                         SEQUENCE LISTING

<110>  Vrije Universiteit Brussel
 
<120>  CARDIOMYOCYTE-DERIVED NUCLEIC ACID REGULATORY ELEMENTS AND 
       METHODS AND USE THEREOF

<130>  VUB-087-PCT

<150>  20216054.5
<151>  2020-12-21

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  482
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CARD-CRE3

<400>  1
cattttatta aacactctaa accagatcca gtactaagtg tttcatatga atttttttca       60

tctaaccatc aacaaacttt taaggtaagc actatcatcc tctcctctta cagatgagga      120

tataaagctt agaaaaacaa tgtgcaaaaa tgctactcat taagcacaga tgtgaaaagg      180

aaaaagaaaa tgccctgaag gcagctcgca tgagtataat gtctgagaag tttgttgata      240

attgacaaag aatgaagttt tctcaatcac aagccttgta gaaattgtgg cgtcaagttg      300

tgtcactgtc aataccaggt gattagtcaa gtgattacat aatggtctcc ctcagaacca      360

aaggtatggg caagacaact ggctaaagaa ttttgtgggt gacttctttc acatgagatt      420

aatttatcaa aagatgtcac acaggactct gccaactgac acgctaccag tttccatctg      480

tt                                                                     482


<210>  2
<211>  355
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CARD-CRE8

<400>  2
cttatctccc tccactgctg ctacttaatt tgtttcctct ctgccacccc tcaccagcat       60

gtcagacata caaaacaagg gatttttgtg tgcttggcac acagtagatg cacaataaat      120

gttgaagggc tgaactaatt tgggtttgag tcatagggag cttgggggat gtgggtgatt      180

ggatagattc tggagacttt aggggactgg gccgggggaa atgcggcctc taagctctcc      240

gctgaggcgg cttggaagga atagtgactg acgtggaggt gggggaggtg gctggcccgg      300

gcgaggccca gggagaggga gaggaggcgg gtgggagagg aggagggtgt atctc           355


<210>  3
<211>  1640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CARD-CRE11

<400>  3
aactgagttg tgtgacattc acgtggcccc ttcagttagc accgcacggt tggttaagag       60

gagggtttta ttcaggcata aatattttga aatatttgta atcaaatccc acgtagtata      120

gacaacctcc cagatggtgg aggggaaaca caccaccccc ctgactggga gagaaaagac      180

cgcggttttc tttcctgcca cttgtcctcc tccgggagcc tcggtctcct ctctgtggga      240

cagggcagcc tcccctggct cctcctggtg caggatcgat gaggctcttg cccacagcct      300

ttgtcccccg acttcctacc acctggtgtc ctcaccccat ctgggccccg agcacacagc      360

ctctggtcct ggtttccact gaaaatgtaa actatggtcc acgccacatt ctccagtact      420

cctgtgctat tgtgaaagta aaagacaaaa atacacgtgc ttggcagccc gagagcctgg      480

gtgagattat tgagaataat gcggtggctc tggcttaaaa aataaaagcc ggaggatcta      540

ccaccagctc tgaacacaca cccgtgacac aaactgactc atgtgcaatt gcccattagt      600

ggcaggtctt gccatttttc gtttttttct gagtcgagat cagctgcggc tggggagaac      660

attagttctg tctgtcggct ccatgagtaa gcagaggaaa gtctgactac agaggcatct      720

tctcaaatgt ccatgaaagg ctgcaagccc gagagtaacg cccctgctgc cctgagtggt      780

ccacgtggtg tccagagtgt gagaaagcca cagagagctt ttccacacgg agccacttaa      840

ggcctgcctc agcctgagaa cacttgggca gaaaaggaca gcacggcttc ctcctcctcc      900

aggccgggcc tacaagcacc cacagttccg acagagaaag tctcttctta atggtacgca      960

tgtgtcttac tgtagctccc agttagtccc ctaagcgtga accaccaccc acccatgctg     1020

ctcccctccc accatctctc cataaacagg acccaacgca gccaaaaact catggtcatg     1080

tgaccttggc atcagcttgc tcgttaggaa ccgtgcctgg aggtgctctg agcactgact     1140

gccaacctca gcagggccct ttgtggtgtt gaggtgtggt gtccgcaggg cagcctgcag     1200

cctgctgggg tccagcctgg ggcctcaggt cctgggaacc ctggttgagg ctgggctgcc     1260

tcagtgcccc tccgcagcct gctgcaccca agcactctga gaaaaggagc aatcacccaa     1320

cttcagtctg aaaatgagaa tgttgagaag ggcatgatga ggggaagcag accagagccc     1380

accggcaagg gtagaacgtc agccccgccc tagtgggaca gacaagagag aagcctcctc     1440

acaccaggag gcagtatagc cggagccttc cctccagggc tctcaggaga gcctgtgctg     1500

gttcatgaaa aagtgaggcc tctgggcttt gctgctgcag agtgaggtca gagcccacag     1560

gccccacaga tcatcctgcc tgacaagcac aggaggcacc actgcttgaa ttcagagggc     1620

agctccgagg gtcctaggtc                                                 1640


<210>  4
<211>  495
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CARD-CRE12

<400>  4
gacattcacg tggccccttc agttagcacc gcacggttgg ttaagaggag ggttttattc       60

aggcataaat attttgaaat atttgtaatc aaatcccacg tagtatagac aacctcccag      120

atggtggagg ggaaacacac cacccccctg actgggagag aaaagaccgc ggttttcttt      180

cctgccactt gtcctcctcc gggagcctcg gtctcctctc tgtgggacag ggcagcctcc      240

cctggctcct cctggtgcag gatcgatgag gctcttgccc acagcctttg tcccccgact      300

tcctaccacc tggtgtcctc accccatctg ggccccgagc acacagcctc tggtcctggt      360

ttccactgaa aatgtaaact atggtccacg ccacattctc cagtactcct gtgctattgt      420

gaaagtaaaa gacaaaaata cacgtgcttg gcagcccgag agcctgggtg agattattga      480

gaataatgcg gtggc                                                       495


<210>  5
<211>  548
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CARD-CRE14

<400>  5
ccccacccca ccccttactt gtttttcctt ctactcattt tctcctccct ccctctcccc       60

tctccccaca ctagaaccat cagggccctc accagggcat tcagttcagg ttctgggtca      120

gctctcacag ctctaccagt cccaccccca tccccaggaa aaactgctcc ttatttggag      180

tcacaaaaat atttaacaga gatctaactg accactaaaa attcctcctt taaaacaaac      240

acctaatcaa ctattttccc ccaagttata tggaaaaaca gctgcaatta gaacttgatt      300

ctcactttaa gaaagaaaga ttcttgtttg gttttctcca ctttcatttt ttgtttctag      360

gtccagggcc tcccaccaaa tgctgacggc tgcctgcttc aaaccctgcc acatcagcag      420

ggagggagca cagcggcatt tggtatttgc atcagtttcc aggaatgctt ttcaagttat      480

cagttcactc tgctgccttt agcagagacg ttttccctct aagtttatag atgtctgctt      540

caatttac                                                               548


<210>  6
<211>  844
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CARD-CRE16

<400>  6
agctgagatc acgccactgc actccagcct gggcgacaga gcgagactcc atctcaaaaa       60

taaatatata aataaataaa aataaaaaag agaaaggaca gggtgcacag agccaggacc      120

ccaccccctc ctggtctgga ccaccctggg ccatctccta agcttgacac tctcaggcct      180

gaggctgtca ggccagggcg ctttgactat cccctgcctg cccctgaacg cggccaagcc      240

cgaggcctta gccaagccct gcagcctcag gcttggcgcc cggcccagcc tttctttagc      300

cttcccgagg agccagcgag agagcgctgc agcagccgca gtccttgctt ccggggaagg      360

cccgggtggg gccagggcct gtggttaagt cagtggccct ctgcagacgc gcacgcagca      420

ggagccacgc cctggtccct gcccgggagt ggcctggtct cccccagctc tgattccctg      480

aaggaccctg cacctcctca gccatcttct ctgggcaccc cctgagccca gcctgctggc      540

cacaggcccc ttcccgccaa cttgtgggcc tagctcatca tcaaactatt ttccactggc      600

ttcccccaat ctctgccaca gcacaatttt ggtggggagg tggagaggga tgaaggagaa      660

taggaggtgg agcttggact ctggccgggg gcggcggggt gagggggggc agcagctcgc      720

cactctgatt ggtcacctct gctccaaaac tggcttcaaa attccacgga ctccgccccc      780

agtggccccc agccctatcc tgacttgcaa ttggctgaac tttcaggggg cggggctcac      840

ccgg                                                                   844


<210>  7
<211>  300
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CARD-CRE17

<400>  7
ctgctggcca caggcccctt cccgccaact tgtgggccta gctcatcatc aaactatttt       60

ccactggctt cccccaatct ctgccacagc acaattttgg tggggaggtg gagagggatg      120

aaggagaata ggaggtggag cttggactct ggccgggggc ggcggggtga gggggggcag      180

cagctcgcca ctctgattgg tcacctctgc tccaaaactg gcttcaaaat tccacggact      240

ccgcccccag tggcccccag ccctatcctg acttgcaatt ggctgaactt tcagggggcg      300


<210>  8
<211>  1478
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CARD-CRE20

<400>  8
aagggcgcga gtgagccgtg tcgggccggc cggcgcctta aacccgccgc cccgcccggc       60

ctgtccgcgg gaaccacacc ccggggcggt gagagcacgg gggccccgcc cgtgccgccc      120

tcccggcccc gcggggcctg cgcgcgcgct cctgctggcg tcggggggtg agcgggcacc      180

cggcgtgacc ggcccatggg cgctcggtct cacgcccgct tcgcagagct caccccgcca      240

gctcctgctc ggtccccagt cccctgcccc gagccctcag ggatcgcacc gcagtgcgag      300

cggctcttcc accctctggt ttctccccgt ttccggtcaa tatatttaaa cttcttaaca      360

accacggctt cgagacgatc cagggtttca tctattaaat cctccgtggg cctgagacac      420

ctgagtgctc attactctgt ggtttgggaa gctctcttaa gaacaagctg ttaaaaaccc      480

ccacttgtgg actgtgtccc cgctggccac aggcctgctg tggtcttggg tgtcaagcac      540

taaaaggaca aaccgttgcc aaccacttcc ctttctggaa cctcttaact gcctgcacct      600

ccctccccgc tttgtctccc agtggcattc aacaagactc tgtgcttttt ttgtgctgag      660

ccgttttcag tgagtactgg aatccgtgtg agagctgtgc ccttgttttg agttcttgct      720

gaactgctcg ccctccagca agcctctcgg gagaacagag cgcagctgag aggagcaggg      780

gtcccagctc cttcctcgtt ggagctgcca cggaggcacc taaagcgccc ccagtcttca      840

ccagcattcc aggaggtttt tgatataaac ggtggactct tcctcagtct ttctgcgaag      900

tgctctgcaa actcaacttg aaagcctctc ttcccaggaa cactggcctc tccaatgcag      960

acatggaagt tgctttcact ttttaactga atgtgtaaca ttgcagcgtg cattattctg     1020

catgtcacat tgtggttctt ttgtgttctt ttttcttagc ataaatccag ggatttacag     1080

ggttgctgac tgaaagccag cagttcagtt cataatccca ttagcagtgt aaagaactca     1140

ttctcaacag actcttacaa ggataattat tgcaaatttt ttgaaaatta aatgaccaca     1200

aaatcggggg gtggagagtg gggaaacatc ctccaatcca gaaaaaccta gcactgcttt     1260

ttggcctctc cttagtgtat tatagacaag tggagcattt tttttcccct caaaggtcat     1320

atgttgacct gacagttgtt taaaaaacaa tgaaaaatct ttacagcaga gtttccctaa     1380

atatttttgt ctttgttcca tcctaagaga tgcagtttac atgacccatt tgttttcttg     1440

aaaaggaaac aaatgcagag tgcactctat ttcctatt                             1478


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Luciferase forward primer

<400>  9
cccaccgtcg tattcgtgag                                                   20


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Luciferase reverse primer

<400>  10
tcagggcgat ggttttgtcc c                                                 21


<210>  11
<211>  334
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SPc5-12 promoter

<400>  11
tggccaccgc cttcggcacc atcctcacga cacccaaata tggcgacggg tgaggaatgg       60

tggggagtta tttttagagc ggtgaggaag gtgggcaggc agcaggtgtt ggcgctctaa      120

aaataactcc cgggagttat ttttagagcg gaggaatggt ggacacccaa atatggcgac      180

ggttcctcac ccgtcgccat atttgggtgt ccgccctcgg ccggggccgc attcctgggg      240

gccgggcggt gctcccgccc gcctcgataa aaggctccgg ggccggcggc ggcccacgag      300

ctacccggag gagcgggagg cgccaagctc taga                                  334


<210>  12
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MVM intron

<400>  12
aagaggtaag ggtttaaggg atggttggtt ggtggggtat taatgtttaa ttacctggag       60

cacctgcctg aaatcacttt ttttcaggtt gg                                     92


<210>  13
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pA signal

<400>  13
aataaaagat ctttattttc attagatctg tgtgttggtt ttttgtgtg                   49


<210>  14
<211>  567
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hMLC promoter

<400>  14
atgagccaca gtgccagcct tcatggttat tttaaagatg gtggtcgggg aggcttcact       60

caggagatga catatgagca aagatgcagt gaaggaggtg aaggaaggag ccgtgcgatg      120

actgacagaa agacattcca ggtagagggc acacaggtgc aaagaccctg aggccagatc      180

caggctgata aaacagagca ttttagcagt ctcctctccc tgccattttt tttctcaaaa      240

ttgacaagca caagtgtccc cggcccaagc accgcagaga gcgcgcagca tctctccccg      300

tgaccatgac ccagctactg cctctttaac cttgaatgcc tttttggggg ctcacgtgtc      360

acccagtggc gagtgagcca cccttacttc agaagaacgg catggggtgg gggggcctta      420

ggtggtgccc gcctcaccta tgactgccaa aagcggtcat ggggttattt ttaaacatgg      480

ggaggaagta tttattgttc ctgggctgca gagagctggg cggagtgtgg aattcttctc      540

gggaggcagt gctgggtcct ttccacc                                          567


<210>  15
<211>  663
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CMV promoter

<400>  15
acgcgtggag ctagttatta atagtaatca attacggggt cattagttca tagcccatat       60

atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac      120

ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgtcaat agggactttc      180

cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg      240

tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat      300

tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc      360

atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt      420

gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttgcacc      480

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      540

gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg      600

cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc      660

tcc                                                                    663


