                         SEQUENCE LISTING

<110>  MIAMI UNIVERSITY
       Jones, John Andrew
       Gibbons Jr., William Jonathan
 
<120>  SYSTEMS AND METHODS FOR PHARMACEUTICAL PRODUCTION OF PSILOCYBIN 
       AND INTERMEDIATES OR SIDE PRODUCTS

<130>  315691-00018

<150>  US 63/135,864
<151>  2021-01-11

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  1320
<212>  DNA
<213>  Psilocybe cubensis

<400>  1
atgcaggtga tacccgcgtg caactcggca gcaataagat cactatgtcc tactcccgag       60

tcttttagaa acatgggatg gctctctgtc agcgatgcgg tctacagcga gttcatagga      120

gagttggcta cccgcgcttc caatcgaaat tactccaacg agttcggcct catgcaacct      180

atccaggaat tcaaggcttt cattgaaagc gacccggtgg tgcaccaaga atttattgac      240

atgttcgagg gcattcagga ctctccaagg aattatcagg aactatgtaa tatgttcaac      300

gatatctttc gcaaagctcc cgtctacgga gaccttggcc ctcccgttta tatgattatg      360

gccaaattaa tgaacacccg agcgggcttc tctgcattca cgagacaaag gttgaacctt      420

cacttcaaaa aacttttcga tacctgggga ttgttcctgt cttcgaaaga ttctcgaaat      480

gttcttgtgg ccgaccagtt cgacgacaga cattgcggct ggttgaacga gcgggccttg      540

tctgctatgg ttaaacatta caatggacgc gcatttgatg aagtcttcct ctgcgataaa      600

aatgccccat actacggctt caactcttac gacgacttct ttaatcgcag atttcgaaac      660

cgagatatcg accgacctgt agtcggtgga gttaacaaca ccaccctcat ttctgctgct      720

tgcgaatcac tttcctacaa cgtctcttat gacgtccagt ctctcgacac tttagttttc      780

aaaggagaga cttattcgct taagcatttg ctgaataatg accctttcac cccacaattc      840

gagcatggga gtattctaca aggattcttg aacgtcaccg cttaccaccg atggcacgca      900

cccgtcaatg ggacaatcgt caaaatcatc aacgttccag gtacctactt tgcgcaagcc      960

ccgagcacga ttggcgaccc tatcccggat aacgattacg acccacctcc ttaccttaag     1020

tctcttgtct acttctctaa tattgccgca aggcaaatta tgtttattga agccgacaac     1080

aaggaaattg gcctcatttt ccttgtgttc atcggcatga ccgaaatctc gacatgtgaa     1140

gccacggtgt ccgaaggtca acacgtcaat cgtggcgatg acttgggaat gttccatttc     1200

ggtggttctt cgttcgcgct tggtctgagg aaggattgca gggcagagat cgttgaaaag     1260

ttcaccgaac ccggaacagt gatcagaatc aacgaagtcg tcgctgctct aaaggcttag     1320


<210>  2
<211>  1089
<212>  DNA
<213>  Psilocybe cubensis

<400>  2
atggcgttcg atctcaagac tgaagacggc ctcatcacat atctcactaa acatctttct       60

ttggacgtcg acacgagcgg agtgaagcgc cttagcggag gctttgtcaa tgtaacctgg      120

cgcattaagc tcaatgctcc ttatcaaggt catacgagca tcatcctgaa gcatgctcag      180

ccgcacatgt ctacggatga ggattttaag ataggtgtag aacgttcggt ttacgaatac      240

caggctatca agctcatgat ggccaatcgg gaggttctgg gaggcgtgga tggcatagtt      300

tctgtgccag aaggcctgaa ctacgactta gagaataatg cattgatcat gcaagatgtc      360

gggaagatga agaccctttt agattatgtc accgccaaac cgccacttgc gacggatata      420

gcccgccttg ttgggacaga aattgggggg ttcgttgcca gactccataa cataggccgc      480

gagaggcgag acgatcctga gttcaaattc ttctctggaa atattgtcgg aaggacgact      540

tcagaccagc tgtatcaaac catcataccc aacgcagcga aatatggcgt cgatgacccc      600

ttgctgccta ctgtggttaa ggaccttgtg gacgatgtca tgcacagcga agagaccctt      660

gtcatggcgg acctgtggag tggaaatatt cttctccagt tggaggaggg aaacccatcg      720

aagctgcaga agatatatat cctggattgg gaactttgca agtacggccc agcgtcgttg      780

gacctgggct atttcttggg tgactgctat ttgatatccc gctttcaaga cgagcaggtc      840

ggtacgacga tgcggcaagc ctacttgcaa agctatgcgc gtacgagcaa gcattcgatc      900

aactacgcca aagtcactgc aggtattgct gctcatattg tgatgtggac cgactttatg      960

cagtggggga gcgaggaaga aaggataaat tttgtgaaaa agggggtagc tgcctttcac     1020

gacgccaggg gcaacaacga caatggggaa attacgtcta ccttactgaa ggaatcatcc     1080

actgcgtaa                                                             1089


<210>  3
<211>  930
<212>  DNA
<213>  Psilocybe cubensis

<400>  3
atgcatatca gaaatcctta ccgtacacca attgactatc aagcactttc agaggccttc       60

cctcccctca agccatttgt gtctgtcaat gcagatggta ccagttctgt tgacctcact      120

atcccagaag cccagagggc gttcacggcc gctcttcttc atcgtgactt cgggctcacc      180

atgaccatac cagaagaccg tctgtgccca acagtcccca ataggttgaa ctacgttctg      240

tggattgaag atattttcaa ctacacgaac aaaaccctcg gcctgtcgga tgaccgtcct      300

attaaaggcg ttgatattgg tacaggagcc tccgcaattt atcctatgct tgcctgtgct      360

cggttcaagg catggtctat ggttggaaca gaggtcgaga ggaagtgcat tgacacggcc      420

cgcctcaatg tcgtcgcgaa caatctccaa gaccgtctct cgatattaga gacatccatt      480

gatggtccta ttctcgtccc cattttcgag gcgactgaag aatacgaata cgagtttact      540

atgtgtaacc ctccattcta cgacggtgct gccgatatgc agacttcgga tgctgccaaa      600

ggatttggat ttggcgtggg cgctccccat tctggaacag tcatcgaaat gtcgactgag      660

ggaggtgaat cggctttcgt cgctcagatg gtccgtgaga gcttgaagct tcgaacacga      720

tgcagatggt acacgagtaa cttgggaaag ctgaaatcct tgaaagaaat agtggggctg      780

ctgaaagaac ttgagataag caactatgcc attaacgaat acgttcaggg gtccacacgt      840

cgttatgccg ttgcgtggtc tttcactgat attcaactgc ctgaggagct ttctcgtccc      900

tctaaccccg agctcagctc tcttttctag                                       930


<210>  4
<211>  439
<212>  PRT
<213>  Psilocybe cubensis

<400>  4

Met Gln Val Ile Pro Ala Cys Asn Ser Ala Ala Ile Arg Ser Leu Cys 
1               5                   10                  15      


Pro Thr Pro Glu Ser Phe Arg Asn Met Gly Trp Leu Ser Val Ser Asp 
            20                  25                  30          


Ala Val Tyr Ser Glu Phe Ile Gly Glu Leu Ala Thr Arg Ala Ser Asn 
        35                  40                  45              


Arg Asn Tyr Ser Asn Glu Phe Gly Leu Met Gln Pro Ile Gln Glu Phe 
    50                  55                  60                  


Lys Ala Phe Ile Glu Ser Asp Pro Val Val His Gln Glu Phe Ile Asp 
65                  70                  75                  80  


Met Phe Glu Gly Ile Gln Asp Ser Pro Arg Asn Tyr Gln Glu Leu Cys 
                85                  90                  95      


Asn Met Phe Asn Asp Ile Phe Arg Lys Ala Pro Val Tyr Gly Asp Leu 
            100                 105                 110         


Gly Pro Pro Val Tyr Met Ile Met Ala Lys Leu Met Asn Thr Arg Ala 
        115                 120                 125             


Gly Phe Ser Ala Phe Thr Arg Gln Arg Leu Asn Leu His Phe Lys Lys 
    130                 135                 140                 


Leu Phe Asp Thr Trp Gly Leu Phe Leu Ser Ser Lys Asp Ser Arg Asn 
145                 150                 155                 160 


Val Leu Val Ala Asp Gln Phe Asp Asp Arg His Cys Gly Trp Leu Asn 
                165                 170                 175     


Glu Arg Ala Leu Ser Ala Met Val Lys His Tyr Asn Gly Arg Ala Phe 
            180                 185                 190         


Asp Glu Val Phe Leu Cys Asp Lys Asn Ala Pro Tyr Tyr Gly Phe Asn 
        195                 200                 205             


Ser Tyr Asp Asp Phe Phe Asn Arg Arg Phe Arg Asn Arg Asp Ile Asp 
    210                 215                 220                 


Arg Pro Val Val Gly Gly Val Asn Asn Thr Thr Leu Ile Ser Ala Ala 
225                 230                 235                 240 


Cys Glu Ser Leu Ser Tyr Asn Val Ser Tyr Asp Val Gln Ser Leu Asp 
                245                 250                 255     


Thr Leu Val Phe Lys Gly Glu Thr Tyr Ser Leu Lys His Leu Leu Asn 
            260                 265                 270         


Asn Asp Pro Phe Thr Pro Gln Phe Glu His Gly Ser Ile Leu Gln Gly 
        275                 280                 285             


Phe Leu Asn Val Thr Ala Tyr His Arg Trp His Ala Pro Val Asn Gly 
    290                 295                 300                 


Thr Ile Val Lys Ile Ile Asn Val Pro Gly Thr Tyr Phe Ala Gln Ala 
305                 310                 315                 320 


Pro Ser Thr Ile Gly Asp Pro Ile Pro Asp Asn Asp Tyr Asp Pro Pro 
                325                 330                 335     


Pro Tyr Leu Lys Ser Leu Val Tyr Phe Ser Asn Ile Ala Ala Arg Gln 
            340                 345                 350         


Ile Met Phe Ile Glu Ala Asp Asn Lys Glu Ile Gly Leu Ile Phe Leu 
        355                 360                 365             


Val Phe Ile Gly Met Thr Glu Ile Ser Thr Cys Glu Ala Thr Val Ser 
    370                 375                 380                 


Glu Gly Gln His Val Asn Arg Gly Asp Asp Leu Gly Met Phe His Phe 
385                 390                 395                 400 


Gly Gly Ser Ser Phe Ala Leu Gly Leu Arg Lys Asp Cys Arg Ala Glu 
                405                 410                 415     


Ile Val Glu Lys Phe Thr Glu Pro Gly Thr Val Ile Arg Ile Asn Glu 
            420                 425                 430         


Val Val Ala Ala Leu Lys Ala 
        435                 


<210>  5
<211>  362
<212>  PRT
<213>  Psilocybe cubensis

<400>  5

Met Ala Phe Asp Leu Lys Thr Glu Asp Gly Leu Ile Thr Tyr Leu Thr 
1               5                   10                  15      


Lys His Leu Ser Leu Asp Val Asp Thr Ser Gly Val Lys Arg Leu Ser 
            20                  25                  30          


Gly Gly Phe Val Asn Val Thr Trp Arg Ile Lys Leu Asn Ala Pro Tyr 
        35                  40                  45              


Gln Gly His Thr Ser Ile Ile Leu Lys His Ala Gln Pro His Met Ser 
    50                  55                  60                  


Thr Asp Glu Asp Phe Lys Ile Gly Val Glu Arg Ser Val Tyr Glu Tyr 
65                  70                  75                  80  


Gln Ala Ile Lys Leu Met Met Ala Asn Arg Glu Val Leu Gly Gly Val 
                85                  90                  95      


Asp Gly Ile Val Ser Val Pro Glu Gly Leu Asn Tyr Asp Leu Glu Asn 
            100                 105                 110         


Asn Ala Leu Ile Met Gln Asp Val Gly Lys Met Lys Thr Leu Leu Asp 
        115                 120                 125             


Tyr Val Thr Ala Lys Pro Pro Leu Ala Thr Asp Ile Ala Arg Leu Val 
    130                 135                 140                 


Gly Thr Glu Ile Gly Gly Phe Val Ala Arg Leu His Asn Ile Gly Arg 
145                 150                 155                 160 


Glu Arg Arg Asp Asp Pro Glu Phe Lys Phe Phe Ser Gly Asn Ile Val 
                165                 170                 175     


Gly Arg Thr Thr Ser Asp Gln Leu Tyr Gln Thr Ile Ile Pro Asn Ala 
            180                 185                 190         


Ala Lys Tyr Gly Val Asp Asp Pro Leu Leu Pro Thr Val Val Lys Asp 
        195                 200                 205             


Leu Val Asp Asp Val Met His Ser Glu Glu Thr Leu Val Met Ala Asp 
    210                 215                 220                 


Leu Trp Ser Gly Asn Ile Leu Leu Gln Leu Glu Glu Gly Asn Pro Ser 
225                 230                 235                 240 


Lys Leu Gln Lys Ile Tyr Ile Leu Asp Trp Glu Leu Cys Lys Tyr Gly 
                245                 250                 255     


Pro Ala Ser Leu Asp Leu Gly Tyr Phe Leu Gly Asp Cys Tyr Leu Ile 
            260                 265                 270         


Ser Arg Phe Gln Asp Glu Gln Val Gly Thr Thr Met Arg Gln Ala Tyr 
        275                 280                 285             


Leu Gln Ser Tyr Ala Arg Thr Ser Lys His Ser Ile Asn Tyr Ala Lys 
    290                 295                 300                 


Val Thr Ala Gly Ile Ala Ala His Ile Val Met Trp Thr Asp Phe Met 
305                 310                 315                 320 


Gln Trp Gly Ser Glu Glu Glu Arg Ile Asn Phe Val Lys Lys Gly Val 
                325                 330                 335     


Ala Ala Phe His Asp Ala Arg Gly Asn Asn Asp Asn Gly Glu Ile Thr 
            340                 345                 350         


Ser Thr Leu Leu Lys Glu Ser Ser Thr Ala 
        355                 360         


<210>  6
<211>  309
<212>  PRT
<213>  Psilocybe cubensis

<400>  6

Met His Ile Arg Asn Pro Tyr Arg Thr Pro Ile Asp Tyr Gln Ala Leu 
1               5                   10                  15      


Ser Glu Ala Phe Pro Pro Leu Lys Pro Phe Val Ser Val Asn Ala Asp 
            20                  25                  30          


Gly Thr Ser Ser Val Asp Leu Thr Ile Pro Glu Ala Gln Arg Ala Phe 
        35                  40                  45              


Thr Ala Ala Leu Leu His Arg Asp Phe Gly Leu Thr Met Thr Ile Pro 
    50                  55                  60                  


Glu Asp Arg Leu Cys Pro Thr Val Pro Asn Arg Leu Asn Tyr Val Leu 
65                  70                  75                  80  


Trp Ile Glu Asp Ile Phe Asn Tyr Thr Asn Lys Thr Leu Gly Leu Ser 
                85                  90                  95      


Asp Asp Arg Pro Ile Lys Gly Val Asp Ile Gly Thr Gly Ala Ser Ala 
            100                 105                 110         


Ile Tyr Pro Met Leu Ala Cys Ala Arg Phe Lys Ala Trp Ser Met Val 
        115                 120                 125             


Gly Thr Glu Val Glu Arg Lys Cys Ile Asp Thr Ala Arg Leu Asn Val 
    130                 135                 140                 


Val Ala Asn Asn Leu Gln Asp Arg Leu Ser Ile Leu Glu Thr Ser Ile 
145                 150                 155                 160 


Asp Gly Pro Ile Leu Val Pro Ile Phe Glu Ala Thr Glu Glu Tyr Glu 
                165                 170                 175     


Tyr Glu Phe Thr Met Cys Asn Pro Pro Phe Tyr Asp Gly Ala Ala Asp 
            180                 185                 190         


Met Gln Thr Ser Asp Ala Ala Lys Gly Phe Gly Phe Gly Val Gly Ala 
        195                 200                 205             


Pro His Ser Gly Thr Val Ile Glu Met Ser Thr Glu Gly Gly Glu Ser 
    210                 215                 220                 


Ala Phe Val Ala Gln Met Val Arg Glu Ser Leu Lys Leu Arg Thr Arg 
225                 230                 235                 240 


Cys Arg Trp Tyr Thr Ser Asn Leu Gly Lys Leu Lys Ser Leu Lys Glu 
                245                 250                 255     


Ile Val Gly Leu Leu Lys Glu Leu Glu Ile Ser Asn Tyr Ala Ile Asn 
            260                 265                 270         


Glu Tyr Val Gln Gly Ser Thr Arg Arg Tyr Ala Val Ala Trp Ser Phe 
        275                 280                 285             


Thr Asp Ile Gln Leu Pro Glu Glu Leu Ser Arg Pro Ser Asn Pro Glu 
    290                 295                 300                 


Leu Ser Ser Leu Phe 
305                 


<210>  7
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H10 mutant T7 promoter

<400>  7
taatacgact cactacggaa gaa                                               23


<210>  8
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G6 mutant T7 promoter

<400>  8
taatacgact cactatttcg gaa                                               23


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H9 mutant T7 promoter

<400>  9
taatacgact cactaatact gaa                                               23


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C4 mutant T7 promoter

<400>  10
taatacgact cactatcaag gaa                                               23


<210>  11
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  consensus T7 promoter

<400>  11
taatacgact cactataggg gaa                                               23


<210>  12
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lac promoter

<400>  12
tttacacttt atgcttccgg ctcgtatgtt g                                      31


<210>  13
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lac UV5 promoter

<400>  13
tttacacttt atgcttccgg ctcgtataat g                                      31


<210>  14
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tac promoter

<400>  14
ttgacaatta atcatcggct cgtataatg                                         29


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trc promoter

<400>  15
ttgacaatta atcatccggc tcgtataatg                                        30


<210>  16
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAP promoter

<400>  16
gcgtaatgct taggcacagg attgatttgt cgcaatgatt gacacgattc cgcttgacgc       60

tgcgtaaggt ttttgtaatt ttacaggcaa ccttttattc a                          101


<210>  17
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  xylA promoter

<400>  17
ttgaaataaa catttatttt gtatatgatg agataaagtt agtttattgg ataaacaaac       60

taactcaatt aagatagttg atggataaac tt                                     92


<210>  18
<211>  8848
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pPsilo16 vector

<400>  18
taatacgact cactacggaa gaattgtgag cggataacaa ttcccctcta gaaataattt       60

tgtttaactt taagaaggag atatacatat ggctagcatg actggtggac agcaaatggg      120

tcgcggatcc atgcaggtga tacccgcgtg caactcggca gcaataagat cactatgtcc      180

tactcccgag tcttttagaa acatgggatg gctctctgtc agcgatgcgg tctacagcga      240

gttcatagga gagttggcta cccgcgcttc caatcgaaat tactccaacg agttcggcct      300

catgcaacct atccaggaat tcaaggcttt cattgaaagc gacccggtgg tgcaccaaga      360

atttattgac atgttcgagg gcattcagga ctctccaagg aattatcagg aactatgtaa      420

tatgttcaac gatatctttc gcaaagctcc cgtctacgga gaccttggcc ctcccgttta      480

tatgattatg gccaaattaa tgaacacccg agcgggcttc tctgcattca cgagacaaag      540

gttgaacctt cacttcaaaa aacttttcga tacctgggga ttgttcctgt cttcgaaaga      600

ttctcgaaat gttcttgtgg ccgaccagtt cgacgacaga cattgcggct ggttgaacga      660

gcgggccttg tctgctatgg ttaaacatta caatggacgc gcatttgatg aagtcttcct      720

ctgcgataaa aatgccccat actacggctt caactcttac gacgacttct ttaatcgcag      780

atttcgaaac cgagatatcg accgacctgt agtcggtgga gttaacaaca ccaccctcat      840

ttctgctgct tgcgaatcac tttcctacaa cgtctcttat gacgtccagt ctctcgacac      900

tttagttttc aaaggagaga cttattcgct taagcatttg ctgaataatg accctttcac      960

cccacaattc gagcatggga gtattctaca aggattcttg aacgtcaccg cttaccaccg     1020

atggcacgca cccgtcaatg ggacaatcgt caaaatcatc aacgttccag gtacctactt     1080

tgcgcaagcc ccgagcacga ttggcgaccc tatcccggat aacgattacg acccacctcc     1140

ttaccttaag tctcttgtct acttctctaa tattgccgca aggcaaatta tgtttattga     1200

agccgacaac aaggaaattg gcctcatttt ccttgtgttc atcggcatga ccgaaatctc     1260

gacatgtgaa gccacggtgt ccgaaggtca acacgtcaat cgtggcgatg acttgggaat     1320

gttccatttc ggtggttctt cgttcgcgct tggtctgagg aaggattgca gggcagagat     1380

cgttgaaaag ttcaccgaac ccggaacagt gatcagaatc aacgaagtcg tcgctgctct     1440

aaaggcttag aagcttgcgg ccgcactcga gtctggtaaa gaaaccgctg ctgcgaaatt     1500

tgaacgccag cacatggact cgtctactag aaataatttt gtttaacttt aagaaggaga     1560

tatacatatg gctagcatga ctggtggaca gcaaatgggt cgcggatcca tggcgttcga     1620

tctcaagact gaagacggcc tcatcacata tctcactaaa catctttctt tggacgtcga     1680

cacgagcgga gtgaagcgcc ttagcggagg ctttgtcaat gtaacctggc gcattaagct     1740

caatgctcct tatcaaggtc atacgagcat catcctgaag catgctcagc cgcacatgtc     1800

tacggatgag gattttaaga taggtgtaga acgttcggtt tacgaatacc aggctatcaa     1860

gctcatgatg gccaatcggg aggttctggg aggcgtggat ggcatagttt ctgtgccaga     1920

aggcctgaac tacgacttag agaataatgc attgatcatg caagatgtcg ggaagatgaa     1980

gaccctttta gattatgtca ccgccaaacc gccacttgcg acggatatag cccgccttgt     2040

tgggacagaa attggggggt tcgttgccag actccataac ataggccgcg agaggcgaga     2100

cgatcctgag ttcaaattct tctctggaaa tattgtcgga aggacgactt cagaccagct     2160

gtatcaaacc atcataccca acgcagcgaa atatggcgtc gatgacccct tgctgcctac     2220

tgtggttaag gaccttgtgg acgatgtcat gcacagcgaa gagacccttg tcatggcgga     2280

cctgtggagt ggaaatattc ttctccagtt ggaggaggga aacccatcga agctgcagaa     2340

gatatatatc ctggattggg aactttgcaa gtacggccca gcgtcgttgg acctgggcta     2400

tttcttgggt gactgctatt tgatatcccg ctttcaagac gagcaggtcg gtacgacgat     2460

gcggcaagcc tacttgcaaa gctatgcgcg tacgagcaag cattcgatca actacgccaa     2520

agtcactgca ggtattgctg ctcatattgt gatgtggacc gactttatgc agtgggggag     2580

cgaggaagaa aggataaatt ttgtgaaaaa gggggtagct gcctttcacg acgccagggg     2640

caacaacgac aatggggaaa ttacgtctac cttactgaag gaatcatcca ctgcgtaaaa     2700

gcttgcggcc gcactcgagt ctggtaaaga aaccgctgct gcgaaatttg aacgccagca     2760

catggactcg tctactagaa ataattttgt ttaactttaa gaaggagata tacatatggc     2820

tagcatgact ggtggacagc aaatgggtcg cggatccatg catatcagaa atccttaccg     2880

tacaccaatt gactatcaag cactttcaga ggccttccct cccctcaagc catttgtgtc     2940

tgtcaatgca gatggtacca gttctgttga cctcactatc ccagaagccc agagggcgtt     3000

cacggccgct cttcttcatc gtgacttcgg gctcaccatg accataccag aagaccgtct     3060

gtgcccaaca gtccccaata ggttgaacta cgttctgtgg attgaagata ttttcaacta     3120

cacgaacaaa accctcggcc tgtcggatga ccgtcctatt aaaggcgttg atattggtac     3180

aggagcctcc gcaatttatc ctatgcttgc ctgtgctcgg ttcaaggcat ggtctatggt     3240

tggaacagag gtcgagagga agtgcattga cacggcccgc ctcaatgtcg tcgcgaacaa     3300

tctccaagac cgtctctcga tattagagac atccattgat ggtcctattc tcgtccccat     3360

tttcgaggcg actgaagaat acgaatacga gtttactatg tgtaaccctc cattctacga     3420

cggtgctgcc gatatgcaga cttcggatgc tgccaaagga tttggatttg gcgtgggcgc     3480

tccccattct ggaacagtca tcgaaatgtc gactgaggga ggtgaatcgg ctttcgtcgc     3540

tcagatggtc cgtgagagct tgaagcttcg aacacgatgc agatggtaca cgagtaactt     3600

gggaaagctg aaatccttga aagaaatagt ggggctgctg aaagaacttg agataagcaa     3660

ctatgccatt aacgaatacg ttcaggggtc cacacgtcgt tatgccgttg cgtggtcttt     3720

cactgatatt caactgcctg aggagctttc tcgtccctct aaccccgagc tcagctctct     3780

tttctagctc gagtctggta aagaaaccgc tgctgcgaaa tttgaacgcc agcacatgga     3840

ctcgtctact agtcgcagct taattaacct aaactgctgc caccgctgag caataactag     3900

cataacccct tggggcctct aaacgggtct tgaggggttt tttgctagcg aaaggaggag     3960

tcgactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg     4020

gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt     4080

tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc     4140

gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg     4200

attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga     4260

cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc     4320

ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa     4380

aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa     4440

tttctggcgg cacgatggca tgagattatc aaaaaggatc ttcacctaga tccttttaaa     4500

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta     4560

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt     4620

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag     4680

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca     4740

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc     4800

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt     4860

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag     4920

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt     4980

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat     5040

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt     5100

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc     5160

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat     5220

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag     5280

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt     5340

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg     5400

gaaatgttga atactcatac tcttcctttt tcaatcatga ttgaagcatt tatcagggtt     5460

attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggtcatg     5520

accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc     5580

aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa     5640

ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag     5700

gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta     5760

ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta     5820

ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag     5880

ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg     5940

gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg     6000

cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag     6060

cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc     6120

cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa     6180

aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg     6240

ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct     6300

gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa     6360

gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat     6420

ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct     6480

atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc     6540

ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag     6600

ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag     6660

ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc     6720

gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc     6780

ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt     6840

aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc     6900

ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag     6960

aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg     7020

tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg     7080

cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc     7140

agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta     7200

accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgct     7260

agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg     7320

tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc     7380

cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg     7440

gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca     7500

acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg     7560

tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc     7620

tgtcttcggt atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact     7680

cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg     7740

gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt     7800

cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag     7860

ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt     7920

gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa     7980

tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg     8040

cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga     8100

cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta     8160

ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa     8220

tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt     8280

tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt     8340

ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg     8400

tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca     8460

ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc     8520

attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag     8580

cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag     8640

atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg     8700

ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg     8760

ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagcctagg     8820

atcgagatcg atctcgatcc cgcgaaat                                        8848


<210>  19
<211>  7774
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pETM6-C4-psiDK vector

<400>  19
taatacgact cactatcaag gaattgtgag cggataacaa ttcccctcta gaaataattt       60

tgtttaactt taagaaggag atatacatat ggctagcatg actggtggac agcaaatggg      120

tcgcggatcc atgcaggtga tacccgcgtg caactcggca gcaataagat cactatgtcc      180

tactcccgag tcttttagaa acatgggatg gctctctgtc agcgatgcgg tctacagcga      240

gttcatagga gagttggcta cccgcgcttc caatcgaaat tactccaacg agttcggcct      300

catgcaacct atccaggaat tcaaggcttt cattgaaagc gacccggtgg tgcaccaaga      360

atttattgac atgttcgagg gcattcagga ctctccaagg aattatcagg aactatgtaa      420

tatgttcaac gatatctttc gcaaagctcc cgtctacgga gaccttggcc ctcccgttta      480

tatgattatg gccaaattaa tgaacacccg agcgggcttc tctgcattca cgagacaaag      540

gttgaacctt cacttcaaaa aacttttcga tacctgggga ttgttcctgt cttcgaaaga      600

ttctcgaaat gttcttgtgg ccgaccagtt cgacgacaga cattgcggct ggttgaacga      660

gcgggccttg tctgctatgg ttaaacatta caatggacgc gcatttgatg aagtcttcct      720

ctgcgataaa aatgccccat actacggctt caactcttac gacgacttct ttaatcgcag      780

atttcgaaac cgagatatcg accgacctgt agtcggtgga gttaacaaca ccaccctcat      840

ttctgctgct tgcgaatcac tttcctacaa cgtctcttat gacgtccagt ctctcgacac      900

tttagttttc aaaggagaga cttattcgct taagcatttg ctgaataatg accctttcac      960

cccacaattc gagcatggga gtattctaca aggattcttg aacgtcaccg cttaccaccg     1020

atggcacgca cccgtcaatg ggacaatcgt caaaatcatc aacgttccag gtacctactt     1080

tgcgcaagcc ccgagcacga ttggcgaccc tatcccggat aacgattacg acccacctcc     1140

ttaccttaag tctcttgtct acttctctaa tattgccgca aggcaaatta tgtttattga     1200

agccgacaac aaggaaattg gcctcatttt ccttgtgttc atcggcatga ccgaaatctc     1260

gacatgtgaa gccacggtgt ccgaaggtca acacgtcaat cgtggcgatg acttgggaat     1320

gttccatttc ggtggttctt cgttcgcgct tggtctgagg aaggattgca gggcagagat     1380

cgttgaaaag ttcaccgaac ccggaacagt gatcagaatc aacgaagtcg tcgctgctct     1440

aaaggcttag aagcttgcgg ccgcactcga gtctggtaaa gaaaccgctg ctgcgaaatt     1500

tgaacgccag cacatggact cgtctactag aaataatttt gtttaacttt aagaaggaga     1560

tatacatatg gctagcatga ctggtggaca gcaaatgggt cgcggatcca tggcgttcga     1620

tctcaagact gaagacggcc tcatcacata tctcactaaa catctttctt tggacgtcga     1680

cacgagcgga gtgaagcgcc ttagcggagg ctttgtcaat gtaacctggc gcattaagct     1740

caatgctcct tatcaaggtc atacgagcat catcctgaag catgctcagc cgcacatgtc     1800

tacggatgag gattttaaga taggtgtaga acgttcggtt tacgaatacc aggctatcaa     1860

gctcatgatg gccaatcggg aggttctggg aggcgtggat ggcatagttt ctgtgccaga     1920

aggcctgaac tacgacttag agaataatgc attgatcatg caagatgtcg ggaagatgaa     1980

gaccctttta gattatgtca ccgccaaacc gccacttgcg acggatatag cccgccttgt     2040

tgggacagaa attggggggt tcgttgccag actccataac ataggccgcg agaggcgaga     2100

cgatcctgag ttcaaattct tctctggaaa tattgtcgga aggacgactt cagaccagct     2160

gtatcaaacc atcataccca acgcagcgaa atatggcgtc gatgacccct tgctgcctac     2220

tgtggttaag gaccttgtgg acgatgtcat gcacagcgaa gagacccttg tcatggcgga     2280

cctgtggagt ggaaatattc ttctccagtt ggaggaggga aacccatcga agctgcagaa     2340

gatatatatc ctggattggg aactttgcaa gtacggccca gcgtcgttgg acctgggcta     2400

tttcttgggt gactgctatt tgatatcccg ctttcaagac gagcaggtcg gtacgacgat     2460

gcggcaagcc tacttgcaaa gctatgcgcg tacgagcaag cattcgatca actacgccaa     2520

agtcactgca ggtattgctg ctcatattgt gatgtggacc gactttatgc agtgggggag     2580

cgaggaagaa aggataaatt ttgtgaaaaa gggggtagct gcctttcacg acgccagggg     2640

caacaacgac aatggggaaa ttacgtctac cttactgaag gaatcatcca ctgcgtaaaa     2700

gcttgcggcc gcactcgagt ctggtaaaga aaccgctgct gcgaaatttg aacgccagca     2760

catggactcg tctactagtc gcagcttaat taacctaaac tgctgccacc gctgagcaat     2820

aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctagcgaaag     2880

gaggagtcga ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg     2940

cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg     3000

ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc     3060

taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa     3120

aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc     3180

ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac     3240

tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt     3300

ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt     3360

ttacaatttc tggcggcacg atggcatgag attatcaaaa aggatcttca cctagatcct     3420

tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga     3480

cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc     3540

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg     3600

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat     3660

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat     3720

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg     3780

caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc     3840

attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa     3900

agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc     3960

actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt     4020

ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag     4080

ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt     4140

gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag     4200

atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac     4260

cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc     4320

gacacggaaa tgttgaatac tcatactctt cctttttcaa tcatgattga agcatttatc     4380

agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag     4440

gtcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa     4500

aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca     4560

aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt     4620

ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg     4680

tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc     4740

ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga     4800

cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc     4860

agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc     4920

gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca     4980

ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg     5040

tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta     5100

tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct     5160

cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag     5220

tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa     5280

gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc     5340

atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac     5400

tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga     5460

cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc     5520

cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg     5580

gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc     5640

cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt     5700

aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat     5760

gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga     5820

acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga     5880

ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc     5940

acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga     6000

cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca     6060

ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt     6120

ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat     6180

catgctagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg     6240

catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc     6300

actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg     6360

cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga     6420

cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca     6480

cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac     6540

atgagctgtc ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc     6600

cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg     6660

cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac     6720

tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc     6780

agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt     6840

gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga     6900

aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag     6960

tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc     7020

cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc     7080

gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg     7140

cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg     7200

actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg     7260

ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg     7320

aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca     7380

cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt     7440

tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg     7500

aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc     7560

aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa     7620

caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata     7680

taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag     7740

cctaggatcg agatcgatct cgatcccgcg aaat                                 7774


<210>  20
<211>  7774
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pNOR

<400>  20
taatacgact cactatcaag gaattgtgag cggataacaa ttcccctcta gaaataattt       60

tgtttaactt taagaaggag atatacatat ggctagcatg actggtggac agcaaatggg      120

tcgcggatcc atgcaggtga tacccgcgtg caactcggca gcaataagat cactatgtcc      180

tactcccgag tcttttagaa acatgggatg gctctctgtc agcgatgcgg tctacagcga      240

gttcatagga gagttggcta cccgcgcttc caatcgaaat tactccaacg agttcggcct      300

catgcaacct atccaggaat tcaaggcttt cattgaaagc gacccggtgg tgcaccaaga      360

atttattgac atgttcgagg gcattcagga ctctccaagg aattatcagg aactatgtaa      420

tatgttcaac gatatctttc gcaaagctcc cgtctacgga gaccttggcc ctcccgttta      480

tatgattatg gccaaattaa tgaacacccg agcgggcttc tctgcattca cgagacaaag      540

gttgaacctt cacttcaaaa aacttttcga tacctgggga ttgttcctgt cttcgaaaga      600

ttctcgaaat gttcttgtgg ccgaccagtt cgacgacaga cattgcggct ggttgaacga      660

gcgggccttg tctgctatgg ttaaacatta caatggacgc gcatttgatg aagtcttcct      720

ctgcgataaa aatgccccat actacggctt caactcttac gacgacttct ttaatcgcag      780

atttcgaaac cgagatatcg accgacctgt agtcggtgga gttaacaaca ccaccctcat      840

ttctgctgct tgcgaatcac tttcctacaa cgtctcttat gacgtccagt ctctcgacac      900

tttagttttc aaaggagaga cttattcgct taagcatttg ctgaataatg accctttcac      960

cccacaattc gagcatggga gtattctaca aggattcttg aacgtcaccg cttaccaccg     1020

atggcacgca cccgtcaatg ggacaatcgt caaaatcatc aacgttccag gtacctactt     1080

tgcgcaagcc ccgagcacga ttggcgaccc tatcccggat aacgattacg acccacctcc     1140

ttaccttaag tctcttgtct acttctctaa tattgccgca aggcaaatta tgtttattga     1200

agccgacaac aaggaaattg gcctcatttt ccttgtgttc atcggcatga ccgaaatctc     1260

gacatgtgaa gccacggtgt ccgaaggtca acacgtcaat cgtggcgatg acttgggaat     1320

gttccatttc ggtggttctt cgttcgcgct tggtctgagg aaggattgca gggcagagat     1380

cgttgaaaag ttcaccgaac ccggaacagt gatcagaatc aacgaagtcg tcgctgctct     1440

aaaggcttag aagcttgcgg ccgcactcga gtctggtaaa gaaaccgctg ctgcgaaatt     1500

tgaacgccag cacatggact cgtctactag aaataatttt gtttaacttt aagaaggaga     1560

tatacatatg gctagcatga ctggtggaca gcaaatgggt cgcggatcca tggcgttcga     1620

tctcaagact gaagacggcc tcatcacata tctcactaaa catctttctt tggacgtcga     1680

cacgagcgga gtgaagcgcc ttagcggagg ctttgtcaat gtaacctggc gcattaagct     1740

caatgctcct tatcaaggtc atacgagcat catcctgaag catgctcagc cgcacatgtc     1800

tacggatgag gattttaaga taggtgtaga acgttcggtt tacgaatacc aggctatcaa     1860

gctcatgatg gccaatcggg aggttctggg aggcgtggat ggcatagttt ctgtgccaga     1920

aggcctgaac tacgacttag agaataatgc attgatcatg caagatgtcg ggaagatgaa     1980

gaccctttta gattatgtca ccgccaaacc gccacttgcg acggatatag cccgccttgt     2040

tgggacagaa attggggggt tcgttgccag actccataac ataggccgcg agaggcgaga     2100

cgatcctgag ttcaaattct tctctggaaa tattgtcgga aggacgactt cagaccagct     2160

gtatcaaacc atcataccca acgcagcgaa atatggcgtc gatgacccct tgctgcctac     2220

tgtggttaag gaccttgtgg acgatgtcat gcacagcgaa gagacccttg tcatggcgga     2280

cctgtggagt ggaaatattc ttctccagtt ggaggaggga aacccatcga agctgcagaa     2340

gatatatatc ctggattggg aactttgcaa gtacggccca gcgtcgttgg acctgggcta     2400

tttcttgggt gactgctatt tgatatcccg ctttcaagac gagcaggtcg gtacgacgat     2460

gcggcaagcc tacttgcaaa gctatgcgcg tacgagcaag cattcgatca actacgccaa     2520

agtcactgca ggtattgctg ctcatattgt gatgtggacc gactttatgc agtgggggag     2580

cgaggaagaa aggataaatt ttgtgaaaaa gggggtagct gcctttcacg acgccagggg     2640

caacaacgac aatggggaaa ttacgtctac cttactgaag gaatcatcca ctgcgtaaaa     2700

gcttgcggcc gcactcgagt ctggtaaaga aaccgctgct gcgaaatttg aacgccagca     2760

catggactcg tctactagtc gcagcttaat taacctaaac tgctgccacc gctgagcaat     2820

aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctagcgaaag     2880

gaggagtcga ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg     2940

cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg     3000

ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc     3060

taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa     3120

aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc     3180

ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac     3240

tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt     3300

ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt     3360

ttacaatttc tggcggcacg atggcatgag attatcaaaa aggatcttca cctagatcct     3420

tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga     3480

cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc     3540

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg     3600

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat     3660

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat     3720

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg     3780

caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc     3840

attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa     3900

agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc     3960

actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt     4020

ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag     4080

ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt     4140

gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag     4200

atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac     4260

cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc     4320

gacacggaaa tgttgaatac tcatactctt cctttttcaa tcatgattga agcatttatc     4380

agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag     4440

gtcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa     4500

aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca     4560

aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt     4620

ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg     4680

tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc     4740

ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga     4800

cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc     4860

agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc     4920

gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca     4980

ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg     5040

tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta     5100

tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct     5160

cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag     5220

tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa     5280

gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc     5340

atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac     5400

tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga     5460

cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc     5520

cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg     5580

gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc     5640

cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt     5700

aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat     5760

gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga     5820

acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga     5880

ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc     5940

acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga     6000

cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca     6060

ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt     6120

ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat     6180

catgctagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg     6240

catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc     6300

actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg     6360

cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga     6420

cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca     6480

cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac     6540

atgagctgtc ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc     6600

cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg     6660

cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac     6720

tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc     6780

agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt     6840

gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga     6900

aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag     6960

tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc     7020

cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc     7080

gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg     7140

cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg     7200

actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg     7260

ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg     7320

aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca     7380

cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt     7440

tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg     7500

aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc     7560

aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa     7620

caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata     7680

taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag     7740

cctaggatcg agatcgatct cgatcccgcg aaat                                 7774


