arg Val Pro Leu Gly Arg 
    370                 375                 380                 


Arg Asp Ser Leu Gln Ala Phe Leu Asp Leu Ala Asn Ala Asn Leu Pro 
385                 390                 395                 400 


Ala Pro Phe Phe Thr Leu Pro Gln Leu Lys Asp Ser Phe Arg Asn Val 
                405                 410                 415     


Gly Leu Asn Arg Ser Ser Asp Leu Val Ala Leu Ser Gly Gly His Thr 
            420                 425                 430         


Phe Gly Lys Ser Gln Cys Arg Phe Ile Met Asp Arg Leu Tyr Asn Phe 
        435                 440                 445             


Ser Asn Thr Gly Leu Pro Asp Pro Thr Leu Asn Thr Thr Tyr Leu Gln 
    450                 455                 460                 


Thr Leu Arg Gly Leu Cys Pro Leu Asn Gly Gly Ser Gly Lys Asp Glu 
465                 470                 475                 480 


Leu 
    


<210>  2
<211>  379
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Split HRP 2 with COVID19 scfv

<400>  2

Met His His His His His His Met Gln Val Gln Leu Val Gln Ser Gly 
1               5                   10                  15      


Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala 
            20                  25                  30          


Ser Gly Tyr Thr Phe Thr Gly Tyr Tyr Met His Trp Val Arg Gln Ala 
        35                  40                  45              


Pro Gly Gln Gly Leu Glu Trp Met Gly Arg Ile Asn Pro Asn Ser Gly 
    50                  55                  60                  


Gly Thr Asn Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met Thr Arg 
65                  70                  75                  80  


Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser 
                85                  90                  95      


Asp Asp Thr Ala Val Tyr Tyr Cys Ala Arg Val Pro Tyr Cys Ser Ser 
            100                 105                 110         


Thr Ser Cys His Arg Asp Trp Tyr Phe Asp Leu Trp Gly Arg Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Leu Ser Leu 
145                 150                 155                 160 


Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln 
                165                 170                 175     


Ser Leu Leu Asp Ser Asp Asp Gly Asn Thr Tyr Leu Asp Trp Tyr Leu 
            180                 185                 190         


Gln Lys Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr Thr Leu Ser Tyr 
        195                 200                 205             


Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr 
    210                 215                 220                 


Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val 
225                 230                 235                 240 


Tyr Tyr Cys Met Gln Arg Ile Glu Phe Pro Leu Thr Phe Gly Gly Gly 
                245                 250                 255     


Thr Lys Val Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            260                 265                 270         


Gly Gly Gly Gly Ser Asn Leu Ser Ala Leu Val Asp Phe Asp Leu Arg 
        275                 280                 285             


Thr Pro Thr Ile Phe Asp Asn Lys Tyr Tyr Val Asn Leu Glu Glu Gln 
    290                 295                 300                 


Lys Gly Leu Ile Gln Ser Asp Gln Glu Leu Phe Ser Ser Pro Asp Ala 
305                 310                 315                 320 


Thr Asp Thr Ile Pro Leu Val Arg Ser Phe Ala Asn Ser Thr Gln Thr 
                325                 330                 335     


Phe Phe Asn Ala Phe Val Glu Ala Met Asp Arg Met Gly Asn Ile Thr 
            340                 345                 350         


Pro Leu Thr Gly Thr Gln Gly Gln Ile Arg Arg Asn Cys Arg Val Val 
        355                 360                 365             


Asn Ser Asn Ser Gly Ser Gly Lys Asp Glu Leu 
    370                 375                 


<210>  3
<211>  534
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Split Apex 1 with COVID19 scfv

<400>  3

Met Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15      


Gly Gly Gly Gly Ser Met Gly Lys Ser Tyr Pro Thr Val Ser Ala Asp 
            20                  25                  30          


Tyr Gln Asp Ala Val Glu Lys Ala Lys Lys Arg Leu Gly Gly Phe Ile 
        35                  40                  45              


Ala Glu Lys Arg Cys Ala Pro Leu Met Leu Arg Leu Ala Phe His Ser 
    50                  55                  60                  


Ala Gly Thr Phe Asp Lys Arg Thr Lys Thr Gly Gly Pro Phe Gly Thr 
65                  70                  75                  80  


Ile Arg Tyr Pro Ala Glu Leu Ala His Ser Ala Asn Ser Gly Leu Asp 
                85                  90                  95      


Ile Ala Val Arg Leu Leu Glu Pro Leu Lys Ala Glu Phe Pro Ile Leu 
            100                 105                 110         


Ser Tyr Ala Asp Phe Tyr Gln Leu Ala Gly Val Val Ala Val Glu Val 
        115                 120                 125             


Thr Gly Gly Pro Lys Val Pro Phe His Pro Gly Arg Glu Asp Lys Pro 
    130                 135                 140                 


Glu Leu Pro Pro Glu Gly Arg Leu Pro Asp Pro Thr Lys Gly Ser Asp 
145                 150                 155                 160 


His Leu Arg Asp Val Phe Gly Lys Ala Met Gly Leu Thr Asp Gln Asp 
                165                 170                 175     


Ile Val Ala Leu Ser Gly Gly His Thr Leu Gly Ala Ala His Lys Glu 
            180                 185                 190         


Arg Ser Gly Phe Glu Gly Pro Trp Thr Ser Asn Pro Leu Val Phe Asp 
        195                 200                 205             


Asn Ser Tyr Phe Thr Glu Leu Leu Ser Gly Glu Lys Glu Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Cys Ser Gly Gly 
225                 230                 235                 240 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                245                 250                 255     


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Asp Ile Gln 
            260                 265                 270         


Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly Glu Pro Ala 
        275                 280                 285             


Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu Asp Ser Asp Asp Gly 
    290                 295                 300                 


Asn Thr Tyr Leu Asp Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Gln 
305                 310                 315                 320 


Leu Leu Ile Tyr Thr Leu Ser Tyr Arg Ala Ser Gly Val Pro Asp Arg 
                325                 330                 335     


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg 
            340                 345                 350         


Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln Arg Ile Glu 
        355                 360                 365             


Phe Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Ser Gly 
    370                 375                 380                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val 
385                 390                 395                 400 


Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val 
                405                 410                 415     


Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Gly Tyr Tyr Met 
            420                 425                 430         


His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met Gly Arg 
        435                 440                 445             


Ile Asn Pro Asn Ser Gly Gly Thr Asn Tyr Ala Gln Lys Phe Gln Gly 
    450                 455                 460                 


Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu 
465                 470                 475                 480 


Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
                485                 490                 495     


Val Pro Tyr Cys Ser Ser Thr Ser Cys His Arg Asp Trp Tyr Phe Asp 
            500                 505                 510         


Leu Trp Gly Arg Gly Thr Leu Val Thr Val Ser Gly Gly Gly Gly Ser 
        515                 520                 525             


His His His His His His 
    530                 


<210>  4
<211>  362
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Split Apex 2 with COVID19 scfv

<400>  4

Met His His His His His His Gly Gly Gly Gly Ser Met Glu Val Gln 
1               5                   10                  15      


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg 
            20                  25                  30          


Leu Ser Cys Ala Ala Ser Gly Phe Ile Val Ser Ser Asn Tyr Met Ser 
        35                  40                  45              


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Val Ile 
    50                  55                  60                  


Tyr Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe 
65                  70                  75                  80  


Thr Ile Ser Arg His Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn 
                85                  90                  95      


Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu Ala 
            100                 105                 110         


Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
    130                 135                 140                 


Ile Val Met Thr Gln Ser Pro Ser Phe Leu Ser Ala Ser Val Gly Asp 
145                 150                 155                 160 


Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Ser Tyr Leu 
                165                 170                 175     


Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr 
            180                 185                 190         


Ala Ala Ser Thr Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu 
    210                 215                 220                 


Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Leu Asn Ser Tyr Pro Pro Tyr 
225                 230                 235                 240 


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly Gly Gly Gly Ser 
                245                 250                 255     


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
            260                 265                 270         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Cys Ser Gly Gly Gly Gly Ser 
        275                 280                 285             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Gly Leu Leu Gln 
    290                 295                 300                 


Leu Pro Ser Asp Lys Ala Leu Leu Ser Asp Pro Val Phe Arg Pro Leu 
305                 310                 315                 320 


Val Asp Lys Tyr Ala Ala Asp Glu Asp Ala Phe Phe Ala Asp Tyr Ala 
                325                 330                 335     


Glu Ala His Gln Lys Leu Ser Glu Leu Gly Phe Ala Asp Ala Leu Gln 
            340                 345                 350         


Leu Pro Pro Leu Glu Arg Leu Thr Leu Asp 
        355                 360         


<210>  5
<211>  364
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Split GFP 1 with COVID19 scfv

<400>  5

Met His His His His His His Met Glu Val Gln Leu Val Glu Ser Gly 
1               5                   10                  15      


Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala 
            20                  25                  30          


Ser Gly Phe Ile Val Ser Ser Asn Tyr Met Ser Trp Val Arg Gln Ala 
        35                  40                  45              


Pro Gly Lys Gly Leu Glu Trp Val Ser Val Ile Tyr Ser Gly Gly Ser 
    50                  55                  60                  


Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg His 
65                  70                  75                  80  


Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu 
                85                  90                  95      


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu Ala Tyr Gly Met Asp Val 
            100                 105                 110         


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln 
    130                 135                 140                 


Ser Pro Ser Phe Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
145                 150                 155                 160 


Cys Arg Ala Ser Gln Gly Ile Ser Ser Tyr Leu Ala Trp Tyr Gln Gln 
                165                 170                 175     


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Thr Leu 
            180                 185                 190         


Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu 
        195                 200                 205             


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
    210                 215                 220                 


Tyr Cys Gln Gln Leu Asn Ser Tyr Pro Pro Tyr Thr Phe Gly Gln Gly 
225                 230                 235                 240 


Thr Lys Leu Glu Ile Lys Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
                245                 250                 255     


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            260                 265                 270         


Gly Gly Gly Gly Ser Cys Ser Gly Gly Ser Gly Lys Asn Gly Ile Lys 
        275                 280                 285             


Val Asn Phe Lys Thr Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu 
    290                 295                 300                 


Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
305                 310                 315                 320 


Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp 
                325                 330                 335     


Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala 
            340                 345                 350         


Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Asn 
        355                 360                 


<210>  6
<211>  483
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Split GFP 2 with COVID19 scfv

<400>  6

Met Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15      


Gly Gly Gly Gly Ser Met Ala Ser Lys Gly Glu Glu Leu Phe Thr Gly 
            20                  25                  30          


Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys 
        35                  40                  45              


Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu 
    50                  55                  60                  


Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro 
65                  70                  75                  80  


Thr Leu Val Thr Thr Leu Cys Tyr Gly Val Gln Cys Phe Ser Arg Tyr 
                85                  90                  95      


Pro Asp His Met Lys Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu 
            100                 105                 110         


Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr 
        115                 120                 125             


Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg 
    130                 135                 140                 


Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly 
145                 150                 155                 160 


His Lys Leu Glu Tyr Asn Tyr Asn His Asn Val Leu Ile Met Ala Asp 
                165                 170                 175     


Lys Gln Gly Gly Ser Gly Ser Gly Cys Ser Gly Gly Gly Gly Ser Gly 
            180                 185                 190         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        195                 200                 205             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Met Gln Val Gln Leu Val Gln 
    210                 215                 220                 


Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys 
225                 230                 235                 240 


Lys Ala Ser Gly Tyr Thr Phe Thr Gly Tyr Tyr Met His Trp Val Arg 
                245                 250                 255     


Gln Ala Pro Gly Gln Gly Leu Glu Trp Met Gly Arg Ile Asn Pro Asn 
            260                 265                 270         


Ser Gly Gly Thr Asn Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met 
        275                 280                 285             


Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu 
    290                 295                 300                 


Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys Ala Arg Val Pro Tyr Cys 
305                 310                 315                 320 


Ser Ser Thr Ser Cys His Arg Asp Trp Tyr Phe Asp Leu Trp Gly Arg 
                325                 330                 335     


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
            340                 345                 350         


Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Leu 
        355                 360                 365             


Ser Leu Pro Val Thr Pro Gly Glu Pro Ala Ser Ile Ser Cys Arg Ser 
    370                 375                 380                 


Ser Gln Ser Leu Leu Asp Ser Asp Asp Gly Asn Thr Tyr Leu Asp Trp 
385                 390                 395                 400 


Tyr Leu Gln Lys Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr Thr Leu 
                405                 410                 415     


Ser Tyr Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser 
            420                 425                 430         


Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val 
        435                 440                 445             


Gly Val Tyr Tyr Cys Met Gln Arg Ile Glu Phe Pro Leu Thr Phe Gly 
    450                 455                 460                 


Gly Gly Thr Lys Val Glu Ile Lys Ser Gly Gly Gly Ser His His His 
465                 470                 475                 480 


His His His 
            


