                         SEQUENCE LISTING

<110>  ILLUMINA, INC.
 
<120>  IMPROVED METHODS OF ISOTHERMAL COMPLEMENTARY DNA AND LIBRARY 
       PREPARATION

<130>  01243-0026-00PCT

<150>  US 63/167,909
<151>  2021-03-30

<150>  US 63/234,114
<151>  2021-08-17

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mosaic end (ME) sequence (transferred strand)

<400>  1
agatgtgtat aagagacag                                                    19


<210>  2
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Outside end sequence

<400>  2
ctgactctta tacacaagt                                                    19


<210>  3
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Inside end sequence

<400>  3
ctgtctcttg atcagatct                                                    19


<210>  4
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mosaic end (non-transferred strand)

<400>  4
ctgtctctta tacacatct                                                    19


<210>  5
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  U16 transferred strand (TS), Modified ME with A16U substitution 
       (transferred strand)

<400>  5
agatgtgtat aagagucag                                                    19


<210>  6
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Modified ME (non-transferred strand) with T16A substitution

<400>  6
ctgactctta tacacatct                                                    19


<210>  7
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  U17 TS, Modified ME with C17U substitution (transferred strand)

<400>  7
agatgtgtat aagagauag                                                    19


<210>  8
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Modified ME (non-transferred strand) with G17A substitution

<400>  8
ctatctctta tacacatct                                                    19


<210>  9
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  U18 TS, Modified ME with A18U substitution (transferred strand)

<400>  9
agatgtgtat aagagacug                                                    19


<210>  10
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Modified ME (non-transferred strand) with T18A substitution

<400>  10
cagtctctta tacacatct                                                    19


<210>  11
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  A14 primer sequence

<400>  11
tcgtcggcag cgtc                                                         14


<210>  12
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  B15 primer sequence

<400>  12
gtctcgtggg ctcgg                                                        15


<210>  13
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  P5 primer sequence

<400>  13
aatgatacgg cgaccaccga gauctacac                                         29


<210>  14
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  P7 primer sequence

<400>  14
caagcagaag acggcatacg agat                                              24


<210>  15
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Biotinylated ME


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5' phosphate

<220>
<221>  modified_base
<222>  (19)..(19)
<223>  3 biotin

<400>  15
ctgtctctta tacacatct                                                    19


<210>  16
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  I19 TS, Modified ME with G19I substitution


<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  n is inosine

<400>  16
agatgtgtat aagagacan                                                    19


<210>  17
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  U19 TS Modified ME with G19U substitution

<400>  17
agatgtgtat aagagacau                                                    19


<210>  18
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  O16 TS, Modified ME with A16O substitution


<220>
<221>  modified_base
<222>  (16)..(16)
<223>  8-oxo-guanine

<400>  18
agatgtgtat aagaggcag                                                    19


<210>  19
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  O17 TS, Modified ME with C17O substitution


<220>
<221>  modified_base
<222>  (17)..(17)
<223>  8-oxo-guanine

<400>  19
agatgtgtat aagagagag                                                    19


<210>  20
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  O18 TS, Modified ME with A18O substitution


<220>
<221>  modified_base
<222>  (18)..(18)
<223>  8-oxo-guanine

<400>  20
agatgtgtat aagagacgg                                                    19


<210>  21
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  O19 TS Modified ME with G19O substitution


<220>
<221>  modified_base
<222>  (19)..(19)
<223>  8-oxo-guanine

<400>  21
agatgtgtat aagagacag                                                    19


<210>  22
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  I16 TS, Modified ME with A16I substitution


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is inosine

<400>  22
agatgtgtat aagagncag                                                    19


<210>  23
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  I17 TS, Modified ME with C17I substitution


<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  n is inosine

<400>  23
agatgtgtat aagaganag                                                    19


<210>  24
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  I18 TS, Modified ME with A18I substitution


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is inosine

<400>  24
agatgtgtat aagagacng                                                    19


