                         SEQUENCE LISTING

<110>  Autolus Limited
 
<120>  Generation of T-cells by direct reprogramming from fibroblasts 
       and MSC

<130>  |P10787GBWO|

<150>  GB2103101.8
<151>  2021-03-05

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  508
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human cytomegalovirus promoter such as the long CMV promoter, or 
       cytomegalovirus (CMV) immediate-early enhancer - promoter

<400>  1
cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt       60

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      120

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      180

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      240

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      300

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      360

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      420

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      480

acggtgggag gtctatataa gcagagct                                         508


<210>  2
<211>  1189
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human EF1alpha promoter

<400>  2
cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt       60

tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg      120

aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa      180

gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa      240

gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt      300

gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga      360

agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt      420

gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt      480

ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt      540

tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt      600

tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg      660

ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct      720

ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg      780

tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca      840

aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg      900

gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg      960

cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt     1020

tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac     1080

ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag     1140

cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgag                 1189


<210>  3
<211>  516
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human PGK promoter

<400>  3
ccacggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct       60

gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca      120

cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc      180

ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac      240

aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg      300

cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga      360

gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt      420

tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct      480

ccctcgttga ccgaatcacc gacctctctc cccagg                                516


<210>  4
<211>  409
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spleen focus forming virus (SFFV) promoter

<400>  4
gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca       60

agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt      120

tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc      180

caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga      240

tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc      300

agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac      360

aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccgggg                  409


<210>  5
<211>  4792
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SFFV- TBET-ETS1-TCF7 expression cassette

<400>  5
gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca       60

agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt      120

tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc      180

caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga      240

tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc      300

agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac      360

aacccctcac tcggcgcgcc agtcctccga cagactgagt cggccggtcg aatcaagctt      420

atcgataccg tcgacggatc cggatccgat gggcatcgtg gagccgggtt gcggagacat      480

gctgacgggc accgagccga tgccggggag cgacgagggc cgggcgcctg gcgccgaccc      540

gcagcaccgc tacttctacc cggagccggg cgcgcaggac gcggacgagc gtcgcggggg      600

cggcagcctg gggtctccct acccgggggg cgccttggtg cccgccccgc cgagccgctt      660

ccttggagcc tacgcctacc cgccgcgacc ccaggcggcc ggcttccccg gcgcgggcga      720

gtccttcccg ccgcccgcgg acgccgaggg ctaccagccg ggcgagggct acgccgcccc      780

ggacccgcgc gccgggctct acccggggcc gcgtgaggac tacgcgctac ccgcgggact      840

ggaggtgtcg gggaaactga gggtcgcgct caacaaccac ctgttgtggt ccaagtttaa      900

tcagcaccag acagagatga tcatcaccaa gcagggacgg cggatgttcc cattcctgtc      960

atttactgtg gccgggctgg agcccaccag ccactacagg atgtttgtgg acgtggtctt     1020

ggtggaccag caccactggc ggtaccagag cggcaagtgg gtgcagtgtg gaaaggccga     1080

gggcagcatg ccaggaaacc gcctgtacgt ccacccggac tcccccaaca caggagcgca     1140

ctggatgcgc caggaagttt catttgggaa actaaagctc acaaacaaca agggggcgtc     1200

caacaatgtg acccagatga ttgtgctcca gtccctccat aagtaccagc cccggctgca     1260

tatcgttgag gtgaacgacg gagagccaga ggcagcctgc aacgcttcca acacgcatat     1320

ctttactttc caagaaaccc agttcattgc cgtgactgcc taccagaatg ccgagattac     1380

tcagctgaaa attgataata acccctttgc caaaggattc cgggagaact ttgagtccat     1440

gtacacatct gttgacacca gcatcccctc cccgcctgga cccaactgtc aattccttgg     1500

gggagatcac tactctcctc tcctacccaa ccagtatcct gttcccagcc gcttctaccc     1560

cgaccttcct ggccaggcga aggatgtggt tccccaggct tactggctgg gggccccccg     1620

ggaccacagc tatgaggctg agtttcgagc agtcagcatg aagcctgcat tcttgccctc     1680

tgcccctggg cccaccatgt cctactaccg aggccaggag gtcctggcac ctggagctgg     1740

ctggcctgtg gcaccccagt accctcccaa gatgggcccg gccagctggt tccgccctat     1800

gcggactctg cccatggaac ccggccctgg aggctcagag ggacggggac cagaggacca     1860

gggtcccccc ttggtgtgga ctgagattgc ccccatccgg ccggaatcca gtgattcagg     1920

actgggcgaa ggagactcta agaggaggcg cgtgtccccc tatccttcca gtggtgacag     1980

ctcctcccct gctggggccc cttctccttt tgataaggaa gctgaaggac agttttataa     2040

ctattttccc aacggcagcg gcgccacaaa cttctctctg ctaaagcaag caggtgatgt     2100

tgaagaaaac cccgggccta tgagctactt tgtggattct gctgggagca gccccgtccc     2160

ttactcagcg cctcgtcctg cagtggtgag gcaaggacct agcaacactt atgaagatcc     2220

tcgaatgaac tgtggtttcc agtccaatta tcaccagcaa agaccttgct accccttttg     2280

ggatgagatg gcaactcagg aagttcctac tggtcttgaa cactgtgtct cagatatgga     2340

atgtgcagat gtcccactat taactccaag cagcaaagaa atgatgtctc aagcattaaa     2400

agctactttc agtggtttca ctaaagaaca gcaacgactg gggatcccaa aagacccccg     2460

gcagtggaca gaaacccatg ttcgggactg ggtgatgtgg gctgtgaatg aattcagcct     2520

gaaaggtgta gacttccaga agttctgtat gaatggagca gccctctgcg ccctgggtaa     2580

agactgcttt ctcgagctgg ccccagactt tgttggggac atcttatggg aacatctaga     2640

gatcctgcag aaagaggatg tgaaaccata tcaagttaat ggagtcaacc cagcctatcc     2700

agaatcccgc tatacctcgg attacttcat tagctatggt attgagcatg cccagtgtgt     2760

tccaccatcg gagttctcag agcccagctt catcacagag tcctatcaga cgctccatcc     2820

catcagctcg gaagagctcc tctccctcaa gtatgagaat gactacccct cggtcattct     2880

ccgagaccct ctccagacag acaccttgca gaatgactac tttgctatca aacaagaagt     2940

cgtcacccca gacaacatgt gcatggggag gaccagtcgt ggtaaactcg ggggccagga     3000

ctcttttgaa agcatagaga gctacgatag ttgtgatcgc ctcacccagt cctggagcag     3060

ccagtcatct ttcaacagcc tgcagcgtgt tccctcctat gacagcttcg actcagagga     3120

ctatccggct gccctgccca accacaagcc caagggcacc ttcaaggact atgtgcggga     3180

ccgtgctgac ctcaataagg acaagcctgt cattcctgct gctgccctag ctggctacac     3240

aggcagtgga ccaatccagc tatggcagtt tcttctggaa ttactcactg ataaatcctg     3300

tcagtctttt atcagctgga caggagatgg ctgggaattc aaactttctg acccagatga     3360

ggtggccagg agatggggaa agaggaaaaa caaacctaag atgaattatg agaaactgag     3420

ccgtggccta cgctactatt acgacaaaaa catcatccac aagacagcgg ggaaacgcta     3480

cgtgtaccgc tttgtgtgtg acctgcagag cctgctgggg tacacccctg aggagctgca     3540

cgccatgctg gacgtcaagc cagatgccga cgagggctcc ggcgagggca ggggaagtct     3600

tctaacatgc ggggacgtgg aggaaaatcc cggcccaatg ccgcagctgg actccggcgg     3660

gggcggcgcg ggcggcggcg acgacctcgg cgcgccggac gagctgctgg ccttccagga     3720

tgaaggcgag gagcaggacg acaagagccg cgacagcgcc gccggtcccg agcgcgacct     3780

ggccgagctc aagtcgtcgc tcgtgaacga gtccgagggc gcggccggcg gcgcagggat     3840

cccgggggtc ccgggggccg gcgccggggc ccgcggcgag gccgaggctc tcgggcggga     3900

acacgctgcg cagagactct tcccggacaa acttccagag cccctggagg acggcctgaa     3960

ggccccggag tgcaccagcg gcatgtacaa agagaccgtc tactccgcct tcaatctgct     4020

catgcattac ccacccccct cgggagcagg gcagcacccc cagccgcagc ccccgctgca     4080

caaggccaat cagccccccc acggtgtccc ccaactctct ctctacgaac atttcaacag     4140

cccacatccc acccctgcac ctgcggacat cagccagaag caagttcaca ggcctctgca     4200

gacccctgac ctctctggct tctactccct gacctcaggc agcatggggc agctccccca     4260

cactgtgagc tggttcaccc acccatcctt gatgctaggt tctggtgtac ctggtcaccc     4320

agcagccatc ccccacccgg ccattgtgcc cccctcaggg aagcaggagc tgcagccctt     4380

cgaccgcaac ctgaagacac aagcagagtc caaggcagag aaggaggcca agaagccaac     4440

catcaagaag cccctcaatg ccttcatgct gtacatgaag gagatgagag ccaaggtcat     4500

tgcagagtgc acacttaagg agagcgctgc catcaaccag atcctgggcc gcaggtggca     4560

cgcgctgtcg cgagaagagc aggccaagta ctatgagctg gcccgcaagg agaggcagct     4620

gcacatgcag ctatacccag gctggtcagc gcgggacaac tacgggaaga agaagaggcg     4680

gtcgagggaa aagcaccaag aatccaccac aggaggaaaa agaaatgcat tcggtactta     4740

cccggagaag gccgctgccc cagccccgtt ccttccgatg acagtgctct ag             4792


<210>  6
<211>  4802
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fuw-TetO- TBET-ETS1-TCF7  expression cassette

<400>  6
tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag       60

aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt      120

taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg      180

atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt      240

cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt      300

cgagtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga      360

tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca      420

gcctccgcgg ccccgaattc accggttcta gagctagcat gggcatcgtg gagccgggtt      480

gcggagacat gctgacgggc accgagccga tgccggggag cgacgagggc cgggcgcctg      540

gcgccgaccc gcagcaccgc tacttctacc cggagccggg cgcgcaggac gcggacgagc      600

gtcgcggggg cggcagcctg gggtctccct acccgggggg cgccttggtg cccgccccgc      660

cgagccgctt ccttggagcc tacgcctacc cgccgcgacc ccaggcggcc ggcttccccg      720

gcgcgggcga gtccttcccg ccgcccgcgg acgccgaggg ctaccagccg ggcgagggct      780

acgccgcccc ggacccgcgc gccgggctct acccggggcc gcgtgaggac tacgcgctac      840

ccgcgggact ggaggtgtcg gggaaactga gggtcgcgct caacaaccac ctgttgtggt      900

ccaagtttaa tcagcaccag acagagatga tcatcaccaa gcagggacgg cggatgttcc      960

cattcctgtc atttactgtg gccgggctgg agcccaccag ccactacagg atgtttgtgg     1020

acgtggtctt ggtggaccag caccactggc ggtaccagag cggcaagtgg gtgcagtgtg     1080

gaaaggccga gggcagcatg ccaggaaacc gcctgtacgt ccacccggac tcccccaaca     1140

caggagcgca ctggatgcgc caggaagttt catttgggaa actaaagctc acaaacaaca     1200

agggggcgtc caacaatgtg acccagatga ttgtgctcca gtccctccat aagtaccagc     1260

cccggctgca tatcgttgag gtgaacgacg gagagccaga ggcagcctgc aacgcttcca     1320

acacgcatat ctttactttc caagaaaccc agttcattgc cgtgactgcc taccagaatg     1380

ccgagattac tcagctgaaa attgataata acccctttgc caaaggattc cgggagaact     1440

ttgagtccat gtacacatct gttgacacca gcatcccctc cccgcctgga cccaactgtc     1500

aattccttgg gggagatcac tactctcctc tcctacccaa ccagtatcct gttcccagcc     1560

gcttctaccc cgaccttcct ggccaggcga aggatgtggt tccccaggct tactggctgg     1620

gggccccccg ggaccacagc tatgaggctg agtttcgagc agtcagcatg aagcctgcat     1680

tcttgccctc tgcccctggg cccaccatgt cctactaccg aggccaggag gtcctggcac     1740

ctggagctgg ctggcctgtg gcaccccagt accctcccaa gatgggcccg gccagctggt     1800

tccgccctat gcggactctg cccatggaac ccggccctgg aggctcagag ggacggggac     1860

cagaggacca gggtcccccc ttggtgtgga ctgagattgc ccccatccgg ccggaatcca     1920

gtgattcagg actgggcgaa ggagactcta agaggaggcg cgtgtccccc tatccttcca     1980

gtggtgacag ctcctcccct gctggggccc cttctccttt tgataaggaa gctgaaggac     2040

agttttataa ctattttccc aacggcagcg gcgccacaaa cttctctctg ctaaagcaag     2100

caggtgatgt tgaagaaaac cccgggccta tgagctactt tgtggattct gctgggagca     2160

gccccgtccc ttactcagcg cctcgtcctg cagtggtgag gcaaggacct agcaacactt     2220

atgaagatcc tcgaatgaac tgtggtttcc agtccaatta tcaccagcaa agaccttgct     2280

accccttttg ggatgagatg gcaactcagg aagttcctac tggtcttgaa cactgtgtct     2340

cagatatgga atgtgcagat gtcccactat taactccaag cagcaaagaa atgatgtctc     2400

aagcattaaa agctactttc agtggtttca ctaaagaaca gcaacgactg gggatcccaa     2460

aagacccccg gcagtggaca gaaacccatg ttcgggactg ggtgatgtgg gctgtgaatg     2520

aattcagcct gaaaggtgta gacttccaga agttctgtat gaatggagca gccctctgcg     2580

ccctgggtaa agactgcttt ctcgagctgg ccccagactt tgttggggac atcttatggg     2640

aacatctaga gatcctgcag aaagaggatg tgaaaccata tcaagttaat ggagtcaacc     2700

cagcctatcc agaatcccgc tatacctcgg attacttcat tagctatggt attgagcatg     2760

cccagtgtgt tccaccatcg gagttctcag agcccagctt catcacagag tcctatcaga     2820

cgctccatcc catcagctcg gaagagctcc tctccctcaa gtatgagaat gactacccct     2880

cggtcattct ccgagaccct ctccagacag acaccttgca gaatgactac tttgctatca     2940

aacaagaagt cgtcacccca gacaacatgt gcatggggag gaccagtcgt ggtaaactcg     3000

ggggccagga ctcttttgaa agcatagaga gctacgatag ttgtgatcgc ctcacccagt     3060

cctggagcag ccagtcatct ttcaacagcc tgcagcgtgt tccctcctat gacagcttcg     3120

actcagagga ctatccggct gccctgccca accacaagcc caagggcacc ttcaaggact     3180

atgtgcggga ccgtgctgac ctcaataagg acaagcctgt cattcctgct gctgccctag     3240

ctggctacac aggcagtgga ccaatccagc tatggcagtt tcttctggaa ttactcactg     3300

ataaatcctg tcagtctttt atcagctgga caggagatgg ctgggaattc aaactttctg     3360

acccagatga ggtggccagg agatggggaa agaggaaaaa caaacctaag atgaattatg     3420

agaaactgag ccgtggccta cgctactatt acgacaaaaa catcatccac aagacagcgg     3480

ggaaacgcta cgtgtaccgc tttgtgtgtg acctgcagag cctgctgggg tacacccctg     3540

aggagctgca cgccatgctg gacgtcaagc cagatgccga cgagggctcc ggcgagggca     3600

ggggaagtct tctaacatgc ggggacgtgg aggaaaatcc cggcccaatg ccgcagctgg     3660

actccggcgg gggcggcgcg ggcggcggcg acgacctcgg cgcgccggac gagctgctgg     3720

ccttccagga tgaaggcgag gagcaggacg acaagagccg cgacagcgcc gccggtcccg     3780

agcgcgacct ggccgagctc aagtcgtcgc tcgtgaacga gtccgagggc gcggccggcg     3840

gcgcagggat cccgggggtc ccgggggccg gcgccggggc ccgcggcgag gccgaggctc     3900

tcgggcggga acacgctgcg cagagactct tcccggacaa acttccagag cccctggagg     3960

acggcctgaa ggccccggag tgcaccagcg gcatgtacaa agagaccgtc tactccgcct     4020

tcaatctgct catgcattac ccacccccct cgggagcagg gcagcacccc cagccgcagc     4080

ccccgctgca caaggccaat cagccccccc acggtgtccc ccaactctct ctctacgaac     4140

atttcaacag cccacatccc acccctgcac ctgcggacat cagccagaag caagttcaca     4200

ggcctctgca gacccctgac ctctctggct tctactccct gacctcaggc agcatggggc     4260

agctccccca cactgtgagc tggttcaccc acccatcctt gatgctaggt tctggtgtac     4320

ctggtcaccc agcagccatc ccccacccgg ccattgtgcc cccctcaggg aagcaggagc     4380

tgcagccctt cgaccgcaac ctgaagacac aagcagagtc caaggcagag aaggaggcca     4440

agaagccaac catcaagaag cccctcaatg ccttcatgct gtacatgaag gagatgagag     4500

ccaaggtcat tgcagagtgc acacttaagg agagcgctgc catcaaccag atcctgggcc     4560

gcaggtggca cgcgctgtcg cgagaagagc aggccaagta ctatgagctg gcccgcaagg     4620

agaggcagct gcacatgcag ctatacccag gctggtcagc gcgggacaac tacgggaaga     4680

agaagaggcg gtcgagggaa aagcaccaag aatccaccac aggaggaaaa agaaatgcat     4740

tcggtactta cccggagaag gccgctgccc cagccccgtt ccttccgatg acagtgctct     4800

ag                                                                    4802


<210>  7
<211>  4344
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  exemplary mRNA encoding TBET-ETS1-TCF7

<400>  7
augggcaucg uggagccggg uugcggagac augcugacgg gcaccgagcc gaugccgggg       60

agcgacgagg gccgggcgcc uggcgccgac ccgcagcacc gcuacuucua cccggagccg      120

ggcgcgcagg acgcggacga gcgucgcggg ggcggcagcc uggggucucc cuacccgggg      180

ggcgccuugg ugcccgcccc gccgagccgc uuccuuggag ccuacgccua cccgccgcga      240

ccccaggcgg ccggcuuccc cggcgcgggc gaguccuucc cgccgcccgc ggacgccgag      300

ggcuaccagc cgggcgaggg cuacgccgcc ccggacccgc gcgccgggcu cuacccgggg      360

ccgcgugagg acuacgcgcu acccgcggga cuggaggugu cggggaaacu gagggucgcg      420

cucaacaacc accuguugug guccaaguuu aaucagcacc agacagagau gaucaucacc      480

aagcagggac ggcggauguu cccauuccug ucauuuacug uggccgggcu ggagcccacc      540

agccacuaca ggauguuugu ggacgugguc uugguggacc agcaccacug gcgguaccag      600

agcggcaagu gggugcagug uggaaaggcc gagggcagca ugccaggaaa ccgccuguac      660

guccacccgg acucccccaa cacaggagcg cacuggaugc gccaggaagu uucauuuggg      720

aaacuaaagc ucacaaacaa caagggggcg uccaacaaug ugacccagau gauugugcuc      780

cagucccucc auaaguacca gccccggcug cauaucguug aggugaacga cggagagcca      840

gaggcagccu gcaacgcuuc caacacgcau aucuuuacuu uccaagaaac ccaguucauu      900

gccgugacug ccuaccagaa ugccgagauu acucagcuga aaauugauaa uaaccccuuu      960

gccaaaggau uccgggagaa cuuugagucc auguacacau cuguugacac cagcaucccc     1020

uccccgccug gacccaacug ucaauuccuu gggggagauc acuacucucc ucuccuaccc     1080

aaccaguauc cuguucccag ccgcuucuac cccgaccuuc cuggccaggc gaaggaugug     1140

guuccccagg cuuacuggcu gggggccccc cgggaccaca gcuaugaggc ugaguuucga     1200

gcagucagca ugaagccugc auucuugccc ucugccccug ggcccaccau guccuacuac     1260

cgaggccagg agguccuggc accuggagcu ggcuggccug uggcacccca guacccuccc     1320

aagaugggcc cggccagcug guuccgcccu augcggacuc ugcccaugga acccggcccu     1380

ggaggcucag agggacgggg accagaggac cagggucccc ccuuggugug gacugagauu     1440

gcccccaucc ggccggaauc cagugauuca ggacugggcg aaggagacuc uaagaggagg     1500

cgcguguccc ccuauccuuc caguggugac agcuccuccc cugcuggggc cccuucuccu     1560

uuugauaagg aagcugaagg acaguuuuau aacuauuuuc ccaacggcag cggcgccaca     1620

aacuucucuc ugcuaaagca agcaggugau guugaagaaa accccgggcc uaugagcuac     1680

uuuguggauu cugcugggag cagccccguc ccuuacucag cgccucgucc ugcaguggug     1740

aggcaaggac cuagcaacac uuaugaagau ccucgaauga acugugguuu ccaguccaau     1800

uaucaccagc aaagaccuug cuaccccuuu ugggaugaga uggcaacuca ggaaguuccu     1860

acuggucuug aacacugugu cucagauaug gaaugugcag augucccacu auuaacucca     1920

agcagcaaag aaaugauguc ucaagcauua aaagcuacuu ucagugguuu cacuaaagaa     1980

cagcaacgac uggggauccc aaaagacccc cggcagugga cagaaaccca uguucgggac     2040

ugggugaugu gggcugugaa ugaauucagc cugaaaggug uagacuucca gaaguucugu     2100

augaauggag cagcccucug cgcccugggu aaagacugcu uucucgagcu ggccccagac     2160

uuuguugggg acaucuuaug ggaacaucua gagauccugc agaaagagga ugugaaacca     2220

uaucaaguua auggagucaa cccagccuau ccagaauccc gcuauaccuc ggauuacuuc     2280

auuagcuaug guauugagca ugcccagugu guuccaccau cggaguucuc agagcccagc     2340

uucaucacag aguccuauca gacgcuccau cccaucagcu cggaagagcu ccucucccuc     2400

aaguaugaga augacuaccc cucggucauu cuccgagacc cucuccagac agacaccuug     2460

cagaaugacu acuuugcuau caaacaagaa gucgucaccc cagacaacau gugcaugggg     2520

aggaccaguc gugguaaacu cgggggccag gacucuuuug aaagcauaga gagcuacgau     2580

aguugugauc gccucaccca guccuggagc agccagucau cuuucaacag ccugcagcgu     2640

guucccuccu augacagcuu cgacucagag gacuauccgg cugcccugcc caaccacaag     2700

cccaagggca ccuucaagga cuaugugcgg gaccgugcug accucaauaa ggacaagccu     2760

gucauuccug cugcugcccu agcuggcuac acaggcagug gaccaaucca gcuauggcag     2820

uuucuucugg aauuacucac ugauaaaucc ugucagucuu uuaucagcug gacaggagau     2880

ggcugggaau ucaaacuuuc ugacccagau gagguggcca ggagaugggg aaagaggaaa     2940

aacaaaccua agaugaauua ugagaaacug agccguggcc uacgcuacua uuacgacaaa     3000

aacaucaucc acaagacagc ggggaaacgc uacguguacc gcuuugugug ugaccugcag     3060

agccugcugg gguacacccc ugaggagcug cacgccaugc uggacgucaa gccagaugcc     3120

gacgagggcu ccggcgaggg caggggaagu cuucuaacau gcggggacgu ggaggaaaau     3180

cccggcccaa ugccgcagcu ggacuccggc gggggcggcg cgggcggcgg cgacgaccuc     3240

ggcgcgccgg acgagcugcu ggccuuccag gaugaaggcg aggagcagga cgacaagagc     3300

cgcgacagcg ccgccggucc cgagcgcgac cuggccgagc ucaagucguc gcucgugaac     3360

gaguccgagg gcgcggccgg cggcgcaggg aucccggggg ucccgggggc cggcgccggg     3420

gcccgcggcg aggccgaggc ucucgggcgg gaacacgcug cgcagagacu cuucccggac     3480

aaacuuccag agccccugga ggacggccug aaggccccgg agugcaccag cggcauguac     3540

aaagagaccg ucuacuccgc cuucaaucug cucaugcauu acccaccccc cucgggagca     3600

gggcagcacc cccagccgca gcccccgcug cacaaggcca aucagccccc ccacgguguc     3660

ccccaacucu cucucuacga acauuucaac agcccacauc ccaccccugc accugcggac     3720

aucagccaga agcaaguuca caggccucug cagaccccug accucucugg cuucuacucc     3780

cugaccucag gcagcauggg gcagcucccc cacacuguga gcugguucac ccacccaucc     3840

uugaugcuag guucuggugu accuggucac ccagcagcca ucccccaccc ggccauugug     3900

ccccccucag ggaagcagga gcugcagccc uucgaccgca accugaagac acaagcagag     3960

uccaaggcag agaaggaggc caagaagcca accaucaaga agccccucaa ugccuucaug     4020

cuguacauga aggagaugag agccaagguc auugcagagu gcacacuuaa ggagagcgcu     4080

gccaucaacc agauccuggg ccgcaggugg cacgcgcugu cgcgagaaga gcaggccaag     4140

uacuaugagc uggcccgcaa ggagaggcag cugcacaugc agcuauaccc aggcugguca     4200

gcgcgggaca acuacgggaa gaagaagagg cggucgaggg aaaagcacca agaauccacc     4260

acaggaggaa aaagaaaugc auucgguacu uacccggaga aggccgcugc cccagccccg     4320

uuccuuccga ugacagugcu cuag                                            4344


<210>  8
<211>  8477
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FUW-tetO-MCS - addgene plasmid 84008

<400>  8
cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc       60

aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt      120

aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta      180

tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg      240

gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga      300

cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt      360

tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg      420

gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc      480

cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg      540

taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat      600

aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc      660

tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc      720

aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt      780

agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa      840

accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga      900

ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga      960

tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg     1020

gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga     1080

gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat     1140

actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa     1200

tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc     1260

tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc     1320

tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat     1380

ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg     1440

tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag     1500

caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt     1560

ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt     1620

tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat     1680

acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca     1740

ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca     1800

cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa     1860

ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat     1920

gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca     1980

taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga     2040

atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg     2100

gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca     2160

ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc     2220

atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta     2280

gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa     2340

aattttcggg tttattacag ggacagcaga gatccagttt ggttatcgag tttaccactc     2400

cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa     2460

aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta     2520

ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat     2580

agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg     2640

agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagctcggt acccgggtcg     2700

aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat     2760

cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag     2820

cctccgcggc cccgaattac aggctagcta tcaggatcct acgaggcgcg ccagatagaa     2880

ttctcttggg cctcaagggc catgagttaa ccagttctag atgtgactca tgtcaattcg     2940

atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta     3000

ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc     3060

atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt     3120

ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg     3180

ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt     3240

tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct     3300

ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt     3360

cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct     3420

acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc     3480

ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct     3540

ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca     3600

atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg     3660

gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc     3720

ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac     3780

aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact     3840

acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac     3900

cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc     3960

ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca     4020

gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct     4080

ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc     4140

ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg     4200

gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt     4260

taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc     4320

tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat     4380

gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg     4440

caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc     4500

tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc     4560

tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt     4620

gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc     4680

ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta     4740

cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc     4800

tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg     4860

ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt     4920

ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat     4980

taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca     5040

gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct     5100

ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc     5160

ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg     5220

gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc     5280

agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt     5340

gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat     5400

cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt     5460

tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg     5520

gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct     5580

gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt     5640

gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga     5700

cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct     5760

gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct     5820

acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg     5880

ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc     5940

caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac     6000

aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc     6060

ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct     6120

gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat     6180

aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc     6240

actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg     6300

cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct     6360

gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt     6420

atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc     6480

caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga     6540

gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata     6600

ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac     6660

cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg     6720

taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc     6780

cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag     6840

acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt     6900

aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt     6960

atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg     7020

atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac     7080

gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca     7140

gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac     7200

ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac     7260

ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt     7320

tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt     7380

accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt     7440

atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc     7500

cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa     7560

tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg     7620

tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt     7680

gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc     7740

agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt     7800

aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg     7860

gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac     7920

tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc     7980

gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt     8040

tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg     8100

aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag     8160

catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa     8220

acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg     8280

agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta     8340

agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt     8400

taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg     8460

cgttttgcgc tgcttcg                                                    8477


