                         SEQUENCE LISTING

<110>  Adrenas Therapeutics, Inc.
 
<120>  GENE THERAPIES FOR 21-HYDROXYLASE DEFICIENCY

<130>  ADRE-002/01WO 334912-2014 

<150>  US 63/163,634
<151>  2021-03-19

<160>  59    

<170>  PatentIn version 3.5

<210>  1
<211>  495
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Leu Leu Leu Gly Leu Leu Leu Leu Leu Pro Leu Leu Ala Gly Ala 
1               5                   10                  15      


Arg Leu Leu Trp Asn Trp Trp Lys Leu Arg Ser Leu His Leu Pro Pro 
            20                  25                  30          


Leu Ala Pro Gly Phe Leu His Leu Leu Gln Pro Asp Leu Pro Ile Tyr 
        35                  40                  45              


Leu Leu Gly Leu Thr Gln Lys Phe Gly Pro Ile Tyr Arg Leu His Leu 
    50                  55                  60                  


Gly Leu Gln Asp Val Val Val Leu Asn Ser Lys Arg Thr Ile Glu Glu 
65                  70                  75                  80  


Ala Met Val Lys Lys Trp Ala Asp Phe Ala Gly Arg Pro Glu Pro Leu 
                85                  90                  95      


Thr Tyr Lys Leu Val Ser Arg Asn Tyr Pro Asp Leu Ser Leu Gly Asp 
            100                 105                 110         


Tyr Ser Leu Leu Trp Lys Ala His Lys Lys Leu Thr Arg Ser Ala Leu 
        115                 120                 125             


Leu Leu Gly Ile Arg Asp Ser Met Glu Pro Val Val Glu Gln Leu Thr 
    130                 135                 140                 


Gln Glu Phe Cys Glu Arg Met Arg Ala Gln Pro Gly Thr Pro Val Ala 
145                 150                 155                 160 


Ile Glu Glu Glu Phe Ser Leu Leu Thr Cys Ser Ile Ile Cys Tyr Leu 
                165                 170                 175     


Thr Phe Gly Asp Lys Ile Lys Asp Asp Asn Leu Met Pro Ala Tyr Tyr 
            180                 185                 190         


Lys Cys Ile Gln Glu Val Leu Lys Thr Trp Ser His Trp Ser Ile Gln 
        195                 200                 205             


Ile Val Asp Val Ile Pro Phe Leu Arg Phe Phe Pro Asn Pro Gly Leu 
    210                 215                 220                 


Arg Arg Leu Lys Gln Ala Ile Glu Lys Arg Asp His Ile Val Glu Met 
225                 230                 235                 240 


Gln Leu Arg Gln His Lys Glu Ser Leu Val Ala Gly Gln Trp Arg Asp 
                245                 250                 255     


Met Met Asp Tyr Met Leu Gln Gly Val Ala Gln Pro Ser Met Glu Glu 
            260                 265                 270         


Gly Ser Gly Gln Leu Leu Glu Gly His Val His Met Ala Ala Val Asp 
        275                 280                 285             


Leu Leu Ile Gly Gly Thr Glu Thr Thr Ala Asn Thr Leu Ser Trp Ala 
    290                 295                 300                 


Val Val Phe Leu Leu His His Pro Glu Ile Gln Gln Arg Leu Gln Glu 
305                 310                 315                 320 


Glu Leu Asp His Glu Leu Gly Pro Gly Ala Ser Ser Ser Arg Val Pro 
                325                 330                 335     


Tyr Lys Asp Arg Ala Arg Leu Pro Leu Leu Asn Ala Thr Ile Ala Glu 
            340                 345                 350         


Val Leu Arg Leu Arg Pro Val Val Pro Leu Ala Leu Pro His Arg Thr 
        355                 360                 365             


Thr Arg Pro Ser Ser Ile Ser Gly Tyr Asp Ile Pro Glu Gly Thr Val 
    370                 375                 380                 


Ile Ile Pro Asn Leu Gln Gly Ala His Leu Asp Glu Thr Val Trp Glu 
385                 390                 395                 400 


Arg Pro His Glu Phe Trp Pro Asp Arg Phe Leu Glu Pro Gly Lys Asn 
                405                 410                 415     


Ser Arg Ala Leu Ala Phe Gly Cys Gly Ala Arg Val Cys Leu Gly Glu 
            420                 425                 430         


Pro Leu Ala Arg Leu Glu Leu Phe Val Val Leu Thr Arg Leu Leu Gln 
        435                 440                 445             


Ala Phe Thr Leu Leu Pro Ser Gly Asp Ala Leu Pro Ser Leu Gln Pro 
    450                 455                 460                 


Leu Pro His Cys Ser Val Ile Leu Lys Met Gln Pro Phe Gln Val Arg 
465                 470                 475                 480 


Leu Gln Pro Arg Gly Met Gly Ala His Ser Pro Gly Gln Ser Gln 
                485                 490                 495 


<210>  2
<211>  584
<212>  DNA
<213>  Unknown

<220>
<223>  CAG promoter

<400>  2
gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat       60

tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc      120

aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc      180

caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt      240

acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta      300

ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac      360

ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg      420

ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga      480

gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc      540

ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcg                       584


<210>  3
<211>  399
<212>  DNA
<213>  Unknown

<220>
<223>  PGK promoter

<400>  3
ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc       60

ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg      120

aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg      180

taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc      240

tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc      300

gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt      360

tctcctcttc ctcatctccg ggcctttcga cctgcagcc                             399


<210>  4
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CYP21A2 transgene primer

<400>  4
acagtcatca ttccgaacct cca                                               23


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CYP21A2 transgene primer

<400>  5
aaggccagag ctctggagtt ctt                                               23


<210>  6
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mouse brain-derived neurotrophic factor primer

<400>  6
tgctggatga ggaccagaag gtt                                               23


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mouse brain-derived neurotrophic factor primer

<400>  7
aggaggctcc aaaggcactt ga                                                22


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mc2r primer

<400>  8
tttctcagtc atcttgccga                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mc2r primer

<400>  9
atgctcctct ccttggcttt                                                   20


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Prkar1a primer

<400>  10
gcattccttc gggaatactt t                                                 21


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Prkar1a primer

<400>  11
ccctcgagtc agtacggatg                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Prkar2a primer

<400>  12
gagtgactcg gactcggaag                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Prkar2a primer

<400>  13
cctcctcttc ttcatcaggg                                                   20


<210>  14
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Prkarca primer

<400>  14
aagaagggca gcgagcag                                                     18


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Prkarca primer

<400>  15
attctgagaa ggggtctccc                                                   20


<210>  16
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Prkarcb primer

<400>  16
caagaaaggc agcgaagtg                                                    19


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Prkarcb primer

<400>  17
tcctcaagcc cagcattact                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sf1 primer

<400>  18
gccaggagtt cgtctgtctc                                                   20


<210>  19
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sf1 primer

<400>  19
tttcctgggc gtcctttac                                                    19


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Star primer

<400>  20
gggcatactc aacaaccagg                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Star primer

<400>  21
gaaacacctt gcccacatct                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyp11a1 primer

<400>  22
caggccaaca ttaccgagat                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyp11a1 primer

<400>  23
ccttcaagtt gtgtgccatc                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hsd3b1 primer

<400>  24
gttgtcatcc acactgctgc                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hsd3b1 primer

<400>  25
caggcctcca ataggttctg                                                   20


<210>  26
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyp11b1 primer

<400>  26
atggactttc agtccagtgt gttc                                              24


<210>  27
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyp11b1 primer

<400>  27
gccgctcccc aaaaagaa                                                     18


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyp17a1 primer

<400>  28
gaagtgctcg tgaagaaggg                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyp17a1 primer

<400>  29
ctactatccg caaaggcgac                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyp11b2 primer

<400>  30
gagacgtggt gtgttcttgc                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cyp11b2 primer

<400>  31
tcccttgcta ccatgtccac                                                   20


<210>  32
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CYP21 primer

<400>  32
acagtcatca ttccgaacct cca                                               23


<210>  33
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CYP21 primer

<400>  33
aaggccagag ctctggagtt ctt                                               23


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Renin primer

<400>  34
ctctgggcac tcttgttgct                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Renin primer

<400>  35
agaaggcatt ttcttgagcg                                                   20


<210>  36
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Tbp primer

<400>  36
cccttgtacc cttcaccaat gac                                               23


<210>  37
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Tbp primer

<400>  37
tcacggtaga tacaatattt tgaagctg                                          28


<210>  38
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  38
aaattcgggc ccatctacag g                                                 21


<210>  39
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  39
atggcttcct caatggtcct c                                                 21


<210>  40
<211>  23
<212>  DNA
<213>  Macaca fascicularis

<400>  40
ctgtcatgct gctgctgaga ctt                                               23


<210>  41
<211>  24
<212>  DNA
<213>  Macaca fascicularis

<400>  41
ctttggcata gcattcatga ggat                                              24


<210>  42
<211>  23
<212>  DNA
<213>  Mus musculus

<400>  42
tgctggatga ggaccagaag gtt                                               23


<210>  43
<211>  22
<212>  DNA
<213>  Mus musculus

<400>  43
aggaggctcc aaaggcactt ga                                                22


<210>  44
<211>  7
<212>  RNA
<213>  Unknown

<220>
<223>  Kozak sequence

<400>  44
accaugg                                                                  7


<210>  45
<211>  13
<212>  RNA
<213>  Unknown

<220>
<223>  Kozak sequence

<400>  45
gccgccacca ugg                                                          13


<210>  46
<211>  8
<212>  RNA
<213>  Unknown

<220>
<223>  Kozak sequence

<400>  46
ccaccaug                                                                 8


<210>  47
<211>  9
<212>  RNA
<213>  Unknown

<220>
<223>  Kozak sequence

<400>  47
ccaccaugg                                                                9


<210>  48
<211>  260
<212>  DNA
<213>  Unknown

<220>
<223>  CB6 Promoter

<400>  48
ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat       60

ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggcgcg cgccaggcgg      120

ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca      180

gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa      240

aaagcgaagc gcgcggcggg                                                  260


<210>  49
<211>  282
<212>  DNA
<213>  Unknown

<220>
<223>  CBA Promoter

<400>  49
tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc       60

caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg      120

gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag      180

gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc      240

ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gg                         282


<210>  50
<211>  1488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon Optimized CYP21a2 gene sequence

<400>  50
atgctgctgc tggggctgct gctgctgctg cctctgctgg ctggggctcg actgctgtgg       60

aactggtgga aactgcggtc cctgcacctg ccacctctgg caccaggctt cctgcacctg      120

ctgcagccag acctgcccat ctacctgctg ggcctgaccc agaagtttgg ccctatctat      180

aggctgcacc tgggcctgca ggacgtggtg gtgctgaact ctaagcgcac catcgaggag      240

gccatggtga agaagtgggc agatttcgca ggccggccag agccactgac atacaagctg      300

gtgagcagaa attatcctga cctgtccctg ggcgattact ctctgctgtg gaaggcccac      360

aagaagctga caaggagcgc cctgctgctg ggcatccgcg actccatgga gccagtggtg      420

gagcagctga cccaggagtt ttgcgagagg atgagggcac agcctggaac accagtggcc      480

atcgaggagg agttcagcct gctgacctgc tccatcatct gttatctgac atttggcgat      540

aagatcaagg acgataacct gatgccagcc tactataagt gtatccagga ggtgctgaag      600

acctggagcc actggagcat ccagatcgtg gacgtgatcc ccttcctgag gttctttcct      660

aatccaggcc tgcggagact gaagcaggcc atcgagaaga gggatcacat cgtggagatg      720

cagctgaggc agcacaagga gtccctggtg gcaggacagt ggagggacat gatggattac      780

atgctgcagg gagtggcaca gccatctatg gaggagggaa gcggacagct gctggaggga      840

cacgtgcaca tggcagcagt ggatctgctg atcggaggaa ccgagacaac agccaacaca      900

ctgagctggg ccgtggtgtt tctgctgcac caccctgaga tccagcagcg gctgcaggag      960

gagctggacc acgagctggg acctggagca agctcctcta gagtgccata caaggatcgg     1020

gccagactgc ccctgctgaa tgccaccatc gccgaggtgc tgaggctgcg ccccgtggtg     1080

cctctggccc tgcctcacag gaccacaaga ccaagctcca tctccggcta tgacatccca     1140

gagggcaccg tgatcatccc aaacctgcag ggagcacacc tggacgagac agtgtgggag     1200

cggccacacg agttctggcc cgatagattt ctggagcctg gcaagaacag ccgggccctg     1260

gccttcggct gcggagcccg ggtgtgcctg ggcgagccac tggccaggct ggagctgttc     1320

gtggtgctga cccgcctgct gcaggccttt acactgctgc cctccggcga tgccctgcct     1380

tctctgcagc cactgcctca ctgctccgtg atcctgaaga tgcagccctt tcaggtccgc     1440

ctgcagccaa gggggatggg ggcacatagt ccagggcagt ctcagtaa                  1488


<210>  51
<211>  592
<212>  DNA
<213>  Marmota monax

<400>  51
aatcaacctc tggattacaa aatttgtgaa agattgactg atattcttaa ctatgttgct       60

ccttttacgc tgtgtggata tgctgcttta atgcctctgt atcatgctat tgcttcccgt      120

acggctttcg ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg      180

tggcccgttg tccgtcaacg tggcgtggtg tgctctgtgt ttgctgacgc aacccccact      240

ggctggggca ttgccaccac ctgtcaactc ctttctggga ctttcgcttt ccccctcccg      300

atcgccacgg cagaactcat cgccgcctgc cttgcccgct gctggacagg ggctaggttg      360

ctgggcactg ataattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc      420

gcctgtgttg ccaactggat cctgcgcggg acgtccttct gctacgtccc ttcggctctc      480

aatccagcgg acctcccttc ccgaggcctt ctgccggttc tgcggcctct cccgcgtctt      540

cgctttcggc ctccgacgag tcggatctcc ctttgggccg cctccccgcc tg              592


<210>  52
<211>  3901
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette


<220>
<221>  misc_feature
<222>  (1)..(130)
<223>  5' ITR

<220>
<221>  misc_feature
<222>  (261)..(642)
<223>  CMVIE Enhancer

<220>
<221>  misc_feature
<222>  (648)..(925)
<223>  CBA Promoter

<220>
<221>  misc_feature
<222>  (926)..(1942)
<223>  Intron

<220>
<221>  misc_feature
<222>  (2003)..(3490)
<223>  hCYP21 Opt

<220>
<221>  misc_feature
<222>  (3624)..(3679)
<223>  B-globin PolyA

<220>
<221>  misc_feature
<222>  (3772)..(3901)
<223>  3' ITR

<400>  52
ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt       60

gcccgggcgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg      180

cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa      240

ctccatcact aggggttcct ctagtcgaca ttgattattg actagttatt aatagtaatc      300

aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt      360

aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta      420

tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg      480

gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga      540

cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt      600

tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc      660

cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt      720

tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg      780

cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa      840

tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta      900

taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg      960

ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt     1020

gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct     1080

tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg     1140

ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc     1200

cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag     1260

tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg     1320

gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc     1380

ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc     1440

gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg     1500

gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg     1560

gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct     1620

tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc     1680

gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc     1740

cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct     1800

ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag     1860

ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca     1920

tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca     1980

ttttggcaaa gaattcgcca ccatgctgct gctggggctg ctgctgctgc tgcctctgct     2040

ggctggggct cgactgctgt ggaactggtg gaaactgcgg tccctgcacc tgccacctct     2100

ggcaccaggc ttcctgcacc tgctgcagcc agacctgccc atctacctgc tgggcctgac     2160

ccagaagttt ggccctatct ataggctgca cctgggcctg caggacgtgg tggtgctgaa     2220

ctctaagcgc accatcgagg aggccatggt gaagaagtgg gcagatttcg caggccggcc     2280

agagccactg acatacaagc tggtgagcag aaattatcct gacctgtccc tgggcgatta     2340

ctctctgctg tggaaggccc acaagaagct gacaaggagc gccctgctgc tgggcatccg     2400

cgactccatg gagccagtgg tggagcagct gacccaggag ttttgcgaga ggatgagggc     2460

acagcctgga acaccagtgg ccatcgagga ggagttcagc ctgctgacct gctccatcat     2520

ctgttatctg acatttggcg ataagatcaa ggacgataac ctgatgccag cctactataa     2580

gtgtatccag gaggtgctga agacctggag ccactggagc atccagatcg tggacgtgat     2640

ccccttcctg aggttctttc ctaatccagg cctgcggaga ctgaagcagg ccatcgagaa     2700

gagggatcac atcgtggaga tgcagctgag gcagcacaag gagtccctgg tggcaggaca     2760

gtggagggac atgatggatt acatgctgca gggagtggca cagccatcta tggaggaggg     2820

aagcggacag ctgctggagg gacacgtgca catggcagca gtggatctgc tgatcggagg     2880

aaccgagaca acagccaaca cactgagctg ggccgtggtg tttctgctgc accaccctga     2940

gatccagcag cggctgcagg aggagctgga ccacgagctg ggacctggag caagctcctc     3000

tagagtgcca tacaaggatc gggccagact gcccctgctg aatgccacca tcgccgaggt     3060

gctgaggctg cgccccgtgg tgcctctggc cctgcctcac aggaccacaa gaccaagctc     3120

catctccggc tatgacatcc cagagggcac cgtgatcatc ccaaacctgc agggagcaca     3180

cctggacgag acagtgtggg agcggccaca cgagttctgg cccgatagat ttctggagcc     3240

tggcaagaac agccgggccc tggccttcgg ctgcggagcc cgggtgtgcc tgggcgagcc     3300

actggccagg ctggagctgt tcgtggtgct gacccgcctg ctgcaggcct ttacactgct     3360

gccctccggc gatgccctgc cttctctgca gccactgcct cactgctccg tgatcctgaa     3420

gatgcagccc tttcaggtcc gcctgcagcc aagggggatg ggggcacata gtccagggca     3480

gtctcagtaa acgcgtggta cctctagagt cgacccgggc ggcctcgagg acggggtgaa     3540

ctacgcctga ggatccgatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc     3600

ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg     3660

gaattttttg tgtctctcac tcggaagcaa ttcgttgatc tgaatttcga ccacccataa     3720

tacccattac cctggtagat aagtagcatg gcgggttaat cattaactac aaggaacccc     3780

tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac     3840

caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca     3900

g                                                                     3901


<210>  53
<211>  130
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' ITR

<400>  53
ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt       60

gcccgggcgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct                                                             130


<210>  54
<211>  382
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CMVIE Enhancer

<400>  54
ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc       60

atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac      120

cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa      180

tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag      240

tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc      300

ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct      360

acgtattagt catcgctatt ac                                               382


<210>  55
<211>  278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CBA Promoter

<400>  55
tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa       60

ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg      120

ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg      180

cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc      240

ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcg                              278


<210>  56
<211>  1017
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Intron

<400>  56
ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg       60

ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct      120

ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa      180

agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc      240

gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc      300

tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg      360

ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg      420

tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac      480

ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt      540

ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg      600

gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc      660

ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg      720

cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc      780

ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag      840

ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg      900

cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg      960

accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacag        1017


<210>  57
<211>  1488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hCYP21 Opt

<400>  57
atgctgctgc tggggctgct gctgctgctg cctctgctgg ctggggctcg actgctgtgg       60

aactggtgga aactgcggtc cctgcacctg ccacctctgg caccaggctt cctgcacctg      120

ctgcagccag acctgcccat ctacctgctg ggcctgaccc agaagtttgg ccctatctat      180

aggctgcacc tgggcctgca ggacgtggtg gtgctgaact ctaagcgcac catcgaggag      240

gccatggtga agaagtgggc agatttcgca ggccggccag agccactgac atacaagctg      300

gtgagcagaa attatcctga cctgtccctg ggcgattact ctctgctgtg gaaggcccac      360

aagaagctga caaggagcgc cctgctgctg ggcatccgcg actccatgga gccagtggtg      420

gagcagctga cccaggagtt ttgcgagagg atgagggcac agcctggaac accagtggcc      480

atcgaggagg agttcagcct gctgacctgc tccatcatct gttatctgac atttggcgat      540

aagatcaagg acgataacct gatgccagcc tactataagt gtatccagga ggtgctgaag      600

acctggagcc actggagcat ccagatcgtg gacgtgatcc ccttcctgag gttctttcct      660

aatccaggcc tgcggagact gaagcaggcc atcgagaaga gggatcacat cgtggagatg      720

cagctgaggc agcacaagga gtccctggtg gcaggacagt ggagggacat gatggattac      780

atgctgcagg gagtggcaca gccatctatg gaggagggaa gcggacagct gctggaggga      840

cacgtgcaca tggcagcagt ggatctgctg atcggaggaa ccgagacaac agccaacaca      900

ctgagctggg ccgtggtgtt tctgctgcac caccctgaga tccagcagcg gctgcaggag      960

gagctggacc acgagctggg acctggagca agctcctcta gagtgccata caaggatcgg     1020

gccagactgc ccctgctgaa tgccaccatc gccgaggtgc tgaggctgcg ccccgtggtg     1080

cctctggccc tgcctcacag gaccacaaga ccaagctcca tctccggcta tgacatccca     1140

gagggcaccg tgatcatccc aaacctgcag ggagcacacc tggacgagac agtgtgggag     1200

cggccacacg agttctggcc cgatagattt ctggagcctg gcaagaacag ccgggccctg     1260

gccttcggct gcggagcccg ggtgtgcctg ggcgagccac tggccaggct ggagctgttc     1320

gtggtgctga cccgcctgct gcaggccttt acactgctgc cctccggcga tgccctgcct     1380

tctctgcagc cactgcctca ctgctccgtg atcctgaaga tgcagccctt tcaggtccgc     1440

ctgcagccaa gggggatggg ggcacatagt ccagggcagt ctcagtaa                  1488


<210>  58
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B-globin PolyA

<400>  58
aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctca           56


<210>  59
<211>  130
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' ITR

<400>  59
aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg       60

ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc      120

gagcgcgcag                                                             130


