                               SEQUENCE LISTING

<110> MERCK SHARP & DOHME CORP.
 
<120> S-METHYLTHIORIBOSE KINASE POLYPEPTIDES AND PROCESSES FOR MAKING
      AND USING S-METHYLTHIORIBOSE KINASE POLYPEPTIDES

<130> 25182-WO-PCT

<140>
<141>

<150> 63/278,284
<151> 2021-11-11

<150> 63/125,154
<151> 2020-12-14

<160> 13    

<170> PatentIn version 3.5

<210> 1
<211> 399
<212> PRT
<213> Klebsiella pneumoniae

<400> 1
Met Ser Gln Tyr His Thr Phe Thr Ala His Asp Ala Val Ala Tyr Ala 
1               5                   10                  15      


Gln Gln Phe Ala Gly Ile Asp Asn Pro Ser Glu Leu Val Ser Ala Gln 
            20                  25                  30          


Glu Val Gly Asp Gly Asn Leu Asn Leu Val Phe Lys Val Phe Asp Arg 
        35                  40                  45              


Gln Gly Val Ser Arg Ala Ile Val Lys Gln Ala Leu Pro Tyr Val Arg 
    50                  55                  60                  


Cys Val Gly Glu Ser Trp Pro Leu Thr Leu Asp Arg Ala Arg Leu Glu 
65                  70                  75                  80  


Ala Gln Thr Leu Val Ala His Tyr Gln His Ser Pro Gln His Thr Val 
                85                  90                  95      


Lys Ile His His Phe Asp Pro Glu Leu Ala Val Met Val Met Glu Asp 
            100                 105                 110         


Leu Ser Asp His Arg Ile Trp Arg Gly Glu Leu Ile Ala Asn Val Tyr 
        115                 120                 125             


Tyr Pro Gln Ala Ala Arg Gln Leu Gly Asp Tyr Leu Ala Gln Val Leu 
    130                 135                 140                 


Phe His Thr Ser Asp Phe Tyr Leu His Pro His Glu Lys Lys Ala Gln 
145                 150                 155                 160 


Val Ala Gln Phe Ile Asn Pro Ala Met Cys Glu Ile Thr Glu Asp Leu 
                165                 170                 175     


Phe Phe Asn Asp Pro Tyr Gln Ile His Glu Arg Asn Asn Tyr Pro Ala 
            180                 185                 190         


Glu Leu Glu Ala Asp Val Ala Ala Leu Arg Asp Asp Ala Gln Leu Lys 
        195                 200                 205             


Leu Ala Val Ala Ala Leu Lys His Arg Phe Phe Ala His Ala Glu Ala 
    210                 215                 220                 


Leu Leu His Gly Asp Ile His Ser Gly Ser Ile Phe Val Ala Glu Gly 
225                 230                 235                 240 


Ser Leu Lys Ala Ile Asp Ala Glu Phe Gly Tyr Phe Gly Pro Ile Gly 
                245                 250                 255     


Phe Asp Ile Gly Thr Ala Ile Gly Asn Leu Leu Leu Asn Tyr Cys Gly 
            260                 265                 270         


Leu Pro Gly Gln Leu Gly Ile Arg Asp Ala Ala Ala Ala Arg Glu Gln 
        275                 280                 285             


Arg Leu Asn Asp Ile His Gln Leu Trp Thr Thr Phe Ala Glu Arg Phe 
    290                 295                 300                 


Gln Ala Leu Ala Ala Glu Lys Thr Arg Asp Ala Ala Leu Ala Tyr Pro 
305                 310                 315                 320 


Gly Tyr Ala Ser Ala Phe Leu Lys Lys Val Trp Ala Asp Ala Val Gly 
                325                 330                 335     


Phe Cys Gly Ser Glu Leu Ile Arg Arg Ser Val Gly Leu Ser His Val 
            340                 345                 350         


Ala Asp Ile Asp Thr Ile Gln Asp Asp Ala Met Arg His Glu Cys Leu 
        355                 360                 365             


Arg His Ala Ile Thr Leu Gly Arg Ala Leu Ile Val Leu Ala Glu Arg 
    370                 375                 380                 


Ile Asp Ser Val Asp Glu Leu Leu Ala Arg Val Arg Gln Tyr Ser 
385                 390                 395                 


<210> 2
<211> 1197
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> codon optimized sequence encoding wild-type polypeptide

<400> 2
atgagccagt atcatacctt caccgcgcat gatgcggtgg cgtatgcgca gcaatttgcg       60

ggcattgata acccgagcga gctggttagc gcgcaagaag ttggtgacgg caacctgaac      120

ctggtgttca aggtttttga tcgtcagggt gtgagccgtg cgatcgttaa acaagcgctg      180

ccgtacgtgc gttgcgttgg tgaaagctgg ccgctgaccc tggaccgtgc gcgtctggaa      240

gcgcagaccc tggtggcgca ctatcagcac agcccgcaac acaccgttaa gatccaccac      300

ttcgatccgg agctggcggt gatggttatg gaagacctga gcgatcaccg tatttggcgt      360

ggtgagctga tcgcgaacgt gtactatccg caggcggcgc gtcaactggg tgactacctg      420

gcgcaggttc tgttccacac cagcgatttt tatctgcacc cgcacgagaa gaaagcgcag      480

gtggcgcaat tcattaaccc ggcgatgtgc gagatcaccg aagacctgtt ctttaacgat      540

ccgtaccaga ttcacgaacg taacaactat ccggcggagc tggaagcgga tgtggcggcg      600

ctgcgtgatg atgcgcaact gaagctggcg gttgcggcgc tgaaacaccg tttctttgcg      660

catgcggagg cgctgctgca tggtgacatt cacagcggca gcatcttcgt tgcggagggt      720

agcctgaagg cgatcgacgc ggaattcggt tactttggcc cgatcggttt tgatattggt      780

accgcgatcg gcaacctgct gctgaactat tgcggtctgc cgggtcaact gggtattcgt      840

gatgcggcgg cggcgcgtga acagcgtctg aacgatatcc accaactgtg gaccaccttc      900

gcggagcgtt ttcaagcgct ggcggcggaa aagacccgtg acgcggcgct ggcgtacccg      960

ggttatgcga gcgcgttcct gaagaaagtg tgggcggatg cggttggttt ttgcggcagc     1020

gagctgattc gtcgtagcgt gggcctgagc cacgttgcgg acatcgatac cattcaggac     1080

gatgcgatgc gtcacgaatg cctgcgtcac gcgatcaccc tgggtcgtgc gctgattgtt     1140

ctggcggagc gtatcgacag cgtggatgaa ctgctggcgc gtgttcgtca atacagc        1197


<210> 3
<211> 407
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> MTR kinase AA seq

<400> 3
Met Ser Gln Tyr His Thr Phe Thr Ala His Asp Ala Val Ala Tyr Ala 
1               5                   10                  15      


Gln Gln Phe Ala Gly Ile Asp Asn Pro Ser Glu Leu Val Ser Ala Gln 
            20                  25                  30          


Glu Val Gly Asp Gly Asn Leu Asn Leu Val Phe Lys Val Phe Asp Arg 
        35                  40                  45              


Gln Gly Val Ser Arg Ala Ile Val Lys Gln Ala Leu Pro Tyr Val Arg 
    50                  55                  60                  


Cys Val Gly Glu Ser Trp Pro Leu Thr Leu Asp Arg Ala Arg His Glu 
65                  70                  75                  80  


Ala Gln Thr Leu Val Ala His Tyr Gln His Ser Pro Gln His Thr Val 
                85                  90                  95      


Lys Ile His His Phe Asp Pro Glu Leu Ala Val Met Val Met Glu Asp 
            100                 105                 110         


Leu Ser Asp His Arg Ile Trp Arg Gly Glu Leu Ile Ala Asn Val Tyr 
        115                 120                 125             


Tyr Pro Gln Ala Ala Arg Gln Leu Gly Asp Tyr Leu Ala Gln Val Leu 
    130                 135                 140                 


Phe His Thr Ser Asp Phe Tyr Leu His Pro His Glu Lys Lys Ala Gln 
145                 150                 155                 160 


Val Ala Gln Phe Ile Asn Pro Ala Met Cys Glu Ile Thr Glu Asp Leu 
                165                 170                 175     


Phe Phe Asn Asp Pro Tyr Gln Ile His Glu Arg Asn Asn Tyr Pro Ala 
            180                 185                 190         


Glu Leu Glu Ala Asp Val Ala Ala Leu Arg Asp Asp Ala Gln Leu Lys 
        195                 200                 205             


Leu Ala Val Ala Ala Leu Lys His Arg Phe Phe Ala His Ala Glu Ala 
    210                 215                 220                 


Leu Leu His Gly Asp Ile His Ser Gly Ser Ile Phe Val Ala Glu Gly 
225                 230                 235                 240 


Ser Leu Lys Ala Ile Asp Ala Glu Phe Gly Tyr Phe Gly Pro Ile Gly 
                245                 250                 255     


Phe Asp Ile Gly Thr Ala Ile Gly Asn Leu Leu Leu Asn Tyr Cys Gly 
            260                 265                 270         


Leu Pro Gly Gln Leu Gly Ile Arg Asp Ala Ala Ala Ala Arg Glu Gln 
        275                 280                 285             


Arg Leu Asn Asp Ile His Gln Leu Trp Thr Thr Phe Ala Glu Arg Phe 
    290                 295                 300                 


Gln Ala Leu Ala Ala Glu Lys Thr Arg Asp Ala Ala Leu Ala Tyr Pro 
305                 310                 315                 320 


Gly Tyr Ala Ser Ala Phe Leu Lys Lys Val Trp Ala Asp Ala Val Gly 
                325                 330                 335     


Phe Cys Gly Ser Glu Leu Ile Arg Arg Ser Val Gly Leu Ser His Val 
            340                 345                 350         


Ala Asp Ile Asp Thr Ile Gln Asp Asp Ala Met Arg His Glu Cys Leu 
        355                 360                 365             


Arg His Ala Ile Thr Leu Gly Arg Ala Leu Ile Val Leu Ala Glu Arg 
    370                 375                 380                 


Ile Asp Ser Val Asp Glu Leu Leu Ala Arg Val Arg Gln Tyr Ser Leu 
385                 390                 395                 400 


Glu His His His His His His 
                405         


<210> 4
<211> 1224
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> MTR kinase DNA seq

<400> 4
atgagccagt atcatacctt caccgcgcat gatgcggtgg cgtatgcgca gcaatttgcg       60

ggcattgata acccgagcga gctggttagc gcgcaagaag ttggtgacgg caacctgaac      120

ctggtgttca aggtttttga tcgtcagggt gtgagccgtg cgatcgttaa acaagcgctg      180

ccgtacgtgc gttgcgttgg tgaaagctgg ccgctgaccc tggaccgtgc gcgtcatgaa      240

gcgcagaccc tggtggcgca ctatcagcac agcccgcaac acaccgttaa gatccaccac      300

ttcgatccgg agctggcggt gatggttatg gaagacctga gcgatcaccg tatttggcgt      360

ggtgagctga tcgcgaacgt gtactatccg caggcggcgc gtcaactggg tgactacctg      420

gcgcaggttc tgttccacac cagcgatttt tatctgcacc cgcacgagaa gaaagcgcag      480

gtggcgcaat tcattaaccc ggcgatgtgc gagatcaccg aagacctgtt ctttaacgat      540

ccgtaccaga ttcacgaacg taacaactat ccggcggagc tggaagcgga tgtggcggcg      600

ctgcgtgatg atgcgcaact gaagctggcg gttgcggcgc tgaaacaccg tttctttgcg      660

catgcggagg cgctgctgca tggtgacatt cacagcggca gcatcttcgt tgcggagggt      720

agcctgaagg cgatcgacgc ggaattcggt tactttggcc cgatcggttt tgatattggt      780

accgcgatcg gcaacctgct gctgaactat tgcggtctgc cgggtcaact gggtattcgt      840

gatgcggcgg cggcgcgtga acagcgtctg aacgatatcc accaactgtg gaccaccttc      900

gcggagcgtt ttcaagcgct ggcggcggaa aagacccgtg acgcggcgct ggcgtacccg      960

ggttatgcga gcgcgttcct gaagaaagtg tgggcggatg cggttggttt ttgcggcagc     1020

gagctgattc gtcgtagcgt gggcctgagc cacgttgcgg acatcgatac cattcaggac     1080

gatgcgatgc gtcacgaatg cctgcgtcac gcgatcaccc tgggtcgtgc gctgattgtt     1140

ctggcggagc gtatcgacag cgtggatgaa ctgctggcgc gtgttcgtca atacagcctc     1200

gagcaccacc accaccacca ctga                                            1224


<210> 5
<211> 407
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> MTR kinase AA seq

<400> 5
Met Ser Gln Tyr His Thr Phe Thr Ala His Asp Ala Val Ala Tyr Ala 
1               5                   10                  15      


Gln Gln Phe Ala Gly Ile Asp Asn Pro Ser Glu Leu Val Ser Ala Gln 
            20                  25                  30          


Glu Val Gly Asp Gly Asn Leu Asn Leu Val Phe Lys Val Phe Asp Arg 
        35                  40                  45              


Gln Gly Val Ser Arg Ala Ile Val Lys Gln Ala Leu Pro Tyr Pro Arg 
    50                  55                  60                  


Cys Val Gly Glu Ser Trp Pro Leu Thr Leu Asp Arg Ala Arg His Glu 
65                  70                  75                  80  


Ala Gln Thr Leu Val Ala His Tyr Gln His Ser Pro Gln His Thr Val 
                85                  90                  95      


Lys Ile His His Phe Asp Pro Glu Leu Ala Val Met Val Met Glu Asp 
            100                 105                 110         


Leu Ser Asp His Arg Ile Trp Arg Gly Glu Leu Ile Ala Asn Val Tyr 
        115                 120                 125             


Tyr Pro Gln Ala Ala Arg Gln Leu Gly Asp Tyr Leu Ala Gln Val Leu 
    130                 135                 140                 


Phe His Thr Ser Asp Phe Tyr Leu His Pro His Glu Lys Lys Ala Gln 
145                 150                 155                 160 


Val Ala Gln Phe Ile Asn Pro Ala Met Cys Glu Ile Ser Glu Asp Leu 
                165                 170                 175     


Ile Phe Asn Asp Pro Tyr Gln Ile His Glu Arg Asn Asn Tyr Pro Ala 
            180                 185                 190         


Glu Leu Glu Ala Asp Val Ala Ala Leu Arg Asp Asp Ala Gln Leu Lys 
        195                 200                 205             


Leu Ala Val Ala Ala Leu Lys His Arg Phe Phe Ala His Ala Glu Ala 
    210                 215                 220                 


Leu Leu His Gly Asp Leu His Ser Gly Ser Ile Phe Val Ala Glu Gly 
225                 230                 235                 240 


Ser Leu Lys Ala Ile Asp Ala Glu Phe Gly Tyr Phe Gly Pro Ile Gly 
                245                 250                 255     


Phe Asp Ile Gly Thr Ala Ile Gly Asn Leu Leu Leu Asn Tyr Cys Gly 
            260                 265                 270         


Leu Pro Gly Gln Leu Gly Ile Arg Asp Ala Ala Ala Ala Arg Glu Gln 
        275                 280                 285             


Arg Leu Asn Asp Ile His Gln Leu Trp Thr Thr Phe Ala Glu Arg Phe 
    290                 295                 300                 


Gln Ala Leu Ala Ala Glu Lys Thr Arg Asp Ala Ala Leu Ala Tyr Pro 
305                 310                 315                 320 


Gly Tyr Ala Ser Ala Phe Leu Lys Lys Val Trp Ala Asp Ala Val Gly 
                325                 330                 335     


Phe Cys Gly Ser Glu Leu Ile Arg Arg Ser Val Gly Leu Ser His Val 
            340                 345                 350         


Ala Asp Ile Asp Thr Ile Gln Asp Asp Ala Met Arg His Glu Cys Leu 
        355                 360                 365             


Arg His Ala Ile Thr Leu Gly Arg Ala Leu Ile Val Leu Ala Glu Arg 
    370                 375                 380                 


Ile Asp Ser Val Asp Glu Leu Leu Ala Arg Val Arg Gln Tyr Ser Leu 
385                 390                 395                 400 


Glu His His His His His His 
                405         


<210> 6
<211> 1224
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> MTR kinase DNA seq

<400> 6
atgagccagt atcatacctt caccgcgcat gatgcggtgg cgtatgcgca gcaatttgcg       60

ggcattgata acccgagcga gctggttagc gcgcaagaag ttggtgacgg caacctgaac      120

ctggtgttca aggtttttga tcgtcagggt gtgagccgtg cgatcgttaa acaagcgctg      180

ccgtaccccc gttgcgttgg tgaaagctgg ccgctgaccc tggaccgtgc gcgtcatgaa      240

gcgcagaccc tggtggcgca ctatcagcac agcccgcaac acaccgttaa gatccaccac      300

ttcgatccgg agctggcggt gatggttatg gaagacctga gcgatcaccg tatttggcgt      360

ggtgagctga tcgcgaacgt gtactatccg caggcggcgc gtcaactggg tgactacctg      420

gcgcaggttc tgttccacac cagcgatttt tatctgcacc cgcacgagaa gaaagcgcag      480

gtggcgcaat tcattaaccc ggcgatgtgc gagatcagcg aagacctgat ttttaacgat      540

ccgtaccaga ttcacgaacg taacaactat ccggcggagc tggaagcgga tgtggcggcg      600

ctgcgtgatg atgcgcaact gaagctggcg gttgcggcgc tgaaacaccg tttctttgcg      660

catgcggagg cgctgctgca tggtgacctt cacagcggca gcatcttcgt tgcggagggt      720

agcctgaagg cgatcgacgc ggaattcggt tactttggcc cgatcggttt tgatattggt      780

accgcgatcg gcaacctgct gctgaactat tgcggtctgc cgggtcaact gggtattcgt      840

gatgcggcgg cggcgcgtga acagcgtctg aacgatatcc accaactgtg gaccaccttc      900

gcggagcgtt ttcaagcgct ggcggcggaa aagacccgtg acgcggcgct ggcgtacccg      960

ggttatgcga gcgcgttcct gaagaaagtg tgggcggatg cggttggttt ttgcggcagc     1020

gagctgattc gtcgtagcgt gggcctgagc cacgttgcgg acatcgatac cattcaggac     1080

gatgcgatgc gtcacgaatg cctgcgtcac gcgatcaccc tgggtcgtgc gctgattgtt     1140

ctggcggagc gtatcgacag cgtggatgaa ctgctggcgc gtgttcgtca atacagcctc     1200

gagcaccacc accaccacca ctga                                            1224


<210> 7
<211> 407
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> MTR kinase AA seq

<400> 7
Met Ser Gln Tyr His Thr Phe Thr Ala Asp Asp Ala Val Ala Tyr Ala 
1               5                   10                  15      


Gln Gln Phe Ala Gly Ile Asp Asn Pro Ser Glu Leu Val Ser Ala Gln 
            20                  25                  30          


Glu Val Gly Asp Gly Asn Leu Asn Leu Val Phe Lys Val Phe Asp Arg 
        35                  40                  45              


Gln Gly Val Ser Arg Ala Ile Val Lys Gln Ala Leu Pro Tyr Pro Arg 
    50                  55                  60                  


Arg Val Gly Pro Ser Trp Pro Leu Thr Leu Asp Arg Ala Arg His Glu 
65                  70                  75                  80  


Ala Gln Thr Leu Val Ala His Tyr Gln His Ser Pro Gln His Thr Val 
                85                  90                  95      


Lys Ile His His Phe Asp Pro Glu Leu Ala Val Met Val Met Glu Asp 
            100                 105                 110         


Leu Ser Asp His Arg Ile Trp Arg Gly Glu Leu Ile Ala Asn Val Tyr 
        115                 120                 125             


Tyr Pro Gln Ala Ala Arg Gln Leu Gly Asp Tyr Leu Ala Gln Val Leu 
    130                 135                 140                 


Phe His Thr Ser Asp Phe Tyr Leu His Pro His Glu Lys Lys Ala Gln 
145                 150                 155                 160 


Val Ala Gln Phe Ile Asn Pro Ala Met Cys Glu Ile Ser Glu Asp Leu 
                165                 170                 175     


Val Phe Asn Asp Pro Tyr Gln Ile His Glu Arg Asn Asn Tyr Pro Ala 
            180                 185                 190         


Glu Leu Glu Ala Asp Val Ala Ala Leu Arg Asp Asp Ala Gln Leu Lys 
        195                 200                 205             


Leu Ala Val Ala Ala Leu Lys His Arg Phe Phe Ala His Ala Glu Ala 
    210                 215                 220                 


Leu Leu His Gly Asp Leu His Thr Gly Ser Ile Phe Val Ala Glu Gly 
225                 230                 235                 240 


Ser Leu Lys Val Ile Asp Ala Glu Phe Gly Tyr Phe Gly Pro Ile Gly 
                245                 250                 255     


Phe Asp Ile Gly Thr Ala Ile Gly Asn Leu Leu Leu Asn Tyr Cys Gly 
            260                 265                 270         


Leu Pro Gly Gln Leu Gly Ile Arg Asp Ala Ala Ala Ala Arg Glu Gln 
        275                 280                 285             


Arg Leu Asn Asp Ile His Gln Leu Trp Thr Thr Phe Ala Glu Arg Phe 
    290                 295                 300                 


Gln Ala Leu Ala Ala Glu Lys Thr Arg Asp Ala Ala Leu Ala Tyr Pro 
305                 310                 315                 320 


Gly Tyr Ala Ser Ala Phe Leu Lys Lys Val Trp Ala Asp Ala Val Gly 
                325                 330                 335     


Phe Cys Gly Ser Glu Leu Ile Arg Arg Ser Val Gly Leu Ala His Val 
            340                 345                 350         


Ala Asp Ile Asp Thr Ile Gln Asp Asp Ala Met Arg His Glu Cys Leu 
        355                 360                 365             


Arg Glu Ala Ile Thr Leu Gly Arg Ala Leu Ile Val Leu Ala Glu Arg 
    370                 375                 380                 


Ile Asp Ser Val Asp Glu Leu Leu Ala Arg Val Arg Gln Tyr Ser Leu 
385                 390                 395                 400 


Glu His His His His His His 
                405         


<210> 8
<211> 1224
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> MTR kinase DNA seq

<400> 8
atgagccagt atcatacctt caccgcggat gatgcggtgg cgtatgcgca gcaatttgcg       60

ggcattgata acccgagcga gctggttagc gcgcaagaag ttggtgacgg caacctgaac      120

ctggtgttca aggtttttga tcgtcagggt gtgagccgtg cgatcgttaa acaagcgctg      180

ccgtaccccc gtcgcgttgg tccgagctgg ccgctgaccc tggaccgtgc ccgtcatgaa      240

gcgcagaccc tggttgcgca ctatcagcac agcccgcaac acaccgttaa gatccaccac      300

ttcgatccgg agctggcggt gatggttatg gaagacctga gcgatcaccg tatttggcgt      360

ggtgagctga tcgcgaacgt gtactatccg caggcggcgc gtcaactggg tgactacctg      420

gcgcaggttc tgttccacac cagcgatttt tatctgcacc cgcacgagaa gaaagcgcag      480

gtggcgcaat tcattaaccc ggccatgtgc gaaatcagcg aagacctggt gtttaacgat      540

ccgtaccaga ttcacgaacg taacaactat ccggcggagc tggaagcgga tgtggcggcg      600

ctgcgtgatg atgcgcaact gaagctggcg gttgcggcgc tgaaacaccg tttctttgcg      660

catgcggagg cgctgctgca tggtgacctt cacaccggca gcatcttcgt tgcggagggt      720

agcctgaagg tgatcgacgc ggaattcggt tactttggcc cgatcggttt tgatattggt      780

accgcgatcg gcaacctgct gctgaactat tgcggtctgc cgggtcaact gggtattcgt      840

gatgcggcgg cggcgcgtga acagcgtctg aacgatatcc accaactgtg gaccaccttc      900

gcggagcgtt ttcaagcgct ggcggcggaa aagacccgtg acgcggcgct ggcgtacccg      960

ggttatgcga gcgcgttcct gaagaaagtg tgggcggatg cggttggttt ttgcggcagc     1020

gagctgattc gtcgtagcgt gggcctggcg cacgttgcgg acatcgatac cattcaggac     1080

gatgcgatgc gtcacgaatg cctgcgtgaa gcgatcaccc tgggtcgtgc gctgattgtt     1140

ctggcggagc gcatcgacag cgtggatgaa ctgctggcgc gtgttcgtca atacagcctc     1200

gagcaccacc accaccacca ctga                                            1224


<210> 9
<211> 407
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> MTR kinase AA seq

<400> 9
Met Ser Gln Tyr His Thr Phe Thr Ala Asp Asp Ala Val Ala Tyr Ala 
1               5                   10                  15      


Gln Gln Phe Ala Gly Ile Asp Asn Pro Ser Glu Leu Val Ser Ala Gln 
            20                  25                  30          


Glu Val Gly Asp Gly Asn Leu Asn Leu Val Phe Lys Val Phe Asp Arg 
        35                  40                  45              


Gln Gly Val Ser Arg Ala Ile Val Lys Gln Ala Leu Pro Tyr Pro Arg 
    50                  55                  60                  


Ala Val Gly Pro Ser Trp Pro Leu Thr Leu Asp Arg Ala Arg His Glu 
65                  70                  75                  80  


Ala Gln Thr Leu Val Ala His Tyr Gln His Ser Pro Gln His Thr Val 
                85                  90                  95      


Lys Ile His His Phe Asp Pro Glu Leu Ala Val Met Val Met Glu Asp 
            100                 105                 110         


Leu Ser Asp His Arg Ile Trp Arg Gly Glu Leu Ile Ala Asn Val Tyr 
        115                 120                 125             


Tyr Pro Gln Ala Ala Arg Gln Leu Gly Asp Tyr Leu Ala Gln Val Leu 
    130                 135                 140                 


Phe His Thr Ser Asp Phe Tyr Leu His Pro His Glu Lys Lys Ala Gln 
145                 150                 155                 160 


Val Ala Gln Phe Ile Asn Pro Gly Met Cys Glu Ile Ser Glu Asp Leu 
                165                 170                 175     


Ser Phe Asn Asp Pro Tyr Gln Ile His Glu Arg Asn Asn Tyr Pro Ala 
            180                 185                 190         


Glu Leu Glu Ala Asp Val Ala Ala Leu Arg Asp Asp Ala Gln Leu Lys 
        195                 200                 205             


Leu Ala Val Ala Ala Leu Lys His Arg Phe Phe Ala His Ala Glu Ala 
    210                 215                 220                 


Leu Leu His Gly Asp Leu His Ser Gly Ser Ile Phe Val Ala Glu Gly 
225                 230                 235                 240 


Ser Leu Lys Val Ile Asp Ala Glu Phe Gly Tyr Phe Gly Pro Ile Gly 
                245                 250                 255     


Phe Asp Ile Gly Thr Ala Ile Gly Asn Leu Leu Leu Asn Tyr Cys Gly 
            260                 265                 270         


Leu Pro Gly Gln Leu Gly Ile Arg Asp Ala Ala Ala Ala Arg Glu Gln 
        275                 280                 285             


Arg Leu Asn Asp Ile His Gln Leu Trp Thr Thr Phe Ala Glu Arg Phe 
    290                 295                 300                 


Gln Ala Leu Ala Ala Glu Lys Thr Arg Asp Ala Ala Leu Ala Tyr Pro 
305                 310                 315                 320 


Gly Tyr Ala Ser Ala Phe Leu Lys Lys Val Trp Ala Asp Ala Val Gly 
                325                 330                 335     


Phe Cys Gly Ser Glu Leu Ile Arg Arg Ser Val Gly Leu Ser His Val 
            340                 345                 350         


Ala Asp Ile Asp Thr Ile Gln Asp Asp Ala Met Arg His Glu Cys Leu 
        355                 360                 365             


Arg His Ala Ile Thr Leu Gly Arg Ala Leu Ile Val Leu Ala Glu Thr 
    370                 375                 380                 


Ile Asp Ser Val Asp Glu Leu Leu Ala Arg Val Arg Gln Tyr Ser Leu 
385                 390                 395                 400 


Glu His His His His His His 
                405         


<210> 10
<211> 1224
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> MTR kinase DNA seq

<400> 10
atgagccagt atcatacctt caccgcggat gatgcggtgg cgtatgcgca gcaatttgcg       60

ggcattgata acccgagcga gctggttagc gcgcaagaag ttggtgacgg caacctgaac      120

ctggtgttca aggtttttga tcgtcagggt gtgagccgtg cgatcgttaa acaagcgctg      180

ccgtaccccc gtgcggttgg tccgagctgg ccgctgaccc tggaccgtgc gcgtcatgaa      240

gcgcagaccc tggtggcgca ctatcagcac agcccgcaac acaccgttaa gatccaccac      300

ttcgatccgg agctggcggt gatggttatg gaagacctga gcgatcaccg tatttggcgt      360

ggtgagctga tcgcgaacgt gtactatccg caggcggcgc gtcaactggg tgactacctg      420

gcgcaggttc tgttccacac cagcgatttt tatctgcacc cgcacgagaa gaaagcgcag      480

gtggcgcaat tcattaaccc gggcatgtgc gaaatcagcg aagacctgag ctttaacgat      540

ccgtaccaga ttcacgaacg taacaactat ccggcggagc tggaagcgga tgtggcggcg      600

ctgcgtgatg atgcgcaact gaagctggcg gttgcggcgc tgaaacaccg tttctttgcg      660

catgcggagg cgctgctgca tggtgacctt cacagcggca gcatcttcgt tgcggagggt      720

agcctgaagg tgatcgacgc ggaattcggt tactttggcc cgatcggttt tgatattggt      780

accgcgatcg gcaacctgct gctgaactat tgcggtctgc cgggtcaact gggtattcgt      840

gatgcggcgg cggcgcgtga acagcgtctg aacgatatcc accaactgtg gaccaccttc      900

gcggagcgtt ttcaagcgct ggcggcggaa aagacccgtg acgcggcgct ggcgtacccg      960

ggttatgcga gcgcgttcct gaagaaagtg tgggcggatg cggttggttt ttgcggcagc     1020

gagctgattc gtcgtagcgt gggcctgtcg cacgttgcgg acatcgatac cattcaggac     1080

gatgcgatgc gtcacgaatg cctgcgtcac gcgatcaccc tgggtcgtgc gctgattgtt     1140

ctggcggaga ccatcgacag cgtggatgaa ctgctggcgc gtgttcgtca atacagcctc     1200

gagcaccacc accaccacca ctga                                            1224


<210> 11
<211> 407
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> MTR kinase AA seq

<400> 11
Met Ser Gln Tyr His Thr Phe Thr Ala Asp Asp Ala Val Ala Tyr Ala 
1               5                   10                  15      


Gln Gln Phe Ala Gly Ile Asp Asn Pro Ser Glu Leu Val Ser Ala Gln 
            20                  25                  30          


Glu Val Gly Asp Gly Asn Leu Asn Leu Val Phe Lys Val Phe Asp Arg 
        35                  40                  45              


Gln Gly Val Ser Arg Ala Ile Val Lys Gln Ala Leu Pro Tyr Pro Arg 
    50                  55                  60                  


Arg Val Gly Pro Ser Trp Pro Leu Thr Leu Asp Arg Ala Arg His Glu 
65                  70                  75                  80  


Ala Gln Thr Leu Val Ala His Tyr Gln His Ser Pro Gln His Thr Val 
                85                  90                  95      


Lys Ile Phe His Phe Asp Pro Glu Leu Ala Val Met Val Met Glu Asp 
            100                 105                 110         


Leu Ser Asp His Arg Ile Trp Arg Gly Glu Leu Ile Ala Asn Val Tyr 
        115                 120                 125             


Tyr Pro Gln Ala Ala Arg Gln Leu Gly Asp Tyr Leu Ala Gln Val Leu 
    130                 135                 140                 


Phe His Thr Ser Asp Phe Tyr Leu His Pro His Glu Lys Lys Arg Gln 
145                 150                 155                 160 


Val Ala Gln Phe Ile Asn Pro Ala Met Cys Gly Ile Ser Glu Asp Leu 
                165                 170                 175     


Val Phe Asn Asp Pro Tyr Gln Ile His Glu Arg Asn Asn Tyr Pro Ala 
            180                 185                 190         


Glu Leu Glu Ala Gln Val Ala Ala Leu Arg Asp Asp Ala Gln Leu Lys 
        195                 200                 205             


Leu Ala Val Ala Ala Leu Lys His Arg Phe Phe Ala His Ala Glu Ala 
    210                 215                 220                 


Leu Leu His Gly Asp Leu His Thr Gly Ser Ile Phe Val Lys Glu Gly 
225                 230                 235                 240 


Ser Leu Lys Val Ile Asp Ala Glu Phe Gly Tyr Phe Gly Pro Ile Gly 
                245                 250                 255     


Phe Asp Ile Gly Thr Ala Ile Gly Asn Leu Leu Leu Asn Tyr Cys Gly 
            260                 265                 270         


Leu Pro Gly Gln Leu Gly Ile Arg Asp Ala Ala Ala Ala Arg Glu Gln 
        275                 280                 285             


Arg Leu Asn Asp Ile His Gln Leu Trp Thr Thr Phe Ala Glu Arg Phe 
    290                 295                 300                 


Gln Ala Leu Ala Ala Glu Lys Thr Arg Asp Ala Ala Leu Ala Tyr Pro 
305                 310                 315                 320 


Gly Tyr Ala Ser Ala Phe Leu Lys Lys Val Trp Ala Asp Ala Val Gly 
                325                 330                 335     


Phe Cys Gly Ser Glu Leu Ile Arg Arg Ser Val Gly Leu Ala His Val 
            340                 345                 350         


Ala Asp Ile Asp Thr Ile Gln Asp Asp Ala Met Arg His Glu Cys Leu 
        355                 360                 365             


Arg Glu Ala Ile Thr Leu Gly Arg Ala Leu Ile Val Leu Ala Glu Arg 
    370                 375                 380                 


Ile Asp Ser Val Asp Glu Leu Leu Ala Arg Val Arg Gln Tyr Ser Leu 
385                 390                 395                 400 


Glu His His His His His His 
                405         


<210> 12
<211> 407
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> MTR kinase AA seq

<400> 12
Met Ser Gln Tyr His Thr Phe Thr Ala Asp Asp Ala Val Ala Tyr Ala 
1               5                   10                  15      


Gln Gln Phe Ala Gly Ile Asp Asn Pro Ser Glu Leu Val Ser Ala Gln 
            20                  25                  30          


Glu Val Gly Asp Gly Asn Leu Asn Leu Val Phe Lys Val Phe Asp Arg 
        35                  40                  45              


Gln Gly Val Ser Arg Ser Ile Val Lys Gln Ala Leu Pro Tyr Pro Arg 
    50                  55                  60                  


Arg Val Gly Pro Ser Trp Pro Leu Thr Leu Asp Arg Ala Arg His Glu 
65                  70                  75                  80  


Ala Gln Thr Leu Val Ala His Tyr Gln His Ser Pro Gln His Thr Val 
                85                  90                  95      


Lys Ile Phe His Phe Asp Pro Glu Leu Ala Val Met Val Met Glu Asp 
            100                 105                 110         


Leu Ser Asp His Arg Ile Trp Arg Gly Glu Leu Ile Ala Asn Val Tyr 
        115                 120                 125             


Tyr Pro Gln Ala Ala Arg Gln Leu Gly Asp Tyr Leu Ala Gln Val Leu 
    130                 135                 140                 


Phe His Thr Ser Asp Phe Tyr Leu His Pro His Glu Lys Lys Arg Gln 
145                 150                 155                 160 


Val Ala Gln Phe Ile Asn Pro Ala Met Cys Gly Ile Ser Glu Asp Leu 
                165                 170                 175     


Val Phe Asn Asp Pro Tyr Gln Ile His Glu Arg Asn Asn Tyr Pro Ala 
            180                 185                 190         


Glu Leu Glu Ala Gln Val Ala Ala Leu Arg Asp Asp Ala Gln Leu Lys 
        195                 200                 205             


Leu Ala Val Ala Ala Leu Lys His Arg Phe Phe Ala His Ala Glu Ala 
    210                 215                 220                 


Leu Leu His Gly Asp Leu His Thr Gly Ser Ile Phe Val Lys Glu Gly 
225                 230                 235                 240 


Ser Leu Lys Val Ile Asp Ala Glu Phe Gly Tyr Phe Gly Pro Ile Gly 
                245                 250                 255     


Phe Asp Ile Gly Thr Ala Ile Gly Asn Leu Leu Leu Asn Tyr Cys Gly 
            260                 265                 270         


Leu Pro Gly Gln Leu Gly Ile Arg Asp Ala Ala Ala Ala Arg Glu Gln 
        275                 280                 285             


Arg Leu Asn Asp Ile His Gln Leu Trp Thr Thr Phe Ala Glu Arg Phe 
    290                 295                 300                 


Gln Ala Leu Ala Ala Glu Lys Thr Arg Asp Ala Ala Leu Arg Tyr Pro 
305                 310                 315                 320 


Gly Tyr Ala Ser Ala Phe Leu Lys Lys Val Trp Ala Asp Ala Val Gly 
                325                 330                 335     


Phe Cys Gly Ser Glu Leu Ile Arg Arg Ser Val Gly Leu Ala His Val 
            340                 345                 350         


Ala Asp Ile Asp Thr Ile Gln Asp Asp Ala Met Arg His Glu Cys Leu 
        355                 360                 365             


Arg Glu Ala Ile Thr Leu Gly Arg Ala Leu Ile Val Leu Ala Glu Arg 
    370                 375                 380                 


Ile Asp Ser Val Asp Glu Leu Leu Ala Arg Val Arg Gln Tyr Ser Leu 
385                 390                 395                 400 


Glu His His His His His His 
                405         


<210> 13
<211> 413
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Acetate kinase

<400> 13
Met Gly Ser His His His His His His Gly Ser Arg Val Leu Asn Ile 
1               5                   10                  15      


Asn Ser Gly Ser Ser Ser Ile Lys Tyr Gln Leu Ile Glu Met Glu Gly 
            20                  25                  30          


Glu Lys Val Leu Cys Lys Gly Ile Ala Glu Arg Ile Gly Ile Glu Gly 
        35                  40                  45              


Ser Arg Leu Val His Arg Val Gly Asp Glu Lys His Val Ile Glu Arg 
    50                  55                  60                  


Glu Leu Pro Asp His Glu Glu Ala Leu Lys Leu Ile Leu Asn Thr Leu 
65                  70                  75                  80  


Val Asp Glu Lys Leu Gly Val Ile Lys Asp Leu Lys Glu Ile Asp Ala 
                85                  90                  95      


Val Gly His Arg Val Val His Gly Gly Glu Arg Phe Lys Glu Ser Val 
            100                 105                 110         


Leu Val Asp Glu Glu Val Leu Lys Ala Ile Glu Glu Val Ser Pro Leu 
        115                 120                 125             


Ala Pro Leu His Asn Pro Ala Asn Leu Met Gly Ile Lys Ala Ala Met 
    130                 135                 140                 


Lys Leu Leu Pro Gly Val Pro Asn Val Gln Val Phe Asp Thr Ala Phe 
145                 150                 155                 160 


His Gln Thr Ile Pro Gln Lys Ala Tyr Leu Tyr Ala Ile Pro Tyr Glu 
                165                 170                 175     


Tyr Tyr Glu Lys Tyr Lys Ile Arg Arg Tyr Gly Phe His Gly Ile Ser 
            180                 185                 190         


His Arg Tyr Val Ser Lys Arg Ala Ala Glu Ile Leu Gly Lys Lys Leu 
        195                 200                 205             


Glu Glu Leu Lys Ile Ile Thr Cys His Ile Gly Asn Gly Ala Ser Val 
    210                 215                 220                 


Ala Ala Val Lys Tyr Gly Lys Cys Val Asp Thr Ser Met Gly Phe Thr 
225                 230                 235                 240 


Pro Leu Glu Gly Leu Val Met Gly Thr Arg Ser Gly Asp Leu Asp Pro 
                245                 250                 255     


Ala Ile Pro Phe Phe Ile Met Glu Lys Glu Gly Ile Ser Pro Gln Glu 
            260                 265                 270         


Met Tyr Asp Ile Leu Asn Lys Lys Ser Gly Val Tyr Gly Leu Ser Lys 
        275                 280                 285             


Gly Phe Ser Ser Asp Met Arg Asp Asn Phe Glu Ala Ala Leu Lys Gly 
    290                 295                 300                 


Asp Glu Trp Cys Lys Leu Val Leu Glu Ile Tyr Asp Tyr Arg Ile Ala 
305                 310                 315                 320 


Lys Tyr Ile Gly Ala Tyr Ala Ala Ala Met Asn Gly Val Asp Ala Ile 
                325                 330                 335     


Val Phe Thr Ala Gly Val Gly Glu Asn Ser Pro Ile Thr Arg Glu Asp 
            340                 345                 350         


Val Cys Lys Tyr Leu Glu Phe Leu Gly Val Lys Leu Asp Lys Gln Lys 
        355                 360                 365             


Asn Glu Glu Thr Ile Leu Gly Lys Glu Gly Ile Ile Ser Thr Pro Asp 
    370                 375                 380                 


Ser Arg Val Lys Val Leu Val Val Pro Thr Asn Glu Glu Leu Met Ile 
385                 390                 395                 400 


Ala Arg Asp Thr Lys Glu Ile Val Glu Lys Ile Gly Arg 
                405                 410             


