<110>    CJ CheilJedang Corporation

<120>    GlxR protein variant or threonine production method using the
         same

<130>    OPP20213678KR

<150>    KR 10-2021-000369
<151>    2021-01-11

<160>    55

<170>    koPatentIn 3.0

<210>    1
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    GlxR_amino acid


<400>    1
Met Glu Gly Val Gln Glu Ile Leu Ser Arg Ala Gly Ile Phe Gln Gly
  1               5                  10                  15 

Val Asp Pro Thr Ala Val Asn Asn Leu Ile Gln Asp Met Glu Thr Val
             20                  25                  30 

Arg Phe Pro Arg Gly Ala Thr Ile Phe Asp Glu Gly Glu Pro Gly Asp
         35                  40                  45 

Arg Leu Tyr Ile Ile Thr Ser Gly Lys Val Lys Leu Ala Arg His Ala
     50                  55                  60 

Pro Asp Gly Arg Glu Asn Leu Leu Thr Ile Met Gly Pro Ser Asp Met
 65                  70                  75                  80 

Phe Gly Glu Leu Ser Ile Phe Asp Pro Gly Pro Arg Thr Ser Ser Ala
                 85                  90                  95 

Val Cys Val Thr Glu Val His Ala Ala Thr Met Asn Ser Asp Met Leu
            100                 105                 110 

Arg Asn Trp Val Ala Asp His Pro Ala Ile Ala Glu Gln Leu Leu Arg
        115                 120                 125 

Val Leu Ala Arg Arg Leu Arg Arg Thr Asn Ala Ser Leu Ala Asp Leu
    130                 135                 140 

Ile Phe Thr Asp Val Pro Gly Arg Val Ala Lys Thr Leu Leu Gln Leu
145                 150                 155                 160 

Ala Asn Arg Phe Gly Thr Gln Glu Ala Gly Ala Leu Arg Val Asn His
                165                 170                 175 

Asp Leu Thr Gln Glu Glu Ile Ala Gln Leu Val Gly Ala Ser Arg Glu
            180                 185                 190 

Thr Val Asn Lys Ala Leu Ala Thr Phe Ala His Arg Gly Trp Ile Arg
        195                 200                 205 

Leu Glu Gly Lys Ser Val Leu Ile Val Asp Thr Glu His Leu Ala Arg
    210                 215                 220 

Arg Ala Arg
225        


<210>    2
<211>    684
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR_nucleic acid


<400>    2
gtggaaggtg tacaggagat cctgtcgcgc gccggaattt ttcaaggcgt tgacccaacg         60

gcagtcaata acctcatcca ggatatggag accgttcgct tcccacgcgg agcaaccatc        120

ttcgacgagg gcgagccagg tgaccgcctt tacatcatca cctccggcaa agtgaagctt        180

gcgcgccacg caccggacgg ccgcgaaaac ctgctgacca tcatgggtcc ttccgacatg        240

ttcggtgagc tctccatctt cgacccaggc ccacgcacct cctctgcagt gtgtgtcacc        300

gaagttcatg cagcaaccat gaactctgac atgctgcgca actgggtagc tgaccaccca        360

gctatcgctg agcagctcct gcgcgttctg gctcgtcgtc tgcgtcgcac caacgcttcc        420

ctggctgacc tcatcttcac cgacgtccca ggccgcgttg ctaagaccct tctgcagctg        480

gctaaccgct tcggcaccca agaagctggc gcgctgcgcg tgaaccacga cctcactcag        540

gaagaaatcg cacagctcgt cggtgcttcc cgtgaaactg tgaataaggc tcttgcaacg        600

ttcgcacacc gtggctggat tcgcctcgag ggcaagtccg tcctcattgt ggacaccgag        660

catttggcac gtcgcgctcg ataa                                               684


<210>    3
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    GlxR_E45A


<400>    3
Met Glu Gly Val Gln Glu Ile Leu Ser Arg Ala Gly Ile Phe Gln Gly
  1               5                  10                  15 

Val Asp Pro Thr Ala Val Asn Asn Leu Ile Gln Asp Met Glu Thr Val
             20                  25                  30 

Arg Phe Pro Arg Gly Ala Thr Ile Phe Asp Glu Gly Ala Pro Gly Asp
         35                  40                  45 

Arg Leu Tyr Ile Ile Thr Ser Gly Lys Val Lys Leu Ala Arg His Ala
     50                  55                  60 

Pro Asp Gly Arg Glu Asn Leu Leu Thr Ile Met Gly Pro Ser Asp Met
 65                  70                  75                  80 

Phe Gly Glu Leu Ser Ile Phe Asp Pro Gly Pro Arg Thr Ser Ser Ala
                 85                  90                  95 

Val Cys Val Thr Glu Val His Ala Ala Thr Met Asn Ser Asp Met Leu
            100                 105                 110 

Arg Asn Trp Val Ala Asp His Pro Ala Ile Ala Glu Gln Leu Leu Arg
        115                 120                 125 

Val Leu Ala Arg Arg Leu Arg Arg Thr Asn Ala Ser Leu Ala Asp Leu
    130                 135                 140 

Ile Phe Thr Asp Val Pro Gly Arg Val Ala Lys Thr Leu Leu Gln Leu
145                 150                 155                 160 

Ala Asn Arg Phe Gly Thr Gln Glu Ala Gly Ala Leu Arg Val Asn His
                165                 170                 175 

Asp Leu Thr Gln Glu Glu Ile Ala Gln Leu Val Gly Ala Ser Arg Glu
            180                 185                 190 

Thr Val Asn Lys Ala Leu Ala Thr Phe Ala His Arg Gly Trp Ile Arg
        195                 200                 205 

Leu Glu Gly Lys Ser Val Leu Ile Val Asp Thr Glu His Leu Ala Arg
    210                 215                 220 

Arg Ala Arg
225        


<210>    4
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    GlxR_E45S


<400>    4
Met Glu Gly Val Gln Glu Ile Leu Ser Arg Ala Gly Ile Phe Gln Gly
  1               5                  10                  15 

Val Asp Pro Thr Ala Val Asn Asn Leu Ile Gln Asp Met Glu Thr Val
             20                  25                  30 

Arg Phe Pro Arg Gly Ala Thr Ile Phe Asp Glu Gly Ser Pro Gly Asp
         35                  40                  45 

Arg Leu Tyr Ile Ile Thr Ser Gly Lys Val Lys Leu Ala Arg His Ala
     50                  55                  60 

Pro Asp Gly Arg Glu Asn Leu Leu Thr Ile Met Gly Pro Ser Asp Met
 65                  70                  75                  80 

Phe Gly Glu Leu Ser Ile Phe Asp Pro Gly Pro Arg Thr Ser Ser Ala
                 85                  90                  95 

Val Cys Val Thr Glu Val His Ala Ala Thr Met Asn Ser Asp Met Leu
            100                 105                 110 

Arg Asn Trp Val Ala Asp His Pro Ala Ile Ala Glu Gln Leu Leu Arg
        115                 120                 125 

Val Leu Ala Arg Arg Leu Arg Arg Thr Asn Ala Ser Leu Ala Asp Leu
    130                 135                 140 

Ile Phe Thr Asp Val Pro Gly Arg Val Ala Lys Thr Leu Leu Gln Leu
145                 150                 155                 160 

Ala Asn Arg Phe Gly Thr Gln Glu Ala Gly Ala Leu Arg Val Asn His
                165                 170                 175 

Asp Leu Thr Gln Glu Glu Ile Ala Gln Leu Val Gly Ala Ser Arg Glu
            180                 185                 190 

Thr Val Asn Lys Ala Leu Ala Thr Phe Ala His Arg Gly Trp Ile Arg
        195                 200                 205 

Leu Glu Gly Lys Ser Val Leu Ile Val Asp Thr Glu His Leu Ala Arg
    210                 215                 220 

Arg Ala Arg
225        


<210>    5
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    GlxR_E45F


<400>    5
Met Glu Gly Val Gln Glu Ile Leu Ser Arg Ala Gly Ile Phe Gln Gly
  1               5                  10                  15 

Val Asp Pro Thr Ala Val Asn Asn Leu Ile Gln Asp Met Glu Thr Val
             20                  25                  30 

Arg Phe Pro Arg Gly Ala Thr Ile Phe Asp Glu Gly Phe Pro Gly Asp
         35                  40                  45 

Arg Leu Tyr Ile Ile Thr Ser Gly Lys Val Lys Leu Ala Arg His Ala
     50                  55                  60 

Pro Asp Gly Arg Glu Asn Leu Leu Thr Ile Met Gly Pro Ser Asp Met
 65                  70                  75                  80 

Phe Gly Glu Leu Ser Ile Phe Asp Pro Gly Pro Arg Thr Ser Ser Ala
                 85                  90                  95 

Val Cys Val Thr Glu Val His Ala Ala Thr Met Asn Ser Asp Met Leu
            100                 105                 110 

Arg Asn Trp Val Ala Asp His Pro Ala Ile Ala Glu Gln Leu Leu Arg
        115                 120                 125 

Val Leu Ala Arg Arg Leu Arg Arg Thr Asn Ala Ser Leu Ala Asp Leu
    130                 135                 140 

Ile Phe Thr Asp Val Pro Gly Arg Val Ala Lys Thr Leu Leu Gln Leu
145                 150                 155                 160 

Ala Asn Arg Phe Gly Thr Gln Glu Ala Gly Ala Leu Arg Val Asn His
                165                 170                 175 

Asp Leu Thr Gln Glu Glu Ile Ala Gln Leu Val Gly Ala Ser Arg Glu
            180                 185                 190 

Thr Val Asn Lys Ala Leu Ala Thr Phe Ala His Arg Gly Trp Ile Arg
        195                 200                 205 

Leu Glu Gly Lys Ser Val Leu Ile Val Asp Thr Glu His Leu Ala Arg
    210                 215                 220 

Arg Ala Arg
225        


<210>    6
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    GlxR_E45K


<400>    6
Met Glu Gly Val Gln Glu Ile Leu Ser Arg Ala Gly Ile Phe Gln Gly
  1               5                  10                  15 

Val Asp Pro Thr Ala Val Asn Asn Leu Ile Gln Asp Met Glu Thr Val
             20                  25                  30 

Arg Phe Pro Arg Gly Ala Thr Ile Phe Asp Glu Gly Lys Pro Gly Asp
         35                  40                  45 

Arg Leu Tyr Ile Ile Thr Ser Gly Lys Val Lys Leu Ala Arg His Ala
     50                  55                  60 

Pro Asp Gly Arg Glu Asn Leu Leu Thr Ile Met Gly Pro Ser Asp Met
 65                  70                  75                  80 

Phe Gly Glu Leu Ser Ile Phe Asp Pro Gly Pro Arg Thr Ser Ser Ala
                 85                  90                  95 

Val Cys Val Thr Glu Val His Ala Ala Thr Met Asn Ser Asp Met Leu
            100                 105                 110 

Arg Asn Trp Val Ala Asp His Pro Ala Ile Ala Glu Gln Leu Leu Arg
        115                 120                 125 

Val Leu Ala Arg Arg Leu Arg Arg Thr Asn Ala Ser Leu Ala Asp Leu
    130                 135                 140 

Ile Phe Thr Asp Val Pro Gly Arg Val Ala Lys Thr Leu Leu Gln Leu
145                 150                 155                 160 

Ala Asn Arg Phe Gly Thr Gln Glu Ala Gly Ala Leu Arg Val Asn His
                165                 170                 175 

Asp Leu Thr Gln Glu Glu Ile Ala Gln Leu Val Gly Ala Ser Arg Glu
            180                 185                 190 

Thr Val Asn Lys Ala Leu Ala Thr Phe Ala His Arg Gly Trp Ile Arg
        195                 200                 205 

Leu Glu Gly Lys Ser Val Leu Ile Val Asp Thr Glu His Leu Ala Arg
    210                 215                 220 

Arg Ala Arg
225        


<210>    7
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    GlxR_D66A


<400>    7
Met Glu Gly Val Gln Glu Ile Leu Ser Arg Ala Gly Ile Phe Gln Gly
  1               5                  10                  15 

Val Asp Pro Thr Ala Val Asn Asn Leu Ile Gln Asp Met Glu Thr Val
             20                  25                  30 

Arg Phe Pro Arg Gly Ala Thr Ile Phe Asp Glu Gly Glu Pro Gly Asp
         35                  40                  45 

Arg Leu Tyr Ile Ile Thr Ser Gly Lys Val Lys Leu Ala Arg His Ala
     50                  55                  60 

Pro Ala Gly Arg Glu Asn Leu Leu Thr Ile Met Gly Pro Ser Asp Met
 65                  70                  75                  80 

Phe Gly Glu Leu Ser Ile Phe Asp Pro Gly Pro Arg Thr Ser Ser Ala
                 85                  90                  95 

Val Cys Val Thr Glu Val His Ala Ala Thr Met Asn Ser Asp Met Leu
            100                 105                 110 

Arg Asn Trp Val Ala Asp His Pro Ala Ile Ala Glu Gln Leu Leu Arg
        115                 120                 125 

Val Leu Ala Arg Arg Leu Arg Arg Thr Asn Ala Ser Leu Ala Asp Leu
    130                 135                 140 

Ile Phe Thr Asp Val Pro Gly Arg Val Ala Lys Thr Leu Leu Gln Leu
145                 150                 155                 160 

Ala Asn Arg Phe Gly Thr Gln Glu Ala Gly Ala Leu Arg Val Asn His
                165                 170                 175 

Asp Leu Thr Gln Glu Glu Ile Ala Gln Leu Val Gly Ala Ser Arg Glu
            180                 185                 190 

Thr Val Asn Lys Ala Leu Ala Thr Phe Ala His Arg Gly Trp Ile Arg
        195                 200                 205 

Leu Glu Gly Lys Ser Val Leu Ile Val Asp Thr Glu His Leu Ala Arg
    210                 215                 220 

Arg Ala Arg
225        


<210>    8
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    GlxR_D66F


<400>    8
Met Glu Gly Val Gln Glu Ile Leu Ser Arg Ala Gly Ile Phe Gln Gly
  1               5                  10                  15 

Val Asp Pro Thr Ala Val Asn Asn Leu Ile Gln Asp Met Glu Thr Val
             20                  25                  30 

Arg Phe Pro Arg Gly Ala Thr Ile Phe Asp Glu Gly Glu Pro Gly Asp
         35                  40                  45 

Arg Leu Tyr Ile Ile Thr Ser Gly Lys Val Lys Leu Ala Arg His Ala
     50                  55                  60 

Pro Phe Gly Arg Glu Asn Leu Leu Thr Ile Met Gly Pro Ser Asp Met
 65                  70                  75                  80 

Phe Gly Glu Leu Ser Ile Phe Asp Pro Gly Pro Arg Thr Ser Ser Ala
                 85                  90                  95 

Val Cys Val Thr Glu Val His Ala Ala Thr Met Asn Ser Asp Met Leu
            100                 105                 110 

Arg Asn Trp Val Ala Asp His Pro Ala Ile Ala Glu Gln Leu Leu Arg
        115                 120                 125 

Val Leu Ala Arg Arg Leu Arg Arg Thr Asn Ala Ser Leu Ala Asp Leu
    130                 135                 140 

Ile Phe Thr Asp Val Pro Gly Arg Val Ala Lys Thr Leu Leu Gln Leu
145                 150                 155                 160 

Ala Asn Arg Phe Gly Thr Gln Glu Ala Gly Ala Leu Arg Val Asn His
                165                 170                 175 

Asp Leu Thr Gln Glu Glu Ile Ala Gln Leu Val Gly Ala Ser Arg Glu
            180                 185                 190 

Thr Val Asn Lys Ala Leu Ala Thr Phe Ala His Arg Gly Trp Ile Arg
        195                 200                 205 

Leu Glu Gly Lys Ser Val Leu Ile Val Asp Thr Glu His Leu Ala Arg
    210                 215                 220 

Arg Ala Arg
225        


<210>    9
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    GlxR_D66K


<400>    9
Met Glu Gly Val Gln Glu Ile Leu Ser Arg Ala Gly Ile Phe Gln Gly
  1               5                  10                  15 

Val Asp Pro Thr Ala Val Asn Asn Leu Ile Gln Asp Met Glu Thr Val
             20                  25                  30 

Arg Phe Pro Arg Gly Ala Thr Ile Phe Asp Glu Gly Glu Pro Gly Asp
         35                  40                  45 

Arg Leu Tyr Ile Ile Thr Ser Gly Lys Val Lys Leu Ala Arg His Ala
     50                  55                  60 

Pro Lys Gly Arg Glu Asn Leu Leu Thr Ile Met Gly Pro Ser Asp Met
 65                  70                  75                  80 

Phe Gly Glu Leu Ser Ile Phe Asp Pro Gly Pro Arg Thr Ser Ser Ala
                 85                  90                  95 

Val Cys Val Thr Glu Val His Ala Ala Thr Met Asn Ser Asp Met Leu
            100                 105                 110 

Arg Asn Trp Val Ala Asp His Pro Ala Ile Ala Glu Gln Leu Leu Arg
        115                 120                 125 

Val Leu Ala Arg Arg Leu Arg Arg Thr Asn Ala Ser Leu Ala Asp Leu
    130                 135                 140 

Ile Phe Thr Asp Val Pro Gly Arg Val Ala Lys Thr Leu Leu Gln Leu
145                 150                 155                 160 

Ala Asn Arg Phe Gly Thr Gln Glu Ala Gly Ala Leu Arg Val Asn His
                165                 170                 175 

Asp Leu Thr Gln Glu Glu Ile Ala Gln Leu Val Gly Ala Ser Arg Glu
            180                 185                 190 

Thr Val Asn Lys Ala Leu Ala Thr Phe Ala His Arg Gly Trp Ile Arg
        195                 200                 205 

Leu Glu Gly Lys Ser Val Leu Ile Val Asp Thr Glu His Leu Ala Arg
    210                 215                 220 

Arg Ala Arg
225        


<210>    10
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    GlxR_T166D


<400>    10
Met Glu Gly Val Gln Glu Ile Leu Ser Arg Ala Gly Ile Phe Gln Gly
  1               5                  10                  15 

Val Asp Pro Thr Ala Val Asn Asn Leu Ile Gln Asp Met Glu Thr Val
             20                  25                  30 

Arg Phe Pro Arg Gly Ala Thr Ile Phe Asp Glu Gly Glu Pro Gly Asp
         35                  40                  45 

Arg Leu Tyr Ile Ile Thr Ser Gly Lys Val Lys Leu Ala Arg His Ala
     50                  55                  60 

Pro Asp Gly Arg Glu Asn Leu Leu Thr Ile Met Gly Pro Ser Asp Met
 65                  70                  75                  80 

Phe Gly Glu Leu Ser Ile Phe Asp Pro Gly Pro Arg Thr Ser Ser Ala
                 85                  90                  95 

Val Cys Val Thr Glu Val His Ala Ala Thr Met Asn Ser Asp Met Leu
            100                 105                 110 

Arg Asn Trp Val Ala Asp His Pro Ala Ile Ala Glu Gln Leu Leu Arg
        115                 120                 125 

Val Leu Ala Arg Arg Leu Arg Arg Thr Asn Ala Ser Leu Ala Asp Leu
    130                 135                 140 

Ile Phe Thr Asp Val Pro Gly Arg Val Ala Lys Thr Leu Leu Gln Leu
145                 150                 155                 160 

Ala Asn Arg Phe Gly Asp Gln Glu Ala Gly Ala Leu Arg Val Asn His
                165                 170                 175 

Asp Leu Thr Gln Glu Glu Ile Ala Gln Leu Val Gly Ala Ser Arg Glu
            180                 185                 190 

Thr Val Asn Lys Ala Leu Ala Thr Phe Ala His Arg Gly Trp Ile Arg
        195                 200                 205 

Leu Glu Gly Lys Ser Val Leu Ile Val Asp Thr Glu His Leu Ala Arg
    210                 215                 220 

Arg Ala Arg
225        


<210>    11
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    GlxR_T166F


<400>    11
Met Glu Gly Val Gln Glu Ile Leu Ser Arg Ala Gly Ile Phe Gln Gly
  1               5                  10                  15 

Val Asp Pro Thr Ala Val Asn Asn Leu Ile Gln Asp Met Glu Thr Val
             20                  25                  30 

Arg Phe Pro Arg Gly Ala Thr Ile Phe Asp Glu Gly Glu Pro Gly Asp
         35                  40                  45 

Arg Leu Tyr Ile Ile Thr Ser Gly Lys Val Lys Leu Ala Arg His Ala
     50                  55                  60 

Pro Asp Gly Arg Glu Asn Leu Leu Thr Ile Met Gly Pro Ser Asp Met
 65                  70                  75                  80 

Phe Gly Glu Leu Ser Ile Phe Asp Pro Gly Pro Arg Thr Ser Ser Ala
                 85                  90                  95 

Val Cys Val Thr Glu Val His Ala Ala Thr Met Asn Ser Asp Met Leu
            100                 105                 110 

Arg Asn Trp Val Ala Asp His Pro Ala Ile Ala Glu Gln Leu Leu Arg
        115                 120                 125 

Val Leu Ala Arg Arg Leu Arg Arg Thr Asn Ala Ser Leu Ala Asp Leu
    130                 135                 140 

Ile Phe Thr Asp Val Pro Gly Arg Val Ala Lys Thr Leu Leu Gln Leu
145                 150                 155                 160 

Ala Asn Arg Phe Gly Phe Gln Glu Ala Gly Ala Leu Arg Val Asn His
                165                 170                 175 

Asp Leu Thr Gln Glu Glu Ile Ala Gln Leu Val Gly Ala Ser Arg Glu
            180                 185                 190 

Thr Val Asn Lys Ala Leu Ala Thr Phe Ala His Arg Gly Trp Ile Arg
        195                 200                 205 

Leu Glu Gly Lys Ser Val Leu Ile Val Asp Thr Glu His Leu Ala Arg
    210                 215                 220 

Arg Ala Arg
225        


<210>    12
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    GlxR_T166K


<400>    12
Met Glu Gly Val Gln Glu Ile Leu Ser Arg Ala Gly Ile Phe Gln Gly
  1               5                  10                  15 

Val Asp Pro Thr Ala Val Asn Asn Leu Ile Gln Asp Met Glu Thr Val
             20                  25                  30 

Arg Phe Pro Arg Gly Ala Thr Ile Phe Asp Glu Gly Glu Pro Gly Asp
         35                  40                  45 

Arg Leu Tyr Ile Ile Thr Ser Gly Lys Val Lys Leu Ala Arg His Ala
     50                  55                  60 

Pro Asp Gly Arg Glu Asn Leu Leu Thr Ile Met Gly Pro Ser Asp Met
 65                  70                  75                  80 

Phe Gly Glu Leu Ser Ile Phe Asp Pro Gly Pro Arg Thr Ser Ser Ala
                 85                  90                  95 

Val Cys Val Thr Glu Val His Ala Ala Thr Met Asn Ser Asp Met Leu
            100                 105                 110 

Arg Asn Trp Val Ala Asp His Pro Ala Ile Ala Glu Gln Leu Leu Arg
        115                 120                 125 

Val Leu Ala Arg Arg Leu Arg Arg Thr Asn Ala Ser Leu Ala Asp Leu
    130                 135                 140 

Ile Phe Thr Asp Val Pro Gly Arg Val Ala Lys Thr Leu Leu Gln Leu
145                 150                 155                 160 

Ala Asn Arg Phe Gly Lys Gln Glu Ala Gly Ala Leu Arg Val Asn His
                165                 170                 175 

Asp Leu Thr Gln Glu Glu Ile Ala Gln Leu Val Gly Ala Ser Arg Glu
            180                 185                 190 

Thr Val Asn Lys Ala Leu Ala Thr Phe Ala His Arg Gly Trp Ile Arg
        195                 200                 205 

Leu Glu Gly Lys Ser Val Leu Ile Val Asp Thr Glu His Leu Ala Arg
    210                 215                 220 

Arg Ala Arg
225        


<210>    13
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    GlxR_E168A


<400>    13
Met Glu Gly Val Gln Glu Ile Leu Ser Arg Ala Gly Ile Phe Gln Gly
  1               5                  10                  15 

Val Asp Pro Thr Ala Val Asn Asn Leu Ile Gln Asp Met Glu Thr Val
             20                  25                  30 

Arg Phe Pro Arg Gly Ala Thr Ile Phe Asp Glu Gly Glu Pro Gly Asp
         35                  40                  45 

Arg Leu Tyr Ile Ile Thr Ser Gly Lys Val Lys Leu Ala Arg His Ala
     50                  55                  60 

Pro Asp Gly Arg Glu Asn Leu Leu Thr Ile Met Gly Pro Ser Asp Met
 65                  70                  75                  80 

Phe Gly Glu Leu Ser Ile Phe Asp Pro Gly Pro Arg Thr Ser Ser Ala
                 85                  90                  95 

Val Cys Val Thr Glu Val His Ala Ala Thr Met Asn Ser Asp Met Leu
            100                 105                 110 

Arg Asn Trp Val Ala Asp His Pro Ala Ile Ala Glu Gln Leu Leu Arg
        115                 120                 125 

Val Leu Ala Arg Arg Leu Arg Arg Thr Asn Ala Ser Leu Ala Asp Leu
    130                 135                 140 

Ile Phe Thr Asp Val Pro Gly Arg Val Ala Lys Thr Leu Leu Gln Leu
145                 150                 155                 160 

Ala Asn Arg Phe Gly Thr Gln Ala Ala Gly Ala Leu Arg Val Asn His
                165                 170                 175 

Asp Leu Thr Gln Glu Glu Ile Ala Gln Leu Val Gly Ala Ser Arg Glu
            180                 185                 190 

Thr Val Asn Lys Ala Leu Ala Thr Phe Ala His Arg Gly Trp Ile Arg
        195                 200                 205 

Leu Glu Gly Lys Ser Val Leu Ile Val Asp Thr Glu His Leu Ala Arg
    210                 215                 220 

Arg Ala Arg
225        


<210>    14
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    GlxR_E168F


<400>    14
Met Glu Gly Val Gln Glu Ile Leu Ser Arg Ala Gly Ile Phe Gln Gly
  1               5                  10                  15 

Val Asp Pro Thr Ala Val Asn Asn Leu Ile Gln Asp Met Glu Thr Val
             20                  25                  30 

Arg Phe Pro Arg Gly Ala Thr Ile Phe Asp Glu Gly Glu Pro Gly Asp
         35                  40                  45 

Arg Leu Tyr Ile Ile Thr Ser Gly Lys Val Lys Leu Ala Arg His Ala
     50                  55                  60 

Pro Asp Gly Arg Glu Asn Leu Leu Thr Ile Met Gly Pro Ser Asp Met
 65                  70                  75                  80 

Phe Gly Glu Leu Ser Ile Phe Asp Pro Gly Pro Arg Thr Ser Ser Ala
                 85                  90                  95 

Val Cys Val Thr Glu Val His Ala Ala Thr Met Asn Ser Asp Met Leu
            100                 105                 110 

Arg Asn Trp Val Ala Asp His Pro Ala Ile Ala Glu Gln Leu Leu Arg
        115                 120                 125 

Val Leu Ala Arg Arg Leu Arg Arg Thr Asn Ala Ser Leu Ala Asp Leu
    130                 135                 140 

Ile Phe Thr Asp Val Pro Gly Arg Val Ala Lys Thr Leu Leu Gln Leu
145                 150                 155                 160 

Ala Asn Arg Phe Gly Thr Gln Phe Ala Gly Ala Leu Arg Val Asn His
                165                 170                 175 

Asp Leu Thr Gln Glu Glu Ile Ala Gln Leu Val Gly Ala Ser Arg Glu
            180                 185                 190 

Thr Val Asn Lys Ala Leu Ala Thr Phe Ala His Arg Gly Trp Ile Arg
        195                 200                 205 

Leu Glu Gly Lys Ser Val Leu Ile Val Asp Thr Glu His Leu Ala Arg
    210                 215                 220 

Arg Ala Arg
225        


<210>    15
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    GlxR_E168K


<400>    15
Met Glu Gly Val Gln Glu Ile Leu Ser Arg Ala Gly Ile Phe Gln Gly
  1               5                  10                  15 

Val Asp Pro Thr Ala Val Asn Asn Leu Ile Gln Asp Met Glu Thr Val
             20                  25                  30 

Arg Phe Pro Arg Gly Ala Thr Ile Phe Asp Glu Gly Glu Pro Gly Asp
         35                  40                  45 

Arg Leu Tyr Ile Ile Thr Ser Gly Lys Val Lys Leu Ala Arg His Ala
     50                  55                  60 

Pro Asp Gly Arg Glu Asn Leu Leu Thr Ile Met Gly Pro Ser Asp Met
 65                  70                  75                  80 

Phe Gly Glu Leu Ser Ile Phe Asp Pro Gly Pro Arg Thr Ser Ser Ala
                 85                  90                  95 

Val Cys Val Thr Glu Val His Ala Ala Thr Met Asn Ser Asp Met Leu
            100                 105                 110 

Arg Asn Trp Val Ala Asp His Pro Ala Ile Ala Glu Gln Leu Leu Arg
        115                 120                 125 

Val Leu Ala Arg Arg Leu Arg Arg Thr Asn Ala Ser Leu Ala Asp Leu
    130                 135                 140 

Ile Phe Thr Asp Val Pro Gly Arg Val Ala Lys Thr Leu Leu Gln Leu
145                 150                 155                 160 

Ala Asn Arg Phe Gly Thr Gln Lys Ala Gly Ala Leu Arg Val Asn His
                165                 170                 175 

Asp Leu Thr Gln Glu Glu Ile Ala Gln Leu Val Gly Ala Ser Arg Glu
            180                 185                 190 

Thr Val Asn Lys Ala Leu Ala Thr Phe Ala His Arg Gly Trp Ile Arg
        195                 200                 205 

Leu Glu Gly Lys Ser Val Leu Ile Val Asp Thr Glu His Leu Ala Arg
    210                 215                 220 

Arg Ala Arg
225        


<210>    16
<211>    35
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR L1


<400>    16
gaattcgagc tcggtaccct gagaccccac gcgag                                    35


<210>    17
<211>    36
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR R2


<400>    17
gactctagag gatccccatc gacggtgtca ccccac                                   36


<210>    18
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(E45A) L2


<400>    18
gtaaaggcgg tcacctggcg cgccctcgtc gaagatgg                                 38


<210>    19
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(E45A) R1


<400>    19
ccatcttcga cgagggcgcg ccaggtgacc gcctttac                                 38


<210>    20
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pDZ_F (pDZ vector sequencing primer_forward)


<400>    20
tattacgcca gctggcgaaa g                                                   21


<210>    21
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pDZ_R (pDZ vector sequencing primer_reverse)


<400>    21
ttccggctcg tatgttgtgt g                                                   21


<210>    22
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(E45S) L2


<400>    22
gtaaaggcgg tcacctggag agccctcgtc gaagatgg                                 38


<210>    23
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(E45S) R1


<400>    23
ccatcttcga cgagggctct ccaggtgacc gcctttac                                 38


<210>    24
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(E45F) L2


<400>    24
gtaaaggcgg tcacctggaa agccctcgtc gaagatgg                                 38


<210>    25
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(E45F) R1


<400>    25
ccatcttcga cgagggcttt ccaggtgacc gcctttac                                 38


<210>    26
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(E45K) L2


<400>    26
gtaaaggcgg tcacctggct tgccctcgtc gaagatgg                                 38


<210>    27
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(E45K) R1


<400>    27
ccatcttcga cgagggcaag ccaggtgacc gcctttac                                 38


<210>    28
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(D66A) L2


<400>    28
ggttttcgcg gcctgccggt gcgtggcgcg caag                                     34


<210>    29
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(D66A) R1


<400>    29
gcgccacgca ccggcaggcc gcgaaaacct gctg                                     34


<210>    30
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(D66F) L2


<400>    30
cagcaggttt tcgcggccaa acggtgcgtg gcgc                                     34


<210>    31
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(D66F) R1


<400>    31
gcgccacgca ccgtttggcc gcgaaaacct gctg                                     34


<210>    32
<211>    35
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(D66K) L2


<400>    32
ggttttcgcg gcctttccgg tgcgtggcgc gcaag                                    35


<210>    33
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(D66K) R1


<400>    33
gcgccacgca ccgaaaggcc gcgaaaacct gctg                                     34


<210>    34
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(T166D) L2


<400>    34
gccagcttct tggtcgccga agcggttagc cagc                                     34


<210>    35
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(T166D) R1


<400>    35
aaccgcttcg gcgaccaaga agctggcgcg ctg                                      33


<210>    36
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(T166F) L2


<400>    36
gccagcttct tggaagccga agcggttagc cagc                                     34


<210>    37
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(T166F) R1


<400>    37
aaccgcttcg gcttccaaga agctggcgcg ctg                                      33


<210>    38
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(T166K) L2


<400>    38
gccagcttct tgtttgccga agcggttagc cagc                                     34


<210>    39
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(T166K) R1


<400>    39
aaccgcttcg gcaaacaaga agctggcgcg ctg                                      33


<210>    40
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(E168A) L2


<400>    40
agcgcgccag cagcttgggc gccgaagcgg ttag                                     34


<210>    41
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(E168A) R1


<400>    41
cttcggcgcc caagctgctg gcgcgctgcg cgtg                                     34


<210>    42
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(E168F) L2


<400>    42
agcgcgccag caaattgggc gccgaagcgg ttag                                     34


<210>    43
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(E168F) R1


<400>    43
cttcggcgcc caatttgctg gcgcgctgcg cgtg                                     34


<210>    44
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(E168K) L2


<400>    44
agcgcgccag ccttttgggc gccgaagcgg ttag                                     34


<210>    45
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    glxR(E168K) R1


<400>    45
cttcggcgcc caaaaggctg gcgcgctgcg cgtg                                     34


<210>    46
<211>    421
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    LysC_L377K


<400>    46
Met Ala Leu Val Val Gln Lys Tyr Gly Gly Ser Ser Leu Glu Ser Ala
  1               5                  10                  15 

Glu Arg Ile Arg Asn Val Ala Glu Arg Ile Val Ala Thr Lys Lys Ala
             20                  25                  30 

Gly Asn Asp Val Val Val Val Cys Ser Ala Met Gly Asp Thr Thr Asp
         35                  40                  45 

Glu Leu Leu Glu Leu Ala Ala Ala Val Asn Pro Val Pro Pro Ala Arg
     50                  55                  60 

Glu Met Asp Met Leu Leu Thr Ala Gly Glu Arg Ile Ser Asn Ala Leu
 65                  70                  75                  80 

Val Ala Met Ala Ile Glu Ser Leu Gly Ala Glu Ala Gln Ser Phe Thr
                 85                  90                  95 

Gly Ser Gln Ala Gly Val Leu Thr Thr Glu Arg His Gly Asn Ala Arg
            100                 105                 110 

Ile Val Asp Val Thr Pro Gly Arg Val Arg Glu Ala Leu Asp Glu Gly
        115                 120                 125 

Lys Ile Cys Ile Val Ala Gly Phe Gln Gly Val Asn Lys Glu Thr Arg
    130                 135                 140 

Asp Val Thr Thr Leu Gly Arg Gly Gly Ser Asp Thr Thr Ala Val Ala
145                 150                 155                 160 

Leu Ala Ala Ala Leu Asn Ala Asp Val Cys Glu Ile Tyr Ser Asp Val
                165                 170                 175 

Asp Gly Val Tyr Thr Ala Asp Pro Arg Ile Val Pro Asn Ala Gln Lys
            180                 185                 190 

Leu Glu Lys Leu Ser Phe Glu Glu Met Leu Glu Leu Ala Ala Val Gly
        195                 200                 205 

Ser Lys Ile Leu Val Leu Arg Ser Val Glu Tyr Ala Arg Ala Phe Asn
    210                 215                 220 

Val Pro Leu Arg Val Arg Ser Ser Tyr Ser Asn Asp Pro Gly Thr Leu
225                 230                 235                 240 

Ile Ala Gly Ser Met Glu Asp Ile Pro Val Glu Glu Ala Val Leu Thr
                245                 250                 255 

Gly Val Ala Thr Asp Lys Ser Glu Ala Lys Val Thr Val Leu Gly Ile
            260                 265                 270 

Ser Asp Lys Pro Gly Glu Ala Ala Lys Val Phe Arg Ala Leu Ala Asp
        275                 280                 285 

Ala Glu Ile Asn Ile Asp Met Val Leu Gln Asn Val Ser Ser Val Glu
    290                 295                 300 

Asp Gly Thr Thr Asp Ile Thr Phe Thr Cys Pro Arg Ser Asp Gly Arg
305                 310                 315                 320 

Arg Ala Met Glu Ile Leu Lys Lys Leu Gln Val Gln Gly Asn Trp Thr
                325                 330                 335 

Asn Val Leu Tyr Asp Asp Gln Val Gly Lys Val Ser Leu Val Gly Ala
            340                 345                 350 

Gly Met Lys Ser His Pro Gly Val Thr Ala Glu Phe Met Glu Ala Leu
        355                 360                 365 

Arg Asp Val Asn Val Asn Ile Glu Lys Ile Ser Thr Ser Glu Ile Arg
    370                 375                 380 

Ile Ser Val Leu Ile Arg Glu Asp Asp Leu Asp Ala Ala Ala Arg Ala
385                 390                 395                 400 

Leu His Glu Gln Phe Gln Leu Gly Gly Glu Asp Glu Ala Val Val Tyr
                405                 410                 415 

Ala Gly Thr Gly Arg
            420    


<210>    47
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    lysC L1


<400>    47
tcgagctcgg tacccgctgc gcagtgttga atac                                     34


<210>    48
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    lysC R2


<400>    48
ctctagagga tccccgttca cctcagagac gatt                                     34


<210>    49
<211>    30
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    lysC(L377K) L2


<400>    49
tggaaatctt ttcgatgttc acgttgacat                                          30


<210>    50
<211>    30
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    lysC(L377K) R1


<400>    50
atgtcaacgt gaacatcgaa aagatttcca                                          30


<210>    51
<211>    445
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Hom_R407H


<400>    51
Met Thr Ser Ala Ser Ala Pro Ser Phe Asn Pro Gly Lys Gly Pro Gly
  1               5                  10                  15 

Ser Ala Val Gly Ile Ala Leu Leu Gly Phe Gly Thr Val Gly Thr Glu
             20                  25                  30 

Val Met Arg Leu Met Thr Glu Tyr Gly Asp Glu Leu Ala His Arg Ile
         35                  40                  45 

Gly Gly Pro Leu Glu Val Arg Gly Ile Ala Val Ser Asp Ile Ser Lys
     50                  55                  60 

Pro Arg Glu Gly Val Ala Pro Glu Leu Leu Thr Glu Asp Ala Phe Ala
 65                  70                  75                  80 

Leu Ile Glu Arg Glu Asp Val Asp Ile Val Val Glu Val Ile Gly Gly
                 85                  90                  95 

Ile Glu Tyr Pro Arg Glu Val Val Leu Ala Ala Leu Lys Ala Gly Lys
            100                 105                 110 

Ser Val Val Thr Ala Asn Lys Ala Leu Val Ala Ala His Ser Ala Glu
        115                 120                 125 

Leu Ala Asp Ala Ala Glu Ala Ala Asn Val Asp Leu Tyr Phe Glu Ala
    130                 135                 140 

Ala Val Ala Gly Ala Ile Pro Val Val Gly Pro Leu Arg Arg Ser Leu
145                 150                 155                 160 

Ala Gly Asp Gln Ile Gln Ser Val Met Gly Ile Val Asn Gly Thr Thr
                165                 170                 175 

Asn Phe Ile Leu Asp Ala Met Asp Ser Thr Gly Ala Asp Tyr Ala Asp
            180                 185                 190 

Ser Leu Ala Glu Ala Thr Arg Leu Gly Tyr Ala Glu Ala Asp Pro Thr
        195                 200                 205 

Ala Asp Val Glu Gly His Asp Ala Ala Ser Lys Ala Ala Ile Leu Ala
    210                 215                 220 

Ser Ile Ala Phe His Thr Arg Val Thr Ala Asp Asp Val Tyr Cys Glu
225                 230                 235                 240 

Gly Ile Ser Asn Ile Ser Ala Ala Asp Ile Glu Ala Ala Gln Gln Ala
                245                 250                 255 

Gly His Thr Ile Lys Leu Leu Ala Ile Cys Glu Lys Phe Thr Asn Lys
            260                 265                 270 

Glu Gly Lys Ser Ala Ile Ser Ala Arg Val His Pro Thr Leu Leu Pro
        275                 280                 285 

Val Ser His Pro Leu Ala Ser Val Asn Lys Ser Phe Asn Ala Ile Phe
    290                 295                 300 

Val Glu Ala Glu Ala Ala Gly Arg Leu Met Phe Tyr Gly Asn Gly Ala
305                 310                 315                 320 

Gly Gly Ala Pro Thr Ala Ser Ala Val Leu Gly Asp Val Val Gly Ala
                325                 330                 335 

Ala Arg Asn Lys Val His Gly Gly Arg Ala Pro Gly Glu Ser Thr Tyr
            340                 345                 350 

Ala Asn Leu Pro Ile Ala Asp Phe Gly Glu Thr Thr Thr Arg Tyr His
        355                 360                 365 

Leu Asp Met Asp Val Glu Asp Arg Val Gly Val Leu Ala Glu Leu Ala
    370                 375                 380 

Ser Leu Phe Ser Glu Gln Gly Ile Ser Leu Arg Thr Ile Arg Gln Glu
385                 390                 395                 400 

Glu Arg Asp Asp Asp Ala His Leu Ile Val Val Thr His Ser Ala Leu
                405                 410                 415 

Glu Ser Asp Leu Ser Arg Thr Val Glu Leu Leu Lys Ala Lys Pro Val
            420                 425                 430 

Val Lys Ala Ile Asn Ser Val Ile Arg Leu Glu Arg Asp
        435                 440                 445


<210>    52
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    hom L1


<400>    52
atcctctaga gtcgacccaa ctgcagacgt cgaa                                     34


<210>    53
<211>    35
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    hom R2


<400>    53
atgcctgcag gtcgacatag acagatttgt ccacg                                    35


<210>    54
<211>    35
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    hom(R407H) L2


<400>    54
gtgaccacga tcagatgtgc atcatcatcg cgctc                                    35


<210>    55
<211>    35
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    hom(R407H) R1


<400>    55
gcgatgatga tgcacatctg atcgtggtca cccac                                    35
