               SEQUENCE LISTING

<110> Nordic Bioscience A/S

<120> Assay for detecting Collagen XVIII biomarkers

<130> P21209WO

<150> GB2019578.0
<151> 2020-12-11

<160> 20

<170> BiSSAP 1.3.6

<210> 1
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 1
Asn Leu Leu Asn Leu Asn 
1               5       

<210> 2
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 2
Asn Leu Leu Asn Leu Asn Lys Asp Lys Glu 
1               5                   10  

<210> 3
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 3
Asp Tyr Trp Ile His 
1               5   

<210> 4
<211> 17
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 4
Glu Ile Asn Pro Ser Asp Gly Arg Ser Asn Tyr Asn Glu Lys Phe Lys 
1               5                   10                  15      
Thr 
    

<210> 5
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 5
Asp Leu Gly Phe Ala Asp 
1               5       

<210> 6
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 6
Arg Ser Ser Gln Ser Ile Val Tyr Ser Asn Gly Asn Thr Tyr Leu Gln 
1               5                   10                  15      



<210> 7
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 7
Lys Val Ser Asn Arg Phe Ser 
1               5           

<210> 8
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 8
Phe Gln Gly Ser His Val Pro Phe Thr 
1               5                   

<210> 9
<211> 89
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 9
Arg Ser Ser Gln Ser Ile Val Tyr Ser Asn Gly Asn Thr Tyr Leu Gln 
1               5                   10                  15      
Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr Lys 
            20                  25                  30          
Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly 
        35                  40                  45              
Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp 
    50                  55                  60                  
Val Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Phe Thr Phe 
65                  70                  75                  80  
Gly Ser Gly Thr Lys Leu Glu Ile Lys 
                85                  

<210> 10
<211> 85
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 10
Asp Tyr Trp Ile His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 
1               5                   10                  15      
Trp Ile Gly Glu Ile Asn Pro Ser Asp Gly Arg Ser Asn Tyr Asn Glu 
            20                  25                  30          
Lys Phe Lys Thr Lys Ala Thr Leu Thr Val Asp Arg Ser Ser Ser Thr 
        35                  40                  45              
Ala Tyr Met His Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Phe 
    50                  55                  60                  
Tyr Cys Ala Arg Asp Leu Gly Phe Ala Asp Trp Gly Pro Gly Thr Thr 
65                  70                  75                  80  
Val Thr Val Ser Ser 
                85  

<210> 11
<211> 112
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 11
Asp Ile Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val Gly Leu Gly 
1               5                   10                  15      
Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val Tyr Ser 
            20                  25                  30          
Asn Gly Asn Thr Tyr Leu Gln Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              
Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      
Ser His Val Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         



<210> 12
<211> 115
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 12
Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      
Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ile Asp Tyr 
            20                  25                  30          
Trp Ile His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              
Gly Glu Ile Asn Pro Ser Asp Gly Arg Ser Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  
Lys Thr Lys Ala Thr Leu Thr Val Asp Arg Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  
Met His Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Phe Tyr Cys 
                85                  90                  95      
Ala Arg Asp Leu Gly Phe Ala Asp Trp Gly Pro Gly Thr Thr Val Thr 
            100                 105                 110         
Val Ser Ser 
        115 

<210> 13
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<220> 
<221> MOD_RES
<222> 10
<223> Amino acid is biotinylated via an optional linker

<400> 13
Asn Leu Leu Asn Leu Asn Lys Asp Lys Glu 
1               5                   10  

<210> 14
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 14
Asn Leu Leu Asn Leu Asn Trp Leu Trp Phe 
1               5                   10  

<210> 15
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<220> 
<221> MOD_RES
<222> 11
<223> Biotinylated

<400> 15
Asn Leu Leu Asn Leu Asn Lys Asp Lys Glu Lys 
1               5                   10      

<210> 16
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<220> 
<221> MOD_RES
<222> 15
<223> Bound to keyhole limpet hemocyanin

<400> 16
Asn Leu Leu Asn Leu Asn Lys Asp Lys Glu Lys Lys Gly Gly Cys 
1               5                   10                  15  

<210> 17
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 17
Leu His Asp Ser Asn Pro Tyr Pro Arg Arg 
1               5                   10  

<210> 18
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 18
Asn Ala Leu Asn Leu Asn Trp Leu Trp Phe 
1               5                   10  

<210> 19
<211> 514
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 19
Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      
Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ile Asp Tyr 
            20                  25                  30          
Trp Ile His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              
Gly Glu Ile Asn Pro Ser Asp Gly Arg Ser Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  
Lys Thr Lys Ala Thr Leu Thr Val Asp Arg Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  
Met His Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Phe Tyr Cys 
                85                  90                  95      
Ala Arg Asp Leu Gly Phe Ala Asp Trp Gly Pro Gly Thr Thr Val Thr 
            100                 105                 110         
Val Ser Ser Ala Lys Thr Thr Ala Pro Ser Val Tyr Pro Leu Ala Pro 
        115                 120                 125             
Val Cys Gly Asp Thr Thr Gly Ser Ser Val Thr Leu Gly Cys Leu Val 
    130                 135                 140                 
Lys Gly Tyr Phe Pro Glu Pro Val Thr Leu Thr Trp Asn Ser Gly Ser 
145                 150                 155                 160 
Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu 
                165                 170                 175     
Tyr Thr Leu Ser Ser Ser Val Thr Val Thr Ser Ser Thr Trp Pro Ser 
            180                 185                 190         
Gln Ser Ile Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val 
        195                 200                 205             
Asp Lys Lys Ile Glu Pro Arg Gly Pro Thr Ile Lys Pro Cys Pro Pro 
    210                 215                 220                 
Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly Gly Pro Ser Val Phe Ile 
225                 230                 235                 240 
Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu Ser Pro Ile 
                245                 250                 255     
Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro Asp Val Gln 
            260                 265                 270         
Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala Gln Thr Gln 
        275                 280                 285             
Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg Val Val Ser Ala Leu 
    290                 295                 300                 
Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys Cys Lys 
305                 310                 315                 320 
Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr Ile Ser Lys 
                325                 330                 335     
Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu Pro Pro Pro 
            340                 345                 350         
Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys Met Val Thr 
        355                 360                 365             
Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn Asn Gly Lys 
    370                 375                 380                 
Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 
Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys Asn Trp Val 
                405                 410                 415     
Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly Leu His Asn 
            420                 425                 430         
His His Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly Leu Asp Leu Asp 
        435                 440                 445             
Asp Val Cys Ala Glu Ala Gln Asp Gly Glu Leu Asp Gly Leu Trp Thr 
    450                 455                 460                 
Thr Ile Thr Ile Phe Ile Ser Leu Phe Leu Leu Ser Val Cys Tyr Ser 
465                 470                 475                 480 
Ala Ser Val Thr Leu Phe Lys Val Lys Trp Ile Phe Ser Ser Val Val 
                485                 490                 495     
Glu Leu Lys Gln Thr Ile Ser Pro Asp Tyr Arg Asn Met Ile Gly Gln 
            500                 505                 510         
Gly Ala 
        

<210> 20
<211> 219
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic peptide

<400> 20
Asp Ile Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val Gly Leu Gly 
1               5                   10                  15      
Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val Tyr Ser 
            20                  25                  30          
Asn Gly Asn Thr Tyr Leu Gln Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              
Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      
Ser His Val Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         
Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu 
        115                 120                 125             
Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe 
    130                 135                 140                 
Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg 
145                 150                 155                 160 
Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser 
                165                 170                 175     
Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu 
            180                 185                 190         
Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser 
        195                 200                 205             
Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
    210                 215                 

