                         SEQUENCE LISTING

<110>  Brigham Young University
 
<120>  GALECTIN-1 IMMUNOMODULATION AND MYOGENIC IMPROVEMENTS IN MUSCLE 
       DISEASES AND AUTOIMMUNE DISORDERS

<130>  82570-355126

<150>  63/161,027
<151>  2021-03-15

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  408
<212>  PRT
<213>  Homo sapiens

<400>  1

Ala Thr Gly Gly Cys Thr Thr Gly Thr Gly Gly Thr Cys Thr Gly Gly 
1               5                   10                  15      


Thr Cys Gly Cys Cys Ala Gly Cys Ala Ala Cys Cys Thr Gly Ala Ala 
            20                  25                  30          


Thr Cys Thr Cys Ala Ala Ala Cys Cys Thr Gly Gly Ala Gly Ala Gly 
        35                  40                  45              


Thr Gly Cys Cys Thr Thr Cys Gly Ala Gly Thr Gly Cys Gly Ala Gly 
    50                  55                  60                  


Gly Cys Gly Ala Gly Gly Thr Gly Gly Cys Thr Cys Cys Thr Gly Ala 
65                  70                  75                  80  


Cys Gly Cys Thr Ala Ala Gly Ala Gly Cys Thr Thr Cys Gly Thr Gly 
                85                  90                  95      


Cys Thr Gly Ala Ala Cys Cys Thr Gly Gly Gly Cys Ala Ala Ala Gly 
            100                 105                 110         


Ala Cys Ala Gly Cys Ala Ala Cys Ala Ala Cys Cys Thr Gly Thr Gly 
        115                 120                 125             


Cys Cys Thr Gly Cys Ala Cys Thr Thr Cys Ala Ala Cys Cys Cys Thr 
    130                 135                 140                 


Cys Gly Cys Thr Thr Cys Ala Ala Cys Gly Cys Cys Cys Ala Cys Gly 
145                 150                 155                 160 


Gly Cys Gly Ala Cys Gly Cys Cys Ala Ala Cys Ala Cys Cys Ala Thr 
                165                 170                 175     


Cys Gly Thr Gly Thr Gly Cys Ala Ala Cys Ala Gly Cys Ala Ala Gly 
            180                 185                 190         


Gly Ala Cys Gly Gly Cys Gly Gly Gly Gly Cys Cys Thr Gly Gly Gly 
        195                 200                 205             


Gly Gly Ala Cys Cys Gly Ala Gly Cys Ala Gly Cys Gly Gly Gly Ala 
    210                 215                 220                 


Gly Gly Cys Thr Gly Thr Cys Thr Thr Thr Cys Cys Cys Thr Thr Cys 
225                 230                 235                 240 


Cys Ala Gly Cys Cys Thr Gly Gly Ala Ala Gly Thr Gly Thr Thr Gly 
                245                 250                 255     


Cys Ala Gly Ala Gly Gly Thr Gly Thr Gly Cys Ala Thr Cys Ala Cys 
            260                 265                 270         


Cys Thr Thr Cys Gly Ala Cys Cys Ala Gly Gly Cys Cys Ala Ala Cys 
        275                 280                 285             


Cys Thr Gly Ala Cys Cys Gly Thr Cys Ala Ala Gly Cys Thr Gly Cys 
    290                 295                 300                 


Cys Ala Gly Ala Thr Gly Gly Ala Thr Ala Cys Gly Ala Ala Thr Thr 
305                 310                 315                 320 


Cys Ala Ala Gly Thr Thr Cys Cys Cys Cys Ala Ala Cys Cys Gly Cys 
                325                 330                 335     


Cys Thr Cys Ala Ala Cys Cys Thr Gly Gly Ala Gly Gly Cys Cys Ala 
            340                 345                 350         


Thr Cys Ala Ala Cys Thr Ala Cys Ala Thr Gly Gly Cys Ala Gly Cys 
        355                 360                 365             


Thr Gly Ala Cys Gly Gly Thr Gly Ala Cys Thr Thr Cys Ala Ala Gly 
    370                 375                 380                 


Ala Thr Cys Ala Ala Ala Thr Gly Thr Gly Thr Gly Gly Cys Cys Thr 
385                 390                 395                 400 


Thr Thr Gly Ala Cys Thr Gly Ala 
                405             


<210>  2
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinany galectin-1: rHsGal-1

<400>  2

Gly Thr Thr Thr Ala Ala Cys Thr Thr Thr Ala Ala Gly Ala Ala Gly 
1               5                   10                  15      


Gly Ala Gly Ala Thr Ala Thr Ala Cys Ala Cys Ala Thr Gly Gly Cys 
            20                  25                  30          


Thr Thr Gly Thr Gly Gly Thr Cys Thr Gly Gly Thr Cys Gly Cys Cys 
        35                  40                  45              


Ala Gly Cys Ala Ala Cys Cys Thr Gly Ala Ala Thr Cys Thr Cys Ala 
    50                  55                  60                  


Ala Ala Cys Cys Thr Gly Gly Ala Gly Ala Gly Thr Gly Cys Cys Thr 
65                  70                  75                  80  


Thr Cys Gly Ala Gly Thr Gly Cys Gly Ala Gly Gly Cys Gly Ala Gly 
                85                  90                  95      


Gly Thr Gly Gly Cys Thr Cys Cys Thr Gly Ala Cys Gly Cys Thr Ala 
            100                 105                 110         


Ala Gly Ala Gly Cys Thr Thr Cys Gly Thr Gly Cys Thr Gly Ala Ala 
        115                 120                 125             


Cys Cys Thr Gly Gly Gly Cys Ala Ala Ala Gly Ala Cys Ala Gly Cys 
    130                 135                 140                 


Ala Ala Cys Ala Ala Cys Cys Thr Gly Thr Gly Cys Cys Thr Gly Cys 
145                 150                 155                 160 


Ala Cys Thr Thr Cys Ala Ala Cys Cys Cys Thr Cys Gly Cys Thr Thr 
                165                 170                 175     


Cys Ala Ala Cys Gly Cys Cys Cys Ala Cys Gly Gly Cys Gly Ala Cys 
            180                 185                 190         


Gly Cys Cys Ala Ala Cys Ala Cys Cys Ala Thr Cys Gly Thr Gly Thr 
        195                 200                 205             


Gly Cys Ala Ala Cys Ala Gly Cys Ala Ala Gly Gly Ala Cys Gly Gly 
    210                 215                 220                 


Cys Gly Gly Gly Gly Cys Cys Thr Gly Gly Gly Gly Gly Ala Cys Cys 
225                 230                 235                 240 


Gly Ala Gly Cys Ala Gly Cys Gly Gly Gly Ala Gly Gly Cys Thr Gly 
                245                 250                 255     


Thr Cys Thr Thr Thr Cys Cys Cys Thr Thr Cys Cys Ala Gly Cys Cys 
            260                 265                 270         


Thr Gly Gly Ala Ala Gly Thr Gly Thr Thr Gly Cys Ala Gly Ala Gly 
        275                 280                 285             


Gly Thr Gly Thr Gly Cys Ala Thr Cys Ala Cys Cys Thr Thr Cys Gly 
    290                 295                 300                 


Ala Cys Cys Ala Gly Gly Cys Cys Ala Ala Cys Cys Thr Gly Ala Cys 
305                 310                 315                 320 


Cys Gly Thr Cys Ala Ala Gly Cys Thr Gly Cys Cys Ala Gly Ala Thr 
                325                 330                 335     


Gly Gly Ala Thr Ala Cys Gly Ala Ala Thr Thr Cys Ala Ala Gly Thr 
            340                 345                 350         


Thr Cys Cys Cys Cys Ala Ala Cys Cys Gly Cys Cys Thr Cys Ala Ala 
        355                 360                 365             


Cys Cys Thr Gly Gly Ala Gly Gly Cys Cys Ala Thr Cys Ala Ala Cys 
    370                 375                 380                 


Thr Ala Cys Ala Thr Gly Gly Cys Ala Gly Cys Thr Gly Ala Cys Gly 
385                 390                 395                 400 


Gly Thr Gly Ala Cys Thr Thr Cys Ala Ala Gly Ala Thr Cys Ala Ala 
                405                 410                 415     


Ala Thr Gly Thr Gly Thr Gly Gly Cys Cys Thr Thr Thr Gly Ala Cys 
            420                 425                 430         


Gly Gly Cys Thr Cys Cys Cys Ala Thr Cys Ala Cys Cys Ala Thr Cys 
        435                 440                 445             


Ala Cys Cys Ala Thr Cys Ala Cys Thr Gly Ala Cys Ala Thr Ala Thr 
    450                 455                 460                 


Gly Ala Ala Ala Gly Ala Ala Ala Cys Cys Gly Cys Thr Gly Cys Thr 
465                 470                 475                 480 


Gly Cys 
        


<210>  3
<211>  831
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Galectin-1 fixed dimer

<400>  3

Cys Thr Cys Gly Ala Gly Ala Thr Gly Gly Cys Thr Thr Gly Cys Gly 
1               5                   10                  15      


Gly Cys Thr Thr Gly Gly Thr Ala Gly Cys Thr Ala Gly Thr Ala Ala 
            20                  25                  30          


Thr Cys Thr Thr Ala Ala Cys Thr Thr Gly Ala Ala Ala Cys Cys Cys 
        35                  40                  45              


Gly Gly Ala Gly Ala Gly Thr Gly Thr Cys Thr Gly Cys Gly Ala Gly 
    50                  55                  60                  


Thr Gly Ala Gly Ala Gly Gly Ala Gly Ala Gly Gly Thr Gly Gly Cys 
65                  70                  75                  80  


Thr Cys Cys Cys Gly Ala Cys Gly Cys Cys Ala Ala Gly Ala Gly Thr 
                85                  90                  95      


Thr Thr Cys Gly Thr Gly Cys Thr Cys Ala Ala Cys Cys Thr Gly Gly 
            100                 105                 110         


Gly Ala Ala Ala Ala Gly Ala Cys Thr Cys Cys Ala Ala Thr Ala Ala 
        115                 120                 125             


Cys Thr Thr Gly Thr Gly Thr Cys Thr Cys Cys Ala Cys Thr Thr Cys 
    130                 135                 140                 


Ala Ala Thr Cys Cys Thr Ala Gly Gly Thr Thr Thr Ala Ala Thr Gly 
145                 150                 155                 160 


Cys Cys Cys Ala Thr Gly Gly Gly Gly Ala Cys Gly Cys Thr Ala Ala 
                165                 170                 175     


Cys Ala Cys Ala Ala Thr Cys Gly Thr Gly Thr Gly Cys Ala Ala Cys 
            180                 185                 190         


Ala Gly Cys Ala Ala Gly Gly Ala Thr Gly Gly Gly Gly Gly Cys Gly 
        195                 200                 205             


Cys Cys Thr Gly Gly Gly Gly Cys Ala Cys Cys Gly Ala Gly Cys Ala 
    210                 215                 220                 


Gly Cys Gly Ala Gly Ala Gly Gly Cys Cys Gly Thr Thr Thr Thr Cys 
225                 230                 235                 240 


Cys Cys Gly Thr Thr Cys Cys Ala Ala Cys Cys Cys Gly Gly Gly Thr 
                245                 250                 255     


Cys Cys Gly Thr Ala Gly Cys Thr Gly Ala Gly Gly Thr Thr Thr Gly 
            260                 265                 270         


Cys Ala Thr Cys Ala Cys Cys Thr Thr Cys Gly Ala Cys Cys Ala Gly 
        275                 280                 285             


Gly Cys Cys Ala Ala Cys Cys Thr Gly Ala Cys Ala Gly Thr Gly Ala 
    290                 295                 300                 


Ala Gly Cys Thr Cys Cys Cys Cys Gly Ala Thr Gly Gly Ala Thr Ala 
305                 310                 315                 320 


Thr Gly Ala Gly Thr Thr Thr Ala Ala Ala Thr Thr Cys Cys Cys Thr 
                325                 330                 335     


Ala Ala Thr Cys Gly Thr Cys Thr Gly Ala Ala Thr Cys Thr Gly Gly 
            340                 345                 350         


Ala Ala Gly Cys Cys Ala Thr Thr Ala Ala Cys Thr Ala Cys Ala Thr 
        355                 360                 365             


Gly Gly Cys Cys Gly Cys Cys Gly Ala Cys Gly Gly Thr Gly Ala Cys 
    370                 375                 380                 


Thr Thr Thr Ala Ala Ala Ala Thr Ala Ala Ala Gly Thr Gly Cys Gly 
385                 390                 395                 400 


Thr Gly Gly Cys Ala Thr Thr Cys Gly Ala Cys Gly Gly Ala Gly Gly 
                405                 410                 415     


Cys Ala Thr Gly Gly Cys Thr Thr Gly Cys Gly Gly Cys Cys Thr Cys 
            420                 425                 430         


Gly Thr Thr Gly Cys Cys Thr Cys Cys Ala Ala Cys Cys Thr Cys Ala 
        435                 440                 445             


Ala Thr Cys Thr Thr Ala Ala Gly Cys Cys Ala Gly Gly Thr Gly Ala 
    450                 455                 460                 


Gly Thr Gly Cys Cys Thr Gly Cys Gly Cys Gly Thr Ala Ala Gly Ala 
465                 470                 475                 480 


Gly Gly Cys Gly Ala Gly Gly Thr Cys Gly Cys Cys Cys Cys Cys Gly 
                485                 490                 495     


Ala Cys Gly Cys Thr Ala Ala Gly Thr Cys Ala Thr Thr Cys Gly Thr 
            500                 505                 510         


Gly Cys Thr Cys Ala Ala Thr Thr Thr Gly Gly Gly Cys Ala Ala Ala 
        515                 520                 525             


Gly Ala Thr Thr Cys Ala Ala Ala Thr Ala Ala Thr Cys Thr Gly Thr 
    530                 535                 540                 


Gly Cys Cys Thr Thr Cys Ala Cys Thr Thr Cys Ala Ala Cys Cys Cys 
545                 550                 555                 560 


Cys Cys Gly Gly Thr Thr Thr Ala Ala Cys Gly Cys Thr Cys Ala Cys 
                565                 570                 575     


Gly Gly Cys Gly Ala Cys Gly Cys Thr Ala Ala Thr Ala Cys Thr Ala 
            580                 585                 590         


Thr Ala Gly Thr Gly Thr Gly Thr Ala Ala Cys Thr Cys Thr Ala Ala 
        595                 600                 605             


Ala Gly Ala Cys Gly Gly Ala Gly Gly Ala Gly Cys Cys Thr Gly Gly 
    610                 615                 620                 


Gly Gly Cys Ala Cys Thr Gly Ala Ala Cys Ala Gly Ala Gly Ala Gly 
625                 630                 635                 640 


Ala Gly Gly Cys Gly Gly Thr Cys Thr Thr Thr Cys Cys Thr Thr Thr 
                645                 650                 655     


Thr Cys Ala Gly Cys Cys Thr Gly Gly Cys Thr Cys Ala Gly Thr Thr 
            660                 665                 670         


Gly Cys Cys Gly Ala Gly Gly Thr Gly Thr Gly Thr Ala Thr Cys Ala 
        675                 680                 685             


Cys Thr Thr Thr Thr Gly Ala Thr Cys Ala Gly Gly Cys Ala Ala Ala 
    690                 695                 700                 


Cys Cys Thr Cys Ala Cys Cys Gly Thr Gly Ala Ala Ala Cys Thr Cys 
705                 710                 715                 720 


Cys Cys Cys Gly Ala Thr Gly Gly Ala Thr Ala Cys Gly Ala Gly Thr 
                725                 730                 735     


Thr Thr Ala Ala Ala Thr Thr Cys Cys Cys Gly Ala Ala Cys Cys Gly 
            740                 745                 750         


Cys Cys Thr Gly Ala Ala Cys Cys Thr Gly Gly Ala Ala Gly Cys Thr 
        755                 760                 765             


Ala Thr Thr Ala Ala Cys Thr Ala Thr Ala Thr Gly Gly Cys Gly Gly 
    770                 775                 780                 


Cys Ala Gly Ala Cys Gly Gly Ala Gly Ala Thr Thr Thr Thr Ala Ala 
785                 790                 795                 800 


Ala Ala Thr Cys Ala Ala Ala Thr Gly Thr Gly Thr Thr Gly Cys Thr 
                805                 810                 815     


Thr Thr Cys Gly Ala Cys Thr Ala Gly Cys Ala Thr Ala Thr Gly 
            820                 825                 830     


<210>  4
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rHsGal-1 alkylated

<400>  4

Gly Thr Thr Thr Ala Ala Cys Thr Thr Thr Ala Ala Gly Ala Ala Gly 
1               5                   10                  15      


Gly Ala Gly Ala Thr Ala Thr Ala Cys Ala Cys Ala Thr Gly Gly Cys 
            20                  25                  30          


Thr Thr Gly Thr Gly Gly Thr Cys Thr Gly Gly Thr Cys Gly Cys Cys 
        35                  40                  45              


Ala Gly Cys Ala Ala Cys Cys Thr Gly Ala Ala Thr Cys Thr Cys Ala 
    50                  55                  60                  


Ala Ala Cys Cys Thr Gly Gly Ala Gly Ala Gly Thr Gly Cys Cys Thr 
65                  70                  75                  80  


Thr Cys Gly Ala Gly Thr Gly Cys Gly Ala Gly Gly Cys Gly Ala Gly 
                85                  90                  95      


Gly Thr Gly Gly Cys Thr Cys Cys Thr Gly Ala Cys Gly Cys Thr Ala 
            100                 105                 110         


Ala Gly Ala Gly Cys Thr Thr Cys Gly Thr Gly Cys Thr Gly Ala Ala 
        115                 120                 125             


Cys Cys Thr Gly Gly Gly Cys Ala Ala Ala Gly Ala Cys Ala Gly Cys 
    130                 135                 140                 


Ala Ala Cys Ala Ala Cys Cys Thr Gly Thr Gly Cys Cys Thr Gly Cys 
145                 150                 155                 160 


Ala Cys Thr Thr Cys Ala Ala Cys Cys Cys Thr Cys Gly Cys Thr Thr 
                165                 170                 175     


Cys Ala Ala Cys Gly Cys Cys Cys Ala Cys Gly Gly Cys Gly Ala Cys 
            180                 185                 190         


Gly Cys Cys Ala Ala Cys Ala Cys Cys Ala Thr Cys Gly Thr Gly Thr 
        195                 200                 205             


Gly Cys Ala Ala Cys Ala Gly Cys Ala Ala Gly Gly Ala Cys Gly Gly 
    210                 215                 220                 


Cys Gly Gly Gly Gly Cys Cys Thr Gly Gly Gly Gly Gly Ala Cys Cys 
225                 230                 235                 240 


Gly Ala Gly Cys Ala Gly Cys Gly Gly Gly Ala Gly Gly Cys Thr Gly 
                245                 250                 255     


Thr Cys Thr Thr Thr Cys Cys Cys Thr Thr Cys Cys Ala Gly Cys Cys 
            260                 265                 270         


Thr Gly Gly Ala Ala Gly Thr Gly Thr Thr Gly Cys Ala Gly Ala Gly 
        275                 280                 285             


Gly Thr Gly Thr Gly Cys Ala Thr Cys Ala Cys Cys Thr Thr Cys Gly 
    290                 295                 300                 


Ala Cys Cys Ala Gly Gly Cys Cys Ala Ala Cys Cys Thr Gly Ala Cys 
305                 310                 315                 320 


Cys Gly Thr Cys Ala Ala Gly Cys Thr Gly Cys Cys Ala Gly Ala Thr 
                325                 330                 335     


Gly Gly Ala Thr Ala Cys Gly Ala Ala Thr Thr Cys Ala Ala Gly Thr 
            340                 345                 350         


Thr Cys Cys Cys Cys Ala Ala Cys Cys Gly Cys Cys Thr Cys Ala Ala 
        355                 360                 365             


Cys Cys Thr Gly Gly Ala Gly Gly Cys Cys Ala Thr Cys Ala Ala Cys 
    370                 375                 380                 


Thr Ala Cys Ala Thr Gly Gly Cys Ala Gly Cys Thr Gly Ala Cys Gly 
385                 390                 395                 400 


Gly Thr Gly Ala Cys Thr Thr Cys Ala Ala Gly Ala Thr Cys Ala Ala 
                405                 410                 415     


Ala Thr Gly Thr Gly Thr Gly Gly Cys Cys Thr Thr Thr Gly Ala Cys 
            420                 425                 430         


Gly Gly Cys Thr Cys Cys Cys Ala Thr Cys Ala Cys Cys Ala Thr Cys 
        435                 440                 445             


Ala Cys Cys Ala Thr Cys Ala Cys Thr Gly Ala Cys Ala Thr Ala Thr 
    450                 455                 460                 


Gly Ala Ala Ala Gly Ala Ala Ala Cys Cys Gly Cys Thr Gly Cys Thr 
465                 470                 475                 480 


Gly Cys 
        


<210>  5
<211>  135
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mGal-1

<400>  5

Met Ala Cys Gly Leu Asp Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Arg Val Arg Gly Glu Val Ala Pro Asp Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Ser Asn Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Ala Asn Thr Ile Val Cys Asn Ser Lys 
    50                  55                  60                  


Asp Gly Gly Ala Trp Gly Thr Glu Gln Arg Glu Ala Val Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Val Ala Glu Val Cys Ile Thr Phe Asp Gln Ala Asn 
                85                  90                  95      


Leu Thr Val Lys Leu Pro Asp Gly Tyr Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Leu Glu Ala Ile Asn Tyr Met Ala Ala Asp Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Asp 
    130                 135 


