                         SEQUENCE LISTING

<110>  Wageningen Universiteit
 
<120>  CRISPR-associated base-editing of the complementary strand

<130>  P129418PC00

<140>  PCT/NL2022/050042
<141>  2022-01-28

<150>  EP 21154421.8
<151>  2021-01-29

<150>  EP 21166717.5
<151>  2021-04-01

<160>  229   

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  c-myc domain tag

<400>  1

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  2
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hemagglutinin tag

<400>  2

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  3
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal loop domain

<400>  3

Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Ile Asn 
1               5                   10          


<210>  4
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal loop domain in inverted orientation

<400>  4

Asn Ile Ser Ser Gln Ala Lys Lys Gln Ala Asn Phe 
1               5                   10          


<210>  5
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  loop region

<400>  5

Tyr Arg Met Pro Arg Gln 
1               5       


<210>  6
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence

<400>  6

Asn Ile Ser Ser Gln Ala Lys Lys Gln Ala Asn Phe Tyr Arg Met Pro 
1               5                   10                  15      


Arg Gln 
        


<210>  7
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C motif sequence


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (8)..(9)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (11)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  7
cnncnncnnc nncnncnnc                                                    19


<210>  8
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG14064

<400>  8
gatgtcctcc tgagctcgc                                                    19


<210>  9
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG14065

<400>  9
aagcttggct gttttggcg                                                    19


<210>  10
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19000

<400>  10
acgagctgta caagactagt cccaagaaga aacggaaagt                             40


<210>  11
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19001

<400>  11
cgccaaaaca gccaagcttt tagactttcc tcttcttctt g                           41


<210>  12
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19002

<400>  12
gcgagctcag gaggaccata tggtgtctaa gggcgaagag                             40


<210>  13
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19003

<400>  13
actagtcttg tacagctcgt ccatgc                                            26


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19102

<400>  14
cccaagaaga aacggaaagt                                                   20


<210>  15
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19104

<400>  15
cactttccgt ttcttcttgg gaagtcttcg ttaagcaccg gtggagtg                    48


<210>  16
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19118

<400>  16
gcgagctcag gaggacatct tgtcttcttg acaattaatc atcggctc                    48


<210>  17
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG14065

<400>  17
aagcttggct gttttggcg                                                    19


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15295

<400>  18
ggggttcgag ggggcagttg                                                   20


<210>  19
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15296

<400>  19
caactgcccc ctcgaacccc ggtggaggag gttctggag                              39


<210>  20
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15297

<400>  20
cgccaaaaca gccaagcttt tatgcaacca gtcctagcat c                           41


<210>  21
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20896

<400>  21
acacgctctt ctatgaccga cgctgagtac gtg                                    33


<210>  22
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20897

<400>  22
acacgctctt ctggggttcg agggggcagt tg                                     32


<210>  23
<211>  115
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20898

<400>  23
acacgctctt ctcccccctg caccagctcc tgctccagca cctgctccag cacctgctcc       60

agctccagca cctgcacctg caccagctcc agcacctgct ccagctcctg ctcct           115


<210>  24
<211>  115
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20899

<400>  24
acacgctctt cttcatagga gcaggagctg gagcaggtgc tggagctggt gcaggtgcag       60

gtgctggagc tggagcaggt gctggagcag gtgctggagc aggagctggt gcagg           115


<210>  25
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20900

<400>  25
acacgctctt ctcccctccg gagactataa ggaccac                                37


<210>  26
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20901

<400>  26
acacgctctt cttcatggac tcgagcctag acttatc                                37


<210>  27
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20902

<400>  27
acacgctctt ctccccggtg gaggaggttc tggagg                                 36


<210>  28
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20903

<400>  28
acacgctctt cttcatatac ttctccacgt aagggac                                37


<210>  29
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20904

<400>  29
acacgctctt cttcattccg gactcgagcc tagactt                                37


<210>  30
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20905

<400>  30
acacgaagac ttcatcatga caacaatgac agtacatac                              39


<210>  31
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20906

<400>  31
acacgaagac tttcatccta gacttatcgt catcg                                  35


<210>  32
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20907

<400>  32
acacgaagac ttatgaatga gctcagagac tggccc                                 36


<210>  33
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20908

<400>  33
acacgaagac ttcatcatga caacaatgac agtacatac                              39


<210>  34
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20909

<400>  34
acacgaagac aatgggaaca gcaggactct tagtgg                                 36


<210>  35
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21058

<400>  35
caaagacgat gacgataagt ctaggatgac agacgccgag tacgtg                      46


<210>  36
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21059

<400>  36
ctccacctcc agaacctcct ccacccggat tactcggtgc cgtgg                       45


<210>  37
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21060

<400>  37
ccacggcacc gagtaatccg ggtggaggag gttctggagg                             40


<210>  38
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21061

<400>  38
cacgtactcg gcgtctgtca t                                                 21


<210>  39
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19700

<400>  39
acacgaagac ttcatcatga ccaccatgac cgtgcac                                37


<210>  40
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19702

<400>  40
acacgaagac aactgaggtc ccgggagtct cgctgccgcc ggattactcg gtgccgtgg        59


<210>  41
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19703

<400>  41
acacgaagac tttcagagtc cgccacaccc gaaagtatga gctcagagac tggcccag         58


<210>  42
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19704

<400>  42
acacgaagac aatgggtttc aacccggtgg cccag                                  35


<210>  43
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19709

<400>  43
acacgaagac tttcagagtc cgccacaccc gaaagtatga cagacgccga gtacgtg          57


<210>  44
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19710

<400>  44
acacgaagac aatgggacca acggctggag acttagtg                               38


<210>  45
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21160

<400>  45
agcggcagcg agactccc                                                     18


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21161

<400>  46
cggattactc ggtgccgtgg                                                   20


<210>  47
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20003

<400>  47
agacttgaat tagatggtga tgtt                                              24


<210>  48
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20004

<400>  48
acacaacatc accatctaat tcaa                                              24


<210>  49
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15892

<400>  49
gatcggtacc cattaaccta taaaaatagg cgtc                                   34


<210>  50
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15893

<400>  50
caatttttaa tgaacggacg tcg                                               23


<210>  51
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15894

<400>  51
cttattcatt aaaaattgaa ttgaggtac                                         29


<210>  52
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15895

<400>  52
ctcaattcaa tttttaatga ataagacgt                                         29


<210>  53
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15896

<400>  53
cttacccccc aaaaattgaa ttgaggtac                                         29


<210>  54
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15897

<400>  54
ctcaattcaa tttttggggg gtaagacgt                                         29


<210>  55
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15898

<400>  55
cttattcccc cccaattgaa ttgaggtac                                         29


<210>  56
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15899

<400>  56
ctcaattcaa ttggggggga ataagacgt                                         29


<210>  57
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15900

<400>  57
cttattcatt cccccctgaa ttgaggtac                                         29


<210>  58
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15901

<400>  58
ctcaattcag gggggaatga ataagacgt                                         29


<210>  59
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15902

<400>  59
cttattcatt aaacccccca ttgaggtac                                         29


<210>  60
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15903

<400>  60
ctcaatgggg ggtttaatga ataagacgt                                         29


<210>  61
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15904

<400>  61
cttattcatt aaaaatcccc ccgaggtac                                         29


<210>  62
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15905

<400>  62
ctcgggggga tttttaatga ataagacgt                                         29


<210>  63
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15906

<400>  63
cttattcatt aaaaattgac ccccggtac                                         29


<210>  64
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15907

<400>  64
cgggggtcaa tttttaatga ataagacgt                                         29


<210>  65
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15908

<400>  65
agacttcatt aaaaattgaa ttga                                              24


<210>  66
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15909

<400>  66
acactcaatt caatttttaa tgaa                                              24


<210>  67
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15910

<400>  67
agaccccccc aaaaattgaa ttga                                              24


<210>  68
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15911

<400>  68
acactcaatt caatttttgg gggg                                              24


<210>  69
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15912

<400>  69
agacttcccc cccaattgaa ttga                                              24


<210>  70
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15913

<400>  70
acactcaatt caattggggg ggaa                                              24


<210>  71
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15914

<400>  71
agacttcatt cccccctgaa ttga                                              24


<210>  72
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15915

<400>  72
acactcaatt caggggggaa tgaa                                              24


<210>  73
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15916

<400>  73
agacttcatt aaacccccca ttga                                              24


<210>  74
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15917

<400>  74
acactcaatg gggggtttaa tgaa                                              24


<210>  75
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15918

<400>  75
agacttcatt aaaaatcccc ccga                                              24


<210>  76
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15919

<400>  76
acactcgggg ggatttttaa tgaa                                              24


<210>  77
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15920

<400>  77
agacttcatt aaaaattgac cccc                                              24


<210>  78
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15921

<400>  78
acacgggggt caatttttaa tgaa                                              24


<210>  79
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18520

<400>  79
agacttcatt aaaaattg                                                     18


<210>  80
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18521

<400>  80
acaccaattt ttaatgaa                                                     18


<210>  81
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18522

<400>  81
agacttcatt aaaaattga                                                    19


<210>  82
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18523

<400>  82
acactcaatt tttaatgaa                                                    19


<210>  83
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18524

<400>  83
agacttcatt aaaaattgaa                                                   20


<210>  84
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18525

<400>  84
acacttcaat ttttaatgaa                                                   20


<210>  85
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18526

<400>  85
agacttcatt aaaaattgaa t                                                 21


<210>  86
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18527

<400>  86
acacattcaa tttttaatga a                                                 21


<210>  87
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18528

<400>  87
agacttcatt aaaccccc                                                     18


<210>  88
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18529

<400>  88
acacgggggt ttaatgaa                                                     18


<210>  89
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18530

<400>  89
agacttcatt aaacccccc                                                    19


<210>  90
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18531

<400>  90
acacgggggg tttaatgaa                                                    19


<210>  91
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18532

<400>  91
agacttcatt aaacccccca                                                   20


<210>  92
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18533

<400>  92
acactggggg gtttaatgaa                                                   20


<210>  93
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18534

<400>  93
agacttcatt aaacccccca t                                                 21


<210>  94
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18535

<400>  94
acacatgggg ggtttaatga a                                                 21


<210>  95
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18536

<400>  95
agacttcatt aaaaatcc                                                     18


<210>  96
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18537

<400>  96
acacggattt ttaatgaa                                                     18


<210>  97
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18538

<400>  97
agacttcatt aaaaatccc                                                    19


<210>  98
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18539

<400>  98
acacgggatt tttaatgaa                                                    19


<210>  99
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18540

<400>  99
agacttcatt aaaaatcccc                                                   20


<210>  100
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18541

<400>  100
acacggggat ttttaatgaa                                                   20


<210>  101
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18542

<400>  101
agacttcatt aaaaatcccc c                                                 21


<210>  102
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18543

<400>  102
acacggggga tttttaatga a                                                 21


<210>  103
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18544

<400>  103
agacttcatt aaaaattgac                                                   20


<210>  104
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18545

<400>  104
acacgtcaat ttttaatgaa                                                   20


<210>  105
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18546

<400>  105
agacttcatt aaaaattgac c                                                 21


<210>  106
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18547

<400>  106
acacggtcaa tttttaatga a                                                 21


<210>  107
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20162

<400>  107
agaccatcgt caacgtcagc atct                                              24


<210>  108
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20163

<400>  108
acacagatgc tgacgttgac gatg                                              24


<210>  109
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20164

<400>  109
agacactgct acagctacga cttc                                              24


<210>  110
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20165

<400>  110
acacgaagtc gtagctgtag cagt                                              24


<210>  111
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20166

<400>  111
agacatcgtc aacgtcagca tcta                                              24


<210>  112
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20167

<400>  112
acactagatg ctgacgttga cgat                                              24


<210>  113
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20168

<400>  113
cttacatcgt caacgtcagc atctggtac                                         29


<210>  114
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20169

<400>  114
cagatgctga cgttgacgat gtaagacgt                                         29


<210>  115
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20170

<400>  115
cttaactgct acagctacga cttcggtac                                         29


<210>  116
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20171

<400>  116
cgaagtcgta gctgtagcag ttaagacgt                                         29


<210>  117
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20172

<400>  117
cttaatcgtc aacgtcagca tctaggtac                                         29


<210>  118
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20173

<400>  118
ctagatgctg acgttgacga ttaagacgt                                         29


<210>  119
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15302

<400>  119
ggtgatgtcg gcgatatagg                                                   20


<210>  120
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG15034

<400>  120
gatttaatct gtatcaggct gaaaatc                                           27


<210>  121
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18776

<400>  121
cttgatcaga tcttgatccc ctg                                               23


<210>  122
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18664

<400>  122
acaccttcac cctctccact gaca                                              24


<210>  123
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG11655

<400>  123
cgttggatct ctcttctaag                                                   20


<210>  124
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG11656

<400>  124
cacctgtaag cgttgatttc                                                   20


<210>  125
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG12945

<400>  125
gatcatttat ctttcactgc ggag                                              24


<210>  126
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG16493

<400>  126
tttttttgtt ttttatgtct tatcgtgac                                         29


<210>  127
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG14031

<400>  127
tactgttgta gatttttttt gttttttatg tcttatcgtg acgcag                      46


<210>  128
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG14032

<400>  128
agacataaaa aacaaaaaaa atctacaaca gtagaaattt ccacacca                    48


<210>  129
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG16236

<400>  129
tctcccacca gcaaagcctg aatttctact gttgtagatt ttttttgtt                   49


<210>  130
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG14555

<400>  130
atctacaaca gtagaaattg atcat                                             25


<210>  131
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG16700

<400>  131
cgccggcgcg atgccgccaa aacgtcttgg                                        30


<210>  132
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG14156

<400>  132
ggtgatgtcg gcgatatagg                                                   20


<210>  133
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG17373

<400>  133
cactatagca atctggctat atg                                               23


<210>  134
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG17374

<400>  134
aaacgcctgt gggtgtggta ctggatatgc aaagcgattg gaagtcgctt gactcctctg       60

ccgtcattcc                                                              70


<210>  135
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG17375

<400>  135
ttgcccatcg aacgtacaag tactcctctg ttctctcctt cctttgcttt aagcgttgaa       60

gtttcctctt tg                                                           72


<210>  136
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG17376

<400>  136
tgtcaactgg agagctatcg                                                   20


<210>  137
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18470

<400>  137
agacgcgttg agttggtgta tggc                                              24


<210>  138
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG18471

<400>  138
acacgccata caccaactca acgc                                              24


<210>  139
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19774

<400>  139
aagcgacttc caatcgcttt gcatatccag taccacaccc acaggcgttt ttttcttttt       60

ttgcggtcac cccc                                                         74


<210>  140
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19775

<400>  140
tgaaaagttc ttctcctttg ctcatttttg ataagtattt aagcgagtga ctgaa            55


<210>  141
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19776

<400>  141
tcactcgctt aaatacttat caaaaatgag caaaggagaa gaacttttca ctgg             54


<210>  142
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG17378

<400>  142
aagcaaagga aggagagaac agaggagtac ttgtacgttc gatgggcaac ttcgagcgtc       60

ccaaaacctt c                                                            71


<210>  143
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20455

<400>  143
accgctgctc cccgtagc                                                     18


<210>  144
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG13880

<400>  144
tgcttcattt tgtagaacaa aaatg                                             25


<210>  145
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19061

<400>  145
agacaccgtg cataccgctg ctccccgtag c                                      31


<210>  146
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG19062

<400>  146
acacgctacg gggagcagcg gtatgcacgg t                                      31


<210>  147
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG13879

<400>  147
catttttgtt ctacaaaatg aagca                                             25


<210>  148
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20456

<400>  148
caacttatat cgtatggagc agcggtatgc acggt                                  35


<210>  149
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20457

<400>  149
gctacgggga gcagcggtaa aaaaaccccg ccgaagcg                               38


<210>  150
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20239

<400>  150
ggacatcacc tctcacaacg aagatt                                            26


<210>  151
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20240

<400>  151
aatcttcgtt gtgagaggtg atgtcc                                            26


<210>  152
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20514

<400>  152
ccgctgctcc atacgatata agttgtaatt cggtaccccg                             40


<210>  153
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20455

<400>  153
accgctgctc cccgtagc                                                     18


<210>  154
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG13880

<400>  154
tgcttcattt tgtagaacaa aaatg                                             25


<210>  155
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG13879

<400>  155
catttttgtt ctacaaaatg aagca                                             25


<210>  156
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20456

<400>  156
caacttatat cgtatggagc agcggtatgc acggt                                  35


<210>  157
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20457

<400>  157
gctacgggga gcagcggtaa aaaaaccccg ccgaagcg                               38


<210>  158
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20239

<400>  158
ggacatcacc tctcacaacg aagatt                                            26


<210>  159
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20240

<400>  159
aatcttcgtt gtgagaggtg atgtcc                                            26


<210>  160
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20514

<400>  160
ccgctgctcc atacgatata agttgtaatt cggtaccccg                             40


<210>  161
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20383

<400>  161
cggatcgatg tacacaaccg actgcaccca aacgaacaca aatcttagca tcatagcttc       60

aaaatgtttc tact                                                         74


<210>  162
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21049

<400>  162
gccggtacct cctccaccca ccttcctctt cttcttgggc tttttctttt ttgcctggc        59


<210>  163
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21050

<400>  163
ggccaggcaa aaaagaaaaa gcccaagaag aagaggaagg t                           41


<210>  164
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21051

<400>  164
gggcgtgaat gtaagcgtga cataactaat ttatagcatc ttgatcttgt tc               52


<210>  165
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21052

<400>  165
aatggagaga acaagatcaa gatgctataa attagttatg tcacgcttac at               52


<210>  166
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20390

<400>  166
caacaggagg cggatggata tactgtggtc tggaagatgc cggaaagcgt gatcccaata       60

caacagatca c                                                            71


<210>  167
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20384

<400>  167
cgatagaacc taaataaaac gagctcaaat taaagccttc gagcg                       45


<210>  168
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20385

<400>  168
agaagatttc tcttcaatct cct                                               23


<210>  169
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20386

<400>  169
tgctaagatt tgtgttcgtt tgggtgcagt cggttgtgta catcgatccg cccttatcaa       60

ggatacctgg                                                              70


<210>  170
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20387

<400>  170
acgctttccg gcatcttcca gaccacagta tatccatccg cctcctgttg ggcgattaca       60

caagcg                                                                  66


<210>  171
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20388

<400>  171
tctcctcttc gatgacc                                                      17


<210>  172
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG20389

<400>  172
tgggacgctc gaaggcttta atttgagctc gttttattta ggttctatc                   49


<210>  173
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21235

<400>  173
aagatggttc cgttcaacta gtg                                               23


<210>  174
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21236

<400>  174
tagttgaacg gaaccatctt gtc                                               23


<210>  175
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21237

<400>  175
cccaaagtgt tcctgtggaa gtg                                               23


<210>  176
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21238

<400>  176
ttccacagga acactttggg gtc                                               23


<210>  177
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21239

<400>  177
tgcaaatgcc tagaggtgtt gtg                                               23


<210>  178
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21240

<400>  178
aacacctcta ggcatttgca gtc                                               23


<210>  179
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21241

<400>  179
atacaagaca aatatattca gtg                                               23


<210>  180
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21242

<400>  180
tgaatatatt tgtcttgtat gtc                                               23


<210>  181
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21243

<400>  181
aagcaagaag aagaagttct gtg                                               23


<210>  182
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide BG21244

<400>  182
agaacttctt cttcttgctt gtc                                               23


<210>  183
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A motif sequence


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (8)..(9)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (11)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  183
annannanna nnannanna                                                    19


<210>  184
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G motif sequence


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (8)..(9)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (11)..(12)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  n is a, c, g, or t

<400>  184
gnngnngnng nngnngnng                                                    19


<210>  185
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C-tile target: wt

<400>  185
ttcattaaaa attgaattga                                                   20


<210>  186
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C-tile target: C-tile 1

<400>  186
ccccccaaaa attgaattga                                                   20


<210>  187
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C-tile target: C-tile 2

<400>  187
ttccccccca attgaattga                                                   20


<210>  188
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C-tile target: C-tile 3

<400>  188
ttcattcccc cctgaattga                                                   20


<210>  189
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C-tile target: C-tile 4

<400>  189
ttcattaaac cccccattga                                                   20


<210>  190
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C-tile target: C-tile 5

<400>  190
ttcattaaaa atccccccga                                                   20


<210>  191
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C-tile target: C-tile 6

<400>  191
ttcattaaaa attgaccccc                                                   20


<210>  192
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Binding sequence

<400>  192
ttgaattaga tggtgatgtt                                                   20


<210>  193
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C1 motif

<400>  193
catcgtcaac gtcagcatct                                                   20


<210>  194
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C2 motif

<400>  194
actgctacag ctacgacttc                                                   20


<210>  195
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C3 motif

<400>  195
atcgtcaacg tcagcatcta                                                   20


<210>  196
<211>  7
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  196

Thr Gln Ser Val Pro Val Glu 
1               5           


<210>  197
<211>  7
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  197

Val Gln Met Pro Arg Gly Val 
1               5           


<210>  198
<211>  7
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  198

Lys Gln Glu Glu Glu Val Leu 
1               5           


<210>  199
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Base edited ADE2_1

<400>  199

Ser Val Pro Val Glu 
1               5   


<210>  200
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Base edited ADE2_2

<400>  200

Met Pro Arg Gly Val 
1               5   


<210>  201
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Base edited ADE2_3

<400>  201

Glu Glu Glu Val Leu 
1               5   


<210>  202
<211>  20
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  202
cccaaagtgt tcctgtggaa                                                   20


<210>  203
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Base edited ADE2_1

<400>  203
cctaaagtgt tcctgtggaa                                                   20


<210>  204
<211>  20
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  204
tgcaaatgcc tagaggtgtt                                                   20


<210>  205
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Base edited ADE2_2

<400>  205
tgtaaatgcc tagaggtgtt                                                   20


<210>  206
<211>  20
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  206
aagcaagaag aagaagttct                                                   20


<210>  207
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Base edited ADE2_3

<400>  207
aagtaagaag aagaagttct                                                   20


<210>  208
<211>  166
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TadA8e

<400>  208

Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu Thr 
1               5                   10                  15      


Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala Val 
            20                  25                  30          


Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala Ile 
        35                  40                  45              


Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg Gln 
    50                  55                  60                  


Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr 
65                  70                  75                  80  


Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His Ser 
                85                  90                  95      


Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ser Lys Arg Gly Ala 
            100                 105                 110         


Ala Gly Ser Leu Met Asn Val Leu Asn Tyr Pro Gly Met Asn His Arg 
        115                 120                 125             


Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu 
    130                 135                 140                 


Cys Asp Phe Tyr Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys Lys 
145                 150                 155                 160 


Ala Gln Ser Ser Ile Asn 
                165     


<210>  209
<211>  184
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TadA8e_eng

<400>  209

Asn Ile Ser Ser Gln Ala Lys Lys Gln Ala Asn Phe Tyr Arg Met Pro 
1               5                   10                  15      


Arg Gln Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala 
            20                  25                  30          


Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly 
        35                  40                  45              


Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg 
    50                  55                  60                  


Ala Ile Gly Leu His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu 
65                  70                  75                  80  


Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr 
                85                  90                  95      


Leu Tyr Val Thr Phe Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile 
            100                 105                 110         


His Ser Arg Ile Gly Arg Val Val Phe Gly Val Arg Asn Ser Lys Arg 
        115                 120                 125             


Gly Ala Ala Gly Ser Leu Met Asn Val Leu Asn Tyr Pro Gly Met Asn 
    130                 135                 140                 


His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala 
145                 150                 155                 160 


Leu Leu Cys Asp Phe Tyr Arg Met Pro Arg Gln Val Phe Asn Ala Gln 
                165                 170                 175     


Lys Lys Ala Gln Ser Ser Ile Asn 
            180                 


<210>  210
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A1 motif

<400>  210
accactatca gcacgagtat ggt                                               23


<210>  211
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A2 motif

<400>  211
cacaattacg actattagga ggt                                               23


<210>  212
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A3 motif

<400>  212
ccaatattag catgatcagc ggt                                               23


<210>  213
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C1 motif

<400>  213
catcgtcaac gtcagcatct ggt                                               23


<210>  214
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C2 motif

<400>  214
actcctacag ctacgacttc ggt                                               23


<210>  215
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C3 motif

<400>  215
atcctcaacg tcagcatcta ggt                                               23


<210>  216
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C1 motif

<400>  216
catcgtcaac gtcagcatct                                                   20


<210>  217
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C2 motif

<400>  217
actcctacag ctacgacttc                                                   20


<210>  218
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C3 motif

<400>  218
atcctcaacg tcagcatcta                                                   20


<210>  219
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A1 motif

<400>  219
accactatca gcacgagtat                                                   20


<210>  220
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A2 motif

<400>  220
cacaattacg actattagga                                                   20


<210>  221
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A3 motif

<400>  221
ccaatattag catgatcagc                                                   20


<210>  222
<211>  849
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas12f1 CBE1

<400>  222

Met Ile Lys Val Tyr Arg Tyr Glu Ile Val Lys Pro Leu Asp Leu Asp 
1               5                   10                  15      


Trp Lys Glu Phe Gly Thr Ile Leu Arg Gln Leu Gln Gln Glu Thr Arg 
            20                  25                  30          


Phe Ala Leu Asn Lys Ala Thr Gln Leu Ala Trp Glu Trp Met Gly Phe 
        35                  40                  45              


Ser Ser Asp Tyr Lys Asp Asn His Gly Glu Tyr Pro Lys Ser Lys Asp 
    50                  55                  60                  


Ile Leu Gly Tyr Thr Asn Val His Gly Tyr Ala Tyr His Thr Ile Lys 
65                  70                  75                  80  


Thr Lys Ala Tyr Arg Leu Asn Ser Gly Asn Leu Ser Gln Thr Ile Lys 
                85                  90                  95      


Arg Ala Thr Asp Arg Phe Lys Ala Tyr Gln Lys Glu Ile Leu Arg Gly 
            100                 105                 110         


Asp Met Ser Ile Pro Ser Tyr Lys Arg Asp Ile Pro Leu Asp Leu Ile 
        115                 120                 125             


Lys Glu Asn Ile Ser Val Asn Arg Met Asn His Gly Asp Tyr Ile Ala 
    130                 135                 140                 


Ser Leu Ser Leu Leu Ser Asn Pro Ala Lys Gln Glu Met Asn Val Lys 
145                 150                 155                 160 


Arg Lys Ile Ser Val Ile Ile Ile Val Arg Gly Ala Gly Lys Thr Ile 
                165                 170                 175     


Met Asp Arg Ile Leu Ser Gly Glu Tyr Gln Val Ser Ala Ser Gln Ile 
            180                 185                 190         


Ile His Asp Asp Arg Lys Asn Lys Trp Tyr Leu Asn Ile Ser Tyr Asp 
        195                 200                 205             


Phe Glu Pro Gln Thr Arg Val Leu Asp Leu Asn Lys Ile Met Gly Ile 
    210                 215                 220                 


Ala Leu Gly Val Ala Val Ala Val Tyr Met Ala Phe Gln His Thr Pro 
225                 230                 235                 240 


Ala Arg Tyr Lys Leu Glu Gly Gly Glu Ile Glu Asn Phe Arg Arg Gln 
                245                 250                 255     


Val Glu Ser Arg Arg Ile Ser Met Leu Arg Gln Gly Lys Tyr Ala Gly 
            260                 265                 270         


Gly Ala Arg Gly Gly His Gly Arg Asp Lys Arg Ile Lys Pro Ile Glu 
        275                 280                 285             


Gln Leu Arg Asp Lys Ile Ala Asn Phe Arg Asp Thr Thr Asn His Arg 
    290                 295                 300                 


Tyr Ser Arg Tyr Ile Val Asp Met Ala Ile Lys Glu Gly Cys Gly Thr 
305                 310                 315                 320 


Ile Gln Met Glu Asp Leu Thr Asn Ile Arg Asp Ile Gly Ser Arg Phe 
                325                 330                 335     


Leu Gln Asn Trp Thr Tyr Tyr Asp Leu Gln Gln Lys Ile Ile Tyr Lys 
            340                 345                 350         


Ala Glu Glu Ala Gly Ile Lys Val Ile Lys Ile Asp Pro Gln Tyr Thr 
        355                 360                 365             


Ser Gln Arg Cys Ser Glu Cys Gly Asn Ile Asp Ser Gly Asn Arg Ile 
    370                 375                 380                 


Gly Gln Ala Ile Phe Lys Cys Arg Ala Cys Gly Tyr Glu Ala Asn Ala 
385                 390                 395                 400 


Asp Tyr Asn Ala Ala Arg Asn Ile Ala Ile Pro Asn Ile Asp Lys Ile 
                405                 410                 415     


Ile Ala Glu Ser Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            420                 425                 430         


Ala Glu Tyr Val Arg Ala Leu Phe Asp Phe Asn Gly Asn Asp Glu Glu 
        435                 440                 445             


Asp Leu Pro Phe Lys Lys Gly Asp Ile Leu Arg Ile Arg Asp Lys Pro 
    450                 455                 460                 


Glu Glu Gln Trp Trp Asn Ala Glu Asp Ser Glu Gly Lys Arg Gly Met 
465                 470                 475                 480 


Ile Pro Val Pro Tyr Val Glu Lys Tyr Ser Gly Asp Tyr Lys Asp His 
                485                 490                 495     


Asp Gly Asp Tyr Lys Asp His Asp Ile Asp Tyr Lys Asp Asp Asp Asp 
            500                 505                 510         


Lys Ser Arg Met Ser Ser Glu Thr Gly Pro Val Ala Val Asp Pro Thr 
        515                 520                 525             


Leu Arg Arg Arg Ile Glu Pro His Glu Phe Glu Val Phe Phe Asp Pro 
    530                 535                 540                 


Arg Glu Leu Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile Asn Trp Gly 
545                 550                 555                 560 


Gly Arg His Ser Ile Trp Arg His Thr Ser Gln Asn Thr Asn Lys His 
                565                 570                 575     


Val Glu Val Asn Phe Ile Glu Lys Phe Thr Thr Glu Arg Tyr Phe Cys 
            580                 585                 590         


Pro Asn Thr Arg Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys 
        595                 600                 605             


Gly Glu Cys Ser Arg Ala Ile Thr Glu Phe Leu Ser Arg Tyr Pro His 
    610                 615                 620                 


Val Thr Leu Phe Ile Tyr Ile Ala Arg Leu Tyr His His Ala Asp Pro 
625                 630                 635                 640 


Arg Asn Arg Gln Gly Leu Arg Asp Leu Ile Ser Ser Gly Val Thr Ile 
                645                 650                 655     


Gln Ile Met Thr Glu Gln Glu Ser Gly Tyr Cys Trp Arg Asn Phe Val 
            660                 665                 670         


Asn Tyr Ser Pro Ser Asn Glu Ala His Trp Pro Arg Tyr Pro His Leu 
        675                 680                 685             


Trp Val Arg Leu Tyr Val Leu Glu Leu Tyr Cys Ile Ile Leu Gly Leu 
    690                 695                 700                 


Pro Pro Cys Leu Asn Ile Leu Arg Arg Lys Gln Pro Gln Leu Thr Phe 
705                 710                 715                 720 


Phe Thr Ile Ala Leu Gln Ser Cys His Tyr Gln Arg Leu Pro Pro His 
                725                 730                 735     


Ile Leu Trp Ala Thr Gly Leu Lys Pro Lys Lys Lys Arg Lys Val Ser 
            740                 745                 750         


Gly Gly Ser Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly Lys 
        755                 760                 765             


Gln Leu Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val Glu 
    770                 775                 780                 


Glu Val Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr Ala 
785                 790                 795                 800 


Tyr Asp Glu Ser Thr Asp Glu Asn Val Met Leu Leu Thr Ser Asp Ala 
                805                 810                 815     


Pro Glu Tyr Lys Pro Trp Ala Leu Val Ile Gln Asp Ser Asn Gly Glu 
            820                 825                 830         


Asn Lys Ile Lys Met Leu Ser Gly Gly Ser Pro Lys Lys Lys Arg Lys 
        835                 840                 845             


Val 
    


<210>  223
<211>  772
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas12f1 CBE2

<400>  223

Met Ile Lys Val Tyr Arg Tyr Glu Ile Val Lys Pro Leu Asp Leu Asp 
1               5                   10                  15      


Trp Lys Glu Phe Gly Thr Ile Leu Arg Gln Leu Gln Gln Glu Thr Arg 
            20                  25                  30          


Phe Ala Leu Asn Lys Ala Thr Gln Leu Ala Trp Glu Trp Met Gly Phe 
        35                  40                  45              


Ser Ser Asp Tyr Lys Asp Asn His Gly Glu Tyr Pro Lys Ser Lys Asp 
    50                  55                  60                  


Ile Leu Gly Tyr Thr Asn Val His Gly Tyr Ala Tyr His Thr Ile Lys 
65                  70                  75                  80  


Thr Lys Ala Tyr Arg Leu Asn Ser Gly Asn Leu Ser Gln Thr Ile Lys 
                85                  90                  95      


Arg Ala Thr Asp Arg Phe Lys Ala Tyr Gln Lys Glu Ile Leu Arg Gly 
            100                 105                 110         


Asp Met Ser Ile Pro Ser Tyr Lys Arg Asp Ile Pro Leu Asp Leu Ile 
        115                 120                 125             


Lys Glu Asn Ile Ser Val Asn Arg Met Asn His Gly Asp Tyr Ile Ala 
    130                 135                 140                 


Ser Leu Ser Leu Leu Ser Asn Pro Ala Lys Gln Glu Met Asn Val Lys 
145                 150                 155                 160 


Arg Lys Ile Ser Val Ile Ile Ile Val Arg Gly Ala Gly Lys Thr Ile 
                165                 170                 175     


Met Asp Arg Ile Leu Ser Gly Glu Tyr Gln Val Ser Ala Ser Gln Ile 
            180                 185                 190         


Ile His Asp Asp Arg Lys Asn Lys Trp Tyr Leu Asn Ile Ser Tyr Asp 
        195                 200                 205             


Phe Glu Pro Gln Thr Arg Val Leu Asp Leu Asn Lys Ile Met Gly Ile 
    210                 215                 220                 


Ala Leu Gly Val Ala Val Ala Val Tyr Met Ala Phe Gln His Thr Pro 
225                 230                 235                 240 


Ala Arg Tyr Lys Leu Glu Gly Gly Glu Ile Glu Asn Phe Arg Arg Gln 
                245                 250                 255     


Val Glu Ser Arg Arg Ile Ser Met Leu Arg Gln Gly Lys Tyr Ala Gly 
            260                 265                 270         


Gly Ala Arg Gly Gly His Gly Arg Asp Lys Arg Ile Lys Pro Ile Glu 
        275                 280                 285             


Gln Leu Arg Asp Lys Ile Ala Asn Phe Arg Asp Thr Thr Asn His Arg 
    290                 295                 300                 


Tyr Ser Arg Tyr Ile Val Asp Met Ala Ile Lys Glu Gly Cys Gly Thr 
305                 310                 315                 320 


Ile Gln Met Glu Asp Leu Thr Asn Ile Arg Asp Ile Gly Ser Arg Phe 
                325                 330                 335     


Leu Gln Asn Trp Thr Tyr Tyr Asp Leu Gln Gln Lys Ile Ile Tyr Lys 
            340                 345                 350         


Ala Glu Glu Ala Gly Ile Lys Val Ile Lys Ile Asp Pro Gln Tyr Thr 
        355                 360                 365             


Ser Gln Arg Cys Ser Glu Cys Gly Asn Ile Asp Ser Gly Asn Arg Ile 
    370                 375                 380                 


Gly Gln Ala Ile Phe Lys Cys Arg Ala Cys Gly Tyr Glu Ala Asn Ala 
385                 390                 395                 400 


Asp Tyr Asn Ala Ala Arg Asn Ile Ala Ile Pro Asn Ile Asp Lys Ile 
                405                 410                 415     


Ile Ala Glu Ser Ile Lys Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu 
            420                 425                 430         


Ser Ala Thr Pro Glu Ser Met Ser Ser Glu Thr Gly Pro Val Ala Val 
        435                 440                 445             


Asp Pro Thr Leu Arg Arg Arg Ile Glu Pro His Glu Phe Glu Val Phe 
    450                 455                 460                 


Phe Asp Pro Arg Glu Leu Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile 
465                 470                 475                 480 


Asn Trp Gly Gly Arg His Ser Ile Trp Arg His Thr Ser Gln Asn Thr 
                485                 490                 495     


Asn Lys His Val Glu Val Asn Phe Ile Glu Lys Phe Thr Thr Glu Arg 
            500                 505                 510         


Tyr Phe Cys Pro Asn Thr Arg Cys Ser Ile Thr Trp Phe Leu Ser Trp 
        515                 520                 525             


Ser Pro Cys Gly Glu Cys Ser Arg Ala Ile Thr Glu Phe Leu Ser Arg 
    530                 535                 540                 


Tyr Pro His Val Thr Leu Phe Ile Tyr Ile Ala Arg Leu Tyr His His 
545                 550                 555                 560 


Ala Asp Pro Arg Asn Arg Gln Gly Leu Arg Asp Leu Ile Ser Ser Gly 
                565                 570                 575     


Val Thr Ile Gln Ile Met Thr Glu Gln Glu Ser Gly Tyr Cys Trp Arg 
            580                 585                 590         


Asn Phe Val Asn Tyr Ser Pro Ser Asn Glu Ala His Trp Pro Arg Tyr 
        595                 600                 605             


Pro His Leu Trp Val Arg Leu Tyr Val Leu Glu Leu Tyr Cys Ile Ile 
    610                 615                 620                 


Leu Gly Leu Pro Pro Cys Leu Asn Ile Leu Arg Arg Lys Gln Pro Gln 
625                 630                 635                 640 


Leu Thr Phe Phe Thr Ile Ala Leu Gln Ser Cys His Tyr Gln Arg Leu 
                645                 650                 655     


Pro Pro His Ile Leu Trp Ala Thr Gly Leu Lys Pro Lys Lys Lys Arg 
            660                 665                 670         


Lys Val Ser Gly Gly Ser Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu 
        675                 680                 685             


Thr Gly Lys Gln Leu Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu 
    690                 695                 700                 


Glu Val Glu Glu Val Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu Val 
705                 710                 715                 720 


His Thr Ala Tyr Asp Glu Ser Thr Asp Glu Asn Val Met Leu Leu Thr 
                725                 730                 735     


Ser Asp Ala Pro Glu Tyr Lys Pro Trp Ala Leu Val Ile Gln Asp Ser 
            740                 745                 750         


Asn Gly Glu Asn Lys Ile Lys Met Leu Ser Gly Gly Ser Pro Lys Lys 
        755                 760                 765             


Lys Arg Lys Val 
    770         


<210>  224
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C1 tile

<400>  224
catcgtcaac gtcagcatct                                                   20


<210>  225
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C2 tile

<400>  225
actcctacag ctacgacttc                                                   20


<210>  226
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C3 tile

<400>  226
atcctcaacg tcagcatcta                                                   20


<210>  227
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G1 tile

<400>  227
gtagcagttg cagtcgtaga                                                   20


<210>  228
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G2 tile

<400>  228
tgaggatgtc gatgctgaag                                                   20


<210>  229
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G3 tile

<400>  229
taggagttgc agtcgtagat                                                   20


