                         SEQUENCE LISTING

<110>  The University of Hong Kong
 
<120>  HEPATITIS E VIRUS-LIKE PARTICLES AND USES THEREOF

<130>  F22W1515

<150>  US 63/166,698
<151>  2021-03-26

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence for HEV-C1 p241

<400>  1

Ile Val Gln Val Leu Phe Asn Ile Ala Asp Thr Leu Leu Gly Gly Leu 
1               5                   10                  15      


Pro Thr Asp Leu Val Ser Asn Ala Gly Gly Gln Leu Phe Tyr Gly Arg 
            20                  25                  30          


Pro Gln Val Ser Glu Asn Gly Glu Pro Ser Val Lys Leu Tyr Thr Ser 
        35                  40                  45              


Val Glu Ala Ala Gln Leu Asp His Gly Val Thr Ile Pro His Asp Ile 
    50                  55                  60                  


Asp Leu Gly Val Ser Ala Ile Thr Leu Gln Asp Phe Asp Asn Gln His 
65                  70                  75                  80  


Leu Gln Asp Arg Pro Thr Pro Ser Pro Ala Pro Ala Arg Pro Ile Thr 
                85                  90                  95      


Asn Trp Arg Ser Gly Asp Val Val Trp Val Thr Leu Pro Ser Ala Glu 
            100                 105                 110         


Tyr Ala Gln Ser Gln Ser Ala Met Gly Ser His Pro Ala Tyr Trp Ser 
        115                 120                 125             


Glu Glu Ala Thr Ile Ile Asn Val Ala Thr Gly Gln Arg Ala Ala Val 
    130                 135                 140                 


Ser Ser Ile Lys Trp Asp Gln Val Thr Leu Asn Gly Lys Ala Leu His 
145                 150                 155                 160 


Lys Glu Thr His Ser Gly Leu Val Tyr Tyr Gln Leu Pro Leu Met Gly 
                165                 170                 175     


Lys Ile Asn Phe Trp Gln Gln Gly Thr Thr Lys Ala Gly Tyr Thr Tyr 
            180                 185                 190         


Asn Tyr Asn Thr Thr Asp Ser Asp Ser Leu Trp Val Trp Trp Asp Gly 
        195                 200                 205             


Gly Ser Lys Ala Tyr Leu Tyr Ile Ser Thr Tyr Thr Thr Met Leu Gly 
    210                 215                 220                 


Ala Gly Pro Val Asn Ile Thr Gly Leu Gly Ala Val Gly Pro Asn Pro 
225                 230                 235                 240 


Val 
    


<210>  2
<211>  724
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence for HEV-C1 p241

<400>  2
attgttcagg ttttgttcaa tattgcggat accctgttag gcggcctgcc aacggacctt       60

gtttctaacg ctgggggcca actgttctat ggccgaccgc aggtgtctga gaatggtgaa      120

ccatcggtta agctttatac atctgtggaa gccgcccagc tggaccatgg ggttaccatt      180

ccccatgata ttgacttggg tgtgtccgcc atcacactac aggactttga taaccagcat      240

ttgcaggacc gccctacgcc ctcaccagcg ccagctcgcc cgatcacgaa ctggcgctct      300

ggtgatgtgg tgtgggtcac attaccatcg gccgaatatg cgcagtctca gagcgcaatg      360

ggttcccacc cggcctactg gtccgaggag gcgactataa tcaacgtcgc tacgggccaa      420

cgggccgccg tgtctagcat aaagtgggat caggtcactc ttaacggcaa ggccctgcat      480

aaggagactc attcaggctt ggtttattac cagctgccat tgatggggaa gatcaatttt      540

tggcagcagg gtaccaccaa agccggttat acttataatt acaacactac tgattcagac      600

agtttgtggg tgtggtggga tgggggctct aaggcttatc tctatatatc tacttatact      660

actatgctag gtgctggacc tgttaacatc acggggctgg gtgctgtcgg ccccaacccc      720

gttg                                                                   724


<210>  3
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of HEV-A4 p239

<400>  3

Ile Ala Leu Thr Leu Phe Asn Leu Ala Asp Thr Leu Leu Gly Gly Leu 
1               5                   10                  15      


Pro Thr Glu Leu Ile Ser Ser Ala Gly Gly Gln Leu Phe Tyr Ser Arg 
            20                  25                  30          


Pro Val Val Ser Ala Asn Gly Glu Pro Thr Val Lys Leu Tyr Thr Ser 
        35                  40                  45              


Val Glu Asn Ala Gln Gln Asp Lys Gly Ile Ala Ile Pro His Asp Ile 
    50                  55                  60                  


Asp Leu Gly Glu Ser Arg Val Val Ile Gln Asp Tyr Asp Asn Gln His 
65                  70                  75                  80  


Glu Gln Asp Arg Pro Thr Pro Ser Pro Ala Pro Ser Arg Pro Phe Ser 
                85                  90                  95      


Val Leu Arg Ala Asn Asp Val Leu Trp Leu Ser Leu Thr Ala Ala Glu 
            100                 105                 110         


Tyr Asp Gln Thr Thr Tyr Gly Ser Ser Thr Asn Pro Met Tyr Val Ser 
        115                 120                 125             


Asp Thr Val Thr Phe Val Asn Val Ala Thr Gly Ala Gln Gly Val Ala 
    130                 135                 140                 


Arg Ser Leu Asp Trp Ser Lys Val Thr Leu Asp Gly Arg Pro Leu Thr 
145                 150                 155                 160 


Thr Ile Gln Gln Tyr Ser Lys Thr Phe Phe Val Leu Pro Leu Arg Gly 
                165                 170                 175     


Lys Leu Ser Phe Trp Glu Ala Gly Thr Thr Lys Ala Gly Tyr Pro Tyr 
            180                 185                 190         


Asn Tyr Asp Thr Thr Ala Ser Asp Gln Ile Leu Ile Glu Asn Ala Ala 
        195                 200                 205             


Gly His Arg Val Cys Ile Ser Thr Tyr Thr Thr Asn Leu Gly Ser Gly 
    210                 215                 220                 


Pro Val Ser Ile Ser Ala Val Gly Val Leu Ala Pro His Ser Ala 
225                 230                 235                 


<210>  4
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence for HEV-A4 p239

<400>  4
atagcattga ccctgtttaa tcttgctgat acgcttctcg gcgggctccc gacagaatta       60

atttcgtcgg ctggtggcca gctgttttac tctcgccccg tcgtctcagc caatggcgag      120

ccgactgtga aactctacac ttcagtcgag aatgcccagc aggataaggg tatagctatc      180

ccacatgata ttgatcttgg tgagtcccga gtagttattc aggattatga caaccagcat      240

gagcaagatc gccctactcc atctcctgct ccctctcgcc ctttttctgt tcttcgtgct      300

aatgatgtgc tttggctttc acttacagct gctgaatacg atcagactac ctatggctct      360

tctactaatc ctatgtatgt ctctgacacc gtaacatttg ttaatgtggc cactggcgcc      420

cagggggtgg cacgctctct ggactggtcc aaggtcaccc ttgatgggcg cccacttacc      480

actattcagc agtactctaa gactttcttt gtcctacccc tccgtggtaa actttctttt      540

tgggaggctg gtacaactaa agctggctac ccatataatt atgatactac tgccagtgac      600

cagattttga ttgagaatgc ggcaggtcat cgtgtctgta tttctactta cactactaac      660

ttaggttctg ggcctgtctc tatttctgct gttggtgttt tagcacccca ctctgct         717


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  5
cgccctgcct cagtacccga t                                                 21


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  6
acgtagactc ctcttgtggc                                                   20


<210>  7
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  7
cgatgtctag caagcccaa                                                    19


<210>  8
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  n is a, c, g, or t

<400>  8
cgactgaggc ytcnaattat gc                                                22


<210>  9
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  9
tgcacrtcct gcatraacc                                                    19


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  10
attcagtgcc acaggaggag t                                                 21


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  11
tggagtatag gaacctgaca c                                                 21


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  12
agagcgccag ttcgtgatcg g                                                 21


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  13
tgtcaggttc atgcaggacg t                                                 21


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  14
gataagcctt agagccccca t                                                 21


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  15
gaagatagcc cggccaaagc a                                                 21


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  16
tggggaagat caatttttgg c                                                 21


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  17
tgggggctct aaggcttatc t                                                 21


<210>  18
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  18
gaccacgcgt atcgatgtcg actttttttt ttttttttv                              39


<210>  19
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  19
catatgatag cattgaccct gtttaatct                                         29


<210>  20
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  20
ctcgagagca gagtggggtg ctaaaacac                                         29


<210>  21
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  21
catatgattg ttcaggtttt gttcaatat                                         29


<210>  22
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  22
ctcgagaacg gggttggggc cgacagcac                                         29


<210>  23
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  23
cttgttgagc tyttctcccc t                                                 21


<210>  24
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  24
ctgtaccgga tgcgaccaa                                                    19


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe sequence

<400>  25
tgcagcttgt ctttgarccc                                                   20


<210>  26
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  26
cagcggctac cgcctttgct aatgctcagg t                                      31


<210>  27
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  27
gcggcggacg tacgcctcca gaaaatyatg aata                                   34


<210>  28
<211>  149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acids sequence of HEV-A genotype 3

<400>  28

Ser Pro Ala Pro Ser Arg Pro Phe Ser Val Leu Arg Ala Asn Asp Val 
1               5                   10                  15      


Leu Trp Leu Ser Leu Thr Ala Ala Glu Tyr Asp Gln Thr Thr Tyr Gly 
            20                  25                  30          


Ser Ser Thr Asn Pro Met Tyr Val Ser Asp Thr Val Thr Phe Val Asn 
        35                  40                  45              


Val Ala Thr Gly Ala Gln Ala Val Ala Arg Ser Leu Asp Trp Ser Lys 
    50                  55                  60                  


Val Thr Leu Asp Gly Arg Pro Leu Thr Thr Ile Gln Gln Tyr Ser Lys 
65                  70                  75                  80  


Thr Phe Tyr Val Leu Pro Leu Arg Gly Lys Leu Ser Phe Trp Glu Ala 
                85                  90                  95      


Gly Thr Thr Lys Ala Gly Tyr Pro Tyr Asn Tyr Asn Thr Thr Ala Ser 
            100                 105                 110         


Asp Gln Ile Leu Ile Glu Asn Ala Ala Gly His Arg Val Ala Ile Ser 
        115                 120                 125             


Thr Tyr Thr Thr Ser Leu Gly Ala Gly Pro Val Ser Val Ser Ala Val 
    130                 135                 140                 


Gly Val Leu Ala Pro 
145                 


<210>  29
<211>  149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acids sequence of HEV-A genotype 4

<400>  29

Ser Pro Ala Pro Ser Arg Pro Phe Ser Val Leu Arg Ala Asn Asp Val 
1               5                   10                  15      


Leu Trp Leu Ser Leu Thr Ala Ala Glu Tyr Asp Gln Thr Thr Tyr Gly 
            20                  25                  30          


Ser Ser Thr Asn Pro Met Tyr Val Ser Asp Thr Val Thr Phe Val Asn 
        35                  40                  45              


Val Ala Thr Gly Ala Gln Gly Val Ser Arg Ser Leu Asp Trp Ser Lys 
    50                  55                  60                  


Val Thr Leu Asp Gly Arg Pro Leu Thr Thr Ile Gln Gln Tyr Ser Lys 
65                  70                  75                  80  


Thr Phe Tyr Val Leu Pro Leu Arg Gly Lys Leu Ser Phe Trp Glu Ala 
                85                  90                  95      


Gly Thr Thr Lys Ala Gly Tyr Pro Tyr Asn Tyr Asn Thr Thr Ala Ser 
            100                 105                 110         


Asp Gln Ile Leu Ile Glu Asn Ala Ala Gly His Arg Val Cys Ile Ser 
        115                 120                 125             


Thr Tyr Thr Thr Asn Leu Gly Ser Gly Pro Val Ser Val Ser Ala Val 
    130                 135                 140                 


Gly Val Leu Ala Pro 
145                 


<210>  30
<211>  149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acids sequence of HEV-A genotype 1

<400>  30

Ser Pro Ala Pro Ser Arg Pro Phe Ser Val Leu Arg Ala Asn Asp Val 
1               5                   10                  15      


Leu Trp Leu Ser Leu Thr Ala Ala Glu Tyr Asp Gln Ser Thr Tyr Gly 
            20                  25                  30          


Ser Ser Thr Gly Pro Val Tyr Val Ser Asp Ser Val Thr Leu Val Asn 
        35                  40                  45              


Val Ala Thr Gly Ala Gln Ala Val Ala Arg Ser Leu Asp Trp Thr Lys 
    50                  55                  60                  


Val Thr Leu Asp Gly Arg Pro Leu Ser Thr Thr Gln Gln Tyr Ser Lys 
65                  70                  75                  80  


Thr Phe Phe Val Leu Pro Leu Arg Gly Lys Leu Ser Phe Trp Glu Ala 
                85                  90                  95      


Gly Thr Thr Lys Ala Gly Tyr Pro Tyr Asn Tyr Asn Thr Thr Ala Ser 
            100                 105                 110         


Asp Gln Leu Leu Val Glu Asn Ala Ala Gly His Arg Val Ala Ile Ser 
        115                 120                 125             


Thr Tyr Thr Thr Ser Leu Gly Ala Gly Pro Val Ser Ile Ser Ala Val 
    130                 135                 140                 


Ala Val Leu Ala Pro 
145                 


<210>  31
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acids sequence of HEV-C LCK-3110

<400>  31

Ser Pro Ala Pro Ala Arg Pro Ile Thr Asn Trp Arg Ser Gly Asp Val 
1               5                   10                  15      


Val Trp Val Thr Leu Pro Ser Ala Glu Tyr Ala Gln Ser Gln Ser Ala 
            20                  25                  30          


Met Gly Ser His Pro Ala Tyr Trp Ser Glu Glu Ala Thr Ile Ile Asn 
        35                  40                  45              


Val Ala Thr Gly Gln Arg Ala Ala Val Ser Ser Ile Lys Trp Asp Gln 
    50                  55                  60                  


Val Thr Leu Asn Gly Lys Ala Leu His Lys Glu Thr His Ser Gly Leu 
65                  70                  75                  80  


Val Tyr Tyr Gln Leu Pro Leu Met Gly Lys Ile Asn Phe Trp Gln Gln 
                85                  90                  95      


Gly Thr Thr Lys Ala Gly Tyr Thr Tyr Asn Tyr Asn Thr Thr Asp Ser 
            100                 105                 110         


Asp Ser Leu Trp Val Trp Trp Asp Gly Gly Ser Lys Ala Tyr Leu Tyr 
        115                 120                 125             


Ile Ser Thr Tyr Thr Thr Met Leu Gly Ala Gly Pro Val Asn Ile Thr 
    130                 135                 140                 


Gly Leu Gly Ala Val Gly Pro 
145                 150     


<210>  32
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acids sequence of HEV-C MN450853

<400>  32

Ser Pro Ala Pro Ala Arg Pro Ile Thr Asn Trp Arg Ser Gly Asp Val 
1               5                   10                  15      


Val Trp Val Thr Leu Pro Ser Ala Glu Tyr Ala Gln Ser Gln Ser Ala 
            20                  25                  30          


Met Gly Ser His Pro Ala Tyr Trp Ser Glu Glu Ala Ser Ile Ile Asn 
        35                  40                  45              


Val Ala Thr Gly Gln Arg Ala Ala Val Ser Gly Ile Lys Trp Asp Gln 
    50                  55                  60                  


Val Ile Leu Asn Gly Arg Ala Leu His Lys Glu Thr His Ser Gly Leu 
65                  70                  75                  80  


Val Tyr Tyr Gln Leu Pro Leu Met Gly Lys Ile Ser Phe Trp Gln Gln 
                85                  90                  95      


Gly Thr Thr Lys Ala Gly Tyr Thr Tyr Asn Tyr Asp Thr Thr Asp Ser 
            100                 105                 110         


Asp Ser Leu Trp Val Trp Trp Asp Gly Gly Ser Lys Ala Tyr Leu Tyr 
        115                 120                 125             


Val Ser Thr Tyr Thr Ala Met Leu Gly Ala Gly Pro Val Asn Ile Thr 
    130                 135                 140                 


Gly Val Gly Ala Val Gly Pro 
145                 150     


<210>  33
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acids sequence of HEV-C MK050105

<400>  33

Ser Pro Ala Pro Ala Arg Pro Ile Thr Asn Trp Arg Ser Gly Asp Val 
1               5                   10                  15      


Val Trp Val Thr Leu Pro Ser Ala Glu Tyr Ala Gln Ser Gln Ser Ala 
            20                  25                  30          


Met Gly Ser His Pro Ala Tyr Trp Ser Glu Glu Ala Thr Ile Ile Asn 
        35                  40                  45              


Val Ala Thr Gly Gln Arg Ala Ser Val Ser Ser Ile Lys Trp Asp Gln 
    50                  55                  60                  


Val Thr Leu Asn Gly Lys Ala Leu His Lys Glu Thr His Ser Gly Leu 
65                  70                  75                  80  


Val Tyr Tyr Gln Leu Pro Leu Met Gly Lys Ile Ser Phe Trp Gln Gln 
                85                  90                  95      


Gly Thr Thr Lys Ala Gly Tyr Thr Tyr Asn Tyr Asn Thr Thr Asp Ser 
            100                 105                 110         


Asp Ser Leu Trp Val Trp Trp Asp Gly Ala Ser Lys Ala Tyr Leu Tyr 
        115                 120                 125             


Leu Ser Thr Tyr Thr Thr Met Leu Gly Ala Gly Pro Val Asn Ile Thr 
    130                 135                 140                 


Gly Leu Gly Ala Ile Gly Pro 
145                 150     


