                         SEQUENCE LISTING

<110>  Universiteit Utrecht Holding B.V.
 
<120>  CATH2 derivatives for stimulating innate immune memory

<130>  P128835PC00

<140>  PCT/NL2021/050776
<141>  2021-12-17

<150>  EP 20215665.9
<151>  2020-12-18

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal peptide called CMAP27 or CATH2

<400>  1

Arg Phe Gly Arg Phe Leu Arg Lys Ile Arg Arg Phe Arg Pro Lys Val 
1               5                   10                  15      


Thr Ile Thr Ile Gln Gly Ser Ala Arg Phe Gly 
            20                  25          


<210>  2
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D-amino acid CATH2 derivative: D-C(1-26)


<220>
<221>  MISC_FEATURE
<222>  (1)..(26)
<223>  D-amino acid

<220>
<221>  MOD_RES
<222>  (26)..(26)
<223>  AMIDATION

<400>  2

Arg Phe Gly Arg Phe Leu Arg Lys Ile Arg Arg Phe Arg Pro Lys Val 
1               5                   10                  15      


Thr Ile Thr Ile Gln Gly Ser Ala Arg Phe 
            20                  25      


<210>  3
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D-amino acid CATH2 derivative: D-C(1-21)


<220>
<221>  MISC_FEATURE
<222>  (1)..(21)
<223>  D-amino acid

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  AMIDATION

<400>  3

Arg Phe Gly Arg Phe Leu Arg Lys Ile Arg Arg Phe Arg Pro Lys Val 
1               5                   10                  15      


Thr Ile Thr Ile Gln 
            20      


<210>  4
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D-amino acid CATH2 derivative: D-C(4-21)


<220>
<221>  MISC_FEATURE
<222>  (1)..(18)
<223>  D-amino acid

<220>
<221>  MOD_RES
<222>  (18)..(18)
<223>  AMIDATION

<400>  4

Arg Phe Leu Arg Lys Ile Arg Arg Phe Arg Pro Lys Val Thr Ile Thr 
1               5                   10                  15      


Ile Gln 
        


<210>  5
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D-amino acid CATH2 derivative: D-C(7-21)


<220>
<221>  MISC_FEATURE
<222>  (1)..(15)
<223>  D-amino acid

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  AMIDATION

<400>  5

Arg Lys Ile Arg Arg Phe Arg Pro Lys Val Thr Ile Thr Ile Gln 
1               5                   10                  15  


<210>  6
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D-amino acid CATH2 derivative: D-C(7-21) F/W


<220>
<221>  MISC_FEATURE
<222>  (1)..(15)
<223>  D-amino acid

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  AMIDATION

<400>  6

Arg Lys Ile Arg Arg Trp Arg Pro Lys Val Thr Ile Thr Ile Gln 
1               5                   10                  15  


<210>  7
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D-amino acid CATH2 derivative: D-C(7-21) F/Y


<220>
<221>  MISC_FEATURE
<222>  (1)..(15)
<223>  D-amino acid

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  AMIDATION

<400>  7

Arg Lys Ile Arg Arg Tyr Arg Pro Lys Val Thr Ile Thr Ile Gln 
1               5                   10                  15  


<210>  8
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D-amino acid CATH2 derivative: D-C(10-21) F/W


<220>
<221>  MISC_FEATURE
<222>  (1)..(12)
<223>  D-amino acid

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  AMIDATION

<400>  8

Arg Arg Trp Arg Pro Lys Val Thr Ile Thr Ile Gln 
1               5                   10          


<210>  9
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D-amino acid CATH2 derivative: D-C (1-15)


<220>
<221>  MISC_FEATURE
<222>  (1)..(15)
<223>  D-amino acid

<400>  9

Arg Phe Gly Arg Phe Leu Arg Lys Ile Arg Arg Phe Arg Pro Lys 
1               5                   10                  15  


<210>  10
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cycCMAP(1-21)[Lys8]


<220>
<221>  MISC_FEATURE
<222>  (1)..(21)
<223>  cyclic derivative

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  is Fmoc-L-bishomopropargylglycine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  azido resin attached

<400>  10

Arg Phe Gly Arg Phe Leu Arg Xaa Ile Arg Arg Phe Arg Pro Lys Val 
1               5                   10                  15      


Thr Ile Thr Ile Gln 
            20      


<210>  11
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cycCMAP(1-21)[Arg7]


<220>
<221>  MISC_FEATURE
<222>  (1)..(21)
<223>  cyclic derivative

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  is Fmoc-L-bishomopropargylglycine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  azido resin attached

<400>  11

Arg Phe Gly Arg Phe Leu Xaa Lys Ile Arg Arg Phe Arg Pro Lys Val 
1               5                   10                  15      


Thr Ile Thr Ile Gln 
            20      


<210>  12
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cycCMAP(1-21)[Leu6]


<220>
<221>  MISC_FEATURE
<222>  (1)..(12)
<223>  cyclic derivative

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  is Fmoc-L-bishomopropargylglycine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  azido resin attached

<400>  12

Arg Phe Gly Arg Phe Xaa Arg Lys Ile Arg Arg Phe Arg Pro Lys Val 
1               5                   10                  15      


Thr Ile Thr Ile Gln 
            20      


<210>  13
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cycCMAP(1-21)[Leu6],Phe2/Trp


<220>
<221>  MISC_FEATURE
<222>  (1)..(21)
<223>  cyclic derivative

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  is Fmoc-L-bishomopropargylglycine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  azido resin attached

<400>  13

Arg Trp Gly Arg Phe Xaa Arg Lys Ile Arg Arg Phe Arg Pro Lys Val 
1               5                   10                  15      


Thr Ile Thr Ile Gln 
            20      


<210>  14
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cycCMAP(1-21)[Leu6],Phe2,5/Trp


<220>
<221>  MISC_FEATURE
<222>  (1)..(21)
<223>  cyclic derivative

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  is Fmoc-L-bishomopropargylglycine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  azido resin attached

<400>  14

Arg Trp Gly Arg Trp Xaa Arg Lys Ile Arg Arg Phe Arg Pro Lys Val 
1               5                   10                  15      


Thr Ile Thr Ile Gln 
            20      


<210>  15
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cycCMAP(1-21)[Leu6],Phe2,5,12/Trp


<220>
<221>  MISC_FEATURE
<222>  (1)..(21)
<223>  cyclic derivative

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  is Fmoc-L-bishomopropargylglycine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  azido resin attached

<400>  15

Arg Trp Gly Arg Trp Xaa Arg Lys Ile Arg Arg Trp Arg Pro Lys Val 
1               5                   10                  15      


Thr Ile Thr Ile Gln 
            20      


<210>  16
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cycCMAP(1-21)[Leu6],Phe5,12/Trp


<220>
<221>  MISC_FEATURE
<222>  (1)..(21)
<223>  cyclic derivative

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  is Fmoc-L-bishomopropargylglycine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  azido resin attached

<400>  16

Arg Phe Gly Arg Trp Xaa Arg Lys Ile Arg Arg Trp Arg Pro Lys Val 
1               5                   10                  15      


Thr Ile Thr Ile Gln 
            20      


<210>  17
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cycCMAP(1-21)[Leu6],Phe12/Trp


<220>
<221>  MISC_FEATURE
<222>  (1)..(21)
<223>  cyclic derivative

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  is Fmoc-L-bishomopropargylglycine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  azido resin attached

<400>  17

Arg Phe Gly Arg Phe Xaa Arg Lys Ile Arg Arg Trp Arg Pro Lys Val 
1               5                   10                  15      


Thr Ile Thr Ile Gln 
            20      


<210>  18
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  I and RI equivalent of CATH2

<400>  18

Gly Phe Arg Ala Ser Gly Gln Ile Thr Ile Thr Val Lys Pro Arg Phe 
1               5                   10                  15      


Arg Arg Ile Lys Arg Leu Phe Arg Gly Phe Arg 
            20                  25          


<210>  19
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RI-CMAP27-derivative containing one or more D amino acids: 
       RI-C(1-21)

<400>  19

Gln Ile Thr Ile Thr Val Lys Pro Arg Phe Arg Arg Ile Lys Arg Leu 
1               5                   10                  15      


Phe Arg Gly Phe Arg 
            20      


<210>  20
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RI-CMAP27-derivative containing one or more D amino acids: 
       RI-C(4-21)

<400>  20

Gln Ile Thr Ile Thr Val Lys Pro Arg Phe Arg Arg Ile Lys Arg Leu 
1               5                   10                  15      


Phe Arg 
        


<210>  21
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RI-CMAP27-derivative containing one or more D amino acids: 
       RI-C(7-21)

<400>  21

Gln Ile Thr Ile Thr Val Lys Pro Arg Phe Arg Arg Ile Lys Arg 
1               5                   10                  15  


<210>  22
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RI-CMAP27-derivative containing one or more D amino acids: 
       RI-C(7-21)F/W

<400>  22

Gln Ile Thr Ile Thr Val Lys Pro Arg Trp Arg Arg Ile Lys Arg 
1               5                   10                  15  


<210>  23
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RI-CMAP27-derivative containing one or more D amino acids: 
       RI-C(7-21)F/Y

<400>  23

Gln Ile Thr Ile Thr Val Lys Pro Arg Tyr Arg Arg Ile Lys Arg 
1               5                   10                  15  


<210>  24
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RI-CMAP27-derivative containing one or more D amino acids: 
       RI-C(10-21)F/W

<400>  24

Gln Ile Thr Ile Thr Val Lys Pro Arg Trp Arg Arg 
1               5                   10          


<210>  25
<211>  26
<212>  PRT
<213>  Gallus gallus


<220>
<221>  MOD_RES
<222>  (26)..(26)
<223>  AMIDATION

<400>  25

Arg Phe Gly Arg Phe Leu Arg Lys Ile Arg Arg Phe Arg Pro Lys Val 
1               5                   10                  15      


Thr Ile Thr Ile Gln Gly Ser Ala Arg Phe 
            20                  25      


<210>  26
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D-amino acid analog, DCATH-2


<220>
<221>  MISC_FEATURE
<222>  (1)..(26)
<223>  D-amino acid

<220>
<221>  MOD_RES
<222>  (26)..(26)
<223>  AMIDATION

<400>  26

Arg Phe Gly Arg Phe Leu Arg Lys Ile Arg Arg Phe Arg Pro Lys Val 
1               5                   10                  15      


Thr Ile Thr Ile Gln Gly Ser Ala Arg Phe 
            20                  25      


<210>  27
<211>  37
<212>  PRT
<213>  Homo sapiens

<400>  27

Leu Leu Gly Asp Phe Phe Arg Lys Ser Lys Glu Lys Ile Gly Lys Glu 
1               5                   10                  15      


Phe Lys Arg Ile Val Gln Arg Ile Lys Asp Phe Leu Arg Asn Leu Val 
            20                  25                  30          


Pro Arg Thr Glu Ser 
        35          


<210>  28
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tamra-labeled DCATH-2


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Tamra-labeled

<220>
<221>  MISC_FEATURE
<222>  (1)..(26)
<223>  D-amino acid

<220>
<221>  MOD_RES
<222>  (26)..(26)
<223>  AMIDATION

<400>  28

Arg Phe Gly Arg Phe Leu Arg Lys Ile Arg Arg Phe Arg Pro Lys Val 
1               5                   10                  15      


Thr Ile Thr Ile Gln Gly Ser Ala Arg Phe 
            20                  25      


