                         SEQUENCE LISTING

<110>  Fresenius Kabi iPSUM Srl
 
<120>  Subtilisin variants and their use

<130>  FK20233

<160>  45    

<170>  PatentIn version 3.5

<210>  1
<211>  1149
<212>  DNA
<213>  Bacillus amyloliquefaciens

<400>  1
gtgagaggca aaaaagtatg gatcagtttg ctgtttgctt tagcgttaat ctttacgatg       60

gcgttcggca gcacatcctc tgcccaggcg gcagggaaat caaacgggga aaagaaatat      120

attgtcgggt ttaaacagac aatgagcacg atgagcgccg ctaagaagaa agatgtcatt      180

tctgaaaaag gcgggaaagt gcaaaagcaa ttcaaatatg tagacgcagc ttcagctaca      240

ttaaacgaaa aagctgtaaa agaattgaaa aaagacccga gcgtcgctta cgttgaagaa      300

gatcacgtag cacatgcgta cgcgcagtcc gtgccttacg gcgtatcaca aattaaagcc      360

cctgctctgc actctcaagg ctacactgga tcaaatgtta aagtagcggt tatcgacagc      420

ggtatcgatt cttctcatcc tgatttaaag gtagcaggcg gagccagcat ggttccttct      480

gaaacaaatc ctttccaaga caacaactct cacggaactc acgttgccgg cacagttgcg      540

gctcttaata actcaatcgg tgtattaggc gttgcgccaa gcgcatcact ttacgctgta      600

aaagttctcg gtgctgacgg ttccggccaa tacagctgga tcattaacgg aatcgagtgg      660

gcgatcgcaa acaatatgga cgttattaac atgagcctcg gcggaccttc tggttctgct      720

gctttaaaag cggcagttga taaagccgtt gcatccggcg tcgtagtcgt tgcggcagcc      780

ggtaacgaag gcacttccgg cagctcaagc acagtgggct accctggtaa atacccttct      840

gtcattgcag taggcgctgt tgacagcagc aaccaaagag catctttctc aagcgtagga      900

cctgagcttg atgtcatggc acctggcgta tctatccaaa gcacgcttcc tggaaacaaa      960

tacggggcgt acaacggtac gtcaatggca tctccgcacg ttgccggagc ggctgctttg     1020

attctttcta agcacccgaa ctggacaaac actcaagtcc gcagcagttt agaaaacacc     1080

actacaaaac ttggtgattc tttctactat ggaaaagggc tgatcaacgt acaggcggca     1140

gctcagtaa                                                             1149


<210>  2
<211>  275
<212>  PRT
<213>  Bacillus amyloliquefaciens

<400>  2

Ala Gln Ser Val Pro Tyr Gly Val Ser Gln Ile Lys Ala Pro Ala Leu 
1               5                   10                  15      


His Ser Gln Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp 
            20                  25                  30          


Ser Gly Ile Asp Ser Ser His Pro Asp Leu Lys Val Ala Gly Gly Ala 
        35                  40                  45              


Ser Met Val Pro Ser Glu Thr Asn Pro Phe Gln Asp Asn Asn Ser His 
    50                  55                  60                  


Gly Thr His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly 
65                  70                  75                  80  


Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      


Gly Ala Asp Gly Ser Gly Gln Tyr Ser Trp Ile Ile Asn Gly Ile Glu 
            100                 105                 110         


Trp Ala Ile Ala Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly 
        115                 120                 125             


Pro Ser Gly Ser Ala Ala Leu Lys Ala Ala Val Asp Lys Ala Val Ala 
    130                 135                 140                 


Ser Gly Val Val Val Val Ala Ala Ala Gly Asn Glu Gly Thr Ser Gly 
145                 150                 155                 160 


Ser Ser Ser Thr Val Gly Tyr Pro Gly Lys Tyr Pro Ser Val Ile Ala 
                165                 170                 175     


Val Gly Ala Val Asp Ser Ser Asn Gln Arg Ala Ser Phe Ser Ser Val 
            180                 185                 190         


Gly Pro Glu Leu Asp Val Met Ala Pro Gly Val Ser Ile Gln Ser Thr 
        195                 200                 205             


Leu Pro Gly Asn Lys Tyr Gly Ala Tyr Asn Gly Thr Ser Met Ala Ser 
    210                 215                 220                 


Pro His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn 
225                 230                 235                 240 


Trp Thr Asn Thr Gln Val Arg Ser Ser Leu Glu Asn Thr Thr Thr Lys 
                245                 250                 255     


Leu Gly Asp Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         


Ala Ala Gln 
        275 


<210>  3
<211>  272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  subtilisin BPN' variant of the invention


<220>
<221>  VARIANT
<222>  (87)..(87)
<223>  L96 position

<220>
<221>  VARIANT
<222>  (90)..(90)
<223>  D99 position

<220>
<221>  VARIANT
<222>  (214)..(214)
<223>  A223 position

<220>
<221>  VARIANT
<222>  (215)..(215)
<223>  S224 position

<400>  3

Ala Gln Ser Val Pro Tyr Gly Val Ser Gln Ile Lys Ala Pro Ala Leu 
1               5                   10                  15      


His Ser Gln Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp 
            20                  25                  30          


Ser Gly Ile Asp Ser Ser His Pro Asp Leu Lys Val Ala Gly Gly Ala 
        35                  40                  45              


Ser Met Val Pro Ser Glu Thr Asn Pro Phe Gln Asp Asn Asn Ser His 
    50                  55                  60                  


Gly Thr His Val Ala Gly Thr Val Ala Ala Val Ala Pro Ser Ala Ser 
65                  70                  75                  80  


Leu Tyr Ala Val Lys Val Xaa Gly Ala Xaa Gly Ser Gly Gln Tyr Ser 
                85                  90                  95      


Trp Ile Ile Asn Gly Ile Glu Trp Ala Ile Ala Asn Asn Met Asp Val 
            100                 105                 110         


Ile Asn Met Ser Leu Gly Gly Pro Ser Gly Ser Ala Ala Leu Lys Ala 
        115                 120                 125             


Ala Val Asp Lys Ala Val Ala Ser Gly Val Val Val Val Ala Ala Ala 
    130                 135                 140                 


Gly Asn Glu Gly Thr Ser Gly Ser Ser Ser Thr Val Gly Tyr Pro Gly 
145                 150                 155                 160 


Lys Tyr Pro Ser Val Ile Ala Val Gly Ala Val Asp Ser Ser Asn Gln 
                165                 170                 175     


Arg Ala Ser Phe Ser Ser Val Gly Pro Glu Leu Asp Val Met Ala Pro 
            180                 185                 190         


Gly Val Ser Ile Gln Ser Thr Leu Pro Gly Asn Lys Tyr Gly Ala Tyr 
        195                 200                 205             


Asn Gly Thr Cys Met Xaa Xaa Pro His Val Ala Gly Ala Ala Ala Leu 
    210                 215                 220                 


Ile Leu Ser Lys His Pro Asn Trp Thr Asn Thr Gln Val Arg Ser Ser 
225                 230                 235                 240 


Leu Glu Asn Thr Thr Thr Lys Leu Gly Asp Ser Phe Tyr Tyr Gly Lys 
                245                 250                 255     


Gly Leu Ile Asn Val Gln Ala Ala Ala Gln His His His His His His 
            260                 265                 270         


<210>  4
<211>  272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Reference enzyme Ptl-84

<400>  4

Ala Lys Cys Val Ser Tyr Gly Val Ala Gln Ile Lys Ala Pro Ala Leu 
1               5                   10                  15      


His Ser Gln Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Ser Gly Ile Asp Ser Ser His Pro Asp Leu Asn Val Ala Gly Gly Ala 
        35                  40                  45              


Ser Phe Val Pro Ser Glu Thr Asn Pro Phe Gln Asp Asn Ala Ser His 
    50                  55                  60                  


Gly Thr His Val Ala Gly Thr Val Leu Ala Val Ala Pro Ser Ala Ser 
65                  70                  75                  80  


Leu Tyr Ala Val Lys Val Leu Gly Ala Asp Gly Ser Gly Gln Tyr Ser 
                85                  90                  95      


Trp Ile Ile Asn Gly Ile Glu Trp Ala Ile Ala Asn Asn Met Asp Val 
            100                 105                 110         


Ile Asn Met Ser Leu Gly Gly Pro Ser Gly Ser Ala Ala Leu Lys Ala 
        115                 120                 125             


Ala Val Asp Lys Ala Val Ala Ser Gly Val Val Val Val Ala Ala Ala 
    130                 135                 140                 


Gly Asn Asn Gly Thr Ser Gly Ser Ser Ser Thr Val Glu Tyr Pro Ala 
145                 150                 155                 160 


Lys Tyr Pro Ser Val Ile Ala Val Gly Ala Val Asp Ser Ser Asn Gln 
                165                 170                 175     


Arg Ala Pro Trp Ser Ser Val Gly Pro Glu Leu Asp Val Met Ala Pro 
            180                 185                 190         


Gly Val Ser Ile Cys Ser Thr Leu Pro Gly Gly Lys Tyr Gly Ala His 
        195                 200                 205             


Asp Gly Thr Cys Pro Ala Ser Asn His Val Ala Gly Ala Ala Ala Leu 
    210                 215                 220                 


Ile Leu Ser Lys His Pro Asn Trp Thr Asn Thr Gln Val Arg Ser Ser 
225                 230                 235                 240 


Leu Glu Asn Thr Ala Thr Lys Leu Gly Asp Ser Phe Tyr Tyr Gly Lys 
                245                 250                 255     


Gly Leu Ile Asn Val Glu Ala Ala Ala Gln His His His His His His 
            260                 265                 270         


<210>  5
<211>  272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Reference enzyme Ptl-79

<400>  5

Ala Lys Cys Val Ser Tyr Gly Val Ala Gln Ile Lys Ala Pro Ala Leu 
1               5                   10                  15      


His Ser Gln Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Ser Gly Ile Asp Ser Ser His Pro Asp Leu Asn Val Ala Gly Gly Ala 
        35                  40                  45              


Ser Phe Val Pro Ser Glu Thr Asn Pro Phe Gln Asp Asn Asn Ser His 
    50                  55                  60                  


Gly Thr His Val Ala Gly Thr Val Leu Ala Val Ala Pro Ser Ala Ser 
65                  70                  75                  80  


Leu Tyr Ala Val Lys Val Leu Gly Ala Asp Gly Ser Gly Gln Tyr Ser 
                85                  90                  95      


Trp Val Ile Asn Gly Ile Glu Trp Ala Ile Ala Asn Asn Met Asp Val 
            100                 105                 110         


Ile Asn Met Ser Leu Gly Gly Pro Ser Gly Ser Ala Ala Leu Lys Ala 
        115                 120                 125             


Ala Val Asp Lys Ala Val Ala Ser Gly Val Val Val Val Ala Ala Ala 
    130                 135                 140                 


Gly Asn Ser Gly Thr Ser Gly Ser Ser Ser Thr Val Ser Tyr Pro Ala 
145                 150                 155                 160 


Lys Tyr Pro Ser Val Ile Ala Val Gly Ala Val Asp Ser Ser Asn Gln 
                165                 170                 175     


Arg Ala Pro Trp Ser Ser Val Gly Pro Glu Leu Asp Val Met Ala Pro 
            180                 185                 190         


Gly Val Ser Ile Cys Ser Thr Leu Pro Gly Gly Lys Tyr Gly Ala His 
        195                 200                 205             


Ser Gly Thr Cys Pro Ala Ser Asn His Val Ala Gly Ala Ala Ala Leu 
    210                 215                 220                 


Ile Leu Ser Lys His Pro Asn Trp Thr Asn Thr Gln Val Arg Ser Ser 
225                 230                 235                 240 


Leu Glu Asn Thr Ala Thr Lys Leu Gly Asp Ser Phe Tyr Tyr Gly Lys 
                245                 250                 255     


Gly Leu Ile Asn Val Glu Ala Ala Ala Gln His His His His His His 
            260                 265                 270         


<210>  6
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  liraglutide


<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  Lys is Lys(Pal-gamma-Glu)

<400>  6

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  7
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  semaglutide


<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Aib

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  Lys is Lys(AEEA-AEEA-gamma-Glu-17-carboxyheptadecanoyl)

<400>  7

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  8
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  dasiglucagon


<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Aib

<400>  8

His Ser Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Xaa 
1               5                   10                  15      


Ala Arg Ala Glu Glu Phe Val Lys Trp Leu Glu Ser Thr 
            20                  25                  


<210>  9
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glepaglutide


<220>
<221>  MOD_RES
<222>  (39)..(39)
<223>  AMIDATION C-terminal

<400>  9

His Gly Glu Gly Thr Phe Ser Ser Glu Leu Ala Thr Ile Leu Asp Ala 
1               5                   10                  15      


Leu Ala Ala Arg Asp Phe Ile Ala Trp Leu Ile Ala Thr Lys Ile Thr 
            20                  25                  30          


Asp Lys Lys Lys Lys Lys Lys 
        35                  


<210>  10
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  elsiglutide


<220>
<221>  MOD_RES
<222>  (39)..(39)
<223>  AMIDATION C-terminal

<400>  10

His Gly Glu Gly Ser Phe Ser Ser Glu Leu Ser Thr Ile Leu Asp Ala 
1               5                   10                  15      


Leu Ala Ala Arg Asp Phe Ile Ala Trp Leu Ile Ala Thr Lys Ile Thr 
            20                  25                  30          


Asp Lys Lys Lys Lys Lys Lys 
        35                  


<210>  11
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  teriparatide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal phenylacetylation might be present in the teriparatide
       precursor

<400>  11

Ser Val Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His Leu Asn 
1               5                   10                  15      


Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp Val His 
            20                  25                  30          


Asn Phe 
        


<210>  12
<211>  32
<212>  PRT
<213>  Salmo salar


<220>
<221>  DISULFID
<222>  (1)..(7)

<220>
<221>  MOD_RES
<222>  (32)..(32)
<223>  AMIDATION C-terminal

<400>  12

Cys Ser Asn Leu Ser Thr Cys Val Leu Gly Lys Leu Ser Gln Glu Leu 
1               5                   10                  15      


His Lys Leu Gln Thr Tyr Pro Arg Thr Asn Thr Gly Ser Gly Thr Pro 
            20                  25                  30          


<210>  13
<211>  20
<212>  PRT
<213>  artificial sequence

<220>
<223>  bivalirudin


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  D-Phe

<400>  13

Xaa Pro Arg Pro Gly Gly Gly Gly Asn Gly Asp Phe Glu Glu Ile Pro 
1               5                   10                  15      


Glu Glu Tyr Leu 
            20  


<210>  14
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  8-mer model peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  ACETYLATION N-terminal

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  AMIDATION C-terminal

<400>  14

Asp Phe Ser Lys Leu Ala Leu Arg 
1               5               


<210>  15
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Dasi 1-8


<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  C-terminal (thio)ester

<400>  15

His Ser Gln Gly Thr Phe Thr Ser 
1               5               


<210>  16
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Dasi 9-29


<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Aib

<400>  16

Asp Tyr Ser Lys Tyr Leu Asp Xaa Ala Arg Ala Glu Glu Phe Val Lys 
1               5                   10                  15      


Trp Leu Glu Ser Thr 
            20      


<210>  17
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Dasi 1-9


<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  C-terminal (thio)ester

<400>  17

His Ser Gln Gly Thr Phe Thr Ser Asp 
1               5                   


<210>  18
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Dasi 10-29


<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Aib

<400>  18

Tyr Ser Lys Tyr Leu Asp Xaa Ala Arg Ala Glu Glu Phe Val Lys Trp 
1               5                   10                  15      


Leu Glu Ser Thr 
            20  


<210>  19
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Dasi 1-10


<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  C-terminal (thio)ester

<400>  19

His Ser Gln Gly Thr Phe Thr Ser Asp Tyr 
1               5                   10  


<210>  20
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Dasi 11-29


<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  Aib

<400>  20

Ser Lys Tyr Leu Asp Xaa Ala Arg Ala Glu Glu Phe Val Lys Trp Leu 
1               5                   10                  15      


Glu Ser Thr 
            


<210>  21
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glepa 1-11


<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  C-terminal (thio)ester

<400>  21

His Gly Glu Gly Thr Phe Ser Ser Glu Leu Ala 
1               5                   10      


<210>  22
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glepa 12-39

<400>  22

Thr Ile Leu Asp Ala Leu Ala Ala Arg Asp Phe Ile Ala Trp Leu Ile 
1               5                   10                  15      


Ala Thr Lys Ile Thr Asp Lys Lys Lys Lys Lys Lys 
            20                  25              


<210>  23
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glepa 1-16


<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  C-terminal (thio)ester

<400>  23

His Gly Glu Gly Thr Phe Ser Ser Glu Leu Ala Thr Ile Leu Asp Ala 
1               5                   10                  15      


<210>  24
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glepa 17-39

<400>  24

Leu Ala Ala Arg Asp Phe Ile Ala Trp Leu Ile Ala Thr Lys Ile Thr 
1               5                   10                  15      


Asp Lys Lys Lys Lys Lys Lys 
            20              


<210>  25
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Elsi 1-11


<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  C-terminal (thio)ester

<400>  25

His Gly Glu Gly Ser Phe Ser Ser Glu Leu Ser 
1               5                   10      


<210>  26
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Elsi 12-39

<400>  26

Thr Ile Leu Asp Ala Leu Ala Ala Arg Asp Phe Ile Ala Trp Leu Ile 
1               5                   10                  15      


Ala Thr Lys Ile Thr Asp Lys Lys Lys Lys Lys Lys 
            20                  25              


<210>  27
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Elsi 1-16


<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  C-terminal (thio)ester

<400>  27

His Gly Glu Gly Ser Phe Ser Ser Glu Leu Ser Thr Ile Leu Asp Ala 
1               5                   10                  15      


<210>  28
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Elsi 17-39

<400>  28

Leu Ala Ala Arg Asp Phe Ile Ala Trp Leu Ile Ala Thr Lys Ile Thr 
1               5                   10                  15      


Asp Lys Lys Lys Lys Lys Lys 
            20              


<210>  29
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Teri 1-11


<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  C-terminal (thio)ester

<400>  29

Ser Val Ser Glu Ile Gln Leu Met His Asn Leu 
1               5                   10      


<210>  30
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Teri 12-34

<400>  30

Gly Lys His Leu Asn Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys 
1               5                   10                  15      


Leu Gln Asp Val His Asn Phe 
            20              


<210>  31
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Calci 1-12


<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  C-terminal (thio)ester

<400>  31

Cys Ser Asn Leu Ser Thr Cys Val Leu Gly Lys Leu 
1               5                   10          


<210>  32
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Calci 13-32


<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  AMIDATION C-terminal might be present

<400>  32

Ser Gln Glu Leu His Lys Leu Gln Thr Tyr Pro Arg Thr Asn Thr Gly 
1               5                   10                  15      


Ser Gly Thr Pro 
            20  


<210>  33
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Calci 1-20


<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  C-terminal (thio)ester

<400>  33

Cys Ser Asn Leu Ser Thr Cys Val Leu Gly Lys Leu Ser Gln Glu Leu 
1               5                   10                  15      


His Lys Leu Gln 
            20  


<210>  34
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Calci 21-32

<400>  34

Thr Tyr Pro Arg Thr Asn Thr Gly Ser Gly Thr Pro 
1               5                   10          


<210>  35
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biva 1-10


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  D-Phe

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  C-terminal (thio)ester

<400>  35

Xaa Pro Arg Pro Gly Gly Gly Gly Asn Gly 
1               5                   10  


<210>  36
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biva 11-20

<400>  36

Asp Phe Glu Glu Ile Pro Glu Glu Tyr Leu 
1               5                   10  


<210>  37
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lira-Sema 1-11


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Ala or Aib

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  C-terminal (thio)ester

<400>  37

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser 
1               5                   10      


<210>  38
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lira-Sema 12-31


<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Lys is selected from Lys, Lys(PG), Lys(gamma-Glu), 
       Lys(Pal-gamma-Glu) and 
       Lys(AEEA-AEEA-gamma-Glu-17-carboxyheptadecanoyl)

<400>  38

Ser Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val 
1               5                   10                  15      


Arg Gly Arg Gly 
            20  


<210>  39
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lira 1-11


<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  C-terminal  -OCam-Phe-Lys-NH2 ester

<400>  39

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser 
1               5                   10      


<210>  40
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lira 12-31


<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Lys is Lys(Pal-gamma-Glu)

<400>  40

Ser Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val 
1               5                   10                  15      


Arg Gly Arg Gly 
            20  


<210>  41
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  model peptide fragment


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  ACETYLATION N-terminal

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  C-terminal  -OCam-Leu-OH ester or OH

<400>  41

Asp Phe Ser Lys Leu 
1               5   


<210>  42
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  lira 1-11-1-31


<220>
<221>  MOD_RES
<222>  (31)..(31)
<223>  Lys is Lys(Pal-gamma-Glu)

<400>  42

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser His Ala Glu Gly Thr 
1               5                   10                  15      


Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala Ala Lys Glu 
            20                  25                  30          


Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
        35                  40          


<210>  43
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Teri 1-11 ester


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal phenylacetilation

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  C-terminal -OCam-Leu-OH ester

<400>  43

Ser Val Ser Glu Ile Gln Leu Met His Asn Leu 
1               5                   10      


<210>  44
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sema 1-11


<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Aib

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  C-terminal -OCam-Phe-Lys-NH2 ester

<400>  44

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser 
1               5                   10      


<210>  45
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sema 12-31


<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Lys is Lys(AEEA-AEEA-gamma-Glu-17-carboxyheptadecanoyl)

<400>  45

Ser Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val 
1               5                   10                  15      


Arg Gly Arg Gly 
            20  


