               SEQUENCE LISTING

<110> Vaccinvent GmbH
      Universität Ulm

<120> METHOD AND MEANS FOR MODULATING B-CELL MEDIATED IMMUNE RESPONSES

<130> AE1593 PCT BS

<150> EP21 189 996.8
<151> 2021-08-05
<150> PCT/EP2021/052000
<151> 2021-01-28

<160> 48

<170> BiSSAP 1.3.6

<210> 1
<211> 128
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb13 HCVD

<400> 1
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          
Trp Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Val Trp Val 
        35                  40                  45              
Ser Arg Ile Asn Ser Asp Gly Ser Thr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Asp Asp Arg Gly Ile Thr Met Val Arg Gly Ile Ile Leu Phe 
            100                 105                 110         
Gly Phe Leu Asp Leu Trp Gly Arg Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 125             



<210> 2
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb13 HCDR1

<400> 2
Gly Phe Thr Phe Ser Ser Tyr Trp 
1               5               

<210> 3
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb13 HCDR2

<400> 3
Ile Asn Ser Asp Gly Ser Thr Thr 
1               5               

<210> 4
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb13 HCDR3

<400> 4
Ala Arg Asp Asp Arg Gly Ile Thr Met Val Arg Gly Ile Ile Leu Phe 
1               5                   10                  15      
Gly Phe Leu Asp Leu 
            20      

<210> 5
<211> 107
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb13 LCVD

<400> 5
Glu Ile Val Leu Thr Gln Ser Pro Ala Ile Leu Ser Leu Cys Pro Gly 
1               5                   10                  15      
Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Asn Val Ser Ser Tyr 
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              
Ser Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Asn Leu Glu Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser Asn Trp Pro Gln 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         

<210> 6
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb13 LCDR1

<400> 6
Gln Asn Val Ser Ser Tyr 
1               5       

<210> 7
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb13 LCDR3

<400> 7
Gln Gln Arg Ser Asn Trp Pro Gln Thr 
1               5                   

<210> 8
<211> 129
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb48 HCVD

<400> 8
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          
Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Ala Val Ile Trp Tyr Asp Gly Asn Asn Lys Tyr Tyr Thr Asp Ser Val 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Asp Ser Lys Phe Thr Val Val Val Ala Arg Arg Arg Tyr Tyr 
            100                 105                 110         
Tyr Tyr Asp Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser 
        115                 120                 125             
Ser 
    

<210> 9
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb48 HCDR1

<400> 9
Gly Phe Thr Phe Ser Asn Tyr Gly 
1               5               

<210> 10
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb48 HCDR2

<400> 10
Ile Trp Tyr Asp Gly Asn Asn Lys 
1               5               

<210> 11
<211> 22
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb48 HCDR3

<400> 11
Ala Arg Asp Ser Lys Phe Thr Val Val Val Ala Arg Arg Arg Tyr Tyr 
1               5                   10                  15      
Tyr Tyr Asp Met Asp Val 
            20          

<210> 12
<211> 108
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb48 LCVD

<400> 12
Glu Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly 
1               5                   10                  15      
Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Thr Ile Ser Ser Asn 
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              
Tyr Gly Ala Ser Thr Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Glu Ser 
65                  70                  75                  80  
Glu Asp Phe Ala Val Tyr Tyr Cys Gln His Tyr Asn Arg Leu Pro Pro 
                85                  90                  95      
Trp Thr Phe Gly Gln Gly Thr Arg Val Glu Ile Lys 
            100                 105             

<210> 13
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb48 LCDR1

<400> 13
Gln Thr Ile Ser Ser Asn 
1               5       

<210> 14
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb48 LCDR3

<400> 14
Gln His Tyr Asn Arg Leu Pro Pro Trp Thr 
1               5                   10  

<210> 15
<211> 120
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb49 HCVD

<400> 15
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 
            20                  25                  30          
Thr Ile Ser Trp Val Gln Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              
Val Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  
Gln Ser Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  
Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Ser Lys Val Asn Phe Ser Gly Trp Tyr Tyr Tyr Trp Gly Gln 
            100                 105                 110         
Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 

<210> 16
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb49 HCDR1

<400> 16
Gly Gly Thr Phe Ser Ser Tyr Thr 
1               5               

<210> 17
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb49 HCDR2

<400> 17
Ile Ile Pro Ile Phe Gly Thr Ala 
1               5               

<210> 18
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb49 HCDR3

<400> 18
Ala Arg Ser Lys Val Asn Phe Ser Gly Trp Tyr Tyr Tyr 
1               5                   10              

<210> 19
<211> 107
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb49 LCVD

<400> 19
Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      
Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr 
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Ala Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              
Tyr Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser Thr Trp Pro Trp 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         

<210> 20
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb49 LCDR1

<400> 20
Gln Ser Val Ser Ser Tyr 
1               5       

<210> 21
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> mAb49 LCDR3

<400> 21
Gln Gln Arg Ser Thr Trp Pro Trp Thr 
1               5                   

<210> 22
<211> 384
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb13 HCVD

<400> 22
gaggtgcagc tggtggagtc cgggggaggc ttagttcagc ctggggggtc cctgagactc      60

tcctgtgcag cctctggatt caccttcagt agctactgga tgcactgggt ccgccaagct     120

ccagggaagg ggctggtgtg ggtctcacgt attaatagtg atgggagtac cacaaggtac     180

gcggactccg tgaagggccg attcaccatc tccagagaca acgccaagaa tacgctgtat     240

ctgcaaatga acagtctgag agccgaggac acggctgtgt attactgtgc aagagatgat     300

agggggatta ctatggttcg gggaattatt ctattcgggt tcctcgatct ctggggccgt     360

ggcaccctgg tcactgtctc ctca                                            384


<210> 23
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb13 HCDR1

<400> 23
ggattcacct tcagtagcta ctgg                                           24


<210> 24
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb13 HCDR2

<400> 24
attaatagtg atgggagtac caca                                           24


<210> 25
<211> 63
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb13 HCDR3

<400> 25
gcaagagatg atagggggat tactatggtt cggggaatta ttctattcgg gttcctcgat     60

ctc                                                                   63


<210> 26
<211> 322
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb13 LCVD

<400> 26
gaaattgtgt tgacacagtc tccagccatc ctgtctttgt gtccagggga aagagccacc      60

ctctcctgca gggccagtca gaatgttagc agctacttag cctggtacca acagaaacct     120

ggccaggctc ccaggctcct catctctgat gcatccaaca gggccactgg catcccagcc     180

aggttcagtg gcagtgggtc tgggacagac ttcactctca ccatcaacaa cctggagcct     240

gaagattttg cagtttatta ctgtcagcag cgtagcaact ggcctcagac gttcggccaa     300

gggaccaagg tggagatcaa ac                                              322


<210> 27
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb13 LCDR1

<400> 27
cagaatgtta gcagctac                                                  18


<210> 28
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb13 LCDR3

<400> 28
cagcagcgta gcaactggcc tcagacg                                        27


<210> 29
<211> 387
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb48 HCVD

<400> 29
caggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc      60

tcctgtgcag cgtctggatt caccttcagt aactatggta tgcactgggt ccgccaggct     120

ccaggcaagg ggctggagtg ggtggcagtt atatggtatg atggaaataa taaatactat     180

acagactccg tgaagggccg attcaccatc tccagagaca attccaagaa caccctatat     240

ctgcaaatga acagcctgag agccgaggac acggctgtgt attactgtgc gagagattcc     300

aaatttacag tagtggttgc acggcgacgg tactactact acgatatgga cgtctggggc     360

caagggacca cggtcaccgt ctcctca                                         387


<210> 30
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb48 HCDR1

<400> 30
ggattcacct tcagtaacta tggt                                           24


<210> 31
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb48 HCDR2

<400> 31
atatggtatg atggaaataa taaa                                           24


<210> 32
<211> 66
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb48 HCDR3

<400> 32
gcgagagatt ccaaatttac agtagtggtt gcacggcgac ggtactacta ctacgatatg     60

gacgtc                                                                66


<210> 33
<211> 325
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb48 LCVD

<400> 33
gaaatagtga tgacgcagtc tccagccacc ctgtctgtgt ctccagggga aagagccacc      60

ctctcctgca gggccagtca gactattagc agcaacttag cctggtacca gcagaaacct     120

ggccaggctc ccaggctcct catctatggt gcatccacca gggccactgg tatcccagct     180

aggttcagtg gcagtgggtc tgggacagag ttcactctca ccatcagcag cctggagtct     240

gaagattttg cagtttatta ctgtcaacac tataataggc tgcctccgtg gacgttcggc     300

caagggacca gggtggaaat caaac                                           325


<210> 34
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb48 LCDR1

<400> 34
cagactatta gcagcaac                                                  18


<210> 35
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb48 LCDR3

<400> 35
caacactata ataggctgcc tccgtggacg                                     30


<210> 36
<211> 360
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb49 HCVD

<400> 36
caggtgcagc tggtgcagtc tggggctgag gtgaagaagc ctgggtcctc ggtgaaggtc      60

tcctgcaagg cttctggagg caccttcagc agctatacta tcagctgggt gcaacaggcc     120

cctggacaag ggcttgagtg gatggtaggg atcatcccta tctttggtac agcaaactac     180

gcacagaagt tccagagcag agtcacgatt accgcggacg aatccacgag cacagcctac     240

atggagctga gcagcctgag atctgaggac acggccgtgt attactgtgc gagatcgaaa     300

gttaatttca gtggctggta ctactactgg ggccagggaa ccctggtcac cgtctcctca     360


<210> 37
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb49 HCDR1

<400> 37
ggaggcacct tcagcagcta tact                                           24


<210> 38
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb49 HCDR2

<400> 38
atcatcccta tctttggtac agca                                           24


<210> 39
<211> 39
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb49 HCDR3

<400> 39
gcgagatcga aagttaattt cagtggctgg tactactac                            39


<210> 40
<211> 322
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb49 LCVD

<400> 40
gaaattgtgt tgacacagtc tccagccacc ctgtctttgt ctccagggga aagagccacc      60

ctctcctgca gggccagtca gagtgttagc agctacttag cctggtacca acagaaagct     120

ggccaggctc ccaggctcct catctatgat gcatccaaca gggccactgg catcccagcc     180

aggttcagtg gcagtgggtc tgggacagac ttcactctca ccatcagcag gctagagcct     240

gaagattttg cagtttatta ctgtcagcag cgtagcacct ggccgtggac gttcggccaa     300

gggaccaagg tggaaatcaa ac                                              322


<210> 41
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb49 LCDR1

<400> 41
cagagtgtta gcagctac                                                  18


<210> 42
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> mAb49 LCDR3

<400> 42
cagcagcgta gcacctggcc gtggacg                                        27


<210> 43
<211> 26
<212> PRT
<213> Viruses


<220> 
<223> SARS-CoV-2 #1

<400> 43
Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln 
1               5                   10                  15      
Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr 
            20                  25      

<210> 44
<211> 30
<212> PRT
<213> Viruses


<220> 
<223> SARS-CoV-2 #2

<400> 44
Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
1               5                   10                  15      
Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr 
            20                  25                  30  

<210> 45
<211> 31
<212> PRT
<213> Homo sapiens


<220> 
<223> C-Peptide

<400> 45
Glu Ala Glu Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly Gly Pro 
1               5                   10                  15      
Gly Ala Gly Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu Gln 
            20                  25                  30      

<210> 46
<211> 29
<212> PRT
<213> Mus musculus


<220> 
<223> C-Peptide

<400> 46
Glu Val Glu Asp Pro Gln Val Glu Gln Leu Glu Leu Gly Gly Ser Pro 
1               5                   10                  15      
Gly Asp Leu Gln Thr Leu Ala Leu Glu Val Ala Arg Gln 
            20                  25                  

<210> 47
<211> 21
<212> PRT
<213> Homo sapiens


<220> 
<223> Insulin-A chain

<400> 47
Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      
Glu Asn Tyr Cys Asn 
            20      

<210> 48
<211> 21
<212> PRT
<213> Mus musculus


<220> 
<223> Insulin-A chain

<400> 48
Gly Ile Val Asp Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      
Glu Asn Tyr Cys Asn 
            20      

