﻿               SEQUENCE LISTING

<110> IMBA – Institut für Molekulare Biotechnologie GmbH

<120> Superior neutralization of SARS-CoV-2 by deglycosylated human angiotensin converting enzyme 2

<130> 12961 P 7930 WO

<160> 12

<170> BiSSAP 1.3.6

<210> 1
<211> 740
<212> PRT
<213> Homo sapiens


<220> 
<221> SIGNAL
<222> 1..17
<223> Signal sequence of ACE2

<220> 
<223> Truncated human ACE2 (amino acids 1-740)

<220> 
<221> DOMAIN
<222> 19..740
<223> Extracellular domain of ACE2

<220> 
<221> CARBOHYD
<222> 53
<223> Potential glycosylation site

<220> 
<221> CARBOHYD
<222> 90
<223> Potential glycosylation site

<220> 
<221> CARBOHYD
<222> 103
<223> Potential glycosylation site

<220> 
<221> CARBOHYD
<222> 322
<223> Potential glycosylation site

<220> 
<221> CARBOHYD
<222> 432
<223> Potential glycosylation site

<220> 
<221> CARBOHYD
<222> 546
<223> Potential glycosylation site

<220> 
<221> CARBOHYD
<222> 690
<223> Potential glycosylation site

<400> 1
Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala 
1               5                   10                  15      
Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe 
            20                  25                  30          
Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp 
        35                  40                  45              
Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn 
    50                  55                  60                  
Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala 
65                  70                  75                  80  
Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln 
                85                  90                  95      
Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys 
            100                 105                 110         
Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 
        115                 120                 125             
Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu 
    130                 135                 140                 
Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu 
145                 150                 155                 160 
Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu 
                165                 170                 175     
Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 
            180                 185                 190         
Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 
        195                 200                 205             
Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu 
    210                 215                 220                 
Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu 
225                 230                 235                 240 
His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile 
                245                 250                 255     
Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 
            260                 265                 270         
Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys 
        275                 280                 285             
Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala 
    290                 295                 300                 
Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 
305                 310                 315                 320 
Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro 
                325                 330                 335     
Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly 
            340                 345                 350         
Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp 
        355                 360                 365             
Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 
    370                 375                 380                 
Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 
385                 390                 395                 400 
His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 
                405                 410                 415     
His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn 
            420                 425                 430         
Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 
        435                 440                 445             
Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 
    450                 455                 460                 
Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met 
465                 470                 475                 480 
Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr 
                485                 490                 495     
Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 
            500                 505                 510         
Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala 
        515                 520                 525             
Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile 
    530                 535                 540                 
Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu 
545                 550                 555                 560 
Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala 
                565                 570                 575     
Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe 
            580                 585                 590         
Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr 
        595                 600                 605             
Asp Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu 
    610                 615                 620                 
Lys Ser Ala Leu Gly Asp Lys Ala Tyr Glu Trp Asn Asp Asn Glu Met 
625                 630                 635                 640 
Tyr Leu Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu 
                645                 650                 655     
Lys Val Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val 
            660                 665                 670         
Ala Asn Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro 
        675                 680                 685             
Lys Asn Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile 
    690                 695                 700                 
Arg Met Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn 
705                 710                 715                 720 
Ser Leu Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln 
                725                 730                 735     
Pro Pro Val Ser 
            740 

<210> 2
<211> 805
<212> PRT
<213> Homo sapiens


<220> 
<221> SIGNAL
<222> 1..17
<223> Signal sequence of ACE2

<220> 
<223> Full-length human ACE2

<220> 
<221> DOMAIN
<222> 19..740
<223> Extracellular domain of ACE2

<220> 
<221> CARBOHYD
<222> 53
<223> Potential glycosylation site

<220> 
<221> CARBOHYD
<222> 90
<223> Potential glycosylation site

<220> 
<221> CARBOHYD
<222> 103
<223> Potential glycosylation site

<220> 
<221> CARBOHYD
<222> 322
<223> Potential glycosylation site

<220> 
<221> CARBOHYD
<222> 432
<223> Potential glycosylation site

<220> 
<221> CARBOHYD
<222> 546
<223> Potential glycosylation site

<220> 
<221> CARBOHYD
<222> 690
<223> Potential glycosylation site

<220> 
<221> DOMAIN
<222> 741..761
<223> Transmembrane domain of ACE2

<220> 
<221> DOMAIN
<222> 762..805
<223> Cytoplasmic domain of ACE2

<400> 2
Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala 
1               5                   10                  15      
Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe 
            20                  25                  30          
Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp 
        35                  40                  45              
Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn 
    50                  55                  60                  
Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala 
65                  70                  75                  80  
Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln 
                85                  90                  95      
Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys 
            100                 105                 110         
Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 
        115                 120                 125             
Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu 
    130                 135                 140                 
Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu 
145                 150                 155                 160 
Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu 
                165                 170                 175     
Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 
            180                 185                 190         
Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 
        195                 200                 205             
Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu 
    210                 215                 220                 
Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu 
225                 230                 235                 240 
His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile 
                245                 250                 255     
Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 
            260                 265                 270         
Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys 
        275                 280                 285             
Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala 
    290                 295                 300                 
Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 
305                 310                 315                 320 
Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro 
                325                 330                 335     
Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly 
            340                 345                 350         
Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp 
        355                 360                 365             
Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 
    370                 375                 380                 
Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 
385                 390                 395                 400 
His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 
                405                 410                 415     
His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn 
            420                 425                 430         
Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 
        435                 440                 445             
Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 
    450                 455                 460                 
Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met 
465                 470                 475                 480 
Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr 
                485                 490                 495     
Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 
            500                 505                 510         
Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala 
        515                 520                 525             
Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile 
    530                 535                 540                 
Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu 
545                 550                 555                 560 
Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala 
                565                 570                 575     
Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe 
            580                 585                 590         
Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr 
        595                 600                 605             
Asp Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu 
    610                 615                 620                 
Lys Ser Ala Leu Gly Asp Lys Ala Tyr Glu Trp Asn Asp Asn Glu Met 
625                 630                 635                 640 
Tyr Leu Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu 
                645                 650                 655     
Lys Val Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val 
            660                 665                 670         
Ala Asn Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro 
        675                 680                 685             
Lys Asn Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile 
    690                 695                 700                 
Arg Met Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn 
705                 710                 715                 720 
Ser Leu Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln 
                725                 730                 735     
Pro Pro Val Ser Ile Trp Leu Ile Val Phe Gly Val Val Met Gly Val 
            740                 745                 750         
Ile Val Val Gly Ile Val Ile Leu Ile Phe Thr Gly Ile Arg Asp Arg 
        755                 760                 765             
Lys Lys Lys Asn Lys Ala Arg Ser Gly Glu Asn Pro Tyr Ala Ser Ile 
    770                 775                 780                 
Asp Ile Ser Lys Gly Glu Asn Asn Pro Gly Phe Gln Asn Thr Asp Asp 
785                 790                 795                 800 
Val Gln Thr Ser Phe 
                805 

<210> 3
<211> 14
<212> PRT
<213> Homo sapiens


<220> 
<223> Sequence 3

<220> 
<221> CARBOHYD
<222> 5
<223> Potential glycosylation site N53

<400> 3
Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met 
1               5                   10                  

<210> 4
<211> 12
<212> PRT
<213> Homo sapiens


<220> 
<223> Sequence 4

<220> 
<221> CARBOHYD
<222> 11
<223> Potential glycosylation site N90

<400> 4
Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu 
1               5                   10          

<210> 5
<211> 13
<212> PRT
<213> Homo sapiens


<220> 
<223> Sequence 5

<220> 
<221> CARBOHYD
<222> 8
<223> Potential glycosylation site N103

<400> 5
Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu 
1               5                   10              

<210> 6
<211> 13
<212> PRT
<213> Homo sapiens


<220> 
<223> Sequence 6

<220> 
<221> CARBOHYD
<222> 7
<223> Potential glycosylation site N322

<400> 6
Val Ser Val Gly Leu Pro Asn Met Thr Gln Gly Phe Trp 
1               5                   10              

<210> 7
<211> 15
<212> PRT
<213> Homo sapiens


<220> 
<223> Sequence 7

<220> 
<221> CARBOHYD
<222> 9
<223> Potential glycosylation site N432

<400> 7
Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu Thr Glu Ile Asn Phe 
1               5                   10                  15  

<210> 8
<211> 14
<212> PRT
<213> Homo sapiens


<220> 
<223> Sequence 8

<220> 
<221> CARBOHYD
<222> 8
<223> Potential glycolysation site N432

<400> 8
Ser Pro Asp Phe Gln Glu Asp Asn Glu Thr Glu Ile Asn Phe 
1               5                   10                  

<210> 9
<211> 12
<212> PRT
<213> Homo sapiens


<220> 
<223> Sequence 9

<220> 
<221> CARBOHYD
<222> 5
<223> Potential glycosylation site N546

<400> 9
Cys Asp Ile Ser Asn Ser Thr Glu Ala Gly Gln Lys 
1               5                   10          

<210> 10
<211> 8
<212> PRT
<213> Homo sapiens


<220> 
<221> CARBOHYD
<222> 1
<223> Potential glycosylation site N690

<220> 
<223> Sequence 10

<400> 10
Asn Val Ser Asp Ile Ile Pro Arg 
1               5               

<210> 11
<211> 16
<212> PRT
<213> Homo sapiens


<220> 
<223> Sequence 11

<220> 
<221> CARBOHYD
<222> 6
<223> Potential glycosylation site T730

<400> 11
Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln Pro Pro Val Ser 
1               5                   10                  15      



<210> 12
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> Sequence 12

<220> 
<221> CARBOHYD
<222> 5
<223> Potential glycosylation site N694

<400> 12
Glu Glu Gln Tyr Asn Ser Thr Tyr 
1               5               

