                         SEQUENCE LISTING

<110>  Regents of the University of Minnesota
       Webber, Beau
       Moriarity, Branden
       Lahr, Walker
       Slipek, Nicholas
 
<120>  ENHANCING EFFICIENCY OF TARGETED GENE KNOCKIN BY BASE EDITORS

<130>  920171.00453

<150>  63/140,032
<151>  2021-01-21

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockin at AAVS1 locus

<400>  1
gtcaccaatc ctgtccctag                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockin at AAVS1 locus

<400>  2
gtcgccggtc ctgtccctag                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockin at AAVS1 locus

<400>  3
gttattaatc ctgtccctag                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockin at AAVS1 locus

<400>  4
cttcctagtc tcctgatatt                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockin at AAVS1 locus

<400>  5
cttcctggtc tcctgatatt                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockin at AAVS1 locus

<400>  6
ctttttagtc tcctgatatt                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of PDCD1

<400>  7
cacctaccta agaaccatcc                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of PDCD1

<400>  8
ggagtctgag agatggagag                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of PDCD1

<400>  9
ttctctctgg aagggcacaa                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of PDCD1

<400>  10
gacgttacct cgtgcggccc                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of PDCD1

<400>  11
cctgcagaga aacacacttg                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of PDCD1

<400>  12
ggggttccag ggcctgtctg                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of PDCD1

<400>  13
cagttccaaa ccctggtggt                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of PDCD1

<400>  14
ggacccagac tagcagcacc                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of TRAC

<400>  15
cttacctggg ctggggaaga                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of TRAC

<400>  16
ttcgtatctg taaaaccaag                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of TRAC

<400>  17
tttcaaaacc tgtcagtgat                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of TRAC

<400>  18
ttcaaaacct gtcagtgatt                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of B2M

<400>  19
actcacgctg gatagcctcc                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of B2M

<400>  20
tcgatctatg aaaaagacag                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of B2M

<400>  21
cttaccccac ttaactatct                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of CD3E

<400>  22
actcacctga taagaggcag                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of PDCD1

<400>  23
cacctaccta agaaccatcc                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of B2M

<400>  24
cttaccccac ttaactatct                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockout of CISH

<400>  25
ctcaccagat tcccgaaggt                                                   20


<210>  26
<211>  3004
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - DNA donor template encoding GFP

<400>  26
tgctttctct gacctgcatt ctctcccctg ggcctgtgcc gctttctgtc tgcagcttgt       60

ggcctgggtc acctctacgg ctggcccaga tccttccctg ccgcctcctt caggttccgt      120

cttcctccac tccctcttcc ccttgctctc tgctgtgttg ctgcccaagg atgctctttc      180

cggagcactt ccttctcggc gctgcaccac gtgatgtcct ctgagcggat cctccccgtg      240

tctgggtcct ctccgggcat ctctcctccc tcacccaacc ccatgccgtc ttcactcgct      300

gggttccctt ttccttctcc ttctggggcc tgtgccatct ctcgtttctt aggatggcct      360

tctccgacgg atgtctccct tgcgtcccgc ctccccttct tgtaggcctg catcatcacc      420

gtttttctgg acaaccccaa agtaccccgt ctccctggct ttagccacct ctccatcctc      480

ttgctttctt tgcctggaca ccccgttctc ctgtggattc gggtcacctc tcactccttt      540

catttgggca gctcccctac cccccttacc tctctagtct gtgctagctc ttccagcccc      600

ctgtcatggc atcttccagg ggtccgagag ctcagctagt cttcttcctc caacccgggc      660

ccctatgtcc acttcaggac agcatgtttg ctgcctccag ggatcctgtg tccccgagct      720

gggaccacct tatattccca gggccggtta atgtggctct ggttctgggt acttttatct      780

gtcccctcca ccccacagtg gggccactag ggacagcgat cgggtacatc gatcgcaggc      840

gcaatcttcg catttctttt ttccagatgg tgagcaaggg cgaggagctg ttcaccgggg      900

tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg      960

gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg     1020

gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct     1080

tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag     1140

gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg     1200

aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca     1260

aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct     1320

atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca     1380

tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg     1440

gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc     1500

ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc     1560

tcggcatgga cgagctgtac aagtaacgcg gccgcctgtg ccttctagtt gccagccatc     1620

tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct     1680

ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg     1740

gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg     1800

ggatgcggtg ggctctatgg gattggtgac agaaaagccc catccttagg cctcctcctt     1860

cctagtctcc tgatattggg tctaaccccc acctcctgtt aggcagattc cttatctggt     1920

gacacacccc catttcctgg agccatctct ctccttgcca gaacctctaa ggtttgctta     1980

cgatggagcc agagaggatc ctgggaggga gagcttggca gggggtggga gggaaggggg     2040

ggatgcgtga cctgcccggt tctcagtggc caccctgcgc taccctctcc cagaacctga     2100

gctgctctga cgcggctgtc tggtgcgttt cactgatcct ggtgctgcag cttccttaca     2160

cttcccaaga ggagaagcag tttggaaaaa caaaatcaga ataagttggt cctgagttct     2220

aactttggct cttcaccttt ctagtcccca atttatattg ttcctccgtg cgtcagtttt     2280

acctgtgaga taaggccagt agccagcccc gtcctggcag ggctgtggtg aggagggggg     2340

tgtccgtgtg gaaaactccc tttgtgagaa tggtgcgtcc taggtgttca ccaggtcgtg     2400

gccgcctcta ctccctttct ctttctccat ccttctttcc ttaaagagtc cccagtgcta     2460

tctgggacat attcctccgc ccagagcagg gtcccgcttc cctaaggccc tgctctgggc     2520

ttctgggttt gagtccttgg caagcccagg agaggcgctc aggcttccct gtcccccttc     2580

ctcgtccacc atctcatgcc cctggctctc ctgccccttc cctacagggg ttcctggctc     2640

tgctcttcag actgagcccc gttcccctgc atccccgttc ccctgcatcc cccttcccct     2700

gcatccccca gaggccccag gccacctact tggcctggac cccacgagag gccaccccag     2760

ccctgtctac caggctgcct tttgggtgga ttctcctcca actgtggggt gactgcttgg     2820

ctatctgcct ggccctggcc attgtcactt tgcgctgccc tcctctcgcc cccgagtgcc     2880

cttgctgtgc cgccggaact ctgccctcta acgctgccgt ctctctcctg agtccggacc     2940

actttgagct ctactggctt ctgcgccgcc tctggcccac tgtttcccct tcccaggcag     3000

gtcc                                                                  3004


<210>  27
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Top strand of AAV1 locus - unedited

<400>  27
ggggccacta gggacaggat tggtgacaga aaagccccat ccttaggcct cctccttcct       60

agtctcctga tattgggtct aa                                                82


<210>  28
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Bottom strand of AAV1 locus - unedited

<400>  28
ccccggtgat ccctgtccta accactgtct tttcggggta ggaatccgga ggaggaagga       60

tcagaggact ataacccaga tt                                                82


<210>  29
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Top strand of AAV1 locus - CBE edited

<400>  29
ggggccacta gggacaggat tggtgacaga aaagccccat ccttaggcct cctccttttt       60

agtctcctga tattgggtct aa                                                82


<210>  30
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Bottom strand of AAV1 locus - CBE edited

<400>  30
ccccggtgat ccctgtccta attactgtct tttcggggta ggaatccgga ggaggaagga       60

tcagaggact ataacccaga tt                                                82


<210>  31
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Top strand of AAV1 locus - ABE edited

<400>  31
ggggccacta gggacaggat tggtgacaga aaagccccat ccttaggcct cctccttcct       60

ggtctcctga tattgggtct aa                                                82


<210>  32
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- Bottom strand of AAV1 locus - ABE edited

<400>  32
ccccggtgat ccctgtcctg gccgctgtct tttcggggta ggaatccgga ggaggaagga       60

tcagaggact ataacccaga tt                                                82


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockin at TRAC locus

<400>  33
agaggatcag ggttaggaca                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockin at TRAC locus

<400>  34
tcagggttct ggatatctgt                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockin at TRAC locus

<400>  35
gctggtacac ggcagggtca                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockin at TRAC locus

<400>  36
ccagaaccct gaccctgccg                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockin at TRAC locus

<400>  37
cctgccgtgt accagctgag                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - gRNA for knockin at TRAC locus

<400>  38
aagtctgtct gcctattcac                                                   20


