                         SEQUENCE LISTING

<110>  The University of Washington
       The University of Western Ontario
 
<120>  Methods And Systems For Quantifying Lipoprotein(A) Using 
       Candidate References

<130>  21-1340-WO

<150>  US 63/127,866
<151>  2020-12-18

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Candidate peptide TPEN

<400>  1

Thr Pro Glu Asn Tyr Pro Asn Ala Gly Leu Thr Arg 
1               5                   10          


<210>  2
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Candidate peptide TPAY

<400>  2

Thr Pro Ala Tyr Tyr Pro Asn Ala Gly Leu Ile Lys 
1               5                   10          


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Candidate peptide GISS

<400>  3

Gly Ile Ser Ser Thr Thr Val Thr Gly Arg 
1               5                   10  


<210>  4
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Candidate peptide LFLE

<400>  4

Leu Phe Leu Glu Pro Thr Gln Ala Asp Ile Ala Leu Leu Lys 
1               5                   10                  


<210>  5
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Candidate peptide GSFS

<400>  5

Gly Ser Phe Ser Thr Thr Val Thr Gly Arg 
1               5                   10  


<210>  6
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  apo(a) fragment TTEY

<400>  6

Thr Thr Glu Tyr Tyr Pro Asn Gly Gly Leu Thr Arg 
1               5                   10          


<210>  7
<211>  1905
<212>  PRT
<213>  Homo sapiens

<400>  7

Glu Gln Ser His Val Val Gln Asp Cys Tyr His Gly Asp Gly Gln Ser 
1               5                   10                  15      


Tyr Arg Gly Thr Tyr Ser Thr Thr Val Thr Gly Arg Thr Cys Gln Ala 
            20                  25                  30          


Trp Ser Ser Met Thr Pro His Gln His Asn Arg Thr Thr Glu Asn Tyr 
        35                  40                  45              


Pro Asn Ala Gly Leu Ile Met Asn Tyr Cys Arg Asn Pro Asp Ala Val 
    50                  55                  60                  


Ala Ala Pro Tyr Cys Tyr Thr Arg Asp Pro Gly Val Arg Trp Glu Tyr 
65                  70                  75                  80  


Cys Asn Leu Thr Gln Cys Ser Asp Ala Glu Gly Thr Ala Val Ala Pro 
                85                  90                  95      


Pro Thr Val Thr Pro Val Pro Ser Leu Glu Ala Pro Ser Glu Gln Ala 
            100                 105                 110         


Pro Thr Glu Gln Arg Pro Gly Val Gln Glu Cys Tyr His Gly Asn Gly 
        115                 120                 125             


Gln Ser Tyr Arg Gly Thr Tyr Ser Thr Thr Val Thr Gly Arg Thr Cys 
    130                 135                 140                 


Gln Ala Trp Ser Ser Met Thr Pro His Ser His Ser Arg Thr Pro Glu 
145                 150                 155                 160 


Tyr Tyr Pro Asn Ala Gly Leu Ile Met Asn Tyr Cys Arg Asn Pro Asp 
                165                 170                 175     


Ala Val Ala Ala Pro Tyr Cys Tyr Thr Arg Asp Pro Gly Val Arg Trp 
            180                 185                 190         


Glu Tyr Cys Asn Leu Thr Gln Cys Ser Asp Ala Glu Gly Thr Ala Val 
        195                 200                 205             


Ala Pro Pro Thr Val Thr Pro Val Pro Ser Leu Glu Ala Pro Ser Glu 
    210                 215                 220                 


Gln Ala Pro Thr Glu Gln Arg Pro Gly Val Gln Glu Cys Tyr His Gly 
225                 230                 235                 240 


Asn Gly Gln Ser Tyr Arg Gly Thr Tyr Ser Thr Thr Val Thr Gly Arg 
                245                 250                 255     


Thr Cys Gln Ala Trp Ser Ser Met Thr Pro His Ser His Ser Arg Thr 
            260                 265                 270         


Pro Glu Tyr Tyr Pro Asn Ala Gly Leu Ile Met Asn Tyr Cys Arg Asn 
        275                 280                 285             


Pro Asp Ala Val Ala Ala Pro Tyr Cys Tyr Thr Arg Asp Pro Gly Val 
    290                 295                 300                 


Arg Trp Glu Tyr Cys Asn Leu Thr Gln Cys Ser Asp Ala Glu Gly Thr 
305                 310                 315                 320 


Ala Val Ala Pro Pro Thr Val Thr Pro Val Pro Ser Leu Glu Ala Pro 
                325                 330                 335     


Ser Glu Gln Ala Pro Thr Glu Gln Arg Pro Gly Val Gln Glu Cys Tyr 
            340                 345                 350         


His Gly Asn Gly Gln Ser Tyr Arg Gly Thr Tyr Ser Thr Thr Val Thr 
        355                 360                 365             


Gly Arg Thr Cys Gln Ala Trp Ser Ser Met Thr Pro His Ser His Ser 
    370                 375                 380                 


Arg Thr Pro Glu Tyr Tyr Pro Asn Ala Gly Leu Ile Met Asn Tyr Cys 
385                 390                 395                 400 


Arg Asn Pro Asp Ala Val Ala Ala Pro Tyr Cys Tyr Thr Arg Asp Pro 
                405                 410                 415     


Gly Val Arg Trp Glu Tyr Cys Asn Leu Thr Gln Cys Ser Asp Ala Glu 
            420                 425                 430         


Gly Thr Ala Val Ala Pro Pro Thr Val Thr Pro Val Pro Ser Leu Glu 
        435                 440                 445             


Ala Pro Ser Glu Gln Ala Pro Thr Glu Gln Arg Pro Gly Val Gln Glu 
    450                 455                 460                 


Cys Tyr His Gly Asn Gly Gln Ser Tyr Arg Gly Thr Tyr Ser Thr Thr 
465                 470                 475                 480 


Val Thr Gly Arg Thr Cys Gln Ala Trp Ser Ser Met Thr Pro His Ser 
                485                 490                 495     


His Ser Arg Thr Pro Glu Tyr Tyr Pro Asn Ala Gly Leu Ile Met Asn 
            500                 505                 510         


Tyr Cys Arg Asn Pro Asp Ala Val Ala Ala Pro Tyr Cys Tyr Thr Arg 
        515                 520                 525             


Asp Pro Gly Val Arg Trp Glu Tyr Cys Asn Leu Thr Gln Cys Ser Asp 
    530                 535                 540                 


Ala Glu Gly Thr Ala Val Ala Pro Pro Thr Val Thr Pro Val Pro Ser 
545                 550                 555                 560 


Leu Glu Ala Pro Ser Glu Gln Ala Pro Thr Glu Gln Arg Pro Gly Val 
                565                 570                 575     


Gln Glu Cys Tyr His Gly Asn Gly Gln Ser Tyr Arg Gly Thr Tyr Ser 
            580                 585                 590         


Thr Thr Val Thr Gly Arg Thr Cys Gln Ala Trp Ser Ser Met Thr Pro 
        595                 600                 605             


His Ser His Ser Arg Thr Pro Glu Tyr Tyr Pro Asn Ala Gly Leu Ile 
    610                 615                 620                 


Met Asn Tyr Cys Arg Asn Pro Asp Ala Val Ala Ala Pro Tyr Cys Tyr 
625                 630                 635                 640 


Thr Arg Asp Pro Gly Val Arg Trp Glu Tyr Cys Asn Leu Thr Gln Cys 
                645                 650                 655     


Ser Asp Ala Glu Gly Thr Ala Val Ala Pro Pro Thr Val Thr Pro Val 
            660                 665                 670         


Pro Ser Leu Glu Ala Pro Ser Glu Gln Ala Pro Thr Glu Gln Arg Pro 
        675                 680                 685             


Gly Val Gln Glu Cys Tyr His Gly Asn Gly Gln Ser Tyr Arg Gly Thr 
    690                 695                 700                 


Tyr Ser Thr Thr Val Thr Gly Arg Thr Cys Gln Ala Trp Ser Ser Met 
705                 710                 715                 720 


Thr Pro His Ser His Ser Arg Thr Pro Glu Tyr Tyr Pro Asn Ala Gly 
                725                 730                 735     


Leu Ile Met Asn Tyr Cys Arg Asn Pro Asp Pro Val Ala Ala Pro Tyr 
            740                 745                 750         


Cys Tyr Thr Arg Asp Pro Ser Val Arg Trp Glu Tyr Cys Asn Leu Thr 
        755                 760                 765             


Gln Cys Ser Asp Ala Glu Gly Thr Ala Val Ala Pro Pro Thr Ile Thr 
    770                 775                 780                 


Pro Ile Pro Ser Leu Glu Ala Pro Ser Glu Gln Ala Pro Thr Glu Gln 
785                 790                 795                 800 


Arg Pro Gly Val Gln Glu Cys Tyr His Gly Asn Gly Gln Ser Tyr Gln 
                805                 810                 815     


Gly Thr Tyr Phe Ile Thr Val Thr Gly Arg Thr Cys Gln Ala Trp Ser 
            820                 825                 830         


Ser Met Thr Pro His Ser His Ser Arg Thr Pro Ala Tyr Tyr Pro Asn 
        835                 840                 845             


Ala Gly Leu Ile Lys Asn Tyr Cys Arg Asn Pro Asp Pro Val Ala Ala 
    850                 855                 860                 


Pro Trp Cys Tyr Thr Thr Asp Pro Ser Val Arg Trp Glu Tyr Cys Asn 
865                 870                 875                 880 


Leu Thr Arg Cys Ser Asp Ala Glu Trp Thr Ala Phe Val Pro Pro Asn 
                885                 890                 895     


Val Ile Leu Ala Pro Ser Leu Glu Ala Phe Phe Glu Gln Ala Leu Thr 
            900                 905                 910         


Glu Glu Thr Pro Gly Val Gln Asp Cys Tyr Tyr His Tyr Gly Gln Ser 
        915                 920                 925             


Tyr Arg Gly Thr Tyr Ser Thr Thr Val Thr Gly Arg Thr Cys Gln Ala 
    930                 935                 940                 


Trp Ser Ser Met Thr Pro His Gln His Ser Arg Thr Pro Glu Asn Tyr 
945                 950                 955                 960 


Pro Asn Ala Gly Leu Thr Arg Asn Tyr Cys Arg Asn Pro Asp Ala Glu 
                965                 970                 975     


Ile Arg Pro Trp Cys Tyr Thr Met Asp Pro Ser Val Arg Trp Glu Tyr 
            980                 985                 990         


Cys Asn Leu Thr Gln Cys Leu Val  Thr Glu Ser Ser Val  Leu Ala Thr 
        995                 1000                 1005             


Leu Thr  Val Val Pro Asp Pro  Ser Thr Glu Ala Ser  Ser Glu Glu 
    1010                 1015                 1020             


Ala Pro  Thr Glu Gln Ser Pro  Gly Val Gln Asp Cys  Tyr His Gly 
    1025                 1030                 1035             


Asp Gly  Gln Ser Tyr Arg Gly  Ser Phe Ser Thr Thr  Val Thr Gly 
    1040                 1045                 1050             


Arg Thr  Cys Gln Ser Trp Ser  Ser Met Thr Pro His  Trp His Gln 
    1055                 1060                 1065             


Arg Thr  Thr Glu Tyr Tyr Pro  Asn Gly Gly Leu Thr  Arg Asn Tyr 
    1070                 1075                 1080             


Cys Arg  Asn Pro Asp Ala Glu  Ile Ser Pro Trp Cys  Tyr Thr Met 
    1085                 1090                 1095             


Asp Pro  Asn Val Arg Trp Glu  Tyr Cys Asn Leu Thr  Gln Cys Pro 
    1100                 1105                 1110             


Val Thr  Glu Ser Ser Val Leu  Ala Thr Ser Thr Ala  Val Ser Glu 
    1115                 1120                 1125             


Gln Ala  Pro Thr Glu Gln Ser  Pro Thr Val Gln Asp  Cys Tyr His 
    1130                 1135                 1140             


Gly Asp  Gly Gln Ser Tyr Arg  Gly Ser Phe Ser Thr  Thr Val Thr 
    1145                 1150                 1155             


Gly Arg  Thr Cys Gln Ser Trp  Ser Ser Met Thr Pro  His Trp His 
    1160                 1165                 1170             


Gln Arg  Thr Thr Glu Tyr Tyr  Pro Asn Gly Gly Leu  Thr Arg Asn 
    1175                 1180                 1185             


Tyr Cys  Arg Asn Pro Asp Ala  Glu Ile Arg Pro Trp  Cys Tyr Thr 
    1190                 1195                 1200             


Met Asp  Pro Ser Val Arg Trp  Glu Tyr Cys Asn Leu  Thr Gln Cys 
    1205                 1210                 1215             


Pro Val  Met Glu Ser Thr Leu  Leu Thr Thr Pro Thr  Val Val Pro 
    1220                 1225                 1230             


Val Pro  Ser Thr Glu Leu Pro  Ser Glu Glu Ala Pro  Thr Glu Asn 
    1235                 1240                 1245             


Ser Thr  Gly Val Gln Asp Cys  Tyr Arg Gly Asp Gly  Gln Ser Tyr 
    1250                 1255                 1260             


Arg Gly  Thr Leu Ser Thr Thr  Ile Thr Gly Arg Thr  Cys Gln Ser 
    1265                 1270                 1275             


Trp Ser  Ser Met Thr Pro His  Trp His Arg Arg Ile  Pro Leu Tyr 
    1280                 1285                 1290             


Tyr Pro  Asn Ala Gly Leu Thr  Arg Asn Tyr Cys Arg  Asn Pro Asp 
    1295                 1300                 1305             


Ala Glu  Ile Arg Pro Trp Cys  Tyr Thr Met Asp Pro  Ser Val Arg 
    1310                 1315                 1320             


Trp Glu  Tyr Cys Asn Leu Thr  Arg Cys Pro Val Thr  Glu Ser Ser 
    1325                 1330                 1335             


Val Leu  Thr Thr Pro Thr Val  Ala Pro Val Pro Ser  Thr Glu Ala 
    1340                 1345                 1350             


Pro Ser  Glu Gln Ala Pro Pro  Glu Lys Ser Pro Val  Val Gln Asp 
    1355                 1360                 1365             


Cys Tyr  His Gly Asp Gly Arg  Ser Tyr Arg Gly Ile  Ser Ser Thr 
    1370                 1375                 1380             


Thr Val  Thr Gly Arg Thr Cys  Gln Ser Trp Ser Ser  Met Ile Pro 
    1385                 1390                 1395             


His Trp  His Gln Arg Thr Pro  Glu Asn Tyr Pro Asn  Ala Gly Leu 
    1400                 1405                 1410             


Thr Glu  Asn Tyr Cys Arg Asn  Pro Asp Ser Gly Lys  Gln Pro Trp 
    1415                 1420                 1425             


Cys Tyr  Thr Thr Asp Pro Cys  Val Arg Trp Glu Tyr  Cys Asn Leu 
    1430                 1435                 1440             


Thr Gln  Cys Ser Glu Thr Glu  Ser Gly Val Leu Glu  Thr Pro Thr 
    1445                 1450                 1455             


Val Val  Pro Val Pro Ser Met  Glu Ala His Ser Glu  Ala Ala Pro 
    1460                 1465                 1470             


Thr Glu  Gln Thr Pro Val Val  Arg Gln Cys Tyr His  Gly Asn Gly 
    1475                 1480                 1485             


Gln Ser  Tyr Arg Gly Thr Phe  Ser Thr Thr Val Thr  Gly Arg Thr 
    1490                 1495                 1500             


Cys Gln  Ser Trp Ser Ser Met  Thr Pro His Arg His  Gln Arg Thr 
    1505                 1510                 1515             


Pro Glu  Asn Tyr Pro Asn Asp  Gly Leu Thr Met Asn  Tyr Cys Arg 
    1520                 1525                 1530             


Asn Pro  Asp Ala Asp Thr Gly  Pro Trp Cys Phe Thr  Met Asp Pro 
    1535                 1540                 1545             


Ser Ile  Arg Trp Glu Tyr Cys  Asn Leu Thr Arg Cys  Ser Asp Thr 
    1550                 1555                 1560             


Glu Gly  Thr Val Val Ala Pro  Pro Thr Val Ile Gln  Val Pro Ser 
    1565                 1570                 1575             


Leu Gly  Pro Pro Ser Glu Gln  Asp Cys Met Phe Gly  Asn Gly Lys 
    1580                 1585                 1590             


Gly Tyr  Arg Gly Lys Lys Ala  Thr Thr Val Thr Gly  Thr Pro Cys 
    1595                 1600                 1605             


Gln Glu  Trp Ala Ala Gln Glu  Pro His Arg His Ser  Thr Phe Ile 
    1610                 1615                 1620             


Pro Gly  Thr Asn Lys Trp Ala  Gly Leu Glu Lys Asn  Tyr Cys Arg 
    1625                 1630                 1635             


Asn Pro  Asp Gly Asp Ile Asn  Gly Pro Trp Cys Tyr  Thr Met Asn 
    1640                 1645                 1650             


Pro Arg  Lys Leu Phe Asp Tyr  Cys Asp Ile Pro Leu  Cys Ala Ser 
    1655                 1660                 1665             


Ser Ser  Phe Asp Cys Gly Lys  Pro Gln Val Glu Pro  Lys Lys Cys 
    1670                 1675                 1680             


Pro Gly  Ser Ile Val Gly Gly  Cys Val Ala His Pro  His Ser Trp 
    1685                 1690                 1695             


Pro Trp  Gln Val Ser Leu Arg  Thr Arg Phe Gly Lys  His Phe Cys 
    1700                 1705                 1710             


Gly Gly  Thr Leu Ile Ser Pro  Glu Trp Val Leu Thr  Ala Ala His 
    1715                 1720                 1725             


Cys Leu  Lys Lys Ser Ser Arg  Pro Ser Ser Tyr Lys  Val Ile Leu 
    1730                 1735                 1740             


Gly Ala  His Gln Glu Val Asn  Leu Glu Ser His Val  Gln Glu Ile 
    1745                 1750                 1755             


Glu Val  Ser Arg Leu Phe Leu  Glu Pro Thr Gln Ala  Asp Ile Ala 
    1760                 1765                 1770             


Leu Leu  Lys Leu Ser Arg Pro  Ala Val Ile Thr Asp  Lys Val Met 
    1775                 1780                 1785             


Pro Ala  Cys Leu Pro Ser Pro  Asp Tyr Met Val Thr  Ala Arg Thr 
    1790                 1795                 1800             


Glu Cys  Tyr Ile Thr Gly Trp  Gly Glu Thr Gln Gly  Thr Phe Gly 
    1805                 1810                 1815             


Thr Gly  Leu Leu Lys Glu Ala  Gln Leu Leu Val Ile  Glu Asn Glu 
    1820                 1825                 1830             


Val Cys  Asn His Tyr Lys Tyr  Ile Cys Ala Glu His  Leu Ala Arg 
    1835                 1840                 1845             


Gly Thr  Asp Ser Cys Gln Gly  Asp Ser Gly Gly Pro  Leu Val Cys 
    1850                 1855                 1860             


Phe Glu  Lys Asp Lys Tyr Ile  Leu Gln Gly Val Thr  Ser Trp Gly 
    1865                 1870                 1875             


Leu Gly  Cys Ala Arg Pro Asn  Lys Pro Gly Val Tyr  Ala Arg Val 
    1880                 1885                 1890             


Ser Arg  Phe Val Thr Trp Ile  Glu Gly Met Met Arg  
    1895                 1900                 1905 


<210>  8
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isotope heavy peptide LFLE


<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  heavy isotope at 13C6 and 15N2

<400>  8

Leu Phe Leu Glu Pro Thr Gln Ala Asp Ile Ala Leu Leu Lys 
1               5                   10                  


<210>  9
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isotope heavy peptide GISS


<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  heavy isotope at 13C6 and 15N4

<400>  9

Gly Ile Ser Ser Thr Thr Val Thr Gly Arg 
1               5                   10  


<210>  10
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isotope heavy peptide TPAY


<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  heavy isotope at 13C6 and 15N2

<400>  10

Thr Pro Ala Tyr Tyr Pro Asn Ala Gly Leu Ile Lys 
1               5                   10          


<210>  11
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  isotope heavy peptide TPEN


<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  heavy isotope at 13C6 and 15N4

<400>  11

Thr Pro Glu Asn Tyr Pro Asn Ala Gly Leu Thr Arg 
1               5                   10          


