﻿                         序列表

<110>  南京北恒生物科技有限公司

<120>  靶向CD19的人源化抗体及其用途

<130>  BHPCT21

<160>  45

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  11
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CDR-L1


<400>  1
Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn 
1               5                   10      

<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CDR-L2


<400>  2
His Thr Ser Arg Leu His Ser 
1               5           

<210>  3
<211>  9
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CDR-L3


<400>  3
Gln Gln Gly Asn Thr Leu Pro Tyr Thr 
1               5                   

<210>  4
<211>  7
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CDR-H1


<400>  4
Gly Val Ser Leu Pro Asp Tyr 
1               5           

<210>  5
<211>  5
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CDR-H2


<400>  5
Trp Gly Ser Glu Thr 
1               5   

<210>  6
<211>  12
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CDR-H3


<400>  6
His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
1               5                   10          

<210>  7
<211>  107
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  fmc63 vL


<400>  7
Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          
Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              
Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  
Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      
Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
            100                 105         

<210>  8
<211>  120
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  fmc63 VH


<400>  8
Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
1               5                   10                  15      
Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
            20                  25                  30          
Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu 
        35                  40                  45              
Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  
Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  
Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala 
                85                  90                  95      
Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         
Gly Thr Ser Val Thr Val Ser Ser 
        115                 120 

<210>  9
<211>  242
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  fmc63 scFv


<400>  9
Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          
Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              
Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  
Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      
Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser 
            100                 105                 110         
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu 
        115                 120                 125             
Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys 
    130                 135                 140                 
Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg 
145                 150                 155                 160 
Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser 
                165                 170                 175     
Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile 
            180                 185                 190         
Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 
        195                 200                 205             
Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly 
    210                 215                 220                 
Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 
225                 230                 235                 240 
Ser Ser 
        

<210>  10
<211>  106
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  hVL-1


<400>  10
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Leu 
        35                  40                  45              
Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Gln 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
            100                 105     

<210>  11
<211>  120
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  hVH-1


<400>  11
Gln Val Thr Leu Lys Glu Ser Gly Pro Val Leu Val Lys Pro Thr Glu 
1               5                   10                  15      
Thr Leu Thr Leu Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
            20                  25                  30          
Gly Val Ser Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu Trp Leu 
        35                  40                  45              
Ala Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  
Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Ser Gln Val Val Leu 
65                  70                  75                  80  
Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala 
                85                  90                  95      
Arg His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         
Gly Thr Met Val Thr Val Ser Ser 
        115                 120 

<210>  12
<211>  106
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  hVL-2


<400>  12
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile 
            100                 105     

<210>  13
<211>  120
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  hVH-2


<400>  13
Gln Val Thr Leu Lys Glu Ser Gly Pro Val Leu Val Lys Pro Thr Glu 
1               5                   10                  15      
Thr Leu Thr Leu Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
            20                  25                  30          
Gly Val Ser Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu Trp Leu 
        35                  40                  45              
Ala Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  
Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Ser Gln Val Val Leu 
65                  70                  75                  80  
Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala 
                85                  90                  95      
Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         
Gly Thr Met Val Thr Val Ser Ser 
        115                 120 

<210>  14
<211>  241
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  hCD19-1


<400>  14
Gln Val Thr Leu Lys Glu Ser Gly Pro Val Leu Val Lys Pro Thr Glu 
1               5                   10                  15      
Thr Leu Thr Leu Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
            20                  25                  30          
Gly Val Ser Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu Trp Leu 
        35                  40                  45              
Ala Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  
Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Ser Gln Val Val Leu 
65                  70                  75                  80  
Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala 
                85                  90                  95      
Arg His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         
Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             
Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser 
    130                 135                 140                 
Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala 
145                 150                 155                 160 
Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly 
                165                 170                 175     
Lys Ala Pro Lys Leu Leu Leu Tyr His Thr Ser Arg Leu His Ser Gly 
            180                 185                 190         
Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu 
        195                 200                 205             
Thr Ile Ser Ser Leu Gln Gln Glu Asp Phe Ala Thr Tyr Tyr Cys Gln 
    210                 215                 220                 
Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu 
225                 230                 235                 240 
Ile 
    

<210>  15
<211>  241
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  hCD19-2


<400>  15
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Leu 
        35                  40                  45              
Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Gln 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Gly Gly Gly Gly Ser Gly 
            100                 105                 110         
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Thr Leu Lys Glu Ser 
        115                 120                 125             
Gly Pro Val Leu Val Lys Pro Thr Glu Thr Leu Thr Leu Thr Cys Thr 
    130                 135                 140                 
Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln 
145                 150                 155                 160 
Pro Pro Gly Lys Ala Leu Glu Trp Leu Ala Val Ile Trp Gly Ser Glu 
                165                 170                 175     
Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ser Lys 
            180                 185                 190         
Asp Thr Ser Lys Ser Gln Val Val Leu Thr Met Thr Asn Met Asp Pro 
        195                 200                 205             
Val Asp Thr Ala Thr Tyr Tyr Cys Ala Arg His Tyr Tyr Tyr Gly Gly 
    210                 215                 220                 
Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Met Val Thr Val Ser 
225                 230                 235                 240 
Ser 
    

<210>  16
<211>  241
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  hCD19-3


<400>  16
Gln Val Thr Leu Lys Glu Ser Gly Pro Val Leu Val Lys Pro Thr Glu 
1               5                   10                  15      
Thr Leu Thr Leu Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
            20                  25                  30          
Gly Val Ser Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu Trp Leu 
        35                  40                  45              
Ala Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  
Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Ser Gln Val Val Leu 
65                  70                  75                  80  
Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala 
                85                  90                  95      
Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         
Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             
Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser 
    130                 135                 140                 
Ser Val Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala 
145                 150                 155                 160 
Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly 
                165                 170                 175     
Lys Ala Pro Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly 
            180                 185                 190         
Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             
Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln 
    210                 215                 220                 
Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gln Gly Thr Arg Leu Glu 
225                 230                 235                 240 
Ile 
    

<210>  17
<211>  241
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  hCD19-4


<400>  17
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Gly Gly Gly Gly Ser Gly 
            100                 105                 110         
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Thr Leu Lys Glu Ser 
        115                 120                 125             
Gly Pro Val Leu Val Lys Pro Thr Glu Thr Leu Thr Leu Thr Cys Thr 
    130                 135                 140                 
Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln 
145                 150                 155                 160 
Pro Pro Gly Lys Ala Leu Glu Trp Leu Ala Val Ile Trp Gly Ser Glu 
                165                 170                 175     
Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ser Lys 
            180                 185                 190         
Asp Thr Ser Lys Ser Gln Val Val Leu Thr Met Thr Asn Met Asp Pro 
        195                 200                 205             
Val Asp Thr Ala Thr Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly 
    210                 215                 220                 
Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Met Val Thr Val Ser 
225                 230                 235                 240 
Ser 
    

<210>  18
<211>  723
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  hCD19-1 scFv


<400>  18
caggtaacct tgaaagaatc aggcccagtc ctggtaaagc cgacggagac attgacgctg        60
acctgcacag tcagtggagt gtcattgccc gattatggtg tctcttggat tcgccagcct       120
cctggcaagg ctcttgaatg gttggctgtc atctggggca gtgagactac ttattataac       180
agtgcgttga aatcccggct gactattagt aaagatactt ccaaaagcca agtagtcctc       240
actatgacaa acatggatcc tgtagacacg gctacttatt attgtgcacg gcattactac       300
tacggcggct cttatgctat ggattactgg ggtcaaggaa caatggttac tgtttcatct       360
ggcggcgggg ggtcaggtgg gggtggttcc gggggtggtg gtagtgacat ccaaatgaca       420
caatctccga gtagtctctc cgcaagtgtg ggcgatcgag ttacaattac ttgtcgagcc       480
tcacaggaca tatctaagta cttgaattgg taccaacaga agccggggaa ggcaccaaag       540
ttgctgctgt atcacactag tagattgcat agtggggtgc cctcaagatt cagtggaagt       600
ggatcaggca ctgattacac tttgactatt agtagtctgc aacaggaaga cttcgcaacg       660
tactattgtc agcaaggtaa caccttgccg tacacattcg gtcaaggtac caaagtggaa       720
att                                                                     723

<210>  19
<211>  723
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  hCD19-2 scFv


<400>  19
gatattcaga tgacccaaag tccgtctagc ttgtcagcct ccgtagggga cagagttacg        60
ataacttgta gagcttcaca ggatatatca aagtacctta attggtatca acaaaagcct       120
ggtaaagccc cgaagcttct cctttaccat acgtctcgac tccactccgg tgtcccttct       180
cgcttcagtg gttctgggag tgggaccgac tatacattga ccatctcttc tttgcaacaa       240
gaggacttcg ctacgtatta ctgtcagcaa ggtaatactc ttccgtatac tttcggacaa       300
ggcacgaaag tagaaattgg tggtggcggt tcaggcgggg gtggaagcgg ggggggtggg       360
tctcaggtca ccttgaagga atctggacct gttctggtga agcctacaga aactttgaca       420
ctgacatgca ccgtctctgg tgtatcattg ccggactacg gtgtgtcatg gattaggcaa       480
ccgcctggaa aagccttgga atggctcgcc gtcatctggg ggtctgagac gacctattac       540
aactcagcgt tgaagtcacg cctcacgatt tcaaaggaca cgtctaaatc acaagtggtt       600
ttgacaatga ctaacatgga cccggtcgat acggctactt actactgcgc tcgccactat       660
tattatgggg ggtcctatgc gatggactat tggggacagg gcacaatggt gacagtctca       720
agc                                                                     723

<210>  20
<211>  723
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  hCD19-3 scFv


<400>  20
caggtgacat tgaaagagtc cggacctgtt ctcgtcaaac cgacggagac gctcaccctt        60
acatgcacgg tcagtggcgt ctcactgcca gattatgggg tttcttggat aagacagccc       120
cctggcaagg cccttgaatg gcttgcggtc atctggggtt ctgaaacaac atattataac       180
agtgcactca aatcacgcct tacgatttca aaggatacaa gtaaatctca agttgttctg       240
accatgacga atatggatcc agtggacacc gcgacgtact attgcgcgaa acattactac       300
tacggcggaa gctacgctat ggactattgg ggacaaggaa cgatggtcac tgtctctagc       360
ggcgggggtg gaagtggagg agggggatca gggggaggag gaagtgacat tcaaatgacg       420
cagtctccta gttcagtctc tgcatctgtg ggcgacagag tcacgattac ctgtagggca       480
agccaggata tctctaagta ccttaattgg tatcaacaaa aaccagggaa ggccccgaag       540
cttcttatct accatacaag ccgactccat agtggggttc cctcacgatt tagcgggtct       600
ggctctggta cagacttcac tctcactatt agtagcctgc aaccagagga cttcgcaacc       660
tactactgcc agcagggaaa cactcttcca tatacattcg gacagggaac ccggctcgag       720
att                                                                     723

<210>  21
<211>  723
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  hCD19-4 scFv


<400>  21
gatatacaaa tgacgcagtc tccatcatcc gtatccgcca gcgtaggaga tagagtcacc        60
atcacatgtc gcgcgagtca ggacatcagc aaatacctca attggtacca gcaaaagccc       120
ggcaaggctc caaaattgct gatataccat acgagtaggt tgcacagtgg agtacccagt       180
agattttctg ggtcagggag tggcacagat ttcacgctga caataagtag tcttcaacct       240
gaagatttcg caacctatta ctgtcaacaa ggtaatacgc tgccgtacac gtttggtcag       300
ggtacgaggc tcgaaatcgg tggcggggga agtggaggtg gtggatctgg gggtgggggc       360
tctcaagtta ctttgaaaga gagtggacca gtgctcgtaa agcctacgga gacgcttacc       420
cttacgtgca cagtatctgg ggtgagtctc ccagattacg gtgtaagttg gatacgccag       480
ccgccgggga aagccttgga atggctcgca gtgatttggg gatccgaaac tacctattac       540
aatagcgccc ttaaatccag gctgactatt tccaaagaca cgagcaaatc ccaggtcgtc       600
cttaccatga caaacatgga cccagttgac accgctacct actactgtgc gaaacattat       660
tactacggtg ggagctatgc tatggactac tggggccaag gcacaatggt cactgttagt       720
agc                                                                     723

<210>  22
<211>  18
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  接头


<400>  22
Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      
Lys Gly 
        

<210>  23
<211>  15
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  接头


<400>  23
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  

<210>  24
<211>  25
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD8α跨膜结构域


<400>  24
Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      
Ser Leu Val Ile Thr Leu Tyr Cys Lys 
            20                  25  

<210>  25
<211>  75
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD8α跨膜结构域


<400>  25
atctacatct gggcgccctt ggccgggact tgtggggtcc ttctcctgtc actggttatc        60
accctttact gcaaa                                                         75

<210>  26
<211>  27
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD28跨膜结构域


<400>  26
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          

<210>  27
<211>  81
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD28跨膜结构域


<400>  27
ttttgggtcc tcgtcgtagt tggaggggta cttgcctgtt atagcctcct ggttaccgta        60
gcatttatta tattctgggt g                                                  81

<210>  28
<211>  41
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD28共刺激结构域


<400>  28
Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      

<210>  29
<211>  123
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD28共刺激结构域


<400>  29
aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgcccc        60
gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc       120
tcc                                                                     123

<210>  30
<211>  40
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  4-1BB共刺激结构域


<400>  30
Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
1               5                   10                  15      
Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
            20                  25                  30          
Glu Glu Glu Glu Gly Gly Cys Glu 
        35                  40  

<210>  31
<211>  120
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  4-1BB共刺激结构域


<400>  31
cggggcagaa agaaactcct gtatatattc aaacaaccat ttatgagacc agtacaaact        60
actcaagagg aagatggctg tagctgccga tttccagaag aagaagaagg aggatgtgaa       120

<210>  32
<211>  113
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD3ζ信号传导结构域


<400>  32
Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
1               5                   10                  15      
Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
            20                  25                  30          
Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
        35                  40                  45              
Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  
Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  
Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      
Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            100                 105                 110         
Arg 
    

<210>  33
<211>  339
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD3ζ信号传导结构域



<400>  33
ctgagagtga agttcagcag gagcgcagac gcccccgcgt accagcaggg ccagaaccag        60
ctctataacg agctcaatct aggacgaaga gaggagtacg atgttttgga caagagacgt       120
ggccgggacc ctgagatggg gggaaagccg agaaggaaga accctcagga aggcctgtac       180
aatgaactgc agaaagataa gatggcggag gcctacagtg agattgggat gaaaggcgag       240
cgccggaggg gcaaggggca cgatggcctt taccagggtc tcagtacagc caccaaggac       300
acctacgacg cccttcacat gcaggccctg ccccctcgc                              339

<210>  34
<211>  114
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD3ζ信号传导结构域


<400>  34
Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
1               5                   10                  15      
Gly Gln Asn Gln Leu Phe Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
            20                  25                  30          
Phe Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
        35                  40                  45              
Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
    50                  55                  60                  
Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
65                  70                  75                  80  
Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Phe Gln Gly Leu Ser 
                85                  90                  95      
Thr Ala Thr Lys Asp Thr Phe Asp Ala Leu His Met Gln Ala Leu Pro 
            100                 105                 110         
Pro Arg 
        

<210>  35
<211>  342
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD3ζ信号传导结构域


<400>  35
ctgagagtga agttcagcag gagcgcagac gcccccgcgt accagcaggg ccagaaccag        60
ctctttaacg agctcaatct aggacgaaga gaggagttcg atgttttgga caagagacgt       120
ggccgggacc ctgagatggg gggaaagccg cagagaagga agaaccctca ggaaggcctg       180
tacaatgaac tgcagaaaga taagatggcg gaggcctaca gtgagattgg gatgaaaggc       240
gagcgccgga ggggcaaggg gcacgatggc cttttccagg gtctcagtac agccaccaag       300
gacacctttg acgcccttca catgcaggcc ctgccccctc gc                          342

<210>  36
<211>  20
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  B2M信号肽


<400>  36
Met Ser Arg Ser Val Ala Leu Ala Val Leu Ala Leu Leu Ser Leu Ser 
1               5                   10                  15      
Gly Leu Glu Ala 
            20  

<210>  37
<211>  60
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  B2M信号肽


<400>  37
atgtcccgct ctgttgcttt ggctgtgctg gcccttttgt cccttagcgg actggaggcc        60

<210>  38
<211>  21
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD8α信号肽


<400>  38
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      
His Ala Ala Arg Pro 
            20      

<210>  39
<211>  63
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD8α信号肽


<400>  39
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg        60
ccg                                                                      63

<210>  40
<211>  45
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD8α铰链区


<400>  40
Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      
Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          
Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  

<210>  41
<211>  135
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD8α铰链区


<400>  41
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg        60
tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg       120
gacttcgcct gtgat                                                        135

<210>  42
<211>  39
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD28铰链区


<400>  42
Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
1               5                   10                  15      
Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
            20                  25                  30          
Phe Pro Gly Pro Ser Lys Pro 
        35                  

<210>  43
<211>  117
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  CD28铰链区


<400>  43
attgaagtta tgtatcctcc tccttaccta gacaatgaga agagcaatgg aaccattatc        60
catgtgaaag ggaaacacct ttgtccaagt cccctatttc ccggaccttc taagccc          117

<210>  44
<211>  12
<212>  PRT
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  IgG4铰链区



<400>  44
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10          

<210>  45
<211>  36
<212>  DNA
<213>  Artificial Sequence(Artificial Sequence)

<220>
<223>  IgG4铰链区


<400>  45
gaaagcaaat acgggccgcc gtgtccaccc tgtccg                                  36

