                         SEQUENCE LISTING

<110>  SUMAGEN CANADA INC.
       KANG, Chil-Yong et al.
 
<120>  RECOMBINANT VSV-SARS-COV-2 VACCINE

<130>  0195924.0008

<150>  US 63/156,109
<151>  2021-03-03

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  690
<212>  DNA
<213>  Vesicular stomatitis Indiana virus


<220>
<221>  SITE
<222>  (1)..(690)
<223>  VSV Indiana serotype

<400>  1
atgagttcct taaagaagat tctcggtctg aaggggaaag gtaagaaatc taagaaatta       60

gggatcgcac caccccctta tgaagaggac actaacatgg agtatgctcc gagcgctcca      120

attgacaaat cctattttgg agttgacgag atggacactc atgatccgca tcaattaaga      180

tatgagaaat tcttctttac agtgaaaatg acggttagat ctaatcgtcc gttcagaaca      240

tactcagatg tggcagccgc tgtatcccat tgggatcaca tgtacatcgg aatggcaggg      300

aaacgtccct tctacaagat cttggctttt ttgggttctt ctaatctaaa ggccactcca      360

gcggtattgg cagatcaagg tcaaccagag tatcacgctc actgtgaagg cagggcttat      420

ttgccacaca gaatggggaa gacccctccc atgctcaatg taccagagca cttcagaaga      480

ccattcaata taggtcttta caagggaacg gttgagctca caatgaccat ctacgatgat      540

gagtcactgg aagcagctcc tatgatctgg gatcatttca attcttccaa attttctgat      600

ttcagagata aggccttaat gtttggcctg attgtcgaga aaaaggcatc tggagcttgg      660

gtcctggatt ctgtcagcca cttcaaatga                                       690


<210>  2
<211>  690
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Mutant G21E/L111A/M51R

<400>  2
atgagttcct taaagaagat tctcggtctg aaggggaaag gtaagaaatc taagaaatta       60

gaaatcgcac caccccctta tgaagaggac actaacatgg agtatgctcc gagcgctcca      120

attgacaaat cctattttgg agttgacgag cgagacactc atgatccgca tcaattaaga      180

tatgagaaat tcttctttac agtgaaaatg acggttagat ctaatcgtcc gttcagaaca      240

tactcagatg tggcagccgc tgtatcccat tgggatcaca tgtacatcgg aatggcaggg      300

aaacgtccct tctacaagat cttggctttt gcaggttctt ctaatctaaa ggccactcca      360

gcggtattgg cagatcaagg tcaaccagag tatcacgctc actgtgaagg cagggcttat      420

ttgccacaca gaatggggaa gacccctccc atgctcaatg taccagagca cttcagaaga      480

ccattcaata taggtcttta caagggaacg gttgagctca caatgaccat ctacgatgat      540

gagtcactgg aagcagctcc tatgatctgg gatcatttca attcttccaa attttctgat      600

ttcagagata aggccttaat gtttggcctg attgtcgaga aaaaggcatc tggagcttgg      660

gtcctggatt ctgtcagcca cttcaaatga                                       690


<210>  3
<211>  229
<212>  PRT
<213>  Vesicular stomatitis Indiana virus


<220>
<221>  SITE
<222>  (1)..(229)
<223>  VSV Indiana serotype

<400>  3

Met Ser Ser Leu Lys Lys Ile Leu Gly Leu Lys Gly Lys Gly Lys Lys 
1               5                   10                  15      


Ser Lys Lys Leu Gly Ile Ala Pro Pro Pro Tyr Glu Glu Asp Thr Asn 
            20                  25                  30          


Met Glu Tyr Ala Pro Ser Ala Pro Ile Asp Lys Ser Tyr Phe Gly Val 
        35                  40                  45              


Asp Glu Met Asp Thr His Asp Pro His Gln Leu Arg Tyr Glu Lys Phe 
    50                  55                  60                  


Phe Phe Thr Val Lys Met Thr Val Arg Ser Asn Arg Pro Phe Arg Thr 
65                  70                  75                  80  


Tyr Ser Asp Val Ala Ala Ala Val Ser His Trp Asp His Met Tyr Ile 
                85                  90                  95      


Gly Met Ala Gly Lys Arg Pro Phe Tyr Lys Ile Leu Ala Phe Leu Gly 
            100                 105                 110         


Ser Ser Asn Leu Lys Ala Thr Pro Ala Val Leu Ala Asp Gln Gly Gln 
        115                 120                 125             


Pro Glu Tyr His Ala His Cys Glu Gly Arg Ala Tyr Leu Pro His Arg 
    130                 135                 140                 


Met Gly Lys Thr Pro Pro Met Leu Asn Val Pro Glu His Phe Arg Arg 
145                 150                 155                 160 


Pro Phe Asn Ile Gly Leu Tyr Lys Gly Thr Val Glu Leu Thr Met Thr 
                165                 170                 175     


Ile Tyr Asp Asp Glu Ser Leu Glu Ala Ala Pro Met Ile Trp Asp His 
            180                 185                 190         


Phe Asn Ser Ser Lys Phe Ser Asp Phe Arg Asp Lys Ala Leu Met Phe 
        195                 200                 205             


Gly Leu Ile Val Glu Lys Lys Ala Ser Gly Ala Trp Val Leu Asp Ser 
    210                 215                 220                 


Val Ser His Phe Lys 
225                 


<210>  4
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Mutant G21E/L111A/M51R

<400>  4

Met Ser Ser Leu Lys Lys Ile Leu Gly Leu Lys Gly Lys Gly Lys Lys 
1               5                   10                  15      


Ser Lys Lys Leu Glu Ile Ala Pro Pro Pro Tyr Glu Glu Asp Thr Asn 
            20                  25                  30          


Met Glu Tyr Ala Pro Ser Ala Pro Ile Asp Lys Ser Tyr Phe Gly Val 
        35                  40                  45              


Asp Glu Arg Asp Thr His Asp Pro His Gln Leu Arg Tyr Glu Lys Phe 
    50                  55                  60                  


Phe Phe Thr Val Lys Met Thr Val Arg Ser Asn Arg Pro Phe Arg Thr 
65                  70                  75                  80  


Tyr Ser Asp Val Ala Ala Ala Val Ser His Trp Asp His Met Tyr Ile 
                85                  90                  95      


Gly Met Ala Gly Lys Arg Pro Phe Tyr Lys Ile Leu Ala Phe Ala Gly 
            100                 105                 110         


Ser Ser Asn Leu Lys Ala Thr Pro Ala Val Leu Ala Asp Gln Gly Gln 
        115                 120                 125             


Pro Glu Tyr His Ala His Cys Glu Gly Arg Ala Tyr Leu Pro His Arg 
    130                 135                 140                 


Met Gly Lys Thr Pro Pro Met Leu Asn Val Pro Glu His Phe Arg Arg 
145                 150                 155                 160 


Pro Phe Asn Ile Gly Leu Tyr Lys Gly Thr Val Glu Leu Thr Met Thr 
                165                 170                 175     


Ile Tyr Asp Asp Glu Ser Leu Glu Ala Ala Pro Met Ile Trp Asp His 
            180                 185                 190         


Phe Asn Ser Ser Lys Phe Ser Asp Phe Arg Asp Lys Ala Leu Met Phe 
        195                 200                 205             


Gly Leu Ile Val Glu Lys Lys Ala Ser Gly Ala Trp Val Leu Asp Ser 
    210                 215                 220                 


Val Ser His Phe Lys 
225                 


<210>  5
<211>  686
<212>  DNA
<213>  Vesicular stomatitis New Jersey virus


<220>
<221>  SITE
<222>  (1)..(686)
<223>  VSV New Jersey serotype Wild Type

<400>  5
atgagttcct tcaaaaagat tctgggattt tcttcaaaaa gtcacaagaa atcaaagaaa       60

ctaggcttgc cacctcctta tgaggaatca agtcctatgg agattcaacc atctgcccca      120

ttatcaaatg acttcttcgg aatggaggat atggatttat atgataagga ctccttgaga      180

tatgagaagt tccgctttat gttgaagatg actgttagag ctaacaagcc cttcagatcg      240

tatgatgatg tcaccgcagc ggtatcacaa tgggataatt catacattgg aatggttgga      300

aagcgtcctt tctacaagat aattgctctg attggctcca gtcatctgca agcaactcca      360

gctgtgttgg cagacttaaa tcaaccagag tattatgcca cactaacagg tcgttgtttt      420

cttcctcacc gactcggatt gatcccaccg atgtttaatg tgtccgaaac tttcagaaaa      480

ccattcaata ttgggatata caaagggact ctcgacttca cctttacagt ttcagatgat      540

gagtctaatg aaaaagtccc tcatgtttgg gaatacatga acccaaaata tcaatctcag      600

atccaaaaag aagggcttaa attcggattg attttaagca agaaagcaac gggaacttgg      660

gtgttagacc aattgagtcc gtttaa                                           686


<210>  6
<211>  686
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G22E/M48R/M51R

<400>  6
atgagttcct tcaaaaagat tctgggattt tcttcaaaaa gtcacaagaa atcaaagaaa       60

ctagaattgc cacctcctta tgaggaatca agtcctatgg agattcaacc atctgcccca      120

ttatcaaatg acttcttcgg acgagaggat cgagatttat atgataagga ctccttgaga      180

tatgagaagt tccgctttat gttgaagatg actgttagag ctaacaagcc cttcagatcg      240

tatgatgatg tcaccgcagc ggtatcacaa tgggataatt catacattgg aatggttgga      300

aagcgtcctt tctacaagat aattgctctg attggctcca gtcatctgca agcaactcca      360

gctgtgttgg cagacttaaa tcaaccagag tattatgcca cactaacagg tcgttgtttt      420

cttcctcacc gactcggatt gatcccaccg atgtttaatg tgtccgaaac tttcagaaaa      480

ccattcaata ttgggatata caaagggact ctcgacttca cctttacagt ttcagatgat      540

gagtctaatg aaaaagtccc tcatgtttgg gaatacatga acccaaaata tcaatctcag      600

atccaaaaag aagggcttaa attcggattg attttaagca agaaagcaac gggaacttgg      660

gtgttagacc aattgagtcc gtttaa                                           686


<210>  7
<211>  686
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G22E/L110A/M48R/M51R

<400>  7
atgagttcct tcaaaaagat tctgggattt tcttcaaaaa gtcacaagaa atcaaagaaa       60

ctagaattgc cacctcctta tgaggaatca agtcctatgg agattcaacc atctgcccca      120

ttatcaaatg acttcttcgg acgagaggat cgagatttat atgataagga ctccttgaga      180

tatgagaagt tccgctttat gttgaagatg actgttagag ctaacaagcc cttcagatcg      240

tatgatgatg tcaccgcagc ggtatcacaa tgggataatt catacattgg aatggttgga      300

aagcgtcctt tctacaagat aattgctgca attggctcca gtcatctgca agcaactcca      360

gctgtgttgg cagacttaaa tcaaccagag tattatgcca cactaacagg tcgttgtttt      420

cttcctcacc gactcggatt gatcccaccg atgtttaatg tgtccgaaac tttcagaaaa      480

ccattcaata ttgggatata caaagggact ctcgacttca cctttacagt ttcagatgat      540

gagtctaatg aaaaagtccc tcatgtttgg gaatacatga acccaaaata tcaatctcag      600

atccaaaaag aagggcttaa attcggattg attttaagca agaaagcaac gggaacttgg      660

gtgttagacc aattgagtcc gtttaa                                           686


<210>  8
<211>  229
<212>  PRT
<213>  Vesicular stomatitis New Jersey virus


<220>
<221>  SITE
<222>  (1)..(229)
<223>  VSV New Jersey serotype Wild Type

<400>  8

Met Ser Ser Phe Lys Lys Ile Leu Gly Phe Ser Ser Lys Ser His Lys 
1               5                   10                  15      


Lys Ser Lys Lys Leu Gly Leu Pro Pro Pro Tyr Glu Glu Ser Ser Pro 
            20                  25                  30          


Met Glu Ile Gln Pro Ser Ala Pro Leu Ser Asn Asp Phe Phe Gly Met 
        35                  40                  45              


Glu Asp Met Asp Leu Tyr Asp Lys Asp Ser Leu Arg Tyr Glu Lys Phe 
    50                  55                  60                  


Arg Phe Met Leu Lys Met Thr Val Arg Ala Asn Lys Pro Phe Arg Ser 
65                  70                  75                  80  


Tyr Asp Asp Val Thr Ala Ala Val Ser Gln Trp Asp Asn Ser Tyr Ile 
                85                  90                  95      


Gly Met Val Gly Lys Arg Pro Phe Tyr Lys Ile Ile Ala Leu Ile Gly 
            100                 105                 110         


Ser Ser His Leu Gln Ala Thr Pro Ala Val Leu Ala Asp Leu Asn Gln 
        115                 120                 125             


Pro Glu Tyr Tyr Ala Thr Leu Thr Gly Arg Cys Phe Leu Pro His Arg 
    130                 135                 140                 


Leu Gly Leu Ile Pro Pro Met Phe Asn Val Ser Glu Thr Phe Arg Lys 
145                 150                 155                 160 


Pro Phe Asn Ile Gly Ile Tyr Lys Gly Thr Leu Asp Phe Thr Phe Thr 
                165                 170                 175     


Val Ser Asp Asp Glu Ser Asn Glu Lys Val Pro His Val Trp Glu Tyr 
            180                 185                 190         


Met Asn Pro Lys Tyr Gln Ser Gln Ile Gln Lys Glu Gly Leu Lys Phe 
        195                 200                 205             


Gly Leu Ile Leu Ser Lys Lys Ala Thr Gly Thr Trp Val Leu Asp Gln 
    210                 215                 220                 


Leu Ser Pro Phe Lys 
225                 


<210>  9
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: G22E/M48R/M51R

<400>  9

Met Ser Ser Phe Lys Lys Ile Leu Gly Phe Ser Ser Lys Ser His Lys 
1               5                   10                  15      


Lys Ser Lys Lys Leu Glu Leu Pro Pro Pro Tyr Glu Glu Ser Ser Pro 
            20                  25                  30          


Met Glu Ile Gln Pro Ser Ala Pro Leu Ser Asn Asp Phe Phe Gly Arg 
        35                  40                  45              


Glu Asp Arg Asp Leu Tyr Asp Lys Asp Ser Leu Arg Tyr Glu Lys Phe 
    50                  55                  60                  


Arg Phe Met Leu Lys Met Thr Val Arg Ala Asn Lys Pro Phe Arg Ser 
65                  70                  75                  80  


Tyr Asp Asp Val Thr Ala Ala Val Ser Gln Trp Asp Asn Ser Tyr Ile 
                85                  90                  95      


Gly Met Val Gly Lys Arg Pro Phe Tyr Lys Ile Ile Ala Leu Ile Gly 
            100                 105                 110         


Ser Ser His Leu Gln Ala Thr Pro Ala Val Leu Ala Asp Leu Asn Gln 
        115                 120                 125             


Pro Glu Tyr Tyr Ala Thr Leu Thr Gly Arg Cys Phe Leu Pro His Arg 
    130                 135                 140                 


Leu Gly Leu Ile Pro Pro Met Phe Asn Val Ser Glu Thr Phe Arg Lys 
145                 150                 155                 160 


Pro Phe Asn Ile Gly Ile Tyr Lys Gly Thr Leu Asp Phe Thr Phe Thr 
                165                 170                 175     


Val Ser Asp Asp Glu Ser Asn Glu Lys Val Pro His Val Trp Glu Tyr 
            180                 185                 190         


Met Asn Pro Lys Tyr Gln Ser Gln Ile Gln Lys Glu Gly Leu Lys Phe 
        195                 200                 205             


Gly Leu Ile Leu Ser Lys Lys Ala Thr Gly Thr Trp Val Leu Asp Gln 
    210                 215                 220                 


Leu Ser Pro Phe Lys 
225                 


<210>  10
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: G22E/L110A/M48R/M51R

<400>  10

Met Ser Ser Phe Lys Lys Ile Leu Gly Phe Ser Ser Lys Ser His Lys 
1               5                   10                  15      


Lys Ser Lys Lys Leu Glu Leu Pro Pro Pro Tyr Glu Glu Ser Ser Pro 
            20                  25                  30          


Met Glu Ile Gln Pro Ser Ala Pro Leu Ser Asn Asp Phe Phe Gly Arg 
        35                  40                  45              


Glu Asp Arg Asp Leu Tyr Asp Lys Asp Ser Leu Arg Tyr Glu Lys Phe 
    50                  55                  60                  


Arg Phe Met Leu Lys Met Thr Val Arg Ala Asn Lys Pro Phe Arg Ser 
65                  70                  75                  80  


Tyr Asp Asp Val Thr Ala Ala Val Ser Gln Trp Asp Asn Ser Tyr Ile 
                85                  90                  95      


Gly Met Val Gly Lys Arg Pro Phe Tyr Lys Ile Ile Ala Ala Ile Gly 
            100                 105                 110         


Ser Ser His Leu Gln Ala Thr Pro Ala Val Leu Ala Asp Leu Asn Gln 
        115                 120                 125             


Pro Glu Tyr Tyr Ala Thr Leu Thr Gly Arg Cys Phe Leu Pro His Arg 
    130                 135                 140                 


Leu Gly Leu Ile Pro Pro Met Phe Asn Val Ser Glu Thr Phe Arg Lys 
145                 150                 155                 160 


Pro Phe Asn Ile Gly Ile Tyr Lys Gly Thr Leu Asp Phe Thr Phe Thr 
                165                 170                 175     


Val Ser Asp Asp Glu Ser Asn Glu Lys Val Pro His Val Trp Glu Tyr 
            180                 185                 190         


Met Asn Pro Lys Tyr Gln Ser Gln Ile Gln Lys Glu Gly Leu Lys Phe 
        195                 200                 205             


Gly Leu Ile Leu Ser Lys Lys Ala Thr Gly Thr Trp Val Leu Asp Gln 
    210                 215                 220                 


Leu Ser Pro Phe Lys 
225                 


<210>  11
<211>  21
<212>  PRT
<213>  Apis mellifera


<220>
<221>  SITE
<222>  (1)..(21)
<223>  Honeybee melittin signal peptide

<400>  11

Met Lys Phe Leu Val Asn Val Ala Leu Val Phe Met Val Val Tyr Ile 
1               5                   10                  15      


Ser Tyr Ile Tyr Ala 
            20      


<210>  12
<211>  49
<212>  PRT
<213>  Unknown

<220>
<223>  Synthetic: VSV G protein transmembrane domain and cytoplasmic 
       tail

<400>  12

Ser Ser Ile Ala Ser Phe Phe Phe Ile Ile Gly Leu Ile Ile Gly Leu 
1               5                   10                  15      


Phe Leu Val Leu Arg Val Gly Ile Tyr Leu Cys Ile Lys Leu Lys His 
            20                  25                  30          


Thr Lys Lys Arg Gln Ile Tyr Thr Asp Ile Glu Met Asn Arg Leu Gly 
        35                  40                  45              


Lys 
    


<210>  13
<211>  25
<212>  DNA
<213>  Unknown

<220>
<223>  Synthetic: VSV intergenic junctions

<400>  13
catatgaaaa aaactaacag atatc                                             25


