                         SEQUENCE LISTING

<110>  ʦѧ
       ϺҫƼ޹˾
 
<120>  һϸел༭ķƷӦ

<130>  P214010674CF

<150>  CN202011606753.9
<151>  2020-12-28

<160>  47    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgHBGsense

<400>  1
cttgaccaat agccttgaca                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgHBGsite1

<400>  2
cttgtcaagg ctattggtca                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgHBGnspacer1

<400>  3
atgcaaatat ctgtctgaaa                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgHBGnspacer2

<400>  4
atatttgcat tgagatagtg                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgHBGnspacer3

<400>  5
tatttgcatt gagatagtgt                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgHBGnspacer4

<400>  6
atttgcattg agatagtgtg                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgHBGnspacer5

<400>  7
gcattgagat agtgtgggga                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgHBGnspacer6

<400>  8
gtggggaagg ggcccccaag                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sg1617

<400>  9
ctaacagttg cttttatcac                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sg1618

<400>  10
ttgcttttat cacaggctcc                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sg1619

<400>  11
ttttatcaca ggctccagga                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sg1620

<400>  12
tttatcacag gctccaggaa                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sg1621

<400>  13
cacaggctcc aggaagggtt                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  55_sgRNA1

<400>  14
cactgatagg ggtcgcggta                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  55_sgRNA2

<400>  15
gcactgatag gggtcgcggt                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TFR2_Nontrans_sg1

<400>  16
acaagcatgg agcggctttg                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TFR2_Nontrans_sg2

<400>  17
cagctccatg gggcagaagt                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TFR2_Nontrans_sg3

<400>  18
ggccatgttc ctgcagttcc                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TFR2_Nontrans_sg4

<400>  19
cgggatggcc gctctgactc                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TFR2_Nontrans_sg5

<400>  20
ctccatggtg agcaacggta                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TFR2_ABEskip_sg1

<400>  21
gcttaggcaa accagccttc                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TFR2_ABEskip_sg2

<400>  22
tccccaggtg accaatgctc                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TFR2_ABEskip_sg3

<400>  23
ctgcaggtgc acctgggaac                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TFR2_ABEskip_sg4

<400>  24
caggatccgg cccctaccca                                                   20


<210>  25
<211>  1610
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ABE8e

<400>  25

His His His His His His Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu 
1               5                   10                  15      


Ser Pro Lys Lys Lys Arg Lys Val Ser Glu Val Glu Phe Ser His Glu 
            20                  25                  30          


Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu 
        35                  40                  45              


Arg Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile 
    50                  55                  60                  


Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala His 
65                  70                  75                  80  


Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr 
                85                  90                  95      


Arg Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met 
            100                 105                 110         


Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly 
        115                 120                 125             


Val Arg Asn Ser Lys Arg Gly Ala Ala Gly Ser Leu Met Asn Val Leu 
    130                 135                 140                 


Asn Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu 
145                 150                 155                 160 


Ala Asp Glu Cys Ala Ala Leu Leu Cys Asp Phe Tyr Arg Met Pro Arg 
                165                 170                 175     


Gln Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Ile Asn Ser Gly 
            180                 185                 190         


Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu 
        195                 200                 205             


Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Asp Lys 
    210                 215                 220                 


Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala 
225                 230                 235                 240 


Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu 
                245                 250                 255     


Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu 
            260                 265                 270         


Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys Arg Thr 
        275                 280                 285             


Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln 
    290                 295                 300                 


Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe Phe His 
305                 310                 315                 320 


Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg 
                325                 330                 335     


His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys 
            340                 345                 350         


Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp 
        355                 360                 365             


Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys 
    370                 375                 380                 


Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser 
385                 390                 395                 400 


Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu 
                405                 410                 415     


Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile 
            420                 425                 430         


Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala 
        435                 440                 445             


Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala 
    450                 455                 460                 


Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala 
465                 470                 475                 480 


Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu 
                485                 490                 495     


Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu 
            500                 505                 510         


Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg 
        515                 520                 525             


Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys 
    530                 535                 540                 


Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val 
545                 550                 555                 560 


Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser 
                565                 570                 575     


Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu 
            580                 585                 590         


Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu 
        595                 600                 605             


Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg 
    610                 615                 620                 


Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly Glu Leu 
625                 630                 635                 640 


His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp 
                645                 650                 655     


Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr 
            660                 665                 670         


Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg 
        675                 680                 685             


Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp 
    690                 695                 700                 


Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp 
705                 710                 715                 720 


Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr 
                725                 730                 735     


Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr 
            740                 745                 750         


Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala 
        755                 760                 765             


Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln 
    770                 775                 780                 


Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu 
785                 790                 795                 800 


Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His 
                805                 810                 815     


Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn Glu Glu 
            820                 825                 830         


Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu Phe Glu 
        835                 840                 845             


Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala His Leu Phe 
    850                 855                 860                 


Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr Thr Gly Trp 
865                 870                 875                 880 


Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys Gln Ser 
                885                 890                 895     


Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe Ala Asn Arg 
            900                 905                 910         


Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe Lys Glu Asp 
        915                 920                 925             


Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu His Glu His 
    930                 935                 940                 


Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln 
945                 950                 955                 960 


Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly Arg His Lys 
                965                 970                 975     


Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Gln 
            980                 985                 990         


Lys Gly Gln Lys Asn Ser Arg Glu  Arg Met Lys Arg Ile  Glu Glu Gly 
        995                 1000                 1005             


Ile Lys  Glu Leu Gly Ser Gln  Ile Leu Lys Glu His  Pro Val Glu 
    1010                 1015                 1020             


Asn Thr  Gln Leu Gln Asn Glu  Lys Leu Tyr Leu Tyr  Tyr Leu Gln 
    1025                 1030                 1035             


Asn Gly  Arg Asp Met Tyr Val  Asp Gln Glu Leu Asp  Ile Asn Arg 
    1040                 1045                 1050             


Leu Ser  Asp Tyr Asp Val Asp  His Ile Val Pro Gln  Ser Phe Leu 
    1055                 1060                 1065             


Lys Asp  Asp Ser Ile Asp Asn  Lys Val Leu Thr Arg  Ser Asp Lys 
    1070                 1075                 1080             


Asn Arg  Gly Lys Ser Asp Asn  Val Pro Ser Glu Glu  Val Val Lys 
    1085                 1090                 1095             


Lys Met  Lys Asn Tyr Trp Arg  Gln Leu Leu Asn Ala  Lys Leu Ile 
    1100                 1105                 1110             


Thr Gln  Arg Lys Phe Asp Asn  Leu Thr Lys Ala Glu  Arg Gly Gly 
    1115                 1120                 1125             


Leu Ser  Glu Leu Asp Lys Ala  Gly Phe Ile Lys Arg  Gln Leu Val 
    1130                 1135                 1140             


Glu Thr  Arg Gln Ile Thr Lys  His Val Ala Gln Ile  Leu Asp Ser 
    1145                 1150                 1155             


Arg Met  Asn Thr Lys Tyr Asp  Glu Asn Asp Lys Leu  Ile Arg Glu 
    1160                 1165                 1170             


Val Lys  Val Ile Thr Leu Lys  Ser Lys Leu Val Ser  Asp Phe Arg 
    1175                 1180                 1185             


Lys Asp  Phe Gln Phe Tyr Lys  Val Arg Glu Ile Asn  Asn Tyr His 
    1190                 1195                 1200             


His Ala  His Asp Ala Tyr Leu  Asn Ala Val Val Gly  Thr Ala Leu 
    1205                 1210                 1215             


Ile Lys  Lys Tyr Pro Lys Leu  Glu Ser Glu Phe Val  Tyr Gly Asp 
    1220                 1225                 1230             


Tyr Lys  Val Tyr Asp Val Arg  Lys Met Ile Ala Lys  Ser Glu Gln 
    1235                 1240                 1245             


Glu Ile  Gly Lys Ala Thr Ala  Lys Tyr Phe Phe Tyr  Ser Asn Ile 
    1250                 1255                 1260             


Met Asn  Phe Phe Lys Thr Glu  Ile Thr Leu Ala Asn  Gly Glu Ile 
    1265                 1270                 1275             


Arg Lys  Arg Pro Leu Ile Glu  Thr Asn Gly Glu Thr  Gly Glu Ile 
    1280                 1285                 1290             


Val Trp  Asp Lys Gly Arg Asp  Phe Ala Thr Val Arg  Lys Val Leu 
    1295                 1300                 1305             


Ser Met  Pro Gln Val Asn Ile  Val Lys Lys Thr Glu  Val Gln Thr 
    1310                 1315                 1320             


Gly Gly  Phe Ser Lys Glu Ser  Ile Leu Pro Lys Arg  Asn Ser Asp 
    1325                 1330                 1335             


Lys Leu  Ile Ala Arg Lys Lys  Asp Trp Asp Pro Lys  Lys Tyr Gly 
    1340                 1345                 1350             


Gly Phe  Asp Ser Pro Thr Val  Ala Tyr Ser Val Leu  Val Val Ala 
    1355                 1360                 1365             


Lys Val  Glu Lys Gly Lys Ser  Lys Lys Leu Lys Ser  Val Lys Glu 
    1370                 1375                 1380             


Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser Ser Phe  Glu Lys Asn 
    1385                 1390                 1395             


Pro Ile  Asp Phe Leu Glu Ala  Lys Gly Tyr Lys Glu  Val Lys Lys 
    1400                 1405                 1410             


Asp Leu  Ile Ile Lys Leu Pro  Lys Tyr Ser Leu Phe  Glu Leu Glu 
    1415                 1420                 1425             


Asn Gly  Arg Lys Arg Met Leu  Ala Ser Ala Gly Glu  Leu Gln Lys 
    1430                 1435                 1440             


Gly Asn  Glu Leu Ala Leu Pro  Ser Lys Tyr Val Asn  Phe Leu Tyr 
    1445                 1450                 1455             


Leu Ala  Ser His Tyr Glu Lys  Leu Lys Gly Ser Pro  Glu Asp Asn 
    1460                 1465                 1470             


Glu Gln  Lys Gln Leu Phe Val  Glu Gln His Lys His  Tyr Leu Asp 
    1475                 1480                 1485             


Glu Ile  Ile Glu Gln Ile Ser  Glu Phe Ser Lys Arg  Val Ile Leu 
    1490                 1495                 1500             


Ala Asp  Ala Asn Leu Asp Lys  Val Leu Ser Ala Tyr  Asn Lys His 
    1505                 1510                 1515             


Arg Asp  Lys Pro Ile Arg Glu  Gln Ala Glu Asn Ile  Ile His Leu 
    1520                 1525                 1530             


Phe Thr  Leu Thr Asn Leu Gly  Ala Pro Ala Ala Phe  Lys Tyr Phe 
    1535                 1540                 1545             


Asp Thr  Thr Ile Asp Arg Lys  Arg Tyr Thr Ser Thr  Lys Glu Val 
    1550                 1555                 1560             


Leu Asp  Ala Thr Leu Ile His  Gln Ser Ile Thr Gly  Leu Tyr Glu 
    1565                 1570                 1575             


Thr Arg  Ile Asp Leu Ser Gln  Leu Gly Gly Asp Ser  Gly Gly Ser 
    1580                 1585                 1590             


Lys Arg  Thr Ala Asp Gly Ser  Glu Phe Glu Pro Lys  Lys Lys Arg 
    1595                 1600                 1605             


Lys Val  
    1610 


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Test115F

<400>  26
tacaggcctc actggagcta                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Test115R

<400>  27
gaagcgacct ggacttttgc                                                   20


<210>  28
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Deepseq-HBG115-tryF

<400>  28
ccccttcccc acactatctc a                                                 21


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Deepseq-HBG115-tryR

<400>  29
attcttcatc cctagccagc c                                                 21


<210>  30
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  58-checksimilar-F

<400>  30
agcatcacaa caggcagaga at                                                22


<210>  31
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  58-checksimilar-R

<400>  31
gggaacacag atcctaacac agt                                               23


<210>  32
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DeepSPCR-F-try

<400>  32
gccagaaaag agatatggca tc                                                22


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DeepSPCR-R-try

<400>  33
agagagcctt ccgaaagagg                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nontrans_PCR-F5

<400>  34
gggaactagg aggccaaagt                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nontrans_PCR-R2

<400>  35
tgggcatgag attggggcaa                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nontrans_PCR-F3

<400>  36
gtccgcccca aggtcaaaaa                                                   20


<210>  37
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nontrans_PCR-R5

<400>  37
aggcatctgg caataatgag gt                                                22


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TFR2nontrans-DSF1

<400>  38
atcgctgggg gacagcctgc                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TFR2nontrans-DSR2

<400>  39
ctcaggggct tgggaggggg                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBG_-F

<400>  40
tgggtcattt cacagaggag                                                   20


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBG_-R

<400>  41
catcttccac attcaccttg c                                                 21


<210>  42
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBB_-F

<400>  42
cagtgcaggc tgcctatc                                                     18


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBB_-R

<400>  43
atacttgtgg gccagggcat                                                   20


<210>  44
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBA_-F

<400>  44
gccctggaga ggatgttc                                                     18


<210>  45
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBA_-R

<400>  45
ttcttgccgt ggccctta                                                     18


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DHS+55-F2

<400>  46
ttcattggcc gaggatgacc                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DHS+55-R2

<400>  47
acctcactgg tttctcccct                                                   20


