               SEQUENCE LISTING

<110> CHAIN Biotechnology Ltd

<120> Compositions and methods

<130> CHABM/P79622PC

<150> GB2019767.9
<151> 2020-12-15

<160> 33

<170> BiSSAP 1.3.6

<210> 1
<211> 502
<212> DNA
<213> Artificial Sequence


<220> 
<223> Fig 3 - OVA-ROP-FLAG nucleotide sequence

<400> 1
atataaaaat aagaagccat atgttggtgc tgttgcctga tgaagtctca ggccttgagc      60

agcttgagag tataatcaac tttgaaaaac tgactgaatg gaccagttct aatgttatgg     120

aattaagaat gaaaactgaa tggaccagtt ctaatgttat ggaagagagg aagatcaaag     180

tgtacttacc tcgcatgaag atggaggaaa aatacaacct cacatctgtc ttaatggctt     240

taagaatgaa aaaatacaac ctcacatctg tcttaatggc tatgggcatt actgacgtgt     300

ttagctcttc agccaatctg tctggcatct cctcagcaga gagcctgaag atatctttaa     360

gaatgaaaat ctcctcagca gagagcctga agatatctca agctgtccat gcagcacatg     420

cagaaatcaa tgaagcaggc agattaagaa tgaaagatta caaggatgac gacgataagt     480

aagctagcat aaaataagaa gc                                              502


<210> 2
<211> 153
<212> PRT
<213> Artificial Sequence


<220> 
<223> Fig 3 - OVA-ROP-FLAG amino acid sequence

<400> 2
Met Leu Val Leu Leu Pro Asp Glu Val Ser Gly Leu Glu Gln Leu Glu 
1               5                   10                  15      
Ser Ile Ile Asn Phe Glu Lys Leu Thr Glu Trp Thr Ser Ser Asn Val 
            20                  25                  30          
Met Glu Leu Arg Met Lys Thr Glu Trp Thr Ser Ser Asn Val Met Glu 
        35                  40                  45              
Glu Arg Lys Ile Lys Val Tyr Leu Pro Arg Met Lys Met Glu Glu Lys 
    50                  55                  60                  
Tyr Asn Leu Thr Ser Val Leu Met Ala Leu Arg Met Lys Lys Tyr Asn 
65                  70                  75                  80  
Leu Thr Ser Val Leu Met Ala Met Gly Ile Thr Asp Val Phe Ser Ser 
                85                  90                  95      
Ser Ala Asn Leu Ser Gly Ile Ser Ser Ala Glu Ser Leu Lys Ile Ser 
            100                 105                 110         
Leu Arg Met Lys Ile Ser Ser Ala Glu Ser Leu Lys Ile Ser Gln Ala 
        115                 120                 125             
Val His Ala Ala His Ala Glu Ile Asn Glu Ala Gly Arg Leu Arg Met 
    130                 135                 140                 
Lys Asp Tyr Lys Asp Asp Asp Asp Lys 
145                 150             

<210> 3
<211> 497
<212> DNA
<213> Artificial Sequence


<220> 
<223> Fig 4 - HPV-ROP-FLAG nucleotide sequence

<400> 3
ataaaaataa gaagccatat gcatggagat acacctacat tgcatgaata tatgttagat      60

ttgcaaccag agacaactga tctctactgt tatgagcaat taaatgacag ctcagaggag     120

gagttaagaa tgaaagagca attaaatgac agctcagagg aggaggatga aatagatggt     180

ccagctggac aagcagaacc ggacagagcc cattacaata ttgtaacctt ttgttgcaag     240

ttaagaatga aacattacaa tattgtaacc ttttgttgca agtgtgactc tacgcttcgg     300

ttgtgcgtac aaagcacaca cgtagacatt cgtactttgg aagacctgtt aatgggctta     360

agaatgaaaa ttcgtacttt ggaagacctg ttaatgggca cactaggaat tgtgtgcccc     420

atctgttctc aaaaaccatt aagaatgaaa gattacaagg atgacgacga taagtaagct     480

agcataaaat aagaagc                                                    497


<210> 4
<211> 152
<212> PRT
<213> Artificial Sequence


<220> 
<223> Fig 4 - HPV-ROP-FLAG amino acid sequence

<400> 4
Met His Gly Asp Thr Pro Thr Leu His Glu Tyr Met Leu Asp Leu Gln 
1               5                   10                  15      
Pro Glu Thr Thr Asp Leu Tyr Cys Tyr Glu Gln Leu Asn Asp Ser Ser 
            20                  25                  30          
Glu Glu Glu Leu Arg Met Lys Glu Gln Leu Asn Asp Ser Ser Glu Glu 
        35                  40                  45              
Glu Asp Glu Ile Asp Gly Pro Ala Gly Gln Ala Glu Pro Asp Arg Ala 
    50                  55                  60                  
His Tyr Asn Ile Val Thr Phe Cys Cys Lys Leu Arg Met Lys His Tyr 
65                  70                  75                  80  
Asn Ile Val Thr Phe Cys Cys Lys Cys Asp Ser Thr Leu Arg Leu Cys 
                85                  90                  95      
Val Gln Ser Thr His Val Asp Ile Arg Thr Leu Glu Asp Leu Leu Met 
            100                 105                 110         
Gly Leu Arg Met Lys Ile Arg Thr Leu Glu Asp Leu Leu Met Gly Thr 
        115                 120                 125             
Leu Gly Ile Val Cys Pro Ile Cys Ser Gln Lys Pro Leu Arg Met Lys 
    130                 135                 140                 
Asp Tyr Lys Asp Asp Asp Asp Lys 
145                 150         

<210> 5
<211> 100
<212> PRT
<213> Artificial Sequence


<220> 
<223> wildtype ovalbumin amino acid (aa) sequence ranging from
      aa241-aa340

<400> 5
Ser Met Leu Val Leu Leu Pro Asp Glu Val Ser Gly Leu Glu Gln Leu 
1               5                   10                  15      
Glu Ser Ile Ile Asn Phe Glu Lys Leu Thr Glu Trp Thr Ser Ser Asn 
            20                  25                  30          
Val Met Glu Glu Arg Lys Ile Lys Val Tyr Leu Pro Arg Met Lys Met 
        35                  40                  45              
Glu Glu Lys Tyr Asn Leu Thr Ser Val Leu Met Ala Met Gly Ile Thr 
    50                  55                  60                  
Asp Val Phe Ser Ser Ser Ala Asn Leu Ser Gly Ile Ser Ser Ala Glu 
65                  70                  75                  80  
Ser Leu Lys Ile Ser Gln Ala Val His Ala Ala His Ala Glu Ile Asn 
                85                  90                  95      
Glu Ala Gly Arg 
            100 

<210> 6
<211> 142
<212> PRT
<213> Artificial Sequence


<220> 
<223> 142aa recombinant overlapping peptide denoted ROP-OVA

<400> 6
Ser Met Leu Val Leu Leu Pro Asp Glu Val Ser Gly Leu Glu Gln Leu 
1               5                   10                  15      
Glu Ser Ile Ile Asn Phe Glu Lys Leu Thr Glu Trp Thr Ser Ser Asn 
            20                  25                  30          
Val Met Glu Leu Arg Met Lys Thr Glu Trp Thr Ser Ser Asn Val Met 
        35                  40                  45              
Glu Glu Arg Lys Ile Lys Val Tyr Leu Pro Arg Met Lys Met Glu Glu 
    50                  55                  60                  
Lys Tyr Asn Leu Thr Ser Val Leu Met Ala Leu Arg Met Lys Lys Tyr 
65                  70                  75                  80  
Asn Leu Thr Ser Val Leu Met Ala Met Gly Ile Thr Asp Val Phe Ser 
                85                  90                  95      
Ser Ser Ala Asn Leu Ser Gly Ile Ser Ser Ala Glu Ser Leu Lys Ile 
            100                 105                 110         
Ser Leu Arg Met Lys Ile Ser Ser Ala Glu Ser Leu Lys Ile Ser Gln 
        115                 120                 125             
Ala Val His Ala Ala His Ala Glu Ile Asn Glu Ala Gly Arg 
    130                 135                 140         

<210> 7
<211> 98
<212> PRT
<213> Artificial Sequence


<220> 
<223> wildtype E7 protein aa sequence ranging from aa1-aa98

<400> 7
Met His Gly Asp Thr Pro Thr Leu His Glu Tyr Met Leu Asp Leu Gln 
1               5                   10                  15      
Pro Glu Thr Thr Asp Leu Tyr Cys Tyr Glu Gln Leu Asn Asp Ser Ser 
            20                  25                  30          
Glu Glu Glu Asp Glu Ile Asp Gly Pro Ala Gly Gln Ala Glu Pro Asp 
        35                  40                  45              
Arg Ala His Tyr Asn Ile Val Thr Phe Cys Cys Lys Cys Asp Ser Thr 
    50                  55                  60                  
Leu Arg Leu Cys Val Gln Ser Thr His Val Asp Ile Arg Thr Leu Glu 
65                  70                  75                  80  
Asp Leu Leu Met Gly Thr Leu Gly Ile Val Cys Pro Ile Cys Ser Gln 
                85                  90                  95      
Lys Pro 
        

<210> 8
<211> 140
<212> PRT
<213> Artificial Sequence


<220> 
<223> 140aa recombinant overlapping peptide denoted ROP-HPV

<400> 8
Met His Gly Asp Thr Pro Thr Leu His Glu Tyr Met Leu Asp Leu Gln 
1               5                   10                  15      
Pro Glu Thr Thr Asp Leu Tyr Cys Tyr Glu Gln Leu Asn Asp Ser Ser 
            20                  25                  30          
Glu Glu Glu Leu Arg Met Lys Glu Gln Leu Asn Asp Ser Ser Glu Glu 
        35                  40                  45              
Glu Asp Glu Ile Asp Gly Pro Ala Gly Gln Ala Glu Pro Asp Arg Ala 
    50                  55                  60                  
His Tyr Asn Ile Val Thr Phe Cys Cys Lys Leu Arg Met Lys His Tyr 
65                  70                  75                  80  
Asn Ile Val Thr Phe Cys Cys Lys Cys Asp Ser Thr Leu Arg Leu Cys 
                85                  90                  95      
Val Gln Ser Thr His Val Asp Ile Arg Thr Leu Glu Asp Leu Leu Met 
            100                 105                 110         
Gly Leu Arg Met Lys Ile Arg Thr Leu Glu Asp Leu Leu Met Gly Thr 
        115                 120                 125             
Leu Gly Ile Val Cys Pro Ile Cys Ser Gln Lys Pro 
    130                 135                 140 

<210> 9
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward primer of Pfdx

<400> 9
gtgtagtagc ctgtgaaata ag                                             22


<210> 10
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse primer; Genomic DNA downstream of pyrE

<400> 10
cccatgttgg atctcctgag                                                20


<210> 11
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward primer; Genomic DNA upstream of pyrE

<400> 11
gcaagtgcgg tgcagattgg                                                20


<210> 12
<211> 39
<212> DNA
<213> Artificial Sequence


<220> 
<223> FLAG tag

<400> 12
ttacttatcg tcgtcatcct tgtaatcttt cattcttaa                            39


<210> 13
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse primer; pyrE repair long homology arm

<400> 13
aaatattaac aagtaatgat tatccaaaac                                     30


<210> 14
<211> 47
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse primer; Pfdx - overhang into HPV-ROP

<400> 14
gcaatgtagg tgtatctcca tgcatatgta acacacctcc ttaaaaa                   47


<210> 15
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward primer; HPV-ROP - overhang of Pfdx

<400> 15
tttaaggagg tgtgttacat atgcatggag atacacctac                           40


<210> 16
<211> 44
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse primer; Pfdx - overhang of OVA1

<400> 16
catcaggcaa cagcaccaac atatgtaaca cacctcctta aaaa                      44


<210> 17
<211> 41
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward primer; OVA1 - overhang of Pfdx

<400> 17
tttaaggagg tgtgttcata tgttggtgct gttgcctgat g                         41


<210> 18
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> FLAG tag

<400> 18
Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               

<210> 19
<211> 21
<212> PRT
<213> Vibrio cholerae


<220> 
<223> CtxB signal sequence

<400> 19
Met Ile Lys Leu Lys Phe Gly Val Phe Phe Thr Val Leu Leu Ser Ser 
1               5                   10                  15      
Ala Tyr Ala His Gly 
            20      

<210> 20
<211> 614
<212> DNA
<213> Artificial Sequence


<220> 
<223> p0957_CtxB genomic sequence

<400> 20
gcggccgcgg gatactgcag aagatataat agattcaatg aaaatgtgga gtaaataaaa      60

aaatcggata gaaatatccg attttttatt taaaaagact taaaaaaagt gcttgactct     120

tggaatttta agaaaaaata tggtataatc atattacgta ataaaaatac atttatatag     180

taataccata taaatgccaa taattgataa aaaaattaaa ttcaattaaa tcacatgagc     240

gagtaagaat tcaaggaggt gtgttacata tgacaccaca aaacataaca gatttatgtg     300

ctgagtatca taatacacaa atatatacac ttaatgataa aatattttca tatacagaat     360

cattagctgg aaaaagagaa atggcaataa taacatttaa aaatggagca atatttcaag     420

ttgaagttcc tggaagtcaa cacattgatt cacaaaaaaa agcaatagaa agaatgaaag     480

atacattaag aatagcatac ttaacagaag caaaagttga aaaattatgt gtttggaata     540

ataaaacacc acatgcaata gcagcaatat caatggcaaa tgattataaa gatgacgatg     600

ataaataagc tagc                                                       614


<210> 21
<211> 112
<212> PRT
<213> Artificial Sequence


<220> 
<223> p0957_CtxB translated protein sequence

<400> 21
Met Thr Pro Gln Asn Ile Thr Asp Leu Cys Ala Glu Tyr His Asn Thr 
1               5                   10                  15      
Gln Ile Tyr Thr Leu Asn Asp Lys Ile Phe Ser Tyr Thr Glu Ser Leu 
            20                  25                  30          
Ala Gly Lys Arg Glu Met Ala Ile Ile Thr Phe Lys Asn Gly Ala Ile 
        35                  40                  45              
Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp Ser Gln Lys Lys 
    50                  55                  60                  
Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Ala Tyr Leu Thr Glu 
65                  70                  75                  80  
Ala Lys Val Glu Lys Leu Cys Val Trp Asn Asn Lys Thr Pro His Ala 
                85                  90                  95      
Ile Ala Ala Ile Ser Met Ala Asn Asp Tyr Lys Asp Asp Asp Asp Lys 
            100                 105                 110         



<210> 22
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> CH22 F Forward plasmid backbone primer, 5’ of p0957

<400> 22
gtacatcacc gacgagcaag                                                20


<210> 23
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> CH54 R Reverse plasmid backbone primer, 3’ of FLAG-TAA

<400> 23
gacttatcca gggtgctatc ttcg                                           24


<210> 24
<211> 124
<212> PRT
<213> Artificial Sequence


<220> 
<223> PD01556 - Fig. 9 Part 2 of 2

<400> 24
Met Ile Lys Leu Lys Phe Gly Val Phe Phe Thr Val Leu Leu Ser Ser 
1               5                   10                  15      
Ala Tyr Ala His Gly Thr Pro Gln Asn Ile Thr Asp Leu Cys Ala Glu 
            20                  25                  30          
Tyr His Asn Thr Gln Ile Tyr Thr Leu Asn Asp Lys Ile Phe Ser Tyr 
        35                  40                  45              
Thr Glu Ser Leu Ala Gly Lys Arg Glu Met Ala Ile Ile Thr Phe Lys 
    50                  55                  60                  
Asn Gly Ala Ile Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp 
65                  70                  75                  80  
Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Ala 
                85                  90                  95      
Tyr Leu Thr Glu Ala Lys Val Glu Lys Leu Cys Val Trp Asn Asn Lys 
            100                 105                 110         
Thr Pro His Ala Ile Ala Ala Ile Ser Met Ala Asn 
        115                 120                 

<210> 25
<211> 104
<212> PRT
<213> Artificial Sequence


<220> 
<223> Consensus - Fig. 9 Part 2 of 2

<400> 25
Gly Thr Pro Gln Asn Ile Thr Asp Leu Cys Ala Glu Tyr His Asn Thr 
1               5                   10                  15      
Gln Ile Tyr Thr Leu Asn Asp Lys Ile Phe Ser Tyr Thr Glu Ser Leu 
            20                  25                  30          
Ala Gly Lys Arg Glu Met Ala Ile Ile Thr Phe Lys Asn Gly Ala Ile 
        35                  40                  45              
Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp Ser Gln Lys Lys 
    50                  55                  60                  
Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Ala Tyr Leu Thr Glu 
65                  70                  75                  80  
Ala Lys Val Glu Lys Leu Cys Val Trp Asn Asn Lys Thr Pro His Ala 
                85                  90                  95      
Ile Ala Ala Ile Ser Met Ala Asn 
            100                 

<210> 26
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cathepsin S cleavage site - pg 12

<400> 26
Leu Arg Met Lys 
1               

<210> 27
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cathepsin B cleavage site - pg 12

<400> 27
Met Lys Arg Leu 
1               

<210> 28
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cathepsin K cleavage site - pg 12

<400> 28
His Pro Gly Gly 
1               

<210> 29
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<221> SITE
<222> 1
<223> Wherein Xaa is any natural amino acid

<220> 
<223> Cathepsin S cleavage site - pg 12

<400> 29
Xaa Asn Leu Arg 
1               

<210> 30
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<221> SITE
<222> 1
<223> Wherein Xaa is any natural amino acid

<220> 
<223> Cathepsin S cleavage site - pg 12

<400> 30
Xaa Pro Leu Arg 
1               

<210> 31
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<221> SITE
<222> 1
<223> Wherein Xaa is any natural amino acid

<220> 
<223> Cathepsin S cleavage site - pg 12

<400> 31
Xaa Ile Val Gln 
1               

<210> 32
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<221> SITE
<222> 1
<223> Wherein Xaa is any natural amino acid

<220> 
<223> Cathepsin S cleavage site - pg 12

<400> 32
Xaa Arg Met Lys 
1               

<210> 33
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cathepsin cleavage site - pg 13

<400> 33
Leu Arg Met Lys 
1               

