                         SEQUENCE LISTING

<110>  AstraZeneca AB
 
<120>  Lipocalin mutein dry powder formulation for treatment of asthma

<130>  008136756 

<150>  US 63/127903
<151>  2020-12-18

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the human tear lipocalin mutein 
       PRS-060/AZD1402

<400>  1

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Ser Arg Cys Pro Arg Ala Val Tyr Asn Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Phe Thr Ala Gln Arg Lys Gly Arg Trp Gln Lys Tyr Lys Leu Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Ser Gly Gly Arg His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe His 
                85                  90                  95      


Ser Glu Gly Leu Cys Pro Gly Gln Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly Ser Asp 
145                 150                 


<210>  2
<211>  176
<212>  PRT
<213>  Homo sapiens tear lipocalin

<400>  2

Met Lys Pro Leu Leu Leu Ala Val Ser Leu Gly Leu Ile Ala Ala Leu 
1               5                   10                  15      


Gln Ala His His Leu Leu Ala Ser Asp Glu Glu Ile Gln Asp Val Ser 
            20                  25                  30          


Gly Thr Trp Tyr Leu Lys Ala Met Thr Val Asp Arg Glu Phe Pro Glu 
        35                  40                  45              


Met Asn Leu Glu Ser Val Thr Pro Met Thr Leu Thr Thr Leu Glu Gly 
    50                  55                  60                  


Gly Asn Leu Glu Ala Lys Val Thr Met Leu Ile Ser Gly Arg Cys Gln 
65                  70                  75                  80  


Glu Val Lys Ala Val Leu Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr 
                85                  90                  95      


Ala Asp Gly Gly Lys His Val Ala Tyr Ile Ile Arg Ser His Val Lys 
            100                 105                 110         


Asp His Tyr Ile Phe Tyr Cys Glu Gly Glu Leu His Gly Lys Pro Val 
        115                 120                 125             


Arg Gly Val Lys Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala 
    130                 135                 140                 


Leu Glu Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu 
145                 150                 155                 160 


Ser Ile Leu Ile Pro Arg Gln Ser Glu Thr Cys Ser Pro Gly Ser Asp 
                165                 170                 175     


<210>  3
<211>  158
<212>  PRT
<213>  Homo sapiens tear lipocalin mature form

<400>  3

His His Leu Leu Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr 
1               5                   10                  15      


Trp Tyr Leu Lys Ala Met Thr Val Asp Arg Glu Phe Pro Glu Met Asn 
            20                  25                  30          


Leu Glu Ser Val Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn 
        35                  40                  45              


Leu Glu Ala Lys Val Thr Met Leu Ile Ser Gly Arg Cys Gln Glu Val 
    50                  55                  60                  


Lys Ala Val Leu Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Asp 
65                  70                  75                  80  


Gly Gly Lys His Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His 
                85                  90                  95      


Tyr Ile Phe Tyr Cys Glu Gly Glu Leu His Gly Lys Pro Val Arg Gly 
            100                 105                 110         


Val Lys Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu 
        115                 120                 125             


Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile 
    130                 135                 140                 


Leu Ile Pro Arg Gln Ser Glu Thr Cys Ser Pro Gly Ser Asp 
145                 150                 155             


<210>  4
<211>  800
<212>  PRT
<213>  Homo sapiens interleukin-4 receptor alpha chain

<400>  4

Met Lys Val Leu Gln Glu Pro Thr Cys Val Ser Asp Tyr Met Ser Ile 
1               5                   10                  15      


Ser Thr Cys Glu Trp Lys Met Asn Gly Pro Thr Asn Cys Ser Thr Glu 
            20                  25                  30          


Leu Arg Leu Leu Tyr Gln Leu Val Phe Leu Leu Ser Glu Ala His Thr 
        35                  40                  45              


Cys Ile Pro Glu Asn Asn Gly Gly Ala Gly Cys Val Cys His Leu Leu 
    50                  55                  60                  


Met Asp Asp Val Val Ser Ala Asp Asn Tyr Thr Leu Asp Leu Trp Ala 
65                  70                  75                  80  


Gly Gln Gln Leu Leu Trp Lys Gly Ser Phe Lys Pro Ser Glu His Val 
                85                  90                  95      


Lys Pro Arg Ala Pro Gly Asn Leu Thr Val His Thr Asn Val Ser Asp 
            100                 105                 110         


Thr Leu Leu Leu Thr Trp Ser Asn Pro Tyr Pro Pro Asp Asn Tyr Leu 
        115                 120                 125             


Tyr Asn His Leu Thr Tyr Ala Val Asn Ile Trp Ser Glu Asn Asp Pro 
    130                 135                 140                 


Ala Asp Phe Arg Ile Tyr Asn Val Thr Tyr Leu Glu Pro Ser Leu Arg 
145                 150                 155                 160 


Ile Ala Ala Ser Thr Leu Lys Ser Gly Ile Ser Tyr Arg Ala Arg Val 
                165                 170                 175     


Arg Ala Trp Ala Gln Cys Tyr Asn Thr Thr Trp Ser Glu Trp Ser Pro 
            180                 185                 190         


Ser Thr Lys Trp His Asn Ser Tyr Arg Glu Pro Phe Glu Gln His Leu 
        195                 200                 205             


Leu Leu Gly Val Ser Val Ser Cys Ile Val Ile Leu Ala Val Cys Leu 
    210                 215                 220                 


Leu Cys Tyr Val Ser Ile Thr Lys Ile Lys Lys Glu Trp Trp Asp Gln 
225                 230                 235                 240 


Ile Pro Asn Pro Ala Arg Ser Arg Leu Val Ala Ile Ile Ile Gln Asp 
                245                 250                 255     


Ala Gln Gly Ser Gln Trp Glu Lys Arg Ser Arg Gly Gln Glu Pro Ala 
            260                 265                 270         


Lys Cys Pro His Trp Lys Asn Cys Leu Thr Lys Leu Leu Pro Cys Phe 
        275                 280                 285             


Leu Glu His Asn Met Lys Arg Asp Glu Asp Pro His Lys Ala Ala Lys 
    290                 295                 300                 


Glu Met Pro Phe Gln Gly Ser Gly Lys Ser Ala Trp Cys Pro Val Glu 
305                 310                 315                 320 


Ile Ser Lys Thr Val Leu Trp Pro Glu Ser Ile Ser Val Val Arg Cys 
                325                 330                 335     


Val Glu Leu Phe Glu Ala Pro Val Glu Cys Glu Glu Glu Glu Glu Val 
            340                 345                 350         


Glu Glu Glu Lys Gly Ser Phe Cys Ala Ser Pro Glu Ser Ser Arg Asp 
        355                 360                 365             


Asp Phe Gln Glu Gly Arg Glu Gly Ile Val Ala Arg Leu Thr Glu Ser 
    370                 375                 380                 


Leu Phe Leu Asp Leu Leu Gly Glu Glu Asn Gly Gly Phe Cys Gln Gln 
385                 390                 395                 400 


Asp Met Gly Glu Ser Cys Leu Leu Pro Pro Ser Gly Ser Thr Ser Ala 
                405                 410                 415     


His Met Pro Trp Asp Glu Phe Pro Ser Ala Gly Pro Lys Glu Ala Pro 
            420                 425                 430         


Pro Trp Gly Lys Glu Gln Pro Leu His Leu Glu Pro Ser Pro Pro Ala 
        435                 440                 445             


Ser Pro Thr Gln Ser Pro Asp Asn Leu Thr Cys Thr Glu Thr Pro Leu 
    450                 455                 460                 


Val Ile Ala Gly Asn Pro Ala Tyr Arg Ser Phe Ser Asn Ser Leu Ser 
465                 470                 475                 480 


Gln Ser Pro Cys Pro Arg Glu Leu Gly Pro Asp Pro Leu Leu Ala Arg 
                485                 490                 495     


His Leu Glu Glu Val Glu Pro Glu Met Pro Cys Val Pro Gln Leu Ser 
            500                 505                 510         


Glu Pro Thr Thr Val Pro Gln Pro Glu Pro Glu Thr Trp Glu Gln Ile 
        515                 520                 525             


Leu Arg Arg Asn Val Leu Gln His Gly Ala Ala Ala Ala Pro Val Ser 
    530                 535                 540                 


Ala Pro Thr Ser Gly Tyr Gln Glu Phe Val His Ala Val Glu Gln Gly 
545                 550                 555                 560 


Gly Thr Gln Ala Ser Ala Val Val Gly Leu Gly Pro Pro Gly Glu Ala 
                565                 570                 575     


Gly Tyr Lys Ala Phe Ser Ser Leu Leu Ala Ser Ser Ala Val Ser Pro 
            580                 585                 590         


Glu Lys Cys Gly Phe Gly Ala Ser Ser Gly Glu Glu Gly Tyr Lys Pro 
        595                 600                 605             


Phe Gln Asp Leu Ile Pro Gly Cys Pro Gly Asp Pro Ala Pro Val Pro 
    610                 615                 620                 


Val Pro Leu Phe Thr Phe Gly Leu Asp Arg Glu Pro Pro Arg Ser Pro 
625                 630                 635                 640 


Gln Ser Ser His Leu Pro Ser Ser Ser Pro Glu His Leu Gly Leu Glu 
                645                 650                 655     


Pro Gly Glu Lys Val Glu Asp Met Pro Lys Pro Pro Leu Pro Gln Glu 
            660                 665                 670         


Gln Ala Thr Asp Pro Leu Val Asp Ser Leu Gly Ser Gly Ile Val Tyr 
        675                 680                 685             


Ser Ala Leu Thr Cys His Leu Cys Gly His Leu Lys Gln Cys His Gly 
    690                 695                 700                 


Gln Glu Asp Gly Gly Gln Thr Pro Val Met Ala Ser Pro Cys Cys Gly 
705                 710                 715                 720 


Cys Cys Cys Gly Asp Arg Ser Ser Pro Pro Thr Thr Pro Leu Arg Ala 
                725                 730                 735     


Pro Asp Pro Ser Pro Gly Gly Val Pro Leu Glu Ala Ser Leu Cys Pro 
            740                 745                 750         


Ala Ser Leu Ala Pro Ser Gly Ile Ser Glu Lys Ser Lys Ser Ser Ser 
        755                 760                 765             


Ser Phe His Pro Ala Pro Gly Asn Ala Gln Ser Ser Ser Gln Thr Pro 
    770                 775                 780                 


Lys Ile Val Asn Phe Val Ser Val Gly Pro Thr Tyr Met Arg Val Ser 
785                 790                 795                 800 


<210>  5
<211>  207
<212>  PRT
<213>  Homo sapiens extracellular domain of interleukin-4 receptor alpha chain

<400>  5

Met Lys Val Leu Gln Glu Pro Thr Cys Val Ser Asp Tyr Met Ser Ile 
1               5                   10                  15      


Ser Thr Cys Glu Trp Lys Met Asn Gly Pro Thr Asn Cys Ser Thr Glu 
            20                  25                  30          


Leu Arg Leu Leu Tyr Gln Leu Val Phe Leu Leu Ser Glu Ala His Thr 
        35                  40                  45              


Cys Ile Pro Glu Asn Asn Gly Gly Ala Gly Cys Val Cys His Leu Leu 
    50                  55                  60                  


Met Asp Asp Val Val Ser Ala Asp Asn Tyr Thr Leu Asp Leu Trp Ala 
65                  70                  75                  80  


Gly Gln Gln Leu Leu Trp Lys Gly Ser Phe Lys Pro Ser Glu His Val 
                85                  90                  95      


Lys Pro Arg Ala Pro Gly Asn Leu Thr Val His Thr Asn Val Ser Asp 
            100                 105                 110         


Thr Leu Leu Leu Thr Trp Ser Asn Pro Tyr Pro Pro Asp Asn Tyr Leu 
        115                 120                 125             


Tyr Asn His Leu Thr Tyr Ala Val Asn Ile Trp Ser Glu Asn Asp Pro 
    130                 135                 140                 


Ala Asp Phe Arg Ile Tyr Asn Val Thr Tyr Leu Glu Pro Ser Leu Arg 
145                 150                 155                 160 


Ile Ala Ala Ser Thr Leu Lys Ser Gly Ile Ser Tyr Arg Ala Arg Val 
                165                 170                 175     


Arg Ala Trp Ala Gln Cys Tyr Asn Thr Thr Trp Ser Glu Trp Ser Pro 
            180                 185                 190         


Ser Thr Lys Trp His Asn Ser Tyr Arg Glu Pro Phe Glu Gln His 
        195                 200                 205         


