                         SEQUENCE LISTING

<110>  Aptevo Research and Development LLC
 
<120>  COMPOSITIONS COMPRISING 4-1BB AND OX40 BINDING PROTEINS AND 
       METHODS OF USE

<130>  4897.006PC01

<150>  US 63/150,475
<151>  2021-02-17

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  4-1BB Heavy Chain CDR 1

<400>  1

Gly Tyr Thr Phe Thr Ser Tyr Trp 
1               5               


<210>  2
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  4-1BB Heavy Chain CDR 2

<400>  2

Ile Tyr Pro Gly Ser Ser Thr Thr 
1               5               


<210>  3
<211>  14
<212>  PRT
<213>  artificial sequence

<220>
<223>  4-1BB Heavy Chain CDR 3

<400>  3

Ala Ser Phe Ser Asp Gly Tyr Tyr Ala Tyr Ala Met Asp Tyr 
1               5                   10                  


<210>  4
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  4-1BB Light Chain CDR  1

<400>  4

Gln Asp Ile Ser Asn Tyr 
1               5       


<210>  5
<211>  3
<212>  PRT
<213>  artificial sequence

<220>
<223>  4-1BB Light Chain CDR  2

<400>  5

Tyr Thr Ser 
1           


<210>  6
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  4-1BB Light Chain CDR  3

<400>  6

Gln Gln Gly Tyr Thr Leu Pro Tyr Thr 
1               5                   


<210>  7
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  OX40 Heavy Chain CDR1

<400>  7

Gly Phe Thr Leu Ser Tyr Tyr Gly 
1               5               


<210>  8
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  OX40 Heavy Chain CDR2

<400>  8

Ile Ser His Asp Gly Ser Asp Lys 
1               5               


<210>  9
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  OX40 Heavy Chain CDR3

<400>  9

Ser Asn Asp Gln Phe Asp Pro 
1               5           


<210>  10
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  OX40 Light Chain CDR1

<400>  10

Asn Ile Gly Ser Lys Ser 
1               5       


<210>  11
<211>  3
<212>  PRT
<213>  artificial sequence

<220>
<223>  OX40 Light Chain CDR2

<400>  11

Asp Asp Ser 
1           


<210>  12
<211>  11
<212>  PRT
<213>  artificial sequence

<220>
<223>  OX40 Light Chain CDR3

<400>  12

Gln Val Trp Asp Ser Ser Ser Asp His Val Val 
1               5                   10      


<210>  13
<211>  727
<212>  PRT
<213>  artificial sequence

<220>
<223>  4-1BB x OX40 bispecific antibody

<400>  13

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Asn Ile Tyr Pro Ser Gly Gly Ser Thr Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Val Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Phe Ser Asp Gly Tyr Tyr Ala Tyr Ala Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val 
    130                 135                 140                 


Met Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala 
145                 150                 155                 160 


Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr Leu Asn Trp 
                165                 170                 175     


Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr Tyr Ala 
            180                 185                 190         


Ser Arg Arg His Thr Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser 
        195                 200                 205             


Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe 
    210                 215                 220                 


Ala Val Tyr Tyr Cys Gln Gln Gly Tyr Asn Leu Pro Tyr Thr Phe Gly 
225                 230                 235                 240 


Gln Gly Thr Lys Val Glu Ile Lys Glu Pro Lys Ser Ser Asp Lys Thr 
                245                 250                 255     


His Thr Cys Pro Pro Cys Pro Ala Pro Pro Ala Ala Ala Pro Ser Val 
            260                 265                 270         


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
        275                 280                 285             


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
    290                 295                 300                 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
305                 310                 315                 320 


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
                325                 330                 335     


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
            340                 345                 350         


Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
        355                 360                 365             


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
    370                 375                 380                 


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
385                 390                 395                 400 


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
                405                 410                 415     


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
            420                 425                 430         


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
        435                 440                 445             


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
    450                 455                 460                 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly Gly 
465                 470                 475                 480 


Gly Ser Pro Ser Ser Tyr Val Leu Thr Gln Pro Pro Ser Val Ser Val 
                485                 490                 495     


Ala Pro Gly Lys Thr Ala Arg Ile Thr Cys Gly Gly Asn Asn Ile Gly 
            500                 505                 510         


Ser Lys Ser Val Asn Trp Phe Gln Gln Lys Pro Gly Gln Ala Pro Val 
        515                 520                 525             


Leu Val Val Tyr Asp Asp Ser Gly Arg Pro Ser Gly Ile Pro Glu Arg 
    530                 535                 540                 


Phe Ser Gly Ser Thr Ser Gly Asn Thr Ala Thr Leu Thr Ile Ser Arg 
545                 550                 555                 560 


Val Glu Ala Gly Asp Glu Ala Asp Tyr Tyr Cys Gln Val Trp Asp Ser 
                565                 570                 575     


Ser Ser Asp His Val Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            580                 585                 590         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        595                 600                 605             


Gly Gly Gly Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val 
    610                 615                 620                 


Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
625                 630                 635                 640 


Leu Ser Tyr Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly 
                645                 650                 655     


Leu Glu Trp Val Ala Ala Ile Ser His Asp Gly Ser Asp Lys Tyr Tyr 
            660                 665                 670         


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
        675                 680                 685             


Asn Arg Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
    690                 695                 700                 


Val Tyr Tyr Cys Ser Asn Asp Gln Phe Asp Pro Trp Gly Gln Gly Thr 
705                 710                 715                 720 


Leu Val Thr Val Ser Ser Arg 
                725         


<210>  14
<211>  121
<212>  PRT
<213>  artificial sequence

<220>
<223>  4-1BB Variable Heavy Chain  Amino Acid Sequence

<400>  14

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Asn Ile Tyr Pro Ser Gly Gly Ser Thr Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Val Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Phe Ser Asp Gly Tyr Tyr Ala Tyr Ala Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120     


<210>  15
<211>  107
<212>  PRT
<213>  artificial sequence

<220>
<223>  4-1BB Variable Light Chain

<400>  15

Glu Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Arg Arg His Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Gly Tyr Asn Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  16
<211>  248
<212>  PRT
<213>  artificial sequence

<220>
<223>  scFv

<400>  16

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Asn Ile Tyr Pro Ser Gly Gly Ser Thr Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Val Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Phe Ser Asp Gly Tyr Tyr Ala Tyr Ala Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val 
    130                 135                 140                 


Met Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala 
145                 150                 155                 160 


Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr Leu Asn Trp 
                165                 170                 175     


Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr Tyr Ala 
            180                 185                 190         


Ser Arg Arg His Thr Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser 
        195                 200                 205             


Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe 
    210                 215                 220                 


Ala Val Tyr Tyr Cys Gln Gln Gly Tyr Asn Leu Pro Tyr Thr Phe Gly 
225                 230                 235                 240 


Gln Gly Thr Lys Val Glu Ile Lys 
                245             


<210>  17
<211>  114
<212>  PRT
<213>  artificial sequence

<220>
<223>  OX40 Variable Heavy Chain

<400>  17

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Leu Ser Tyr Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Ala Ile Ser His Asp Gly Ser Asp Lys Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Arg Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Asn Asp Gln Phe Asp Pro Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  18
<211>  108
<212>  PRT
<213>  artificial sequence

<220>
<223>  Variable Light Chain

<400>  18

Ser Tyr Val Leu Thr Gln Pro Pro Ser Val Ser Val Ala Pro Gly Lys 
1               5                   10                  15      


Thr Ala Arg Ile Thr Cys Gly Gly Asn Asn Ile Gly Ser Lys Ser Val 
            20                  25                  30          


Asn Trp Phe Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Val Tyr 
        35                  40                  45              


Asp Asp Ser Gly Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser Gly Ser 
    50                  55                  60                  


Thr Ser Gly Asn Thr Ala Thr Leu Thr Ile Ser Arg Val Glu Ala Gly 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Gln Val Trp Asp Ser Ser Ser Asp His 
                85                  90                  95      


Val Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105             


<210>  19
<211>  243
<212>  PRT
<213>  artificial sequence

<220>
<223>  OX40 scFv

<400>  19

Ser Tyr Val Leu Thr Gln Pro Pro Ser Val Ser Val Ala Pro Gly Lys 
1               5                   10                  15      


Thr Ala Arg Ile Thr Cys Gly Gly Asn Asn Ile Gly Ser Lys Ser Val 
            20                  25                  30          


Asn Trp Phe Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Val Tyr 
        35                  40                  45              


Asp Asp Ser Gly Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser Gly Ser 
    50                  55                  60                  


Thr Ser Gly Asn Thr Ala Thr Leu Thr Ile Ser Arg Val Glu Ala Gly 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Gln Val Trp Asp Ser Ser Ser Asp His 
                85                  90                  95      


Val Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gly Gly Gly 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
    130                 135                 140                 


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Leu Ser Tyr Tyr 
145                 150                 155                 160 


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
                165                 170                 175     


Ala Ala Ile Ser His Asp Gly Ser Asp Lys Tyr Tyr Ala Asp Ser Val 
            180                 185                 190         


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Arg Leu Tyr 
        195                 200                 205             


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
    210                 215                 220                 


Ser Asn Asp Gln Phe Asp Pro Trp Gly Gln Gly Thr Leu Val Thr Val 
225                 230                 235                 240 


Ser Ser Arg 
            


<210>  20
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  20

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15

<210>  21
<211>  4
<212>  PRT
<213>  artificial sequence

<220>
<223>  Linker


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  Wherein the sequence can be repeated between 1 to 5 times

<400>  21

Gly Gly Gly Gly Ser 
1               


