<110>    SNU R&DB Foundation
         Shaperon

<120>    Single domain antibodies against CD47 and use thereof

<130>    PCT2022-002

<150>    KR 10-2021-0022805
<151>    2021-02-19

<160>    19

<170>    KoPatentIn 3.0

<210>    1
<211>    123
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01


<400>    1
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Asn Leu Ser Cys Ala Val Ser Gly Leu Thr Phe Phe Arg Lys
             20                  25                  30 

Tyr Ala Val Gly Trp Ala Arg Gln Val Pro Gly Lys Gly Leu Glu Arg
         35                  40                  45 

Val Ser Arg Ile Tyr Ser Asp Arg Arg Ile Glu Tyr Leu Asp Ala Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Thr Gly Leu Arg Val Gly Leu Thr Gly Trp Asp Glu Tyr Asp Tyr
            100                 105                 110 

Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser
        115                 120            


<210>    2
<211>    125
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#02


<400>    2
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Ser Thr Leu Asn Tyr Tyr
             20                  25                  30 

Ile Ile Ser Trp Phe Arg Gln Thr Pro Gly Lys Glu Arg Glu Gly Val
         35                  40                  45 

Ser Cys Ile Gly Ser Ser Gly Ala Gly Thr Asn Tyr Ala Ala Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Gln Asn Thr Val Tyr
 65                  70                  75                  80 

Leu Phe Met Asn Asp Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Ala Arg Pro Phe Ser Phe Ser Pro Phe Cys Thr Phe Ala Gly Met
            100                 105                 110 

Lys Asp Trp Gly Lys Gly Thr Leu Val Thr Val Ser Ser
        115                 120                 125


<210>    3
<211>    9
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01_CDR1


<400>    3
Gly Leu Thr Phe Phe Arg Lys Tyr Ala
  1               5                


<210>    4
<211>    7
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01_CDR2


<400>    4
Ile Tyr Ser Asp Arg Arg Ile
  1               5        


<210>    5
<211>    16
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01_CDR3


<400>    5
Ala Thr Gly Leu Arg Val Gly Leu Thr Gly Trp Asp Glu Tyr Asp Tyr
  1               5                  10                  15 




<210>    6
<211>    8
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#02_CDR1


<400>    6
Gly Ser Thr Leu Asn Tyr Tyr Ile
  1               5            


<210>    7
<211>    8
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#02_CDR2


<400>    7
Ile Gly Ser Ser Gly Ala Gly Thr
  1               5            


<210>    8
<211>    18
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#02_CDR3


<400>    8
Ala Ala Arg Pro Phe Ser Phe Ser Pro Phe Cys Thr Phe Ala Gly Met
  1               5                  10                  15 

Lys Asp
       


<210>    9
<211>    360
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01-IgG1


<400>    9
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Asn Leu Ser Cys Ala Val Ser Gly Leu Thr Phe Phe Arg Lys
             20                  25                  30 

Tyr Ala Val Gly Trp Ala Arg Gln Val Pro Gly Lys Gly Leu Glu Arg
         35                  40                  45 

Val Ser Arg Ile Tyr Ser Asp Arg Arg Ile Glu Tyr Leu Asp Ala Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Thr Gly Leu Arg Val Gly Leu Thr Gly Trp Asp Glu Tyr Asp Tyr
            100                 105                 110 

Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly Pro Gly Gly Pro
        115                 120                 125 

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala
    130                 135                 140 

Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro
145                 150                 155                 160 

Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val
                165                 170                 175 

Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val
            180                 185                 190 

Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln
        195                 200                 205 

Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln
    210                 215                 220 

Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala
225                 230                 235                 240 

Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro
                245                 250                 255 

Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr
            260                 265                 270 

Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser
        275                 280                 285 

Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr
    290                 295                 300 

Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr
305                 310                 315                 320 

Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe
                325                 330                 335 

Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys
            340                 345                 350 

Ser Leu Ser Leu Ser Pro Gly Lys
        355                 360


<210>    10
<211>    362
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#02-IgG1


<400>    10
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Ser Thr Leu Asn Tyr Tyr
             20                  25                  30 

Ile Ile Ser Trp Phe Arg Gln Thr Pro Gly Lys Glu Arg Glu Gly Val
         35                  40                  45 

Ser Cys Ile Gly Ser Ser Gly Ala Gly Thr Asn Tyr Ala Ala Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Gln Asn Thr Val Tyr
 65                  70                  75                  80 

Leu Phe Met Asn Asp Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Ala Arg Pro Phe Ser Phe Ser Pro Phe Cys Thr Phe Ala Gly Met
            100                 105                 110 

Lys Asp Trp Gly Lys Gly Thr Leu Val Thr Val Ser Ser Gly Pro Gly
        115                 120                 125 

Gly Pro Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys
    130                 135                 140 

Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro
145                 150                 155                 160 

Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys
                165                 170                 175 

Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
            180                 185                 190 

Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
        195                 200                 205 

Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu
    210                 215                 220 

His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn
225                 230                 235                 240 

Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
                245                 250                 255 

Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
            260                 265                 270 

Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
        275                 280                 285 

Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
    290                 295                 300 

Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
305                 310                 315                 320 

Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
                325                 330                 335 

Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
            340                 345                 350 

Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        355                 360        


<210>    11
<211>    25
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01_FR1


<400>    11
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Asn Leu Ser Cys Ala Val Ser
             20                  25


<210>    12
<211>    17
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01_FR2


<400>    12
Val Gly Trp Ala Arg Gln Val Pro Gly Lys Gly Leu Glu Arg Val Ser
  1               5                  10                  15 

Arg
   


<210>    13
<211>    38
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01_FR3


<400>    13
Glu Tyr Leu Asp Ala Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn
  1               5                  10                  15 

Ala Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp
             20                  25                  30 

Thr Ala Val Tyr Tyr Cys
         35            


<210>    14
<211>    11
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#01_FR4


<400>    14
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser
  1               5                  10    


<210>    15
<211>    25
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#02_FR1


<400>    15
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Thr Ala Ser
             20                  25


<210>    16
<211>    17
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#02_FR2


<400>    16
Ile Ser Trp Phe Arg Gln Thr Pro Gly Lys Glu Arg Glu Gly Val Ser
  1               5                  10                  15 

Cys
   


<210>    17
<211>    38
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#02_FR3


<400>    17
Asn Tyr Ala Ala Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp
  1               5                  10                  15 

Ala Gln Asn Thr Val Tyr Leu Phe Met Asn Asp Leu Lys Pro Glu Asp
             20                  25                  30 

Thr Ala Val Tyr Tyr Cys
         35            


<210>    18
<211>    11
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb#02_FR4


<400>    18
Trp Gly Lys Gly Thr Leu Val Thr Val Ser Ser
  1               5                  10    


<210>    19
<211>    357
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CD47 Nb-IgG4


<400>    19
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Asn Leu Ser Cys Ala Val Ser Gly Leu Thr Phe Phe Arg Lys
             20                  25                  30 

Tyr Ala Val Gly Trp Ala Arg Gln Val Pro Gly Lys Gly Leu Glu Arg
         35                  40                  45 

Val Ser Arg Ile Tyr Ser Asp Arg Arg Ile Glu Tyr Leu Asp Ala Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Thr Gly Leu Arg Val Gly Leu Thr Gly Trp Asp Glu Tyr Asp Tyr
            100                 105                 110 

Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly Pro Gly Gly Pro
        115                 120                 125 

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe
    130                 135                 140 

Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
145                 150                 155                 160 

Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
                165                 170                 175 

Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
            180                 185                 190 

Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
        195                 200                 205 

Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
    210                 215                 220 

Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser
225                 230                 235                 240 

Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
                245                 250                 255 

Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln
            260                 265                 270 

Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
        275                 280                 285 

Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
    290                 295                 300 

Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu
305                 310                 315                 320 

Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser
                325                 330                 335 

Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
            340                 345                 350 

Leu Ser Pro Gly Lys
        355        
