                         SEQUENCE LISTING

<110>  ApiRays Biosciences AB
 
<120>  MODIFIED SHIGELLA APYRASE AND USES THEREOF

<130>  NP0498WO

<150>  SE 2150297-6
<151>  2021-03-16

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  246
<212>  PRT
<213>  Shigella flexneri

<400>  1

Met Lys Thr Lys Asn Phe Leu Leu Phe Cys Ile Ala Thr Asn Met Ile 
1               5                   10                  15      


Phe Ile Pro Ser Ala Asn Ala Leu Lys Ala Glu Gly Phe Leu Thr Gln 
            20                  25                  30          


Gln Thr Ser Pro Asp Ser Leu Ser Ile Leu Pro Pro Pro Pro Ala Glu 
        35                  40                  45              


Asn Ser Val Val Phe Gln Ala Asp Lys Ala His Tyr Glu Phe Gly Arg 
    50                  55                  60                  


Ser Leu Arg Asp Ala Asn Arg Val Arg Leu Ala Ser Glu Asp Ala Tyr 
65                  70                  75                  80  


Tyr Glu Asn Phe Gly Leu Ala Phe Ser Asp Ala Tyr Gly Met Asp Ile 
                85                  90                  95      


Ser Arg Glu Asn Thr Pro Ile Leu Tyr Gln Leu Leu Thr Gln Val Leu 
            100                 105                 110         


Gln Asp Ser His Asp Tyr Ala Val Arg Asn Ala Lys Glu Tyr Tyr Lys 
        115                 120                 125             


Arg Val Arg Pro Phe Val Ile Tyr Lys Asp Ala Thr Cys Thr Pro Asp 
    130                 135                 140                 


Lys Asp Glu Lys Met Ala Ile Thr Gly Ser Tyr Pro Ser Gly His Ala 
145                 150                 155                 160 


Ser Phe Gly Trp Ala Val Ala Leu Ile Leu Ala Glu Ile Asn Pro Gln 
                165                 170                 175     


Arg Lys Ala Glu Ile Leu Arg Arg Gly Tyr Glu Phe Gly Glu Ser Arg 
            180                 185                 190         


Val Ile Cys Gly Ala His Trp Gln Ser Asp Val Glu Ala Gly Arg Leu 
        195                 200                 205             


Met Gly Ala Ser Val Val Ala Val Leu His Asn Thr Pro Glu Phe Thr 
    210                 215                 220                 


Lys Ser Leu Ser Glu Ala Lys Lys Glu Phe Glu Glu Leu Asn Thr Pro 
225                 230                 235                 240 


Thr Asn Glu Leu Thr Pro 
                245     


<210>  2
<211>  738
<212>  DNA
<213>  artificial

<220>
<223>  Mutated variant

<400>  2
atgaaaacca aaaactttct gctgttttgc attgccacca acatgatttt tatcccgagc       60

gcaaatgcac tgaaagcaga aggttttctg acccagcaga ccagtccgga tagcctgagc      120

attctgcctc cgcctccggc agaaaataac gttgttgttc aggcagataa agcccactat      180

gaatttcgtc gtagcgttcg tgatgcacaa cgtgttcgtc tggcaagcgt agatatctat      240

tatgaaaatt ttggcctggc ctttagtacc gcctatggta tggatattac ccgtgaaaat      300

accccgattc tgtatcagct gatgacccag gttctgaacg atagccatga ttatgcacgt      360

cgtaatgcca gagagtacca gaaacgcgtt cgtccgtttg tgatctataa agatgcaacc      420

tgtacacgtg ataaagatga aaaaatggca attctgggta gctatccgag cggtcatgca      480

agcaacggtt gggcagttgg gctgattctg gcagaaattc aaccgcagcg caaagcagaa      540

atttttcgtg ccggttatga attcggtgaa agccgtgtta tttgtggtgc acattatcag      600

agtgatgttg aagcacgccg tctgatgggt gcaagcgctg ttgcagttct gcatcgaaca      660

ccggaattga ccaaaagctc gagcgaagcc aaaaaagaat atgaagaact gaatcaaccg      720

accaatgatc tgacaccg                                                    738


<210>  3
<211>  254
<212>  PRT
<213>  Artificial

<220>
<223>  Mutated variant

<400>  3

Met Lys Thr Lys Asn Phe Leu Leu Phe Cys Ile Ala Thr Asn Met Ile 
1               5                   10                  15      


Phe Ile Pro Ser Ala Asn Ala Leu Lys Ala Glu Gly Phe Leu Thr Gln 
            20                  25                  30          


Gln Thr Ser Pro Asp Ser Leu Ser Ile Leu Pro Pro Pro Pro Ala Glu 
        35                  40                  45              


Asn Asn Val Val Val Gln Ala Asp Lys Ala His Tyr Glu Phe Arg Arg 
    50                  55                  60                  


Ser Val Arg Asp Ala Gln Arg Val Arg Leu Ala Ser Val Asp Ile Tyr 
65                  70                  75                  80  


Tyr Glu Asn Phe Gly Leu Ala Phe Ser Thr Ala Tyr Gly Met Asp Ile 
                85                  90                  95      


Thr Arg Glu Asn Thr Pro Ile Leu Tyr Gln Leu Met Thr Gln Val Leu 
            100                 105                 110         


Asn Asp Ser His Asp Tyr Ala Arg Arg Asn Ala Arg Glu Tyr Gln Lys 
        115                 120                 125             


Arg Val Arg Pro Phe Val Ile Tyr Lys Asp Ala Thr Cys Thr Arg Asp 
    130                 135                 140                 


Lys Asp Glu Lys Met Ala Leu Thr Gly Ser Tyr Pro Ser Gly His Ala 
145                 150                 155                 160 


Ser Asn Gly Trp Ala Val Gly Leu Ile Leu Ala Glu Ile Gln Pro Gln 
                165                 170                 175     


Arg Arg Ala Glu Ile Phe Arg Arg Ala Tyr Glu Phe Gly Glu Ser Arg 
            180                 185                 190         


Val Ile Cys Gly Ala His Tyr Gln Ser Asp Val Glu Ala Arg Arg Leu 
        195                 200                 205             


Met Gly Ala Ser Ala Val Ala Val Leu His Lys Thr Pro Glu Trp Thr 
    210                 215                 220                 


Lys Ser Ser Ser Glu Ala Lys Lys Glu Tyr Glu Glu Leu Asn Gln Pro 
225                 230                 235                 240 


Thr Asn Asp Leu Thr Pro Leu Glu His His His His His His 
                245                 250                 


<210>  4
<211>  765
<212>  DNA
<213>  Shigella flexneri

<400>  4
atgaaaacca aaaactttct gctgttttgc attgccacca acatgatttt tatcccgagc       60

gcaaatgcac tgaaagcaga aggttttctg acccagcaga ccagtccgga tagcctgagc      120

attctgcctc cgcctccggc agaaaatagc gttgtttttc aggcagataa agcccactat      180

gaatttggtc gtagcctgcg tgatgcaaat cgtgttcgtc tggcaagcga agatgcatat      240

tatgaaaatt ttggcctggc ctttagtgat gcctatggta tggatattag ccgtgaaaat      300

accccgattc tgtatcagct gctgacccag gttctgcagg atagccatga ttatgcagtt      360

cgtaatgcca aagagtacta taaacgcgtt cgtccgtttg tgatctataa agatgcaacc      420

tgtacaccgg ataaagatga aaaaatggca attaccggta gctatccgag cggtcatgca      480

agctttggtt gggcagttgc actgattctg gcagaaatta atccgcagcg taaagcagaa      540

attctgcgtc gtggttatga attcggtgaa agccgtgtta tttgtggtgc acattggcag      600

agtgatgttg aagcaggtcg tctgatgggt gcaagcgttg ttgcagttct gcataataca      660

ccggaattta ccaaaagcct gagcgaagcc aaaaaagaat ttgaagaact gaatacgccg      720

accaatgaac tgacaccgct cgagcaccac caccaccacc actga                      765


