               SEQUENCE LISTING

<110> Encodia, Inc.

<120> METALLOENZYMES FOR BIOMOLECULAR RECOGNITION OF N-TERMINAL MODIFIED PEPTIDES

<130> 4614-2002940

<150>  63/250,199
<151>  2021-09-29

<150>  63/133,166
<151>  2020-12-31

<160> 66

<170> PatentIn version 3.5

<210> 1
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> P5 primer

<400> 1
aatgatacgg cgaccaccga                                                20


<210> 2
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> P7 primer

<400> 2
caagcagaag acggcatacg agat                                           24


<210> 3
<211> 723
<212> PRT
<213> Thermomonas hydrothermalis


<220> 
<223> Dipeptidyl peptidase from Thermomonas hydrothermalis (with the
      signal peptide)

<400> 3
Met His Lys Thr Arg Leu Val Ala Ala Leu Ala Ala Ala Leu Ala Thr 
1               5                   10                  15      
Leu Ala Pro Ala Ala Trp Ala Asp Glu Gly Met Trp Val Pro Gln Gln 
            20                  25                  30          
Leu Pro Glu Ile Ala Gly Ala Leu Lys Lys Ala Gly Leu Lys Leu Asp 
        35                  40                  45              
Pro Lys Gln Leu Ser Asp Leu Thr Gly Asp Pro Met Gly Ala Val Val 
    50                  55                  60                  
Ser Leu Gly Gly Cys Thr Gly Ser Phe Val Ser Pro Gln Gly Leu Val 
65                  70                  75                  80  
Ala Thr Asn His His Cys Ala Tyr Gly Ala Ile Gln Leu Asn Ser Thr 
                85                  90                  95      
Pro Glu Lys Asn Leu Ile Lys Asp Gly Phe Asn Ala Pro Thr Gln Ala 
            100                 105                 110         
Asp Glu Leu Ser Ala Gly Pro Asn Ala Arg Ile Tyr Val Leu Glu Gly 
        115                 120                 125             
Ile Thr Asp Val Thr Ala Gln Ala Lys Ala Ala Met Ala Ala Ala Gly 
    130                 135                 140                 
Asn Asp Pro Val Ala Arg Ala Asn Ala Leu Glu Ala Phe Glu Lys Lys 
145                 150                 155                 160 
Ile Thr Ser Asp Cys Glu Ala Glu Pro Gly Tyr Arg Cys Arg Val Tyr 
                165                 170                 175     
Ser Phe Met Gly Gly Ile Thr Tyr Arg Leu Phe Lys Asn Leu Glu Ile 
            180                 185                 190         
Lys Asp Val Arg Leu Val Tyr Ala Pro Pro Ser Ser Val Gly Lys Phe 
        195                 200                 205             
Gly Gly Asp Ile Asp Asn Trp Met Trp Pro Arg His Thr Gly Asp Phe 
    210                 215                 220                 
Ser Phe Tyr Arg Ala Tyr Val Gly Lys Asp Gly Lys Pro Ala Pro Tyr 
225                 230                 235                 240 
Ser Lys Asp Asn Val Pro Tyr Arg Pro Lys His Trp Leu Lys Ile Ala 
                245                 250                 255     
Asp Thr Pro Leu Gly Glu Gly Asp Phe Val Met Val Ala Gly Tyr Pro 
            260                 265                 270         
Gly Arg Thr Asp Arg Tyr Ala Leu Val Ala Glu Phe Glu Asn Thr Gln 
        275                 280                 285             
Asn Trp Leu Tyr Pro Ala Ile Ser Lys Ala Tyr Lys Asp Gln Ile Ala 
    290                 295                 300                 
Leu Val Glu Ala Ala Ala Lys Asp Asn Pro Glu Ile Ala Val Lys Tyr 
305                 310                 315                 320 
Ala Ala Ala Leu Ala Gly Trp Asn Asn Thr Ser Lys Asn Phe Asp Gly 
                325                 330                 335     
Gln Leu Glu Gly Phe Lys Arg Asn Asp Val Leu Ala Ile Lys Arg Arg 
            340                 345                 350         
Glu Glu Ala Ala Val Leu Glu Trp Leu Arg Ala Arg Gly Lys Ala Gly 
        355                 360                 365             
Thr Pro Ala Leu Glu Ala His Ala Ala Leu Val Lys Leu Val Ala Asp 
    370                 375                 380                 
Thr Ala Arg Thr Gln Glu Arg Asp Leu Val Leu Gly Ser Phe Asn Arg 
385                 390                 395                 400 
Thr Gly Ile Ile Gly Val Ala Val Asn Leu Tyr Arg Leu Ala Ile Glu 
                405                 410                 415     
Arg Gln Lys Pro Asp Ala Glu Arg Glu Pro Gly Tyr Gln Gln Arg Asp 
            420                 425                 430         
Leu Pro Val Ile Glu Gly Ser Leu Lys Gln Met Glu Arg Arg Tyr Val 
        435                 440                 445             
Pro Ala Met Asp Arg Gln Leu Arg Ala Tyr Trp Leu Asp Arg Tyr Val 
    450                 455                 460                 
Ala Leu Pro Ala Ala Gln His Val Ala Ala Val Asp Ala Trp Leu Gly 
465                 470                 475                 480 
Gly Ser Asp Lys Ala Ala Ala Glu Ala Ala Leu Ala Arg Leu Asp Gln 
                485                 490                 495     
Ser Arg Leu Gly Ser Leu Glu Glu Arg Leu Lys Trp Phe Asn Ala Asp 
            500                 505                 510         
Arg Ala Ala Phe Glu Ala Ser Thr Asp Pro Ala Ile Gln Tyr Ala Val 
        515                 520                 525             
Ala Val Met Pro Thr Leu Leu Ala Met Glu Gln Gln Ala Lys Thr Arg 
    530                 535                 540                 
Tyr Gly Val Ala Leu Glu Ala Arg Pro Arg Tyr Leu Gln Ala Val Val 
545                 550                 555                 560 
Asp Tyr Lys Lys Ser Lys Gly Gln Ala Val Tyr Pro Asp Ala Asn Ser 
                565                 570                 575     
Thr Leu Arg Ile Thr Tyr Gly His Val Lys Gly Tyr Thr Gly Leu Asn 
            580                 585                 590         
Gly Lys Val Tyr Thr Pro Phe Thr Thr Leu Glu Glu Val Ala Ala Lys 
        595                 600                 605             
Glu Thr Gly Val Glu Pro Phe Asp Asn Pro Lys Ala Leu Leu Glu Ala 
    610                 615                 620                 
Val Ala Ala Lys Arg Tyr Ala Gly Leu Ala Asp Ala Arg Leu Gly Thr 
625                 630                 635                 640 
Val Pro Val Asn Phe Leu Ala Asp Leu Asp Ile Thr Gly Gly Asn Ser 
                645                 650                 655     
Gly Ser Pro Val Leu Asp Ala Asn Gly Arg Leu Val Gly Leu Ala Phe 
            660                 665                 670         
Asp Gly Thr Leu Glu Ser Val Ala Ser Asn Trp Val Phe Asp Pro Val 
        675                 680                 685             
Leu Thr Arg Met Ile Ser Val Asp Gln Arg Tyr Met Arg Trp Ile Met 
    690                 695                 700                 
Gln Glu Val Met Pro Ala Pro Gln Leu Leu Glu Glu Leu Gly Val Pro 
705                 710                 715                 720 
Pro Arg Gln 
            

<210> 4
<211> 700
<212> PRT
<213> Thermomonas hydrothermalis


<220> 
<223> Dipeptidyl peptidase from Thermomonas hydrothermalis (without the
      signal peptide)

<400> 4
Asp Glu Gly Met Trp Val Pro Gln Gln Leu Pro Glu Ile Ala Gly Ala 
1               5                   10                  15      
Leu Lys Lys Ala Gly Leu Lys Leu Asp Pro Lys Gln Leu Ser Asp Leu 
            20                  25                  30          
Thr Gly Asp Pro Met Gly Ala Val Val Ser Leu Gly Gly Cys Thr Gly 
        35                  40                  45              
Ser Phe Val Ser Pro Gln Gly Leu Val Ala Thr Asn His His Cys Ala 
    50                  55                  60                  
Tyr Gly Ala Ile Gln Leu Asn Ser Thr Pro Glu Lys Asn Leu Ile Lys 
65                  70                  75                  80  
Asp Gly Phe Asn Ala Pro Thr Gln Ala Asp Glu Leu Ser Ala Gly Pro 
                85                  90                  95      
Asn Ala Arg Ile Tyr Val Leu Glu Gly Ile Thr Asp Val Thr Ala Gln 
            100                 105                 110         
Ala Lys Ala Ala Met Ala Ala Ala Gly Asn Asp Pro Val Ala Arg Ala 
        115                 120                 125             
Asn Ala Leu Glu Ala Phe Glu Lys Lys Ile Thr Ser Asp Cys Glu Ala 
    130                 135                 140                 
Glu Pro Gly Tyr Arg Cys Arg Val Tyr Ser Phe Met Gly Gly Ile Thr 
145                 150                 155                 160 
Tyr Arg Leu Phe Lys Asn Leu Glu Ile Lys Asp Val Arg Leu Val Tyr 
                165                 170                 175     
Ala Pro Pro Ser Ser Val Gly Lys Phe Gly Gly Asp Ile Asp Asn Trp 
            180                 185                 190         
Met Trp Pro Arg His Thr Gly Asp Phe Ser Phe Tyr Arg Ala Tyr Val 
        195                 200                 205             
Gly Lys Asp Gly Lys Pro Ala Pro Tyr Ser Lys Asp Asn Val Pro Tyr 
    210                 215                 220                 
Arg Pro Lys His Trp Leu Lys Ile Ala Asp Thr Pro Leu Gly Glu Gly 
225                 230                 235                 240 
Asp Phe Val Met Val Ala Gly Tyr Pro Gly Arg Thr Asp Arg Tyr Ala 
                245                 250                 255     
Leu Val Ala Glu Phe Glu Asn Thr Gln Asn Trp Leu Tyr Pro Ala Ile 
            260                 265                 270         
Ser Lys Ala Tyr Lys Asp Gln Ile Ala Leu Val Glu Ala Ala Ala Lys 
        275                 280                 285             
Asp Asn Pro Glu Ile Ala Val Lys Tyr Ala Ala Ala Leu Ala Gly Trp 
    290                 295                 300                 
Asn Asn Thr Ser Lys Asn Phe Asp Gly Gln Leu Glu Gly Phe Lys Arg 
305                 310                 315                 320 
Asn Asp Val Leu Ala Ile Lys Arg Arg Glu Glu Ala Ala Val Leu Glu 
                325                 330                 335     
Trp Leu Arg Ala Arg Gly Lys Ala Gly Thr Pro Ala Leu Glu Ala His 
            340                 345                 350         
Ala Ala Leu Val Lys Leu Val Ala Asp Thr Ala Arg Thr Gln Glu Arg 
        355                 360                 365             
Asp Leu Val Leu Gly Ser Phe Asn Arg Thr Gly Ile Ile Gly Val Ala 
    370                 375                 380                 
Val Asn Leu Tyr Arg Leu Ala Ile Glu Arg Gln Lys Pro Asp Ala Glu 
385                 390                 395                 400 
Arg Glu Pro Gly Tyr Gln Gln Arg Asp Leu Pro Val Ile Glu Gly Ser 
                405                 410                 415     
Leu Lys Gln Met Glu Arg Arg Tyr Val Pro Ala Met Asp Arg Gln Leu 
            420                 425                 430         
Arg Ala Tyr Trp Leu Asp Arg Tyr Val Ala Leu Pro Ala Ala Gln His 
        435                 440                 445             
Val Ala Ala Val Asp Ala Trp Leu Gly Gly Ser Asp Lys Ala Ala Ala 
    450                 455                 460                 
Glu Ala Ala Leu Ala Arg Leu Asp Gln Ser Arg Leu Gly Ser Leu Glu 
465                 470                 475                 480 
Glu Arg Leu Lys Trp Phe Asn Ala Asp Arg Ala Ala Phe Glu Ala Ser 
                485                 490                 495     
Thr Asp Pro Ala Ile Gln Tyr Ala Val Ala Val Met Pro Thr Leu Leu 
            500                 505                 510         
Ala Met Glu Gln Gln Ala Lys Thr Arg Tyr Gly Val Ala Leu Glu Ala 
        515                 520                 525             
Arg Pro Arg Tyr Leu Gln Ala Val Val Asp Tyr Lys Lys Ser Lys Gly 
    530                 535                 540                 
Gln Ala Val Tyr Pro Asp Ala Asn Ser Thr Leu Arg Ile Thr Tyr Gly 
545                 550                 555                 560 
His Val Lys Gly Tyr Thr Gly Leu Asn Gly Lys Val Tyr Thr Pro Phe 
                565                 570                 575     
Thr Thr Leu Glu Glu Val Ala Ala Lys Glu Thr Gly Val Glu Pro Phe 
            580                 585                 590         
Asp Asn Pro Lys Ala Leu Leu Glu Ala Val Ala Ala Lys Arg Tyr Ala 
        595                 600                 605             
Gly Leu Ala Asp Ala Arg Leu Gly Thr Val Pro Val Asn Phe Leu Ala 
    610                 615                 620                 
Asp Leu Asp Ile Thr Gly Gly Asn Ser Gly Ser Pro Val Leu Asp Ala 
625                 630                 635                 640 
Asn Gly Arg Leu Val Gly Leu Ala Phe Asp Gly Thr Leu Glu Ser Val 
                645                 650                 655     
Ala Ser Asn Trp Val Phe Asp Pro Val Leu Thr Arg Met Ile Ser Val 
            660                 665                 670         
Asp Gln Arg Tyr Met Arg Trp Ile Met Gln Glu Val Met Pro Ala Pro 
        675                 680                 685             
Gln Leu Leu Glu Glu Leu Gly Val Pro Pro Arg Gln 
    690                 695                 700 

<210> 5
<211> 710
<212> PRT
<213> Caldithrix abyssi


<220> 
<223> Dipeptidyl peptidase from Caldithrix abyssi (with the signal
      peptide)

<400> 5
Met Lys Ile Arg Leu Phe Gly Val Leu Leu Leu Phe Thr Phe Ser Leu 
1               5                   10                  15      
Phe Ala Glu Glu Gly Met Tyr Pro Ile Thr Glu Ile His Lys Leu Asn 
            20                  25                  30          
Leu Lys Lys Leu Gly Ile Glu Leu Ser Ala Asp Gln Ile Phe Ser Glu 
        35                  40                  45              
Asn Glu Val Ser Leu Ser Asp Ala Ile Val Gln Ile Gly Gly Cys Thr 
    50                  55                  60                  
Gly Ser Phe Ile Ser Pro Glu Gly Leu Ile Leu Thr Asn His His Cys 
65                  70                  75                  80  
Ala Phe Arg Ala Ile Gln Asn Ile Ser Ser Thr Glu Asn Asp Tyr Leu 
                85                  90                  95      
Thr Asn Gly Phe Val Ala His Thr Leu Gln Glu Glu Arg Pro Ala Lys 
            100                 105                 110         
Gly Tyr Thr Val Arg Ile Thr Glu Arg Val Glu Asp Val Ser Gln Arg 
        115                 120                 125             
Val Leu Asn Ala Val Lys His Ile Glu Asp Pro Ile Glu Arg Glu Lys 
    130                 135                 140                 
Ala Ile Glu Lys Ile Thr Lys Gln Ile Val Lys Glu Gln Glu Gln Lys 
145                 150                 155                 160 
His Pro Gly Lys Arg Ala Ala Val Ser Glu Met Phe Pro Gly Lys Thr 
                165                 170                 175     
Tyr Tyr Leu Phe Ile Tyr Thr Tyr Leu Lys Asp Val Arg Leu Val Tyr 
            180                 185                 190         
Ala Pro Pro Arg Ser Ile Gly Glu Phe Gly Gly Glu Phe Asp Asn Trp 
        195                 200                 205             
Glu Trp Pro Arg His Thr Gly Asp Phe Thr Leu Met Arg Ala Tyr Val 
    210                 215                 220                 
Ala Pro Asp Gly Ser Pro Ser Asp Tyr Ser Glu Glu Asn Val Pro Tyr 
225                 230                 235                 240 
Arg Pro Lys Ser Tyr Leu Lys Val Ala Ala Lys Gly Val Glu Glu Gly 
                245                 250                 255     
Asp Arg Val Phe Ile Leu Gly Tyr Pro Gly Arg Thr Tyr Arg His Arg 
            260                 265                 270         
Thr Ser Ala Phe Leu Ala Phe Glu Tyr Glu Phe Arg Met Pro Phe Val 
        275                 280                 285             
Val Asp Trp Tyr Gln Trp Gln Ile Asp Leu Leu Thr Thr Leu Gly Lys 
    290                 295                 300                 
Asp Asp Ala Asp Arg Ser Leu Lys Phe Ser Ser Trp Ile Lys Gly Leu 
305                 310                 315                 320 
Ala Asn Thr Glu Lys Asn Tyr Arg Gly Lys Leu Gln Gly Ile Arg Arg 
                325                 330                 335     
Ile Gly Leu Leu Glu Gln Lys Lys Asn Glu Glu Glu Lys Ile Gln Val 
            340                 345                 350         
Phe Ile Ala Glu Asn Asn Leu Lys Lys Tyr Gln His Val Leu Thr Glu 
        355                 360                 365             
Ile Lys Gln Ile Tyr His Thr Tyr Arg Gln Ser Ala Val Arg Glu Met 
    370                 375                 380                 
Leu Leu Ser Tyr Phe Gly Arg Ser Pro Val Leu Pro Ala Val Ala Arg 
385                 390                 395                 400 
Thr Leu Val Leu Ala Ala Glu Glu Arg Gln Lys Glu Asp Leu Glu Arg 
                405                 410                 415     
Glu Arg Ala Phe Met Asp Arg Asn Phe Lys Arg Thr Gln Thr Tyr Thr 
            420                 425                 430         
Leu Leu Arg Leu Lys Asn Phe Asp Ser Gln Ala Asp Gln Leu Ile Leu 
        435                 440                 445             
Gln Glu Leu Leu Lys Lys Ala Ala Ala Leu Pro Glu Asp Gln Arg Ile 
    450                 455                 460                 
Ser Ala Leu Arg Ser Ile Phe Lys Leu Asp Asp Ala Ala Glu Thr Arg 
465                 470                 475                 480 
Gln Val Ile Ser Glu Ala Tyr Arg Lys Thr Arg Leu Ser Asp Pro Glu 
                485                 490                 495     
Phe Val Lys Thr Cys Phe Ala Lys Thr Pro Asp Glu Leu Lys Ala Leu 
            500                 505                 510         
Asn Asp Pro Leu Ile Asn Trp Met Leu Ala Leu Lys Glu Asp Tyr Glu 
        515                 520                 525             
Thr Leu Lys Asn Ile Arg Lys Glu Arg Asn Gly Lys Leu Arg Arg Leu 
    530                 535                 540                 
Arg Ala Leu Trp Leu Glu Ala Lys Gln Ala Tyr Leu Lys Thr Asp Phe 
545                 550                 555                 560 
Ile Pro Asp Ala Asn Gly Thr Tyr Arg Met Thr Phe Gly Phe Ile Glu 
                565                 570                 575     
Gly Tyr Ala Pro Ala Asp Ala Val Tyr Lys Ala Pro Ile Thr Thr Gly 
            580                 585                 590         
Arg Gly Ile Leu Glu Lys His Thr Gly Lys Ser Pro Phe Asp Thr Pro 
        595                 600                 605             
Glu Lys Leu Leu Ala Leu Leu Lys Ala Lys Gln Phe Gly Pro Phe Val 
    610                 615                 620                 
Ser Lys Thr Val Gly Thr Leu Pro Val Gly Ile Leu Tyr Ser Cys Asp 
625                 630                 635                 640 
Thr Thr Gly Gly Asn Ser Gly Ser Pro Val Leu Asn Ala Arg Gly Gln 
                645                 650                 655     
Leu Val Gly Leu Asn Phe Asp Arg Ala Phe Glu Ala Thr Ile Asn Asp 
            660                 665                 670         
Tyr Ala Trp Asn His Gln Tyr Ser Arg Ser Ile Gly Val Asp Ile Arg 
        675                 680                 685             
Tyr Ile Leu Phe Leu Leu Lys Tyr Phe Ser Gly Ala Glu His Leu Leu 
    690                 695                 700                 
Glu Glu Met Gly Val Gln 
705                 710 

<210> 6
<211> 692
<212> PRT
<213> Caldithrix abyssi


<220> 
<223> Dipeptidyl peptidase from Caldithrix abyssi (without the signal
      peptide)

<400> 6
Glu Glu Gly Met Tyr Pro Ile Thr Glu Ile His Lys Leu Asn Leu Lys 
1               5                   10                  15      
Lys Leu Gly Ile Glu Leu Ser Ala Asp Gln Ile Phe Ser Glu Asn Glu 
            20                  25                  30          
Val Ser Leu Ser Asp Ala Ile Val Gln Ile Gly Gly Cys Thr Gly Ser 
        35                  40                  45              
Phe Ile Ser Pro Glu Gly Leu Ile Leu Thr Asn His His Cys Ala Phe 
    50                  55                  60                  
Arg Ala Ile Gln Asn Ile Ser Ser Thr Glu Asn Asp Tyr Leu Thr Asn 
65                  70                  75                  80  
Gly Phe Val Ala His Thr Leu Gln Glu Glu Arg Pro Ala Lys Gly Tyr 
                85                  90                  95      
Thr Val Arg Ile Thr Glu Arg Val Glu Asp Val Ser Gln Arg Val Leu 
            100                 105                 110         
Asn Ala Val Lys His Ile Glu Asp Pro Ile Glu Arg Glu Lys Ala Ile 
        115                 120                 125             
Glu Lys Ile Thr Lys Gln Ile Val Lys Glu Gln Glu Gln Lys His Pro 
    130                 135                 140                 
Gly Lys Arg Ala Ala Val Ser Glu Met Phe Pro Gly Lys Thr Tyr Tyr 
145                 150                 155                 160 
Leu Phe Ile Tyr Thr Tyr Leu Lys Asp Val Arg Leu Val Tyr Ala Pro 
                165                 170                 175     
Pro Arg Ser Ile Gly Glu Phe Gly Gly Glu Phe Asp Asn Trp Glu Trp 
            180                 185                 190         
Pro Arg His Thr Gly Asp Phe Thr Leu Met Arg Ala Tyr Val Ala Pro 
        195                 200                 205             
Asp Gly Ser Pro Ser Asp Tyr Ser Glu Glu Asn Val Pro Tyr Arg Pro 
    210                 215                 220                 
Lys Ser Tyr Leu Lys Val Ala Ala Lys Gly Val Glu Glu Gly Asp Arg 
225                 230                 235                 240 
Val Phe Ile Leu Gly Tyr Pro Gly Arg Thr Tyr Arg His Arg Thr Ser 
                245                 250                 255     
Ala Phe Leu Ala Phe Glu Tyr Glu Phe Arg Met Pro Phe Val Val Asp 
            260                 265                 270         
Trp Tyr Gln Trp Gln Ile Asp Leu Leu Thr Thr Leu Gly Lys Asp Asp 
        275                 280                 285             
Ala Asp Arg Ser Leu Lys Phe Ser Ser Trp Ile Lys Gly Leu Ala Asn 
    290                 295                 300                 
Thr Glu Lys Asn Tyr Arg Gly Lys Leu Gln Gly Ile Arg Arg Ile Gly 
305                 310                 315                 320 
Leu Leu Glu Gln Lys Lys Asn Glu Glu Glu Lys Ile Gln Val Phe Ile 
                325                 330                 335     
Ala Glu Asn Asn Leu Lys Lys Tyr Gln His Val Leu Thr Glu Ile Lys 
            340                 345                 350         
Gln Ile Tyr His Thr Tyr Arg Gln Ser Ala Val Arg Glu Met Leu Leu 
        355                 360                 365             
Ser Tyr Phe Gly Arg Ser Pro Val Leu Pro Ala Val Ala Arg Thr Leu 
    370                 375                 380                 
Val Leu Ala Ala Glu Glu Arg Gln Lys Glu Asp Leu Glu Arg Glu Arg 
385                 390                 395                 400 
Ala Phe Met Asp Arg Asn Phe Lys Arg Thr Gln Thr Tyr Thr Leu Leu 
                405                 410                 415     
Arg Leu Lys Asn Phe Asp Ser Gln Ala Asp Gln Leu Ile Leu Gln Glu 
            420                 425                 430         
Leu Leu Lys Lys Ala Ala Ala Leu Pro Glu Asp Gln Arg Ile Ser Ala 
        435                 440                 445             
Leu Arg Ser Ile Phe Lys Leu Asp Asp Ala Ala Glu Thr Arg Gln Val 
    450                 455                 460                 
Ile Ser Glu Ala Tyr Arg Lys Thr Arg Leu Ser Asp Pro Glu Phe Val 
465                 470                 475                 480 
Lys Thr Cys Phe Ala Lys Thr Pro Asp Glu Leu Lys Ala Leu Asn Asp 
                485                 490                 495     
Pro Leu Ile Asn Trp Met Leu Ala Leu Lys Glu Asp Tyr Glu Thr Leu 
            500                 505                 510         
Lys Asn Ile Arg Lys Glu Arg Asn Gly Lys Leu Arg Arg Leu Arg Ala 
        515                 520                 525             
Leu Trp Leu Glu Ala Lys Gln Ala Tyr Leu Lys Thr Asp Phe Ile Pro 
    530                 535                 540                 
Asp Ala Asn Gly Thr Tyr Arg Met Thr Phe Gly Phe Ile Glu Gly Tyr 
545                 550                 555                 560 
Ala Pro Ala Asp Ala Val Tyr Lys Ala Pro Ile Thr Thr Gly Arg Gly 
                565                 570                 575     
Ile Leu Glu Lys His Thr Gly Lys Ser Pro Phe Asp Thr Pro Glu Lys 
            580                 585                 590         
Leu Leu Ala Leu Leu Lys Ala Lys Gln Phe Gly Pro Phe Val Ser Lys 
        595                 600                 605             
Thr Val Gly Thr Leu Pro Val Gly Ile Leu Tyr Ser Cys Asp Thr Thr 
    610                 615                 620                 
Gly Gly Asn Ser Gly Ser Pro Val Leu Asn Ala Arg Gly Gln Leu Val 
625                 630                 635                 640 
Gly Leu Asn Phe Asp Arg Ala Phe Glu Ala Thr Ile Asn Asp Tyr Ala 
                645                 650                 655     
Trp Asn His Gln Tyr Ser Arg Ser Ile Gly Val Asp Ile Arg Tyr Ile 
            660                 665                 670         
Leu Phe Leu Leu Lys Tyr Phe Ser Gly Ala Glu His Leu Leu Glu Glu 
        675                 680                 685             
Met Gly Val Gln 
    690         

<210> 7
<211> 256
<212> PRT
<213> Homo sapiens


<220> 
<223> Carbonic anhydrase II scaffold

<400> 7
His Trp Gly Tyr Gly Lys His Asn Gly Pro Glu His Trp His Lys Asp 
1               5                   10                  15      
Phe Pro Ile Ala Lys Gly Glu Arg Gln Ser Pro Val Asp Ile Asp Thr 
            20                  25                  30          
His Thr Ala Lys Tyr Asp Pro Ser Leu Lys Pro Leu Ser Val Ser Tyr 
        35                  40                  45              
Asp Gln Ala Thr Ser Leu Arg Ile Leu Asn Asn Gly His Ala Phe Asn 
    50                  55                  60                  
Val Glu Phe Asp Asp Ser Gln Asp Lys Ala Val Leu Lys Gly Gly Pro 
65                  70                  75                  80  
Leu Asp Gly Thr Tyr Arg Leu Ile Gln Phe His Phe His Trp Gly Ser 
                85                  90                  95      
Leu Asp Gly Gln Gly Ser Glu His Thr Val Asp Lys Lys Lys Tyr Ala 
            100                 105                 110         
Ala Glu Leu His Leu Val His Trp Asn Thr Lys Tyr Gly Asp Phe Gly 
        115                 120                 125             
Lys Ala Val Gln Gln Pro Asp Gly Leu Ala Val Leu Gly Ile Phe Leu 
    130                 135                 140                 
Lys Val Gly Ser Ala Lys Pro Gly Leu Gln Lys Val Val Asp Val Leu 
145                 150                 155                 160 
Asp Ser Ile Lys Thr Lys Gly Lys Ser Ala Asp Phe Thr Asn Phe Asp 
                165                 170                 175     
Pro Arg Gly Leu Leu Pro Glu Ser Leu Asp Tyr Trp Thr Tyr Pro Gly 
            180                 185                 190         
Ser Leu Thr Thr Pro Pro Leu Leu Glu Cys Val Thr Trp Ile Val Leu 
        195                 200                 205             
Lys Glu Pro Ile Ser Val Ser Ser Glu Gln Val Leu Lys Phe Arg Lys 
    210                 215                 220                 
Leu Asn Phe Asn Gly Glu Gly Glu Pro Glu Glu Leu Met Val Asp Asn 
225                 230                 235                 240 
Trp Arg Pro Ala Gln Pro Leu Lys Asn Arg Gln Ile Lys Ala Ser Phe 
                245                 250                 255     



<210> 8
<211> 256
<212> PRT
<213> Homo sapiens


<220> 
<223> Carbonic anhydrase I scaffold

<400> 8
Trp Gly Tyr Asp Asp Lys Asn Gly Pro Glu Gln Trp Ser Lys Leu Tyr 
1               5                   10                  15      
Pro Ile Ala Asn Gly Asn Asn Gln Ser Pro Val Asp Ile Lys Thr Ser 
            20                  25                  30          
Glu Thr Lys His Asp Thr Ser Leu Lys Pro Ile Ser Val Ser Tyr Asn 
        35                  40                  45              
Pro Ala Thr Ala Lys Glu Ile Ile Asn Val Gly His Ser Phe His Val 
    50                  55                  60                  
Asn Phe Glu Asp Asn Gln Asp Arg Ser Val Leu Lys Gly Gly Pro Phe 
65                  70                  75                  80  
Ser Asp Ser Tyr Arg Leu Phe Gln Phe His Phe His Trp Gly Ser Thr 
                85                  90                  95      
Asn Glu His Gly Ser Glu His Thr Val Asp Gly Val Lys Tyr Ser Ala 
            100                 105                 110         
Glu Leu His Val Ala His Trp Asn Ser Ala Lys Tyr Ser Ser Leu Ala 
        115                 120                 125             
Glu Ala Ala Ser Lys Ala Asp Gly Leu Ala Val Ile Gly Val Leu Met 
    130                 135                 140                 
Lys Val Gly Glu Ala Asn Pro Lys Leu Gln Lys Val Leu Asp Ala Leu 
145                 150                 155                 160 
Gln Ala Ile Lys Thr Lys Gly Lys Arg Ala Pro Phe Thr Asn Phe Asp 
                165                 170                 175     
Pro Ser Thr Leu Leu Pro Ser Ser Leu Asp Phe Trp Thr Tyr Pro Gly 
            180                 185                 190         
Ser Leu Thr His Pro Pro Leu Tyr Glu Ser Val Thr Trp Ile Ile Cys 
        195                 200                 205             
Lys Glu Ser Ile Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
    210                 215                 220                 
Leu Leu Ser Asn Val Glu Gly Asp Asn Ala Val Pro Met Gln His Asn 
225                 230                 235                 240 
Asn Arg Pro Thr Gln Pro Leu Lys Gly Arg Thr Val Arg Ala Ser Phe 
                245                 250                 255     



<210> 9
<211> 256
<212> PRT
<213> Homo sapiens


<220> 
<223> Carbonic anhydrase III scaffold

<400> 9
Glu Trp Gly Tyr Ala Ser His Asn Gly Pro Asp His Trp His Glu Leu 
1               5                   10                  15      
Phe Pro Asn Ala Lys Gly Glu Asn Gln Ser Pro Ile Glu Leu His Thr 
            20                  25                  30          
Lys Asp Ile Arg His Asp Pro Ser Leu Gln Pro Trp Ser Val Ser Tyr 
        35                  40                  45              
Asp Gly Gly Ser Ala Lys Thr Ile Leu Asn Asn Gly Lys Thr Cys Arg 
    50                  55                  60                  
Val Val Phe Asp Asp Thr Tyr Asp Arg Ser Met Leu Arg Gly Gly Pro 
65                  70                  75                  80  
Leu Pro Gly Pro Tyr Arg Leu Arg Gln Phe His Leu His Trp Gly Ser 
                85                  90                  95      
Ser Asp Asp His Gly Ser Glu His Thr Val Asp Gly Val Lys Tyr Ala 
            100                 105                 110         
Ala Glu Leu His Leu Val His Trp Asn Pro Lys Tyr Asn Thr Phe Lys 
        115                 120                 125             
Glu Ala Leu Lys Gln Arg Asp Gly Ile Ala Val Ile Gly Ile Phe Leu 
    130                 135                 140                 
Lys Ile Gly His Glu Asn Gly Glu Phe Gln Ile Phe Leu Asp Ala Leu 
145                 150                 155                 160 
Asp Lys Ile Lys Thr Lys Gly Lys Glu Ala Pro Phe Thr Lys Phe Asp 
                165                 170                 175     
Pro Ser Ser Leu Phe Pro Ala Ser Arg Asp Tyr Trp Thr Tyr Gln Gly 
            180                 185                 190         
Ser Leu Thr Thr Pro Pro Cys Glu Glu Cys Ile Val Trp Leu Leu Leu 
        195                 200                 205             
Lys Glu Pro Met Thr Val Ser Ser Asp Gln Met Ala Lys Leu Arg Ser 
    210                 215                 220                 
Leu Leu Ser Ser Ala Glu Asn Glu Pro Pro Val Pro Leu Val Ser Asn 
225                 230                 235                 240 
Trp Arg Pro Pro Gln Pro Ile Asn Asn Arg Val Val Arg Ala Ser Phe 
                245                 250                 255     



<210> 10
<211> 266
<212> PRT
<213> Homo sapiens


<220> 
<223> Carbonic anhydrase IV scaffold

<400> 10
Ala Glu Ser His Trp Cys Tyr Glu Val Gln Ala Glu Ser Ser Asn Tyr 
1               5                   10                  15      
Pro Cys Leu Val Pro Val Lys Trp Gly Gly Asn Cys Gln Lys Asp Arg 
            20                  25                  30          
Gln Ser Pro Ile Asn Ile Val Thr Thr Lys Ala Lys Val Asp Lys Lys 
        35                  40                  45              
Leu Gly Arg Phe Phe Phe Ser Gly Tyr Asp Lys Lys Gln Thr Trp Thr 
    50                  55                  60                  
Val Gln Asn Asn Gly His Ser Val Met Met Leu Leu Glu Asn Lys Ala 
65                  70                  75                  80  
Ser Ile Ser Gly Gly Gly Leu Pro Ala Pro Tyr Gln Ala Lys Gln Leu 
                85                  90                  95      
His Leu His Trp Ser Asp Leu Pro Tyr Lys Gly Ser Glu His Ser Leu 
            100                 105                 110         
Asp Gly Glu His Phe Ala Met Glu Met His Ile Val His Glu Lys Glu 
        115                 120                 125             
Lys Gly Thr Ser Arg Asn Val Lys Glu Ala Gln Asp Pro Glu Asp Glu 
    130                 135                 140                 
Ile Ala Val Leu Ala Phe Leu Val Glu Ala Gly Thr Gln Val Asn Glu 
145                 150                 155                 160 
Gly Phe Gln Pro Leu Val Glu Ala Leu Ser Asn Ile Pro Lys Pro Glu 
                165                 170                 175     
Met Ser Thr Thr Met Ala Glu Ser Ser Leu Leu Asp Leu Leu Pro Lys 
            180                 185                 190         
Glu Glu Lys Leu Arg His Tyr Phe Arg Tyr Leu Gly Ser Leu Thr Thr 
        195                 200                 205             
Pro Thr Cys Asp Glu Lys Val Val Trp Thr Val Phe Arg Glu Pro Ile 
    210                 215                 220                 
Gln Leu His Arg Glu Gln Ile Leu Ala Phe Ser Gln Lys Leu Tyr Tyr 
225                 230                 235                 240 
Asp Lys Glu Gln Thr Val Ser Met Lys Asp Asn Val Arg Pro Leu Gln 
                245                 250                 255     
Gln Leu Gly Gln Arg Thr Val Ile Lys Ser 
            260                 265     

<210> 11
<211> 262
<212> PRT
<213> Homo sapiens


<220> 
<223> Carbonic anhydrase VII scaffold

<400> 11
Gly His His Gly Trp Gly Tyr Gly Gln Asp Asp Gly Pro Ser His Trp 
1               5                   10                  15      
His Lys Leu Tyr Pro Ile Ala Gln Gly Asp Arg Gln Ser Pro Ile Asn 
            20                  25                  30          
Ile Ile Ser Ser Gln Ala Val Tyr Ser Pro Ser Leu Gln Pro Leu Glu 
        35                  40                  45              
Leu Ser Tyr Glu Ala Cys Met Ser Leu Ser Ile Thr Asn Asn Gly His 
    50                  55                  60                  
Ser Val Gln Val Asp Phe Asn Asp Ser Asp Asp Arg Thr Val Val Thr 
65                  70                  75                  80  
Gly Gly Pro Leu Glu Gly Pro Tyr Arg Leu Lys Gln Phe His Phe His 
                85                  90                  95      
Trp Gly Lys Lys His Asp Val Gly Ser Glu His Thr Val Asp Gly Lys 
            100                 105                 110         
Ser Phe Pro Ser Glu Leu His Leu Val His Trp Asn Ala Lys Lys Tyr 
        115                 120                 125             
Ser Thr Phe Gly Glu Ala Ala Ser Ala Pro Asp Gly Leu Ala Val Val 
    130                 135                 140                 
Gly Val Phe Leu Glu Thr Gly Asp Glu His Pro Ser Met Asn Arg Leu 
145                 150                 155                 160 
Thr Asp Ala Leu Tyr Met Val Arg Phe Lys Gly Thr Lys Ala Gln Phe 
                165                 170                 175     
Ser Cys Phe Asn Pro Lys Ser Leu Leu Pro Ala Ser Arg His Tyr Trp 
            180                 185                 190         
Thr Tyr Pro Gly Ser Leu Thr Thr Pro Pro Leu Ser Glu Ser Val Thr 
        195                 200                 205             
Trp Ile Val Leu Arg Glu Pro Ile Ser Ile Ser Glu Arg Gln Met Gly 
    210                 215                 220                 
Lys Phe Arg Ser Leu Leu Phe Thr Ser Glu Asp Asp Glu Arg Ile His 
225                 230                 235                 240 
Met Val Asn Asn Phe Arg Pro Pro Gln Pro Leu Lys Gly Arg Val Val 
                245                 250                 255     
Lys Ala Ser Phe Arg Ala 
            260         

<210> 12
<211> 260
<212> PRT
<213> Homo sapiens


<220> 
<223> Carbonic anhydrase XII scaffold

<400> 12
Lys Trp Thr Tyr Phe Gly Pro Asp Gly Glu Asn Ser Trp Ser Lys Lys 
1               5                   10                  15      
Tyr Pro Ser Cys Gly Gly Leu Leu Gln Ser Pro Ile Asp Leu His Ser 
            20                  25                  30          
Asp Ile Leu Gln Tyr Asp Ala Ser Leu Thr Pro Leu Glu Phe Gln Gly 
        35                  40                  45              
Tyr Asn Leu Ser Ala Asn Lys Gln Phe Leu Leu Thr Asn Asn Gly His 
    50                  55                  60                  
Ser Val Lys Leu Asn Leu Pro Ser Asp Met His Ile Gln Gly Leu Gln 
65                  70                  75                  80  
Ser Arg Tyr Ser Ala Thr Gln Leu His Leu His Trp Gly Asn Pro Asn 
                85                  90                  95      
Asp Pro His Gly Ser Glu His Thr Val Ser Gly Gln His Phe Ala Ala 
            100                 105                 110         
Glu Leu His Ile Val His Tyr Asn Ser Asp Leu Tyr Pro Asp Ala Ser 
        115                 120                 125             
Thr Ala Ser Asn Lys Ser Glu Gly Leu Ala Val Leu Ala Val Leu Ile 
    130                 135                 140                 
Glu Met Gly Ser Phe Asn Pro Ser Tyr Asp Lys Ile Phe Ser His Leu 
145                 150                 155                 160 
Gln His Val Lys Tyr Lys Gly Gln Glu Ala Phe Val Pro Gly Phe Asn 
                165                 170                 175     
Ile Glu Glu Leu Leu Pro Glu Arg Thr Ala Glu Tyr Tyr Arg Tyr Arg 
            180                 185                 190         
Gly Ser Leu Thr Thr Pro Pro Cys Asn Pro Thr Val Leu Trp Thr Val 
        195                 200                 205             
Phe Arg Asn Pro Val Gln Ile Ser Gln Glu Gln Leu Leu Ala Leu Glu 
    210                 215                 220                 
Thr Ala Leu Tyr Cys Thr His Met Asp Asp Pro Ser Pro Arg Glu Met 
225                 230                 235                 240 
Ile Asn Asn Phe Arg Gln Val Gln Lys Phe Asp Glu Arg Leu Val Tyr 
                245                 250                 255     
Thr Ser Phe Ser 
            260 

<210> 13
<211> 259
<212> PRT
<213> Homo sapiens


<220> 
<223> Carbonic anhydrase XIII scaffold

<400> 13
Leu Ser Trp Gly Tyr Arg Glu His Asn Gly Pro Ile His Trp Lys Glu 
1               5                   10                  15      
Phe Phe Pro Ile Ala Asp Gly Asp Gln Gln Ser Pro Ile Glu Ile Lys 
            20                  25                  30          
Thr Lys Glu Val Lys Tyr Asp Ser Ser Leu Arg Pro Leu Ser Ile Lys 
        35                  40                  45              
Tyr Asp Pro Ser Ser Ala Lys Ile Ile Ser Asn Ser Gly His Ser Phe 
    50                  55                  60                  
Asn Val Asp Phe Asp Asp Thr Glu Asn Lys Ser Val Leu Arg Gly Gly 
65                  70                  75                  80  
Pro Leu Thr Gly Ser Tyr Arg Leu Arg Gln Val His Leu His Trp Gly 
                85                  90                  95      
Ser Ala Asp Asp His Gly Ser Glu His Ile Val Asp Gly Val Ser Tyr 
            100                 105                 110         
Ala Ala Glu Leu His Val Val His Trp Asn Ser Asp Lys Tyr Pro Ser 
        115                 120                 125             
Phe Val Glu Ala Ala His Glu Pro Asp Gly Leu Ala Val Leu Gly Val 
    130                 135                 140                 
Phe Leu Gln Ile Gly Glu Pro Asn Ser Gln Leu Gln Lys Ile Thr Asp 
145                 150                 155                 160 
Thr Leu Asp Ser Ile Lys Glu Lys Gly Lys Gln Thr Arg Phe Thr Asn 
                165                 170                 175     
Phe Asp Leu Leu Ser Leu Leu Pro Pro Ser Trp Asp Tyr Trp Thr Tyr 
            180                 185                 190         
Pro Gly Ser Leu Thr Val Pro Pro Leu Leu Glu Ser Val Thr Trp Ile 
        195                 200                 205             
Val Leu Lys Gln Pro Ile Asn Ile Ser Ser Gln Gln Leu Ala Lys Phe 
    210                 215                 220                 
Arg Ser Leu Leu Cys Thr Ala Glu Gly Glu Ala Ala Ala Phe Leu Val 
225                 230                 235                 240 
Ser Asn His Arg Pro Pro Gln Pro Leu Lys Gly Arg Lys Val Arg Ala 
                245                 250                 255     
Ser Phe His 
            

<210> 14
<211> 261
<212> PRT
<213> Homo sapiens


<220> 
<223> ADAM17 (aka TACE) scaffold

<400> 14
Ala Asp Pro Asp Pro Met Lys Asn Thr Cys Lys Leu Leu Val Val Ala 
1               5                   10                  15      
Asp His Arg Phe Tyr Arg Tyr Met Gly Arg Gly Glu Glu Ser Thr Thr 
            20                  25                  30          
Thr Asn Tyr Leu Ile Glu Leu Ile Asp Arg Val Asp Asp Ile Tyr Arg 
        35                  40                  45              
Asn Thr Ala Trp Asp Asn Ala Gly Phe Lys Gly Tyr Gly Ile Gln Ile 
    50                  55                  60                  
Glu Gln Ile Arg Ile Leu Lys Ser Pro Gln Glu Val Lys Pro Gly Glu 
65                  70                  75                  80  
Lys His Tyr Asn Met Ala Lys Ser Tyr Pro Asn Glu Glu Lys Asp Ala 
                85                  90                  95      
Trp Asp Val Lys Met Leu Leu Glu Gln Phe Ser Phe Asp Ile Ala Glu 
            100                 105                 110         
Glu Ala Ser Lys Val Cys Leu Ala His Leu Phe Thr Tyr Gln Asp Phe 
        115                 120                 125             
Asp Met Gly Thr Leu Gly Leu Ala Tyr Gly Gly Ser Pro Arg Ala Asn 
    130                 135                 140                 
Ser His Gly Gly Val Cys Pro Lys Ala Tyr Tyr Ser Pro Val Gly Lys 
145                 150                 155                 160 
Lys Asn Ile Tyr Leu Asn Ser Gly Leu Thr Ser Thr Lys Asn Tyr Gly 
                165                 170                 175     
Lys Thr Ile Leu Thr Lys Glu Ala Asp Leu Val Thr Thr His Glu Leu 
            180                 185                 190         
Gly His Asn Phe Gly Ala Glu His Asp Pro Asp Gly Leu Ala Glu Cys 
        195                 200                 205             
Ala Pro Asn Glu Asp Gln Gly Gly Lys Tyr Val Met Tyr Pro Ile Ala 
    210                 215                 220                 
Val Ser Gly Asp His Glu Asn Asn Lys Met Phe Ser Gln Cys Ser Lys 
225                 230                 235                 240 
Gln Ser Ile Tyr Lys Thr Ile Glu Ser Lys Ala Gln Glu Cys Phe Gln 
                245                 250                 255     
Glu Arg Ser Asn Ala 
            260     

<210> 15
<211> 200
<212> PRT
<213> Astacus astacus


<220> 
<223> Astacin, scaffold

<400> 15
Ala Ala Ile Leu Gly Asp Glu Tyr Leu Trp Ser Gly Gly Val Ile Pro 
1               5                   10                  15      
Tyr Thr Phe Ala Gly Val Ser Gly Ala Asp Gln Ser Ala Ile Leu Ser 
            20                  25                  30          
Gly Met Gln Glu Leu Glu Glu Lys Thr Cys Ile Arg Phe Val Pro Arg 
        35                  40                  45              
Thr Thr Glu Ser Asp Tyr Val Glu Ile Phe Thr Ser Gly Ser Gly Cys 
    50                  55                  60                  
Trp Ser Tyr Val Gly Arg Ile Ser Gly Ala Gln Gln Val Ser Leu Gln 
65                  70                  75                  80  
Ala Asn Gly Cys Val Tyr His Gly Thr Ile Ile His Glu Leu Met His 
                85                  90                  95      
Ala Ile Gly Phe Tyr His Glu His Thr Arg Met Asp Arg Asp Asn Tyr 
            100                 105                 110         
Val Thr Ile Asn Tyr Gln Asn Val Asp Pro Ser Met Thr Ser Asn Phe 
        115                 120                 125             
Asp Ile Asp Thr Tyr Ser Arg Tyr Val Gly Glu Asp Tyr Gln Tyr Tyr 
    130                 135                 140                 
Ser Ile Met His Tyr Gly Lys Tyr Ser Phe Ser Ile Gln Trp Gly Val 
145                 150                 155                 160 
Leu Glu Thr Ile Val Pro Leu Gln Asn Gly Ile Asp Leu Thr Asp Pro 
                165                 170                 175     
Tyr Asp Lys Ala His Met Leu Gln Thr Asp Ala Asn Gln Ile Asn Asn 
            180                 185                 190         
Leu Tyr Thr Asn Glu Cys Ser Leu 
        195                 200 

<210> 16
<211> 866
<212> PRT
<213> Escherichia coli


<220> 
<223> Aminopeptidase N, scaffold

<400> 16
Pro Gln Ala Lys Tyr Arg His Asp Tyr Arg Ala Pro Asp Tyr Gln Ile 
1               5                   10                  15      
Thr Asp Ile Asp Leu Thr Phe Asp Leu Asp Ala Gln Lys Thr Val Val 
            20                  25                  30          
Thr Ala Val Ser Gln Ala Val Arg His Gly Ala Ser Asp Ala Pro Leu 
        35                  40                  45              
Arg Leu Asn Gly Glu Asp Leu Lys Leu Val Ser Val His Ile Asn Asp 
    50                  55                  60                  
Glu Pro Trp Thr Ala Trp Lys Glu Glu Glu Gly Ala Leu Val Ile Ser 
65                  70                  75                  80  
Asn Leu Pro Glu Arg Phe Thr Leu Lys Ile Ile Asn Glu Ile Ser Pro 
                85                  90                  95      
Ala Ala Asn Thr Ala Leu Glu Gly Leu Tyr Gln Ser Gly Asp Ala Leu 
            100                 105                 110         
Cys Thr Gln Cys Glu Ala Glu Gly Phe Arg His Ile Thr Tyr Tyr Leu 
        115                 120                 125             
Asp Arg Pro Asp Val Leu Ala Arg Phe Thr Thr Lys Ile Ile Ala Asp 
    130                 135                 140                 
Lys Ile Lys Tyr Pro Phe Leu Leu Ser Asn Gly Asn Arg Val Ala Gln 
145                 150                 155                 160 
Gly Glu Leu Glu Asn Gly Arg His Trp Val Gln Trp Gln Asp Pro Phe 
                165                 170                 175     
Pro Lys Pro Cys Tyr Leu Phe Ala Leu Val Ala Gly Asp Phe Asp Val 
            180                 185                 190         
Leu Arg Asp Thr Phe Thr Thr Arg Ser Gly Arg Glu Val Ala Leu Glu 
        195                 200                 205             
Leu Tyr Val Asp Arg Gly Asn Leu Asp Arg Ala Pro Trp Ala Met Thr 
    210                 215                 220                 
Ser Leu Lys Asn Ser Met Lys Trp Asp Glu Glu Arg Phe Gly Leu Glu 
225                 230                 235                 240 
Tyr Asp Leu Asp Ile Tyr Met Ile Val Ala Val Asp Phe Phe Asn Met 
                245                 250                 255     
Gly Ala Met Glu Asn Lys Gly Leu Asn Ile Phe Asn Ser Lys Tyr Val 
            260                 265                 270         
Leu Ala Arg Thr Asp Thr Ala Thr Asp Lys Asp Tyr Leu Asp Ile Glu 
        275                 280                 285             
Arg Val Ile Gly His Glu Tyr Phe His Asn Trp Thr Gly Asn Arg Val 
    290                 295                 300                 
Thr Cys Arg Asp Trp Phe Gln Leu Ser Leu Lys Glu Gly Leu Thr Val 
305                 310                 315                 320 
Phe Arg Asp Gln Glu Phe Ser Ser Asp Leu Gly Ser Arg Ala Val Asn 
                325                 330                 335     
Arg Ile Asn Asn Val Arg Thr Met Arg Gly Leu Gln Phe Ala Glu Asp 
            340                 345                 350         
Ala Ser Pro Met Ala His Pro Ile Arg Pro Asp Met Val Ile Glu Met 
        355                 360                 365             
Asn Asn Phe Tyr Thr Leu Thr Val Tyr Glu Lys Gly Ala Glu Val Ile 
    370                 375                 380                 
Arg Met Ile His Thr Leu Leu Gly Glu Glu Asn Phe Gln Lys Gly Met 
385                 390                 395                 400 
Gln Leu Tyr Phe Glu Arg His Asp Gly Ser Ala Ala Thr Cys Asp Asp 
                405                 410                 415     
Phe Val Gln Ala Met Glu Asp Ala Ser Asn Val Asp Leu Ser His Phe 
            420                 425                 430         
Arg Arg Trp Tyr Ser Gln Ser Gly Thr Pro Ile Val Thr Val Lys Asp 
        435                 440                 445             
Asp Tyr Asn Pro Glu Thr Glu Gln Tyr Thr Leu Thr Ile Ser Gln Arg 
    450                 455                 460                 
Thr Pro Ala Thr Pro Asp Gln Ala Glu Lys Gln Pro Leu His Ile Pro 
465                 470                 475                 480 
Phe Ala Ile Glu Leu Tyr Asp Asn Glu Gly Lys Val Ile Pro Leu Gln 
                485                 490                 495     
Lys Gly Gly His Pro Val Asn Ser Val Leu Asn Val Thr Gln Ala Glu 
            500                 505                 510         
Gln Thr Phe Val Phe Asp Asn Val Tyr Phe Gln Pro Val Pro Ala Leu 
        515                 520                 525             
Leu Cys Glu Phe Ser Ala Pro Val Lys Leu Glu Tyr Lys Trp Ser Asp 
    530                 535                 540                 
Gln Gln Leu Thr Phe Leu Met Arg His Ala Arg Asn Asp Phe Ser Arg 
545                 550                 555                 560 
Trp Asp Ala Ala Gln Ser Leu Leu Ala Thr Tyr Ile Lys Leu Asn Val 
                565                 570                 575     
Ala Arg His Gln Gln Gly Gln Pro Leu Ser Leu Pro Val His Val Ala 
            580                 585                 590         
Asp Ala Phe Arg Ala Val Leu Leu Asp Glu Lys Ile Asp Pro Ala Leu 
        595                 600                 605             
Ala Ala Glu Ile Leu Thr Leu Pro Ser Val Asn Glu Met Ala Glu Leu 
    610                 615                 620                 
Phe Asp Ile Ile Asp Pro Ile Ala Ile Ala Glu Val Arg Glu Ala Leu 
625                 630                 635                 640 
Thr Arg Thr Leu Ala Thr Glu Leu Ala Asp Glu Leu Leu Ala Ile Tyr 
                645                 650                 655     
Asn Ala Asn Tyr Gln Ser Glu Tyr Arg Val Glu His Glu Asp Ile Ala 
            660                 665                 670         
Lys Arg Thr Leu Arg Asn Ala Cys Leu Arg Phe Leu Ala Phe Gly Glu 
        675                 680                 685             
Thr His Leu Ala Asp Val Leu Val Ser Lys Gln Phe His Glu Ala Asn 
    690                 695                 700                 
Asn Met Thr Asp Ala Leu Ala Ala Leu Ser Ala Ala Val Ala Ala Gln 
705                 710                 715                 720 
Leu Pro Cys Arg Asp Ala Leu Met Gln Glu Tyr Asp Asp Lys Trp His 
                725                 730                 735     
Gln Asn Gly Leu Val Met Asp Lys Trp Phe Ile Leu Gln Ala Thr Ser 
            740                 745                 750         
Pro Ala Ala Asn Val Leu Glu Thr Val Arg Gly Leu Leu Gln His Arg 
        755                 760                 765             
Ser Phe Thr Met Ser Asn Pro Asn Arg Ile Arg Ser Leu Ile Gly Ala 
    770                 775                 780                 
Phe Ala Gly Ser Asn Pro Ala Ala Phe His Ala Glu Asp Gly Ser Gly 
785                 790                 795                 800 
Tyr Leu Phe Leu Val Glu Met Leu Thr Asp Leu Asn Ser Arg Asn Pro 
                805                 810                 815     
Gln Val Ala Ser Arg Leu Ile Glu Pro Leu Ile Arg Leu Lys Arg Tyr 
            820                 825                 830         
Asp Ala Lys Arg Gln Glu Lys Met Arg Ala Ala Leu Glu Gln Leu Lys 
        835                 840                 845             
Gly Leu Glu Asn Leu Ser Gly Asp Leu Tyr Glu Lys Ile Thr Lys Ala 
    850                 855                 860                 
Leu Ala 
865     

<210> 17
<211> 185
<212> PRT
<213> Staphylococcus aureus


<220> 
<223> Peptide deformylase, scaffold

<400> 17
Met Leu Thr Met Lys Asp Ile Ile Arg Asp Gly His Pro Thr Leu Arg 
1               5                   10                  15      
Gln Lys Ala Ala Glu Leu Glu Leu Pro Leu Thr Lys Glu Glu Lys Glu 
            20                  25                  30          
Thr Leu Ile Ala Met Arg Glu Phe Leu Val Asn Ser Gln Asp Glu Glu 
        35                  40                  45              
Ile Ala Lys Arg Tyr Gly Leu Arg Ser Gly Val Gly Leu Ala Ala Pro 
    50                  55                  60                  
Gln Ile Asn Ile Ser Lys Arg Met Ile Ala Val Leu Ile Pro Asp Asp 
65                  70                  75                  80  
Gly Ser Gly Lys Ser Tyr Asp Tyr Met Leu Val Asn Pro Lys Ile Val 
                85                  90                  95      
Ser His Ser Val Gln Glu Ala Tyr Leu Pro Thr Gly Glu Gly Cys Leu 
            100                 105                 110         
Ser Val Asp Asp Asn Val Ala Gly Leu Val His Arg His Asn Arg Ile 
        115                 120                 125             
Thr Ile Lys Ala Lys Asp Ile Glu Gly Asn Asp Ile Gln Leu Arg Leu 
    130                 135                 140                 
Lys Gly Tyr Pro Ala Ile Val Phe Gln His Glu Ile Asp His Leu Asn 
145                 150                 155                 160 
Gly Val Met Phe Tyr Asp His Ile Asp Lys Asp His Pro Leu Gln Pro 
                165                 170                 175     
His Thr Asp Ala Val Glu Val Leu Glu 
            180                 185 

<210> 18
<211> 696
<212> PRT
<213> Homo sapiens


<220> 
<223> Glutamate carboxypeptidase II, scaffold

<400> 18
Lys His Asn Met Lys Ala Phe Leu Asp Glu Leu Lys Ala Glu Asn Ile 
1               5                   10                  15      
Lys Lys Phe Leu Tyr Asn Phe Thr Gln Ile Pro His Leu Ala Gly Thr 
            20                  25                  30          
Glu Gln Asn Phe Gln Leu Ala Lys Gln Ile Gln Ser Gln Trp Lys Glu 
        35                  40                  45              
Phe Gly Leu Asp Ser Val Glu Leu Ala His Tyr Asp Val Leu Leu Ser 
    50                  55                  60                  
Tyr Pro Asn Lys Thr His Pro Asn Tyr Ile Ser Ile Ile Asn Glu Asp 
65                  70                  75                  80  
Gly Asn Glu Ile Phe Asn Thr Ser Leu Phe Glu Pro Pro Pro Pro Gly 
                85                  90                  95      
Tyr Glu Asn Val Ser Asp Ile Val Pro Pro Phe Ser Ala Phe Ser Pro 
            100                 105                 110         
Gln Gly Met Pro Glu Gly Asp Leu Val Tyr Val Asn Tyr Ala Arg Thr 
        115                 120                 125             
Glu Asp Phe Phe Lys Leu Glu Arg Asp Met Lys Ile Asn Cys Ser Gly 
    130                 135                 140                 
Lys Ile Val Ile Ala Arg Tyr Gly Lys Val Phe Arg Gly Asn Lys Val 
145                 150                 155                 160 
Lys Asn Ala Gln Leu Ala Gly Ala Lys Gly Val Ile Leu Tyr Ser Asp 
                165                 170                 175     
Pro Ala Asp Tyr Phe Ala Pro Gly Val Lys Ser Tyr Pro Asp Gly Trp 
            180                 185                 190         
Asn Leu Pro Gly Gly Gly Val Gln Arg Gly Asn Ile Leu Asn Leu Asn 
        195                 200                 205             
Gly Ala Gly Asp Pro Leu Thr Pro Gly Tyr Pro Ala Asn Glu Tyr Ala 
    210                 215                 220                 
Tyr Arg Arg Gly Ile Ala Glu Ala Val Gly Leu Pro Ser Ile Pro Val 
225                 230                 235                 240 
His Pro Ile Gly Tyr Tyr Asp Ala Gln Lys Leu Leu Glu Lys Met Gly 
                245                 250                 255     
Gly Ser Ala Pro Pro Asp Ser Ser Trp Arg Gly Ser Leu Lys Val Pro 
            260                 265                 270         
Tyr Asn Val Gly Pro Gly Phe Thr Gly Asn Phe Ser Thr Gln Lys Val 
        275                 280                 285             
Lys Met His Ile His Ser Thr Asn Glu Val Thr Arg Ile Tyr Asn Val 
    290                 295                 300                 
Ile Gly Thr Leu Arg Gly Ala Val Glu Pro Asp Arg Tyr Val Ile Leu 
305                 310                 315                 320 
Gly Gly His Arg Asp Ser Trp Val Phe Gly Gly Ile Asp Pro Gln Ser 
                325                 330                 335     
Gly Ala Ala Val Val His Glu Ile Val Arg Ser Phe Gly Thr Leu Lys 
            340                 345                 350         
Lys Glu Gly Trp Arg Pro Arg Arg Thr Ile Leu Phe Ala Ser Trp Asp 
        355                 360                 365             
Ala Glu Glu Phe Gly Leu Leu Gly Ser Thr Glu Trp Ala Glu Glu Asn 
    370                 375                 380                 
Ser Arg Leu Leu Gln Glu Arg Gly Val Ala Tyr Ile Asn Ala Asp Ser 
385                 390                 395                 400 
Ser Ile Glu Gly Asn Tyr Thr Leu Arg Val Asp Cys Thr Pro Leu Met 
                405                 410                 415     
Tyr Ser Leu Val His Asn Leu Thr Lys Glu Leu Lys Ser Pro Asp Glu 
            420                 425                 430         
Gly Phe Glu Gly Lys Ser Leu Tyr Glu Ser Trp Thr Lys Lys Ser Pro 
        435                 440                 445             
Ser Pro Glu Phe Ser Gly Met Pro Arg Ile Ser Lys Leu Gly Ser Gly 
    450                 455                 460                 
Asn Asp Phe Glu Val Phe Phe Gln Arg Leu Gly Ile Ala Ser Gly Arg 
465                 470                 475                 480 
Ala Arg Tyr Thr Lys Asn Trp Glu Thr Asn Lys Phe Ser Gly Tyr Pro 
                485                 490                 495     
Leu Tyr His Ser Val Tyr Glu Thr Tyr Glu Leu Val Glu Lys Phe Tyr 
            500                 505                 510         
Asp Pro Met Phe Lys Tyr His Leu Thr Val Ala Gln Val Arg Gly Gly 
        515                 520                 525             
Met Val Phe Glu Leu Ala Asn Ser Ile Val Leu Pro Phe Asp Cys Arg 
    530                 535                 540                 
Asp Tyr Ala Val Val Leu Arg Lys Tyr Ala Asp Lys Ile Tyr Ser Ile 
545                 550                 555                 560 
Ser Met Lys His Pro Gln Glu Met Lys Thr Tyr Ser Val Ser Phe Asp 
                565                 570                 575     
Ser Leu Phe Ser Ala Val Lys Asn Phe Thr Glu Ile Ala Ser Lys Phe 
            580                 585                 590         
Ser Glu Arg Leu Gln Asp Phe Asp Lys Ser Asn Pro Ile Val Leu Arg 
        595                 600                 605             
Met Met Asn Asp Gln Leu Met Phe Leu Glu Arg Ala Phe Ile Asp Pro 
    610                 615                 620                 
Leu Gly Leu Pro Asp Arg Pro Phe Tyr Arg His Val Ile Tyr Ala Pro 
625                 630                 635                 640 
Ser Ser His Asn Lys Tyr Ala Gly Glu Ser Phe Pro Gly Ile Tyr Asp 
                645                 650                 655     
Ala Leu Phe Asp Ile Glu Ser Lys Val Asp Pro Ser Lys Ala Trp Gly 
            660                 665                 670         
Glu Val Lys Arg Gln Ile Tyr Val Ala Ala Phe Thr Val Gln Ala Ala 
        675                 680                 685             
Ala Glu Thr Leu Ser Glu Val Ala 
    690                 695     

<210> 19
<211> 307
<212> PRT
<213> Bos taurus


<220> 
<223> Carboxypeptidase A, scaffold

<400> 19
Ala Arg Ser Thr Asn Thr Phe Asn Tyr Ala Thr Tyr His Thr Leu Asp 
1               5                   10                  15      
Glu Ile Tyr Asp Phe Met Asp Leu Leu Val Ala Gln His Pro Glu Leu 
            20                  25                  30          
Val Ser Lys Leu Gln Ile Gly Arg Ser Tyr Glu Gly Arg Pro Ile Tyr 
        35                  40                  45              
Val Leu Lys Phe Ser Thr Gly Gly Ser Asn Arg Pro Ala Ile Trp Ile 
    50                  55                  60                  
Asp Leu Gly Ile His Ser Arg Glu Trp Ile Thr Gln Ala Thr Gly Val 
65                  70                  75                  80  
Trp Phe Ala Lys Lys Phe Thr Glu Asn Tyr Gly Gln Asn Pro Ser Phe 
                85                  90                  95      
Thr Ala Ile Leu Asp Ser Met Asp Ile Phe Leu Glu Ile Val Thr Asn 
            100                 105                 110         
Pro Asn Gly Phe Ala Phe Thr His Ser Glu Asn Arg Leu Trp Arg Lys 
        115                 120                 125             
Thr Arg Ser Val Thr Ser Ser Ser Leu Cys Val Gly Val Asp Ala Asn 
    130                 135                 140                 
Arg Asn Trp Asp Ala Gly Phe Gly Lys Ala Gly Ala Ser Ser Ser Pro 
145                 150                 155                 160 
Cys Ser Glu Thr Tyr His Gly Lys Tyr Ala Asn Ser Glu Val Glu Val 
                165                 170                 175     
Lys Ser Ile Val Asp Phe Val Lys Asn His Gly Asn Phe Lys Ala Phe 
            180                 185                 190         
Leu Ser Ile His Ser Tyr Ser Gln Leu Leu Leu Tyr Pro Tyr Gly Tyr 
        195                 200                 205             
Thr Thr Gln Ser Ile Pro Asp Lys Thr Glu Leu Asn Gln Val Ala Lys 
    210                 215                 220                 
Ser Ala Val Ala Ala Leu Lys Ser Leu Tyr Gly Thr Ser Tyr Lys Tyr 
225                 230                 235                 240 
Gly Ser Ile Ile Thr Thr Ile Tyr Gln Ala Ser Gly Gly Ser Ile Asp 
                245                 250                 255     
Trp Ser Tyr Asn Gln Gly Ile Lys Tyr Ser Phe Thr Phe Glu Leu Arg 
            260                 265                 270         
Asp Thr Gly Arg Tyr Gly Phe Leu Leu Pro Ala Ser Gln Ile Ile Pro 
        275                 280                 285             
Thr Ala Gln Glu Thr Trp Leu Gly Val Leu Thr Ile Met Glu His Thr 
    290                 295                 300                 
Val Asn Asn 
305         

<210> 20
<211> 323
<212> PRT
<213> Bos taurus


<220> 
<223> Carboxypeptidase T, scaffold

<400> 20
Asp Phe Pro Ser Tyr Asp Ser Gly Tyr His Asn Tyr Asn Glu Met Val 
1               5                   10                  15      
Asn Lys Ile Asn Thr Val Ala Ser Asn Tyr Pro Asn Ile Val Lys Lys 
            20                  25                  30          
Phe Ser Ile Gly Lys Ser Tyr Glu Gly Arg Glu Leu Trp Ala Val Lys 
        35                  40                  45              
Ile Ser Asp Asn Val Gly Thr Asp Glu Asn Glu Pro Glu Val Leu Tyr 
    50                  55                  60                  
Thr Ala Leu His His Ala Arg Glu His Leu Thr Val Glu Met Ala Leu 
65                  70                  75                  80  
Tyr Thr Leu Asp Leu Phe Thr Gln Asn Tyr Asn Leu Asp Ser Arg Ile 
                85                  90                  95      
Thr Asn Leu Val Asn Asn Arg Glu Ile Tyr Ile Val Phe Asn Ile Asn 
            100                 105                 110         
Pro Asp Gly Gly Glu Tyr Asp Ile Ser Ser Gly Ser Tyr Lys Ser Trp 
        115                 120                 125             
Arg Lys Asn Arg Gln Pro Asn Ser Gly Ser Ser Tyr Val Gly Thr Asp 
    130                 135                 140                 
Leu Asn Arg Asn Tyr Gly Tyr Lys Trp Gly Cys Cys Gly Gly Ser Ser 
145                 150                 155                 160 
Gly Ser Pro Ser Ser Glu Thr Tyr Arg Gly Arg Ser Ala Phe Ser Ala 
                165                 170                 175     
Pro Glu Thr Ala Ala Met Arg Asp Phe Ile Asn Ser Arg Val Val Gly 
            180                 185                 190         
Gly Lys Gln Gln Ile Lys Thr Leu Ile Thr Phe His Thr Tyr Ser Glu 
        195                 200                 205             
Leu Ile Leu Tyr Pro Tyr Gly Tyr Thr Tyr Thr Asp Val Pro Ser Asp 
    210                 215                 220                 
Met Thr Gln Asp Asp Phe Asn Val Phe Lys Thr Met Ala Asn Thr Met 
225                 230                 235                 240 
Ala Gln Thr Asn Gly Tyr Thr Pro Gln Gln Ala Ser Asp Leu Tyr Ile 
                245                 250                 255     
Thr Asp Gly Asp Met Thr Asp Trp Ala Tyr Gly Gln His Lys Ile Phe 
            260                 265                 270         
Ala Phe Thr Phe Glu Met Tyr Pro Thr Ser Tyr Asn Pro Gly Phe Tyr 
        275                 280                 285             
Pro Pro Asp Glu Val Ile Gly Arg Glu Thr Ser Arg Asn Lys Glu Ala 
    290                 295                 300                 
Val Leu Tyr Val Ala Glu Lys Ala Asp Cys Pro Tyr Ser Val Ile Gly 
305                 310                 315                 320 
Lys Ser Cys 
            

<210> 21
<211> 470
<212> PRT
<213> Pseudomonas aeruginosa


<220> 
<223> Alkaline protease, PAO1, scaffold

<400> 21
Gly Arg Ser Asp Ala Tyr Thr Gln Val Asp Asn Phe Leu His Ala Tyr 
1               5                   10                  15      
Ala Arg Gly Gly Asp Glu Leu Val Asn Gly His Pro Ser Tyr Thr Val 
            20                  25                  30          
Asp Gln Ala Ala Glu Gln Ile Leu Arg Glu Gln Ala Ser Trp Gln Lys 
        35                  40                  45              
Ala Pro Gly Asp Ser Val Leu Thr Leu Ser Tyr Ser Phe Leu Thr Lys 
    50                  55                  60                  
Pro Asn Asp Phe Phe Asn Thr Pro Trp Lys Tyr Val Ser Asp Ile Tyr 
65                  70                  75                  80  
Ser Leu Gly Lys Phe Ser Ala Phe Ser Ala Gln Gln Gln Ala Gln Ala 
                85                  90                  95      
Lys Leu Ser Leu Gln Ser Trp Ser Asp Val Thr Asn Ile His Phe Val 
            100                 105                 110         
Asp Ala Gly Gln Gly Asp Gln Gly Asp Leu Thr Phe Gly Asn Phe Ser 
        115                 120                 125             
Ser Ser Val Gly Gly Ala Ala Phe Ala Phe Leu Pro Asp Val Pro Asp 
    130                 135                 140                 
Ala Leu Lys Gly Gln Ser Trp Tyr Leu Ile Asn Ser Ser Tyr Ser Ala 
145                 150                 155                 160 
Asn Val Asn Pro Ala Asn Gly Asn Tyr Gly Arg Gln Thr Leu Thr His 
                165                 170                 175     
Glu Ile Gly His Thr Leu Gly Leu Ser His Pro Gly Asp Tyr Asn Ala 
            180                 185                 190         
Gly Glu Gly Asp Pro Thr Tyr Ala Asp Ala Thr Tyr Ala Glu Asp Thr 
        195                 200                 205             
Arg Ala Tyr Ser Val Met Ser Tyr Trp Glu Glu Gln Asn Thr Gly Gln 
    210                 215                 220                 
Asp Phe Lys Gly Ala Tyr Ser Ser Ala Pro Leu Leu Asp Asp Ile Ala 
225                 230                 235                 240 
Ala Ile Gln Lys Leu Tyr Gly Ala Asn Leu Thr Thr Arg Thr Gly Asp 
                245                 250                 255     
Thr Val Tyr Gly Phe Asn Ser Asn Thr Glu Arg Asp Phe Tyr Ser Ala 
            260                 265                 270         
Thr Ser Ser Ser Ser Lys Leu Val Phe Ser Val Trp Asp Ala Gly Gly 
        275                 280                 285             
Asn Asp Thr Leu Asp Phe Ser Gly Phe Ser Gln Asn Gln Lys Ile Asn 
    290                 295                 300                 
Leu Asn Glu Lys Ala Leu Ser Asp Val Gly Gly Leu Lys Gly Asn Val 
305                 310                 315                 320 
Ser Ile Ala Ala Gly Val Thr Val Glu Asn Ala Ile Gly Gly Ser Gly 
                325                 330                 335     
Ser Asp Leu Leu Ile Gly Asn Asp Val Ala Asn Val Leu Lys Gly Gly 
            340                 345                 350         
Ala Gly Asn Asp Ile Leu Tyr Gly Gly Leu Gly Ala Asp Gln Leu Trp 
        355                 360                 365             
Gly Gly Ala Gly Ala Asp Thr Phe Val Tyr Gly Asp Ile Ala Glu Ser 
    370                 375                 380                 
Ser Ala Ala Ala Pro Asp Thr Leu Arg Asp Phe Val Ser Gly Gln Asp 
385                 390                 395                 400 
Lys Ile Asp Leu Ser Gly Leu Asp Ala Phe Val Asn Gly Gly Leu Val 
                405                 410                 415     
Leu Gln Tyr Val Asp Ala Phe Ala Gly Lys Ala Gly Gln Ala Ile Leu 
            420                 425                 430         
Ser Tyr Asp Ala Ala Ser Lys Ala Gly Ser Leu Ala Ile Asp Phe Ser 
        435                 440                 445             
Gly Asp Ala His Ala Asp Phe Ala Ile Asn Leu Ile Gly Gln Ala Thr 
    450                 455                 460                 
Gln Ala Asp Ile Val Val 
465                 470 

<210> 22
<211> 323
<212> PRT
<213> Thermoactinomyces vulgaris


<220> 
<223> Carboxypeptidase T, scaffold

<400> 22
Asp Phe Pro Ser Tyr Asp Ser Gly Tyr His Asn Tyr Asn Glu Met Val 
1               5                   10                  15      
Asn Lys Ile Asn Thr Val Ala Ser Asn Tyr Pro Asn Ile Val Lys Lys 
            20                  25                  30          
Phe Ser Ile Gly Lys Ser Tyr Glu Gly Arg Glu Leu Trp Ala Val Lys 
        35                  40                  45              
Ile Ser Asp Asn Val Gly Thr Asp Glu Asn Glu Pro Glu Val Leu Tyr 
    50                  55                  60                  
Thr Ala Leu His His Ala Arg Glu His Leu Thr Val Glu Met Ala Leu 
65                  70                  75                  80  
Tyr Thr Leu Asp Leu Phe Thr Gln Asn Tyr Asn Leu Asp Ser Arg Ile 
                85                  90                  95      
Thr Asn Leu Val Asn Asn Arg Glu Ile Tyr Ile Val Phe Asn Ile Asn 
            100                 105                 110         
Pro Asp Gly Gly Glu Tyr Asp Ile Ser Ser Gly Ser Tyr Lys Ser Trp 
        115                 120                 125             
Arg Lys Asn Arg Gln Pro Asn Ser Gly Ser Ser Tyr Val Gly Thr Asp 
    130                 135                 140                 
Leu Asn Arg Asn Tyr Gly Tyr Lys Trp Gly Cys Cys Gly Gly Ser Ser 
145                 150                 155                 160 
Gly Ser Pro Ser Ser Glu Thr Tyr Arg Gly Arg Ser Ala Phe Ser Ala 
                165                 170                 175     
Pro Glu Thr Ala Ala Met Arg Asp Phe Ile Asn Ser Arg Val Val Gly 
            180                 185                 190         
Gly Lys Gln Gln Ile Lys Thr Leu Ile Thr Phe His Thr Tyr Ser Glu 
        195                 200                 205             
Leu Ile Leu Tyr Pro Tyr Gly Tyr Thr Tyr Thr Asp Val Pro Ser Asp 
    210                 215                 220                 
Met Thr Gln Asp Asp Phe Asn Val Phe Lys Thr Met Ala Asn Thr Met 
225                 230                 235                 240 
Ala Gln Thr Asn Gly Tyr Thr Pro Gln Gln Ala Ser Asp Leu Tyr Ile 
                245                 250                 255     
Thr Asp Gly Asp Met Thr Asp Trp Ala Tyr Gly Gln His Lys Ile Phe 
            260                 265                 270         
Ala Phe Thr Phe Glu Met Tyr Pro Thr Ser Tyr Asn Pro Gly Phe Tyr 
        275                 280                 285             
Pro Pro Asp Glu Val Ile Gly Arg Glu Thr Ser Arg Asn Lys Glu Ala 
    290                 295                 300                 
Val Leu Tyr Val Ala Glu Lys Ala Asp Cys Pro Tyr Ser Val Ile Gly 
305                 310                 315                 320 
Lys Ser Cys 
            

<210> 23
<211> 860
<212> PRT
<213> Escherichia coli


<220> 
<223> StcE, scaffold

<400> 23
Asn Ser Ala Ile Tyr Phe Asn Thr Ser Gln Pro Ile Asn Asp Leu Gln 
1               5                   10                  15      
Gly Ser Leu Ala Ala Glu Val Lys Phe Ala Gln Ser Gln Ile Leu Pro 
            20                  25                  30          
Ala His Pro Lys Glu Gly Asp Ser Gln Pro His Leu Thr Ser Leu Arg 
        35                  40                  45              
Lys Ser Leu Leu Leu Val Arg Pro Val Lys Ala Asp Asp Lys Thr Pro 
    50                  55                  60                  
Val Gln Val Glu Ala Arg Asp Asp Asn Asn Lys Ile Leu Gly Thr Leu 
65                  70                  75                  80  
Thr Leu Tyr Pro Pro Ser Ser Leu Pro Asp Thr Ile Tyr His Leu Asp 
                85                  90                  95      
Gly Val Pro Glu Gly Gly Ile Asp Phe Thr Pro His Asn Gly Thr Lys 
            100                 105                 110         
Lys Ile Ile Asn Thr Val Ala Glu Val Asn Lys Leu Ser Asp Ala Ser 
        115                 120                 125             
Gly Ser Ser Ile His Ser His Leu Thr Asn Asn Ala Leu Val Glu Ile 
    130                 135                 140                 
His Thr Ala Asn Gly Arg Trp Val Arg Asp Ile Tyr Leu Pro Gln Gly 
145                 150                 155                 160 
Pro Asp Leu Glu Gly Lys Met Val Arg Phe Val Ser Ser Ala Gly Tyr 
                165                 170                 175     
Ser Ser Thr Val Phe Tyr Gly Asp Arg Lys Val Thr Leu Ser Val Gly 
            180                 185                 190         
Asn Thr Leu Leu Phe Lys Tyr Val Asn Gly Gln Trp Phe Arg Ser Gly 
        195                 200                 205             
Glu Leu Glu Asn Asn Arg Ile Thr Tyr Ala Gln His Ile Trp Ser Ala 
    210                 215                 220                 
Glu Leu Pro Ala His Trp Ile Val Pro Gly Leu Asn Leu Val Ile Lys 
225                 230                 235                 240 
Gln Gly Asn Leu Ser Gly Arg Leu Asn Asp Ile Lys Ile Gly Ala Pro 
                245                 250                 255     
Gly Glu Leu Leu Leu His Thr Ile Asp Ile Gly Met Leu Thr Thr Pro 
            260                 265                 270         
Arg Asp Arg Phe Asp Phe Ala Ala Asp Ala Ala Ala His Arg Glu Tyr 
        275                 280                 285             
Phe Gln Thr Ile Pro Val Ser Arg Met Ile Val Asn Asn Tyr Ala Pro 
    290                 295                 300                 
Leu His Leu Lys Glu Val Met Leu Pro Thr Gly Glu Leu Leu Thr Asp 
305                 310                 315                 320 
Met Asp Pro Gly Asn Gly Gly Trp His Ser Gly Thr Met Arg Gln Arg 
                325                 330                 335     
Ile Gly Lys Glu Leu Val Ser His Gly Ile Asp Asn Ala Asn Tyr Gly 
            340                 345                 350         
Leu Asn Ser Thr Ala Gly Leu Gly Glu Asn Ser His Pro Tyr Val Val 
        355                 360                 365             
Ala Gln Leu Ala Ala His Asn Ser Arg Gly Asn Tyr Ala Asn Gly Ile 
    370                 375                 380                 
Gln Val His Gly Gly Ser Gly Gly Gly Gly Ile Val Thr Leu Asp Ser 
385                 390                 395                 400 
Thr Leu Gly Asn Glu Phe Ser His Asp Val Gly His Asn Tyr Gly Leu 
                405                 410                 415     
Gly His Tyr Val Asp Gly Phe Lys Gly Ser Val His Arg Ser Ala Glu 
            420                 425                 430         
Asn Asn Asn Ser Thr Trp Gly Trp Asp Gly Asp Lys Lys Arg Phe Ile 
        435                 440                 445             
Pro Asn Phe Tyr Pro Ser Gln Thr Asn Glu Lys Ser Cys Leu Asn Asn 
    450                 455                 460                 
Gln Cys Gln Glu Pro Phe Asp Gly His Lys Phe Gly Phe Asp Ala Met 
465                 470                 475                 480 
Ala Gly Gly Ser Pro Phe Ser Ala Ala Asn Arg Phe Thr Met Tyr Thr 
                485                 490                 495     
Pro Asn Ser Ser Ala Ile Ile Gln Arg Phe Phe Glu Asn Lys Ala Val 
            500                 505                 510         
Phe Asp Ser Arg Ser Ser Thr Gly Phe Ser Lys Trp Asn Ala Asp Thr 
        515                 520                 525             
Gln Glu Met Glu Pro Tyr Glu His Thr Ile Asp Arg Ala Glu Gln Ile 
    530                 535                 540                 
Thr Ala Ser Val Asn Glu Leu Ser Glu Ser Lys Met Ala Glu Leu Met 
545                 550                 555                 560 
Ala Glu Tyr Ala Val Val Lys Val His Met Trp Asn Gly Asn Trp Thr 
                565                 570                 575     
Arg Asn Ile Tyr Ile Pro Thr Ala Ser Ala Asp Asn Arg Gly Ser Ile 
            580                 585                 590         
Leu Thr Ile Asn His Glu Ala Gly Tyr Asn Ser Tyr Leu Phe Ile Asn 
        595                 600                 605             
Gly Asp Glu Lys Val Val Ser Gln Gly Tyr Lys Lys Ser Phe Val Ser 
    610                 615                 620                 
Asp Gly Gln Phe Trp Lys Glu Arg Asp Val Val Asp Thr Arg Glu Ala 
625                 630                 635                 640 
Arg Lys Pro Glu Gln Phe Gly Val Pro Val Thr Thr Leu Val Gly Tyr 
                645                 650                 655     
Tyr Asp Pro Glu Gly Thr Leu Ser Ser Tyr Ile Tyr Pro Ala Met Tyr 
            660                 665                 670         
Gly Ala Tyr Gly Phe Thr Tyr Ser Asp Asp Ser Gln Asn Leu Ser Asp 
        675                 680                 685             
Asn Asp Cys Gln Leu Gln Val Asp Thr Lys Glu Gly Gln Leu Arg Phe 
    690                 695                 700                 
Arg Leu Ala Asn His Arg Ala Asn Asn Thr Val Met Asn Lys Phe His 
705                 710                 715                 720 
Ile Asn Val Pro Thr Glu Ser Gln Pro Thr Gln Ala Thr Leu Val Cys 
                725                 730                 735     
Asn Asn Lys Ile Leu Asp Thr Lys Ser Leu Thr Pro Ala Pro Glu Gly 
            740                 745                 750         
Leu Thr Tyr Thr Val Asn Gly Gln Ala Leu Pro Ala Lys Glu Asn Glu 
        755                 760                 765             
Gly Cys Ile Val Ser Val Asn Ser Gly Lys Arg Tyr Cys Leu Pro Val 
    770                 775                 780                 
Gly Gln Arg Ser Gly Tyr Ser Leu Pro Asp Trp Ile Val Gly Gln Glu 
785                 790                 795                 800 
Val Tyr Val Asp Ser Gly Ala Lys Ala Lys Val Leu Leu Ser Asp Trp 
                805                 810                 815     
Asp Asn Leu Ser Tyr Asn Arg Ile Gly Glu Phe Val Gly Asn Val Asn 
            820                 825                 830         
Pro Ala Asp Met Lys Lys Val Lys Ala Trp Asn Gly Gln Tyr Leu Asp 
        835                 840                 845             
Phe Ser Lys Pro Arg Ser Met Arg Val Val Tyr Lys 
    850                 855                 860 

<210> 24
<211> 259
<212> PRT
<213> Escherichia coli


<220> 
<223> AB5 holotoxin, scaffold

<400> 24
Met Ala Glu Arg Thr Pro Asn Glu Glu Lys Lys Val Ile Gly Tyr Ala 
1               5                   10                  15      
Asp His Asn Gly Gln Leu Tyr Asn Ile Thr Ser Ile Tyr Gly Pro Val 
            20                  25                  30          
Ile Asn Tyr Thr Val Pro Asp Glu Asn Ile Thr Ile Asn Thr Ile Asn 
        35                  40                  45              
Ser Thr Gly Glu Arg Thr Gln Leu Thr Ile Asn Tyr Ser Asp Tyr Val 
    50                  55                  60                  
Arg Glu Ala Phe Asn Glu Trp Ala Pro Ser Gly Ile Arg Val Gln Gln 
65                  70                  75                  80  
Val Ser Ser Ser Gly Ala Glu Ala Arg Val Val Ser Phe Ser Thr Thr 
                85                  90                  95      
Asn Tyr Ala Asp Asn Ser Leu Gly Ser Thr Ile Phe Asp Pro Ser Gly 
            100                 105                 110         
Asn Ser Arg Thr Arg Ile Asp Ile Gly Ser Phe Asn Arg Ile Val Met 
        115                 120                 125             
Asn Asn Phe Glu Lys Leu Lys Ser Arg Gly Ala Ile Pro Ala Asn Met 
    130                 135                 140                 
Ser Pro Glu Glu Tyr Ile Lys Leu Lys Leu Arg Ile Thr Ile Lys His 
145                 150                 155                 160 
Glu Ile Gly His Ile Leu Gly Leu Leu His Asn Asn Glu Gly Gly Ser 
                165                 170                 175     
Tyr Phe Pro His Gly Val Gly Leu Glu Val Ala Arg Cys Arg Leu Leu 
            180                 185                 190         
Asn Gln Ala Pro Ser Ile Met Leu Asn Gly Ser Asn Tyr Asp Tyr Ile 
        195                 200                 205             
Asp Arg Leu Ser His Tyr Leu Glu Arg Pro Val Thr Glu Thr Asp Ile 
    210                 215                 220                 
Gly Pro Ser Arg Asn Asp Ile Glu Gly Val Arg Val Met Arg Arg Gly 
225                 230                 235                 240 
Gly Ser Gly Asn Ser Phe Thr Asn Arg Phe Ser Cys Leu Gly Leu Gly 
                245                 250                 255     
Leu Ala Phe 
            

<210> 25
<211> 249
<212> PRT
<213> Saccharopolyspora


<220> 
<223> ZE2 (TIM barrel), scaffold

<400> 25
Gly Ser Pro Arg Tyr Leu Lys Gly Trp Leu Lys Asp Val Val Gln Leu 
1               5                   10                  15      
Ser Leu Arg Arg Pro Ser Phe Arg Ala Ser Arg Gln Arg Pro Ile Ile 
            20                  25                  30          
Ser Leu Asn Glu Arg Ile Leu Glu Phe Asn Lys Arg Asn Ile Thr Ala 
        35                  40                  45              
Ile Ile Ala Glu Tyr Lys Arg Lys Ser Pro Ser Gly Leu Asp Val Glu 
    50                  55                  60                  
Arg Asp Pro Ile Glu Tyr Ser Lys Phe Met Glu Arg Tyr Ala Val Gly 
65                  70                  75                  80  
Leu Ser Ile Leu Thr Glu Glu Lys Tyr Phe Asn Gly Ser Tyr Glu Thr 
                85                  90                  95      
Leu Arg Lys Ile Ala Ser Ser Val Ser Ile Pro Ile Leu Met Ala Asp 
            100                 105                 110         
Phe Ile Val Lys Glu Ser Gln Ile Asp Asp Ala Tyr Asn Leu Gly Ala 
        115                 120                 125             
Asp Thr Val Leu Leu Ile Val Lys Ile Leu Thr Glu Arg Glu Leu Glu 
    130                 135                 140                 
Ser Leu Leu Glu Tyr Ala Arg Ser Tyr Gly Met Glu Pro Leu Ile Gly 
145                 150                 155                 160 
Ile Asn Asp Glu Asn Asp Leu Asp Ile Ala Leu Arg Ile Gly Ala Arg 
                165                 170                 175     
Phe Ile Gly Ile His Ser Ala Asp His Glu Thr Leu Glu Ile Asn Lys 
            180                 185                 190         
Glu Asn Gln Arg Lys Leu Ile Ser Met Ile Pro Ser Asn Val Val Lys 
        195                 200                 205             
Val Ala Ala His Gly Ile Ser Glu Arg Asn Glu Ile Glu Glu Leu Arg 
    210                 215                 220                 
Lys Leu Gly Val Asn Ala Phe Leu Ile Gly Ser Ser Leu Met Arg Asn 
225                 230                 235                 240 
Pro Glu Lys Ile Lys Glu Phe Ile Leu 
                245                 

<210> 26
<211> 94
<212> PRT
<213> Artificial Sequence


<220> 
<223> MID1sc10 (de novo helical bundle), scaffold

<400> 26
Gly Ser Pro Leu Ala Gln Gln Ile Lys Asn Thr Leu Thr Phe Ile Gly 
1               5                   10                  15      
Gln Ala Asn Ala Ala Gly Arg Met Asp Glu Val Arg Thr Leu Gln Gln 
            20                  25                  30          
Asn Leu His Pro Leu Trp Ala Glu Tyr Phe Gln Gln Thr Glu Gly Ser 
        35                  40                  45              
Gly Gly Ser Pro Leu Ala Gln Gln Ile Gln Tyr Gly His Val Leu Ile 
    50                  55                  60                  
His Gln Ala Arg Ala Ala Gly Arg Met Asp Glu Val Arg Arg Leu Ser 
65                  70                  75                  80  
Glu Asn Thr Leu Gln Leu Met Lys Glu Tyr Phe Gln Gln Ser 
                85                  90                  

<210> 27
<211> 287
<212> PRT
<213> Burkholderia multivorans ATCC 17616


<220> 
<223> Amidohydrolase, scaffold

<400> 27
Ala Leu Arg Ile Asp Ser His Gln His Phe Trp Arg Tyr Arg Ala Ala 
1               5                   10                  15      
Asp Tyr Pro Trp Ile Gly Ala Gly Met Gly Val Leu Ala Arg Asp Tyr 
            20                  25                  30          
Leu Pro Asp Ala Leu His Pro Leu Met His Ala Gln Ala Leu Gly Ala 
        35                  40                  45              
Ser Ile Ala Val Gln Ala Arg Ala Gly Arg Asp Glu Thr Ala Phe Leu 
    50                  55                  60                  
Leu Glu Leu Ala Cys Asp Glu Ala Arg Ile Ala Ala Val Val Gly Trp 
65                  70                  75                  80  
Glu Asp Leu Arg Ala Pro Gln Leu Ala Glu Arg Val Ala Glu Trp Arg 
                85                  90                  95      
Gly Thr Lys Leu Arg Gly Phe Arg His Gln Leu Gln Asp Glu Ala Asp 
            100                 105                 110         
Val Arg Ala Phe Val Asp Asp Ala Asp Phe Ala Arg Gly Val Ala Trp 
        115                 120                 125             
Leu Gln Ala Asn Asp Tyr Val Tyr Asp Val Leu Val Phe Glu Arg Gln 
    130                 135                 140                 
Leu Pro Asp Val Gln Ala Phe Cys Ala Arg His Asp Ala His Trp Leu 
145                 150                 155                 160 
Val Leu Asp His Ala Gly Lys Pro Ala Leu Ala Glu Phe Asp Arg Asp 
                165                 170                 175     
Asp Thr Ala Leu Ala Arg Trp Arg Ala Ala Leu Arg Glu Leu Ala Ala 
            180                 185                 190         
Leu Pro His Val Val Cys Lys Leu Ser Gly Leu Val Thr Glu Ala Asp 
        195                 200                 205             
Trp Arg Arg Gly Leu Arg Ala Ser Asp Leu Arg His Ile Glu Gln Cys 
    210                 215                 220                 
Leu Asp Ala Ala Leu Asp Ala Phe Gly Pro Gln Arg Leu Met Phe Gly 
225                 230                 235                 240 
Ser Asp Trp Pro Val Cys Leu Leu Ala Ala Ser Tyr Asp Glu Val Ala 
                245                 250                 255     
Ser Leu Val Glu Arg Trp Ala Glu Ser Arg Leu Ser Ala Ala Glu Arg 
            260                 265                 270         
Ser Ala Leu Trp Gly Gly Thr Ala Ala Arg Cys Tyr Ala Leu Pro 
        275                 280                 285         

<210> 28
<211> 185
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 3U7M; mutant; M64

<400> 28
Met Leu Thr Met Lys Asp Ile Ile Arg Asp Gly His Pro Thr Leu Arg 
1               5                   10                  15      
Gln Lys Ala Ala Glu Leu Glu Leu Pro Leu Thr Lys Glu Glu Lys Glu 
            20                  25                  30          
Thr Leu Ile Ala Met Arg Glu Phe Leu Val Asn Ser Gln Asp Glu Glu 
        35                  40                  45              
Ile Ala Lys Arg Tyr Gly Leu Arg Ser Ala Val Val Ile Val Ala Pro 
    50                  55                  60                  
Met Ile Asn Ile Ser Lys Arg Met Ile Ala Val Leu Leu Pro Asp Asp 
65                  70                  75                  80  
Gly Ser Gly Lys Ser Tyr Asp Tyr Met Leu Val Asn Pro Lys Ile Val 
                85                  90                  95      
Ser His Ser Val Gln Glu Ala Tyr Leu Pro Asp Gly Leu Gln Cys Leu 
            100                 105                 110         
Ser Val Asp Asp Asn Val Ala Gly Leu Val His Arg His Asn Arg Ile 
        115                 120                 125             
Thr Ile Lys Ala Lys Asp Ile Glu Gly Asn Asp Ile Gln Leu Arg Leu 
    130                 135                 140                 
Lys Gly Leu Pro Ala Ile Leu Phe Gln His Ala Ile Asp His Leu Asn 
145                 150                 155                 160 
Gly Val Met Phe Tyr Asp His Ile Asp Lys Asp His Pro Leu Gln Pro 
                165                 170                 175     
His Thr Asp Ala Val Glu Val Leu Leu 
            180                 185 

<210> 29
<211> 470
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 1KAP; mutant; M64

<400> 29
Gly Arg Ser Asp Ala Tyr Thr Gln Val Asp Asn Phe Leu His Ala Tyr 
1               5                   10                  15      
Ala Arg Gly Gly Asp Glu Leu Val Asn Gly His Pro Ser Tyr Thr Val 
            20                  25                  30          
Asp Gln Ala Ala Glu Gln Ile Leu Arg Glu Gln Ala Ser Trp Gln Lys 
        35                  40                  45              
Ala Pro Gly Asp Ser Val Leu Thr Leu Ser Tyr Ser Phe Leu Thr Lys 
    50                  55                  60                  
Pro Asn Asp Phe Phe Asn Thr Pro Trp Lys Tyr Val Ser Asp Ile Tyr 
65                  70                  75                  80  
Ser Leu Gly Lys Phe Ser Ala Phe Ser Ala Gln Gln Gln Ala Gln Ala 
                85                  90                  95      
Lys Leu Ser Leu Gln Ser Trp Ser Asp Val Thr Asn Ile His Phe Val 
            100                 105                 110         
Asp Ala Gly Gln Gly Asp Gln Gly Asp Leu Thr Phe Gly Asn Phe Ser 
        115                 120                 125             
Ser Ser Val Gly Gly Leu Val Phe Val Phe Leu Pro Asp Val Pro Asp 
    130                 135                 140                 
Ala Leu Lys Gly Gln Ser Trp Tyr Leu Ile Asn Ser Ser Trp Ser Ile 
145                 150                 155                 160 
Val Val Asn Pro Ala Asn Gly Asn Arg Gly Arg Gln Leu Leu Thr His 
                165                 170                 175     
Met Ile Gly His Thr Leu Gly Leu Ser His Pro Gly Asp Tyr His Pro 
            180                 185                 190         
Gly Glu Gly Asp Pro Thr Tyr Ala Asp Ala Thr Tyr Ala Glu Asp Thr 
        195                 200                 205             
Leu Ala Tyr Ser Val Met Ser Leu Trp Glu Glu Gln Asn Thr Gly Gln 
    210                 215                 220                 
Asp Phe Lys Gly Ala Tyr Ser Ser Ala Pro Leu Leu Asp Asp Ile Ala 
225                 230                 235                 240 
Ala Ile Gln Lys Leu Tyr Gly Ala Asn Leu Thr Thr Arg Thr Gly Asp 
                245                 250                 255     
Thr Val Tyr Gly Phe Asn Ser Asn Thr Glu Arg Asp Phe Tyr Ser Ala 
            260                 265                 270         
Thr Ser Ser Ser Ser Lys Leu Val Phe Ser Val Trp Asp Ala Gly Gly 
        275                 280                 285             
Asn Asp Thr Leu Asp Phe Ser Gly Phe Ser Gln Asn Gln Lys Ile Asn 
    290                 295                 300                 
Leu Asn Glu Lys Ala Leu Ser Asp Val Gly Gly Leu Lys Gly Asn Val 
305                 310                 315                 320 
Ser Ile Ala Ala Gly Val Thr Val Glu Asn Ala Ile Gly Gly Ser Gly 
                325                 330                 335     
Ser Asp Leu Leu Ile Gly Asn Asp Val Ala Asn Val Leu Lys Gly Gly 
            340                 345                 350         
Ala Gly Asn Asp Ile Leu Tyr Gly Gly Leu Gly Ala Asp Gln Leu Trp 
        355                 360                 365             
Gly Gly Ala Gly Ala Asp Thr Phe Val Tyr Gly Asp Ile Ala Glu Ser 
    370                 375                 380                 
Ser Ala Ala Ala Pro Asp Thr Leu Arg Asp Phe Val Ser Gly Gln Asp 
385                 390                 395                 400 
Lys Ile Asp Leu Ser Gly Leu Asp Ala Phe Val Asn Gly Gly Leu Val 
                405                 410                 415     
Leu Gln Tyr Val Asp Ala Phe Ala Gly Lys Ala Gly Gln Ala Ile Leu 
            420                 425                 430         
Ser Tyr Asp Ala Ala Ser Lys Ala Gly Ser Leu Ala Ile Asp Phe Ser 
        435                 440                 445             
Gly Asp Ala His Ala Asp Phe Ala Ile Asn Leu Ile Gly Gln Ala Thr 
    450                 455                 460                 
Gln Ala Asp Ile Val Val 
465                 470 

<210> 30
<211> 182
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 2X7M; mutant; M64

<400> 30
Leu Val Pro Arg Gly Ser His Met Lys Leu Cys Leu Val Ala Phe Asp 
1               5                   10                  15      
Gly Arg Ile Pro Met Leu Ser Ser Ile Val Asp Arg Phe Glu Glu His 
            20                  25                  30          
Val Ser Glu Tyr Leu Gly Glu Val Lys Val Lys Lys Lys Arg Ala Lys 
        35                  40                  45              
Leu Pro Glu His Ala Tyr Ser Lys Val Arg Gly Gln Tyr Leu Ala Arg 
    50                  55                  60                  
Ala Leu Leu Asp Thr Leu Arg Gly Met Lys Gly Glu Tyr Asp Arg Val 
65                  70                  75                  80  
Leu Gly Leu Thr Ser Glu Asp Leu Tyr Ile Pro Gly Val Asn Phe Val 
                85                  90                  95      
Phe Leu Ile Ala Arg Cys Pro Gly Arg Glu Ala Val Val Ser Val Ala 
            100                 105                 110         
Arg Leu Leu Asp Pro Asp Pro Glu Leu Tyr Leu Glu Arg Val Val Lys 
        115                 120                 125             
Glu Leu Thr His Ala Leu Gly His Thr Phe Gly Leu Gly His Cys Pro 
    130                 135                 140                 
Asp Arg Asn Cys Val Met Ser Pro Ala Ser Ser Leu Leu Glu Val Asp 
145                 150                 155                 160 
Arg Lys Ser Pro Asn Phe Cys Arg Arg Cys Thr Glu Leu Leu Gln Arg 
                165                 170                 175     
Asn Leu Lys Arg Gly Gly 
            180         

<210> 31
<211> 478
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 1LML; mutant; M64

<400> 31
Val Val Arg Asp Val Asn Trp Gly Ala Leu Arg Ile Ala Val Ser Thr 
1               5                   10                  15      
Glu Asp Leu Thr Asp Pro Ala Tyr His Cys Ala Arg Val Gly Gln His 
            20                  25                  30          
Val Lys Asp His Ala Gly Ala Ile Val Thr Cys Thr Ala Glu Asp Ile 
        35                  40                  45              
Leu Thr Asn Glu Lys Arg Asp Ile Leu Val Lys His Leu Ile Pro Gln 
    50                  55                  60                  
Ala Val Gln Leu His Thr Glu Arg Leu Lys Val Gln Gln Val Gln Gly 
65                  70                  75                  80  
Lys Trp Lys Val Thr Asp Met Val Gly Asp Ile Cys Gly Asp Phe Lys 
                85                  90                  95      
Val Pro Gln Ala His Ile Thr Glu Gly Phe Ser Asn Thr Asp Phe Val 
            100                 105                 110         
Met Tyr Val Ala Ser Val Pro Ser Glu Glu Gly Val Leu Ala Trp Ala 
        115                 120                 125             
Thr Thr Cys Gln Thr Phe Ser Asp Gly His Pro Ala Val Gly Val Ile 
    130                 135                 140                 
Asn Ile Pro Ala Ala Asn Ile Ala Ser Arg Tyr Asp Gln Leu Val Thr 
145                 150                 155                 160 
Arg Val Val Thr His Val Met Ala His Ala Leu Gly Phe Ser Gly Pro 
                165                 170                 175     
Phe Phe Glu Asp Ala Arg Ile Val Ala Asn Val Pro Asn Val Arg Gly 
            180                 185                 190         
Lys Asn Phe Asp Val Pro Val Ile Asn Ser Ser Thr Ala Val Ala Lys 
        195                 200                 205             
Ala Arg Glu Gln Tyr Gly Cys Asp Thr Leu Glu Tyr Leu Glu Val Glu 
    210                 215                 220                 
Asp Gln Gly Gly Glu Gly Tyr Ala Gly Ser His Ile Lys Met Arg Asn 
225                 230                 235                 240 
Ala Gln Asp Glu Leu Met Ala Pro Ala Ala Ala Ala Gly Tyr Tyr Thr 
                245                 250                 255     
Ala Leu Thr Met Ala Ile Phe Gln Asp Leu Gly Phe Tyr Gln Ala Asp 
            260                 265                 270         
Phe Ser Lys Ala Glu Val Met Pro Trp Gly Gln Asn Ala Gly Cys Ala 
        275                 280                 285             
Phe Leu Thr Asn Lys Cys Met Glu Gln Ser Val Thr Gln Trp Pro Ala 
    290                 295                 300                 
Met Phe Cys Asn Glu Ser Glu Asp Ala Ile Arg Cys Pro Thr Ser Arg 
305                 310                 315                 320 
Leu Ser Leu Gly Ala Cys Gly Val Thr Arg His Pro Gly Leu Pro Pro 
                325                 330                 335     
Tyr Trp Gln Tyr Phe Thr Asp Pro Ser Leu Ala Gly Val Ser Ala Leu 
            340                 345                 350         
Met Asp Tyr Cys Pro Val Val Val Pro Tyr Ser Asp Gly Ser Cys Thr 
        355                 360                 365             
Gln Arg Ala Ser Glu Ala His Ala Ser Leu Leu Pro Phe Asn Val Phe 
    370                 375                 380                 
Ser Asp Ala Ala Arg Cys Ile Asp Gly Ala Phe Arg Pro Lys Ala Thr 
385                 390                 395                 400 
Asp Gly Ile Val Lys Ser Tyr Ala Gly Leu Cys Ala Asn Val Gln Cys 
                405                 410                 415     
Asp Thr Ala Thr Arg Thr Tyr Ser Val Gln Val His Gly Ser Asn Asp 
            420                 425                 430         
Tyr Thr Asn Cys Thr Pro Gly Leu Arg Val Glu Leu Ser Thr Val Ser 
        435                 440                 445             
Asn Ala Phe Glu Gly Gly Gly Tyr Ile Thr Cys Pro Pro Tyr Val Glu 
    450                 455                 460                 
Val Cys Gln Gly Asn Val Gln Ala Ala Lys Asp Gly Gly Asn 
465                 470                 475             

<210> 32
<211> 866
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 4Q4E; mutant; M65

<400> 32
Pro Gln Ala Lys Tyr Arg His Asp Tyr Arg Ala Pro Asp Tyr Gln Ile 
1               5                   10                  15      
Thr Asp Ile Asp Leu Thr Phe Asp Leu Asp Ala Gln Lys Thr Val Val 
            20                  25                  30          
Thr Ala Val Ser Gln Ala Val Arg His Gly Ala Ser Asp Ala Pro Leu 
        35                  40                  45              
Arg Leu Asn Gly Glu Asp Leu Lys Leu Val Ser Val His Ile Asn Asp 
    50                  55                  60                  
Glu Pro Trp Thr Ala Trp Lys Glu Glu Glu Gly Ala Leu Val Ile Ser 
65                  70                  75                  80  
Asn Leu Pro Glu Arg Phe Thr Leu Lys Ile Ile Asn Glu Ile Ser Pro 
                85                  90                  95      
Ala Ala Asn Thr Ala Leu Glu Gly Leu Tyr Gln Ser Gly Asp Ala Leu 
            100                 105                 110         
Cys Thr Gln Cys Leu Ala Glu Gly Phe Arg His Ile Thr Tyr Tyr Leu 
        115                 120                 125             
Asp Arg Pro Asp Val Leu Ala Arg Phe Thr Thr Lys Ile Ile Ala Asp 
    130                 135                 140                 
Lys Ile Lys Tyr Pro Phe Leu Leu Ser Asn Gly Asn Arg Val Ala Gln 
145                 150                 155                 160 
Gly Glu Leu Glu Asn Gly Arg His Trp Val Gln Trp Gln Asp Pro Phe 
                165                 170                 175     
Pro Lys Pro Cys Tyr Leu Phe Ala Leu Val Ala Gly Asp Phe Asp Val 
            180                 185                 190         
Leu Arg Asp Thr Phe Thr Thr Arg Ser Gly Arg Glu Val Ala Leu Glu 
        195                 200                 205             
Leu Tyr Val Asp Arg Gly Asn Leu Asp Arg Ala Pro Trp Ala Met Thr 
    210                 215                 220                 
Ser Leu Lys Asn Ser Met Lys Trp Asp Glu Glu Arg Phe Gly Leu Glu 
225                 230                 235                 240 
Tyr Asp Leu Asp Ile Tyr Met Ile Val Ala Val Asp Phe Leu Asn Thr 
                245                 250                 255     
Gly Val Val Pro Asn Lys Gly Leu Asn Ile Phe Asn Ser Ala Leu Val 
            260                 265                 270         
Leu Ala Arg Thr Asp Thr Ala Thr Asp Lys Asp Tyr Leu Glu Ile Glu 
        275                 280                 285             
Ala Val Ile Gly His Val Tyr Phe His Asn Trp Thr Gly Asn Arg Val 
    290                 295                 300                 
Thr Cys Arg Asp Trp Phe Gln Leu Ser Leu Ile Glu Gly Leu Thr Leu 
305                 310                 315                 320 
Phe Arg Ile Gln Glu Phe Ser Ser Asp Leu Gly Ser Arg Ala Val Asn 
                325                 330                 335     
Arg Ile Asn Asn Val Arg Thr Met Arg Gly Leu Gln Phe Ala Glu Asp 
            340                 345                 350         
Ala Ser Pro Met Ala His Pro Ile Arg Pro Asp Met Val Ile Glu Met 
        355                 360                 365             
Asn Asn Phe Phe Thr Leu Thr Val Leu Trp Lys Gly Ala Glu Val Ile 
    370                 375                 380                 
Arg Met Ile His Thr Leu Leu Gly Glu Glu Asn Phe Gln Lys Gly Met 
385                 390                 395                 400 
Gln Leu Tyr Phe Glu Arg His Asp Gly Ser Ala Ala Thr Cys Asp Asp 
                405                 410                 415     
Phe Val Gln Ala Met Glu Asp Ala Ser Asn Val Asp Leu Ser His Phe 
            420                 425                 430         
Arg Arg Trp Tyr Ser Gln Ser Gly Thr Pro Ile Val Thr Val Lys Asp 
        435                 440                 445             
Asp Tyr Asn Pro Glu Thr Glu Gln Tyr Thr Leu Thr Ile Ser Gln Arg 
    450                 455                 460                 
Thr Pro Ala Thr Pro Asp Gln Ala Glu Lys Gln Pro Leu His Ile Pro 
465                 470                 475                 480 
Phe Ala Ile Glu Leu Tyr Asp Asn Glu Gly Lys Val Ile Pro Leu Gln 
                485                 490                 495     
Lys Gly Gly His Pro Val Asn Ser Val Leu Asn Val Thr Gln Ala Glu 
            500                 505                 510         
Gln Thr Phe Val Phe Asp Asn Val Tyr Phe Gln Pro Val Pro Ala Leu 
        515                 520                 525             
Leu Cys Glu Phe Ser Ala Pro Val Lys Leu Glu Tyr Lys Trp Ser Asp 
    530                 535                 540                 
Gln Gln Leu Thr Phe Leu Met Arg His Ala Arg Asn Asp Phe Ser Arg 
545                 550                 555                 560 
Trp Asp Ala Ala Gln Ser Leu Leu Ala Thr Tyr Ile Lys Leu Asn Val 
                565                 570                 575     
Ala Arg His Gln Gln Gly Gln Pro Leu Ser Leu Pro Val His Val Ala 
            580                 585                 590         
Asp Ala Phe Arg Ala Val Leu Leu Asp Glu Lys Ile Asp Pro Ala Leu 
        595                 600                 605             
Ala Ala Glu Ile Leu Thr Leu Pro Ser Val Asn Glu Met Ala Glu Leu 
    610                 615                 620                 
Phe Asp Ile Ile Asp Pro Ile Ala Ile Ala Glu Val Arg Glu Ala Leu 
625                 630                 635                 640 
Thr Arg Thr Leu Ala Thr Glu Leu Ala Asp Glu Leu Leu Ala Ile Tyr 
                645                 650                 655     
Asn Ala Asn Tyr Gln Ser Glu Tyr Arg Val Glu His Glu Asp Ile Ala 
            660                 665                 670         
Lys Arg Thr Leu Arg Asn Ala Cys Leu Arg Phe Leu Ala Phe Gly Glu 
        675                 680                 685             
Thr His Leu Ala Asp Val Leu Val Ser Lys Gln Phe His Glu Ala Asn 
    690                 695                 700                 
Asn Met Thr Asp Ala Leu Ala Ala Leu Ser Ala Ala Val Ala Ala Gln 
705                 710                 715                 720 
Leu Pro Cys Arg Asp Ala Leu Met Gln Glu Tyr Asp Asp Lys Trp His 
                725                 730                 735     
Gln Asn Gly Leu Val Met Asp Lys Trp Phe Ile Leu Gln Ala Thr Ser 
            740                 745                 750         
Pro Ala Ala Asn Val Leu Glu Thr Val Arg Gly Leu Leu Gln His Arg 
        755                 760                 765             
Ser Phe Thr Met Ser Asn Pro Asn Arg Ile Arg Ser Leu Ile Gly Ala 
    770                 775                 780                 
Phe Ala Gly Ser Asn Pro Ala Ala Phe His Ala Glu Asp Gly Ser Gly 
785                 790                 795                 800 
Tyr Leu Phe Leu Val Glu Met Leu Thr Asp Leu Asn Ser Arg Asn Pro 
                805                 810                 815     
Gln Val Ala Ser Arg Leu Ile Glu Pro Leu Ile Arg Leu Lys Arg Tyr 
            820                 825                 830         
Asp Ala Lys Arg Gln Glu Lys Met Arg Ala Ala Leu Glu Gln Leu Lys 
        835                 840                 845             
Gly Leu Glu Asn Leu Ser Gly Asp Leu Tyr Glu Lys Ile Thr Lys Ala 
    850                 855                 860                 
Leu Ala 
865     

<210> 33
<211> 185
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 3U7M; mutant; M65

<400> 33
Met Leu Thr Met Lys Asp Ile Ile Arg Asp Gly His Pro Thr Leu Arg 
1               5                   10                  15      
Gln Lys Ala Ala Glu Leu Glu Leu Pro Leu Thr Lys Glu Glu Lys Glu 
            20                  25                  30          
Thr Leu Ile Ala Met Arg Glu Phe Leu Val Asn Ser Gln Asp Glu Glu 
        35                  40                  45              
Ile Ala Lys Arg Tyr Gly Leu Arg Ser Leu Ala Met Leu Val Ala Pro 
    50                  55                  60                  
Leu Ile Asn Ile Ser Lys Arg Met Ile Val Val Leu Ile Pro Asp Asp 
65                  70                  75                  80  
Gly Ser Gly Lys Ser Tyr Asp Tyr Met Leu Val Asn Pro Lys Ile Val 
                85                  90                  95      
Ser His Ser Val Gln Glu Ala Tyr Leu Pro Thr Gly Phe Gln Cys Ile 
            100                 105                 110         
Ala Val Asp Asp Asn Val Ala Gly Leu Val His Leu His Asn Arg Ile 
        115                 120                 125             
Thr Ile Lys Ala Lys Asp Ile Glu Gly Asn Asp Ile Gln Leu Arg Leu 
    130                 135                 140                 
Lys Gly Leu Pro Ala Leu Ile Phe Gln His Ala Ile Asp His Leu Asn 
145                 150                 155                 160 
Gly Val Met Phe Tyr Asp His Ile Asp Lys Asp His Pro Leu Gln Pro 
                165                 170                 175     
His Thr Asp Ala Val Glu Val Leu Leu 
            180                 185 

<210> 34
<211> 257
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 1Z97; mutant; M65

<400> 34
Glu Trp Gly Tyr Ala Ser His Asn Gly Pro Asp His Trp His Glu Leu 
1               5                   10                  15      
Phe Pro Asn Ala Lys Gly Glu Asn Gln Ser Pro Ile Glu Leu His Thr 
            20                  25                  30          
Lys Asp Ile Arg His Asp Pro Ser Leu Gln Pro Trp Ser Val Ser Tyr 
        35                  40                  45              
Asp Gly Gly Ser Ala Lys Thr Ile Leu Asn Asp Gly Leu Thr Cys Leu 
    50                  55                  60                  
Val Val Phe Asp Asp Thr Tyr Asp Arg Ser Met Leu Arg Gly Gly Pro 
65                  70                  75                  80  
Leu Pro Gly Pro Tyr Arg Leu Leu Phe Phe His Leu His Trp Gly Ser 
                85                  90                  95      
Ser Asp Asp His Gly Ser Leu His Thr Val Asp Gly Val Lys Tyr Ala 
            100                 105                 110         
Ala Glu Leu His Leu Val His Trp Asn Pro Lys Tyr Asn Thr Tyr Lys 
        115                 120                 125             
Glu Ala Gln Lys Gln Arg Asp Gly Ile Ala Ile Ile Gly Ile Phe Leu 
    130                 135                 140                 
Lys Ile Gly His Glu Asn Gly Glu Phe Gln Ile Phe Leu Asp Ala Leu 
145                 150                 155                 160 
Asp Lys Ile Lys Thr Lys Gly Lys Glu Ala Pro Phe Thr Lys Phe Asp 
                165                 170                 175     
Pro Ser Ser Leu Phe Pro Ala Ser Arg Asp Tyr Trp Thr Tyr Gln Gly 
            180                 185                 190         
Ala Met Ala Val Pro Pro Leu Glu Glu Cys Val Val Trp Leu Leu Leu 
        195                 200                 205             
Lys Glu Pro Met Thr Val Ser Ser Asp Gln Met Ala Lys Leu Arg Ser 
    210                 215                 220                 
Leu Leu Ser Ser Ala Glu Asn Glu Pro Pro Val Pro Leu Val Ser Asn 
225                 230                 235                 240 
Trp Arg Pro Pro Gln Pro Ile Asn Asn Arg Val Val Arg Ala Ser Phe 
                245                 250                 255     
Lys 
    

<210> 35
<211> 185
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 3U7M; mutant; M72

<400> 35
Met Leu Thr Met Lys Asp Ile Ile Arg Asp Gly His Pro Thr Leu Arg 
1               5                   10                  15      
Gln Lys Ala Ala Glu Leu Glu Leu Pro Leu Thr Lys Glu Glu Lys Glu 
            20                  25                  30          
Thr Leu Ile Ala Met Arg Glu Phe Leu Val Asn Ser Leu Asp Glu Glu 
        35                  40                  45              
Ile Ala Lys Arg Tyr Gly Leu Arg Ser Ala Gly Met Leu Val Ala Pro 
    50                  55                  60                  
Met Ile Asn Ile Ser Lys Arg Met Ile Val Met Leu Ile Pro Asp Asp 
65                  70                  75                  80  
Gly Ser Gly Lys Ser Trp Asp Tyr Met Leu Val Asn Pro Lys Ile Val 
                85                  90                  95      
Ser His Ser Val Gln Glu Ala Tyr Leu Pro Thr Gly Glu Glu Cys Leu 
            100                 105                 110         
Ser Val Asp Asp Asn Val Ala Gly Leu Val His Arg His Asn Arg Ile 
        115                 120                 125             
Thr Ile Lys Ala Lys Asp Ile Glu Gly Asn Asp Ile Gln Leu Arg Leu 
    130                 135                 140                 
Lys Gly Leu Ala Ala Ile Ala Met Gln His Ala Ile Asp His Leu Asn 
145                 150                 155                 160 
Gly Val Met Phe Tyr Asp His Ile Asp Lys Asp His Pro Leu Gln Pro 
                165                 170                 175     
His Thr Asp Ala Val Glu Val Leu Leu 
            180                 185 

<210> 36
<211> 866
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 4Q4E; mutant; M72

<400> 36
Pro Gln Ala Lys Tyr Arg His Asp Tyr Arg Ala Pro Asp Tyr Gln Ile 
1               5                   10                  15      
Thr Asp Ile Asp Leu Thr Phe Asp Leu Asp Ala Gln Lys Thr Val Val 
            20                  25                  30          
Thr Ala Val Ser Gln Ala Val Arg His Gly Ala Ser Asp Ala Pro Leu 
        35                  40                  45              
Arg Leu Asn Gly Glu Asp Leu Lys Leu Val Ser Val His Ile Asn Asp 
    50                  55                  60                  
Glu Pro Trp Thr Ala Trp Lys Glu Glu Glu Gly Ala Leu Val Ile Ser 
65                  70                  75                  80  
Asn Leu Pro Glu Arg Phe Thr Leu Lys Ile Ile Asn Glu Ile Ser Pro 
                85                  90                  95      
Ala Ala Asn Thr Ala Leu Glu Gly Leu Tyr Gln Ser Gly Asp Ala Leu 
            100                 105                 110         
Cys Thr Gln Cys Val Ala Glu Gly Phe Arg His Ile Thr Tyr Tyr Leu 
        115                 120                 125             
Asp Arg Pro Asp Val Leu Ala Arg Phe Thr Thr Lys Ile Ile Ala Asp 
    130                 135                 140                 
Lys Ile Lys Tyr Pro Phe Leu Leu Ser Asn Gly Asn Arg Val Ala Gln 
145                 150                 155                 160 
Gly Glu Leu Glu Asn Gly Arg His Trp Val Gln Trp Gln Asp Pro Phe 
                165                 170                 175     
Pro Lys Pro Cys Tyr Leu Phe Ala Leu Val Ala Gly Asp Phe Asp Val 
            180                 185                 190         
Leu Arg Asp Thr Phe Thr Thr Arg Ser Gly Arg Glu Val Ala Leu Glu 
        195                 200                 205             
Leu Tyr Val Asp Arg Gly Asn Leu Asp Arg Ala Pro Trp Ala Met Thr 
    210                 215                 220                 
Ser Leu Lys Asn Ser Met Lys Trp Asp Glu Glu Arg Phe Gly Leu Glu 
225                 230                 235                 240 
Tyr Asp Leu Asp Ile Tyr Met Ile Val Ala Val Asp Phe Leu Asn Thr 
                245                 250                 255     
Gly Met Met Val Asn Lys Gly Leu Asn Ile Val His Ser Ile Ala Ile 
            260                 265                 270         
Leu Ala Arg Thr Asp Thr Ala Thr Asp Lys Asp Tyr Leu Asp Ile Glu 
        275                 280                 285             
Arg Leu Ile Gly His Ala Tyr Phe His Asn Trp Thr Gly Asn Arg Val 
    290                 295                 300                 
Thr Cys Arg Asp Trp Phe Gln Leu Ser Leu Ile Glu Gly Leu Thr Val 
305                 310                 315                 320 
Phe Arg Asp Gln Glu Phe Ser Ser Asp Leu Gly Ser Arg Ala Val Asn 
                325                 330                 335     
Arg Ile Asn Asn Val Arg Thr Met Arg Gly Leu Gln Phe Ala Glu Asp 
            340                 345                 350         
Ala Ser Pro Met Ala His Pro Ile Arg Pro Asp Met Val Ile Glu Met 
        355                 360                 365             
Asn Asn Phe Tyr Thr Leu Thr Val Leu Glu Lys Gly Ala Glu Val Ile 
    370                 375                 380                 
Arg Met Ile His Thr Leu Leu Gly Glu Glu Asn Phe Gln Lys Gly Met 
385                 390                 395                 400 
Gln Leu Tyr Phe Glu Arg His Asp Gly Ser Ala Ala Thr Cys Asp Asp 
                405                 410                 415     
Phe Val Gln Ala Met Glu Asp Ala Ser Asn Val Asp Leu Ser His Phe 
            420                 425                 430         
Arg Arg Trp Tyr Ser Gln Ser Gly Thr Pro Ile Val Thr Val Lys Asp 
        435                 440                 445             
Asp Tyr Asn Pro Glu Thr Glu Gln Tyr Thr Leu Thr Ile Ser Gln Arg 
    450                 455                 460                 
Thr Pro Ala Thr Pro Asp Gln Ala Glu Lys Gln Pro Leu His Ile Pro 
465                 470                 475                 480 
Phe Ala Ile Glu Leu Tyr Asp Asn Glu Gly Lys Val Ile Pro Leu Gln 
                485                 490                 495     
Lys Gly Gly His Pro Val Asn Ser Val Leu Asn Val Thr Gln Ala Glu 
            500                 505                 510         
Gln Thr Phe Val Phe Asp Asn Val Tyr Phe Gln Pro Val Pro Ala Leu 
        515                 520                 525             
Leu Cys Glu Phe Ser Ala Pro Val Lys Leu Glu Tyr Lys Trp Ser Asp 
    530                 535                 540                 
Gln Gln Leu Thr Phe Leu Met Arg His Ala Arg Asn Asp Phe Ser Arg 
545                 550                 555                 560 
Trp Asp Ala Ala Gln Ser Leu Leu Ala Thr Tyr Ile Lys Leu Asn Val 
                565                 570                 575     
Ala Arg His Gln Gln Gly Gln Pro Leu Ser Leu Pro Val His Val Ala 
            580                 585                 590         
Asp Ala Phe Arg Ala Val Leu Leu Asp Glu Lys Ile Asp Pro Ala Leu 
        595                 600                 605             
Ala Ala Glu Ile Leu Thr Leu Pro Ser Val Asn Glu Met Ala Glu Leu 
    610                 615                 620                 
Phe Asp Ile Ile Asp Pro Ile Ala Ile Ala Glu Val Arg Glu Ala Leu 
625                 630                 635                 640 
Thr Arg Thr Leu Ala Thr Glu Leu Ala Asp Glu Leu Leu Ala Ile Tyr 
                645                 650                 655     
Asn Ala Asn Tyr Gln Ser Glu Tyr Arg Val Glu His Glu Asp Ile Ala 
            660                 665                 670         
Lys Arg Thr Leu Arg Asn Ala Cys Leu Arg Phe Leu Ala Phe Gly Glu 
        675                 680                 685             
Thr His Leu Ala Asp Val Leu Val Ser Lys Gln Phe His Glu Ala Asn 
    690                 695                 700                 
Asn Met Thr Asp Ala Leu Ala Ala Leu Ser Ala Ala Val Ala Ala Gln 
705                 710                 715                 720 
Leu Pro Cys Arg Asp Ala Leu Met Gln Glu Tyr Asp Asp Lys Trp His 
                725                 730                 735     
Gln Asn Gly Leu Val Met Asp Lys Trp Phe Ile Leu Gln Ala Thr Ser 
            740                 745                 750         
Pro Ala Ala Asn Val Leu Glu Thr Val Arg Gly Leu Leu Gln His Arg 
        755                 760                 765             
Ser Phe Thr Met Ser Asn Pro Ile Arg Ile Leu Ser Leu Ile Gly Ala 
    770                 775                 780                 
Phe Ala Gly Ser Asn Pro Ala Ala Phe His Ala Glu Asp Gly Ser Gly 
785                 790                 795                 800 
Tyr Leu Phe Leu Val Glu Met Leu Thr Asp Leu Asn Ser Arg Asn Pro 
                805                 810                 815     
Gln Val Ala Ser Arg Leu Ile Glu Pro Leu Ile Arg Leu Lys Arg Tyr 
            820                 825                 830         
Asp Ala Lys Arg Gln Glu Lys Met Arg Ala Ala Leu Glu Gln Leu Lys 
        835                 840                 845             
Gly Leu Glu Asn Leu Ser Gly Asp Leu Tyr Glu Lys Ile Thr Lys Ala 
    850                 855                 860                 
Leu Ala 
865     

<210> 37
<211> 478
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 1LML; mutant; M72

<400> 37
Val Val Arg Asp Val Asn Trp Gly Ala Leu Arg Ile Ala Val Ser Thr 
1               5                   10                  15      
Glu Asp Leu Thr Asp Pro Ala Tyr His Cys Ala Arg Val Gly Gln His 
            20                  25                  30          
Val Lys Asp His Ala Gly Ala Ile Val Thr Cys Thr Ala Glu Asp Ile 
        35                  40                  45              
Leu Thr Asn Glu Lys Arg Asp Ile Leu Val Lys His Leu Ile Pro Gln 
    50                  55                  60                  
Ala Val Gln Leu His Thr Glu Arg Leu Lys Val Gln Gln Val Gln Gly 
65                  70                  75                  80  
Lys Trp Lys Val Thr Asp Met Val Gly Asp Ile Cys Gly Asp Phe Lys 
                85                  90                  95      
Val Pro Gln Ala His Ile Thr Glu Gly Phe Ser Asn Thr Asp Phe Val 
            100                 105                 110         
Met Tyr Val Ala Ser Val Pro Ser His Glu Gly Ile Leu Ala Trp Ala 
        115                 120                 125             
Thr Thr Cys Gln Thr Phe Ser Asp Gly His Pro Ala Val Gly Val Ile 
    130                 135                 140                 
Asn Ile Pro Ala Ala Asn Ile Ala Ser Arg Tyr Asp Gln Leu Val Thr 
145                 150                 155                 160 
Arg Val Val Thr His Ala Met Ala His Ala Leu Gly Phe Ser Gly Pro 
                165                 170                 175     
Phe Phe Glu Asp Ala Arg Ile Val Ala Asn Val Pro Asn Val Arg Gly 
            180                 185                 190         
Lys Asn Phe Asp Val Pro Val Ile Asn Ser Ser Thr Ala Val Ala Lys 
        195                 200                 205             
Ala Arg Glu Gln Tyr Gly Cys Asp Thr Leu Glu Tyr Leu Glu Val Glu 
    210                 215                 220                 
Asp Gln Gly Gly Ala Lys Ile Ala Gly Ser His Ile Lys Met Arg Asn 
225                 230                 235                 240 
Ala Gln Asp Glu Leu Met Ala Pro Lys Ala Ala Ala Gly Tyr Tyr Thr 
                245                 250                 255     
Ala Leu Thr Met Ala Ile Phe Gln Asp Leu Gly Phe Tyr Gln Ala Asp 
            260                 265                 270         
Phe Ser Lys Ala Glu Val Met Pro Trp Gly Gln Asn Ala Gly Cys Ala 
        275                 280                 285             
Phe Leu Thr Asn Lys Cys Met Glu Gln Ser Val Thr Gln Trp Pro Ala 
    290                 295                 300                 
Met Phe Cys Asn Glu Ser Glu Asp Ala Ile Arg Cys Pro Thr Ser Arg 
305                 310                 315                 320 
Leu Ser Leu Gly Ala Cys Gly Val Thr Arg His Pro Gly Leu Pro Pro 
                325                 330                 335     
Tyr Trp Gln Tyr Phe Thr Asp Pro Ser Leu Ala Gly Val Ser Ala Leu 
            340                 345                 350         
Met Asp Tyr Cys Pro Val Val Val Pro Tyr Ser Asp Gly Ser Cys Thr 
        355                 360                 365             
Gln Arg Ala Ser Glu Ala His Ala Ser Leu Leu Pro Phe Asn Val Phe 
    370                 375                 380                 
Ser Asp Ala Ala Arg Cys Ile Asp Gly Ala Phe Arg Pro Lys Ala Thr 
385                 390                 395                 400 
Asp Gly Ile Val Lys Ser Tyr Ala Gly Leu Cys Ala Asn Val Gln Cys 
                405                 410                 415     
Asp Thr Ala Thr Arg Thr Tyr Ser Val Gln Val His Gly Ser Asn Asp 
            420                 425                 430         
Tyr Thr Asn Cys Thr Pro Gly Leu Arg Val Glu Leu Ser Thr Val Ser 
        435                 440                 445             
Asn Ala Phe Glu Gly Gly Gly Tyr Ile Thr Cys Pro Pro Tyr Val Glu 
    450                 455                 460                 
Val Cys Gln Gly Asn Val Gln Ala Ala Lys Asp Gly Gly Asn 
465                 470                 475             

<210> 38
<211> 860
<212> PRT
<213> Artificial Sequence


<220> 
<223> 3UJZ; mutant, M72

<400> 38
Asn Ser Ala Ile Tyr Phe Asn Thr Ser Gln Pro Ile Asn Asp Leu Gln 
1               5                   10                  15      
Gly Ser Leu Ala Ala Glu Val Lys Phe Ala Gln Ser Gln Ile Leu Pro 
            20                  25                  30          
Ala His Pro Lys Glu Gly Asp Ser Gln Pro His Leu Thr Ser Leu Arg 
        35                  40                  45              
Lys Ser Leu Leu Leu Val Arg Pro Val Lys Ala Asp Asp Lys Thr Pro 
    50                  55                  60                  
Val Gln Val Glu Ala Arg Asp Asp Asn Asn Lys Ile Leu Gly Thr Leu 
65                  70                  75                  80  
Thr Leu Tyr Pro Pro Ser Ser Leu Pro Asp Thr Ile Tyr His Leu Asp 
                85                  90                  95      
Gly Val Pro Glu Gly Gly Ile Asp Phe Thr Pro His Asn Gly Thr Lys 
            100                 105                 110         
Lys Ile Ile Asn Thr Val Ala Glu Val Asn Lys Leu Ser Asp Ala Ser 
        115                 120                 125             
Gly Ser Ser Ile His Ser His Leu Thr Asn Asn Ala Leu Val Glu Ile 
    130                 135                 140                 
His Thr Ala Asn Gly Arg Trp Val Arg Asp Ile Tyr Leu Pro Gln Gly 
145                 150                 155                 160 
Pro Asp Leu Glu Gly Lys Met Val Arg Phe Val Ser Ser Ala Gly Tyr 
                165                 170                 175     
Ser Ser Thr Val Phe Tyr Gly Asp Arg Lys Val Thr Leu Ser Val Gly 
            180                 185                 190         
Asn Thr Leu Leu Phe Lys Tyr Val Asn Gly Gln Trp Phe Arg Ser Gly 
        195                 200                 205             
Glu Leu Glu Asn Asn Arg Ile Thr Tyr Ala Gln His Ile Trp Ser Ala 
    210                 215                 220                 
Glu Leu Pro Ala His Trp Ile Val Pro Gly Leu Asn Leu Val Ile Lys 
225                 230                 235                 240 
Gln Gly Asn Leu Ser Gly Arg Leu Asn Asp Ile Lys Ile Gly Ala Pro 
                245                 250                 255     
Gly Glu Leu Leu Leu His Thr Ile Asp Ile Gly Met Leu Thr Thr Pro 
            260                 265                 270         
Arg Asp Arg Phe Asp Phe Ala Ala Asp Ala Ala Ala His Arg Glu Tyr 
        275                 280                 285             
Phe Gln Thr Ile Pro Val Ser Arg Met Ile Val Asn Asn Tyr Ala Pro 
    290                 295                 300                 
Leu His Leu Lys Glu Val Met Leu Pro Thr Gly Glu Leu Leu Thr Asp 
305                 310                 315                 320 
Met Asp Pro Gly Asn Gly Gly Glu Thr Ser Gly Thr Met Arg Gln Arg 
                325                 330                 335     
Ile Gly Lys Glu Leu Val Ser His Gly Ile Asp Asn Ala Asn Tyr Gly 
            340                 345                 350         
Leu Asn Ser Thr Ala Gly Leu Gly Glu Asn Ser His Pro Tyr Val Val 
        355                 360                 365             
Ala Gln Leu Ala Ala His Asn Ser Arg Gly Asn Tyr Ala Asn Gly Ile 
    370                 375                 380                 
Gln Val His Gly Lys Ser Gly Gly Gly Gly Ile Val Thr Leu Asp Ser 
385                 390                 395                 400 
Thr Leu Gly Asn Glu Phe Ser His Ala Val Gly His Asn Tyr Gly Leu 
                405                 410                 415     
Gly His Tyr Val Asp Gly Phe Lys Gly Ser Val His Arg Ser Ala Glu 
            420                 425                 430         
Asn Asn Asn Ser Thr Trp Gly Trp Asp Gly Asp Lys Lys Arg Phe Ile 
        435                 440                 445             
Pro Asn Phe Tyr Pro Ser Gln Thr Asn Glu Lys Ser Cys Leu Asn Asn 
    450                 455                 460                 
Gln Cys Gln Glu Pro Phe Asp Gly His Lys Phe Gly Phe Asp Ala Met 
465                 470                 475                 480 
Ala Gly Gly Ser Pro Phe Ser Ala Ala Asn Arg Phe Thr Met Tyr Thr 
                485                 490                 495     
Pro Asn Ser Ser Ala Ile Ile Gln Arg Phe Phe Glu Asn Lys Ala Val 
            500                 505                 510         
Phe Asp Ser Arg Ser Ser Thr Gly Phe Ser Lys Trp Asn Ala Asp Thr 
        515                 520                 525             
Gln Glu Met Glu Pro Tyr Glu His Thr Ile Asp Arg Ala Glu Gln Ile 
    530                 535                 540                 
Thr Ala Ser Val Asn Glu Leu Ser Glu Ser Lys Met Ala Glu Leu Met 
545                 550                 555                 560 
Ala Glu Tyr Ala Val Val Lys Val His Met Trp Asn Gly Asn Trp Thr 
                565                 570                 575     
Arg Asn Ile Tyr Ile Pro Thr Ala Ser Ala Asp Asn Arg Gly Ser Ile 
            580                 585                 590         
Leu Thr Ile Asn His Glu Ala Gly Tyr Asn Ser Tyr Leu Phe Ile Asn 
        595                 600                 605             
Gly Asp Glu Lys Val Val Ser Gln Gly Tyr Lys Lys Ser Phe Val Ser 
    610                 615                 620                 
Asp Gly Gln Phe Trp Lys Glu Arg Asp Val Val Asp Thr Arg Glu Ala 
625                 630                 635                 640 
Arg Lys Pro Glu Gln Phe Gly Val Pro Val Thr Thr Leu Val Gly Tyr 
                645                 650                 655     
Tyr Asp Pro Glu Gly Thr Leu Ser Ser Tyr Ile Tyr Pro Ala Met Tyr 
            660                 665                 670         
Gly Ala Tyr Gly Phe Thr Tyr Ser Asp Asp Ser Gln Asn Leu Ser Asp 
        675                 680                 685             
Asn Asp Cys Gln Leu Gln Val Asp Thr Lys Glu Gly Gln Leu Arg Phe 
    690                 695                 700                 
Arg Leu Ala Asn His Arg Ala Asn Asn Thr Val Met Asn Lys Phe His 
705                 710                 715                 720 
Ile Asn Val Pro Thr Glu Ser Gln Pro Thr Gln Ala Thr Leu Val Cys 
                725                 730                 735     
Asn Asn Lys Ile Leu Asp Thr Lys Ser Leu Thr Pro Ala Pro Glu Gly 
            740                 745                 750         
Leu Thr Tyr Thr Val Asn Gly Gln Ala Leu Pro Ala Lys Glu Asn Glu 
        755                 760                 765             
Gly Cys Ile Val Ser Val Asn Ser Gly Lys Arg Tyr Cys Leu Pro Val 
    770                 775                 780                 
Gly Gln Arg Ser Gly Tyr Ser Leu Pro Asp Trp Ile Val Gly Gln Glu 
785                 790                 795                 800 
Val Tyr Val Asp Ser Gly Ala Lys Ala Lys Val Leu Leu Ser Asp Trp 
                805                 810                 815     
Asp Asn Leu Ser Tyr Asn Arg Ile Gly Glu Phe Val Gly Asn Val Asn 
            820                 825                 830         
Pro Ala Asp Met Lys Lys Val Lys Ala Trp Asn Gly Gln Tyr Leu Asp 
        835                 840                 845             
Phe Ser Lys Pro Arg Ser Met Arg Val Val Tyr Lys 
    850                 855                 860 

<210> 39
<211> 201
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 1IAG; M83

<400> 39
Asn Leu Pro Gln Arg Tyr Ile Glu Leu Val Val Val Ala Asp Arg Arg 
1               5                   10                  15      
Val Phe Met Lys Tyr Asn Ser Asp Leu Asn Ile Ile Arg Thr Arg Val 
            20                  25                  30          
His Glu Ile Val Asn Ile Ile Asn Lys Phe Tyr Arg Ser Leu Asn Ile 
        35                  40                  45              
Arg Val Ser Leu Thr Asp Leu Glu Ile Trp Ser Gly Gln Asp Phe Ile 
    50                  55                  60                  
Thr Ile Gln Ser Ser Ser Ser Asn Thr Leu Asn Ser Phe Gly Glu Trp 
65                  70                  75                  80  
Arg Glu Arg Val Leu Leu Ile Trp Lys Arg His Asp Asn Ala Gln Leu 
                85                  90                  95      
Leu Thr Ala Ile Asn Phe Glu Gly Lys Ile Ile Leu Leu Ala Tyr Thr 
            100                 105                 110         
Ser Ser Met Cys Asn Pro Arg Ser Ser Val Gly Ile Val Lys Asp His 
        115                 120                 125             
Ser Pro Ile Asn Leu Leu Val Ala Val Thr Met Ala His Leu Leu Gly 
    130                 135                 140                 
His Asn Leu Gly Met Glu His Asp Gly Pro Asp Cys Leu Arg Gly Ala 
145                 150                 155                 160 
Ser Leu Cys Ile Met Leu Pro Glu Leu Thr Pro Gly Arg Ser Tyr Glu 
                165                 170                 175     
Phe Ser Asp Asp Ser Met Gly Tyr Tyr Gln Lys Phe Leu Asn Gln Tyr 
            180                 185                 190         
Lys Pro Gln Cys Ile Leu Asn Lys Pro 
        195                 200     

<210> 40
<211> 866
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 4Q4E; mutant; M83

<400> 40
Pro Gln Ala Lys Tyr Arg His Asp Tyr Arg Ala Pro Asp Tyr Gln Ile 
1               5                   10                  15      
Thr Asp Ile Asp Leu Thr Phe Asp Leu Asp Ala Gln Lys Thr Val Val 
            20                  25                  30          
Thr Ala Val Ser Gln Ala Val Arg His Gly Ala Ser Asp Ala Pro Leu 
        35                  40                  45              
Arg Leu Asn Gly Glu Asp Leu Lys Leu Val Ser Val His Ile Asn Asp 
    50                  55                  60                  
Glu Pro Trp Thr Ala Trp Lys Glu Glu Glu Gly Ala Leu Val Ile Ser 
65                  70                  75                  80  
Asn Leu Pro Glu Arg Phe Thr Leu Lys Ile Ile Asn Glu Ile Ser Pro 
                85                  90                  95      
Ala Ala Asn Thr Ala Leu Glu Gly Leu Tyr Gln Ser Gly Asp Ala Leu 
            100                 105                 110         
Cys Thr Gln Cys Met Ala Glu Gly Phe Arg His Ile Thr Tyr Tyr Leu 
        115                 120                 125             
Asp Arg Pro Asp Val Leu Ala Arg Phe Thr Thr Lys Ile Ile Ala Asp 
    130                 135                 140                 
Lys Ile Lys Tyr Pro Phe Leu Leu Ser Asn Gly Asn Arg Val Ala Gln 
145                 150                 155                 160 
Gly Glu Leu Glu Asn Gly Arg His Trp Val Gln Trp Gln Asp Pro Phe 
                165                 170                 175     
Pro Lys Pro Cys Tyr Leu Phe Ala Leu Val Ala Gly Asp Phe Asp Val 
            180                 185                 190         
Leu Arg Asp Thr Phe Thr Thr Arg Ser Gly Arg Glu Val Ala Leu Glu 
        195                 200                 205             
Leu Tyr Val Asp Arg Gly Asn Leu Asp Arg Ala Pro Trp Ala Met Thr 
    210                 215                 220                 
Ser Leu Lys Asn Ser Met Lys Trp Asp Glu Glu Arg Phe Gly Leu Glu 
225                 230                 235                 240 
Tyr Asp Leu Asp Ile Tyr Met Ile Val Ala Val Asp Phe Phe Asn Thr 
                245                 250                 255     
Leu Leu Ile Pro Asn Lys Gly Leu Asn Ile Phe Asn Ser Lys Leu Val 
            260                 265                 270         
Leu Ala Arg Thr Asp Thr Ala Thr Asp Glu Asp Tyr Leu Arg Ile Glu 
        275                 280                 285             
Ala Ala Ile Gly His Ile Tyr Phe His Asn Trp Thr Gly Asn Arg Val 
    290                 295                 300                 
Thr Cys Arg Asp Trp Phe Gln Leu Ser Leu Lys Glu Gly Leu Thr Val 
305                 310                 315                 320 
Phe Arg Asp Gln Glu Phe Ser Ser Asp Leu Gly Ser Arg Ala Val Asn 
                325                 330                 335     
Arg Ile Asn Asn Val Arg Ile Met Arg Gly Leu Gln Phe Ala Glu Asp 
            340                 345                 350         
Ala Ser Pro Met Ala His Pro Ile Arg Pro Asp Met Val Ile Glu Met 
        355                 360                 365             
Asn Asn Phe Tyr Thr Leu Thr Val Leu Tyr Lys Gly Ala Glu Val Ile 
    370                 375                 380                 
Arg Met Ile His Thr Leu Leu Gly Glu Glu Asn Phe Gln Lys Gly Met 
385                 390                 395                 400 
Gln Leu Tyr Phe Glu Arg His Asp Gly Ser Ala Ala Thr Cys Asp Asp 
                405                 410                 415     
Phe Val Gln Ala Met Glu Asp Ala Ser Asn Val Asp Leu Ser His Phe 
            420                 425                 430         
Arg Arg Trp Tyr Ser Gln Ser Gly Thr Pro Ile Val Thr Val Lys Asp 
        435                 440                 445             
Asp Tyr Asn Pro Glu Thr Glu Gln Tyr Thr Leu Thr Ile Ser Gln Arg 
    450                 455                 460                 
Thr Pro Ala Thr Pro Asp Gln Ala Glu Lys Gln Pro Leu His Ile Pro 
465                 470                 475                 480 
Phe Ala Ile Glu Leu Tyr Asp Asn Glu Gly Lys Val Ile Pro Leu Gln 
                485                 490                 495     
Lys Gly Gly His Pro Val Asn Ser Val Leu Asn Val Thr Gln Ala Glu 
            500                 505                 510         
Gln Thr Phe Val Phe Asp Asn Val Tyr Phe Gln Pro Val Pro Ala Leu 
        515                 520                 525             
Leu Cys Glu Phe Ser Ala Pro Val Lys Leu Glu Tyr Lys Trp Ser Asp 
    530                 535                 540                 
Gln Gln Leu Thr Phe Leu Met Arg His Ala Arg Asn Asp Phe Ser Arg 
545                 550                 555                 560 
Trp Asp Ala Ala Gln Ser Leu Leu Ala Thr Tyr Ile Lys Leu Asn Val 
                565                 570                 575     
Ala Arg His Gln Gln Gly Gln Pro Leu Ser Leu Pro Val His Val Ala 
            580                 585                 590         
Asp Ala Phe Arg Ala Val Leu Leu Asp Glu Lys Ile Asp Pro Ala Leu 
        595                 600                 605             
Ala Ala Glu Ile Leu Thr Leu Pro Ser Val Asn Glu Met Ala Glu Leu 
    610                 615                 620                 
Phe Asp Ile Ile Asp Pro Ile Ala Ile Ala Glu Val Arg Glu Ala Leu 
625                 630                 635                 640 
Thr Arg Thr Leu Ala Thr Glu Leu Ala Asp Glu Leu Leu Ala Ile Tyr 
                645                 650                 655     
Asn Ala Asn Tyr Gln Ser Glu Tyr Arg Val Glu His Glu Asp Ile Ala 
            660                 665                 670         
Lys Arg Thr Leu Arg Asn Ala Cys Leu Arg Phe Leu Ala Phe Gly Glu 
        675                 680                 685             
Thr His Leu Ala Asp Val Leu Val Ser Lys Gln Phe His Glu Ala Asn 
    690                 695                 700                 
Asn Met Thr Asp Ala Leu Ala Ala Leu Ser Ala Ala Val Ala Ala Gln 
705                 710                 715                 720 
Leu Pro Cys Arg Asp Ala Leu Met Gln Glu Tyr Asp Asp Lys Trp His 
                725                 730                 735     
Gln Asn Gly Leu Val Met Asp Lys Trp Phe Ile Leu Gln Ala Thr Ser 
            740                 745                 750         
Pro Ala Ala Asn Val Leu Glu Thr Val Arg Gly Leu Leu Gln His Arg 
        755                 760                 765             
Ser Phe Thr Met Ser Asn Pro Asn Arg Ile Arg Ser Leu Ile Gly Ala 
    770                 775                 780                 
Phe Ala Gly Ser Asn Pro Ala Ala Phe His Ala Glu Asp Gly Ser Gly 
785                 790                 795                 800 
Tyr Leu Phe Leu Val Glu Met Leu Thr Asp Leu Asn Ser Arg Asn Pro 
                805                 810                 815     
Gln Val Ala Ser Arg Leu Ile Glu Pro Leu Ile Arg Leu Lys Arg Tyr 
            820                 825                 830         
Asp Ala Lys Arg Gln Glu Lys Met Arg Ala Ala Leu Glu Gln Leu Lys 
        835                 840                 845             
Gly Leu Glu Asn Leu Ser Gly Asp Leu Tyr Glu Lys Ile Thr Lys Ala 
    850                 855                 860                 
Leu Ala 
865     

<210> 41
<211> 478
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 1LML; mutant; M83

<400> 41
Val Val Arg Asp Val Asn Trp Gly Ala Leu Arg Ile Ala Val Ser Thr 
1               5                   10                  15      
Glu Asp Leu Thr Asp Pro Ala Tyr His Cys Ala Arg Val Gly Gln His 
            20                  25                  30          
Val Lys Asp His Ala Gly Ala Ile Val Thr Cys Thr Ala Glu Asp Ile 
        35                  40                  45              
Leu Thr Asn Glu Lys Arg Asp Ile Leu Val Lys His Leu Ile Pro Gln 
    50                  55                  60                  
Ala Val Gln Leu His Thr Glu Arg Leu Lys Val Gln Gln Val Gln Gly 
65                  70                  75                  80  
Lys Trp Lys Val Thr Asp Met Val Gly Asp Ile Cys Gly Asp Phe Lys 
                85                  90                  95      
Val Pro Gln Ala His Ile Thr Glu Gly Phe Ser Asn Thr Asp Phe Val 
            100                 105                 110         
Met Tyr Val Ala Ser Val Pro Ser Glu Glu Gly Val Leu Ala Trp Ala 
        115                 120                 125             
Thr Thr Cys Gln Thr Phe Ser Asp Gly His Pro Ala Val Gly Val Ile 
    130                 135                 140                 
Asn Ile Pro Ala Ala Asn Ile Ala Ser Arg Tyr Asp Gln Leu Val Thr 
145                 150                 155                 160 
Arg Val Val Thr His Ala Met Ala His Ala Leu Gly Phe Ser Gly Pro 
                165                 170                 175     
Phe Phe Glu Asp Ala Arg Ile Val Ala Asn Val Pro Asn Val Arg Gly 
            180                 185                 190         
Lys Asn Phe Asp Val Pro Val Ile Asn Ser Ser Thr Ala Val Ala Lys 
        195                 200                 205             
Ala Arg Glu Gln Tyr Gly Cys Asp Thr Leu Glu Tyr Leu Glu Val Glu 
    210                 215                 220                 
Asp Gln Ala Gly Ala Lys Ala Ala Gly Ser His Ile Lys Met Arg Asn 
225                 230                 235                 240 
Ala Gln Asp Glu Leu Met Ala Pro Ala Ala Ala Ala Gly Tyr Tyr Thr 
                245                 250                 255     
Ala Leu Thr Met Ala Ile Phe Gln Asp Leu Gly Phe Tyr Gln Ala Asp 
            260                 265                 270         
Phe Ser Lys Ala Glu Val Met Pro Trp Gly Gln Asn Ala Gly Cys Ala 
        275                 280                 285             
Phe Leu Thr Asn Lys Cys Met Glu Gln Ser Val Thr Gln Trp Pro Ala 
    290                 295                 300                 
Met Phe Cys Asn Glu Ser Glu Asp Ala Ile Arg Cys Pro Thr Ser Arg 
305                 310                 315                 320 
Leu Ser Leu Gly Ala Cys Gly Val Thr Arg His Pro Gly Leu Pro Pro 
                325                 330                 335     
Tyr Trp Gln Tyr Phe Thr Asp Pro Ser Leu Ala Gly Val Ser Ala Arg 
            340                 345                 350         
Met Asp Tyr Cys Pro Val Val Val Pro Tyr Ser Asp Gly Ser Cys Thr 
        355                 360                 365             
Gln Arg Ala Ser Glu Ala His Ala Ser Leu Leu Pro Phe Asn Val Phe 
    370                 375                 380                 
Ser Asp Ala Ala Arg Cys Ile Asp Gly Ala Phe Arg Pro Lys Ala Thr 
385                 390                 395                 400 
Asp Gly Ile Val Lys Ser Tyr Ala Gly Leu Cys Ala Asn Val Gln Cys 
                405                 410                 415     
Asp Thr Ala Thr Arg Thr Tyr Ser Val Gln Val His Gly Ser Asn Asp 
            420                 425                 430         
Tyr Thr Asn Cys Thr Pro Gly Leu Arg Val Glu Leu Ser Thr Val Ser 
        435                 440                 445             
Asn Ala Phe Glu Gly Gly Gly Tyr Ile Thr Cys Pro Pro Tyr Val Glu 
    450                 455                 460                 
Val Cys Gln Gly Asn Val Gln Ala Ala Lys Asp Gly Gly Asn 
465                 470                 475             

<210> 42
<211> 478
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 1LML; mutant; M86

<400> 42
Val Val Arg Asp Val Asn Trp Gly Ala Leu Arg Ile Ala Val Ser Thr 
1               5                   10                  15      
Glu Asp Leu Thr Asp Pro Ala Tyr His Cys Ala Arg Val Gly Gln His 
            20                  25                  30          
Val Lys Asp His Ala Gly Ala Ile Val Thr Cys Thr Ala Glu Asp Ile 
        35                  40                  45              
Leu Thr Asn Glu Lys Arg Asp Ile Leu Val Lys His Leu Ile Pro Gln 
    50                  55                  60                  
Ala Val Gln Leu His Thr Glu Arg Leu Lys Val Gln Gln Val Gln Gly 
65                  70                  75                  80  
Lys Trp Lys Val Thr Asp Met Val Gly Asp Ile Cys Gly Asp Phe Lys 
                85                  90                  95      
Val Pro Gln Ala His Ile Thr Glu Gly Phe Ser Asn Thr Asp Phe Val 
            100                 105                 110         
Met Tyr Val Ala Ser Val Pro Ser His Glu Gly Val Leu Ala Trp Ala 
        115                 120                 125             
Thr Thr Cys Gln Thr Phe Ser Asp Gly His Pro Ala Val Gly Val Ile 
    130                 135                 140                 
Asn Ile Pro Ala Ala Asn Ile Ala Ser Arg Tyr Asp Gln Leu Val Thr 
145                 150                 155                 160 
Arg Val Val Thr His Val Met Ala His Ala Leu Gly Phe Ser Gly Pro 
                165                 170                 175     
Phe Phe Glu Asp Ala Arg Ile Val Ala Asn Val Pro Asn Val Arg Gly 
            180                 185                 190         
Lys Asn Phe Asp Val Pro Val Ile Asn Ser Ser Thr Ala Val Ala Lys 
        195                 200                 205             
Ala Arg Glu Gln Tyr Gly Cys Asp Thr Leu Glu Tyr Leu Glu Val Glu 
    210                 215                 220                 
Asp Gln Gly Gly Arg Gly Val Ala Gly Ser His Ile Lys Met Arg Asn 
225                 230                 235                 240 
Ala Gln Asp Glu Leu Met Ala Pro Asp Ala Ala Ala Gly Tyr Tyr Thr 
                245                 250                 255     
Ala Leu Thr Met Ala Ile Phe Gln Asp Leu Gly Phe Tyr Gln Ala Asp 
            260                 265                 270         
Phe Ser Lys Ala Glu Val Met Pro Trp Gly Gln Asn Ala Gly Cys Ala 
        275                 280                 285             
Phe Leu Thr Asn Lys Cys Met Glu Gln Ser Val Thr Gln Trp Pro Ala 
    290                 295                 300                 
Met Phe Cys Asn Glu Ser Glu Asp Ala Ile Arg Cys Pro Thr Ser Arg 
305                 310                 315                 320 
Leu Ser Leu Gly Ala Cys Gly Val Thr Arg His Pro Gly Leu Pro Pro 
                325                 330                 335     
Tyr Trp Gln Tyr Phe Thr Asp Pro Ser Leu Ala Gly Val Ser Ala Leu 
            340                 345                 350         
Met Asp Tyr Cys Pro Val Val Val Pro Tyr Ser Asp Gly Ser Cys Thr 
        355                 360                 365             
Gln Arg Ala Ser Glu Ala His Ala Ser Leu Leu Pro Phe Asn Val Phe 
    370                 375                 380                 
Ser Asp Ala Ala Arg Cys Ile Asp Gly Ala Phe Arg Pro Lys Ala Thr 
385                 390                 395                 400 
Asp Gly Ile Val Lys Ser Tyr Ala Gly Leu Cys Ala Asn Val Gln Cys 
                405                 410                 415     
Asp Thr Ala Thr Arg Thr Tyr Ser Val Gln Val His Gly Ser Asn Asp 
            420                 425                 430         
Tyr Thr Asn Cys Thr Pro Gly Leu Arg Val Glu Leu Ser Thr Val Ser 
        435                 440                 445             
Asn Ala Phe Glu Gly Gly Gly Tyr Ile Thr Cys Pro Pro Tyr Val Glu 
    450                 455                 460                 
Val Cys Gln Gly Asn Val Gln Ala Ala Lys Asp Gly Gly Asn 
465                 470                 475             

<210> 43
<211> 724
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 5E3C; mutant; M86

<400> 43
Asp Thr Gln Tyr Ile Leu Pro Asn Asp Ile Gly Val Ser Ser Leu Asp 
1               5                   10                  15      
Ser Arg Glu Ala Phe Arg Leu Leu Ser Pro Thr Glu Arg Leu Tyr Ala 
            20                  25                  30          
Tyr His Leu Ser Arg Ala Ala Trp Tyr Gly Gly Leu Ala Val Leu Leu 
        35                  40                  45              
Gln Thr Ser Pro Glu Ala Pro Tyr Ile Tyr Ala Leu Leu Ser Arg Leu 
    50                  55                  60                  
Phe Arg Ala Gln Asp Pro Asp Gln Leu Arg Gln His Ala Leu Ala Glu 
65                  70                  75                  80  
Gly Leu Thr Glu Glu Glu Tyr Gln Ala Phe Leu Val Tyr Ala Ala Gly 
                85                  90                  95      
Val Tyr Ser Asn Met Gly Asn Tyr Lys Ala Trp Gly Asp Thr Lys Phe 
            100                 105                 110         
Val Pro Asn Leu Pro Lys Glu Lys Leu Glu Arg Val Ile Leu Gly Ser 
        115                 120                 125             
Glu Ala Ala Gln Gln His Pro Glu Glu Val Arg Gly Leu Trp Gln Thr 
    130                 135                 140                 
Cys Gly Glu Leu Met Phe Ser Leu Glu Pro Arg Leu Arg His Leu Gly 
145                 150                 155                 160 
Leu Gly Lys Glu Gly Ile Thr Thr Tyr Phe Ser Gly Asn Cys Thr Met 
                165                 170                 175     
Glu Asp Ala Lys Leu Ala Gln Asp Phe Leu Asp Ser Gln Asn Leu Ser 
            180                 185                 190         
Ala Tyr Asn Thr Arg Leu Phe Lys Glu Val Asp Gly Cys Gly Lys Pro 
        195                 200                 205             
Tyr Tyr Glu Val Arg Leu Ala Ser Val Leu Gly Ser Glu Pro Ser Leu 
    210                 215                 220                 
Asp Ser Glu Val Thr Ser Lys Leu Lys Ser Tyr Glu Phe Arg Gly Ser 
225                 230                 235                 240 
Pro Phe Gln Val Thr Arg Gly Asp Tyr Ala Pro Ile Leu Gln Lys Val 
                245                 250                 255     
Val Glu Gln Leu Glu Lys Ala Lys Ala Tyr Ala Ala Asn Ser His Gln 
            260                 265                 270         
Gly Gln Met Leu Ala Gln Tyr Ile Glu Ser Phe Thr Gln Gly Ser Ile 
        275                 280                 285             
Glu Ala His Lys Arg Gly Ser Arg Phe Trp Ile Gln Asp Lys Gly Pro 
    290                 295                 300                 
Ile Val Glu Ser Tyr Ile Gly Phe Ile Trp Ser Val Phe Asp Pro Phe 
305                 310                 315                 320 
Gly Ser Arg Gly Met Phe Phe Gly Phe Val Ala Val Val Asn Lys Ala 
                325                 330                 335     
Met Ser Ala Lys Phe Glu Arg Leu Val Ala Ser Ala Glu Gln Leu Leu 
            340                 345                 350         
Lys Glu Leu Pro Trp Pro Pro Thr Phe Glu Lys Asp Lys Phe Leu Thr 
        355                 360                 365             
Pro Asp Phe Thr Ser Leu Asp Val Leu Thr Met Ala Gly Leu Gly Ile 
    370                 375                 380                 
Pro Leu Met Met Val Ile Pro Asn Tyr Asp Asp Leu Arg Gln Thr Glu 
385                 390                 395                 400 
Gly Phe Lys Asn Val Ser Leu Gly Asn Val Leu Ala Val Ala Tyr Ala 
                405                 410                 415     
Thr Gln Arg Glu Lys Leu Thr Phe Leu Glu Glu Asp Asp Lys Asp Leu 
            420                 425                 430         
Tyr Ile Leu Trp Lys Gly Pro Ser Phe Asp Val Gln Val Gly Leu His 
        435                 440                 445             
Ala Leu Leu Gly His Gly Ser Gly Lys Leu Phe Val Gln Asp Glu Lys 
    450                 455                 460                 
Gly Ala Phe Asn Phe Asp Gln Glu Thr Val Ile Asn Pro Glu Thr Gly 
465                 470                 475                 480 
Glu Gln Ile Gln Ser Trp Tyr Arg Cys Gly Glu Thr Trp Asp Ser Lys 
                485                 490                 495     
Phe Ser Thr Ile Ala Ser Ser Tyr Glu Glu Cys Arg Ala Glu Ser Val 
            500                 505                 510         
Gly Leu Tyr Leu Ser Leu His Pro Gln Val Leu Glu Ile Phe Gly Phe 
        515                 520                 525             
Glu Gly Ala Asp Ala Glu Asp Val Ile Tyr Val Asn Trp Leu Asn Met 
    530                 535                 540                 
Val Arg Ala Gly Leu Leu Ala Leu Glu Phe Tyr Thr Pro Glu Ala Phe 
545                 550                 555                 560 
Asn Trp Arg Asp Val Leu Met Gln Ala Arg Phe Val Ile Leu Arg Val 
                565                 570                 575     
Leu Leu Glu Ala Gly Glu Gly Leu Val Thr Ile Thr Pro Thr Thr Gly 
            580                 585                 590         
Ser Asp Gly Arg Pro Asp Ala Arg Val Arg Leu Asp Arg Ser Lys Ile 
        595                 600                 605             
Arg Ser Val Gly Lys Pro Ala Leu Glu Arg Phe Leu Arg Arg Leu Gln 
    610                 615                 620                 
Val Leu Lys Ser Thr Gly Asp Val Ala Gly Gly Arg Ala Leu Tyr Glu 
625                 630                 635                 640 
Gly Tyr Ala Thr Val Thr Asp Ala Pro Pro Glu Ser Phe Leu Thr Leu 
                645                 650                 655     
Arg Asp Thr Val Leu Leu Arg Lys Glu Ser Arg Lys Leu Ile Val Gln 
            660                 665                 670         
Pro Asn Thr Arg Leu Glu Gly Ser Asp Val Gln Leu Leu Glu Tyr Glu 
        675                 680                 685             
Ala Ser Ala Ala Gly Leu Ile Arg Ser Phe Ser Glu Arg Phe Pro Glu 
    690                 695                 700                 
Asp Gly Pro Glu Leu Glu Glu Ile Leu Thr Gln Leu Ala Thr Ala Asp 
705                 710                 715                 720 
Ala Arg Phe Trp 
                

<210> 44
<211> 261
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 2FV5; mutant; M86

<400> 44
Ala Asp Pro Asp Pro Met Lys Asn Thr Cys Lys Leu Leu Val Val Ala 
1               5                   10                  15      
Asp His Arg Phe Tyr Arg Tyr Met Gly Arg Gly Glu Glu Ser Thr Thr 
            20                  25                  30          
Thr Asn Tyr Leu Ile Glu Leu Ile Asp Arg Val Asp Asp Ile Tyr Arg 
        35                  40                  45              
Asn Thr Ala Trp Asp Asn Ala Gly Phe Lys Gly Tyr Gly Ile Gln Ile 
    50                  55                  60                  
Glu Gln Ile Arg Ile Leu Lys Ser Pro Gln Glu Val Lys Pro Gly Glu 
65                  70                  75                  80  
Lys His Tyr Asn Met Ala Lys Ser Tyr Pro Asn Glu Glu Lys Asp Ala 
                85                  90                  95      
Trp Asp Leu Lys Met Leu Leu Glu Gln Phe Ser Phe Asp Ile Ala Glu 
            100                 105                 110         
Glu Ala Ser Lys Val Cys Leu Ala His Leu Phe Thr Tyr Gln Asp Phe 
        115                 120                 125             
Asp Met Gly Arg Leu Leu Ile Val Tyr Gly Gly Ser Pro His Ala Asn 
    130                 135                 140                 
Ser His Gly Gly Val Cys Pro Lys Ala Tyr Tyr Ser Pro Val Gly Lys 
145                 150                 155                 160 
Lys Asn Ile Tyr Leu Asn Ser Gly Leu Thr Ser Thr Lys Asn Tyr Gly 
                165                 170                 175     
Lys Thr Ile Leu Thr Lys Glu Ala Asp Leu Val Thr Thr His Ala Leu 
            180                 185                 190         
Gly His Asn Phe Gly Ala Glu His Asp Pro Asp Gly Leu Ala Glu Cys 
        195                 200                 205             
Ala Pro Asn Glu Asp Gln Gly Gly Lys Tyr Val Met Tyr Pro Ile Ala 
    210                 215                 220                 
Val Ser Gly Asp His Glu Asn Asn Lys Met Phe Ser Gln Cys Ser Lys 
225                 230                 235                 240 
Gln Ser Ile Tyr Lys Thr Ile Glu Ser Lys Ala Gln Glu Cys Phe Gln 
                245                 250                 255     
Glu Arg Ser Asn Ala 
            260     

<210> 45
<211> 249
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 5K7J; mutant; M93

<400> 45
Gly Ser Pro Arg Tyr Leu Asp Gly Trp Leu Lys Asp Val Val Gln Leu 
1               5                   10                  15      
Ser Leu Arg Arg Pro Ser Phe Arg Ala Ser Arg Gln Arg Pro Ile Ile 
            20                  25                  30          
Ser Leu Asn Glu Arg Ile Leu Glu Phe Asn Lys Arg Asn Ile Thr Ala 
        35                  40                  45              
Ile Ile Ala Glu Tyr Lys Arg Lys Ser Val Ser Gly Leu Asp Val Glu 
    50                  55                  60                  
Arg Asp Pro Ile Glu Tyr Ser Lys Phe Met Glu Arg Tyr Ala Val Gly 
65                  70                  75                  80  
Leu Ser Ile Leu Thr Glu Glu Lys Tyr Phe Asn Gly Ser Tyr Glu Thr 
                85                  90                  95      
Leu Arg Lys Ile Ala Ser Ser Val Ser Ile Pro Ile Leu Met Ala Asp 
            100                 105                 110         
Phe Ile Val Lys Glu Ser Gln Ile Asp Asp Ala Tyr Asn Leu Gly Ala 
        115                 120                 125             
Asp Thr Val Leu Leu Leu Val Met Ile Leu Thr Glu Arg Glu Leu Glu 
    130                 135                 140                 
Ser Leu Leu Glu Tyr Ala Arg Ser Tyr Gly Met Glu Pro Ile Ile Met 
145                 150                 155                 160 
Val Leu Asp Glu Asn Met Leu Asp Ile Ala Leu Arg Ile Gly Ala Arg 
                165                 170                 175     
Phe Ile Val Leu His Ser Lys Ser His Glu Thr Leu Glu Ile Asn Lys 
            180                 185                 190         
Glu Asn Gln Arg Lys Leu Ile Ser Met Ile Pro Ser Asn Val Val Lys 
        195                 200                 205             
Val Met Val His Gly Ile Ser Glu Arg Asn Glu Ile Glu Glu Leu Arg 
    210                 215                 220                 
Lys Leu Gly Val Asn Ala Phe Ile Ile Gly Ser Ser Leu Met Arg Asn 
225                 230                 235                 240 
Pro Glu Lys Ile Lys Glu Phe Ile Leu 
                245                 

<210> 46
<211> 201
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 1IAG; mutant; M93

<400> 46
Asn Leu Pro Gln Arg Tyr Ile Glu Leu Val Val Val Ala Asp Arg Arg 
1               5                   10                  15      
Val Phe Met Lys Tyr Asn Ser Asp Leu Asn Ile Ile Arg Thr Arg Val 
            20                  25                  30          
His Glu Ile Val Asn Ile Ile Asn Lys Phe Tyr Arg Ser Leu Asn Ile 
        35                  40                  45              
Arg Val Ser Leu Thr Asp Leu Glu Ile Trp Ser Gly Gln Asp Phe Ile 
    50                  55                  60                  
Thr Ile Gln Ser Ser Ser Ser Asn Thr Leu Asn Ser Phe Gly Glu Trp 
65                  70                  75                  80  
Arg Glu Arg Val Leu Leu Ile Trp Lys Arg His Asp Asn Ala Gln Leu 
                85                  90                  95      
Leu Thr Ala Ile Asn Phe Glu Gly Lys Ile Ile Met Leu Ala Tyr Thr 
            100                 105                 110         
Ser Ser Met Cys Asn Pro Arg Ser Ser Val Gly Ile Val Lys Asp His 
        115                 120                 125             
Ser Pro Ile Asn Leu Leu Val Ala Val Thr Met Ala His Leu Leu Gly 
    130                 135                 140                 
His Asn Leu Gly Met Glu His Asp Gly Lys Asp Cys Leu Arg Gly Ala 
145                 150                 155                 160 
Ser Leu Cys Ile Met Leu Pro Asp Leu Thr Pro Gly Arg Ser Tyr Glu 
                165                 170                 175     
Phe Ser Asp Asp Ser Met Gly Tyr Tyr Gln Lys Phe Leu Asn Gln Tyr 
            180                 185                 190         
Lys Pro Gln Cys Ile Leu Asn Lys Pro 
        195                 200     

<210> 47
<211> 866
<212> PRT
<213> Artificial Sequence


<220> 
<223> PDB ID: 4Q4E; mutant; M93

<400> 47
Pro Gln Ala Lys Tyr Arg His Asp Tyr Arg Ala Pro Asp Tyr Gln Ile 
1               5                   10                  15      
Thr Asp Ile Asp Leu Thr Phe Asp Leu Asp Ala Gln Lys Thr Val Val 
            20                  25                  30          
Thr Ala Val Ser Gln Ala Val Arg His Gly Ala Ser Asp Ala Pro Leu 
        35                  40                  45              
Arg Leu Asn Gly Glu Asp Leu Lys Leu Val Ser Val His Ile Asn Asp 
    50                  55                  60                  
Glu Pro Trp Thr Ala Trp Lys Glu Glu Glu Gly Ala Leu Val Ile Ser 
65                  70                  75                  80  
Asn Leu Pro Glu Arg Phe Thr Leu Lys Ile Ile Asn Glu Ile Ser Pro 
                85                  90                  95      
Ala Ala Asn Thr Ala Leu Glu Gly Leu Tyr Gln Ser Gly Asp Ala Leu 
            100                 105                 110         
Cys Thr Gln Cys Leu Ala Glu Gly Phe Arg His Ile Thr Tyr Tyr Leu 
        115                 120                 125             
Asp Arg Pro Asp Val Leu Ala Arg Phe Thr Thr Lys Ile Ile Ala Asp 
    130                 135                 140                 
Lys Ile Lys Tyr Pro Phe Leu Leu Ser Asn Gly Asn Arg Val Ala Gln 
145                 150                 155                 160 
Gly Glu Leu Glu Asn Gly Arg His Trp Val Gln Trp Gln Asp Pro Phe 
                165                 170                 175     
Pro Lys Pro Cys Tyr Leu Phe Ala Leu Val Ala Gly Asp Phe Asp Val 
            180                 185                 190         
Leu Arg Asp Thr Phe Thr Thr Arg Ser Gly Arg Glu Val Ala Leu Glu 
        195                 200                 205             
Leu Tyr Val Asp Arg Gly Asn Leu Asp Arg Ala Pro Trp Ala Met Thr 
    210                 215                 220                 
Ser Leu Lys Asn Ser Met Lys Trp Asp Glu Glu Arg Phe Gly Leu Glu 
225                 230                 235                 240 
Tyr Asp Leu Asp Ile Tyr Met Ile Val Ala Val Asp Phe Phe Asn Met 
                245                 250                 255     
Ile Val Ile Pro Asn Lys Gly Leu Asn Ile Phe Asn Ser Lys Tyr Val 
            260                 265                 270         
Leu Ala Arg Thr Asp Thr Ala Thr Asp Glu Asp Tyr Leu Lys Ile Glu 
        275                 280                 285             
Gln Ile Ile Gly His Ala Tyr Phe His Asn Trp Thr Gly Asn Arg Val 
    290                 295                 300                 
Thr Cys Arg Asp Trp Phe Gln Leu Ser Leu Ile Glu Gly Leu Thr Leu 
305                 310                 315                 320 
Phe Arg Val Gln Glu Phe Ser Ser Asp Leu Gly Ser Arg Ala Val Asn 
                325                 330                 335     
Arg Ile Asn Asn Val Arg Ile Met Arg Gly Leu Gln Phe Ala Glu Asp 
            340                 345                 350         
Ala Ser Pro Met Ala His Pro Ile Arg Pro Asp Met Val Ile Glu Met 
        355                 360                 365             
Asn Asn Phe Phe Thr Leu Thr Val Met Trp Lys Gly Ala Glu Val Ile 
    370                 375                 380                 
Arg Met Ile His Thr Leu Leu Gly Glu Glu Asn Phe Gln Lys Gly Met 
385                 390                 395                 400 
Gln Leu Tyr Phe Glu Arg His Asp Gly Ser Ala Ala Thr Cys Asp Asp 
                405                 410                 415     
Phe Val Gln Ala Met Glu Asp Ala Ser Asn Val Asp Leu Ser His Phe 
            420                 425                 430         
Arg Arg Trp Tyr Ser Gln Ser Gly Thr Pro Ile Val Thr Val Lys Asp 
        435                 440                 445             
Asp Tyr Asn Pro Glu Thr Glu Gln Tyr Thr Leu Thr Ile Ser Gln Arg 
    450                 455                 460                 
Thr Pro Ala Thr Pro Asp Gln Ala Glu Lys Gln Pro Leu His Ile Pro 
465                 470                 475                 480 
Phe Ala Ile Glu Leu Tyr Asp Asn Glu Gly Lys Val Ile Pro Leu Gln 
                485                 490                 495     
Lys Gly Gly His Pro Val Asn Ser Val Leu Asn Val Thr Gln Ala Glu 
            500                 505                 510         
Gln Thr Phe Val Phe Asp Asn Val Tyr Phe Gln Pro Val Pro Ala Leu 
        515                 520                 525             
Leu Cys Glu Phe Ser Ala Pro Val Lys Leu Glu Tyr Lys Trp Ser Asp 
    530                 535                 540                 
Gln Gln Leu Thr Phe Leu Met Arg His Ala Arg Asn Asp Phe Ser Arg 
545                 550                 555                 560 
Trp Asp Ala Ala Gln Ser Leu Leu Ala Thr Tyr Ile Lys Leu Asn Val 
                565                 570                 575     
Ala Arg His Gln Gln Gly Gln Pro Leu Ser Leu Pro Val His Val Ala 
            580                 585                 590         
Asp Ala Phe Arg Ala Val Leu Leu Asp Glu Lys Ile Asp Pro Ala Leu 
        595                 600                 605             
Ala Ala Glu Ile Leu Thr Leu Pro Ser Val Asn Glu Met Ala Glu Leu 
    610                 615                 620                 
Phe Asp Ile Ile Asp Pro Ile Ala Ile Ala Glu Val Arg Glu Ala Leu 
625                 630                 635                 640 
Thr Arg Thr Leu Ala Thr Glu Leu Ala Asp Glu Leu Leu Ala Ile Tyr 
                645                 650                 655     
Asn Ala Asn Tyr Gln Ser Glu Tyr Arg Val Glu His Glu Asp Ile Ala 
            660                 665                 670         
Lys Arg Thr Leu Arg Asn Ala Cys Leu Arg Phe Leu Ala Phe Gly Glu 
        675                 680                 685             
Thr His Leu Ala Asp Val Leu Val Ser Lys Gln Phe His Glu Ala Asn 
    690                 695                 700                 
Asn Met Thr Asp Ala Leu Ala Ala Leu Ser Ala Ala Val Ala Ala Gln 
705                 710                 715                 720 
Leu Pro Cys Arg Asp Ala Leu Met Gln Glu Tyr Asp Asp Lys Trp His 
                725                 730                 735     
Gln Asn Gly Leu Val Met Asp Lys Trp Phe Ile Leu Gln Ala Thr Ser 
            740                 745                 750         
Pro Ala Ala Asn Val Leu Glu Thr Val Arg Gly Leu Leu Gln His Arg 
        755                 760                 765             
Ser Phe Thr Met Ser Asn Pro Asn Arg Ile Arg Ser Leu Ile Gly Ala 
    770                 775                 780                 
Phe Ala Gly Ser Asn Pro Ala Ala Phe His Ala Glu Asp Gly Ser Gly 
785                 790                 795                 800 
Tyr Leu Phe Leu Val Glu Met Leu Thr Asp Leu Asn Ser Arg Asn Pro 
                805                 810                 815     
Gln Val Ala Ser Arg Leu Ile Glu Pro Leu Ile Arg Leu Lys Arg Tyr 
            820                 825                 830         
Asp Ala Lys Arg Gln Glu Lys Met Arg Ala Ala Leu Glu Gln Leu Lys 
        835                 840                 845             
Gly Leu Glu Asn Leu Ser Gly Asp Leu Tyr Glu Lys Ile Thr Lys Ala 
    850                 855                 860                 
Leu Ala 
865     

<210> 48
<211> 256
<212> PRT
<213> Artificial Sequence


<220> 
<223> Carbonic anhydrase II mutant, M=M64

<400> 48
His Trp Gly Tyr Gly Lys His Asn Gly Pro Glu His Trp His Lys Asp 
1               5                   10                  15      
Phe Pro Ile Ala Lys Gly Glu Arg Gln Ser Pro Val Asp Ile Asp Thr 
            20                  25                  30          
His Thr Ala Lys Tyr Asp Pro Ser Leu Lys Pro Leu Ser Val Ser Tyr 
        35                  40                  45              
Asp Gln Ala Thr Ser Leu Arg Ile Leu Asn Asn Gly His Ala Phe Asn 
    50                  55                  60                  
Val Glu Phe Asp Asp Ser Gln Asp Lys Ala Val Leu Lys Gly Gly Pro 
65                  70                  75                  80  
Leu Asp Gly Thr Tyr Arg Leu Ile Gln Phe His Phe His Trp Gly Ser 
                85                  90                  95      
Leu Asp Gly Gln Gly Ser Glu His Thr Val Asp Lys Lys Lys Tyr Ala 
            100                 105                 110         
Ala Glu Leu His Leu Val His Trp Asn Thr Lys Tyr Gly Asp Phe Gly 
        115                 120                 125             
Lys Ala Val Gln His Pro Asp Gly Leu Ala Val Leu Gly Ile Phe Leu 
    130                 135                 140                 
Lys Val Gly Ser Ala Lys Pro Gly Leu Gln Lys Val Val Asp Val Leu 
145                 150                 155                 160 
Asp Ser Ile Lys Thr Lys Gly Lys Ser Ala Asp Phe Thr Asn Phe Asp 
                165                 170                 175     
Pro Arg Gly Leu Leu Pro Glu Ser Leu Asp Tyr Trp Thr Tyr Pro Gly 
            180                 185                 190         
Ser Arg Thr Thr Pro Pro Leu Leu Glu Ser Val Thr Trp Ile Val Leu 
        195                 200                 205             
Lys Glu Pro Ile Ser Val Ser Ser Glu Gln Val Leu Lys Phe Arg Lys 
    210                 215                 220                 
Leu Asn Phe Asn Gly Glu Gly Glu Pro Glu Glu Leu Met Val Asp Asn 
225                 230                 235                 240 
Trp Arg Pro Ala Gln Pro Leu Lys Asn Arg Gln Ile Lys Ala Ser Phe 
                245                 250                 255     



<210> 49
<211> 256
<212> PRT
<213> Artificial Sequence


<220> 
<223> Carbonic anhydrase II mutant, M=M64

<400> 49
His Trp Gly Tyr Gly Lys His Asn Gly Pro Glu His Trp His Lys Asp 
1               5                   10                  15      
Phe Pro Ile Ala Lys Gly Glu Arg Gln Ser Pro Val Asp Ile Asp Thr 
            20                  25                  30          
His Thr Ala Lys Tyr Asp Pro Ser Leu Lys Pro Leu Ser Val Ser Tyr 
        35                  40                  45              
Asp Gln Ala Thr Ser Leu Arg Ile Leu Asn Asn Gly His Ala Phe Asn 
    50                  55                  60                  
Val Glu Phe Asp Asp Ser Gln Asp Lys Ala Val Leu Lys Gly Gly Pro 
65                  70                  75                  80  
Leu Asp Gly Thr Tyr Arg Leu Ile Gln Phe His Phe His Trp Gly Ser 
                85                  90                  95      
Leu Asp Gly Gln Gly Ser Glu His Thr Val Asp Lys Lys Lys Tyr Ala 
            100                 105                 110         
Ala Glu Leu His Leu Val His Trp Asn Thr Lys Tyr Gly Asp Phe Asp 
        115                 120                 125             
Lys Ala Val Arg Gln Pro Asp Gly Leu Ala Val Leu Gly Ile Phe Leu 
    130                 135                 140                 
Lys Val Gly Ser Ala Lys Pro Gly Leu Gln Lys Val Val Asp Val Leu 
145                 150                 155                 160 
Asp Ser Ile Lys Thr Lys Gly Lys Ser Ala Asp Phe Thr Asn Phe Asp 
                165                 170                 175     
Pro Arg Gly Leu Leu Pro Glu Ser Leu Asp Tyr Trp Thr Tyr Pro Gly 
            180                 185                 190         
Ser Arg Thr Thr Pro Pro Leu Leu Glu Ser Val Thr Trp Ile Val Leu 
        195                 200                 205             
Lys Glu Pro Ile Ser Val Ser Ser Glu Gln Val Leu Lys Phe Arg Lys 
    210                 215                 220                 
Leu Asn Phe Asn Gly Glu Gly Glu Pro Glu Glu Leu Met Val Asp Asn 
225                 230                 235                 240 
Trp Arg Pro Ala Gln Pro Leu Lys Asn Arg Gln Ile Lys Ala Ser Phe 
                245                 250                 255     



<210> 50
<211> 256
<212> PRT
<213> Artificial Sequence


<220> 
<223> Carbonic anhydrase II mutant, M=M64

<400> 50
His Trp Gly Tyr Gly Lys His Asn Gly Pro Glu His Trp His Lys Asp 
1               5                   10                  15      
Phe Pro Ile Ala Lys Gly Glu Arg Gln Ser Pro Val Asp Ile Asp Thr 
            20                  25                  30          
His Thr Ala Lys Tyr Asp Pro Ser Leu Lys Pro Leu Ser Val Ser Tyr 
        35                  40                  45              
Asp Gln Ala Thr Ser Leu Arg Ile Leu Asn Asn Gly His Ala Phe Asn 
    50                  55                  60                  
Val Glu Phe Asp Asp Ser Gln Asp Lys Ala Val Leu Lys Gly Gly Pro 
65                  70                  75                  80  
Leu Asp Gly Thr Tyr Arg Leu Ile Gln Phe His Phe His Trp Gly Ser 
                85                  90                  95      
Leu Asp Gly Gln Gly Ser Glu His Thr Val Asp Lys Lys Lys Tyr Ala 
            100                 105                 110         
Ala Glu Leu His Leu Val His Trp Asn Thr Lys Tyr Gly Asp Phe Asp 
        115                 120                 125             
Lys Ala Val Gln Gln Pro Asp Gly Leu Ala Val Leu Gly Ile Phe Leu 
    130                 135                 140                 
Asn Val Gly Ser Ala Arg Pro Gly Leu Gln Lys Val Val Asp Val Leu 
145                 150                 155                 160 
Asp Ser Ile Lys Thr Lys Gly Lys Ser Ala Tyr Phe Thr Asn Phe Asp 
                165                 170                 175     
Pro Arg Gly Leu Leu Pro Glu Ser Leu Asp Tyr Trp Thr Tyr Pro Gly 
            180                 185                 190         
Ser Arg Thr Thr Pro Pro Leu Leu Glu Ser Val Thr Trp Ile Val Leu 
        195                 200                 205             
Lys Glu Pro Ile Ser Val Ser Ser Glu Gln Ile Leu Lys Phe Arg Lys 
    210                 215                 220                 
Leu Asn Phe Asn Arg Glu Gly Glu Pro Glu Glu Leu Met Val Asp Asn 
225                 230                 235                 240 
Trp Arg Pro Thr Gln Pro Leu Lys Asn Arg Gln Ile Lys Ala Ser Phe 
                245                 250                 255     



<210> 51
<211> 256
<212> PRT
<213> Artificial Sequence


<220> 
<223> Carbonic anhydrase II mutant, M=M64

<400> 51
His Trp Gly Tyr Gly Lys His Asn Gly Pro Glu His Trp His Lys Asp 
1               5                   10                  15      
Phe Pro Ile Ala Lys Gly Glu Arg Gln Ser Pro Val Asp Ile Asp Thr 
            20                  25                  30          
His Thr Ala Lys Tyr Asp Pro Ser Leu Lys Pro Leu Ser Val Ser Tyr 
        35                  40                  45              
Asp Gln Ala Thr Ser Leu Arg Ile Leu Asn Asn Gly His Ala Phe Glu 
    50                  55                  60                  
Val Glu Phe Asp Asp Ser Gln Asp Lys Ala Val Leu Lys Gly Gly Pro 
65                  70                  75                  80  
Leu Asp Gly Thr Tyr Arg Leu Val Gln Phe His Phe His Trp Gly Ser 
                85                  90                  95      
Leu Asp Gly Gln Gly Ser Glu His Thr Val Asp Lys Lys Lys Tyr Ala 
            100                 105                 110         
Ala Glu Leu His Leu Val His Trp Asn Thr Lys Tyr Gly Asp Tyr Gly 
        115                 120                 125             
Lys Ala Met Gln Gln Pro Asp Gly Leu Ala Val Leu Gly Ile Phe Leu 
    130                 135                 140                 
Lys Val Gly Ser Ala Lys Pro Gly Leu Gln Lys Val Val Asp Val Leu 
145                 150                 155                 160 
Asp Ser Ile Lys Thr Lys Gly Lys Ser Ala Asp Phe Thr Asn Phe Asp 
                165                 170                 175     
Pro Arg Gly Leu Leu Pro Glu Ser Leu Asp Tyr Trp Thr Tyr Pro Gly 
            180                 185                 190         
Ser Gly Thr Val Pro Pro Leu Leu Glu Ser Val Thr Trp Ile Val Leu 
        195                 200                 205             
Lys Glu Pro Ile Ser Val Ser Ser Glu Gln Val Leu Lys Phe Arg Lys 
    210                 215                 220                 
Leu Asn Phe Asn Gly Glu Gly Glu Pro Glu Glu Leu Met Val Asp Asn 
225                 230                 235                 240 
Trp Arg Pro Ala Gln Pro Leu Lys Asn Arg Gln Ile Lys Ala Ser Phe 
                245                 250                 255     



<210> 52
<211> 256
<212> PRT
<213> Artificial Sequence


<220> 
<223> Carbonic anhydrase II mutant, M=M64

<400> 52
His Trp Gly Tyr Gly Lys His Asn Gly Pro Glu His Trp His Lys Asp 
1               5                   10                  15      
Phe Pro Ile Ala Lys Gly Glu Arg Gln Ser Pro Val Asp Ile Asp Thr 
            20                  25                  30          
His Thr Ala Lys Tyr Asp Pro Ser Leu Lys Pro Leu Ser Val Ser Tyr 
        35                  40                  45              
Asp Gln Ala Thr Ser Leu Arg Ile Leu Asn Asp Gly His Ala Phe Gln 
    50                  55                  60                  
Val Glu Phe Asp Asp Ser Gln Asp Lys Ala Val Leu Lys Gly Gly Pro 
65                  70                  75                  80  
Leu Asp Gly Thr Tyr Arg Leu Val Gln Phe His Phe His Trp Gly Ser 
                85                  90                  95      
Leu Asp Gly Gln Gly Ser Glu His Thr Val Asp Lys Lys Lys Tyr Ala 
            100                 105                 110         
Ala Glu Leu His Leu Val His Trp Asn Thr Lys Tyr Gly Asp Tyr Gly 
        115                 120                 125             
Lys Ala Met Gln Gln Pro Asp Gly Leu Ala Val Leu Gly Ile Phe Leu 
    130                 135                 140                 
Lys Val Gly Ser Ala Lys Pro Gly Leu Gln Lys Val Val Asp Val Leu 
145                 150                 155                 160 
Asp Ser Ile Lys Thr Lys Gly Lys Ser Ala Asp Phe Thr Asn Phe Asp 
                165                 170                 175     
Pro Arg Gly Leu Leu Pro Glu Ser Leu Asp Tyr Trp Thr Tyr Pro Gly 
            180                 185                 190         
Ser Gly Thr Val Pro Pro Leu Leu Glu Ser Val Thr Trp Ile Val Leu 
        195                 200                 205             
Lys Glu Pro Ile Ser Val Ser Ser Glu Gln Val Leu Lys Phe Arg Lys 
    210                 215                 220                 
Leu Asn Phe Asn Gly Glu Gly Glu Pro Glu Glu Leu Met Val Asp Asn 
225                 230                 235                 240 
Trp Arg Pro Ala Gln Pro Leu Lys Asn Arg Gln Ile Lys Ala Ser Phe 
                245                 250                 255     



<210> 53
<211> 256
<212> PRT
<213> Artificial Sequence


<220> 
<223> Carbonic anhydrase II mutant, M=M64

<400> 53
His Trp Gly Tyr Gly Lys His Asn Gly Pro Glu His Trp His Lys Asp 
1               5                   10                  15      
Phe Pro Ile Ala Lys Gly Glu Arg Gln Ser Pro Val Asp Ile Asp Thr 
            20                  25                  30          
His Thr Ala Lys Tyr Asp Pro Ser Leu Lys Pro Leu Ser Val Ser Tyr 
        35                  40                  45              
Asp Gln Ala Thr Ser Leu Arg Ile Leu Asn Asn Gly His Ala Phe Asn 
    50                  55                  60                  
Val Glu Phe Asp Asp Ser Gln Asp Lys Ala Val Leu Lys Gly Gly Pro 
65                  70                  75                  80  
Leu Asp Gly Thr Tyr Arg Leu Ile Gln Phe His Phe His Trp Gly Ser 
                85                  90                  95      
Leu Asp Gly Gln Gly Ser Glu His Thr Val Asp Lys Lys Lys Tyr Ala 
            100                 105                 110         
Ala Glu Leu His Leu Val His Trp Asn Thr Lys Tyr Gly Asp Phe Gly 
        115                 120                 125             
Lys Ala Met Gln Gln Pro Asp Gly Leu Ala Val Leu Gly Ile Phe Leu 
    130                 135                 140                 
Lys Val Gly Ser Ala Lys Pro Gly Leu Gln Lys Val Val Asp Val Leu 
145                 150                 155                 160 
Asp Ser Ile Lys Thr Lys Gly Lys Ser Ala Asp Phe Thr Asn Phe Asp 
                165                 170                 175     
Pro Arg Gly Leu Leu Pro Glu Ser Leu Asp Tyr Trp Thr Tyr Pro Gly 
            180                 185                 190         
Ser Gly Thr Val Pro Pro Leu Leu Glu Ser Val Thr Trp Ile Val Leu 
        195                 200                 205             
Lys Glu Pro Ile Ser Val Ser Ser Glu Gln Val Leu Lys Phe Arg Lys 
    210                 215                 220                 
Leu Asn Phe Asn Gly Glu Gly Glu Pro Glu Glu Leu Met Val Asp Asn 
225                 230                 235                 240 
Trp Arg Pro Ala Gln Pro Leu Lys Asn Arg Gln Ile Lys Ala Ser Phe 
                245                 250                 255     



<210> 54
<211> 256
<212> PRT
<213> Artificial Sequence


<220> 
<223> Carbonic anhydrase II mutant, M=M64

<400> 54
His Trp Gly Tyr Gly Lys His Asn Gly Pro Glu His Trp His Lys Asp 
1               5                   10                  15      
Phe Pro Ile Ala Lys Gly Glu Arg Gln Ser Pro Val Asp Ile Asp Thr 
            20                  25                  30          
His Thr Ala Lys Tyr Asp Pro Ser Leu Lys Pro Leu Ser Val Ser Tyr 
        35                  40                  45              
Asp Gln Ala Thr Ser Leu Arg Ile Leu Asn Asp Gly His Ala Phe Glu 
    50                  55                  60                  
Val Glu Phe Asp Asp Ser Gln Asp Lys Ala Val Leu Lys Gly Gly Pro 
65                  70                  75                  80  
Leu Asp Gly Thr Tyr Arg Leu Ile Gln Phe His Phe His Trp Gly Ser 
                85                  90                  95      
Leu Asp Gly Gln Gly Ser Glu His Thr Val Asp Lys Lys Lys Tyr Ala 
            100                 105                 110         
Ala Glu Leu His Leu Val His Trp Asn Thr Lys Tyr Gly Asp Phe Gly 
        115                 120                 125             
Lys Ala Leu Gln Gln Pro Asp Gly Met Ala Ile Leu Gly Ile Phe Leu 
    130                 135                 140                 
Lys Val Gly Ser Ala Lys Pro Gly Leu Gln Lys Val Val Asp Val Leu 
145                 150                 155                 160 
Asp Ser Ile Lys Thr Lys Gly Lys Ser Ala Asp Phe Thr Asn Phe Asp 
                165                 170                 175     
Pro Arg Gly Leu Leu Pro Glu Ser Leu Asp Tyr Trp Thr Tyr Pro Gly 
            180                 185                 190         
Ser Gln Thr Ile Pro Pro Leu Leu Glu Ser Val Thr Trp Ile Val Leu 
        195                 200                 205             
Lys Glu Pro Ile Ser Val Ser Ser Glu Gln Val Leu Lys Phe Arg Lys 
    210                 215                 220                 
Leu Asn Phe Asn Gly Glu Gly Glu Pro Glu Glu Leu Met Val Asp Asn 
225                 230                 235                 240 
Trp Arg Pro Ala Gln Pro Leu Lys Asn Arg Gln Ile Lys Ala Ser Phe 
                245                 250                 255     



<210> 55
<211> 256
<212> PRT
<213> Artificial Sequence


<220> 
<223> Carbonic anhydrase II mutant, M=M64

<400> 55
His Trp Gly Tyr Gly Lys His Asn Gly Pro Glu His Trp His Lys Asp 
1               5                   10                  15      
Phe Pro Ile Ala Lys Gly Glu Arg Gln Ser Pro Val Asp Ile Asp Thr 
            20                  25                  30          
His Thr Ala Lys Tyr Asp Pro Ser Leu Lys Pro Leu Ser Val Ser Tyr 
        35                  40                  45              
Asp Gln Ala Thr Ser Leu Arg Ile Leu Asn Asn Gly His Ala Phe Leu 
    50                  55                  60                  
Val Glu Phe Asp Asp Ser Gln Asp Lys Ala Val Leu Lys Gly Gly Pro 
65                  70                  75                  80  
Leu Asp Gly Thr Tyr Arg Leu Lys Gln Phe His Phe His Trp Gly Ser 
                85                  90                  95      
Leu Asp Gly Gln Gly Ser Glu His Thr Val Asp Lys Lys Lys Tyr Ala 
            100                 105                 110         
Ala Glu Leu His Leu Val His Trp Asn Thr Lys Tyr Gly Asp Tyr Gly 
        115                 120                 125             
Lys Ala Leu Gln Gln Pro Asp Gly Ile Ala Val Leu Gly Ile Phe Leu 
    130                 135                 140                 
Lys Val Gly Ser Ala Lys Pro Gly Leu Gln Lys Val Val Asp Val Leu 
145                 150                 155                 160 
Asp Ser Ile Lys Thr Lys Gly Lys Ser Ala Asp Phe Thr Asn Phe Asp 
                165                 170                 175     
Pro Arg Gly Leu Leu Pro Glu Ser Leu Asp Tyr Trp Thr Tyr Pro Gly 
            180                 185                 190         
Ser Arg Thr Ile Pro Pro Leu Leu Glu Ser Val Thr Trp Ile Val Leu 
        195                 200                 205             
Lys Glu Pro Ile Ser Val Ser Ser Glu Gln Val Leu Lys Phe Arg Lys 
    210                 215                 220                 
Leu Asn Phe Asn Gly Glu Gly Glu Pro Glu Glu Leu Met Val Asp Asn 
225                 230                 235                 240 
Trp Arg Pro Ala Gln Pro Leu Lys Asn Arg Gln Ile Lys Ala Ser Phe 
                245                 250                 255     



<210> 56
<211> 256
<212> PRT
<213> Artificial Sequence


<220> 
<223> Carbonic anhydrase II mutant, M=M64

<400> 56
His Trp Gly Tyr Gly Lys His Asn Gly Pro Glu His Trp His Lys Asp 
1               5                   10                  15      
Phe Pro Ile Ala Lys Gly Glu Arg Gln Ser Pro Val Asp Ile Asp Thr 
            20                  25                  30          
His Thr Ala Lys Tyr Asp Pro Ser Leu Lys Pro Leu Ser Val Ser Tyr 
        35                  40                  45              
Asp Gln Ala Thr Ser Leu Arg Ile Leu Asn Asn Gly His Ala Phe Leu 
    50                  55                  60                  
Val Glu Phe Asp Asp Ser Gln Asp Lys Ala Val Leu Lys Gly Gly Pro 
65                  70                  75                  80  
Leu Asp Gly Thr Tyr Arg Leu Ile Gln Phe His Phe His Trp Gly Ser 
                85                  90                  95      
Leu Asp Gly Gln Gly Ser Glu His Thr Val Asp Lys Lys Lys Tyr Ala 
            100                 105                 110         
Ala Glu Leu His Leu Val His Trp Asn Thr Lys Tyr Gly Asp Tyr Gly 
        115                 120                 125             
Lys Ala Leu Gln Gln Pro Asp Gly Ile Ala Val Leu Gly Ile Phe Leu 
    130                 135                 140                 
Lys Val Gly Ser Ala Lys Pro Gly Leu Gln Lys Val Val Asp Val Leu 
145                 150                 155                 160 
Asp Ser Ile Lys Thr Lys Gly Lys Ser Ala Asp Phe Thr Asn Phe Asp 
                165                 170                 175     
Pro Arg Gly Leu Leu Pro Glu Ser Leu Asp Tyr Trp Thr Tyr Pro Gly 
            180                 185                 190         
Ser Arg Thr Ile Pro Pro Leu Leu Glu Ser Val Thr Trp Ile Val Leu 
        195                 200                 205             
Lys Glu Pro Ile Ser Val Ser Ser Glu Gln Val Leu Lys Phe Arg Lys 
    210                 215                 220                 
Leu Asn Phe Asn Gly Glu Gly Glu Pro Glu Glu Leu Met Val Asp Asn 
225                 230                 235                 240 
Trp Arg Pro Ala Gln Pro Leu Lys Asn Arg Gln Ile Lys Ala Ser Phe 
                245                 250                 255     



<210> 57
<211> 256
<212> PRT
<213> Artificial Sequence


<220> 
<223> Carbonic anhydrase II mutant, M=M64

<400> 57
His Trp Gly Tyr Gly Lys His Asn Gly Pro Glu His Trp His Lys Asp 
1               5                   10                  15      
Phe Pro Ile Ala Lys Gly Glu Arg Gln Ser Pro Val Asp Ile Asp Thr 
            20                  25                  30          
His Thr Ala Lys Tyr Asp Pro Ser Leu Lys Pro Leu Ser Val Ser Tyr 
        35                  40                  45              
Asp Gln Ala Thr Ser Leu Arg Ile Leu Asn Asp Gly His Ala Phe Arg 
    50                  55                  60                  
Val Glu Phe Asp Asp Ser Gln Asp Lys Ala Val Leu Lys Gly Gly Pro 
65                  70                  75                  80  
Leu Asp Gly Thr Tyr Arg Leu Lys Gln Phe His Phe His Trp Gly Ser 
                85                  90                  95      
Leu Asp Gly Gln Gly Ser Glu His Thr Val Asp Lys Lys Lys Tyr Ala 
            100                 105                 110         
Ala Glu Leu His Leu Val His Trp Asn Thr Lys Tyr Gly Asp Phe Gly 
        115                 120                 125             
Lys Ala Gly Gln Gln Pro Asp Gly Ile Ala Ile Leu Gly Ile Phe Leu 
    130                 135                 140                 
Lys Val Gly Ser Ala Lys Pro Gly Leu Gln Lys Val Val Asp Val Leu 
145                 150                 155                 160 
Asp Ser Ile Lys Thr Lys Gly Lys Ser Ala Asp Phe Thr Asn Phe Asp 
                165                 170                 175     
Pro Arg Gly Leu Leu Pro Glu Ser Leu Asp Tyr Trp Thr Tyr Pro Gly 
            180                 185                 190         
Ser Glu Thr Ile Pro Pro Leu Leu Glu Ser Val Thr Trp Ile Val Leu 
        195                 200                 205             
Lys Glu Pro Ile Ser Val Ser Ser Glu Gln Val Leu Lys Phe Arg Lys 
    210                 215                 220                 
Leu Asn Phe Asn Gly Glu Gly Glu Pro Glu Glu Leu Met Val Asp Asn 
225                 230                 235                 240 
Trp Arg Pro Ala Gln Pro Leu Lys Asn Arg Gln Ile Lys Ala Ser Phe 
                245                 250                 255     



<210> 58
<211> 257
<212> PRT
<213> Artificial Sequence


<220> 
<223> Carbonic anhydrase I, M=M64

<400> 58
Asp Trp Gly Tyr Asp Asp Lys Asn Gly Pro Glu Gln Trp Ser Lys Leu 
1               5                   10                  15      
Tyr Pro Ile Ala Asn Gly Asn Asn Gln Ser Pro Val Asp Ile Lys Thr 
            20                  25                  30          
Ser Glu Thr Lys His Asp Thr Ser Leu Lys Pro Ile Ser Val Ser Tyr 
        35                  40                  45              
Asn Pro Ala Thr Ala Lys Glu Ile Ile Asn Val Gly His Ser Phe His 
    50                  55                  60                  
Val Asn Phe Glu Asp Asn Asp Asn Arg Ser Val Leu Lys Gly Gly Pro 
65                  70                  75                  80  
Phe Ser Asp Ser Tyr Arg Leu Phe Gln Phe His Phe His Trp Gly Ser 
                85                  90                  95      
Thr Asn Glu His Gly Ser Glu His Thr Val Asp Gly Val Lys Tyr Ser 
            100                 105                 110         
Ala Glu Leu His Val Ala His Trp Asn Ser Ala Lys Tyr Ser Ser Leu 
        115                 120                 125             
Ala Glu Ala Ala Ser Lys Ala Asp Gly Leu Ala Val Ile Gly Val Leu 
    130                 135                 140                 
Met Lys Val Gly Glu Ala Asn Pro Lys Leu Gln Lys Val Leu Asp Ala 
145                 150                 155                 160 
Leu Gln Ala Ile Lys Thr Lys Gly Lys Arg Ala Pro Phe Thr Asn Phe 
                165                 170                 175     
Asp Pro Ser Thr Leu Leu Pro Ser Ser Leu Asp Phe Trp Thr Tyr Pro 
            180                 185                 190         
Gly Ser Leu Thr His Pro Pro Leu Tyr Glu Ser Val Thr Trp Ile Ile 
        195                 200                 205             
Cys Lys Glu Ser Ile Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg 
    210                 215                 220                 
Ser Leu Leu Ser Asn Val Glu Gly Asp Asn Ala Val Pro Met Gln His 
225                 230                 235                 240 
Asn Asn Arg Pro Thr Gln Pro Leu Lys Gly Arg Thr Val Arg Ala Ser 
                245                 250                 255     
Phe 
    

<210> 59
<211> 722
<212> PRT
<213> Artificial Sequence


<220> 
<223> truncated 4Q4E scaffold

<400> 59
Pro Gln Ala Lys Tyr Arg His Asp Tyr Arg Ala Pro Asp Tyr Gln Ile 
1               5                   10                  15      
Thr Asp Ile Asp Leu Thr Phe Asp Leu Asp Ala Gln Lys Thr Val Val 
            20                  25                  30          
Thr Ala Val Ser Gln Ala Val Arg His Gly Ala Ser Asp Ala Pro Leu 
        35                  40                  45              
Arg Leu Asn Gly Glu Asp Leu Lys Leu Val Ser Val His Ile Asn Asp 
    50                  55                  60                  
Glu Pro Trp Thr Ala Trp Lys Glu Glu Glu Gly Ala Leu Val Ile Ser 
65                  70                  75                  80  
Asn Leu Pro Glu Arg Phe Thr Leu Lys Ile Ile Asn Glu Ile Ser Pro 
                85                  90                  95      
Ala Ala Asn Thr Ala Leu Glu Gly Leu Tyr Gln Ser Gly Asp Ala Leu 
            100                 105                 110         
Cys Thr Gln Cys Glu Ala Glu Gly Phe Arg His Ile Thr Tyr Tyr Leu 
        115                 120                 125             
Asp Arg Pro Asp Val Leu Ala Arg Phe Thr Thr Lys Ile Ile Ala Asp 
    130                 135                 140                 
Lys Ile Lys Tyr Pro Phe Leu Leu Ser Asn Gly Asn Arg Val Ala Gln 
145                 150                 155                 160 
Gly Glu Leu Glu Asn Gly Arg His Trp Val Gln Trp Gln Asp Pro Phe 
                165                 170                 175     
Pro Lys Pro Cys Tyr Leu Phe Ala Leu Val Ala Gly Asp Phe Asp Val 
            180                 185                 190         
Leu Arg Asp Thr Phe Thr Thr Arg Ser Gly Arg Glu Val Ala Leu Glu 
        195                 200                 205             
Leu Tyr Val Asp Arg Gly Asn Leu Asp Arg Ala Pro Trp Ala Met Thr 
    210                 215                 220                 
Ser Leu Lys Asn Ser Met Lys Trp Asp Glu Glu Arg Phe Gly Leu Glu 
225                 230                 235                 240 
Tyr Asp Leu Asp Ile Tyr Met Ile Val Ala Val Asp Phe Phe Asn Met 
                245                 250                 255     
Gly Ala Met Glu Asn Lys Gly Leu Asn Ile Phe Asn Ser Lys Tyr Val 
            260                 265                 270         
Leu Ala Arg Thr Asp Thr Ala Thr Asp Lys Asp Tyr Leu Asp Ile Glu 
        275                 280                 285             
Arg Val Ile Gly His Glu Tyr Phe His Asn Trp Thr Gly Asn Arg Val 
    290                 295                 300                 
Thr Cys Arg Asp Trp Phe Gln Leu Ser Leu Lys Glu Gly Leu Thr Val 
305                 310                 315                 320 
Phe Arg Asp Gln Glu Phe Ser Ser Asp Leu Gly Ser Arg Ala Val Asn 
                325                 330                 335     
Arg Ile Asn Asn Val Arg Thr Met Arg Gly Leu Gln Phe Ala Glu Asp 
            340                 345                 350         
Ala Ser Pro Met Ala His Pro Ile Arg Pro Asp Met Val Ile Glu Met 
        355                 360                 365             
Asn Asn Phe Tyr Thr Leu Thr Val Tyr Glu Lys Gly Ala Glu Val Ile 
    370                 375                 380                 
Arg Met Ile His Thr Leu Leu Gly Glu Glu Asn Phe Gln Lys Gly Met 
385                 390                 395                 400 
Gln Leu Tyr Phe Glu Arg His Asp Gly Ser Ala Ala Thr Cys Asp Asp 
                405                 410                 415     
Phe Val Gln Ala Met Glu Asp Ala Ser Asn Val Asp Leu Ser His Phe 
            420                 425                 430         
Arg Arg Trp Tyr Ser Gln Ser Gly Thr Pro Ile Val Thr Val Lys Asp 
        435                 440                 445             
Asp Tyr Asn Pro Glu Thr Glu Gln Tyr Thr Leu Thr Ile Ser Gln Arg 
    450                 455                 460                 
Thr Pro Ala Thr Pro Asp Gln Ala Glu Lys Gln Pro Leu His Ile Pro 
465                 470                 475                 480 
Phe Ala Ile Glu Leu Tyr Asp Asn Glu Gly Lys Val Ile Pro Leu Gln 
                485                 490                 495     
Lys Gly Gly His Pro Val Asn Ser Val Leu Asn Val Thr Gln Ala Glu 
            500                 505                 510         
Gln Thr Phe Val Phe Asp Asn Val Tyr Phe Gln Pro Val Pro Ala Leu 
        515                 520                 525             
Leu Cys Glu Phe Ser Ala Pro Val Lys Leu Glu Tyr Lys Trp Ser Asp 
    530                 535                 540                 
Gln Gln Leu Thr Phe Leu Met Arg His Ala Arg Asn Asp Phe Ser Arg 
545                 550                 555                 560 
Trp Asp Ala Ala Gln Ser Leu Leu Ala Thr Tyr Ile Lys Leu Asn Val 
                565                 570                 575     
Ala Arg His Gln Gln Gly Gln Pro Leu Ser Leu Pro Val His Val Ala 
            580                 585                 590         
Asp Ala Phe Arg Ala Val Leu Leu Asp Glu Lys Ile Asp Pro Ala Leu 
        595                 600                 605             
Ala Ala Glu Ile Leu Thr Leu Pro Ser Val Asn Glu Met Ala Glu Leu 
    610                 615                 620                 
Phe Asp Ile Ile Asp Pro Ile Ala Ile Ala Glu Val Arg Glu Ala Leu 
625                 630                 635                 640 
Thr Arg Thr Leu Ala Thr Glu Leu Ala Asp Glu Leu Leu Ala Ile Tyr 
                645                 650                 655     
Asn Ala Asn Tyr Gln Ser Glu Tyr Arg Val Glu His Glu Asp Ile Ala 
            660                 665                 670         
Lys Arg Thr Leu Arg Asn Ala Cys Leu Arg Phe Leu Ala Phe Gly Glu 
        675                 680                 685             
Thr His Leu Ala Asp Val Leu Val Ser Lys Gln Phe His Glu Ala Asn 
    690                 695                 700                 
Asn Met Thr Asp Ala Leu Ala Ala Leu Ser Ala Ala Val Ala Ala Gln 
705                 710                 715                 720 
Leu Pro 
        

<210> 60
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> model peptide

<400> 60
Ala Phe Ala Ala Glu 
1               5   

<210> 61
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> model peptide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is any standard L-amino acid residue

<400> 61
Xaa Ala Ala Ala Glu 
1               5   

<210> 62
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> model peptide

<400> 62
Asp Ala Glu Ile Arg 
1               5   

<210> 63
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> model peptide

<400> 63
Glu Ala Glu Ile Arg 
1               5   

<210> 64
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> model peptide

<400> 64
Ala Ala Glu Ile Arg 
1               5   

<210> 65
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> model peptide

<400> 65
Phe Ala Glu Ile Arg 
1               5   

<210> 66
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> model peptide

<400> 66
Leu Ala Glu Ile Arg 
1               5   

