                         SEQUENCE LISTING

<110>  Ingenza Ltd
 
<120>  Endotoxin-Free Production of Recombinant Subunit Vaccine 
       Component

<130>  013001P0019

<150>  63/150,732
<151>  2021-02-18

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  1101
<212>  DNA
<213>  Partial pCVD032 Plasmid

<400>  1
atgaaaaaaa aactagcagc aggattaaca gcaagcgcca ttgttggcac aacacttgtc       60

gtcacaccgg ccgaagccgg aagctccgtc acaacactta gcggactttc cggcgaacaa      120

ggcccgtccg gcgacatgac aacagaagag gacagcgcca cacacatcaa gttctccaag      180

cgcgatgagg atggccgcga acttgctggc gctacaatgg aacttcgcga tagctccggc      240

aaaacgatct ccacgtggat ctccgacggc cacgtcaagg acttttatct ttatccgggc      300

aaatacacgt tcgtcgagac agccgctcca gatggctatg aagtcgccac gccgatcgag      360

ttcacggtca acgaggatgg acaagtcaca gtcgatggcg aagctacaga aggcgatgcc      420

catacgggcg gcagcggagg aagcggaggc agcggaggct ccatgaagat ggaggagctt      480

ttcaagaagc acaagatcgt cgccgttctt agagccaact ccgtcgagga agccaagaag      540

aaagctcttg ccgttttcct tggcggcgtc catcttatcg aaatcacatt cacggtcccg      600

gatgccgata cggtcatcaa ggagctgtcc tttcttaagg agatgggcgc cattatcggc      660

gccggcacag ttacgagcgt cgaacaagct cgcaaagccg tcgaaagcgg cgctgagttt      720

atcgtctccc cgcaccttga tgaagagatc agccagttcg ccaaggagaa aggcgtcttc      780

tacatgccgg gcgttatgac gccgacggaa ctggttaaag ccatgaagct gggccacacg      840

attctgaaac tgtttccggg cgaggtcgtc ggaccgcagt ttgtcaaagc tatgaagggc      900

ccgttcccga atgttaagtt cgttccgacg ggcggagtca atcttgacaa cgtctgcgag      960

tggttcaaag ctggagttct tgccgttggc gttggaagcg cccttgtcaa aggaacgcca     1020

gtcgaagttg ccgagaaggc caaagccttc gtcgagaaaa tccgcggctg tacagaaggc     1080

agcggcgaac cagaggccta a                                               1101


<210>  2
<211>  78
<212>  DNA
<213>  Secretion Signal (LytF)

<400>  2
atgaaaaaaa aactagcagc aggattaaca gcaagcgcca ttgttggcac aacacttgtc       60

gtcacaccgg ccgaagcc                                                     78


<210>  3
<211>  360
<212>  DNA
<213>  SpyCatcher003

<400>  3
ggaagctccg tcacaacact tagcggactt tccggcgaac aaggcccgtc cggcgacatg       60

acaacagaag aggacagcgc cacacacatc aagttctcca agcgcgatga ggatggccgc      120

gaacttgctg gcgctacaat ggaacttcgc gatagctccg gcaaaacgat ctccacgtgg      180

atctccgacg gccacgtcaa ggacttttat ctttatccgg gcaaatacac gttcgtcgag      240

acagccgctc cagatggcta tgaagtcgcc acgccgatcg agttcacggt caacgaggat      300

ggacaagtca cagtcgatgg cgaagctaca gaaggcgatg cccatacggg cggcagcgga      360


<210>  4
<211>  24
<212>  DNA
<213>  Linker 1

<400>  4
ggaagcggag gcagcggagg ctcc                                              24


<210>  5
<211>  615
<212>  DNA
<213>  Mi3 Monomer

<400>  5
atgaagatgg aggagctttt caagaagcac aagatcgtcg ccgttcttag agccaactcc       60

gtcgaggaag ccaagaagaa agctcttgcc gttttccttg gcggcgtcca tcttatcgaa      120

atcacattca cggtcccgga tgccgatacg gtcatcaagg agctgtcctt tcttaaggag      180

atgggcgcca ttatcggcgc cggcacagtt acgagcgtcg aacaagctcg caaagccgtc      240

gaaagcggcg ctgagtttat cgtctccccg caccttgatg aagagatcag ccagttcgcc      300

aaggagaaag gcgtcttcta catgccgggc gttatgacgc cgacggaact ggttaaagcc      360

atgaagctgg gccacacgat tctgaaactg tttccgggcg aggtcgtcgg accgcagttt      420

gtcaaagcta tgaagggccc gttcccgaat gttaagttcg ttccgacggg cggagtcaat      480

cttgacaacg tctgcgagtg gttcaaagct ggagttcttg ccgttggcgt tggaagcgcc      540

cttgtcaaag gaacgccagt cgaagttgcc gagaaggcca aagccttcgt cgagaaaatc      600

cgcggctgta cagaa                                                       615


<210>  6
<211>  9
<212>  DNA
<213>  Linker 2

<400>  6
ggcagcggc                                                                9


<210>  7
<211>  15
<212>  DNA
<213>  C-tag

<400>  7
gaaccagagg cctaa                                                        15


<210>  8
<211>  87
<212>  DNA
<213>  Signal Peptide 1 (DacC)

<400>  8
atgaaaaaaa gcatcaagct gtacgtcgcc gtccttcttc tgtttgttgt cgccagcgtc       60

ccgtatatgc atcaagccgc ccttgcc                                           87


<210>  9
<211>  96
<212>  DNA
<213>  Signal Peptide 2 (PhoB)

<400>  9
atgaagaagt tcccgaagaa actgctgccg atcgccgttc ttagcagcat cgcctttagc       60

agccttgcta gcggcagcgt tccggaagct agcgcc                                 96


<210>  10
<211>  87
<212>  DNA
<213>  Signal Peptide 3 (BglC)

<400>  10
atgaagcgca gcatcagcat cttcatcacg tgtcttctga tcacgcttct tacaatgggc       60

ggcatgcttg ctagcccagc tagcgcc                                           87


<210>  11
<211>  81
<212>  DNA
<213>  Signal Peptide 4 (YlqB)

<400>  11
atgaagaaga tcggacttct gttcatgctg tgccttgccg ccctttttac aatcggcttt       60

ccggcccagc aagccgatgc c                                                 81


<210>  12
<211>  78
<212>  DNA
<213>  Signal Peptide 5 (LytF)

<400>  12
atgaaaaaaa aactggccgc cggacttaca gctagcgcca ttgttggcac aacacttgtc       60

gtcacaccgg ccgaagcc                                                     78


<210>  13
<211>  1100
<212>  DNA
<213>  Codon Usage 1

<400>  13
atgaagaaga agctcgcggc ggggctcacc gcgagcgcca ttgttggcac aacacttgtc       60

gtcacaccgg ccgaagccgg aagctccgtc acaacactta gcggactttc cggcgaacaa      120

ggcccgtccg gcgacatgac aacagaagag gacagcgcca cacacatcaa gttctccaag      180

cgcgatgagg atggccgcga acttgctggc gctacaatgg aacttcgcga tagctccggc      240

aaaacgatct ccacgtggat ctccgacggc cacgtcaagg acttttatct ttatccgggc      300

aaatacacgt tcgtcgagac agccgctcca gatggctatg aagtcgccac gccgatcgag      360

ttcacggtca acgaggatgg acaagtcaca gtcgatggcg aagctacaga aggcgatgcc      420

atacgggcgg cagcggagga agcggaggca gcggaggctc catgaagatg gaggagcttt      480

tcaagaagca caagatcgtc gccgttctta gagccaactc cgtcgaggaa gccaagaaga      540

aagctcttgc cgttttcctt ggcggcgtcc atcttatcga aatcacattc acggtcccgg      600

atgccgatac ggtcatcaag gagctgtcct ttcttaagga gatgggcgcc attatcggcg      660

ccggcacagt tacgagcgtc gaacaagctc gcaaagccgt cgaaagcggc gctgagttta      720

tcgtctcccc gcaccttgat gaagagatca gccagttcgc caaggagaaa ggcgtcttct      780

acatgccggg cgttatgacg ccgacggaac tggttaaagc catgaagctg ggccacacga      840

ttctgaaact gtttccgggc gaggtcgtcg gaccgcagtt tgtcaaagct atgaagggcc      900

cgttcccgaa tgttaagttc gttccgacgg gcggagtcaa tcttgacaac gtctgcgagt      960

ggttcaaagc tggagttctt gccgttggcg ttggaagcgc ccttgtcaaa ggaacgccag     1020

tcgaagttgc cgagaaggcc aaagccttcg tcgagaaaat ccgcggctgt acagaaggca     1080

gcggcgaacc agaggcctaa                                                 1100


<210>  14
<211>  1100
<212>  DNA
<213>  Codon Usage 2

<400>  14
atgaagaaaa aattagccgc tgggctcact gcgagcgcca ttgttggcac aacacttgtc       60

gtcacaccgg ccgaagccgg aagctccgtc acaacactta gcggactttc cggcgaacaa      120

ggcccgtccg gcgacatgac aacagaagag gacagcgcca cacacatcaa gttctccaag      180

cgcgatgagg atggccgcga acttgctggc gctacaatgg aacttcgcga tagctccggc      240

aaaacgatct ccacgtggat ctccgacggc cacgtcaagg acttttatct ttatccgggc      300

aaatacacgt tcgtcgagac agccgctcca gatggctatg aagtcgccac gccgatcgag      360

ttcacggtca acgaggatgg acaagtcaca gtcgatggcg aagctacaga aggcgatgcc      420

atacgggcgg cagcggagga agcggaggca gcggaggctc catgaagatg gaggagcttt      480

tcaagaagca caagatcgtc gccgttctta gagccaactc cgtcgaggaa gccaagaaga      540

aagctcttgc cgttttcctt ggcggcgtcc atcttatcga aatcacattc acggtcccgg      600

atgccgatac ggtcatcaag gagctgtcct ttcttaagga gatgggcgcc attatcggcg      660

ccggcacagt tacgagcgtc gaacaagctc gcaaagccgt cgaaagcggc gctgagttta      720

tcgtctcccc gcaccttgat gaagagatca gccagttcgc caaggagaaa ggcgtcttct      780

acatgccggg cgttatgacg ccgacggaac tggttaaagc catgaagctg ggccacacga      840

ttctgaaact gtttccgggc gaggtcgtcg gaccgcagtt tgtcaaagct atgaagggcc      900

cgttcccgaa tgttaagttc gttccgacgg gcggagtcaa tcttgacaac gtctgcgagt      960

ggttcaaagc tggagttctt gccgttggcg ttggaagcgc ccttgtcaaa ggaacgccag     1020

tcgaagttgc cgagaaggcc aaagccttcg tcgagaaaat ccgcggctgt acagaaggca     1080

gcggcgaacc agaggcctaa                                                 1100


<210>  15
<211>  1100
<212>  DNA
<213>  Codon Usage 3

<400>  15
atgaaaaaaa aactagcagc aggattaaca gcaagcgcca ttgttggcac aacacttgtc       60

gtcacaccgg ccgaagccgg aagctccgtc acaacactta gcggactttc cggcgaacaa      120

ggcccgtccg gcgacatgac aacagaagag gacagcgcca cacacatcaa gttctccaag      180

cgcgatgagg atggccgcga acttgctggc gctacaatgg aacttcgcga tagctccggc      240

aaaacgatct ccacgtggat ctccgacggc cacgtcaagg acttttatct ttatccgggc      300

aaatacacgt tcgtcgagac agccgctcca gatggctatg aagtcgccac gccgatcgag      360

ttcacggtca acgaggatgg acaagtcaca gtcgatggcg aagctacaga aggcgatgcc      420

atacgggcgg cagcggagga agcggaggca gcggaggctc catgaagatg gaggagcttt      480

tcaagaagca caagatcgtc gccgttctta gagccaactc cgtcgaggaa gccaagaaga      540

aagctcttgc cgttttcctt ggcggcgtcc atcttatcga aatcacattc acggtcccgg      600

atgccgatac ggtcatcaag gagctgtcct ttcttaagga gatgggcgcc attatcggcg      660

ccggcacagt tacgagcgtc gaacaagctc gcaaagccgt cgaaagcggc gctgagttta      720

tcgtctcccc gcaccttgat gaagagatca gccagttcgc caaggagaaa ggcgtcttct      780

acatgccggg cgttatgacg ccgacggaac tggttaaagc catgaagctg ggccacacga      840

ttctgaaact gtttccgggc gaggtcgtcg gaccgcagtt tgtcaaagct atgaagggcc      900

cgttcccgaa tgttaagttc gttccgacgg gcggagtcaa tcttgacaac gtctgcgagt      960

ggttcaaagc tggagttctt gccgttggcg ttggaagcgc ccttgtcaaa ggaacgccag     1020

tcgaagttgc cgagaaggcc aaagccttcg tcgagaaaat ccgcggctgt acagaaggca     1080

gcggcgaacc agaggcctaa                                                 1100


<210>  16
<211>  1100
<212>  DNA
<213>  Codon Usage 4

<400>  16
atgaaaaaaa aactggccgc cggacttaca gctagcgcca ttgttggcac aacacttgtc       60

gtcacaccgg ccgaagccgg aagctccgtc acaacactta gcggactttc cggcgaacaa      120

ggcccgtccg gcgacatgac aacagaagag gacagcgcca cacacatcaa gttctccaag      180

cgcgatgagg atggccgcga acttgctggc gctacaatgg aacttcgcga tagctccggc      240

aaaacgatct ccacgtggat ctccgacggc cacgtcaagg acttttatct ttatccgggc      300

aaatacacgt tcgtcgagac agccgctcca gatggctatg aagtcgccac gccgatcgag      360

ttcacggtca acgaggatgg acaagtcaca gtcgatggcg aagctacaga aggcgatgcc      420

atacgggcgg cagcggagga agcggaggca gcggaggctc catgaagatg gaggagcttt      480

tcaagaagca caagatcgtc gccgttctta gagccaactc cgtcgaggaa gccaagaaga      540

aagctcttgc cgttttcctt ggcggcgtcc atcttatcga aatcacattc acggtcccgg      600

atgccgatac ggtcatcaag gagctgtcct ttcttaagga gatgggcgcc attatcggcg      660

ccggcacagt tacgagcgtc gaacaagctc gcaaagccgt cgaaagcggc gctgagttta      720

tcgtctcccc gcaccttgat gaagagatca gccagttcgc caaggagaaa ggcgtcttct      780

acatgccggg cgttatgacg ccgacggaac tggttaaagc catgaagctg ggccacacga      840

ttctgaaact gtttccgggc gaggtcgtcg gaccgcagtt tgtcaaagct atgaagggcc      900

cgttcccgaa tgttaagttc gttccgacgg gcggagtcaa tcttgacaac gtctgcgagt      960

ggttcaaagc tggagttctt gccgttggcg ttggaagcgc ccttgtcaaa ggaacgccag     1020

tcgaagttgc cgagaaggcc aaagccttcg tcgagaaaat ccgcggctgt acagaaggca     1080

gcggcgaacc agaggcctaa                                                 1100


