               SEQUENCE LISTING

<110> LivingMed Biotech S.R.L.

<120> GENETICALLY MODIFIED CLOSTRIDIUM STRAINS EXPRESSING RECOMBINANT ANTIGENS AND USES THEREOF

<130> 307-001P

<150> EP21157234.2
<151> 2021-02-15

<160> 32

<170> BiSSAP 1.3.6

<210> 1
<211> 39
<212> DNA
<213> Artificial Sequence


<220> 
<223> Pptb-BsaI-F

<400> 1
tacgggtctc gtccataagt cagcagaaag tataatgag                            39


<210> 2
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> RBD-BsaI-R

<400> 2
tataggtctc tgtctttaaa agtttacgca tttgttcttc actagattag                50


<210> 3
<211> 44
<212> DNA
<213> Artificial Sequence


<220> 
<223> Lig-BsaI-R

<400> 3
tataggtctc tgtctttatg gacgttgtgg agttgaatga tatg                      44


<210> 4
<211> 42
<212> DNA
<213> Artificial Sequence


<220> 
<223> Pfdx-BsaI-F

<400> 4
tacgggtctc gtccagtgta gtagcctgcg aaataagtaa gg                        42


<210> 5
<211> 41
<212> DNA
<213> Artificial Sequence


<220> 
<223> NC-BsaI-R

<400> 5
tataggtctc tgtctttagg cctgagttga gtcagcactg c                         41


<210> 6
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> pGGv2-R

<400> 6
ctgttgaacc attagccgtc tc                                             22


<210> 7
<211> 17
<212> DNA
<213> Artificial Sequence


<220> 
<223> pGGv2-F

<400> 7
ccgatcgcgt ctcctgg                                                   17


<210> 8
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> M13F

<400> 8
gtaaaacgac ggccag                                                    16


<210> 9
<211> 17
<212> DNA
<213> Artificial Sequence


<220> 
<223> M13R

<400> 9
caggaaacag ctatgac                                                   17


<210> 10
<211> 420
<212> PRT
<213> Artificial Sequence


<220> 
<223> Spike protein (aac 251-670)

<400> 10
Pro Gly Asp Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr 
1               5                   10                  15      
Val Gly Tyr Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn 
            20                  25                  30          
Gly Thr Ile Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu 
        35                  40                  45              
Thr Lys Cys Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln 
    50                  55                  60                  
Thr Ser Asn Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro 
65                  70                  75                  80  
Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
                85                  90                  95      
Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            100                 105                 110         
Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        115                 120                 125             
Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    130                 135                 140                 
Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
145                 150                 155                 160 
Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                165                 170                 175     
Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            180                 185                 190         
Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        195                 200                 205             
Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    210                 215                 220                 
Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
225                 230                 235                 240 
Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                245                 250                 255     
Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            260                 265                 270         
Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
        275                 280                 285             
Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu 
    290                 295                 300                 
Ser Asn Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala 
305                 310                 315                 320 
Asp Thr Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp 
                325                 330                 335     
Ile Thr Pro Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr 
            340                 345                 350         
Asn Thr Ser Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr 
        355                 360                 365             
Glu Val Pro Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg 
    370                 375                 380                 
Val Tyr Ser Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu 
385                 390                 395                 400 
Ile Gly Ala Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile 
                405                 410                 415     
Gly Ala Gly Ile 
            420 

<210> 11
<211> 223
<212> PRT
<213> Artificial Sequence


<220> 
<223> Spike protein (Full RBD, RBD1)

<400> 11
Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      
Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          
Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              
Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  
Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  
Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      
Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         
Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             
Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 
Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 
Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     
Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         
Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             
Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             

<210> 12
<211> 257
<212> PRT
<213> Artificial Sequence


<220> 
<223> Spike protein (Full RBD, RBD1-L)

<400> 12
Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      
Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          
Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              
Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  
Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  
Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      
Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         
Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             
Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 
Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 
Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     
Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         
Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             
Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Gly 
    210                 215                 220                 
Gly Gly Gly Ser Ser Phe His Gln Leu Pro Ala Arg Ser Pro Leu Pro 
225                 230                 235                 240 
Gly Gly Gly Gly Ser Phe Tyr Pro Ser Tyr His Ser Thr Pro Gln Arg 
                245                 250                 255     
Pro 
    

<210> 13
<211> 211
<212> PRT
<213> Artificial Sequence


<220> 
<223> Spike protein (RBD0)

<400> 13
Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      
Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          
Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              
Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  
Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  
Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      
Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         
Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             
Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 
Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 
Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     
Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         
Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
        195                 200                 205             
Val Asn Phe 
    210     

<210> 14
<211> 245
<212> PRT
<213> Artificial Sequence


<220> 
<223> Spike protein (RBD0-L fusion protein)

<400> 14
Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      
Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          
Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              
Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  
Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  
Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      
Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         
Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             
Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 
Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 
Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     
Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         
Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
        195                 200                 205             
Val Asn Phe Gly Gly Gly Gly Ser Ser Phe His Gln Leu Pro Ala Arg 
    210                 215                 220                 
Ser Pro Leu Pro Gly Gly Gly Gly Ser Phe Tyr Pro Ser Tyr His Ser 
225                 230                 235                 240 
Thr Pro Gln Arg Pro 
                245 

<210> 15
<211> 263
<212> PRT
<213> Artificial Sequence


<220> 
<223> Spike protein (RBD2)

<400> 15
Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu 
1               5                   10                  15      
Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg 
            20                  25                  30          
Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu 
        35                  40                  45              
Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr 
    50                  55                  60                  
Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val 
65                  70                  75                  80  
Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
                85                  90                  95      
Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser 
            100                 105                 110         
Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr 
        115                 120                 125             
Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly 
    130                 135                 140                 
Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly 
145                 150                 155                 160 
Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro 
                165                 170                 175     
Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro 
            180                 185                 190         
Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr 
        195                 200                 205             
Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val 
    210                 215                 220                 
Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro 
225                 230                 235                 240 
Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe 
                245                 250                 255     
Asn Gly Leu Thr Gly Thr Gly 
            260             

<210> 16
<211> 297
<212> PRT
<213> Artificial Sequence


<220> 
<223> Spike protein (RBD2-L fusion protein)

<400> 16
Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu 
1               5                   10                  15      
Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg 
            20                  25                  30          
Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu 
        35                  40                  45              
Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr 
    50                  55                  60                  
Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val 
65                  70                  75                  80  
Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
                85                  90                  95      
Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser 
            100                 105                 110         
Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr 
        115                 120                 125             
Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly 
    130                 135                 140                 
Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly 
145                 150                 155                 160 
Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro 
                165                 170                 175     
Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro 
            180                 185                 190         
Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr 
        195                 200                 205             
Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val 
    210                 215                 220                 
Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro 
225                 230                 235                 240 
Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe 
                245                 250                 255     
Asn Gly Leu Thr Gly Thr Gly Gly Gly Gly Gly Ser Ser Phe His Gln 
            260                 265                 270         
Leu Pro Ala Arg Ser Pro Leu Pro Gly Gly Gly Gly Ser Phe Tyr Pro 
        275                 280                 285             
Ser Tyr His Ser Thr Pro Gln Arg Pro 
    290                 295         

<210> 17
<211> 418
<212> PRT
<213> Artificial Sequence


<220> 
<223> NC protein

<400> 17
Ser Asp Asn Gly Pro Gln Asn Gln Arg Asn Ala Pro Arg Ile Thr Phe 
1               5                   10                  15      
Gly Gly Pro Ser Asp Ser Thr Gly Ser Asn Gln Asn Gly Glu Arg Ser 
            20                  25                  30          
Gly Ala Arg Ser Lys Gln Arg Arg Pro Gln Gly Leu Pro Asn Asn Thr 
        35                  40                  45              
Ala Ser Trp Phe Thr Ala Leu Thr Gln His Gly Lys Glu Asp Leu Lys 
    50                  55                  60                  
Phe Pro Arg Gly Gln Gly Val Pro Ile Asn Thr Asn Ser Ser Pro Asp 
65                  70                  75                  80  
Asp Gln Ile Gly Tyr Tyr Arg Arg Ala Thr Arg Arg Ile Arg Gly Gly 
                85                  90                  95      
Asp Gly Lys Met Lys Asp Leu Ser Pro Arg Trp Tyr Phe Tyr Tyr Leu 
            100                 105                 110         
Gly Thr Gly Pro Glu Ala Gly Leu Pro Tyr Gly Ala Asn Lys Asp Gly 
        115                 120                 125             
Ile Ile Trp Val Ala Thr Glu Gly Ala Leu Asn Thr Pro Lys Asp His 
    130                 135                 140                 
Ile Gly Thr Arg Asn Pro Ala Asn Asn Ala Ala Ile Val Leu Gln Leu 
145                 150                 155                 160 
Pro Gln Gly Thr Thr Leu Pro Lys Gly Phe Tyr Ala Glu Gly Ser Arg 
                165                 170                 175     
Gly Gly Ser Gln Ala Ser Ser Arg Ser Ser Ser Arg Ser Arg Asn Ser 
            180                 185                 190         
Ser Arg Asn Ser Thr Pro Gly Ser Ser Arg Gly Thr Ser Pro Ala Arg 
        195                 200                 205             
Met Ala Gly Asn Gly Gly Asp Ala Ala Leu Ala Leu Leu Leu Leu Asp 
    210                 215                 220                 
Arg Leu Asn Gln Leu Glu Ser Lys Met Ser Gly Lys Gly Gln Gln Gln 
225                 230                 235                 240 
Gln Gly Gln Thr Val Thr Lys Lys Ser Ala Ala Glu Ala Ser Lys Lys 
                245                 250                 255     
Pro Arg Gln Lys Arg Thr Ala Thr Lys Ala Tyr Asn Val Thr Gln Ala 
            260                 265                 270         
Phe Gly Arg Arg Gly Pro Glu Gln Thr Gln Gly Asn Phe Gly Asp Gln 
        275                 280                 285             
Glu Leu Ile Arg Gln Gly Thr Asp Tyr Lys His Trp Pro Gln Ile Ala 
    290                 295                 300                 
Gln Phe Ala Pro Ser Ala Ser Ala Phe Phe Gly Met Ser Arg Ile Gly 
305                 310                 315                 320 
Met Glu Val Thr Pro Ser Gly Thr Trp Leu Thr Tyr Thr Gly Ala Ile 
                325                 330                 335     
Lys Leu Asp Asp Lys Asp Pro Asn Phe Lys Asp Gln Val Ile Leu Leu 
            340                 345                 350         
Asn Lys His Ile Asp Ala Tyr Lys Thr Phe Pro Pro Thr Glu Pro Lys 
        355                 360                 365             
Lys Asp Lys Lys Lys Lys Ala Asp Glu Thr Gln Ala Leu Pro Gln Arg 
    370                 375                 380                 
Gln Lys Lys Gln Gln Thr Val Thr Leu Leu Pro Ala Ala Asp Leu Asp 
385                 390                 395                 400 
Asp Phe Ser Lys Gln Leu Gln Gln Ser Met Ser Ser Ala Asp Ser Thr 
                405                 410                 415     
Gln Ala 
        

<210> 18
<211> 453
<212> PRT
<213> Artificial Sequence


<220> 
<223> NC protein, NC-L fusion protein

<400> 18
Ser Asp Asn Gly Pro Gln Asn Gln Arg Asn Ala Pro Arg Ile Thr Phe 
1               5                   10                  15      
Gly Gly Pro Ser Asp Ser Thr Gly Ser Asn Gln Asn Gly Glu Arg Ser 
            20                  25                  30          
Gly Ala Arg Ser Lys Gln Arg Arg Pro Gln Gly Leu Pro Asn Asn Thr 
        35                  40                  45              
Ala Ser Trp Phe Thr Ala Leu Thr Gln His Gly Lys Glu Asp Leu Lys 
    50                  55                  60                  
Phe Pro Arg Gly Gln Gly Val Pro Ile Asn Thr Asn Ser Ser Pro Asp 
65                  70                  75                  80  
Asp Gln Ile Gly Tyr Tyr Arg Arg Ala Thr Arg Arg Ile Arg Gly Gly 
                85                  90                  95      
Asp Gly Lys Met Lys Asp Leu Ser Pro Arg Trp Tyr Phe Tyr Tyr Leu 
            100                 105                 110         
Gly Thr Gly Pro Glu Ala Gly Leu Pro Tyr Gly Ala Asn Lys Asp Gly 
        115                 120                 125             
Ile Ile Trp Val Ala Thr Glu Gly Ala Leu Asn Thr Pro Lys Asp His 
    130                 135                 140                 
Ile Gly Thr Arg Asn Pro Ala Asn Asn Ala Ala Ile Val Leu Gln Leu 
145                 150                 155                 160 
Pro Gln Gly Thr Thr Leu Pro Lys Gly Phe Tyr Ala Glu Gly Ser Arg 
                165                 170                 175     
Gly Gly Ser Gln Ala Ser Ser Arg Ser Ser Ser Arg Ser Arg Asn Ser 
            180                 185                 190         
Ser Arg Asn Ser Thr Pro Gly Ser Ser Arg Gly Thr Ser Pro Ala Arg 
        195                 200                 205             
Met Ala Gly Asn Gly Gly Asp Ala Ala Leu Ala Leu Leu Leu Leu Asp 
    210                 215                 220                 
Arg Leu Asn Gln Leu Glu Ser Lys Met Ser Gly Lys Gly Gln Gln Gln 
225                 230                 235                 240 
Gln Gly Gln Thr Val Thr Lys Lys Ser Ala Ala Glu Ala Ser Lys Lys 
                245                 250                 255     
Pro Arg Gln Lys Arg Thr Ala Thr Lys Ala Tyr Asn Val Thr Gln Ala 
            260                 265                 270         
Phe Gly Arg Arg Gly Pro Glu Gln Thr Gln Gly Asn Phe Gly Asp Gln 
        275                 280                 285             
Glu Leu Ile Arg Gln Gly Thr Asp Tyr Lys His Trp Pro Gln Ile Ala 
    290                 295                 300                 
Gln Phe Ala Pro Ser Ala Ser Ala Phe Phe Gly Met Ser Arg Ile Gly 
305                 310                 315                 320 
Met Glu Val Thr Pro Ser Gly Thr Trp Leu Thr Tyr Thr Gly Ala Ile 
                325                 330                 335     
Lys Leu Asp Asp Lys Asp Pro Asn Phe Lys Asp Gln Val Ile Leu Leu 
            340                 345                 350         
Asn Lys His Ile Asp Ala Tyr Lys Thr Phe Pro Pro Thr Glu Pro Lys 
        355                 360                 365             
Lys Asp Lys Lys Lys Lys Ala Asp Glu Thr Gln Ala Leu Pro Gln Arg 
    370                 375                 380                 
Gln Lys Lys Gln Gln Thr Val Thr Leu Leu Pro Ala Ala Asp Leu Asp 
385                 390                 395                 400 
Asp Phe Ser Lys Gln Leu Gln Gln Ser Met Ser Ser Ala Asp Ser Thr 
                405                 410                 415     
Gln Ala Gly Gly Gly Gly Gly Ser Ser Phe His Gln Leu Pro Ala Arg 
            420                 425                 430         
Ser Pro Leu Pro Gly Gly Gly Gly Ser Phe Tyr Pro Ser Tyr His Ser 
        435                 440                 445             
Thr Pro Gln Arg Pro 
    450             

<210> 19
<211> 120
<212> PRT
<213> Artificial Sequence


<220> 
<223> NC protein (LKR region)

<400> 19
Phe Tyr Ala Glu Gly Ser Arg Gly Gly Ser Gln Ala Ser Ser Arg Ser 
1               5                   10                  15      
Ser Ser Arg Ser Arg Asn Ser Ser Arg Asn Ser Thr Pro Gly Ser Ser 
            20                  25                  30          
Arg Gly Thr Ser Pro Ala Arg Met Ala Gly Asn Gly Gly Asp Ala Ala 
        35                  40                  45              
Leu Ala Leu Leu Leu Leu Asp Arg Leu Asn Gln Leu Glu Ser Lys Met 
    50                  55                  60                  
Ser Gly Lys Gly Gln Gln Gln Gln Gly Gln Thr Val Thr Lys Lys Ser 
65                  70                  75                  80  
Ala Ala Glu Ala Ser Lys Lys Pro Arg Gln Lys Arg Thr Ala Thr Lys 
                85                  90                  95      
Ala Tyr Asn Val Thr Gln Ala Phe Gly Arg Arg Gly Pro Glu Gln Thr 
            100                 105                 110         
Gln Gly Asn Phe Gly Asp Gln Glu 
        115                 120 

<210> 20
<211> 155
<212> PRT
<213> Artificial Sequence


<220> 
<223> NC protein (LKR-L fusion protein)

<400> 20
Phe Tyr Ala Glu Gly Ser Arg Gly Gly Ser Gln Ala Ser Ser Arg Ser 
1               5                   10                  15      
Ser Ser Arg Ser Arg Asn Ser Ser Arg Asn Ser Thr Pro Gly Ser Ser 
            20                  25                  30          
Arg Gly Thr Ser Pro Ala Arg Met Ala Gly Asn Gly Gly Asp Ala Ala 
        35                  40                  45              
Leu Ala Leu Leu Leu Leu Asp Arg Leu Asn Gln Leu Glu Ser Lys Met 
    50                  55                  60                  
Ser Gly Lys Gly Gln Gln Gln Gln Gly Gln Thr Val Thr Lys Lys Ser 
65                  70                  75                  80  
Ala Ala Glu Ala Ser Lys Lys Pro Arg Gln Lys Arg Thr Ala Thr Lys 
                85                  90                  95      
Ala Tyr Asn Val Thr Gln Ala Phe Gly Arg Arg Gly Pro Glu Gln Thr 
            100                 105                 110         
Gln Gly Asn Phe Gly Asp Gln Glu Gly Gly Gly Gly Gly Ser Ser Phe 
        115                 120                 125             
His Gln Leu Pro Ala Arg Ser Pro Leu Pro Gly Gly Gly Gly Ser Phe 
    130                 135                 140                 
Tyr Pro Ser Tyr His Ser Thr Pro Gln Arg Pro 
145                 150                 155 

<210> 21
<211> 306
<212> PRT
<213> Artificial Sequence


<220> 
<223> Mpro protein

<400> 21
Ser Gly Phe Arg Lys Met Ala Phe Pro Ser Gly Lys Val Glu Gly Cys 
1               5                   10                  15      
Met Val Gln Val Thr Cys Gly Thr Thr Thr Leu Asn Gly Leu Trp Leu 
            20                  25                  30          
Asp Asp Val Val Tyr Cys Pro Arg His Val Ile Cys Thr Ser Glu Asp 
        35                  40                  45              
Met Leu Asn Pro Asn Tyr Glu Asp Leu Leu Ile Arg Lys Ser Asn His 
    50                  55                  60                  
Asn Phe Leu Val Gln Ala Gly Asn Val Gln Leu Arg Val Ile Gly His 
65                  70                  75                  80  
Ser Met Gln Asn Cys Val Leu Lys Leu Lys Val Asp Thr Ala Asn Pro 
                85                  90                  95      
Lys Thr Pro Lys Tyr Lys Phe Val Arg Ile Gln Pro Gly Gln Thr Phe 
            100                 105                 110         
Ser Val Leu Ala Cys Tyr Asn Gly Ser Pro Ser Gly Val Tyr Gln Cys 
        115                 120                 125             
Ala Met Arg Pro Asn Phe Thr Ile Lys Gly Ser Phe Leu Asn Gly Ser 
    130                 135                 140                 
Cys Gly Ser Val Gly Phe Asn Ile Asp Tyr Asp Cys Val Ser Phe Cys 
145                 150                 155                 160 
Tyr Met His His Met Glu Leu Pro Thr Gly Val His Ala Gly Thr Asp 
                165                 170                 175     
Leu Glu Gly Asn Phe Tyr Gly Pro Phe Val Asp Arg Gln Thr Ala Gln 
            180                 185                 190         
Ala Ala Gly Thr Asp Thr Thr Ile Thr Val Asn Val Leu Ala Trp Leu 
        195                 200                 205             
Tyr Ala Ala Val Ile Asn Gly Asp Arg Trp Phe Leu Asn Arg Phe Thr 
    210                 215                 220                 
Thr Thr Leu Asn Asp Phe Asn Leu Val Ala Met Lys Tyr Asn Tyr Glu 
225                 230                 235                 240 
Pro Leu Thr Gln Asp His Val Asp Ile Leu Gly Pro Leu Ser Ala Gln 
                245                 250                 255     
Thr Gly Ile Ala Val Leu Asp Met Cys Ala Ser Leu Lys Glu Leu Leu 
            260                 265                 270         
Gln Asn Gly Met Asn Gly Arg Thr Ile Leu Gly Ser Ala Leu Leu Glu 
        275                 280                 285             
Asp Glu Phe Thr Pro Phe Asp Val Val Arg Gln Cys Ser Gly Val Thr 
    290                 295                 300                 
Phe Gln 
305     

<210> 22
<211> 341
<212> PRT
<213> Artificial Sequence


<220> 
<223> Mpro protein (Mpro-L fusion protein)

<400> 22
Ser Gly Phe Arg Lys Met Ala Phe Pro Ser Gly Lys Val Glu Gly Cys 
1               5                   10                  15      
Met Val Gln Val Thr Cys Gly Thr Thr Thr Leu Asn Gly Leu Trp Leu 
            20                  25                  30          
Asp Asp Val Val Tyr Cys Pro Arg His Val Ile Cys Thr Ser Glu Asp 
        35                  40                  45              
Met Leu Asn Pro Asn Tyr Glu Asp Leu Leu Ile Arg Lys Ser Asn His 
    50                  55                  60                  
Asn Phe Leu Val Gln Ala Gly Asn Val Gln Leu Arg Val Ile Gly His 
65                  70                  75                  80  
Ser Met Gln Asn Cys Val Leu Lys Leu Lys Val Asp Thr Ala Asn Pro 
                85                  90                  95      
Lys Thr Pro Lys Tyr Lys Phe Val Arg Ile Gln Pro Gly Gln Thr Phe 
            100                 105                 110         
Ser Val Leu Ala Cys Tyr Asn Gly Ser Pro Ser Gly Val Tyr Gln Cys 
        115                 120                 125             
Ala Met Arg Pro Asn Phe Thr Ile Lys Gly Ser Phe Leu Asn Gly Ser 
    130                 135                 140                 
Cys Gly Ser Val Gly Phe Asn Ile Asp Tyr Asp Cys Val Ser Phe Cys 
145                 150                 155                 160 
Tyr Met His His Met Glu Leu Pro Thr Gly Val His Ala Gly Thr Asp 
                165                 170                 175     
Leu Glu Gly Asn Phe Tyr Gly Pro Phe Val Asp Arg Gln Thr Ala Gln 
            180                 185                 190         
Ala Ala Gly Thr Asp Thr Thr Ile Thr Val Asn Val Leu Ala Trp Leu 
        195                 200                 205             
Tyr Ala Ala Val Ile Asn Gly Asp Arg Trp Phe Leu Asn Arg Phe Thr 
    210                 215                 220                 
Thr Thr Leu Asn Asp Phe Asn Leu Val Ala Met Lys Tyr Asn Tyr Glu 
225                 230                 235                 240 
Pro Leu Thr Gln Asp His Val Asp Ile Leu Gly Pro Leu Ser Ala Gln 
                245                 250                 255     
Thr Gly Ile Ala Val Leu Asp Met Cys Ala Ser Leu Lys Glu Leu Leu 
            260                 265                 270         
Gln Asn Gly Met Asn Gly Arg Thr Ile Leu Gly Ser Ala Leu Leu Glu 
        275                 280                 285             
Asp Glu Phe Thr Pro Phe Asp Val Val Arg Gln Cys Ser Gly Val Thr 
    290                 295                 300                 
Phe Gln Gly Gly Gly Gly Gly Ser Ser Phe His Gln Leu Pro Ala Arg 
305                 310                 315                 320 
Ser Pro Leu Pro Gly Gly Gly Gly Ser Phe Tyr Pro Ser Tyr His Ser 
                325                 330                 335     
Thr Pro Gln Arg Pro 
            340     

<210> 23
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> BM1

<400> 23
gttaacagag atttacacag agg                                            23


<210> 24
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> BM2

<400> 24
gcagccctcc actcccatgg agg                                            23


<210> 25
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> fdx promoter

<400> 25
attactttaa aaattaataa aaacatggta aaatataaat cgtataaagt tgtgtaattt     60

ttaaggaggt gtgttacat                                                  79


<210> 26
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> nprM3

<400> 26
Met Lys Ser Lys Lys Leu Leu Ala Thr Val Leu Ser Ala Val Ile Thr 
1               5                   10                  15      
Leu Ser Thr Val Ser Ala Val Tyr Ala 
            20                  25  

<210> 27
<211> 35
<212> PRT
<213> Artificial Sequence


<220> 
<223> L1-2

<400> 27
Gly Gly Gly Gly Gly Ser Ser Phe His Gln Leu Pro Ala Arg Ser Pro 
1               5                   10                  15      
Leu Pro Gly Gly Gly Gly Ser Phe Tyr Pro Ser Tyr His Ser Thr Pro 
            20                  25                  30          
Gln Arg Pro 
        35  

<210> 28
<211> 895
<212> DNA
<213> Artificial Sequence


<220> 
<223> PptbnprM3-RBD1

<400> 28
taagtcagca gaaagtataa tgagaaaata taaaatataa ataattttct aaaaaactta      60

acttcatgtg aaaagtttgt taaaatataa atgagcacgt taatcattta acatagataa     120

ttaaatagta aaagggagtg tacgaccaat gaaaagtaaa aaattattag ctacagtgct     180

aagtgctgta atcactcttt ctactgtttc tgcagtttat gctagagttc aaccaacaga     240

gtctatagtg agatttccta atattacaaa tctttgccca ttcggtgagg tattcaacgc     300

tacaaggttt gcaagtgttt acgcttggaa taggaaaaga atttctaatt gtgttgctga     360

ttattcagta ttatacaata gtgcttcatt ctcaacattt aagtgttatg gagtaagtcc     420

aacaaagttg aacgatttat gtttcacaaa tgtatatgct gatagtttcg tgattagggg     480

agatgaagta agacaaattg cacctggtca gacaggaaaa attgctgact acaattacaa     540

gttgcctgat gattttacag gatgtgtaat agcatggaac agtaacaact tagatagtaa     600

ggtgggagga aactataatt acttatacag actattcagg aaatcaaact taaaaccttt     660

tgaaagagac atatcaacag aaatatacca ggctggtagt acaccatgca atggtgtgga     720

aggatttaat tgttattttc ctcttcaatc atacggtttt caacctacta acggtgtagg     780

ataccaacct tatagggtag tggtgttgtc atttgagctt ttacacgcac ctgctactgt     840

gtgtggacct aagaagtcta ctaatctagt gaagaacaaa tgcgtaaact tttaa          895


<210> 29
<211> 826
<212> DNA
<213> Artificial Sequence


<220> 
<223> PfdxnprM3-RBD1

<400> 29
attactttaa aaattaataa aaacatggta aaatataaat cgtataaagt tgtgtaattt      60

ttaaggaggt gtgttacata tgaaaagtaa aaaattatta gctacagtgc taagtgctgt     120

aatcactctt tctactgttt ctgcagttta tgctagagtt caaccaacag agtctatagt     180

gagatttcct aatattacaa atctttgccc attcggtgag gtattcaacg ctacaaggtt     240

tgcaagtgtt tacgcttgga ataggaaaag aatttctaat tgtgttgctg attattcagt     300

attatacaat agtgcttcat tctcaacatt taagtgttat ggagtaagtc caacaaagtt     360

gaacgattta tgtttcacaa atgtatatgc tgatagtttc gtgattaggg gagatgaagt     420

aagacaaatt gcacctggtc agacaggaaa aattgctgac tacaattaca agttgcctga     480

tgattttaca ggatgtgtaa tagcatggaa cagtaacaac ttagatagta aggtgggagg     540

aaactataat tacttataca gactattcag gaaatcaaac ttaaaacctt ttgaaagaga     600

catatcaaca gaaatatacc aggctggtag tacaccatgc aatggtgtgg aaggatttaa     660

ttgttatttt cctcttcaat catacggttt tcaacctact aacggtgtag gataccaacc     720

ttatagggta gtggtgttgt catttgagct tttacacgca cctgctactg tgtgtggacc     780

taagaagtct actaatctag tgaagaacaa atgcgtaaac ttttaa                    826


<210> 30
<211> 928
<212> DNA
<213> Artificial Sequence


<220> 
<223> PfdxnprM3-RBD1-L

<400> 30
attactttaa aaattaataa aaacatggta aaatataaat cgtataaagt tgtgtaattt      60

ttaaggaggt gtgttacata tgaaaagtaa aaaattatta gctacagtgc taagtgctgt     120

aatcactctt tctactgttt ctgcagttta tgctagagtt caaccaacag agtctatagt     180

gagatttcct aatattacaa atctttgccc attcggtgag gtattcaacg ctacaaggtt     240

tgcaagtgtt tacgcttgga ataggaaaag aatttctaat tgtgttgctg attattcagt     300

attatacaat agtgcttcat tctcaacatt taagtgttat ggagtaagtc caacaaagtt     360

gaacgattta tgtttcacaa atgtatatgc tgatagtttc gtgattaggg gagatgaagt     420

aagacaaatt gcacctggtc agacaggaaa aattgctgac tacaattaca agttgcctga     480

tgattttaca ggatgtgtaa tagcatggaa cagtaacaac ttagatagta aggtgggagg     540

aaactataat tacttataca gactattcag gaaatcaaac ttaaaacctt ttgaaagaga     600

catatcaaca gaaatatacc aggctggtag tacaccatgc aatggtgtgg aaggatttaa     660

ttgttatttt cctcttcaat catacggttt tcaacctact aacggtgtag gataccaacc     720

ttatagggta gtggtgttgt catttgagct tttacacgca cctgctactg tgtgtggacc     780

taagaagtct actaatctag tgaagaacaa atgcgtaaac tttggtggag gaggttcatc     840

atttcatcaa ttaccagctc gttcaccatt accaggaggt ggtggatcat tttatccatc     900

atatcattca actccacaac gtccataa                                        928


<210> 31
<211> 1411
<212> DNA
<213> Artificial Sequence


<220> 
<223> PfdxnprM3-NC

<400> 31
attactttaa aaattaataa aaacatggta aaatataaat cgtataaagt tgtgtaattt      60

ttaaggaggt gtgttacata tgaaaagtaa aaaattatta gctacagtgc taagtgctgt     120

aatcactctt tctactgttt ctgcagttta tgcttctgat aatggacccc aaaatcagag     180

aaatgcaccc aggattacat ttggtggacc ctcagattca actggcagta accagaatgg     240

agaacgtagt ggggctagat caaaacaacg taggccccaa ggtttaccca ataatactgc     300

ctcttggttc accgctctta ctcaacatgg caaggaagat cttaaattcc ctagaggaca     360

aggcgttcca attaacacca atagcagtcc agatgaccaa attggctact accgtagagc     420

taccagaagg attcgtggtg gtgacggtaa aatgaaagat cttagtccaa gatggtattt     480

ctactaccta ggaactgggc cagaagctgg acttccctat ggtgctaaca aagacggcat     540

tatatgggtt gcaactgagg gagccttgaa tacaccaaaa gatcacattg gcaccaggaa     600

tcctgctaac aatgctgcaa tcgtgctaca acttcctcaa ggaacaacat tgccaaaagg     660

cttctacgca gaagggagca gaggcggcag tcaagcctct tctcgttcct catcacgtag     720

taggaacagt tcaagaaatt caactccagg cagcagtagg ggaacttctc ctgctagaat     780

ggctggcaat ggcggtgatg ctgctcttgc tttgctattg cttgacagat tgaaccagct     840

tgagagcaaa atgtctggta aaggccaaca acaacaaggc caaactgtga ctaagaaatc     900

tgctgctgag gcttctaaga agcctaggca aaaacgtact gccactaaag catacaatgt     960

aacacaagct ttcggcagac gtggtccaga acaaacccaa ggaaattttg gggaccagga    1020

actaattaga caaggaactg attacaaaca ttggcctcaa attgcacaat ttgcccccag    1080

cgcttcagca ttcttcggaa tgtctcgtat tggcatggaa gttacacctt ctggaacatg    1140

gttgacctac acaggtgcca ttaaattgga tgacaaagat ccaaatttca aagatcaagt    1200

gattttgctt aataagcata ttgacgcata caaaacattc ccaccaacag agcctaaaaa    1260

ggacaaaaag aagaaggctg atgaaactca agccttacca cagagacaga agaaacagca    1320

aactgtgact cttcttcctg ctgcagattt ggatgatttc tccaaacaat tgcaacaatc    1380

catgagcagt gctgactcaa ctcaggccta a                                   1411


<210> 32
<211> 1513
<212> DNA
<213> Artificial Sequence


<220> 
<223> PfdxnprM3-NC-L

<400> 32
attactttaa aaattaataa aaacatggta aaatataaat cgtataaagt tgtgtaattt      60

ttaaggaggt gtgttacata tgaaaagtaa aaaattatta gctacagtgc taagtgctgt     120

aatcactctt tctactgttt ctgcagttta tgcttctgat aatggacccc aaaatcagag     180

aaatgcaccc aggattacat ttggtggacc ctcagattca actggcagta accagaatgg     240

agaacgtagt ggggctagat caaaacaacg taggccccaa ggtttaccca ataatactgc     300

ctcttggttc accgctctta ctcaacatgg caaggaagat cttaaattcc ctagaggaca     360

aggcgttcca attaacacca atagcagtcc agatgaccaa attggctact accgtagagc     420

taccagaagg attcgtggtg gtgacggtaa aatgaaagat cttagtccaa gatggtattt     480

ctactaccta ggaactgggc cagaagctgg acttccctat ggtgctaaca aagacggcat     540

tatatgggtt gcaactgagg gagccttgaa tacaccaaaa gatcacattg gcaccaggaa     600

tcctgctaac aatgctgcaa tcgtgctaca acttcctcaa ggaacaacat tgccaaaagg     660

cttctacgca gaagggagca gaggcggcag tcaagcctct tctcgttcct catcacgtag     720

taggaacagt tcaagaaatt caactccagg cagcagtagg ggaacttctc ctgctagaat     780

ggctggcaat ggcggtgatg ctgctcttgc tttgctattg cttgacagat tgaaccagct     840

tgagagcaaa atgtctggta aaggccaaca acaacaaggc caaactgtga ctaagaaatc     900

tgctgctgag gcttctaaga agcctaggca aaaacgtact gccactaaag catacaatgt     960

aacacaagct ttcggcagac gtggtccaga acaaacccaa ggaaattttg gggaccagga    1020

actaattaga caaggaactg attacaaaca ttggcctcaa attgcacaat ttgcccccag    1080

cgcttcagca ttcttcggaa tgtctcgtat tggcatggaa gttacacctt ctggaacatg    1140

gttgacctac acaggtgcca ttaaattgga tgacaaagat ccaaatttca aagatcaagt    1200

gattttgctt aataagcata ttgacgcata caaaacattc ccaccaacag agcctaaaaa    1260

ggacaaaaag aagaaggctg atgaaactca agccttacca cagagacaga agaaacagca    1320

aactgtgact cttcttcctg ctgcagattt ggatgatttc tccaaacaat tgcaacaatc    1380

catgagcagt gctgactcaa ctcaggccgg tggaggaggt tcatcatttc atcaattacc    1440

agctcgttca ccattaccag gaggtggtgg atcattttat ccatcatatc attcaactcc    1500

acaacgtcca taa                                                       1513


