<110>    SAMYANG CORPORATION

<120>    Recombinant microorganism expressing fucosyltransferase and
         Method of producing 2'-fucolsylactose using thereof

<130>    OPP20215784KR

<150>    KR 10-2020-0189490
<151>    2020-12-31

<160>    63

<170>    koPatentIn 3.0

<210>    1
<211>    261
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of Am2FT_2


<400>    1
Met Asn Met Glu Arg Lys Thr Gly Leu Met Asn Lys Lys Tyr Val Ser
  1               5                  10                  15 

Pro Cys Phe Leu Pro Gly Met Arg Leu Gly Asn Ile Met Phe Thr Leu
             20                  25                  30 

Ala Ala Ala Cys Ala His Ala Arg Thr Val Gly Val Glu Cys Arg Val
         35                  40                  45 

Pro Trp Ala Tyr Asn Asp Ala Ser Leu Met Leu Arg Ser Arg Leu Gly
     50                  55                  60 

Gly Trp Val Leu Pro Ser Thr Pro Cys Gly Thr Asn Glu Pro Pro Ser
 65                  70                  75                  80 

Trp Gln Glu Pro Ser Phe Ala Tyr Cys Pro Val Pro Ser Arg Ile Arg
                 85                  90                  95 

Thr Gly Gly Leu Arg Gly Tyr Phe Gln Ser Ala Arg Tyr Phe Glu Gly
            100                 105                 110 

Gln Glu Ala Phe Ile Arg Ala Leu Phe Ala Pro Leu Thr Ala Glu Lys
        115                 120                 125 

Glu Pro Gly Ala Val Gly Ile His Ile Arg Leu Gly Asp Tyr Arg Arg
    130                 135                 140 

Leu Arg Asp Lys His Arg Ile Leu Asp Pro Gly Phe Leu Arg Arg Ala
145                 150                 155                 160 

Ala Gly His Leu Ser Ser Gly Lys Asn Arg Leu Val Leu Phe Ser Asp
                165                 170                 175 

Glu Pro Asp Glu Ala Ala Glu Met Leu Ala Arg Val Pro Ala Phe Gly
            180                 185                 190 

Arg Phe Ala Leu Glu Ile Asp Arg Gly Ala Pro Cys Glu Ser Leu Arg
        195                 200                 205 

Arg Met Thr Ala Met Glu Glu Leu Val Met Ser Cys Ser Ser Phe Ser
    210                 215                 220 

Trp Trp Gly Ala Trp Leu Gly Asn Thr Arg Lys Val Ile Val Pro Arg
225                 230                 235                 240 

Asp Trp Phe Val Gly Gly Val Glu Asp Tyr Arg Asp Ile Tyr Leu Pro
                245                 250                 255 

His Trp Val Thr Leu
            260    


<210>    2
<211>    291
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of Bm2FT


<400>    2
Met Lys Ile Val Gln Ile Ser Ser Gly Leu Gly Asn Gln Leu Phe Gln
  1               5                  10                  15 

Tyr Ala Leu Tyr Lys Arg Leu Ser Met Asn Asn Asn Asp Val Phe Leu
             20                  25                  30 

Asp Val Glu Thr Ser Tyr Gln Leu Asn Lys Asn Gln His Asn Gly Tyr
         35                  40                  45 

Glu Ile Glu Arg Ile Phe Ser Ile Gln Pro Ser His Ala Thr Lys Gly
     50                  55                  60 

Met Ile Asp Glu Leu Ala Asp Val Asp Asn Arg Leu Ile Asn Arg Leu
 65                  70                  75                  80 

Arg Arg Lys Leu Phe Gly Pro Lys Asn Ser Met Tyr Thr Glu Thr Lys
                 85                  90                  95 

Glu Phe Ser Tyr Asp Cys Glu Val Phe Thr Lys Asp Gly Ile Tyr Ile
            100                 105                 110 

Lys Gly Tyr Trp Gln Asn Tyr Asn Tyr Phe Lys Glu Ile Glu Asp Asp
        115                 120                 125 

Leu Lys Asn Glu Leu Val Phe Lys Lys Ala Leu Asp Leu Lys Asn Ser
    130                 135                 140 

His Leu Ile Asn Gln Met Asn Lys Glu Ile Ser Val Ser Ile His Val
145                 150                 155                 160 

Arg Arg Gly Asp Tyr Tyr Leu Asn Lys Glu Tyr Glu Asn Lys Phe Gly
                165                 170                 175 

Asn Ile Ala Asp Leu Asp Tyr Tyr Leu Lys Ala Ile Asn Phe Ile Lys
            180                 185                 190 

Lys Glu Val Asp Asp Pro Lys Phe Tyr Val Phe Ser Asp Asp Ile Lys
        195                 200                 205 

Trp Ala Lys Glu Asn Leu Asn Leu Thr Asp Asn Val Thr Tyr Val Glu
    210                 215                 220 

His Asn Lys Gly Ser Asp Ser Tyr Lys Asp Met Arg Leu Met Thr Cys
225                 230                 235                 240 

Cys Lys His Asn Ile Ile Ala Asn Ser Thr Phe Ser Trp Trp Gly Ala
                245                 250                 255 

Phe Leu Asn Glu Asn Lys Asn Lys Ile Val Ile Ala Pro Gly Lys Trp
            260                 265                 270 

Ile Asn Val Glu Gly Val Gly Gly Ile Asn Leu Phe Pro Glu Gly Trp
        275                 280                 285 

Ile Val Tyr
    290    


<210>    3
<211>    299
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of Bm2FT_2


<400>    3
Met Gly Cys Ile Lys Arg Leu Phe Leu Tyr Glu Tyr Gly Gly Arg Cys
  1               5                  10                  15 

Phe Leu Val Ile Val Lys Ile Lys Gly Gly Phe Gly Asn Gln Leu Phe
             20                  25                  30 

Thr Tyr Ala Ser Ala Tyr Ala Ile Ser Gln Glu Leu Gln Gln Asn Leu
         35                  40                  45 

Ile Met Asp Lys Val Ile Tyr Asp Leu Asp Tyr Phe Arg Lys Phe Glu
     50                  55                  60 

Leu Pro Ser Leu Ser Leu Lys Tyr Asp Gln Met Leu Ile Ser Lys Phe
 65                  70                  75                  80 

Val Pro Asn Thr Lys Val Lys Thr Leu Ile Tyr Lys Val Leu Arg Arg
                 85                  90                  95 

Leu Lys Leu Lys Gly Phe Thr Glu Val His Glu Lys Lys Glu Phe Ser
            100                 105                 110 

Phe Asp Glu Asn Ile Tyr Asn Leu Ser Gly Asp Ile Tyr Leu Asp Gly
        115                 120                 125 

Tyr Trp Gln Asn Tyr Arg Tyr Phe His Lys Tyr Tyr Lys Asp Leu Ser
    130                 135                 140 

Glu Met Phe Val Pro Arg Glu Thr Pro Arg Lys Glu Val Thr Asp Tyr
145                 150                 155                 160 

Ile Thr Ser Leu Arg Gly Val Asn Ser Val Ala Met His Val Arg Arg
                165                 170                 175 

Gly Asp Tyr Lys Thr Phe Asn Gly Gly Lys Cys Leu Ser Leu Asp Tyr
            180                 185                 190 

Tyr Ile Lys Ala Met Glu Tyr Phe Asn Ser Asn Asp Val Gln Phe Tyr
        195                 200                 205 

Val Phe Thr Asp Asp Ile Asp Phe Cys Glu Lys Asn Leu Pro Asn Ser
    210                 215                 220 

Glu Asn Ile Asn Tyr Val Ser Arg Ser Glu Lys Leu Thr Asp Ile Glu
225                 230                 235                 240 

Glu Phe Phe Ile Met Lys Glu Cys Lys Asn Phe Ile Ile Ala Asn Ser
                245                 250                 255 

Ser Phe Ser Trp Trp Ala Ala Tyr Leu Ser Glu Gln Lys Ala Asp Ser
            260                 265                 270 

Leu Ile Val Ala Pro Val Val Asp Met Trp Lys Arg Asp Phe Tyr Pro
        275                 280                 285 

Asp Glu Trp Val Ala Leu Asn Thr His Leu Glu
    290                 295                


<210>    4
<211>    287
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of Bm2FT_3


<400>    4
Met Ile Ile Val Gln Ile Lys Gly Gly Leu Gly Asn Gln Leu Phe Ser
  1               5                  10                  15 

Tyr Ala Ser Ala Tyr Gly Ile Ala Arg Glu Asn Glu Thr Glu Leu Ile
             20                  25                  30 

Ile Asp Arg Tyr Ile Tyr Asp Thr Ser Tyr Ser Leu Arg Lys Tyr Met
         35                  40                  45 

Leu Asp Phe Phe Pro Glu Ile Asp Glu Ala Leu Leu Leu Lys Tyr Ile
     50                  55                  60 

Pro Lys Lys Asn Lys Ile Ser Gln Ile Met Tyr Lys Leu Ile Arg Lys
 65                  70                  75                  80 

Ser Lys Leu Lys Tyr Lys Tyr Lys Ala Gln Leu Phe Leu Glu Glu Glu
                 85                  90                  95 

Glu Phe Lys Phe Thr Arg Ile Ser Thr Gln Asn Glu Asn Leu Tyr Leu
            100                 105                 110 

Asn Gly Tyr Trp Gln Ser Tyr Val Tyr Phe Asp Lys Tyr Arg Asn Asp
        115                 120                 125 

Ile Ile Lys Lys Phe Thr Pro Leu Val Ser Phe Asn Gln Asp Gly Asn
    130                 135                 140 

Gln Leu Leu Asn Glu Ile Lys Ser Tyr Asn Ser Val Ala Ile His Val
145                 150                 155                 160 

Arg Arg Gly Asp Tyr Ile Asn Phe Lys Gly Gly Lys Cys Leu Asp Ser
                165                 170                 175 

Ser Tyr Tyr Ile Lys Ala Met Lys His Leu Tyr Asn Leu Lys Gly Lys
            180                 185                 190 

Asn Leu Phe Phe Tyr Ile Phe Thr Asp Asp Val Glu Tyr Cys Lys Arg
        195                 200                 205 

Ile Phe Lys Asn Val Ala Asn Val Lys Phe Ile Gly Glu Glu Ala Lys
    210                 215                 220 

Leu Ser Asp Phe Glu Glu Phe Thr Leu Met Thr His Cys Lys Asn Leu
225                 230                 235                 240 

Ile Ile Gly Asn Ser Ser Phe Ser Trp Trp Ala Ala Tyr Leu Ala Ser
                245                 250                 255 

Cys Lys Asp Lys Ala Val Ile Ala Pro Val Val Asp Met Trp Thr Glu
            260                 265                 270 

Asp Phe Tyr Leu Pro Glu Trp Ile Lys Ile Lys Ala Asp Leu Gln
        275                 280                 285 




<210>    5
<211>    291
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of Bs2FT


<400>    5
Met Lys Ile Val His Ile Ser Ser Gly Leu Gly Asn Gln Met Phe Gln
  1               5                  10                  15 

Tyr Ala Leu Tyr Lys Lys Leu Ser Leu Ile Gln Asp Asn Val Phe Leu
             20                  25                  30 

Asp Thr Ile Thr Ser Tyr Gln Leu Tyr Pro Asn Gln His Asn Gly Tyr
         35                  40                  45 

Glu Leu Glu Lys Val Phe Thr Ile Lys Pro Arg His Ala Ser Lys Glu
     50                  55                  60 

Leu Thr Tyr Asn Leu Ser Asp Leu Asp Asn Ser Val Thr Ser Arg Ile
 65                  70                  75                  80 

Arg Arg Lys Leu Ile Gly Ser Lys Lys Ser Met Tyr Ile Glu His Lys
                 85                  90                  95 

Glu Phe Glu Tyr Asp Pro Asn Leu Phe Tyr Gln Glu Asn Ile Tyr Ile
            100                 105                 110 

Lys Gly Tyr Trp Gln Asn Tyr Asp Tyr Phe Lys Asp Ile Glu Asn Glu
        115                 120                 125 

Leu Lys Asn Asp Phe Thr Phe Gln Arg Ala Leu Asp Glu Lys Asn Asn
    130                 135                 140 

Asn Leu Ala Ile Lys Ile Asn Asn Glu Asn Ser Ile Ser Ile His Val
145                 150                 155                 160 

Arg Arg Gly Asp Tyr Tyr Leu Asn Arg Lys Asn Gln Glu Lys Phe Gly
                165                 170                 175 

Asp Ile Ala Asn Leu Glu Tyr Tyr Ser Lys Ala Ile Ser Tyr Ile Lys
            180                 185                 190 

Glu Arg Ile Asp Asn Pro Lys Phe Tyr Ile Phe Ser Asp Asn Val Glu
        195                 200                 205 

Trp Val Lys Gln Asn Leu Asn Ser Leu Glu Glu Ala Val Tyr Ile Asp
    210                 215                 220 

Tyr Asn Val Gly Asn Asp Ser Tyr Lys Asp Met Gln Leu Met Ser Leu
225                 230                 235                 240 

Cys Lys His Asn Ile Ile Ala Asn Ser Ser Phe Ser Trp Trp Gly Ala
                245                 250                 255 

Phe Leu Asn Lys Asn Met Glu Lys Ile Val Ile Ala Pro Gly Lys Trp
            260                 265                 270 

Ile Asn Met Lys Gly Val Lys Lys Val Asn Leu Phe Pro Lys Asp Trp
        275                 280                 285 

Ile Ile Tyr
    290    


<210>    6
<211>    289
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of Bm2FT_4


<400>    6
Met Ile Ile Val Arg Val Ile Gly Gly Leu Gly Asn Gln Met Phe Gln
  1               5                  10                  15 

Tyr Ala Leu Tyr Lys Ser Leu Glu Asn Glu Gly Lys Glu Val Lys Leu
             20                  25                  30 

Asp Leu Thr Gly Phe Gly Asp Tyr Asp Leu His Asn Gly Tyr Glu Leu
         35                  40                  45 

Asn Lys Ile Phe Asn Ile Asn Glu Asn Val Ala Thr Lys Asp Glu Ile
     50                  55                  60 

Asn Lys Leu Ile Lys Leu Pro Asp Asn Lys Val Leu Ser Met Ile Lys
 65                  70                  75                  80 

Arg Lys Phe Phe Ser Ser Thr Ile Asn Tyr Tyr Ser Gln Asp Gln Phe
                 85                  90                  95 

Lys Tyr Leu Ser Glu Ile Phe Gln Leu Asp Asn Val Tyr Leu Asp Gly
            100                 105                 110 

Tyr Trp Gln Ser Glu Lys Tyr Phe Gln Gly Ile Lys Glu Val Ile Arg
        115                 120                 125 

Lys Glu Phe Lys Phe Lys Gly Glu Pro Asn Pro Lys Asn Ile Glu Met
    130                 135                 140 

Val Lys Leu Met Arg Gly Ser Asn Ser Val Ser Ile His Phe Arg Arg
145                 150                 155                 160 

Gly Asp Tyr Ile Ser Asn Pro Asp Ala Tyr Lys Val His Gly Gly Ile
                165                 170                 175 

Thr Thr Ile His Tyr Tyr Glu Asn Ala Val Lys Glu Ile Lys Ser Lys
            180                 185                 190 

Val Lys Glu Pro Lys Phe Phe Ile Phe Ser Asp Asp Ile Lys Trp Val
        195                 200                 205 

Lys Glu Asn Phe Lys Leu Glu Asp Ala Phe Phe Ile Asp Trp Asn Thr
    210                 215                 220 

Gly Ser Glu Ser Tyr Arg Asp Ile Glu Leu Met Ser Asn Cys Lys His
225                 230                 235                 240 

Asn Ile Ile Ala Asn Ser Thr Phe Ser Trp Trp Gly Ala Trp Leu Asn
                245                 250                 255 

Lys Asn Lys Asn Lys Ile Val Ile Ala Pro Asn Gln Trp Phe Asn Thr
            260                 265                 270 

Ile Asp Thr Glu Asp Val Ile Pro Asp Thr Trp Gln Cys Ile Asn Thr
        275                 280                 285 

Phe
   


<210>    7
<211>    308
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of Bt2FT


<400>    7
Met Asn Glu Ile His Val Leu Leu Thr Gly Arg Leu Gly Asn Gln Leu
  1               5                  10                  15 

Phe Gln Tyr Ala Phe Ala Arg Ser Leu Gln Lys Gln Tyr Gly Gly Arg
             20                  25                  30 

Ile Val Cys Asn Thr Phe Asp Leu Asp His Arg Ser Glu Lys Ser Lys
         35                  40                  45 

Val Ala Asp Gln Lys Phe Ser Tyr Ala Met Gly Asp Phe Lys Leu Asp
     50                  55                  60 

Glu Asn Val Ala Leu Glu Asp Ala Ala Leu Pro Trp Tyr Ala Asp Phe
 65                  70                  75                  80 

Ser Ser Pro Leu Ile Lys Pro Val Lys Lys Leu Phe Pro Arg Arg Tyr
                 85                  90                  95 

Phe Asp Phe Met Ala Arg Arg Gly Tyr Leu Met Trp Gln Arg Thr Asp
            100                 105                 110 

Tyr Met Pro Ile Pro Lys Leu Glu Cys Glu Asp Val Phe Ala Trp Gly
        115                 120                 125 

Trp Trp Gln Asp Ile Arg Tyr Phe Gln Asp Val Gln Thr Glu Leu Ser
    130                 135                 140 

Asp Glu Val Val Pro Val Thr Asp Pro Leu Pro Glu Asn Gln Tyr Ile
145                 150                 155                 160 

Tyr Asn Ala Ala Ser Gly Glu Glu Ser Val Cys Ile Ser Ile Arg Cys
                165                 170                 175 

Gly Asn Tyr Phe Asn Pro Thr Val Lys Lys Leu Leu Tyr Val Cys Thr
            180                 185                 190 

Pro Glu Tyr Phe Arg Asn Ala Val Glu Ser Ile Thr Lys Arg Leu Thr
        195                 200                 205 

His Pro Lys Phe Ile Val Phe Thr Asp Asp Val Asp Trp Val Lys Glu
    210                 215                 220 

Asn Ile Arg Phe Glu Ser Ala Tyr Pro Gln Tyr Glu Phe Leu Tyr Glu
225                 230                 235                 240 

Arg Gly Ser Asp Thr Val Glu Glu Lys Ile Arg Met Met Thr Leu Cys
                245                 250                 255 

Lys His Phe Ile Ile Ser Asn Ser Thr Phe Ser Trp Trp Ala Gln Phe
            260                 265                 270 

Leu Ser Lys Ser Glu Asn Lys Ile Val Ile Ala Pro Asp Arg Trp Phe
        275                 280                 285 

Val Asp Gly Arg Arg Ile Gly Leu Tyr Met His Gly Trp Thr Leu Ile
    290                 295                 300 

Pro Ala Gly Gln
305            


<210>    8
<211>    317
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of Fp2FT


<400>    8
Met Ile Tyr Val Glu Met His Gly Arg Leu Gly Asn Gln Met Phe Gln
  1               5                  10                  15 

Tyr Ala Ala Ala Arg Ala Leu Gln Glu Lys Asn Asn Gln Pro Ile Met
             20                  25                  30 

Leu Ser Phe Arg Lys Val Ile Gly Ala Asn Thr Glu Gly Thr Ala Gly
         35                  40                  45 

Trp Glu Asn Ser Leu Lys Tyr Phe Asn Val Lys Pro Cys Glu Tyr Tyr
     50                  55                  60 

Met Gly Lys Lys Ser Leu Val Thr Glu Tyr Pro Val Glu Tyr Arg Leu
 65                  70                  75                  80 

Leu Cys Tyr Ala Tyr Ala Leu Ser Tyr Lys Pro Leu Met Asn Asn Met
                 85                  90                  95 

Asn Arg Trp Tyr Glu Tyr Gln Val Lys Cys Cys Arg Phe Leu Asp Arg
            100                 105                 110 

Phe Gly Ile Arg Trp Ile Ala Asn Gly Tyr Tyr Asp Phe His Tyr Asn
        115                 120                 125 

Gly Leu Lys Asn Tyr Leu Leu Asn Gly Ser Phe Glu Ser Pro Lys Tyr
    130                 135                 140 

Phe Asp Ser Ile Arg Asp Lys Leu Leu Glu Glu Phe Thr Pro Arg Glu
145                 150                 155                 160 

Glu Glu Arg Lys Glu Asn Lys Arg Leu Tyr Glu Gln Ile Arg Lys Arg
                165                 170                 175 

Asn Ser Val Cys Leu Ser Val Arg His Phe Gln Leu Thr Gly Lys Gln
            180                 185                 190 

Ala Asp Met Tyr Asp Val Cys Ser Leu Glu Tyr Tyr Gln Thr Ala Ile
        195                 200                 205 

Arg Lys Met Cys Glu Leu Ile Glu Asn Pro Leu Phe Val Val Phe Ser
    210                 215                 220 

Asp Asp Ile Glu Trp Val Lys Asn Thr Ile Asp Leu Ser Arg Val Glu
225                 230                 235                 240 

Val Val Tyr Glu Thr Pro Gly Asn Pro Val Trp Glu Lys Leu Arg Leu
                245                 250                 255 

Met Tyr Ser Cys Lys Asn Phe Ile Ile Pro Asn Ser Thr Phe Ala Trp
            260                 265                 270 

Trp Ala Gln Tyr Leu Ser Arg Asn Pro Asp Lys Tyr Val Leu Cys Pro
        275                 280                 285 

Ala Lys Trp Phe Asn Asn Asn Phe Glu Ser Pro Leu Ile Ala Ser Gln
    290                 295                 300 

Trp Val Arg Ile Asp Arg Glu Gly Asn Ile Val Asn Glu
305                 310                 315        


<210>    9
<211>    289
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of Gs2FT


<400>    9
Met Lys Ile Val Lys Val Ile Gly Gly Leu Gly Asn Gln Met Phe Gln
  1               5                  10                  15 

Tyr Ala Phe Tyr Arg Asn Leu Lys Ala Lys Phe Gln Glu Val Lys Leu
             20                  25                  30 

Asp Ile Thr Ala Phe Glu Thr Tyr Lys Leu His Asn Gly Tyr Glu Leu
         35                  40                  45 

Glu Arg Val Phe Asp Ile Lys Pro Glu Tyr Ala Thr Lys Lys Glu Ile
     50                  55                  60 

Tyr Pro Leu Thr Thr Asn Arg Asn Ser Lys Ile Ser Lys Ile Lys Arg
 65                  70                  75                  80 

Arg Ile Phe Gly Gly Lys Glu Thr Glu Tyr Ile Glu Lys Asp Leu Lys
                 85                  90                  95 

Phe Asp Pro Glu Val Phe Lys Val Thr Gly Asp Val Tyr Phe Glu Gly
            100                 105                 110 

Tyr Trp Gln Thr Glu Lys Tyr Phe Lys Glu Ile Glu Asp Leu Ile Arg
        115                 120                 125 

Lys Asp Phe Gln Phe Lys Asn Pro Leu Thr Asn Lys Asn Leu Glu Leu
    130                 135                 140 

Ser Asn Lys Ile Lys Asn Glu Asn Ser Val Ser Ile His Val Arg Arg
145                 150                 155                 160 

Gly Asp Tyr Tyr Thr Ser Lys Lys Ala Glu Arg Lys His Gly Asn Ile
                165                 170                 175 

Ala Thr Ile Glu Tyr Tyr Gln Lys Ala Val Arg Lys Ile Thr Glu Phe
            180                 185                 190 

Val Asp Asn Pro Val Phe Tyr Ile Phe Ser Asp Asp Ile Pro Trp Val
        195                 200                 205 

Lys Glu Asn Leu Lys Leu Glu Asn Glu Val Ile Tyr Val Asp Trp Asn
    210                 215                 220 

Lys Gly Leu Asp Ser Tyr Ile Asp Met Gln Leu Met Ser Ile Cys Lys
225                 230                 235                 240 

His Asn Ile Ile Ala Asn Ser Thr Phe Ser Trp Trp Gly Ala Trp Leu
                245                 250                 255 

Asn Gln Asn Lys Asn Lys Ile Val Ile Ala Pro Ser Arg Trp Ile Asn
            260                 265                 270 

Asn Lys Arg Leu Asp Thr Ser Asp Val Ile Pro Lys Glu Trp Ile Lys
        275                 280                 285 

Ile
   


<210>    10
<211>    314
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of Lg2FT


<400>    10
Met Leu Tyr Val Glu Met Asp Gly Arg Cys Gly Asn Gln Leu Phe His
  1               5                  10                  15 

Tyr Ala Val Ala Arg Tyr Ile Gln Leu Ala Ile Gly Asn Lys Glu Lys
             20                  25                  30 

Leu Cys Leu Asn Phe Asn Lys Ile Phe Glu Lys Lys Asp Glu Asn Asn
         35                  40                  45 

Gly Trp Val Asp Tyr Leu Lys Asp Phe Lys Thr Val Pro Tyr Ser Tyr
     50                  55                  60 

Tyr Ser Lys Ser Gly Thr Ile Leu Lys Asn Glu Ser Asn Phe Ile Gln
 65                  70                  75                  80 

Lys Ile Ala Ile Gly Leu Lys Ala Ile Gln Ile Lys Ser Leu Thr Lys
                 85                  90                  95 

Lys Ser Arg Gln Glu Gln Ala Asp Lys Ala Glu Val Gly Gln Arg Thr
            100                 105                 110 

Leu Asn Lys Leu Gly Val Tyr Trp Val Arg Glu Gly Val Asn Gln Ile
        115                 120                 125 

Tyr Pro Tyr Lys Asn Asn Lys Ile Leu Val Ser Gly Ile Cys Glu Ser
    130                 135                 140 

Asn Phe Ile Tyr Glu Ile Gln Glu Gln Leu Gln Lys Glu Leu Ile Pro
145                 150                 155                 160 

Val Thr Pro Val Ser Ser Leu Asn Lys Ser Leu Leu Glu Lys Ile Asp
                165                 170                 175 

Asn Cys Asn Ser Val Cys Ile Ser Val Arg Arg Gly Asp Phe Phe Asn
            180                 185                 190 

Asn Lys Asn Ala Lys Lys Tyr Gly Val Cys Ser Pro Glu Tyr Tyr Ile
        195                 200                 205 

Arg Ala Lys Lys Tyr Phe Asp Lys Lys Arg Leu Glu Asn Thr Val Tyr
    210                 215                 220 

Phe Cys Phe Ser Asp Asp Ile Glu Trp Cys Lys Glu Asn Leu Lys Phe
225                 230                 235                 240 

Thr Asp Lys Asn Val Ile Phe Val Ser Gln Glu Met Pro Val Tyr Glu
                245                 250                 255 

Thr Leu Arg Leu Met Ser His Cys Lys His Phe Ile Leu Ser Asn Ser
            260                 265                 270 

Thr Phe Ser Trp Trp Gly Gln Phe Leu Ser Glu Tyr Lys Asp Lys Ile
        275                 280                 285 

Val Val Ser Pro Ala Arg Trp Asn Asn Asp Gly Tyr Asp Thr Asn Leu
    290                 295                 300 

Ile Asp Lys Asn Trp Ile Leu Ile Asp Ala
305                 310                


<210>    11
<211>    309
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of Ll2FT


<400>    11
Met Ile Tyr Val Glu Ile Arg Gly Asn Leu Gly Asn Gln Leu Phe Ile
  1               5                  10                  15 

Tyr Ala Thr Ala Lys Lys Ile Gln Lys Leu Thr Gly Gln Lys Ile Gln
             20                  25                  30 

Leu Asn Thr Thr Thr Leu Asn Lys Tyr Phe Pro Asn Tyr Lys Phe Gly
         35                  40                  45 

Leu Ser Glu Phe Ile Met Glu Asp Pro Asp Cys Phe Ile Glu Ser Tyr
     50                  55                  60 

Lys Lys Leu Pro Trp Phe Thr Asn Glu Tyr Leu Leu Pro Ile Lys Ile
 65                  70                  75                  80 

Phe Lys Lys Ile Leu Asn Lys Thr Pro Lys Ile Asn Lys Ile Leu Ser
                 85                  90                  95 

Asp Phe Phe Phe Lys Ala Phe Glu Lys Lys Gly Tyr Phe Ile Trp Arg
            100                 105                 110 

Gly Glu Thr Phe Lys Lys Phe Ser Leu Gly Asn His Lys Asn Tyr Tyr
        115                 120                 125 

Leu Ser Gly Phe Trp Gln Ser Glu Asp Tyr Phe Tyr Asp Ile Arg Asp
    130                 135                 140 

Glu Leu Leu Glu Ile Ile Thr Pro Ile Asn Ser Ile Arg Glu Cys Asn
145                 150                 155                 160 

Phe Glu Leu Leu Asn Leu Ile Arg Asn Ser Glu Ser Ile Cys Val Ser
                165                 170                 175 

Ile Arg Arg Gly Asp Tyr Val Asp Asn Pro Lys Ile Ser Ala Ile Tyr
            180                 185                 190 

Asn Val Cys Asp Ile Asn Tyr Phe Ile Glu Ser Val Asn Glu Ile Lys
        195                 200                 205 

Lys Asn Val Val Asn Val Lys Val Ile Cys Phe Ser Asp Asp Val Glu
    210                 215                 220 

Trp Val Lys Lys Asn Ile Lys Phe Asp Cys Glu Thr His Tyr Glu Thr
225                 230                 235                 240 

Tyr Gly Asn Ser Leu Ser Glu Lys Val Gln Leu Met Ser Ser Cys Lys
                245                 250                 255 

His Phe Val Leu Ser Asn Ser Ser Phe Ser Trp Trp Thr Glu Phe Leu
            260                 265                 270 

Ser Ile Arg Gly Gly Ile Thr Ile Ala Pro Lys Asn Trp Tyr Ala Asp
        275                 280                 285 

Glu Arg Glu Ala Asp Ile Tyr Arg Lys Asn Trp Ile Tyr Leu Glu Asp
    290                 295                 300 

Lys Thr Glu Glu Glu
305                


<210>    12
<211>    306
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of Ls2FT


<400>    12
Met Glu Glu Ile His Thr Leu Leu Thr Gly Arg Leu Gly Asn Gln Leu
  1               5                  10                  15 

Phe Gln Tyr Ala Phe Ala Arg Asn Leu Gln Lys Gln Tyr Gly Gly Gln
             20                  25                  30 

Ile Tyr Cys Asp Val Tyr Glu Leu Glu His Arg Met Ser Lys Val Ala
         35                  40                  45 

Asp Glu Lys Phe Ser Tyr Ala Met Ser Gly Phe Lys Leu Asp Ala Gly
     50                  55                  60 

Val Ile Arg Glu Asp Gln Ala Phe Pro Trp Tyr Ala Asp Phe Ser Asn
 65                  70                  75                  80 

Pro Val Ile Lys Pro Ile Lys Lys Ala Met Pro Arg Lys Tyr Phe Glu
                 85                  90                  95 

Leu Met Ala Arg Arg Gly Tyr Leu Met Trp Gln Arg Ser Asp Tyr Met
            100                 105                 110 

Pro Ile Pro Val Leu Asp Thr Gln Arg Val Phe Ala Ser Gly Trp Trp
        115                 120                 125 

Gln Asp Ile Arg Tyr Leu Gln Asn Val Gln Glu Glu Leu Ser Asp Glu
    130                 135                 140 

Ile Val Pro Ile Thr Asn Pro Leu Gln Glu Asn Arg Tyr Ile Tyr Asp
145                 150                 155                 160 

Ala Ala His Asp Arg Asp Ser Val Cys Ile Ser Ile Arg Cys Gly Asn
                165                 170                 175 

Tyr Phe Asn Pro Thr Val Lys Lys Leu Leu Tyr Val Cys Asn Pro Gln
            180                 185                 190 

Tyr Phe His Asp Ser Val Lys Arg Ile Ser Gln Met Leu Ala His Pro
        195                 200                 205 

Lys Phe Ile Val Phe Thr Asp Asp Val Ser Trp Val Lys Glu His Leu
    210                 215                 220 

Lys Phe Glu Asp Thr Tyr Pro Gln Phe Glu Phe Leu Tyr Glu Arg Gly
225                 230                 235                 240 

Cys Asp Thr Ala Glu Glu Lys Ile Arg Met Met Ala Met Cys Asn Asn
                245                 250                 255 

Phe Ile Ile Ser Asn Ser Thr Phe Ser Trp Trp Ala Gln Phe Leu Ser
            260                 265                 270 

Lys Asn Lys Glu Lys Ile Val Ile Ala Pro Asp Lys Trp Phe Val Asp
        275                 280                 285 

Gly Arg Lys Ile Gly Leu Tyr Met Asp Gly Trp Thr Leu Val Pro Ala
    290                 295                 300 

Gly Arg
305    


<210>    13
<211>    292
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of Ri2FT


<400>    13
Met Ile Ala Val Lys Ile Gly Asp Gly Met Gly Asn Gln Leu Phe Asn
  1               5                  10                  15 

Tyr Ala Cys Gly Tyr Ala Gln Ala Arg Arg Asp Gly Asp Ser Leu Val
             20                  25                  30 

Leu Asp Ile Ser Glu Cys Asp Asn Ser Thr Leu Arg Asp Phe Glu Leu
         35                  40                  45 

Asp Lys Phe His Leu Lys Tyr Asp Lys Lys Glu Ser Phe Pro Asn Arg
     50                  55                  60 

Asn Leu Gly Gln Lys Ile Tyr Lys Asn Leu Arg Arg Ala Leu Lys Tyr
 65                  70                  75                  80 

His Val Ile Lys Glu Arg Glu Val Tyr His Asn Arg Asp His Arg Tyr
                 85                  90                  95 

Asp Val Asn Asp Ile Asp Pro Arg Val Tyr Lys Lys Lys Gly Leu Arg
            100                 105                 110 

Asn Lys Tyr Leu Tyr Gly Tyr Trp Gln His Leu Ala Tyr Phe Glu Asp
        115                 120                 125 

Tyr Leu Asn Glu Ile Thr Ala Met Met Thr Pro Ala Tyr Glu Gln Ser
    130                 135                 140 

Glu Thr Val Lys Lys Leu Gln Glu Glu Phe Lys Lys Thr Pro Thr Cys
145                 150                 155                 160 

Ala Val His Val Arg Gly Gly Asp Ile Met Gly Pro Ala Gly Ala Tyr
                165                 170                 175 

Phe Lys His Ala Met Glu Arg Met Glu Gln Glu Lys Pro Gly Val Arg
            180                 185                 190 

Tyr Ile Val Phe Thr Asn Asp Met Glu Arg Ala Glu Glu Ala Leu Ala
        195                 200                 205 

Pro Val Leu Glu Ser Gln Lys Lys Asp Ala Val Gly Gln Ala Glu Asn
    210                 215                 220 

Arg Leu Glu Phe Val Ser Glu Met Gly Glu Phe Ser Asp Val Asp Glu
225                 230                 235                 240 

Phe Phe Leu Met Ala Ala Cys Gln Asn Gln Ile Leu Ser Asn Ser Thr
                245                 250                 255 

Phe Ser Thr Trp Ala Ala Tyr Leu Asn Gln Asn Pro Asp Lys Thr Val
            260                 265                 270 

Ile Met Pro Asp Asp Leu Leu Ser Glu Arg Met Arg Gln Lys Asn Trp
        275                 280                 285 

Ile Ile Leu Lys
    290        


<210>    14
<211>    786
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleotide sequence encoding Am2FT_2


<400>    14
atgaacatgg aacggaaaac cggccttatg aataaaaaat atgtttcccc gtgtttcctg         60

cccggcatgc ggctgggcaa catcatgttc acgctggcgg ccgcctgtgc ccacgcccgt        120

accgtagggg tggagtgccg ggtgccctgg gcttacaatg acgcttccct gatgctgcgc        180

tcccggctcg gcggctgggt gctcccttct acgccgtgcg gcaccaatga acctccatcc        240

tggcaggaac cttcctttgc ctattgtccg gttccctccc gcatccggac gggcgggctt        300

cgcggctact tccagagcgc acggtatttt gaagggcagg aagcgttcat ccgggcgctt        360

ttcgcgcctc tcaccgcgga gaaggagccc ggcgccgttg gcattcacat ccggcttggg        420

gactacaggc gcctccgtga caagcaccgc attctggacc ctggtttcct gcggagggcc        480

gcagggcatt tatcatccgg gaagaaccgt ctggtgctgt tcagcgatga gccggacgag        540

gcggcggaga tgcttgcgcg cgtgccggca ttcggacgct ttgcgctgga aatcgaccgc        600

ggcgctccgt gcgaatccct ccgccgcatg acggcgatgg aggaactcgt catgtcgtgt        660

tcttcctttt cctggtgggg cgcgtggctg ggaaataccc ggaaggtcat cgttccgcgc        720

gactggtttg tcggcggtgt ggaagactac cgggatattt acctgcccca ttgggtaaca        780

ctgtaa                                                                   786


<210>    15
<211>    876
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleotide sequence encoding Bm2FT


<400>    15
atgaaaattg ttcagatttc ttctggtctg ggtaaccagc tgttccagta cgccctgtac         60

aaacgtctgt ctatgaacaa caacgatgtg ttcctggatg tggagacgtc ttatcaactg        120

aacaaaaatc agcacaatgg ttacgaaatt gaacgtattt tttccatcca gccatcccat        180

gctaccaagg gtatgatcga tgaactggcc gatgtagaca accgtctgat caaccgctta        240

cgtcgtaaac tgtttggtcc gaaaaactct atgtataccg aaaccaaaga gttcagctac        300

gattgtgaag ttttcaccaa agatggtatc tatatcaagg gctactggca gaactataac        360

tattttaaag agattgaaga cgatctgaaa aatgaactgg tctttaaaaa agctctggat        420

ctgaaaaatt cgcacctgat caatcagatg aataaagaaa ttagcgtaag catccacgta        480

cgccgcggcg attattacct gaacaaggag tacgaaaaca aatttggcaa cattgcggat        540

cttgattatt atctgaaagc gattaacttc atcaaaaaag aagtagacga tcccaaattt        600

tacgttttct ccgacgatat taaatgggct aaagaaaatc tgaacctgac ggataacgta        660

acttatgttg aacacaacaa agggagcgac tcttacaaag acatgcgtct gatgacctgc        720

tgtaaacata acatcatcgc caactctaca ttctcttggt ggggcgcgtt tcttaacgag        780

aacaagaaca aaatcgtaat cgcgccgggc aaatggatca acgttgaagg cgtcggtggc        840

atcaacctgt tccctgaagg ttggatcgtt tactga                                  876


<210>    16
<211>    900
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleotide sequence encoding Bm2FT_2


<400>    16
atgggttgca taaaaagact attcttatat gaatacggag gacgatgttt tttggtaatt         60

gttaaaataa aaggtggatt tggtaaccaa ttgtttacat acgctagtgc ttatgcaata        120

tctcaagaac tgcaacaaaa cttaattatg gataaggtaa tatatgatct agattacttt        180

agaaaatttg aattaccttc attatcgttg aagtatgatc aaatgttaat tagtaagttt        240

gtacctaata ctaaggttaa aactttaatt tataaagttt taaggaggtt aaaattaaaa        300

ggatttacag aggtacatga aaaaaaggaa ttttcttttg atgaaaatat ttacaatttg        360

tcaggtgata tttatttaga tggttattgg cagaattata gatatttcca taaatattat        420

aaagatctgt cggaaatgtt tgtaccaaga gaaactccta gaaaagaagt aactgattat        480

atcacgtctt tacggggagt taattctgtt gctatgcatg ttaggagagg agattataaa        540

acttttaacg gaggaaaatg tttgtcttta gattattata ttaaggctat ggaatatttc        600

aattcaaatg atgtacaatt ttatgtattt acagatgata ttgatttttg tgagaaaaac        660

ctcccgaata gtgaaaatat taattatgtg tcaagaagcg agaaacttac agatattgaa        720

gaattcttta ttatgaaaga atgtaaaaat tttataattg ctaatagtag tttttcgtgg        780

tgggctgctt atttaagtga acagaaagca gatagtttaa ttgtagcccc tgttgttgac        840

atgtggaaaa gagattttta tccagatgag tgggtggcat tgaatacaca tttagaatag        900

                                                                         900


<210>    17
<211>    864
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleotide sequence encoding Bm2FT_3


<400>    17
atgataattg ttcaaatcaa gggcggatta ggtaatcaac ttttttcgta tgcttctgct         60

tatggaattg caagagagaa tgaaacagaa ttaattattg atcgatatat atatgataca        120

agctactctt taagaaaata tatgttggat tttttccctg agatcgatga agcactactt        180

ctaaagtata tacctaaaaa aaataaaatt tcacaaatta tgtataaatt gattagaaaa        240

tctaagttga aatataagta caaagctcaa ttatttttag aagaagagga atttaagttt        300

actagaatta gtacccagaa tgaaaatttg tatttaaatg gttattggca aagttatgtg        360

tactttgata aatatcgtaa tgacattatt aaaaaattca cccctttagt aagctttaat        420

caagatggaa atcaacttct taacgaaatt aaaagttata attcagtggc tatccatgtc        480

aggagagggg attatattaa ttttaaaggt ggaaagtgtt tagatagttc ttattatata        540

aaggcaatga agcatttata caatttaaaa ggaaaaaatt tgttttttta tatttttaca        600

gatgatgtgg aatattgtaa aagaattttt aaaaacgtag ctaatgttaa atttataggt        660

gaggaagcaa aattgagtga ttttgaagag tttactttga tgactcattg taaaaacctc        720

ataataggta acagctcatt ttcttggtgg gcagcttact tagcctcttg taaagataaa        780

gctgtaatag ctccagtggt agatatgtgg actgaagatt tctatctacc tgaatggata        840

aaaattaaag cggatttaca atag                                               864


<210>    18
<211>    876
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleotide sequence encoding Bs2FT


<400>    18
atgaaaatag tacacataag tagcggttta ggaaatcaaa tgtttcaata tgctctatat         60

aaaaaactgt ccttaattca agataatgtt tttttggata caattaccag ttatcagttg        120

tatcctaatc aacataatgg atatgagtta gagaaggtat ttacaattaa accaaggcat        180

gcaagtaaag aattaacata caatttatca gacttagata attcagtaac atctcgcatt        240

cgtaggaaat taattggaag taaaaagagc atgtatatag aacataaaga atttgaatac        300

gatcctaatt tattttatca agaaaatata tatataaaag gttactggca gaattatgat        360

tattttaaag atatagaaaa tgaattaaag aatgatttta cttttcaaag agctttagat        420

gagaaaaata ataatttagc tattaaaatt aacaatgaga attcaatatc aattcatgta        480

agacgtggtg attattactt aaacagaaag aatcaagaga agtttggaga tatcgctaat        540

ttggaatact attcaaaggc aattagctat attaaagaaa gaatagacaa tccaaagttt        600

tatatctttt cagataatgt tgagtgggta aagcaaaatc ttaattcgtt agaggaagct        660

gtatatatag attataacgt gggaaatgat agttacaagg atatgcaact aatgagtctt        720

tgtaaacata atattattgc taatagttct tttagttggt ggggagcatt tttgaataag        780

aatatggaga aaatagttat tgcacctggg aagtggatta atatgaaagg tgtaaaaaaa        840

gtcaatcttt tcccaaagga ttggataatt tattaa                                  876


<210>    19
<211>    462
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of GTP-mannose-1-phosphate
         guanylyltransferase


<400>    19
Met Lys Leu Val Leu Leu Ser Gly Gly Ser Gly Lys Arg Leu Trp Pro
  1               5                  10                  15 

Leu Ser Asn Asp Thr Arg Ser Lys Gln Phe Leu Lys Val Leu Glu Gly
             20                  25                  30 

Arg Asp Gly Gln Lys Glu Ser Met Val Gln Arg Val Trp Ser Gln Ile
         35                  40                  45 

Glu Ala Val Gly Leu Asn Glu Ser Thr Val Ile Ala Thr Ser Lys Leu
     50                  55                  60 

Gln Arg Asp Met Ile Thr Gly Gln Val Gly Thr Asp Ile Pro Leu Ile
 65                  70                  75                  80 

Ile Glu Pro Glu Arg Arg Asp Thr Phe Pro Ala Ile Ala Leu Ala Ala
                 85                  90                  95 

Ser Tyr Leu His Ser Glu Glu Gln Ala Ser Glu Asp Glu Ile Val Val
            100                 105                 110 

Val Leu Pro Val Asp Pro Tyr Val Glu Asp Gly Phe Phe Glu Arg Val
        115                 120                 125 

Lys Asp Leu Glu Ser Val Leu Arg Asp Ser Lys Ala Asp Leu Ala Leu
    130                 135                 140 

Met Gly Val Thr Pro Thr Tyr Pro Ser Ser Lys Tyr Gly Tyr Ile Val
145                 150                 155                 160 

Pro Glu Gly Lys Val Gln Gly Asp Ser Tyr Phe Thr Val Asp Cys Phe
                165                 170                 175 

Thr Glu Lys Pro Ser Glu Glu Ala Ala Val Gly Leu Ile Asn Gln Arg
            180                 185                 190 

Ala Leu Trp Asn Cys Gly Val Phe Ala Phe Arg Leu Gly Tyr Leu Leu
        195                 200                 205 

Gln Ile Leu Gln Glu Lys Lys Leu Pro Leu Gln Phe Glu Thr Leu His
    210                 215                 220 

Lys Glu Tyr Ala Thr Leu Pro Lys Ile Ser Phe Asp Tyr Glu Val Val
225                 230                 235                 240 

Glu Lys Ala Ser His Ile Val Ala Leu Pro Tyr Asp Gly Tyr Trp Lys
                245                 250                 255 

Asp Leu Gly Thr Trp Asn Thr Leu Thr Glu Glu Met Ala Thr Ser Gln
            260                 265                 270 

Ile Gly Lys Gly Val Leu Thr Asp Asp Ser Ala Asn Thr His Leu Val
        275                 280                 285 

Asn Glu Leu Asp Ile Pro Val Thr Val Leu Gly Val Lys Asp Val Val
    290                 295                 300 

Val Ala Ala Ser Pro Asp Gly Ile Leu Val Ala Asp Lys Ala Ser Ser
305                 310                 315                 320 

Pro Lys Ile Lys Glu Ile Ile Gly Asp Trp Asn Tyr Pro Pro Met Tyr
                325                 330                 335 

Glu Glu Arg Leu Trp Gly Trp Ser Arg Val Leu Asp Tyr Ala Lys Tyr
            340                 345                 350 

Asp Glu Glu Thr Glu Met Val Thr Lys Arg Ile His Ile Ser Lys Gly
        355                 360                 365 

Lys Asn Ser Thr Tyr His Tyr His Asn Leu Arg Asp Glu Val Trp Thr
    370                 375                 380 

Ile Val Arg Gly Glu Gly Glu Leu Ala Leu Asp Asp Tyr Ile Arg Arg
385                 390                 395                 400 

Val Lys Ala Gly Asp Ile Ile His Leu Pro Ala Gly Lys Lys His Gly
                405                 410                 415 

Ile His Ala Leu Thr Asp Leu Glu Phe Ile Glu Val Gln Thr Gly Lys
            420                 425                 430 

Val Ile Ser Asn Glu Asp Ser Ile Arg Leu Ile Tyr Asp Trp Asn Asp
        435                 440                 445 

Val Ile Lys Gln Phe Gln Ser Ser Arg Pro Gln Val Ile Leu
    450                 455                 460 




<210>    20
<211>    359
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of phosphomannomurase


<400>    20
Leu Lys Thr Ile Ala Tyr Tyr Ile Ser Asp Tyr Gly Tyr Gly His Ala
  1               5                  10                  15 

Thr Arg Ser Thr Ala Ile Ile Arg Gln Leu Leu Lys Gln Asn Glu Glu
             20                  25                  30 

Ile Glu Ile Ile Ile Cys His Ser Phe Ala Ile Glu Phe Leu Arg Gln
         35                  40                  45 

Ser Phe Arg Tyr Glu Arg Arg Val Arg Phe Arg Glu Ile Leu Thr Asp
     50                  55                  60 

Val Gly Tyr Val Leu Lys Glu Asn Ser Leu Glu Pro Asp Ala Asp Leu
 65                  70                  75                  80 

Leu Asn Arg Gln Val Ala Ser Tyr Val Ser Ser Phe His Thr Lys Leu
                 85                  90                  95 

Met Tyr Glu Lys His Phe Met Lys Val Ala Asn Val Ser Phe Val Ile
            100                 105                 110 

Ser Asp Ile Ser Pro Leu Gly Ile Ala Gly Ala Tyr Leu Leu Lys Leu
        115                 120                 125 

Pro Ser Leu Gly Ile Ser Asn Phe Thr Trp Phe Thr Ala Tyr Glu Gly
    130                 135                 140 

Leu Ile Gly Glu Leu Ser Leu Glu Phe Leu Glu Lys Arg Tyr Lys Gln
145                 150                 155                 160 

Met Thr Tyr Gln Phe Ser Leu Ala Cys Ser Asp Glu Pro His Trp Gly
                165                 170                 175 

Ile Lys Glu Asn Gln Ser Phe Gly Phe Tyr Ser Arg Glu Ile Asn Asp
            180                 185                 190 

Ser Glu Val Gln Arg Ile Arg Lys Glu Ile Asp Pro Glu Gly Glu Ser
        195                 200                 205 

Tyr Ile Ile Tyr Phe Gly Leu Gly Met Lys Val Asp Ile Gly Thr Leu
    210                 215                 220 

Ser Asp Leu Pro Ile Trp Gln Ser Pro Asn Cys Lys Phe Ile Val Ser
225                 230                 235                 240 

Ser Asn Val Asp Val Thr His Pro Asn Val Tyr Lys Ile Pro Gln Asp
                245                 250                 255 

Glu Thr Glu Thr Gln His Tyr Val Ala Ala Ala Asp Leu Gly Leu Thr
            260                 265                 270 

Lys Ala Gly Trp Gly Thr Ile Ser Glu Ala Val Cys Ala Gly Val Pro
        275                 280                 285 

Leu Leu Ile Thr Asn Arg Ser Ser Met Lys Glu Asp Arg His Thr Ile
    290                 295                 300 

Asp Tyr Leu Val Arg His Gln Leu Cys Asp Leu Ile Glu Trp Lys Glu
305                 310                 315                 320 

Leu Glu Gly Leu Val Val Thr Pro Ser Leu Ile Glu Thr Cys Arg Arg
                325                 330                 335 

Gln Gln Ile Asn Arg Tyr Lys Asn Glu Ala Met His Ile Ala Glu Glu
            340                 345                 350 

Ile Asn Lys Lys Ile Asn Ser
        355                


<210>    21
<211>    313
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of GDP-L-fucose synthase


<400>    21
Met Asn Lys Asn Ser Lys Ile Tyr Val Ala Gly His Arg Gly Leu Val
  1               5                  10                  15 

Gly Ser Ala Ile Val Arg Lys Leu Gln Glu Lys Gly Tyr Ser Asn Leu
             20                  25                  30 

Val Tyr Arg Thr Ser Ala Glu Leu Asp Leu Arg Asp Lys Pro Thr Val
         35                  40                  45 

Asp Ala Phe Phe Ala Glu Glu Lys Pro Glu Phe Val Phe Leu Ala Ala
     50                  55                  60 

Ala Lys Val Gly Gly Ile Val Ala Asn Asn Glu Tyr Pro Ala Glu Phe
 65                  70                  75                  80 

Ile His Asp Asn Leu Leu Ile Gln Ser Asn Ile Ile Asp Ala Ala Tyr
                 85                  90                  95 

Arg Asn Asp Val Glu Lys Leu Leu Phe Leu Gly Ser Thr Cys Ile Tyr
            100                 105                 110 

Pro Lys Leu Ala Pro Gln Pro Leu Lys Glu Glu Tyr Leu Leu Thr Gly
        115                 120                 125 

Glu Leu Glu Pro Thr Asn Glu Pro Tyr Ala Ile Ala Lys Ile Ala Gly
    130                 135                 140 

Ile Lys Met Cys Glu Ser Tyr Asn Arg Gln Tyr Gly Thr Lys Tyr Ile
145                 150                 155                 160 

Ser Ala Met Pro Thr Asn Leu Tyr Gly Pro Asn Asp Asn Phe Asp Leu
                165                 170                 175 

His Thr Ser His Val Leu Pro Ala Leu Leu Arg Lys Phe His Glu Ala
            180                 185                 190 

Lys Glu Ser Asn Ala Pro Phe Val Glu Met Trp Gly Thr Gly Thr Pro
        195                 200                 205 

Lys Arg Glu Phe Leu Tyr Ser Asp Asp Leu Ala Asp Ala Cys Val Phe
    210                 215                 220 

Leu Met Asn Thr Tyr Glu Gly Asn Glu Ile Val Asn Ile Gly Val Gly
225                 230                 235                 240 

Glu Asp Ile Thr Ile Lys Glu Leu Gly Glu Lys Val Lys Ala Val Val
                245                 250                 255 

Gly Tyr Glu Gly Asn Ile Gln Phe Asp Thr Ser Lys Pro Asp Gly Thr
            260                 265                 270 

Pro Arg Lys Leu Val Asp Val Thr Lys Leu Asn Ser Leu Gly Trp Lys
        275                 280                 285 

Ala Glu Thr Ala Leu Glu Asp Gly Leu Gln Lys Ala Tyr Gln Trp Phe
    290                 295                 300 

Leu Asp Asn Glu Leu Ala Ala Ala Lys
305                 310            


<210>    22
<211>    359
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of GDP-D-mannose-4,6-dehydratase


<400>    22
Met Lys Thr Ala Leu Ile Thr Gly Val Thr Gly Gln Asp Gly Ser Tyr
  1               5                  10                  15 

Leu Ala Glu Leu Leu Leu Glu Lys Gly Tyr Glu Val His Gly Leu Ile
             20                  25                  30 

Arg Arg Ser Ser Ser Tyr Asn Gln Glu Arg Leu Glu Asp Leu Leu Thr
         35                  40                  45 

Glu Glu Ala Ala Ser Ala Leu Leu Asn Asn Ser Asn Phe His Leu His
     50                  55                  60 

Tyr Gly Asp Val Thr Asp Ala Leu Asn Val Thr Arg Ile Ile Gly Asp
 65                  70                  75                  80 

Ile Gln Pro Asp Glu Ile Tyr Asn Leu Ala Ala Gln Ser His Val Arg
                 85                  90                  95 

Val Ser Phe Asp Met Pro Gly Tyr Thr Leu Asp Val Asp Ala Lys Gly
            100                 105                 110 

Thr Leu Asn Ile Leu Glu Ala Val Arg Ile Leu Gly Leu Thr Asp Lys
        115                 120                 125 

Thr Arg Val Tyr Gln Ala Ser Thr Ser Glu Leu Tyr Gly Lys Val Gln
    130                 135                 140 

Glu Val Pro Gln Lys Glu Thr Thr Pro Phe Tyr Pro Arg Ser Pro Tyr
145                 150                 155                 160 

Gly Val Ala Lys Ile Tyr Gly Phe Trp Ile Thr Lys Asn Tyr Arg Glu
                165                 170                 175 

Ser Tyr Ser Met Phe Ala Val Asn Gly Ile Leu Phe Asn His Glu Ser
            180                 185                 190 

Glu Arg Arg Gly Glu Thr Phe Val Thr Arg Lys Ile Thr Leu Ala Ala
        195                 200                 205 

Ser Arg Ile Ala Gln Gly Lys Gln Gln Lys Leu Thr Leu Gly Asn Leu
    210                 215                 220 

Asp Ser Leu Arg Asp Trp Gly Tyr Ala Lys Asp Tyr Val Glu Cys Met
225                 230                 235                 240 

Trp Leu Ile Leu Gln His Asp Lys Pro Glu Asp Phe Val Ile Ala Thr
                245                 250                 255 

Gly Glu Met His Ser Val Arg Glu Phe Val Gln Leu Ala Phe Lys His
            260                 265                 270 

Thr Gly Ile Glu Ile Glu Trp Ser Gly Glu Gly Leu Glu Glu Lys Gly
        275                 280                 285 

Thr Asn Lys Ala Thr Gly Glu Val Ile Val Glu Ile Asp Pro Lys Phe
    290                 295                 300 

Phe Arg Pro Ala Glu Val Asp Gln Leu Leu Gly Asp Pro Thr Lys Ala
305                 310                 315                 320 

Lys Thr Leu Leu Lys Trp Asn Pro Thr Lys Thr Ser Phe Glu Glu Leu
                325                 330                 335 

Val Arg Ile Met Val Glu Ala Asp Met Lys Lys Val Glu Ser Glu Glu
            340                 345                 350 

Lys Ile Lys Val Met Phe Asp
        355                


<210>    23
<211>    1389
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleotide sequence encoding GTP-mannose-1-phosphate
         guanylyltransferase


<400>    23
atgaaacttg tattgttatc gggtggatct ggtaagcgtc tatggccgtt atcaaatgat         60

acgcgttcga agcagttttt aaaggtatta gaaggacgag atggacagaa agagtcaatg        120

gttcagcgcg tctggagtca aattgaagcg gttgggttaa atgagtcaac ggttattgcg        180

acgtctaagc ttcagcgtga tatgattaca ggtcaggttg gcaccgatat tccgttgatt        240

attgaacctg agcgccgcga tacgtttcca gcgattgctt tagctgcttc atatcttcat        300

tccgaagagc aagcatctga agatgaaatc gttgtggtgt tgccagttga cccttatgtg        360

gaagacggat tttttgaacg ggtaaaagat ttagaaagtg tgcttcgtga ttcaaaagcg        420

gatttagcgt taatgggtgt tacacctacg tatccttcat ctaagtacgg gtacatcgtg        480

ccggaaggaa aggtacaagg ggacagttat tttacagtgg attgttttac agaaaagccg        540

agtgaagaag cagctgttgg attaattaat cagcgtgcgc tttggaactg cggcgttttt        600

gcgtttcgtt taggttattt gcttcaaatt ttacaagaga aaaaacttcc acttcaattt        660

gaaacgctac ataaagaata tgcaacattg cctaaaatta gttttgacta tgaagtggtg        720

gaaaaggcaa gtcatattgt cgcgcttccg tatgatgggt actggaaaga tcttggtaca        780

tggaatacgt taacggaaga aatggcgaca tctcaaattg gaaaaggtgt attaacggac        840

gactcagcta atacgcattt agtgaatgaa ttagatattc cggttacggt attaggcgta        900

aaagatgtag ttgttgccgc tagtccagat gggatcttag ttgcggataa agcatctagt        960

cctaagatta aagaaattat tggcgactgg aactaccctc caatgtatga agaacgtctt       1020

tggggctggt cacgcgtgtt agattatgca aagtatgatg aagaaactga aatggtgaca       1080

aaacgtattc atatttcaaa aggaaagaac tcgacgtatc actatcataa tttacgtgat       1140

gaagtatgga cgattgtacg tggagaagga gagcttgcgc ttgatgacta cattcgccgc       1200

gtaaaagcag gggatattat tcacttgccg gctggcaaaa agcacggaat tcatgcgtta       1260

accgaccttg aatttattga agtacaaacg ggaaaagtga tttcaaatga agatagtatt       1320

cgccttattt atgactggaa tgatgtgatc aagcaattcc aatcatcaag accgcaggtg       1380

attttataa                                                               1389


<210>    24
<211>    1080
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleotide sequence encoding phosphomannomurase


<400>    24
ttgaaaacaa ttgcctatta catttcagac tacggatatg gccacgcgac tagaagcacg         60

gccattattc gtcaattatt aaagcaaaat gaagaaatag aaattattat ttgtcattca        120

tttgcgattg agtttctcag acaatctttt agatatgaac gtcgtgtgag gtttcgagag        180

attttaacgg atgtcggcta cgtgctaaaa gaaaattcac tagaaccaga cgctgatttg        240

ttgaatcgtc aagttgcttc ttatgtatca agctttcata cgaagcttat gtatgagaag        300

cactttatga aagtagccaa cgtttcattc gtgatttcgg atatttctcc gttagggatc        360

gcaggtgcct atctattaaa attgccttct ttaggaatat caaatttcac ttggtttaca        420

gcatacgaag ggttaattgg agagctatcg ctggaatttt tagaaaaacg ttataagcag        480

atgacgtatc agttttcgct agcatgtagt gatgagccac attggggtat caaagaaaat        540

caaagctttg gtttttattc aagagaaata aacgattctg aagtacagcg tattcgtaaa        600

gagattgatc ctgaaggtga gagttacatc atctacttcg gtctgggtat gaaagtagat        660

attggaacgt tgtcggactt gcctatttgg caaagtccaa actgcaagtt tatcgtttca        720

tcgaatgttg atgttactca tcccaatgtt tataaaattc ctcaagatga aacagaaacg        780

cagcactatg tagcagcagc tgatcttggt ttaacaaaag caggctgggg cacgattagt        840

gaagcggttt gcgctggtgt gccgctgttg attacaaatc gcagcagcat gaaagaagat        900

cgtcatacga ttgattattt agttcgtcat cagctatgcg acttaataga gtggaaagag        960

ttagaaggtt tagtagtaac tccttcgctt attgagacat gcaggcggca gcaaatcaat       1020

cgctataaaa atgaagcgat gcatatcgcc gaagaaatca ataaaaaaat aaacagttag       1080

                                                                        1080


<210>    25
<211>    942
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleotide sequence encoding GDP-L-fucose synthase


<400>    25
atgaacaaaa attcgaaaat ttacgtagct ggtcatagag gtctcgtcgg ttcagctatc         60

gtacgaaaac tacaagaaaa aggctatagc aatctagtgt atcgcacaag cgctgagcta        120

gatttaagag acaagccgac agtagacgca ttctttgctg aagaaaagcc agaatttgtt        180

ttcttagcag ccgctaaagt aggtggtatt gtggcaaaca atgaatatcc agcggaattt        240

attcacgata acttattgat tcaatcaaat attattgatg cagcatatcg caatgatgta        300

gagaaactat tattcctagg aagtacatgt atttatccaa aattagcgcc tcagccatta        360

aaagaagaat acttactaac aggtgagctg gagccaacaa acgaaccgta tgcaattgct        420

aaaattgctg gaatcaaaat gtgtgaatcc tataaccgtc agtacggaac aaaatatatc        480

tcggcaatgc ctactaacct ttacggtcca aacgacaatt ttgaccttca tacatcacac        540

gttcttcctg cactgcttcg taagttccat gaagcgaaag aaagcaatgc gccgtttgtt        600

gaaatgtggg gaacaggcac accaaaacgt gaatttctat attcagatga cctagcagat        660

gcgtgcgtat tcttaatgaa tacatatgaa ggcaatgaaa tcgttaatat cggtgtggga        720

gaagatatta cgattaaaga acttggcgaa aaagtaaaag cagttgttgg ttatgaagga        780

aatattcagt ttgatacgtc aaaaccagac ggaacgcctc gtaaactagt agacgtaaca        840

aaattgaaca gtcttggctg gaaagcagaa acagctttag aagacggtct tcaaaaagcg        900

tatcagtggt ttttagataa cgaactagct gcagcaaaat aa                           942


<210>    26
<211>    1080
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleotide sequence encoding GDP-D-mannose-4,6-dehydratase


<400>    26
atgaaaacag cattaattac aggtgtaaca ggacaagatg gatcatactt agcagaactt         60

ttactagaaa aaggctatga agtgcacgga ctaattcgcc gcagctcttc ttataaccaa        120

gagcgtctag aagatctttt aacagaagaa gcagcgagcg cattattaaa caactcaaac        180

ttccaccttc actatggtga cgtaacagat gcattaaatg ttactcgtat tattggtgac        240

attcagccag atgaaattta caatctagct gcacagtcac atgtacgtgt gtcttttgat        300

atgcctggat atacattaga tgttgatgca aaaggtacgt taaacattct tgaagctgta        360

cgtattttag ggttaacaga taaaacacgt gtgtaccaag cttctacatc tgaactgtac        420

ggaaaagttc aagaagtacc acaaaaggaa acaacacctt tctaccctcg ttctccatat        480

ggcgtagcga aaatttacgg cttttggatt acaaagaact accgtgaatc ttatagcatg        540

tttgcagtaa acggtatttt atttaaccat gaatcagagc gccgcggcga aacgttcgta        600

acacgtaaaa ttacgcttgc tgcttcacgt atcgcgcaag gcaagcagca aaaattaacg        660

cttggaaact tagactcttt acgtgactgg ggttacgcaa aagactatgt agaatgcatg        720

tggttaattc ttcaacacga taaacctgaa gattttgtta ttgcgactgg tgaaatgcac        780

tcagtacgtg aatttgtaca gcttgcattc aaacatacag gcattgaaat tgagtggagc        840

ggtgaaggtc tagaagaaaa aggaacaaat aaagcaacgg gtgaagtgat cgtagaaatc        900

gatcctaagt tcttccgtcc tgcagaagtg gatcaattgt taggtgatcc aactaaagcc        960

aagactttat taaagtggaa ccccactaag acttcgtttg aagaattagt tcgcattatg       1020

gtggaagcgg acatgaagaa ggttgaaagc gaagagaaga ttaaggtgat gtttgattaa       1080

                                                                        1080


<210>    27
<211>    587
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of lactose permease Lac12


<400>    27
Met Ala Asp His Ser Ser Ser Ser Ser Ser Leu Gln Lys Lys Pro Ile
  1               5                  10                  15 

Asn Thr Ile Glu His Lys Asp Thr Leu Gly Asn Asp Arg Asp His Lys
             20                  25                  30 

Glu Ala Leu Asn Ser Asp Asn Asp Asn Thr Ser Gly Leu Lys Ile Asn
         35                  40                  45 

Gly Val Pro Ile Glu Asp Ala Arg Glu Glu Val Leu Leu Pro Gly Tyr
     50                  55                  60 

Leu Ser Lys Gln Tyr Tyr Lys Leu Tyr Gly Leu Cys Phe Ile Thr Tyr
 65                  70                  75                  80 

Leu Cys Ala Thr Met Gln Gly Tyr Asp Gly Ala Leu Met Gly Ser Ile
                 85                  90                  95 

Tyr Thr Glu Asp Ala Tyr Leu Lys Tyr Tyr His Leu Asp Ile Asn Ser
            100                 105                 110 

Ser Ser Gly Thr Gly Leu Val Phe Ser Ile Phe Asn Val Gly Gln Ile
        115                 120                 125 

Cys Gly Ala Phe Phe Val Pro Leu Met Asp Trp Lys Gly Arg Lys Pro
    130                 135                 140 

Ala Ile Leu Ile Gly Cys Leu Gly Val Val Ile Gly Ala Ile Ile Ser
145                 150                 155                 160 

Ser Leu Thr Thr Thr Lys Ser Ala Leu Ile Gly Gly Arg Trp Phe Val
                165                 170                 175 

Ala Phe Phe Ala Thr Ile Ala Asn Ala Ala Ala Pro Thr Tyr Cys Ala
            180                 185                 190 

Glu Val Ala Pro Ala His Leu Arg Gly Lys Val Ala Gly Leu Tyr Asn
        195                 200                 205 

Thr Leu Trp Ser Val Gly Ser Ile Val Ala Ala Phe Ser Thr Tyr Gly
    210                 215                 220 

Thr Asn Lys Asn Phe Pro Asn Ser Ser Lys Ala Phe Lys Ile Pro Leu
225                 230                 235                 240 

Tyr Leu Gln Met Met Phe Pro Gly Leu Val Cys Ile Phe Gly Trp Leu
                245                 250                 255 

Ile Pro Glu Ser Pro Arg Trp Leu Val Gly Val Gly Arg Glu Glu Glu
            260                 265                 270 

Ala Arg Glu Phe Ile Ile Lys Tyr His Leu Asn Gly Asp Arg Thr His
        275                 280                 285 

Pro Leu Leu Asp Met Glu Met Ala Glu Ile Ile Glu Ser Phe His Gly
    290                 295                 300 

Thr Asp Leu Ser Asn Pro Leu Glu Met Leu Asp Val Arg Ser Leu Phe
305                 310                 315                 320 

Arg Thr Arg Ser Asp Arg Tyr Arg Ala Met Leu Val Ile Leu Met Ala
                325                 330                 335 

Trp Phe Gly Gln Phe Ser Gly Asn Asn Val Cys Ser Tyr Tyr Leu Pro
            340                 345                 350 

Thr Met Leu Arg Asn Val Gly Met Lys Ser Val Ser Leu Asn Val Leu
        355                 360                 365 

Met Asn Gly Val Tyr Ser Ile Val Thr Trp Ile Ser Ser Ile Cys Gly
    370                 375                 380 

Ala Phe Phe Ile Asp Lys Ile Gly Arg Arg Glu Gly Phe Leu Gly Ser
385                 390                 395                 400 

Ile Ser Gly Ala Ala Leu Ala Leu Thr Gly Leu Ser Ile Cys Thr Ala
                405                 410                 415 

Arg Tyr Glu Lys Thr Lys Lys Lys Ser Ala Ser Asn Gly Ala Leu Val
            420                 425                 430 

Phe Ile Tyr Leu Phe Gly Gly Ile Phe Ser Phe Ala Phe Thr Pro Met
        435                 440                 445 

Gln Ser Met Tyr Ser Thr Glu Val Ser Thr Asn Leu Thr Arg Ser Lys
    450                 455                 460 

Ala Gln Leu Leu Asn Phe Val Val Ser Gly Val Ala Gln Phe Val Asn
465                 470                 475                 480 

Gln Phe Ala Thr Pro Lys Ala Met Lys Asn Ile Lys Tyr Trp Phe Tyr
                485                 490                 495 

Val Phe Tyr Val Phe Phe Asp Ile Phe Glu Phe Ile Val Ile Tyr Phe
            500                 505                 510 

Phe Phe Val Glu Thr Lys Gly Arg Ser Leu Glu Glu Leu Glu Val Val
        515                 520                 525 

Phe Glu Ala Pro Asn Pro Arg Lys Ala Ser Val Asp Gln Ala Phe Leu
    530                 535                 540 

Ala Gln Val Arg Ala Thr Leu Val Gln Arg Asn Asp Val Arg Val Ala
545                 550                 555                 560 

Asn Ala Gln Asn Leu Lys Glu Gln Glu Pro Leu Lys Ser Asp Ala Asp
                565                 570                 575 

His Val Glu Lys Leu Ser Glu Ala Glu Ser Val
            580                 585        


<210>    28
<211>    413
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of lactose permease LacY


<400>    28
Met Lys Ser Ser Lys Ser Leu Tyr Trp Lys Leu Ser Ala Tyr Phe Phe
  1               5                  10                  15 

Phe Phe Phe Phe Thr Trp Ser Ser Ser Tyr Ser Leu Phe Ser Ile Trp
             20                  25                  30 

Leu Gly Gln Glu Ile Lys Leu Asn Gly Ser Ala Thr Gly Leu Ile Phe
         35                  40                  45 

Ser Val Asn Ala Ile Phe Ala Leu Cys Met Gln Pro Leu Tyr Gly Tyr
     50                  55                  60 

Ile Ser Asp Arg Ile Gly Leu Lys Lys His Val Leu Phe Phe Ile Ser
 65                  70                  75                  80 

Cys Leu Leu Val Phe Val Gly Pro Phe Tyr Ile Phe Val Tyr Gly Pro
                 85                  90                  95 

Leu Leu Gln Tyr Asn Val Val Ile Gly Ala Ile Ile Gly Gly Leu Tyr
            100                 105                 110 

Leu Gly Val Ala Phe Leu Ala Gly Ile Gly Ala Ile Glu Thr Tyr Ile
        115                 120                 125 

Glu Lys Val Ser Arg Lys Tyr Lys Phe Glu Tyr Gly Lys Ser Arg Met
    130                 135                 140 

Trp Gly Ser Leu Gly Trp Ala Ala Ala Thr Phe Phe Ala Gly Gln Leu
145                 150                 155                 160 

Phe Asn Ile Asn Pro His Ile Asn Phe Trp Val Ala Ser Val Ser Ala
                165                 170                 175 

Val Ile Leu Val Ala Ile Ile Phe Ser Val Lys Val Glu Met Ser Ser
            180                 185                 190 

Tyr Glu Met Glu Lys Ala Glu Ser Val Thr Leu Arg Asp Val Gly Ser
        195                 200                 205 

Leu Phe Leu Leu Lys Glu Phe Trp Phe Phe Met Ile Tyr Val Val Gly
    210                 215                 220 

Val Thr Cys Val Tyr Gly Val Tyr Asp Gln Gln Phe Pro Ile Tyr Tyr
225                 230                 235                 240 

Ala Ser Leu Phe Pro Thr Glu Ser Ile Gly Asn Gln Val Phe Gly Tyr
                245                 250                 255 

Leu Asn Ser Phe Gln Val Phe Leu Glu Ala Gly Met Met Phe Ala Ala
            260                 265                 270 

Pro Phe Ile Val Asn Lys Ile Gly Ala Lys Asn Ser Leu Ile Leu Ala
        275                 280                 285 

Gly Phe Leu Met Gly Phe Arg Ile Ile Gly Ser Gly Leu Val Val Gly
    290                 295                 300 

Pro Ile Gly Ile Ser Ser Met Lys Leu Ile His Ala Leu Glu Leu Pro
305                 310                 315                 320 

Ile Met Leu Ile Ala Ile Phe Lys Tyr Leu Ala Ala Asn Phe Asp Thr
                325                 330                 335 

Arg Leu Ser Ser Ile Leu Tyr Leu Val Gly Phe Gln Phe Ala Ser Gln
            340                 345                 350 

Ile Gly Ala Ser Val Leu Ser Pro Ile Val Gly Gly Leu Tyr Asp Ser
        355                 360                 365 

Val Gly Phe Ser Arg Thr Tyr Leu Ile Met Gly Gly Met Val Leu Val
    370                 375                 380 

Phe Asn Val Ile Ser Met Phe Thr Leu Leu Asn Ser Lys Lys His Arg
385                 390                 395                 400 

Phe Ile Arg Lys Asp Val Gln Glu Asn Thr Gln Ile Ile
                405                 410            


<210>    29
<211>    1764
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleotide sequence encoding lactose permease Lac12


<400>    29
atggctgatc actcttcttc ttcttcttct ttacaaaaaa aaccaattaa cacaattgaa         60

cacaaagata cattaggtaa cgatcgtgat cacaaagaag ctttaaactc tgataacgat        120

aacacatctg gtttaaaaat taacggtgta ccaattgaag atgctcgtga agaagtatta        180

ttaccaggtt acttatctaa acaatactac aaattatacg gtttatgttt cattacatac        240

ttatgtgcta caatgcaagg ttacgatggt gctttaatgg gttctattta cacagaagat        300

gcttacttaa aatactacca cttagatatt aactcttctt ctggtacagg tttagtattc        360

tctattttca acgtaggtca aatttgtggt gctttcttcg taccattaat ggattggaaa        420

ggtcgtaaac cagctatttt aattggttgt ttaggtgtag taattggtgc tattatttct        480

tctttaacaa caacaaaatc tgctttaatt ggtggtcgtt ggttcgtagc tttcttcgct        540

acaattgcta acgctgctgc tccaacatac tgtgctgaag tagctccagc tcacttacgt        600

ggtaaagtag ctggtttata caacacatta tggtctgtag gttctattgt agctgctttc        660

tctacatacg gtacaaacaa aaacttccca aactcttcta aagctttcaa aattccatta        720

tacttacaaa tgatgttccc aggtttagta tgtattttcg gttggttaat tccagaatct        780

ccacgttggt tagtaggtgt aggtcgtgaa gaagaagctc gtgaattcat tattaaatac        840

cacttaaacg gtgatcgtac acacccatta ttagatatgg aaatggctga aattattgaa        900

tctttccacg gtacagattt atctaaccca ttagaaatgt tagatgtacg ttctttattc        960

cgtacacgtt ctgatcgtta ccgtgctatg ttagtaattt taatggcttg gttcggtcaa       1020

ttctctggta acaacgtatg ttcttactac ttaccaacaa tgttacgtaa cgtaggtatg       1080

aaatctgtat ctttaaacgt attaatgaac ggtgtatact ctattgtaac atggatttct       1140

tctatttgtg gtgctttctt cattgataaa attggtcgtc gtgaaggttt cttaggttct       1200

atttctggtg ctgctttagc tttaacaggt ttatctattt gtacagctcg ttacgaaaaa       1260

acaaaaaaaa aatctgcttc taacggtgct ttagtattca tttacttatt cggtggtatt       1320

ttctctttcg ctttcacacc aatgcaatct atgtactcta cagaagtatc tacaaactta       1380

acacgttcta aagctcaatt attaaacttc gtagtatctg gtgtagctca attcgtaaac       1440

caattcgcta caccaaaagc tatgaaaaac attaaatact ggttctacgt attctacgta       1500

ttcttcgata ttttcgaatt cattgtaatt tacttcttct tcgtagaaac aaaaggtcgt       1560

tctttagaag aattagaagt agtattcgaa gctccaaacc cacgtaaagc ttctgtagat       1620

caagctttct tagctcaagt acgtgctaca ttagtacaac gtaacgatgt acgtgtagct       1680

aacgctcaaa acttaaaaga acaagaacca ttaaaatctg atgctgatca cgtagaaaaa       1740

ttatctgaag ctgaatctgt ataa                                              1764


<210>    30
<211>    1242
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleotide sequence encoding lactose permease LacY


<400>    30
atgaaaagtt caaaaagttt atattggaag ctgagtgcat attttttctt cttctttttt         60

acgtggtcgt ccagctactc tttattttct atctggttag ggcaagagat taagttgaac        120

ggatcagcaa caggcttaat cttttctgtg aacgctatct ttgctttgtg tatgcagcct        180

ttgtacggat atatatctga caggataggt ttaaagaaac acgttttatt ttttattagc        240

tgtcttttag tgtttgtagg accattttat atttttgttt atggaccttt gcttcaatac        300

aatgtggtga taggggctat tattggaggc ttatatttag gagtagcgtt tttggcgggc        360

atcggagcga ttgaaacata catagagaaa gtcagccgaa aatataaatt tgagtatgga        420

aaatctagaa tgtgggggtc attaggctgg gctgcagcta cgttttttgc aggacaatta        480

tttaatatta atcctcacat taatttttgg gtggcatctg tttcagcggt tatcctagtc        540

gctattattt tttctgtaaa agtagaaatg agcagttatg aaatggaaaa agctgaatcc        600

gtcacgctaa gagatgtagg tagtttattt ttattgaagg aattttggtt ttttatgatt        660

tatgttgttg gtgttacatg cgtatatggg gtatacgatc agcaatttcc tatctattat        720

gcatctttgt ttcctactga atcgataggt aaccaagtat ttggctactt aaattcgttt        780

caagtatttt tagaagctgg aatgatgttc gcagcaccgt ttattgtaaa taagatcggt        840

gcaaaaaata gtttaatttt agcaggattt ctaatgggat ttcgtattat tgggtcaggg        900

cttgttgttg gtccaatcgg aatatcttca atgaaactca ttcatgcatt agaacttcct        960

attatgctca ttgccatctt taaatatttg gcagctaact ttgacacgcg cctatcttcc       1020

atcctttatt tggttggatt ccaatttgct tcgcaaatag gtgcttcagt tctttcaccc       1080

atagtgggag gattatatga tagcgtaggt ttttcccgta cctatttaat tatgggaggt       1140

atggttttgg tatttaacgt tatttcgatg tttacactac taaactctaa aaaacataga       1200

tttataagaa aagatgtaca agaaaacacg cagattatat ag                          1242


<210>    31
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Te2FT_F


<400>    31
catatgatta tcgttcatct gtg                                                 23


<210>    32
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Te2FT_R


<400>    32
ctcgagtcac agaacaatcc acc                                                 23


<210>    33
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Bt2FT_F


<400>    33
catatgaacg aaatccacgt gct                                                 23


<210>    34
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Bt2FT_1_R


<400>    34
ctcgagtcac tggccagccg gaatc                                               25


<210>    35
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Bm2FT_F


<400>    35
catatgaaaa ttgttcagat ttct                                                24


<210>    36
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Bm2FT_R


<400>    36
ctcgagtcag taaacgatcc aacct                                               25


<210>    37
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Lg2FT_F


<400>    37
catatgctgt acgttgaaat ggacg                                               25


<210>    38
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Lg2FT_R


<400>    38
ctcgagttac gcgtcgatca ggatc                                               25


<210>    39
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Ls2FT_F


<400>    39
catatggaag agattcacac cctg                                                24


<210>    40
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Ls2FT_R


<400>    40
ctcgagctaa cggccggcag gtac                                                24


<210>    41
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Ll2FT_F


<400>    41
catatgtcta aaaacattta tgtac                                               25


<210>    42
<211>    26
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Ll2FT_R


<400>    42
ctcgagttag attttgatcc agttgt                                              26


<210>    43
<211>    39
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Am2FT_1_F


<400>    43
aagaaggaga tatacatatg atggctggac attcttgcg                                39


<210>    44
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Am2FT_1_R


<400>    44
tggtggtggt ggtgctcgag ttaaatgcgc agccatgagc                               40


<210>    45
<211>    39
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Am2FT_2_F


<400>    45
aagaaggaga tatacatatg atgaacatgg aacgtaaga                                39


<210>    46
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Am2FT_2_R


<400>    46
tggtggtggt ggtgctcgag ttacagggtc acccagtgcg                               40


<210>    47
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Fp2FT_F


<400>    47
aagaaggaga tatacatatg atgatttacg tggaaatg                                 38


<210>    48
<211>    39
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Fp2FT_R


<400>    48
tggtggtggt ggtgctcgag ttattcgtta acgatgtta                                39


<210>    49
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Ri2FT_F


<400>    49
aagaaggaga tatacatatg atgatcgcag ttaagatc                                 38


<210>    50
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Ri2FT_R


<400>    50
tggtggtggt ggtgctcgag ttatttcagg ataatccagt                               40


<210>    51
<211>    39
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Bm2FT_2_F


<400>    51
aagaaggaga tatacatatg ggttgcatta aacgcctgt                                39


<210>    52
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Bm2FT_2_R


<400>    52
tggtggtggt ggtgctcgag ctattccagg tgagtgttca                               40


<210>    53
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Bm2FT_3_F


<400>    53
aagaaggaga tatacatatg atcattgtcc agattaaa                                 38


<210>    54
<211>    41
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Bm2FT_3_R


<400>    54
tggtggtggt ggtgctcgag ctattgaaga tccgctttga t                             41


<210>    55
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Bm2FT_4_F


<400>    55
aagaaggaga tatacatatg atcattgtgc gtgttatc                                 38


<210>    56
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Bm2FT_4_R


<400>    56
tggtggtggt ggtgctcgag ttagaacgta ttaatacact                               40


<210>    57
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Bs2FT_F


<400>    57
aagaaggaga tatacatatg aaaattgtaa aagttatc                                 38


<210>    58
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Bs2FT_R


<400>    58
tggtggtggt ggtgctcgag ttagtagatg atccagtctt                               40


<210>    59
<211>    36
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Gs2FT_F


<400>    59
aagaaggaga tatacatatg aaaattgtaa aagtta                                   36


<210>    60
<211>    39
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer Gs2FT_R


<400>    60
tggtggtggt ggtgctcgag ttagatttta atccattct                                39


<210>    61
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer TAG_PET24ma_F


<400>    61
tagctcgagc accaccacca ccacc                                               25


<210>    62
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer TAA_PET24ma_F


<400>    62
taactcgagc accaccacca ccacc                                               25


<210>    63
<211>    40
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    primer PET24ma_R


<400>    63
catatgtata tctccttctt aaagttaaac aaaattattt                               40
