                         SEQUENCE LISTING

<110>  Centro de Investigacin y de Estudios Avanzados del I.P.N.
 
<120>  Vacunas efectivas para la prevencin del botulismo

<130>  Cinvestav47

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  MISC_FEATURE
<223>  Antgeno C

<400>  1

Leu Pro Tyr Pro Asn Gly Leu Tyr Val Ile Ala Ala Ala Glu Tyr Tyr 
1               5                   10                  15      


Leu Pro Ser Leu Gly Lys Cys Glu Val Ser Asp Ala Pro Ser Pro Gln 
            20                  25                  30          


Ala Pro Val Val Glu Thr Ala Ala Ile Ile Val Gly Val Ile Asn Ala 
        35                  40                  45              


Ala Gln Asn Asn Ala Cys Tyr Val Leu Phe Lys Ala Ala Asn Leu Pro 
    50                  55                  60                  


Pro Leu Ile Ile Tyr Glu Ala Ala Lys Ser Tyr Leu Ile Val Ser Leu 
65                  70                  75                  80  


Ala Ala Tyr Ser Asp Leu Leu Asn Asn Val Glu Pro Ile Ser Leu Leu 
                85                  90                  95      


Asn Gly Ala Thr Tyr Ile Phe Arg Val Lys Val 
            100                 105         


<210>  2
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  misc_feature
<223>  DNA Antgeno C

<220>
<221>  misc_feature
<223>  Secuencia flanqueada con sitios NdeI/BamHI

<220>
<221>  misc_feature
<223>  Secuencia codificante para el Antgeno C

<220>
<221>  CDS
<222>  (1)..(336)

<400>  2
cat atg ctg ccg tac ccg aac ggt ctg tat gtg atc gcg gcg gcg gag         48
His Met Leu Pro Tyr Pro Asn Gly Leu Tyr Val Ile Ala Ala Ala Glu           
1               5                   10                  15                

tac tat ctg ccg agc ctg ggc aag tgc gaa gtt agc gat gcg ccg agc         96
Tyr Tyr Leu Pro Ser Leu Gly Lys Cys Glu Val Ser Asp Ala Pro Ser           
            20                  25                  30                    

ccg cag gcg ccg gtg gtt gag acc gcg gcg atc att gtg ggt gtt atc        144
Pro Gln Ala Pro Val Val Glu Thr Ala Ala Ile Ile Val Gly Val Ile           
        35                  40                  45                        

aac gcg gcg caa aac aac gcg tgc tac gtg ctg ttc aag gcg gcg aac        192
Asn Ala Ala Gln Asn Asn Ala Cys Tyr Val Leu Phe Lys Ala Ala Asn           
    50                  55                  60                            

ctg ccg ccg ctg atc att tac gag gcg gcg aaa agc tat ctg att gtg        240
Leu Pro Pro Leu Ile Ile Tyr Glu Ala Ala Lys Ser Tyr Leu Ile Val           
65                  70                  75                  80            

agc ctg gcg gcg tac agc gat ctg ctg aac aac gtt gaa ccg atc agc        288
Ser Leu Ala Ala Tyr Ser Asp Leu Leu Asn Asn Val Glu Pro Ile Ser           
                85                  90                  95                

ctg ctg aac ggc gcg acc tat att ttt cgt gtg aaa gtt taa gga tcc        336
Leu Leu Asn Gly Ala Thr Tyr Ile Phe Arg Val Lys Val     Gly Ser           
            100                 105                     110               


<210>  3
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  3

His Met Leu Pro Tyr Pro Asn Gly Leu Tyr Val Ile Ala Ala Ala Glu 
1               5                   10                  15      


Tyr Tyr Leu Pro Ser Leu Gly Lys Cys Glu Val Ser Asp Ala Pro Ser 
            20                  25                  30          


Pro Gln Ala Pro Val Val Glu Thr Ala Ala Ile Ile Val Gly Val Ile 
        35                  40                  45              


Asn Ala Ala Gln Asn Asn Ala Cys Tyr Val Leu Phe Lys Ala Ala Asn 
    50                  55                  60                  


Leu Pro Pro Leu Ile Ile Tyr Glu Ala Ala Lys Ser Tyr Leu Ile Val 
65                  70                  75                  80  


Ser Leu Ala Ala Tyr Ser Asp Leu Leu Asn Asn Val Glu Pro Ile Ser 
                85                  90                  95      


Leu Leu Asn Gly Ala Thr Tyr Ile Phe Arg Val Lys Val 
            100                 105                 


<210>  4
<211>  285
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  MISC_FEATURE
<223>  Antgeno D

<400>  4

Ile Thr Pro Ser Val Leu Ile Phe Gly Pro Leu Pro Asn Ile Leu Asp 
1               5                   10                  15      


Tyr Thr Ala Ser Leu Thr Leu Gln Ala Ala Gly Thr Leu Ser Ile Leu 
            20                  25                  30          


Lys Val Ala Pro Glu Phe Leu Leu Thr Phe Ser Asp Ala Ala Gln Ser 
        35                  40                  45              


Ser Ala Val Leu Gly Lys Ser Ile Phe Cys Met Asp Pro Val Ile Ala 
    50                  55                  60                  


Leu Met His Glu Leu Thr His Ser Leu His Gln Leu Tyr Gly Ile Ala 
65                  70                  75                  80  


Ala Lys Phe Asn Ser Leu Tyr Ser Asp Leu Thr Asn Val Met Ser Glu 
                85                  90                  95      


Val Val Tyr Ser Ser Gln Tyr Ala Ala Leu Gln Lys Leu Ser Ser Glu 
            100                 105                 110         


Ser Val Val Asp Leu Phe Thr Lys Val Cys Leu Arg Ala Ala Lys Asp 
        115                 120                 125             


Val Asp Tyr Leu Asn Ser Tyr Tyr Tyr Leu Glu Ala Ala Lys Ile Ser 
    130                 135                 140                 


Asp Val Ser Ala Ile Ile Pro Tyr Ile Gly Pro Ala Ala Lys Gln Ala 
145                 150                 155                 160 


Phe Ala Thr Ala Gly Val Ala Phe Leu Leu Glu Gly Ala Ala Pro Glu 
                165                 170                 175     


Phe Thr Ile Pro Ala Leu Gly Val Phe Thr Phe Tyr Ser Ser Ala Ala 
            180                 185                 190         


Phe Asn His Ile Ser Tyr Gln Met Tyr Asp Ser Leu Ser Tyr Gln Ala 
        195                 200                 205             


Asp Ala Ile Lys Ala Lys Ile Asp Leu Glu Tyr Ala Ala Ser Asp Ser 
    210                 215                 220                 


His Asn Ile Ile Leu Val Gly Glu Val Asp Arg Leu Lys Ala Ala Lys 
225                 230                 235                 240 


Tyr Gly Ser Phe Ile Ile Gln Pro Cys Asn Thr Phe Asp Tyr Tyr Ala 
                245                 250                 255     


Ser Gln Leu Phe Leu Ser Ala Ala Phe Asn His Glu Tyr Leu Ile Pro 
            260                 265                 270         


Val Ile Lys Ile Glu His Tyr Ala Ser Leu Leu Glu Ser 
        275                 280                 285 


<210>  5
<211>  870
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sintetico


<220>
<221>  misc_feature
<223>  DNA Antgeno D

<220>
<221>  misc_feature
<223>  Secuencia flanqueada con sitios NdeI/BamHI

<220>
<221>  misc_feature
<223>  Secuencia codificante para el Antgeno D

<220>
<221>  CDS
<222>  (1)..(870)

<400>  5
gga tcc atc acc ccg agc gtg ctg att ttc ggt ccg ctg ccg aac atc         48
Gly Ser Ile Thr Pro Ser Val Leu Ile Phe Gly Pro Leu Pro Asn Ile           
1               5                   10                  15                

ctg gac tat acc gcg agc ctg acc ctg cag gcg gcg ggt acc ctg agc         96
Leu Asp Tyr Thr Ala Ser Leu Thr Leu Gln Ala Ala Gly Thr Leu Ser           
            20                  25                  30                    

att ctg aag gtt gcg ccg gag ttc ctg ctg acc ttt agc gat gcg gcg        144
Ile Leu Lys Val Ala Pro Glu Phe Leu Leu Thr Phe Ser Asp Ala Ala           
        35                  40                  45                        

caa agc agc gcg gtg ctg ggt aaa agc atc ttc tgc atg gac ccg gtt        192
Gln Ser Ser Ala Val Leu Gly Lys Ser Ile Phe Cys Met Asp Pro Val           
    50                  55                  60                            

att gcg ctg atg cac gaa ctg acc cac agc ctg cac cag ctg tat ggc        240
Ile Ala Leu Met His Glu Leu Thr His Ser Leu His Gln Leu Tyr Gly           
65                  70                  75                  80            

atc gcg gcg aag ttt aac agc ctg tac agc gac ctg acc aac gtg atg        288
Ile Ala Ala Lys Phe Asn Ser Leu Tyr Ser Asp Leu Thr Asn Val Met           
                85                  90                  95                

agc gag gtg gtt tat agc agc cag tac gcg gcg ctg caa aaa ctg agc        336
Ser Glu Val Val Tyr Ser Ser Gln Tyr Ala Ala Leu Gln Lys Leu Ser           
            100                 105                 110                   

agc gaa agc gtg gtt gac ctg ttc acc aag gtg tgc ctg cgt gcg gcg        384
Ser Glu Ser Val Val Asp Leu Phe Thr Lys Val Cys Leu Arg Ala Ala           
        115                 120                 125                       

aaa gac gtt gat tat ctg aac agc tac tat tac ctg gag gcg gcg aag        432
Lys Asp Val Asp Tyr Leu Asn Ser Tyr Tyr Tyr Leu Glu Ala Ala Lys           
    130                 135                 140                           

atc agc gat gtt agc gcg atc att ccg tac att ggt ccg gcg gcg aaa        480
Ile Ser Asp Val Ser Ala Ile Ile Pro Tyr Ile Gly Pro Ala Ala Lys           
145                 150                 155                 160           

caa gcg ttt gcg acc gcg ggt gtg gcg ttt ctg ctg gaa ggt gct gcg        528
Gln Ala Phe Ala Thr Ala Gly Val Ala Phe Leu Leu Glu Gly Ala Ala           
                165                 170                 175               

ccg gaa ttc acc atc ccg gcg ctg ggc gtt ttc acc ttt tat agc agc        576
Pro Glu Phe Thr Ile Pro Ala Leu Gly Val Phe Thr Phe Tyr Ser Ser           
            180                 185                 190                   

gcg gcg ttt aac cac att agc tat cag atg tac gac agc ctg agc tat        624
Ala Ala Phe Asn His Ile Ser Tyr Gln Met Tyr Asp Ser Leu Ser Tyr           
        195                 200                 205                       

caa gcg gat gcg atc aag gcg aaa att gac ctg gag tac gcg gcg agc        672
Gln Ala Asp Ala Ile Lys Ala Lys Ile Asp Leu Glu Tyr Ala Ala Ser           
    210                 215                 220                           

gat agc cac aac atc att ctg gtg ggt gaa gtt gac cgt ctg aag gcg        720
Asp Ser His Asn Ile Ile Leu Val Gly Glu Val Asp Arg Leu Lys Ala           
225                 230                 235                 240           

gcg aaa tac ggc agc ttc atc att cag ccg tgc aac acc ttt gat tat        768
Ala Lys Tyr Gly Ser Phe Ile Ile Gln Pro Cys Asn Thr Phe Asp Tyr           
                245                 250                 255               

tac gcg agc caa ctg ttc ctg agc gcg gcg ttc aat cac gaa tat ctg        816
Tyr Ala Ser Gln Leu Phe Leu Ser Ala Ala Phe Asn His Glu Tyr Leu           
            260                 265                 270                   

atc ccg gtg atc aag att gag cac tac gcg agc ctg ctg gaa agc taa        864
Ile Pro Val Ile Lys Ile Glu His Tyr Ala Ser Leu Leu Glu Ser               
        275                 280                 285                       

gga tcc                                                                870
Gly Ser                                                                   
                                                                          


<210>  6
<211>  287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6

Gly Ser Ile Thr Pro Ser Val Leu Ile Phe Gly Pro Leu Pro Asn Ile 
1               5                   10                  15      


Leu Asp Tyr Thr Ala Ser Leu Thr Leu Gln Ala Ala Gly Thr Leu Ser 
            20                  25                  30          


Ile Leu Lys Val Ala Pro Glu Phe Leu Leu Thr Phe Ser Asp Ala Ala 
        35                  40                  45              


Gln Ser Ser Ala Val Leu Gly Lys Ser Ile Phe Cys Met Asp Pro Val 
    50                  55                  60                  


Ile Ala Leu Met His Glu Leu Thr His Ser Leu His Gln Leu Tyr Gly 
65                  70                  75                  80  


Ile Ala Ala Lys Phe Asn Ser Leu Tyr Ser Asp Leu Thr Asn Val Met 
                85                  90                  95      


Ser Glu Val Val Tyr Ser Ser Gln Tyr Ala Ala Leu Gln Lys Leu Ser 
            100                 105                 110         


Ser Glu Ser Val Val Asp Leu Phe Thr Lys Val Cys Leu Arg Ala Ala 
        115                 120                 125             


Lys Asp Val Asp Tyr Leu Asn Ser Tyr Tyr Tyr Leu Glu Ala Ala Lys 
    130                 135                 140                 


Ile Ser Asp Val Ser Ala Ile Ile Pro Tyr Ile Gly Pro Ala Ala Lys 
145                 150                 155                 160 


Gln Ala Phe Ala Thr Ala Gly Val Ala Phe Leu Leu Glu Gly Ala Ala 
                165                 170                 175     


Pro Glu Phe Thr Ile Pro Ala Leu Gly Val Phe Thr Phe Tyr Ser Ser 
            180                 185                 190         


Ala Ala Phe Asn His Ile Ser Tyr Gln Met Tyr Asp Ser Leu Ser Tyr 
        195                 200                 205             


Gln Ala Asp Ala Ile Lys Ala Lys Ile Asp Leu Glu Tyr Ala Ala Ser 
    210                 215                 220                 


Asp Ser His Asn Ile Ile Leu Val Gly Glu Val Asp Arg Leu Lys Ala 
225                 230                 235                 240 


Ala Lys Tyr Gly Ser Phe Ile Ile Gln Pro Cys Asn Thr Phe Asp Tyr 
                245                 250                 255     


Tyr Ala Ser Gln Leu Phe Leu Ser Ala Ala Phe Asn His Glu Tyr Leu 
            260                 265                 270         


Ile Pro Val Ile Lys Ile Glu His Tyr Ala Ser Leu Leu Glu Ser 
        275                 280                 285         


<210>  7
<211>  311
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sinttico


<220>
<221>  MISC_FEATURE
<223>  Antgeno D-His

<220>
<221>  MISC_FEATURE
<223>  Fragmento 1-24 bandera (tag) de histidinas

<400>  7

Met Gly His His His His His His His His His His Ser Ser Gly His 
1               5                   10                  15      


Ile Asp Asp Asp Asp Leu His Met Ile Thr Pro Ser Val Leu Ile Phe 
            20                  25                  30          


Gly Pro Leu Pro Asn Ile Leu Asp Tyr Thr Ala Ser Leu Thr Leu Gln 
        35                  40                  45              


Ala Ala Gly Thr Leu Ser Ile Leu Lys Val Ala Pro Glu Phe Leu Leu 
    50                  55                  60                  


Thr Phe Ser Asp Val Thr Ser Asn Gln Ser Ser Ala Val Leu Gly Lys 
65                  70                  75                  80  


Ser Ile Phe Cys Met Asp Pro Val Ile Ala Leu Met His Glu Leu Thr 
                85                  90                  95      


His Ser Leu His Gln Leu Tyr Gly Ile Ala Ala Lys Phe Asn Ser Leu 
            100                 105                 110         


Tyr Ser Asp Leu Thr Asn Val Met Ser Glu Val Val Tyr Ser Ser Gln 
        115                 120                 125             


Tyr Ala Ala Leu Gln Lys Ser Ser Glu Ser Val Val Asp Leu Phe Thr 
    130                 135                 140                 


Lys Val Cys Leu Arg Ala Ala Lys Asp Val Asp Tyr Leu Asn Asp Ser 
145                 150                 155                 160 


Tyr Tyr Tyr Leu Glu Ala Ala Lys Ile Ser Asp Val Ser Ala Ile Ile 
                165                 170                 175     


Pro Tyr Ile Gly Pro Ala Ala Lys Gln Ala Phe Ala Thr Ala Gly Val 
            180                 185                 190         


Ala Phe Leu Leu Glu Gly Ala Ala Pro Glu Phe Thr Ile Pro Ala Leu 
        195                 200                 205             


Gly Val Phe Thr Phe Tyr Ser Ser Ala Ala Phe Asn His Ile Ser Tyr 
    210                 215                 220                 


Gln Met Tyr Asp Ser Leu Ser Tyr Gln Ala Asp Ala Ile Lys Ala Lys 
225                 230                 235                 240 


Ile Asp Leu Glu Tyr Ala Ala Ser Asp Ser His Asn Ile Ile Leu Val 
                245                 250                 255     


Gly Glu Val Asp Arg Leu Lys Ala Ala Lys Tyr Gly Ser Phe Ile Ile 
            260                 265                 270         


Gln Pro Cys Asn Thr Phe Asp Tyr Tyr Ala Ser Gln Leu Phe Leu Ser 
        275                 280                 285             


Ala Ala Phe Asn His Glu Tyr Leu Ile Pro Val Ile Lys Ile Glu His 
    290                 295                 300                 


Tyr Ala Ser Leu Leu Glu Ser 
305                 310     


<210>  8
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 1 toxina C de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 73-82

<400>  8

Lys Ser Tyr Leu Ile Val Ser Leu Ala Ala 
1               5                   10  


<210>  9
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 2 toxina C de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 39-48

<400>  9

Ala Ala Ile Ile Val Gly Val Ile Asn Ala 
1               5                   10  


<210>  10
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 3 toxina C de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 18-27

<400>  10

Pro Ser Leu Gly Lys Cys Glu Val Ser Asp 
1               5                   10  


<210>  11
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 4 toxina C de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 98-107

<400>  11

Gly Ala Thr Tyr Ile Phe Arg Val Lys Val 
1               5                   10  


<210>  12
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 5 toxina C de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 61-70

<400>  12

Ala Asn Leu Pro Pro Leu Ile Ile Tyr Glu 
1               5                   10  


<210>  13
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 6 toxina C de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 4-13

<400>  13

Pro Asn Gly Leu Tyr Val Ile Ala Ala Ala 
1               5                   10  


<210>  14
<211>  44
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Dominio antignico 1 de la toxina C

<220>
<221>  MISC_FEATURE
<223>  Fragmento 4-47

<400>  14

Pro Asn Gly Leu Tyr Val Ile Ala Ala Ala Glu Tyr Tyr Leu Pro Ser 
1               5                   10                  15      


Leu Gly Lys Cys Glu Val Ser Asp Ala Pro Ser Pro Gln Ala Pro Val 
            20                  25                  30          


Val Glu Thr Ala Ala Ile Ile Val Gly Val Ile Asn 
        35                  40                  


<210>  15
<211>  54
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Dominio antignico 2 de la toxina C

<220>
<221>  MISC_FEATURE
<223>  Fragmento 50-103

<400>  15

Gln Asn Asn Ala Cys Tyr Val Leu Phe Lys Ala Ala Asn Leu Pro Pro 
1               5                   10                  15      


Leu Ile Ile Tyr Glu Ala Ala Lys Ser Tyr Leu Ile Val Ser Leu Ala 
            20                  25                  30          


Ala Tyr Ser Asp Leu Leu Asn Asn Val Glu Pro Ile Ser Leu Leu Asn 
        35                  40                  45              


Gly Ala Thr Tyr Ile Phe 
    50                  


<210>  16
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 1 toxina D de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 88-97

<400>  16

Ser Asp Leu Thr Asn Val Met Ser Glu Val 
1               5                   10  


<210>  17
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 2 toxina D de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 141-150

<400>  17

Ala Lys Ile Ser Asp Val Ser Ala Ile Ile 
1               5                   10  


<210>  18
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 3 toxina D de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 50-59

<400>  18

Ala Val Leu Gly Lys Ser Ile Phe Cys Met 
1               5                   10  


<210>  19
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 4 toxina D de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 26-35

<400>  19

Ala Gly Thr Leu Ser Ile Leu Lys Val Ala 
1               5                   10  


<210>  20
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 5 toxina D de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 224-233

<400>  20

Ser His Asn Ile Ile Leu Val Gly Glu Val 
1               5                   10  


<210>  21
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 6 toxina D de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 260-269

<400>  21

Phe Leu Ser Ala Ala Phe Asn His Glu Tyr 
1               5                   10  


<210>  22
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 7 toxina D de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 63-72

<400>  22

Ile Ala Leu Met His Glu Leu Thr His Ser 
1               5                   10  


<210>  23
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 8 toxina D de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 275-284

<400>  23

Lys Ile Glu His Tyr Ala Ser Leu Leu Glu 
1               5                   10  


<210>  24
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 9 toxina D de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 37-46

<400>  24

Glu Phe Leu Leu Thr Phe Ser Asp Ala Ala 
1               5                   10  


<210>  25
<211>  10
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Epitope 10 toxina D de Clostridium

<220>
<221>  MISC_FEATURE
<223>  Fragmento 112-121

<400>  25

Glu Ser Val Val Asp Leu Phe Thr Lys Val 
1               5                   10  


<210>  26
<211>  76
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Determinante antignico 1 de la toxina D

<220>
<221>  MISC_FEATURE
<223>  Fragmento 4-79

<400>  26

Ser Val Leu Ile Phe Gly Pro Leu Pro Asn Ile Leu Asp Tyr Thr Ala 
1               5                   10                  15      


Ser Leu Thr Leu Gln Ala Ala Gly Thr Leu Ser Ile Leu Lys Val Ala 
            20                  25                  30          


Pro Glu Phe Leu Leu Thr Phe Ser Asp Ala Ala Gln Ser Ser Ala Val 
        35                  40                  45              


Leu Gly Lys Ser Ile Phe Cys Met Asp Pro Val Ile Ala Leu Met His 
    50                  55                  60                  


Glu Leu Thr His Ser Leu His Gln Leu Tyr Gly Ile 
65                  70                  75      


<210>  27
<211>  60
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Determinante antignico 2 de la toxina D

<220>
<221>  MISC_FEATURE
<223>  Fragmento 81-140

<400>  27

Ala Lys Phe Asn Ser Leu Tyr Ser Asp Leu Thr Asn Val Met Ser Glu 
1               5                   10                  15      


Val Val Tyr Ser Ser Gln Tyr Ala Ala Leu Gln Lys Leu Ser Ser Glu 
            20                  25                  30          


Ser Val Val Asp Leu Phe Thr Lys Val Cys Leu Arg Ala Ala Lys Asp 
        35                  40                  45              


Val Asp Tyr Leu Asn Ser Tyr Tyr Tyr Leu Glu Ala 
    50                  55                  60  


<210>  28
<211>  31
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Determinante antignico 3 de la toxina D

<220>
<221>  MISC_FEATURE
<223>  Fragmento 142-172

<400>  28

Lys Ile Ser Asp Val Ser Ala Ile Ile Pro Tyr Ile Gly Pro Ala Ala 
1               5                   10                  15      


Lys Gln Ala Phe Ala Thr Ala Gly Val Ala Phe Leu Leu Glu Gly 
            20                  25                  30      


<210>  29
<211>  47
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Determinante antignico 4 de la toxina D

<220>
<221>  MISC_FEATURE
<223>  Fragmento 175-221

<400>  29

Pro Glu Phe Thr Ile Pro Ala Leu Gly Val Phe Thr Phe Tyr Ser Ser 
1               5                   10                  15      


Ala Ala Phe Asn His Ile Ser Tyr Gln Met Tyr Asp Ser Leu Ser Tyr 
            20                  25                  30          


Gln Ala Asp Ala Ile Lys Ala Lys Ile Asp Leu Glu Tyr Ala Ala 
        35                  40                  45          


<210>  30
<211>  12
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Determinante antignico 5 de la toxina D

<220>
<221>  MISC_FEATURE
<223>  Fragmento 224-235

<400>  30

Ser His Asn Ile Ile Leu Val Gly Glu Val Asp Arg 
1               5                   10          


<210>  31
<211>  27
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Determinante antignico 6 de la toxina D

<220>
<221>  MISC_FEATURE
<223>  Fragmento 237-263

<400>  31

Lys Ala Ala Lys Tyr Gly Ser Phe Ile Ile Gln Pro Cys Asn Thr Phe 
1               5                   10                  15      


Asp Tyr Tyr Ala Ser Gln Leu Phe Leu Ser Ala 
            20                  25          


<210>  32
<211>  16
<212>  PRT
<213>  Clostridium


<220>
<221>  MISC_FEATURE
<223>  Determinante antignico 7 de la toxina D

<220>
<221>  MISC_FEATURE
<223>  Fragmento 265-280

<400>  32

Phe Asn His Glu Tyr Leu Ile Pro Val Ile Lys Ile Glu His Tyr Ala 
1               5                   10                  15      


