                         SEQUENCE LISTING

<110>  Century Therapeutics, Inc.
 
<120>  COMPOSITIONS AND METHODS FOR GENERATING ALPHA-BETA T CELLS FROM 
       INDUCED PLURIPOTENT STEM CELLS

<130>  CNTY-005-WO-01

<150>  63/171,650
<151>  2021-04-07

<160>  100   

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro 
            20          


<210>  2
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FMC63 VH

<400>  2

Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
            20                  25                  30          


Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  


Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  


Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala 
                85                  90                  95      


Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser 
        115                 120 


<210>  3
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Whitlow Linker

<400>  3

Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      


Lys Gly 
        


<210>  4
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FMC63 VL

<400>  4

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
            100                 105         


<210>  5
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  5

Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
1               5                   10                  15      


Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
            20                  25                  30          


Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly 
        35                  40                  45              


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
    50                  55                  60                  


Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
65                  70                  75                  80  


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
                85                  90                  95      


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
            100                 105         


<210>  6
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  6

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  7
<211>  245
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FMC63 scFV

<400>  7

Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
            20                  25                  30          


Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  


Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  


Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala 
                85                  90                  95      


Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser Gly Ser Thr Ser Gly Ser Gly Lys 
        115                 120                 125             


Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Asp Ile Gln Met Thr Gln 
    130                 135                 140                 


Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser 
145                 150                 155                 160 


Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln 
                165                 170                 175     


Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu 
            180                 185                 190         


His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
        195                 200                 205             


Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr 
    210                 215                 220                 


Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr 
225                 230                 235                 240 


Lys Leu Glu Ile Thr 
                245 


<210>  8
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  8

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  9
<211>  94
<212>  PRT
<213>  Homo sapiens

<400>  9

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Pro Leu Asn Thr Asp Ala Tyr Leu 
65                  70                  75                  80  


Ser Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
                85                  90                  


<210>  10
<211>  62
<212>  PRT
<213>  Homo sapiens

<400>  10

Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala Pro His Pro Lys Gln 
1               5                   10                  15      


Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu Pro Gly Ser Asn Thr 
            20                  25                  30          


Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys Gln Pro Val Thr Gln 
        35                  40                  45              


Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln Glu Arg Gln 
    50                  55                  60          


<210>  11
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  11

Ala Leu Tyr Leu Leu Arg Arg Asp Gln Arg Leu Pro Pro Asp Ala His 
1               5                   10                  15      


Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln 
            20                  25                  30          


Ala Asp Ala His Ser Thr Leu Ala Lys Ile 
        35                  40          


<210>  12
<211>  25
<212>  PRT
<213>  Homo sapiens

<400>  12

Thr Tyr Cys Phe Ala Pro Arg Cys Arg Glu Arg Arg Arg Asn Glu Arg 
1               5                   10                  15      


Leu Arg Arg Glu Ser Val Arg Pro Val 
            20                  25  


<210>  13
<211>  61
<212>  PRT
<213>  Homo sapiens

<400>  13

Lys Trp Lys Lys Lys Lys Arg Pro Arg Asn Ser Tyr Lys Cys Gly Thr 
1               5                   10                  15      


Asn Thr Met Glu Arg Glu Glu Ser Glu Gln Thr Lys Lys Arg Glu Lys 
            20                  25                  30          


Ile His Ile Pro Glu Arg Ser Asp Glu Ala Gln Arg Val Phe Lys Ser 
        35                  40                  45              


Ser Lys Thr Ser Ser Cys Asp Lys Ser Asp Thr Cys Phe 
    50                  55                  60      


<210>  14
<211>  48
<212>  PRT
<213>  Homo sapiens

<400>  14

Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro Val Glu Pro 
1               5                   10                  15      


Ala Glu Pro Cys His Tyr Ser Cys Pro Arg Glu Glu Glu Gly Ser Thr 
            20                  25                  30          


Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala Cys Ser Pro 
        35                  40                  45              


<210>  15
<211>  38
<212>  PRT
<213>  Homo sapiens

<400>  15

Cys Trp Leu Thr Lys Lys Lys Tyr Ser Ser Ser Val His Asp Pro Asn 
1               5                   10                  15      


Gly Glu Tyr Met Phe Met Arg Ala Val Asn Thr Ala Lys Lys Ser Arg 
            20                  25                  30          


Leu Thr Asp Val Thr Leu 
        35              


<210>  16
<211>  51
<212>  PRT
<213>  Homo sapiens

<400>  16

Met Gly Trp Ile Arg Gly Arg Arg Ser Arg His Ser Trp Glu Met Ser 
1               5                   10                  15      


Glu Phe His Asn Tyr Asn Leu Asp Leu Lys Lys Ser Asp Phe Ser Thr 
            20                  25                  30          


Arg Trp Gln Lys Gln Arg Cys Pro Val Val Lys Ser Lys Cys Arg Glu 
        35                  40                  45              


Asn Ala Ser 
    50      


<210>  17
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  17

Leu Cys Ala Arg Pro Arg Arg Ser Pro Ala Gln Glu Asp Gly Lys Val 
1               5                   10                  15      


Tyr Ile Asn Met Pro Gly Arg Gly 
            20                  


<210>  18
<211>  52
<212>  PRT
<213>  Homo sapiens

<400>  18

Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala 
1               5                   10                  15      


Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu Ser Pro Tyr Gln Glu 
            20                  25                  30          


Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp Leu Asn Thr Gln Arg 
        35                  40                  45              


Pro Tyr Tyr Lys 
    50          


<210>  19
<211>  120
<212>  PRT
<213>  Homo sapiens

<400>  19

Trp Arg Arg Lys Arg Lys Glu Lys Gln Ser Glu Thr Ser Pro Lys Glu 
1               5                   10                  15      


Phe Leu Thr Ile Tyr Glu Asp Val Lys Asp Leu Lys Thr Arg Arg Asn 
            20                  25                  30          


His Glu Gln Glu Gln Thr Phe Pro Gly Gly Gly Ser Thr Ile Tyr Ser 
        35                  40                  45              


Met Ile Gln Ser Gln Ser Ser Ala Pro Thr Ser Gln Glu Pro Ala Tyr 
    50                  55                  60                  


Thr Leu Tyr Ser Leu Ile Gln Pro Ser Arg Lys Ser Gly Ser Arg Lys 
65                  70                  75                  80  


Arg Asn His Ser Pro Ser Phe Asn Ser Thr Ile Tyr Glu Val Ile Gly 
                85                  90                  95      


Lys Ser Gln Pro Lys Ala Gln Asn Pro Ala Arg Leu Ser Arg Lys Glu 
            100                 105                 110         


Leu Glu Asn Phe Asp Val Tyr Ser 
        115                 120 


<210>  20
<211>  41
<212>  PRT
<213>  Homo sapiens

<400>  20

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  21
<211>  47
<212>  PRT
<213>  Homo sapiens

<400>  21

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr 
        35                  40                  45          


<210>  22
<211>  39
<212>  PRT
<213>  Homo sapiens

<400>  22

Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
1               5                   10                  15      


Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
            20                  25                  30          


Phe Pro Gly Pro Ser Lys Pro 
        35                  


<210>  23
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  23

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr 
            20      


<210>  24
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  24

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  25
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (G4S)3

<400>  25

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  26
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 3

<400>  26

Gly Gly Ser Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Ser 
1               5                   10                  15      


Thr Gly Gly Ser 
            20  


<210>  27
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 4

<400>  27

Gly Gly Gly Ser Gly Gly Gly Ser 
1               5               


<210>  28
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 5

<400>  28

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10          


<210>  29
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 6

<400>  29

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


<210>  30
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 7

<400>  30

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  31
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 8

<400>  31

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  32
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 9

<400>  32

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25  


<210>  33
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 10

<400>  33

Ile Arg Pro Arg Ala Ile Gly Gly Ser Lys Pro Arg Val Ala 
1               5                   10                  


<210>  34
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 11

<400>  34

Gly Lys Gly Gly Ser Gly Lys Gly Gly Ser Gly Lys Gly Gly Ser 
1               5                   10                  15  


<210>  35
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 12

<400>  35

Gly Gly Lys Gly Ser Gly Gly Lys Gly Ser Gly Gly Lys Gly Ser 
1               5                   10                  15  


<210>  36
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 13

<400>  36

Gly Gly Gly Lys Ser Gly Gly Gly Lys Ser Gly Gly Gly Lys Ser 
1               5                   10                  15  


<210>  37
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 14

<400>  37

Gly Lys Gly Lys Ser Gly Lys Gly Lys Ser Gly Lys Gly Lys Ser 
1               5                   10                  15  


<210>  38
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 15

<400>  38

Gly Gly Gly Lys Ser Gly Gly Lys Gly Ser Gly Lys Gly Gly Ser 
1               5                   10                  15  


<210>  39
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 16

<400>  39

Gly Lys Pro Gly Ser Gly Lys Pro Gly Ser Gly Lys Pro Gly Ser 
1               5                   10                  15  


<210>  40
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 17

<400>  40

Gly Lys Pro Gly Ser Gly Lys Pro Gly Ser Gly Lys Pro Gly Ser Gly 
1               5                   10                  15      


Lys Pro Gly Ser 
            20  


<210>  41
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 18

<400>  41

Gly Lys Gly Lys Ser Gly Lys Gly Lys Ser Gly Lys Gly Lys Ser Gly 
1               5                   10                  15      


Lys Gly Lys Ser 
            20  


<210>  42
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 19

<400>  42

Ser Thr Ala Gly Asp Thr His Leu Gly Gly Glu Asp Phe Asp 
1               5                   10                  


<210>  43
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 20

<400>  43

Gly Glu Gly Gly Ser Gly Glu Gly Gly Ser Gly Glu Gly Gly Ser 
1               5                   10                  15  


<210>  44
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 21

<400>  44

Gly Gly Glu Gly Ser Gly Gly Glu Gly Ser Gly Gly Glu Gly Ser 
1               5                   10                  15  


<210>  45
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 22

<400>  45

Gly Glu Gly Glu Ser Gly Glu Gly Glu Ser Gly Glu Gly Glu Ser 
1               5                   10                  15  


<210>  46
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 23

<400>  46

Gly Gly Gly Glu Ser Gly Gly Glu Gly Ser Gly Glu Gly Gly Ser 
1               5                   10                  15  


<210>  47
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 24

<400>  47

Gly Glu Gly Glu Ser Gly Glu Gly Glu Ser Gly Glu Gly Glu Ser Gly 
1               5                   10                  15      


Glu Gly Glu Ser 
            20  


<210>  48
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 25

<400>  48

Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      


Lys Gly 
        


<210>  49
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 26

<400>  49

Pro Arg Gly Ala Ser Lys Ser Gly Ser Ala Ser Gln Thr Gly Ser Ala 
1               5                   10                  15      


Pro Gly Ser 
            


<210>  50
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 27

<400>  50

Gly Thr Ala Ala Ala Gly Ala Gly Ala Ala Gly Gly Ala Ala Ala Gly 
1               5                   10                  15      


Ala Ala Gly 
            


<210>  51
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 28

<400>  51

Gly Thr Ser Gly Ser Ser Gly Ser Gly Ser Gly Gly Ser Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly 
            


<210>  52
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 29

<400>  52

Gly Lys Pro Gly Ser Gly Lys Pro Gly Ser Gly Lys Pro Gly Ser Gly 
1               5                   10                  15      


Lys Pro Gly Ser 
            20  


<210>  53
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 30

<400>  53

Gly Ser Gly Ser 
1               


<210>  54
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 31

<400>  54

Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro 
1               5                   10  


<210>  55
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 32

<400>  55

Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro 
1               5                   10                  15      


Ala Pro Ala Pro 
            20  


<210>  56
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker 33

<400>  56

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Ala 
1               5                   10                  15      


Lys Glu Ala Ala Ala Ala Lys Glu Ala Ala Ala Ala Lys Ala Ala Ala 
            20                  25                  30          


<210>  57
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  57

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  58
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  58

Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro 
1               5                   10          


<210>  59
<211>  62
<212>  PRT
<213>  Homo sapiens

<400>  59

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro Arg Cys 
1               5                   10                  15      


Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro 
            20                  25                  30          


Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Glu 
        35                  40                  45              


Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro 
    50                  55                  60          


<210>  60
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  60

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro 
1               5                   10          


<210>  61
<211>  486
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 scFv CAR

<400>  61

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
            100                 105                 110         


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser 
    130                 135                 140                 


Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala 
145                 150                 155                 160 


Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu 
                165                 170                 175     


Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu 
            180                 185                 190         


Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser 
        195                 200                 205             


Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln 
    210                 215                 220                 


Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 
225                 230                 235                 240 


Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ile Glu Val Met Tyr 
            260                 265                 270         


Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile His 
        275                 280                 285             


Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser 
    290                 295                 300                 


Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr 
305                 310                 315                 320 


Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys 
                325                 330                 335     


Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg 
            340                 345                 350         


Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp 
        355                 360                 365             


Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
    370                 375                 380                 


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
385                 390                 395                 400 


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
                405                 410                 415     


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
            420                 425                 430         


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
        435                 440                 445             


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
    450                 455                 460                 


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
465                 470                 475                 480 


Gln Ala Leu Pro Pro Arg 
                485     


<210>  62
<211>  1461
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-CD19 scFv CAR

<400>  62
atgctgctgc tggtcacatc tctgctgctg tgcgagctgc cccatcctgc ctttctgctg       60

atccccgaca tccagatgac ccagaccaca agcagcctgt ctgccagcct gggcgataga      120

gtgaccatca gctgtagagc cagccaggac atcagcaagt acctgaactg gtatcagcaa      180

aagcccgacg gcaccgtgaa gctgctgatc taccacacca gcagactgca cagcggcgtg      240

ccaagcagat tttctggcag cggctctggc accgactaca gcctgacaat cagcaacctg      300

gaacaagagg atatcgctac ctacttctgc cagcaaggca acaccctgcc ttacaccttt      360

ggcggaggca ccaagctgga aatcaccggc tctacaagcg gcagcggcaa acctggatct      420

ggcgagggat ctaccaaggg cgaagtgaaa ctgcaagagt ctggccctgg actggtggcc      480

ccatctcagt ctctgagcgt gacctgtaca gtcagcggag tgtccctgcc tgattacggc      540

gtgtcctgga tcagacagcc tcctcggaaa ggcctggaat ggctgggagt gatctggggc      600

agcgagacaa cctactacaa cagcgccctg aagtcccggc tgaccatcat caaggacaac      660

tccaagagcc aggtgttcct gaagatgaac agcctgcaga ccgacgacac cgccatctac      720

tattgcgcca agcactacta ctacggcggc agctacgcca tggattattg gggccagggc      780

accagcgtga ccgtgtctag catcgaagtg atgtaccctc caccttacct ggacaacgag      840

aagtccaacg gcaccatcat ccacgtgaag ggcaagcacc tgtgtccttc tccactgttc      900

cccggaccta gcaagccttt ctgggtgctc gttgttgttg gcggcgtgct ggcctgttac      960

agcctgctgg ttaccgtggc cttcatcatc ttttgggtcc gaagcaagcg gagccggctg     1020

ctgcactccg actacatgaa catgacccct agacggcccg gaccaaccag aaagcactac     1080

cagccttacg ctcctcctag agacttcgcc gcctaccggt ccagagtgaa gttcagcaga     1140

tccgccgatg ctcccgccta tcagcagggc caaaaccagc tgtacaacga gctgaacctg     1200

gggagaagag aagagtacga cgtgctggac aagcggagag gcagagatcc tgaaatgggc     1260

ggcaagccca gacggaagaa tcctcaagag ggcctgtata atgagctgca gaaagacaag     1320

atggccgagg cctacagcga gatcggaatg aagggcgagc gcagaagagg caagggacac     1380

gatggactgt accagggact gagcaccgcc accaaggata cctatgacgc cctgcacatg     1440

caggccctgc ctccaagatg a                                               1461


<210>  63
<211>  1724
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAG Promoter

<400>  63
attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat       60

atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg      120

acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt      180

tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca gtacatcaag      240

tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc      300

attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag      360

tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct ccccatctcc      420

cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg      480

ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg      540

ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt      600

tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt      660

cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct      720

ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg      780

taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg aaagccttaa      840

agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg      900

tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg      960

cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc     1020

ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg     1080

ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca cccccctccc     1140

cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg tggcgcgggg     1200

ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct     1260

cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc ggctgtcgag     1320

gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc     1380

ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg     1440

gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg     1500

tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag ggggacggct     1560

gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcgggatat     1620

ctacgaagcg gccgccctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc     1680

tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaa                      1724


<210>  64
<211>  121
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SV40 terminator/polyadenylation

<400>  64
aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca       60

aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct      120

t                                                                      121


<210>  65
<211>  335
<212>  PRT
<213>  Homo sapiens

<400>  65

His Ser Leu Lys Tyr Phe His Thr Ser Val Ser Arg Pro Gly Arg Gly 
1               5                   10                  15      


Glu Pro Arg Phe Ile Ser Val Gly Tyr Val Asp Asp Thr Gln Phe Val 
            20                  25                  30          


Arg Phe Asp Asn Asp Ala Ala Ser Pro Arg Met Val Pro Arg Ala Pro 
        35                  40                  45              


Trp Met Glu Gln Glu Gly Ser Glu Tyr Trp Asp Arg Glu Thr Arg Ser 
    50                  55                  60                  


Ala Arg Asp Thr Ala Gln Ile Phe Arg Val Asn Leu Arg Thr Leu Arg 
65                  70                  75                  80  


Gly Tyr Tyr Asn Gln Ser Glu Ala Gly Ser His Thr Leu Gln Trp Met 
                85                  90                  95      


His Gly Cys Glu Leu Gly Pro Asp Gly Arg Phe Leu Arg Gly Tyr Glu 
            100                 105                 110         


Gln Phe Ala Tyr Asp Gly Lys Asp Tyr Leu Thr Leu Asn Glu Asp Leu 
        115                 120                 125             


Arg Ser Trp Thr Ala Val Asp Thr Ala Ala Gln Ile Ser Glu Gln Lys 
    130                 135                 140                 


Ser Asn Asp Ala Ser Glu Ala Glu His Gln Arg Ala Tyr Leu Glu Asp 
145                 150                 155                 160 


Thr Cys Val Glu Trp Leu His Lys Tyr Leu Glu Lys Gly Lys Glu Thr 
                165                 170                 175     


Leu Leu His Leu Glu Pro Pro Lys Thr His Val Thr His His Pro Ile 
            180                 185                 190         


Ser Asp His Glu Ala Thr Leu Arg Cys Trp Ala Leu Gly Phe Tyr Pro 
        195                 200                 205             


Ala Glu Ile Thr Leu Thr Trp Gln Gln Asp Gly Glu Gly His Thr Gln 
    210                 215                 220                 


Asp Thr Glu Leu Val Glu Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln 
225                 230                 235                 240 


Lys Trp Ala Ala Val Val Val Pro Ser Gly Glu Glu Gln Arg Tyr Thr 
                245                 250                 255     


Cys His Val Gln His Glu Gly Leu Pro Glu Pro Val Thr Leu Arg Trp 
            260                 265                 270         


Lys Pro Ala Ser Gln Pro Thr Ile Pro Ile Val Gly Ile Ile Ala Gly 
        275                 280                 285             


Leu Val Leu Leu Gly Ser Val Val Ser Gly Ala Val Val Ala Ala Val 
    290                 295                 300                 


Ile Trp Arg Lys Lys Ser Ser Gly Gly Lys Gly Gly Ser Tyr Ser Lys 
305                 310                 315                 320 


Ala Glu Trp Ser Asp Ser Ala Gln Gly Ser Glu Ser His Ser Leu 
                325                 330                 335 


<210>  66
<211>  504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA-G signal peptide-B2M-HLA-E

<400>  66

Met Val Val Met Ala Pro Arg Thr Leu Phe Leu Leu Leu Ser Gly Ala 
1               5                   10                  15      


Leu Thr Leu Thr Glu Thr Trp Ala Val Met Ala Pro Arg Thr Leu Ile 
            20                  25                  30          


Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        35                  40                  45              


Gly Gly Gly Gly Ser Ile Gln Arg Thr Pro Lys Ile Gln Val Tyr Ser 
    50                  55                  60                  


Arg His Pro Ala Glu Asn Gly Lys Ser Asn Phe Leu Asn Cys Tyr Val 
65                  70                  75                  80  


Ser Gly Phe His Pro Ser Asp Ile Glu Val Asp Leu Leu Lys Asn Gly 
                85                  90                  95      


Glu Arg Ile Glu Lys Val Glu His Ser Asp Leu Ser Phe Ser Lys Asp 
            100                 105                 110         


Trp Ser Phe Tyr Leu Leu Tyr Tyr Thr Glu Phe Thr Pro Thr Glu Lys 
        115                 120                 125             


Asp Glu Tyr Ala Cys Arg Val Asn His Val Thr Leu Ser Gln Pro Lys 
    130                 135                 140                 


Ile Val Lys Trp Asp Arg Asp Met Gly Gly Gly Gly Ser Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Gly Gly Gly Gly Ser Gly Ser His Ser Leu Lys Tyr Phe His 
                165                 170                 175     


Thr Ser Val Ser Arg Pro Gly Arg Gly Glu Pro Arg Phe Ile Ser Val 
            180                 185                 190         


Gly Tyr Val Asp Asp Thr Gln Phe Val Arg Phe Asp Asn Asp Ala Ala 
        195                 200                 205             


Ser Pro Arg Met Val Pro Arg Ala Pro Trp Met Glu Gln Glu Gly Ser 
    210                 215                 220                 


Glu Tyr Trp Asp Arg Glu Thr Arg Ser Ala Arg Asp Thr Ala Gln Ile 
225                 230                 235                 240 


Phe Arg Val Asn Leu Arg Thr Leu Arg Gly Tyr Tyr Asn Gln Ser Glu 
                245                 250                 255     


Ala Gly Ser His Thr Leu Gln Trp Met His Gly Cys Glu Leu Gly Pro 
            260                 265                 270         


Asp Gly Arg Phe Leu Arg Gly Tyr Glu Gln Phe Ala Tyr Asp Gly Lys 
        275                 280                 285             


Asp Tyr Leu Thr Leu Asn Glu Asp Leu Arg Ser Trp Thr Ala Val Asp 
    290                 295                 300                 


Thr Ala Ala Gln Ile Ser Glu Gln Lys Ser Asn Asp Ala Ser Glu Ala 
305                 310                 315                 320 


Glu His Gln Arg Ala Tyr Leu Glu Asp Thr Cys Val Glu Trp Leu His 
                325                 330                 335     


Lys Tyr Leu Glu Lys Gly Lys Glu Thr Leu Leu His Leu Glu Pro Pro 
            340                 345                 350         


Lys Thr His Val Thr His His Pro Ile Ser Asp His Glu Ala Thr Leu 
        355                 360                 365             


Arg Cys Trp Ala Leu Gly Phe Tyr Pro Ala Glu Ile Thr Leu Thr Trp 
    370                 375                 380                 


Gln Gln Asp Gly Glu Gly His Thr Gln Asp Thr Glu Leu Val Glu Thr 
385                 390                 395                 400 


Arg Pro Ala Gly Asp Gly Thr Phe Gln Lys Trp Ala Ala Val Val Val 
                405                 410                 415     


Pro Ser Gly Glu Glu Gln Arg Tyr Thr Cys His Val Gln His Glu Gly 
            420                 425                 430         


Leu Pro Glu Pro Val Thr Leu Arg Trp Lys Pro Ala Ser Gln Pro Thr 
        435                 440                 445             


Ile Pro Ile Val Gly Ile Ile Ala Gly Leu Val Leu Leu Gly Ser Val 
    450                 455                 460                 


Val Ser Gly Ala Val Val Ala Ala Val Ile Trp Arg Lys Lys Ser Ser 
465                 470                 475                 480 


Gly Gly Lys Gly Gly Ser Tyr Ser Lys Ala Glu Trp Ser Asp Ser Ala 
                485                 490                 495     


Gln Gly Ser Glu Ser His Ser Leu 
            500                 


<210>  67
<211>  1515
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-G signal peptide-B2M-HLA-E

<400>  67
atggtggtca tggcccctag aacactgttc ctgctgctgt ctggcgccct gacactgaca       60

gagacatggg ccgtgatggc ccccagaacc ctgatcctgg gcggcggtgg ttcaggcgga      120

ggaggttcag gaggaggggg tagtggaggt ggtggttcta tccagcggac ccctaagatc      180

caggtgtaca gcagacaccc cgccgagaac ggcaagagca acttcctgaa ctgctacgtg      240

tccggctttc accccagcga cattgaggtg gacctgctga agaacggcga gcggatcgag      300

aaggtggaac acagcgatct gagcttcagc aaggactggt ccttctacct gctgtactac      360

accgagttca cccctaccga gaaggacgag tacgcctgca gagtgaacca cgtgacactg      420

agccagccta agatcgtgaa gtgggatcgc gatatgggcg gaggcggatc tggtggcgga      480

ggaagtggcg gcggaggatc tggctcccac tccttgaagt atttccacac ttccgtgtcc      540

cggcccggcc gcggggagcc ccgcttcatc tctgtgggct acgtggacga cacccagttc      600

gtgcgcttcg acaacgacgc cgcgagtccg aggatggtgc cgcgggcgcc gtggatggag      660

caggaggggt cagagtattg ggaccgggag acacggagcg ccagggacac cgcacagatt      720

ttccgagtga atctgcggac gctgcgcggc tactacaatc agagcgaggc cgggtctcac      780

accctgcagt ggatgcatgg ctgcgagctg gggcccgacg ggcgcttcct ccgcgggtat      840

gaacagttcg cctacgacgg caaggattat ctcaccctga atgaggacct gcgctcctgg      900

accgcggtgg acacggcggc tcagatctcc gagcaaaagt caaatgatgc ctctgaggcg      960

gagcaccaga gagcctacct ggaagacaca tgcgtggagt ggctccacaa atacctggag     1020

aaggggaagg agacgctgct tcacctggag cccccaaaga cacacgtgac tcaccacccc     1080

atctctgacc atgaggccac cctgaggtgc tgggccctgg gcttctaccc tgcggagatc     1140

acactgacct ggcagcagga tggggagggc catacccagg acacggagct cgtggagacc     1200

aggcctgcag gggatggaac cttccagaag tgggcagctg tggtggtgcc ttctggagag     1260

gagcagagat acacgtgcca tgtgcagcat gaggggctac ccgagcccgt caccctgaga     1320

tggaagccgg cttcccagcc caccatcccc atcgtgggca tcattgctgg cctggttctc     1380

cttggatctg tggtctctgg agctgtggtt gctgctgtga tatggaggaa gaagagctca     1440

ggtggaaaag gagggagcta ctctaaggct gagtggagcg acagtgccca ggggtctgag     1500

tctcacagct tgtaa                                                      1515


<210>  68
<211>  312
<212>  PRT
<213>  Homo sapiens

<400>  68

His Ser Met Arg Tyr Phe Ser Ala Ala Val Ser Arg Pro Gly Arg Gly 
1               5                   10                  15      


Glu Pro Arg Phe Ile Ala Met Gly Tyr Val Asp Asp Thr Gln Phe Val 
            20                  25                  30          


Arg Phe Asp Ser Asp Ser Ala Cys Pro Arg Met Glu Pro Arg Ala Pro 
        35                  40                  45              


Trp Val Glu Gln Glu Gly Pro Glu Tyr Trp Glu Glu Glu Thr Arg Asn 
    50                  55                  60                  


Thr Lys Ala His Ala Gln Thr Asp Arg Met Asn Leu Gln Thr Leu Arg 
65                  70                  75                  80  


Gly Tyr Tyr Asn Gln Ser Glu Ala Ser Ser His Thr Leu Gln Trp Met 
                85                  90                  95      


Ile Gly Cys Asp Leu Gly Ser Asp Gly Arg Leu Leu Arg Gly Tyr Glu 
            100                 105                 110         


Gln Tyr Ala Tyr Asp Gly Lys Asp Tyr Leu Ala Leu Asn Glu Asp Leu 
        115                 120                 125             


Arg Ser Trp Thr Ala Ala Asp Thr Ala Ala Gln Ile Ser Lys Arg Lys 
    130                 135                 140                 


Cys Glu Ala Ala Asn Val Ala Glu Gln Arg Arg Ala Tyr Leu Glu Gly 
145                 150                 155                 160 


Thr Cys Val Glu Trp Leu His Arg Tyr Leu Glu Asn Gly Lys Glu Met 
                165                 170                 175     


Leu Gln Arg Ala Asp Pro Pro Lys Thr His Val Thr His His Pro Val 
            180                 185                 190         


Phe Asp Tyr Glu Ala Thr Leu Arg Cys Trp Ala Leu Gly Phe Tyr Pro 
        195                 200                 205             


Ala Glu Ile Ile Leu Thr Trp Gln Arg Asp Gly Glu Asp Gln Thr Gln 
    210                 215                 220                 


Asp Val Glu Leu Val Glu Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln 
225                 230                 235                 240 


Lys Trp Ala Ala Val Val Val Pro Ser Gly Glu Glu Gln Arg Tyr Thr 
                245                 250                 255     


Cys His Val Gln His Glu Gly Leu Pro Glu Pro Leu Met Leu Arg Trp 
            260                 265                 270         


Lys Gln Ser Ser Leu Pro Thr Ile Pro Ile Met Gly Ile Val Ala Gly 
        275                 280                 285             


Leu Val Val Leu Ala Ala Val Val Thr Gly Ala Ala Val Ala Ala Val 
    290                 295                 300                 


Leu Trp Arg Lys Lys Ser Ser Asp 
305                 310         


<210>  69
<211>  471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA-G signal peptide-B2M-HLA-G

<400>  69

Met Val Val Met Ala Pro Arg Thr Leu Phe Leu Leu Leu Ser Gly Ala 
1               5                   10                  15      


Leu Thr Leu Thr Glu Thr Trp Ala Arg Ile Ile Pro Arg His Leu Gln 
            20                  25                  30          


Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ile Gln Arg Thr Pro 
        35                  40                  45              


Lys Ile Gln Val Tyr Ser Arg His Pro Ala Glu Asn Gly Lys Ser Asn 
    50                  55                  60                  


Phe Leu Asn Cys Tyr Val Ser Gly Phe His Pro Ser Asp Ile Glu Val 
65                  70                  75                  80  


Asp Leu Leu Lys Asn Gly Glu Arg Ile Glu Lys Val Glu His Ser Asp 
                85                  90                  95      


Leu Ser Phe Ser Lys Asp Trp Ser Phe Tyr Leu Leu Tyr Tyr Thr Glu 
            100                 105                 110         


Phe Thr Pro Thr Glu Lys Asp Glu Tyr Ala Cys Arg Val Asn His Val 
        115                 120                 125             


Thr Leu Ser Gln Pro Lys Ile Val Lys Trp Asp Arg Asp Met Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ser His 
145                 150                 155                 160 


Ser Met Arg Tyr Phe Ser Ala Ala Val Ser Arg Pro Gly Arg Gly Glu 
                165                 170                 175     


Pro Arg Phe Ile Ala Met Gly Tyr Val Asp Asp Thr Gln Phe Val Arg 
            180                 185                 190         


Phe Asp Ser Asp Ser Ala Cys Pro Arg Met Glu Pro Arg Ala Pro Trp 
        195                 200                 205             


Val Glu Gln Glu Gly Pro Glu Tyr Trp Glu Glu Glu Thr Arg Asn Thr 
    210                 215                 220                 


Lys Ala His Ala Gln Thr Asp Arg Met Asn Leu Gln Thr Leu Arg Gly 
225                 230                 235                 240 


Tyr Tyr Asn Gln Ser Glu Ala Ser Ser His Thr Leu Gln Trp Met Ile 
                245                 250                 255     


Gly Cys Asp Leu Gly Ser Asp Gly Arg Leu Leu Arg Gly Tyr Glu Gln 
            260                 265                 270         


Tyr Ala Tyr Asp Gly Lys Asp Tyr Leu Ala Leu Asn Glu Asp Leu Arg 
        275                 280                 285             


Ser Trp Thr Ala Ala Asp Thr Ala Ala Gln Ile Ser Lys Arg Lys Cys 
    290                 295                 300                 


Glu Ala Ala Asn Val Ala Glu Gln Arg Arg Ala Tyr Leu Glu Gly Thr 
305                 310                 315                 320 


Cys Val Glu Trp Leu His Arg Tyr Leu Glu Asn Gly Lys Glu Met Leu 
                325                 330                 335     


Gln Arg Ala Asp Pro Pro Lys Thr His Val Thr His His Pro Val Phe 
            340                 345                 350         


Asp Tyr Glu Ala Thr Leu Arg Cys Trp Ala Leu Gly Phe Tyr Pro Ala 
        355                 360                 365             


Glu Ile Ile Leu Thr Trp Gln Arg Asp Gly Glu Asp Gln Thr Gln Asp 
    370                 375                 380                 


Val Glu Leu Val Glu Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln Lys 
385                 390                 395                 400 


Trp Ala Ala Val Val Val Pro Ser Gly Glu Glu Gln Arg Tyr Thr Cys 
                405                 410                 415     


His Val Gln His Glu Gly Leu Pro Glu Pro Leu Met Leu Arg Trp Lys 
            420                 425                 430         


Gln Ser Ser Leu Pro Thr Ile Pro Ile Met Gly Ile Val Ala Gly Leu 
        435                 440                 445             


Val Val Leu Ala Ala Val Val Thr Gly Ala Ala Val Ala Ala Val Leu 
    450                 455                 460                 


Trp Arg Lys Lys Ser Ser Asp 
465                 470     


<210>  70
<211>  1422
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-G signal peptide-B2M-HLA-G

<400>  70
gccaccatgg tggtcatggc gccccgaacc ctcttcctgc tgctctcggg ggccctgacc       60

ctgaccgaga cctgggcgcg gatcattccc cgacatctgc aactgggagg cggcggttca      120

ggagggggcg gatcgatcca acgcaccccc aagatccagg tctactccag acacccggcc      180

gaaaacggaa agtcgaactt cctgaactgc tatgtgtcag gattccaccc gtccgacatc      240

gaggtggacc tcctgaagaa cggcgaacgc attgagaagg tcgagcactc cgatctgtcg      300

ttctccaagg actggtcctt ctaccttctc tactataccg aattcacccc gaccgagaag      360

gacgaatacg cctgccgggt caaccacgtg accctgagcc agccaaagat cgtgaaatgg      420

gaccgcgata tgggaggagg aggttccggc ggaggaggaa gcggaggcgg aggttccggc      480

tcccactcca tgaggtattt cagcgccgcc gtgtcccggc ctggccgcgg agagcctcgc      540

ttcatcgcca tgggatacgt ggacgacacc cagttcgtca gattcgacag cgacagcgcc      600

tgtcctcgga tggaacctag agcaccttgg gtcgagcaag agggccctga gtactgggaa      660

gaagagacac ggaacaccaa ggctcacgcc cagaccgaca gaatgaacct gcagaccctg      720

cggggctact acaatcagtc tgaggccagc agccatactc tgcagtggat gatcggctgc      780

gatctgggct ctgatggcag actgctgaga ggctacgagc agtacgccta cgacggcaag      840

gattatctgg ccctgaacga ggacctgcgg tcttggacag ctgccgatac agccgctcag      900

atcagcaaga gaaagtgcga ggccgccaat gtggccgaac agagaagggc ttacctggaa      960

ggcacctgtg tggaatggct gcacagatac ctggaaaacg gcaaagagat gctgcagcgg     1020

gccgatcctc ctaagacaca tgtgacccac catcctgtgt tcgactacga ggccacactg     1080

agatgttggg ccctgggctt ttaccctgcc gagatcatcc tgacctggca gcgagatggc     1140

gaggatcaga cccaggatgt ggaactggtg gaaaccagac ctgccggcga cggcaccttt     1200

cagaaatggg ctgctgtggt ggtgcccagc ggagaggaac agagatacac ctgtcacgtg     1260

cagcacgagg gactgcctga acctctgatg ctgagatgga agcagagcag cctgcctaca     1320

atccccatca tgggaatcgt ggccggactg gtggttctgg ccgctgttgt tacaggtgct     1380

gcagtggctg ccgtgctgtg gcggaagaaa agcagcgact ga                        1422


<210>  71
<211>  371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated EGFR (tEGFR)

<400>  71

Met Arg Pro Ser Gly Thr Ala Gly Ala Ala Leu Leu Ala Leu Leu Ala 
1               5                   10                  15      


Ala Leu Cys Pro Ala Ser Arg Ala Gly Val Arg Lys Cys Lys Lys Cys 
            20                  25                  30          


Glu Gly Pro Cys Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe 
        35                  40                  45              


Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn 
    50                  55                  60                  


Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg 
65                  70                  75                  80  


Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp 
                85                  90                  95      


Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala 
            100                 105                 110         


Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile 
        115                 120                 125             


Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val 
    130                 135                 140                 


Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser 
145                 150                 155                 160 


Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn 
                165                 170                 175     


Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys 
            180                 185                 190         


Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val 
        195                 200                 205             


Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg 
    210                 215                 220                 


Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp 
225                 230                 235                 240 


Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser 
                245                 250                 255     


Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile 
            260                 265                 270         


Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr 
        275                 280                 285             


Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly 
    290                 295                 300                 


Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys 
305                 310                 315                 320 


His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu 
                325                 330                 335     


Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly 
            340                 345                 350         


Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly 
        355                 360                 365             


Leu Phe Met 
    370     


<210>  72
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A

<400>  72

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  73
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal Sequence

<400>  73

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Phe Arg Gly 
1               5                   10                  15      


Val Gln Cys 
            


<210>  74
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polivy epitope

<400>  74

Ala Arg Ser Glu Asp Arg Tyr Arg Asn Pro Lys Gly Ser Ala Cys Ser 
1               5                   10                  15      


Arg Ile Trp Gln Ser 
            20      


<210>  75
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD20 mimitope

<400>  75

Ala Cys Pro Tyr Ala Asn Pro Ser Leu Cys 
1               5                   10  


<210>  76
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ErbB epitope

<400>  76

Glu Gly Leu Ala Cys His Gln Leu Cys Ala Arg Gly His Cys Trp Gly 
1               5                   10                  15      


Pro Gly Pro Thr Gln Cys Val Asn Cys Ser Gln Phe Leu Arg Gly Gln 
            20                  25                  30          


Glu Cys Val Glu Glu Cys Arg Val Leu Gln Gly Leu Pro Arg Glu Tyr 
        35                  40                  45              


Val Asn Ala Arg His Cys Leu Pro Cys His Pro Glu Cys Gln Pro Gln 
    50                  55                  60                  


Asn Gly Ser Val Thr Cys Phe Gly Pro Glu Ala Asp Gln Cys Val Ala 
65                  70                  75                  80  


Cys Ala His Tyr Lys Asp Pro Pro Phe Cys Val Ala Arg Cys Pro Ser 
                85                  90                  95      


Gly Val Lys Pro Asp Leu Ser Tyr Met Pro Ile Trp Lys Phe Pro Asp 
            100                 105                 110         


Glu Glu Gly Ala Cys Gln Pro Cys Pro Ile Asn Cys Thr His Ser Cys 
        115                 120                 125             


Val Asp Leu Asp Asp Lys Gly Cys Pro Ala Glu Gln Arg Ala Ser Pro 
    130                 135                 140                 


Leu Thr Ser Ile Ile Ser Ala Val Val Gly Ile Leu Leu Val Val Val 
145                 150                 155                 160 


Leu Gly Val Val Phe Gly Ile Leu Ile Gly Gly Gly Gly Ser Gly Gly 
                165                 170                 175     


<210>  77
<211>  1042
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B2M targeting left homology arm

<400>  77
gcatataaaa cctcagcaga aataaagagg ttttgttgtt tggtaagaac ataccttggg       60

ttggttgggc acggtggctc gtgcctgtaa tcccaacact ttgggaggcc aaggcaggct      120

gatcacttga agttgggagt tcaagaccag cctggccaac atggtgaaat cccgtctcta      180

ctgaaaatac aaaaattaac caggcatggt ggtgtgtgcc tgtagtccca ggaatcactt      240

gaacccagga ggcggaggtt gcagtgagct gagatctcac cactgcacac tgcactccag      300

cctgggcaat ggaatgagat tccatcccaa aaaataaaaa aataaaaaaa taaagaacat      360

accttgggtt gatccactta ggaacctcag ataataacat ctgccacgta tagagcaatt      420

gctatgtccc aggcactcta ctagacactt catacagttt agaaaatcag atgggtgtag      480

atcaaggcag gagcaggaac caaaaagaaa ggcataaaca taagaaaaaa aatggaaggg      540

gtggaaacag agtacaataa catgagtaat ttgatggggg ctattatgaa ctgagaaatg      600

aactttgaaa agtatcttgg ggccaaatca tgtagactct tgagtgatgt gttaaggaat      660

gctatgagtg ctgagagggc atcagaagtc cttgagagcc tccagagaaa ggctcttaaa      720

aatgcagcgc aatctccagt gacagaagat actgctagaa atctgctaga aaaaaaacaa      780

aaaaggcatg tatagaggaa ttatgaggga aagataccaa gtcacggttt attcttcaaa      840

atggaggtgg cttgttggga aggtggaagc tcatttggcc agagtggaaa tggaattggg      900

agaaatcgat gaccaaatgt aaacacttgg tgcctgatat agcttgacac caagttagcc      960

ccaagtgaaa taccctggca atattaatgt gtcttttccc gatattcctc aggtactcca     1020

aagattcagg tttactcacg tc                                              1042


<210>  78
<211>  1023
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B2M targeting right homology arm

<400>  78
atccagcaga gaatggaaag tcaaatttcc tgaattgcta tgtgtctggg tttcatccat       60

ccgacattga agttgactta ctgaagaatg gagagagaat tgaaaaagtg gagcattcag      120

acttgtcttt cagcaaggac tggtctttct atctcttgta ctacactgaa ttcaccccca      180

ctgaaaaaga tgagtatgcc tgccgtgtga accatgtgac tttgtcacag cccaagatag      240

ttaagtgggg taagtcttac attcttttgt aagctgctga aagttgtgta tgagtagtca      300

tatcataaag ctgctttgat ataaaaaagg tctatggcca tactaccctg aatgagtccc      360

atcccatctg atataaacaa tctgcatatt gggattgtca gggaatgttc ttaaagatca      420

gattagtggc acctgctgag atactgatgc acagcatggt ttctgaacca gtagtttccc      480

tgcagttgag cagggagcag cagcagcact tgcacaaata catatacact cttaacactt      540

cttacctact ggcttcctct agcttttgtg gcagcttcag gtatatttag cactgaacga      600

acatctcaag aaggtatagg cctttgtttg taagtcctgc tgtcctagca tcctataatc      660

ctggacttct ccagtacttt ctggctggat tggtatctga ggctagtagg aagggcttgt      720

tcctgctggg tagctctaaa caatgtattc atgggtagga acagcagcct attctgccag      780

ccttatttct aaccatttta gacatttgtt agtacatggt attttaaaag taaaacttaa      840

tgtcttcctt ttttttctcc actgtctttt tcatagatcg agacatgtaa gcagcatcat      900

ggaggtaagt ttttgacctt gagaaaatgt ttttgtttca ctgtcctgag gactatttat      960

agacagctct aacatgataa ccctcactat gtggagaaca ttgacagagt aacattttag     1020

cag                                                                   1023


<210>  79
<211>  7813
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B2M Targeting Plasmid

<400>  79
gcatataaaa cctcagcaga aataaagagg ttttgttgtt tggtaagaac ataccttggg       60

ttggttgggc acggtggctc gtgcctgtaa tcccaacact ttgggaggcc aaggcaggct      120

gatcacttga agttgggagt tcaagaccag cctggccaac atggtgaaat cccgtctcta      180

ctgaaaatac aaaaattaac caggcatggt ggtgtgtgcc tgtagtccca ggaatcactt      240

gaacccagga ggcggaggtt gcagtgagct gagatctcac cactgcacac tgcactccag      300

cctgggcaat ggaatgagat tccatcccaa aaaataaaaa aataaaaaaa taaagaacat      360

accttgggtt gatccactta ggaacctcag ataataacat ctgccacgta tagagcaatt      420

gctatgtccc aggcactcta ctagacactt catacagttt agaaaatcag atgggtgtag      480

atcaaggcag gagcaggaac caaaaagaaa ggcataaaca taagaaaaaa aatggaaggg      540

gtggaaacag agtacaataa catgagtaat ttgatggggg ctattatgaa ctgagaaatg      600

aactttgaaa agtatcttgg ggccaaatca tgtagactct tgagtgatgt gttaaggaat      660

gctatgagtg ctgagagggc atcagaagtc cttgagagcc tccagagaaa ggctcttaaa      720

aatgcagcgc aatctccagt gacagaagat actgctagaa atctgctaga aaaaaaacaa      780

aaaaggcatg tatagaggaa ttatgaggga aagataccaa gtcacggttt attcttcaaa      840

atggaggtgg cttgttggga aggtggaagc tcatttggcc agagtggaaa tggaattggg      900

agaaatcgat gaccaaatgt aaacacttgg tgcctgatat agcttgacac caagttagcc      960

ccaagtgaaa taccctggca atattaatgt gtcttttccc gatattcctc aggtactcca     1020

aagattcagg tttactcacg tcggcctcca acgcgtagat ctattgatta ttgactagtt     1080

attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta     1140

cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt     1200

caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg     1260

tggactattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta     1320

cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga     1380

ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg     1440

gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca     1500

attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg     1560

gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc     1620

ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg     1680

gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgt tgccttcgcc     1740

ccgtgccccg ctccgcgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact     1800

cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta     1860

atgacggctc gtttcttttc tgtggctgcg tgaaagcctt aaagggctcc gggagggccc     1920

tttgtgcggg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg     1980

cgtgcggccc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc     2040

gctccgcgtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cgggggggct     2100

gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg     2160

gcgcggcggt cgggctgtaa cccccccctg cacccccctc cccgagttgc tgagcacggc     2220

ccggcttcgg gtgcggggct ccgtgcgggg cgtggcgcgg ggctcgccgt gccgggcggg     2280

gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg     2340

ggggaggggc gcggcggccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc     2400

attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctggc     2460

ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggc gaagcggtgc     2520

ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc     2580

cttctccatc tccagcctcg gggctgccgc agggggacgg ctgccttcgg gggggacggg     2640

gcagggcggg gttcggcttc tggcgtgtga ccggcgggat atctacgaag cggccgccct     2700

ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta     2760

ttgtgctgtc tcatcatttt ggcaaagtcg acgccaccat ggtggtcatg gcccctagaa     2820

cactgttcct gctgctgtct ggcgccctga cactgacaga gacatgggcc gtgatggccc     2880

ccagaaccct gatcctgggc ggcggtggtt caggcggagg aggttcagga ggagggggta     2940

gtggaggtgg tggttctatc cagcggaccc ctaagatcca ggtgtacagc agacaccccg     3000

ccgagaacgg caagagcaac ttcctgaact gctacgtgtc cggctttcac cccagcgaca     3060

ttgaggtgga cctgctgaag aacggcgagc ggatcgagaa ggtggaacac agcgatctga     3120

gcttcagcaa ggactggtcc ttctacctgc tgtactacac cgagttcacc cctaccgaga     3180

aggacgagta cgcctgcaga gtgaaccacg tgacactgag ccagcctaag atcgtgaagt     3240

gggatcgcga tatgggcgga ggcggatctg gtggcggagg aagtggcggc ggaggatctg     3300

gctcccactc cttgaagtat ttccacactt ccgtgtcccg gcccggccgc ggggagcccc     3360

gcttcatctc tgtgggctac gtggacgaca cccagttcgt gcgcttcgac aacgacgccg     3420

cgagtccgag gatggtgccg cgggcgccgt ggatggagca ggaggggtca gagtattggg     3480

accgggagac acggagcgcc agggacaccg cacagatttt ccgagtgaat ctgcggacgc     3540

tgcgcggcta ctacaatcag agcgaggccg ggtctcacac cctgcagtgg atgcatggct     3600

gcgagctggg gcccgacggg cgcttcctcc gcgggtatga acagttcgcc tacgacggca     3660

aggattatct caccctgaat gaggacctgc gctcctggac cgcggtggac acggcggctc     3720

agatctccga gcaaaagtca aatgatgcct ctgaggcgga gcaccagaga gcctacctgg     3780

aagacacatg cgtggagtgg ctccacaaat acctggagaa ggggaaggag acgctgcttc     3840

acctggagcc cccaaagaca cacgtgactc accaccccat ctctgaccat gaggccaccc     3900

tgaggtgctg ggccctgggc ttctaccctg cggagatcac actgacctgg cagcaggatg     3960

gggagggcca tacccaggac acggagctcg tggagaccag gcctgcaggg gatggaacct     4020

tccagaagtg ggcagctgtg gtggtgcctt ctggagagga gcagagatac acgtgccatg     4080

tgcagcatga ggggctaccc gagcccgtca ccctgagatg gaagccggct tcccagccca     4140

ccatccccat cgtgggcatc attgctggcc tggttctcct tggatctgtg gtctctggag     4200

ctgtggttgc tgctgtgata tggaggaaga agagctcagg tggaaaagga gggagctact     4260

ctaaggctga gtggagcgac agtgcccagg ggtctgagtc tcacagcttg taatgaagcg     4320

gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa     4380

aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa     4440

cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa     4500

taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta     4560

tcatgtctga tccagcagag aatggaaagt caaatttcct gaattgctat gtgtctgggt     4620

ttcatccatc cgacattgaa gttgacttac tgaagaatgg agagagaatt gaaaaagtgg     4680

agcattcaga cttgtctttc agcaaggact ggtctttcta tctcttgtac tacactgaat     4740

tcacccccac tgaaaaagat gagtatgcct gccgtgtgaa ccatgtgact ttgtcacagc     4800

ccaagatagt taagtggggt aagtcttaca ttcttttgta agctgctgaa agttgtgtat     4860

gagtagtcat atcataaagc tgctttgata taaaaaaggt ctatggccat actaccctga     4920

atgagtccca tcccatctga tataaacaat ctgcatattg ggattgtcag ggaatgttct     4980

taaagatcag attagtggca cctgctgaga tactgatgca cagcatggtt tctgaaccag     5040

tagtttccct gcagttgagc agggagcagc agcagcactt gcacaaatac atatacactc     5100

ttaacacttc ttacctactg gcttcctcta gcttttgtgg cagcttcagg tatatttagc     5160

actgaacgaa catctcaaga aggtataggc ctttgtttgt aagtcctgct gtcctagcat     5220

cctataatcc tggacttctc cagtactttc tggctggatt ggtatctgag gctagtagga     5280

agggcttgtt cctgctgggt agctctaaac aatgtattca tgggtaggaa cagcagccta     5340

ttctgccagc cttatttcta accattttag acatttgtta gtacatggta ttttaaaagt     5400

aaaacttaat gtcttccttt tttttctcca ctgtcttttt catagatcga gacatgtaag     5460

cagcatcatg gaggtaagtt tttgaccttg agaaaatgtt tttgtttcac tgtcctgagg     5520

actatttata gacagctcta acatgataac cctcactatg tggagaacat tgacagagta     5580

acattttagc agaggctagg tggaggctca gtgatgataa gtctgcgatg gtggatgcat     5640

gtgtcatggt catagctgtt tcctgtgtga aattgttatc cgctcagagg gcacaatcct     5700

attccgcgct atccgacaat ctccaagaca ttaggtggag ttcagttcgg cgtatggcat     5760

atgtcgctgg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg     5820

ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac     5880

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg     5940

gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct     6000

ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg     6060

tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct     6120

gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac     6180

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt     6240

tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc     6300

tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca     6360

ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat     6420

ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tctattcaac aaagccgccg     6480

tcccgtcaag tcagcgtaaa tgggtagggg gcttcaaatc gtcctcgtga taccaattcg     6540

gagcctgctt ttttgtacaa acttgttgat aatggcaatt caaggatctt cacctagatc     6600

cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct     6660

gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca     6720

tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct     6780

ggccccagtg ctgcaatgat accgcgagag ccacgctcac cggctccaga tttatcagca     6840

ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc     6900

atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg     6960

cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct     7020

tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa     7080

aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta     7140

tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc     7200

ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg     7260

agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa     7320

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg     7380

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc     7440

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg     7500

gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat     7560

cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata     7620

ggggttccgc gcacatttcc ccgaaaagtg ccagatacct gaaacaaaac ccatcgtacg     7680

gccaaggaag tctccaataa ctgtgatcca ccacaagcgc cagggttttc ccagtcacga     7740

cgttgtaaaa cgacggccag tcatgcataa tccgcacgca tctggaataa ggaagtgcca     7800

ttccgcctga cct                                                        7813


<210>  80
<211>  1205
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAVS1 targeting left homology arm

<400>  80
actctgcccc aggcctcctt accattcccc ttcgacctac tctcttccgc attggagtcg       60

ctttaactgg ccctggcttt ggcagcctgt gctgacccat gcagtcctcc ttaccatccc      120

tccctcgact tcccctcttc cgatgttgag cccctccagc cggtcctgga ctttgtctcc      180

ttccctgccc tgccctctcc tgaacctgag ccagctccca tagctcagtc tggtctatct      240

gcctggccct ggccattgtc actttgcgct gccctcctct cgcccccgag tgcccttgct      300

gtgccgccgg aactctgccc tctaacgctg ccgtctctct cctgagtccg gaccactttg      360

agctctactg gcttctgcgc cgcctctggc ccactgtttc cccttcccag gcaggtcctg      420

ctttctctga cctgcattct ctcccctggg cctgtgccgc tttctgtctg cagcttgtgg      480

cctgggtcac ctctacggct ggcccagatc cttccctgcc gcctccttca ggttccgtct      540

tcctccactc cctcttcccc ttgctctctg ctgtgttgct gcccaaggat gctctttccg      600

gagcacttcc ttctcggcgc tgcaccacgt gatgtcctct gagcggatcc tccccgtgtc      660

tgggtcctct ccgggcatct ctcctccctc acccaacccc atgccgtctt cactcgctgg      720

gttccctttt ccttctcctt ctggggcctg tgccatctct cgtttcttag gatggccttc      780

tccgacggat gtctcccttg cgtcccgcct ccccttcttg taggcctgca tcatcaccgt      840

ttttctggac aaccccaaag taccccgtct ccctggcttt agccacctct ccatcctctt      900

gctttctttg cctggacacc ccgttctcct gtggattcgg gtcacctctc actcctttca      960

tttgggcagc tcccctaccc cccttacctc tctagtctgt gctagctctt ccagccccct     1020

gtcatggcat cttccagggg tccgagagct cagctagtct tcttcctcca acccgggccc     1080

ctatgtccac ttcaggacag catgtttgct gcctccaggg atcctgtgtc cccgagctgg     1140

gaccacctta tattcccagg gccggttaat gtggctctgg ttctgggtac ttttatctgt     1200

cccct                                                                 1205


<210>  81
<211>  1200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAVS1 targeting right homology arm

<400>  81
ccaccccaca gtggggccac tagggacagg attggtgaca gaaaagcccc atccttaggc       60

ctcctccttc ctagtctcct gatattgggt ctaaccccca cctcctgtta ggcagattcc      120

ttatctggtg acacaccccc atttcctgga gccatctctc tccttgccag aacctctaag      180

gtttgcttac gatggagcca gagaggatcc tgggagggag agcttggcag ggggtgggag      240

ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc accctgcgct accctctccc      300

agaacctgag ctgctctgac gcggccgtct ggtgcgtttc actgatcctg gtgctgcagc      360

ttccttacac ttcccaagag gagaagcagt ttggaaaaac aaaatcagaa taagttggtc      420

ctgagttcta actttggctc ttcacctttc tagtccccaa tttatattgt tcctccgtgc      480

gtcagtttta cctgtgagat aaggccagta gccagccccg tcctggcagg gctgtggtga      540

ggaggggggt gtccgtgtgg aaaactccct ttgtgagaat ggtgcgtcct aggtgttcac      600

caggtcgtgg ccgcctctac tccctttctc tttctccatc cttctttcct taaagagtcc      660

ccagtgctat ctgggacata ttcctccgcc cagagcaggg tcccgcttcc ctaaggccct      720

gctctgggct tctgggtttg agtccttggc aagcccagga gaggcgctca ggcttccctg      780

tcccccttcc tcgtccacca tctcatgccc ctggctctcc tgccccttcc ctacaggggt      840

tcctggctct gctcttcaga ctgagccccg ttcccctgca tccccgtacc cctgcatccc      900

ccttcccctg catcccccag aggccccagg ccacctactt ggcctggacc ccacgagagg      960

ccaccccagc cctgtctacc aggctgcctt ttgggtggat tctcctccaa ctgtggggtg     1020

actgcttggc aaactcactc ttcggggtat cccaggaggc ctggagcatt ggggtgggct     1080

ggggttcaga gaggagggat tcccttctca ggttacgtgg ccaagaagca ggggagctgg     1140

gtttgggtca ggtctgggtg tggggtgacc agcttatgct gtttgcccag gacagcctag     1200


<210>  82
<211>  7971
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAVS1 Targeting plasmid

<400>  82
actctgcccc aggcctcctt accattcccc ttcgacctac tctcttccgc attggagtcg       60

ctttaactgg ccctggcttt ggcagcctgt gctgacccat gcagtcctcc ttaccatccc      120

tccctcgact tcccctcttc cgatgttgag cccctccagc cggtcctgga ctttgtctcc      180

ttccctgccc tgccctctcc tgaacctgag ccagctccca tagctcagtc tggtctatct      240

gcctggccct ggccattgtc actttgcgct gccctcctct cgcccccgag tgcccttgct      300

gtgccgccgg aactctgccc tctaacgctg ccgtctctct cctgagtccg gaccactttg      360

agctctactg gcttctgcgc cgcctctggc ccactgtttc cccttcccag gcaggtcctg      420

ctttctctga cctgcattct ctcccctggg cctgtgccgc tttctgtctg cagcttgtgg      480

cctgggtcac ctctacggct ggcccagatc cttccctgcc gcctccttca ggttccgtct      540

tcctccactc cctcttcccc ttgctctctg ctgtgttgct gcccaaggat gctctttccg      600

gagcacttcc ttctcggcgc tgcaccacgt gatgtcctct gagcggatcc tccccgtgtc      660

tgggtcctct ccgggcatct ctcctccctc acccaacccc atgccgtctt cactcgctgg      720

gttccctttt ccttctcctt ctggggcctg tgccatctct cgtttcttag gatggccttc      780

tccgacggat gtctcccttg cgtcccgcct ccccttcttg taggcctgca tcatcaccgt      840

ttttctggac aaccccaaag taccccgtct ccctggcttt agccacctct ccatcctctt      900

gctttctttg cctggacacc ccgttctcct gtggattcgg gtcacctctc actcctttca      960

tttgggcagc tcccctaccc cccttacctc tctagtctgt gctagctctt ccagccccct     1020

gtcatggcat cttccagggg tccgagagct cagctagtct tcttcctcca acccgggccc     1080

ctatgtccac ttcaggacag catgtttgct gcctccaggg atcctgtgtc cccgagctgg     1140

gaccacctta tattcccagg gccggttaat gtggctctgg ttctgggtac ttttatctgt     1200

cccctgcggc cgcacgcgta gatctattga ttattgacta gttattaata gtaatcaatt     1260

acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat     1320

ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt     1380

cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggacta tttacggtaa     1440

actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc     1500

aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct     1560

acttggcagt acatctacgt attagtcatc gctattacca tgggtcgagg tgagccccac     1620

gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat     1680

tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg     1740

cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga     1800

gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa     1860

agcgaagcgc gcggcgggcg ggagtcgctg cgttgccttc gccccgtgcc ccgctccgcg     1920

ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc     1980

gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg ctcgtttctt     2040

ttctgtggct gcgtgaaagc cttaaagggc tccgggaggg ccctttgtgc gggggggagc     2100

ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg cccgcgctgc     2160

ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc gtgtgcgcga     2220

ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg gctgcgaggg gaacaaaggc     2280

tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcggc ggtcgggctg     2340

taaccccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg     2400

gctccgtgcg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg     2460

ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg     2520

ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa     2580

tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct ggcggagccg aaatctggga     2640

ggcgccgccg caccccctct agcgggcgcg ggcgaagcgg tgcggcgccg gcaggaagga     2700

aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc atctccagcc     2760

tcggggctgc cgcaggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc     2820

ttctggcgtg tgaccggcgg gatatctacg aagcggccgc cctctgctaa ccatgttcat     2880

gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat     2940

tttggcaaag tcgacgccac catgctgctg ctggtcacat ctctgctgct gtgcgagctg     3000

ccccatcctg cctttctgct gatccccgac atccagatga cccagaccac aagcagcctg     3060

tctgccagcc tgggcgatag agtgaccatc agctgtagag ccagccagga catcagcaag     3120

tacctgaact ggtatcagca aaagcccgac ggcaccgtga agctgctgat ctaccacacc     3180

agcagactgc acagcggcgt gccaagcaga ttttctggca gcggctctgg caccgactac     3240

agcctgacaa tcagcaacct ggaacaagag gatatcgcta cctacttctg ccagcaaggc     3300

aacaccctgc cttacacctt tggcggaggc accaagctgg aaatcaccgg ctctacaagc     3360

ggcagcggca aacctggatc tggcgaggga tctaccaagg gcgaagtgaa actgcaagag     3420

tctggccctg gactggtggc cccatctcag tctctgagcg tgacctgtac agtcagcgga     3480

gtgtccctgc ctgattacgg cgtgtcctgg atcagacagc ctcctcggaa aggcctggaa     3540

tggctgggag tgatctgggg cagcgagaca acctactaca acagcgccct gaagtcccgg     3600

ctgaccatca tcaaggacaa ctccaagagc caggtgttcc tgaagatgaa cagcctgcag     3660

accgacgaca ccgccatcta ctattgcgcc aagcactact actacggcgg cagctacgcc     3720

atggattatt ggggccaggg caccagcgtg accgtgtcta gcacgacgac tcctgctcca     3780

aggcctccta cacctgcacc aaccattgca agtcagccgt tgagcctccg gccagaagca     3840

tgtcgcccag ccgcaggcgg ggctgtacac acgagaggct tggatttcgc atgtgacatc     3900

tatatctggg ccccactggc cggcacctgc ggcgtgctgc tgctgagcct ggtgatcacc     3960

aagcgaggcc gcaaaaaact cctttatata ttcaagcaac cttttatgag gcccgtccag     4020

accacgcaag aggaagatgg gtgctcttgc cgctttccag aggaagagga ggggggctgc     4080

gaacttagag tgaagttcag cagatccgcc gatgctcccg cctatcagca gggccaaaac     4140

cagctgtaca acgagctgaa cctggggaga agagaagagt acgacgtgct ggacaagcgg     4200

agaggcagag atcctgaaat gggcggcaag cccagacgga agaatcctca agagggcctg     4260

tataatgagc tgcagaaaga caagatggcc gaggcctaca gcgagatcgg aatgaagggc     4320

gagcgcagaa gaggcaaggg acacgatgga ctgtaccagg gcctgagcac cgccaccaag     4380

gatacctatg atgccctgca catgcaggcc ctgcctccaa gataataaaa cttgtttatt     4440

gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt     4500

ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta ccaccccaca     4560

gtggggccac tagggacagg attggtgaca gaaaagcccc atccttaggc ctcctccttc     4620

ctagtctcct gatattgggt ctaaccccca cctcctgtta ggcagattcc ttatctggtg     4680

acacaccccc atttcctgga gccatctctc tccttgccag aacctctaag gtttgcttac     4740

gatggagcca gagaggatcc tgggagggag agcttggcag ggggtgggag ggaagggggg     4800

gatgcgtgac ctgcccggtt ctcagtggcc accctgcgct accctctccc agaacctgag     4860

ctgctctgac gcggccgtct ggtgcgtttc actgatcctg gtgctgcagc ttccttacac     4920

ttcccaagag gagaagcagt ttggaaaaac aaaatcagaa taagttggtc ctgagttcta     4980

actttggctc ttcacctttc tagtccccaa tttatattgt tcctccgtgc gtcagtttta     5040

cctgtgagat aaggccagta gccagccccg tcctggcagg gctgtggtga ggaggggggt     5100

gtccgtgtgg aaaactccct ttgtgagaat ggtgcgtcct aggtgttcac caggtcgtgg     5160

ccgcctctac tccctttctc tttctccatc cttctttcct taaagagtcc ccagtgctat     5220

ctgggacata ttcctccgcc cagagcaggg tcccgcttcc ctaaggccct gctctgggct     5280

tctgggtttg agtccttggc aagcccagga gaggcgctca ggcttccctg tcccccttcc     5340

tcgtccacca tctcatgccc ctggctctcc tgccccttcc ctacaggggt tcctggctct     5400

gctcttcaga ctgagccccg ttcccctgca tccccgtacc cctgcatccc ccttcccctg     5460

catcccccag aggccccagg ccacctactt ggcctggacc ccacgagagg ccaccccagc     5520

cctgtctacc aggctgcctt ttgggtggat tctcctccaa ctgtggggtg actgcttggc     5580

aaactcactc ttcggggtat cccaggaggc ctggagcatt ggggtgggct ggggttcaga     5640

gaggagggat tcccttctca ggttacgtgg ccaagaagca ggggagctgg gtttgggtca     5700

ggtctgggtg tggggtgacc agcttatgct gtttgcccag gacagcctag aggctaggtg     5760

gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca tagctgtttc     5820

ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat ccgacaatct     5880

ccaagacatt aggtggagtt cagttcggcg tatggcatat gtcgctggaa agaacatgtg     5940

agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca     6000

taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa     6060

cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc     6120

tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc     6180

gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct     6240

gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg     6300

tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag     6360

gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta     6420

cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg     6480

aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt     6540

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt     6600

ttctacgggg tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc agcgtaaatg     6660

ggtagggggc ttcaaatcgt cctcgtgata ccaattcgga gcctgctttt ttgtacaaac     6720

ttgttgataa tggcaattca aggatcttca cctagatcct tttaaattaa aaatgaagtt     6780

ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca     6840

gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg     6900

tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac     6960

cgcgagagcc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg     7020

ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc     7080

gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta     7140

caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac     7200

gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc     7260

ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac     7320

tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact     7380

caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa     7440

tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt     7500

cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca     7560

ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa     7620

aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac     7680

tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg     7740

gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc     7800

gaaaagtgcc agatacctga aacaaaaccc atcgtacggc caaggaagtc tccaataact     7860

gtgatccacc acaagcgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtc     7920

atgcataatc cgcacgcatc tggaataagg aagtgccatt ccgcctgacc t              7971


<210>  83
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Influenza Peptide

<400>  83

Gly Ile Leu Gly Phe Val Phe Thr Leu 
1               5                   


<210>  84
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TCR alpaha CDR3

<400>  84

Cys Ala Gly Ala Gly Ser Gln Gly Asn Leu Ile Phe 
1               5                   10          


<210>  85
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TCR beta CDR3

<400>  85

Cys Ala Ser Ser Ile Arg Ser Ser Tyr Glu Gln Tyr Phe 
1               5                   10              


<210>  86
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TCR alpha chain

<400>  86

Met Val Leu Lys Phe Ser Val Ser Ile Leu Trp Ile Gln Leu Ala Trp 
1               5                   10                  15      


Val Ser Thr Gln Leu Leu Glu Gln Ser Pro Gln Phe Leu Ser Ile Gln 
            20                  25                  30          


Glu Gly Glu Asn Leu Thr Val Tyr Cys Asn Ser Ser Ser Val Phe Ser 
        35                  40                  45              


Ser Leu Gln Trp Tyr Arg Gln Glu Pro Gly Glu Gly Pro Val Leu Leu 
    50                  55                  60                  


Val Thr Val Val Thr Gly Gly Glu Val Lys Lys Leu Lys Arg Leu Thr 
65                  70                  75                  80  


Phe Gln Phe Gly Asp Ala Arg Lys Asp Ser Ser Leu His Ile Thr Ala 
                85                  90                  95      


Ala Gln Pro Gly Asp Thr Gly Leu Tyr Leu Cys Ala Gly Ala Gly Ser 
            100                 105                 110         


Gln Gly Asn Leu Ile Phe Gly Lys Gly Thr Lys Leu Ser Val Lys Pro 
        115                 120                 125             


Asn Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys 
    130                 135                 140                 


Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr 
145                 150                 155                 160 


Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr 
                165                 170                 175     


Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala 
            180                 185                 190         


Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser 
        195                 200                 205             


Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp 
    210                 215                 220                 


Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe 
225                 230                 235                 240 


Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala 
                245                 250                 255     


Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
            260                 265                 


<210>  87
<211>  310
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TCR beta chain

<400>  87

Met Ser Asn Gln Val Leu Cys Cys Val Val Leu Cys Phe Leu Gly Ala 
1               5                   10                  15      


Asn Thr Val Asp Gly Gly Ile Thr Gln Ser Pro Lys Tyr Leu Phe Arg 
            20                  25                  30          


Lys Glu Gly Gln Asn Val Thr Leu Ser Cys Glu Gln Asn Leu Asn His 
        35                  40                  45              


Asp Ala Met Tyr Trp Tyr Arg Gln Asp Pro Gly Gln Gly Leu Arg Leu 
    50                  55                  60                  


Ile Tyr Tyr Ser Gln Ile Val Asn Asp Phe Gln Lys Gly Asp Ile Ala 
65                  70                  75                  80  


Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu Ser Phe Pro Leu Thr 
                85                  90                  95      


Val Thr Ser Ala Gln Lys Asn Pro Thr Ala Phe Tyr Leu Cys Ala Ser 
            100                 105                 110         


Ser Ile Arg Ser Ser Tyr Glu Gln Tyr Phe Gly Pro Gly Thr Arg Leu 
        115                 120                 125             


Thr Val Thr Glu Asp Leu Lys Asn Val Phe Pro Pro Lys Val Ala Val 
    130                 135                 140                 


Phe Glu Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu 
145                 150                 155                 160 


Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp 
                165                 170                 175     


Trp Val Asn Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln 
            180                 185                 190         


Pro Leu Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser 
        195                 200                 205             


Ser Arg Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His 
    210                 215                 220                 


Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp 
225                 230                 235                 240 


Thr Gln Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala 
                245                 250                 255     


Trp Gly Arg Ala Asp Cys Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly 
            260                 265                 270         


Val Leu Ser Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr 
        275                 280                 285             


Leu Tyr Ala Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys 
    290                 295                 300                 


Arg Lys Asp Ser Arg Gly 
305                 310 


<210>  88
<211>  810
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TCR alpha chain

<400>  88
atggtactca aattctcagt gtcaattctg tggatccaac tggcttgggt gagcacacaa       60

ctgctggagc agtctcctca atttcttagc atacaagaag gagaaaattt gacagtttac      120

tgtaacagca gttccgtttt ttcctcactg caatggtatc gacaagaacc aggtgagggt      180

ccggtccttc tcgtcacagt cgtcaccggg ggggaagtca agaagctgaa gagactgacc      240

tttcaattcg gtgacgcacg gaaggattcc agccttcaca taaccgccgc ccaaccaggt      300

gacacaggcc tgtacctgtg tgccggggca gggtcacaag gcaacctgat tttcggcaaa      360

ggcacaaaac tttccgtaaa accaaacatc caaaaccctg acccggctgt ttatcagctg      420

cgcgactcca agtcatctga taaatcagtg tgtctgttca cggattttga tagccaaact      480

aacgtgagcc aatcaaagga ctcagacgta tacattacgg ataaaactgt attggacatg      540

cgatcaatgg acttcaaaag caactccgcg gttgcatggt caaataaatc cgatttcgca      600

tgcgccaatg catttaacaa ctctataatc ccggaagata cgttttttcc cagtcccgaa      660

agtagttgcg acgtgaaact cgtggagaag tctttcgaga cagatactaa tctgaacttc      720

cagaacctca gcgtgatagg gttccgcata ctcctgttga aagtagctgg gttcaatctt      780

ttgatgacat tgcgcctttg gtcttcttga                                       810


<210>  89
<211>  933
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TCR beta chain

<400>  89
atgtccaatc aggtgttgtg ctgcgtagta ttgtgttttt tgggggcaaa caccgttgac       60

ggaggtatta ctcagtcacc caagtacctg ttcagaaaag aaggtcagaa tgttacattg      120

tcctgtgagc agaacctgaa tcacgacgct atgtattggt atcgccaaga tcccggccaa      180

ggacttagat tgatttatta tagtcaaatc gttaacgatt tccaaaaagg agacattgcg      240

gaaggttata gtgtatcaag ggagaagaaa gaatcattcc cgctgacagt tactagtgct      300

cagaaaaacc ccactgcctt ctatttgtgt gcttcttcta tccgctctag ttacgaacaa      360

tactttggtc cgggtacccg acttacggtc accgaagacc tgaaaaacgt ctttcccccg      420

aaagttgcag tgtttgagcc cagcgaggca gaaatcagtc acacccaaaa agctacattg      480

gtgtgccttg ctactgggtt ctacccagac cacgtggagc ttagctggtg ggttaacggg      540

aaagaagtac acagtggtgt atccaccgat ccccagcctt tgaaagaaca acccgctctt      600

aacgattctc gctactgcct cagtagtaga ttgagggtat cagcgacatt ctggcagaat      660

ccgcgcaacc atttcagatg tcaggtccaa ttctatggct tgtcagagaa cgacgaatgg      720

actcaggacc gagctaaacc cgttacgcag atcgtgtcag cagaggcttg ggggagggct      780

gactgtgggt tcacctcaga gtcttaccaa cagggcgttc ttagcgcgac cattttgtac      840

gaaatcttgc tgggaaaagc cactctctac gccgttcttg tcagcgcgct cgtgcttatg      900

gcgatggtta agagaaagga ttcacgggga tga                                   933


<210>  90
<211>  601
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DLL4-Fc Fusion 1

<400>  90

Ser Gly Val Phe Gln Leu Gln Leu Gln Glu Phe Ile Asn Glu Arg Gly 
1               5                   10                  15      


Val Leu Ala Ser Gly Arg Pro Cys Glu Pro Gly Cys Arg Thr Phe Phe 
            20                  25                  30          


Arg Val Cys Leu Lys His Phe Gln Ala Val Val Ser Pro Gly Pro Cys 
        35                  40                  45              


Thr Phe Gly Thr Val Ser Thr Pro Val Leu Gly Thr Asn Ser Phe Ala 
    50                  55                  60                  


Val Arg Asp Asp Ser Ser Gly Gly Gly Arg Asn Pro Leu Gln Leu Pro 
65                  70                  75                  80  


Phe Asn Phe Thr Trp Pro Gly Thr Phe Ser Leu Ile Ile Glu Ala Trp 
                85                  90                  95      


His Ala Pro Gly Asp Asp Leu Arg Pro Glu Ala Leu Pro Pro Asp Ala 
            100                 105                 110         


Leu Ile Ser Lys Ile Ala Ile Gln Gly Ser Leu Ala Val Gly Gln Asn 
        115                 120                 125             


Trp Leu Leu Asp Glu Gln Thr Ser Thr Leu Thr Arg Leu Arg Tyr Ser 
    130                 135                 140                 


Tyr Arg Val Ile Cys Ser Asp Asn Tyr Tyr Gly Asp Asn Cys Ser Arg 
145                 150                 155                 160 


Leu Cys Lys Lys Arg Asn Asp His Phe Gly His Tyr Val Cys Gln Pro 
                165                 170                 175     


Asp Gly Asn Leu Ser Cys Leu Pro Gly Trp Thr Gly Glu Tyr Cys Gln 
            180                 185                 190         


Gln Pro Ile Cys Leu Ser Gly Cys His Glu Gln Asn Gly Tyr Cys Ser 
        195                 200                 205             


Lys Pro Ala Glu Cys Leu Cys Arg Pro Gly Trp Gln Gly Arg Leu Cys 
    210                 215                 220                 


Asn Glu Cys Ile Pro His Asn Gly Cys Arg His Gly Thr Cys Ser Thr 
225                 230                 235                 240 


Pro Trp Gln Cys Thr Cys Asp Glu Gly Trp Gly Gly Leu Phe Cys Asp 
                245                 250                 255     


Gln Asp Leu Asn Tyr Cys Thr His His Ser Pro Cys Lys Asn Gly Ala 
            260                 265                 270         


Thr Cys Ser Asn Ser Gly Gln Arg Ser Tyr Thr Cys Thr Cys Arg Pro 
        275                 280                 285             


Gly Tyr Thr Gly Val Asp Cys Glu Leu Glu Leu Ser Glu Cys Asp Ser 
    290                 295                 300                 


Asn Pro Cys Arg Asn Gly Gly Ser Cys Lys Asp Gln Glu Asp Gly Tyr 
305                 310                 315                 320 


His Cys Leu Cys Pro Pro Gly Tyr Tyr Gly Leu His Cys Glu His Ser 
                325                 330                 335     


Thr Leu Ser Cys Ala Asp Ser Pro Cys Phe Asn Gly Gly Ser Cys Arg 
            340                 345                 350         


Glu Arg Asn Gln Gly Ala Asn Tyr Ala Cys Glu Cys Pro Pro Asn Phe 
        355                 360                 365             


Thr Gly Ser Asn Cys Glu Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
    370                 375                 380                 


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
385                 390                 395                 400 


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
                405                 410                 415     


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
            420                 425                 430         


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
        435                 440                 445             


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
    450                 455                 460                 


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
465                 470                 475                 480 


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
                485                 490                 495     


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
            500                 505                 510         


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
        515                 520                 525             


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
    530                 535                 540                 


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
545                 550                 555                 560 


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
                565                 570                 575     


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
            580                 585                 590         


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        595                 600     


<210>  91
<211>  484
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DLL4-Fc Fusion 2

<400>  91

Ser Gly Val Phe Gln Leu Gln Leu Gln Glu Phe Ile Asn Glu Arg Gly 
1               5                   10                  15      


Val Leu Ala Ser Gly Arg Pro Cys Glu Pro Gly Cys Arg Thr Phe Phe 
            20                  25                  30          


Arg Val Cys Leu Lys His Phe Gln Ala Val Val Ser Pro Gly Pro Cys 
        35                  40                  45              


Thr Phe Gly Thr Val Ser Thr Pro Val Leu Gly Thr Asn Ser Phe Ala 
    50                  55                  60                  


Val Arg Asp Asp Ser Ser Gly Gly Gly Arg Asn Pro Leu Gln Leu Pro 
65                  70                  75                  80  


Phe Asn Phe Thr Trp Pro Gly Thr Phe Ser Leu Ile Ile Glu Ala Trp 
                85                  90                  95      


His Ala Pro Gly Asp Asp Leu Arg Pro Glu Ala Leu Pro Pro Asp Ala 
            100                 105                 110         


Leu Ile Ser Lys Ile Ala Ile Gln Gly Ser Leu Ala Val Gly Gln Asn 
        115                 120                 125             


Trp Leu Leu Asp Glu Gln Thr Ser Thr Leu Thr Arg Leu Arg Tyr Ser 
    130                 135                 140                 


Tyr Arg Val Ile Cys Ser Asp Asn Tyr Tyr Gly Asp Asn Cys Ser Arg 
145                 150                 155                 160 


Leu Cys Lys Lys Arg Asn Asp His Phe Gly His Tyr Val Cys Gln Pro 
                165                 170                 175     


Asp Gly Asn Leu Ser Cys Leu Pro Gly Trp Thr Gly Glu Tyr Cys Gln 
            180                 185                 190         


Gln Pro Ile Cys Leu Ser Gly Cys His Glu Gln Asn Gly Tyr Cys Ser 
        195                 200                 205             


Lys Pro Ala Glu Cys Leu Cys Arg Pro Gly Trp Gln Gly Arg Leu Cys 
    210                 215                 220                 


Asn Glu Cys Ile Pro His Asn Gly Cys Arg His Gly Thr Cys Ser Thr 
225                 230                 235                 240 


Pro Trp Gln Cys Thr Cys Asp Glu Gly Trp Gly Gly Leu Phe Cys Asp 
                245                 250                 255     


Gln Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
            260                 265                 270         


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
        275                 280                 285             


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
    290                 295                 300                 


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
305                 310                 315                 320 


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
                325                 330                 335     


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
            340                 345                 350         


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
        355                 360                 365             


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
    370                 375                 380                 


Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
385                 390                 395                 400 


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
                405                 410                 415     


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
            420                 425                 430         


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
        435                 440                 445             


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
    450                 455                 460                 


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
465                 470                 475                 480 


Ser Pro Gly Lys 
                


<210>  92
<211>  725
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DLL4-Fc Fusion 3

<400>  92

Ser Gly Val Phe Gln Leu Gln Leu Gln Glu Phe Ile Asn Glu Arg Gly 
1               5                   10                  15      


Val Leu Ala Ser Gly Arg Pro Cys Glu Pro Gly Cys Arg Thr Phe Phe 
            20                  25                  30          


Arg Val Cys Leu Lys His Phe Gln Ala Val Val Ser Pro Gly Pro Cys 
        35                  40                  45              


Thr Phe Gly Thr Val Ser Thr Pro Val Leu Gly Thr Asn Ser Phe Ala 
    50                  55                  60                  


Val Arg Asp Asp Ser Ser Gly Gly Gly Arg Asn Pro Leu Gln Leu Pro 
65                  70                  75                  80  


Phe Asn Phe Thr Trp Pro Gly Thr Phe Ser Leu Ile Ile Glu Ala Trp 
                85                  90                  95      


His Ala Pro Gly Asp Asp Leu Arg Pro Glu Ala Leu Pro Pro Asp Ala 
            100                 105                 110         


Leu Ile Ser Lys Ile Ala Ile Gln Gly Ser Leu Ala Val Gly Gln Asn 
        115                 120                 125             


Trp Leu Leu Asp Glu Gln Thr Ser Thr Leu Thr Arg Leu Arg Tyr Ser 
    130                 135                 140                 


Tyr Arg Val Ile Cys Ser Asp Asn Tyr Tyr Gly Asp Asn Cys Ser Arg 
145                 150                 155                 160 


Leu Cys Lys Lys Arg Asn Asp His Phe Gly His Tyr Val Cys Gln Pro 
                165                 170                 175     


Asp Gly Asn Leu Ser Cys Leu Pro Gly Trp Thr Gly Glu Tyr Cys Gln 
            180                 185                 190         


Gln Pro Ile Cys Leu Ser Gly Cys His Glu Gln Asn Gly Tyr Cys Ser 
        195                 200                 205             


Lys Pro Ala Glu Cys Leu Cys Arg Pro Gly Trp Gln Gly Arg Leu Cys 
    210                 215                 220                 


Asn Glu Cys Ile Pro His Asn Gly Cys Arg His Gly Thr Cys Ser Thr 
225                 230                 235                 240 


Pro Trp Gln Cys Thr Cys Asp Glu Gly Trp Gly Gly Leu Phe Cys Asp 
                245                 250                 255     


Gln Asp Leu Asn Tyr Cys Thr His His Ser Pro Cys Lys Asn Gly Ala 
            260                 265                 270         


Thr Cys Ser Asn Ser Gly Gln Arg Ser Tyr Thr Cys Thr Cys Arg Pro 
        275                 280                 285             


Gly Tyr Thr Gly Val Asp Cys Glu Leu Glu Leu Ser Glu Cys Asp Ser 
    290                 295                 300                 


Asn Pro Cys Arg Asn Gly Gly Ser Cys Lys Asp Gln Glu Asp Gly Tyr 
305                 310                 315                 320 


His Cys Leu Cys Pro Pro Gly Tyr Tyr Gly Leu His Cys Glu His Ser 
                325                 330                 335     


Thr Leu Ser Cys Ala Asp Ser Pro Cys Phe Asn Gly Gly Ser Cys Arg 
            340                 345                 350         


Glu Arg Asn Gln Gly Ala Asn Tyr Ala Cys Glu Cys Pro Pro Asn Phe 
        355                 360                 365             


Thr Gly Ser Asn Cys Glu Lys Lys Val Asp Arg Cys Thr Ser Asn Pro 
    370                 375                 380                 


Cys Ala Asn Gly Gly Gln Cys Leu Asn Arg Gly Pro Ser Arg Met Cys 
385                 390                 395                 400 


Arg Cys Arg Pro Gly Phe Thr Gly Thr Tyr Cys Glu Leu His Val Ser 
                405                 410                 415     


Asp Cys Ala Arg Asn Pro Cys Ala His Gly Gly Thr Cys His Asp Leu 
            420                 425                 430         


Glu Asn Gly Leu Met Cys Thr Cys Pro Ala Gly Phe Ser Gly Arg Arg 
        435                 440                 445             


Cys Glu Val Arg Thr Ser Ile Asp Ala Cys Ala Ser Ser Pro Cys Phe 
    450                 455                 460                 


Asn Arg Ala Thr Cys Tyr Thr Asp Leu Ser Thr Asp Thr Phe Val Cys 
465                 470                 475                 480 


Asn Cys Pro Tyr Gly Phe Val Gly Ser Arg Cys Glu Phe Pro Val Gly 
                485                 490                 495     


Leu Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
            500                 505                 510         


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
        515                 520                 525             


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
    530                 535                 540                 


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
545                 550                 555                 560 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
                565                 570                 575     


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
            580                 585                 590         


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
        595                 600                 605             


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
    610                 615                 620                 


Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln 
625                 630                 635                 640 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
                645                 650                 655     


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
            660                 665                 670         


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
        675                 680                 685             


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
    690                 695                 700                 


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
705                 710                 715                 720 


Leu Ser Pro Gly Lys 
                725 


<210>  93
<211>  484
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DLL4-Fc Fusion 4

<400>  93

Ser Ser Val Phe Gln Leu Gln Leu Gln Glu Phe Ile Asn Glu Arg Gly 
1               5                   10                  15      


Val Leu Ala Ser Gly Arg Pro Cys Glu Pro Gly Cys Arg Thr Phe Phe 
            20                  25                  30          


Arg Val Cys Leu Lys His Phe Gln Ala Val Val Ser Pro Gly Pro Cys 
        35                  40                  45              


Thr Phe Gly Thr Val Ser Thr Pro Val Leu Gly Thr Asn Ser Phe Ala 
    50                  55                  60                  


Val Arg Asp Asp Ser Ser Gly Gly Gly Arg Asn Pro Leu Gln Leu Pro 
65                  70                  75                  80  


Leu Asn Phe Thr Trp Pro Gly Thr Phe Ser Leu Ile Ile Glu Ala Trp 
                85                  90                  95      


His Ala Pro Gly Asp Asp Leu Arg Pro Glu Ala Leu Pro Pro Asp Ala 
            100                 105                 110         


Leu Ile Ser Lys Phe Ala Ile Gln Gly Ser Leu Ala Val Gly Gln Asn 
        115                 120                 125             


Trp Leu Leu Asp Glu Gln Thr Ser Thr Leu Thr Arg Leu Arg Tyr Ser 
    130                 135                 140                 


Tyr Arg Val Ile Cys Ser Asp Asn Tyr Tyr Gly Asp Asn Cys Ser Arg 
145                 150                 155                 160 


Leu Cys Lys Lys Arg Asn Asp Tyr Phe Gly His Tyr Val Cys Gln Pro 
                165                 170                 175     


Asp Gly Asn Pro Ser Cys Leu Pro Gly Trp Thr Gly Glu Tyr Cys Gln 
            180                 185                 190         


Gln Pro Ile Cys Leu Ser Gly Cys His Glu Gln Asn Gly Tyr Cys Ser 
        195                 200                 205             


Lys Pro Ala Glu Cys Leu Cys Arg Pro Gly Trp Gln Gly Arg Leu Cys 
    210                 215                 220                 


Asn Glu Cys Ile Pro His Asn Gly Cys Arg His Gly Thr Cys Ser Thr 
225                 230                 235                 240 


Pro Trp Gln Cys Thr Cys Asp Glu Gly Trp Gly Gly Leu Phe Cys Asp 
                245                 250                 255     


Gln Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
            260                 265                 270         


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
        275                 280                 285             


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
    290                 295                 300                 


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
305                 310                 315                 320 


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
                325                 330                 335     


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
            340                 345                 350         


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
        355                 360                 365             


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
    370                 375                 380                 


Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
385                 390                 395                 400 


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
                405                 410                 415     


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
            420                 425                 430         


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
        435                 440                 445             


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
    450                 455                 460                 


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
465                 470                 475                 480 


Ser Pro Gly Lys 
                


<210>  94
<211>  484
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DLL4-Fc Fusion 5

<400>  94

Ser Ser Val Phe Gln Leu Gln Leu Gln Glu Phe Ile Asn Glu Arg Gly 
1               5                   10                  15      


Val Leu Ala Ser Gly Arg Pro Cys Glu Pro Gly Cys Arg Thr Phe Phe 
            20                  25                  30          


Arg Val Cys Leu Lys His Phe Gln Ala Val Val Ser Pro Gly Pro Cys 
        35                  40                  45              


Thr Phe Gly Thr Val Ser Thr Pro Val Leu Gly Thr Asn Ser Phe Ala 
    50                  55                  60                  


Val Arg Asp Asp Ser Ser Gly Gly Gly Arg Asn Pro Leu Gln Leu Pro 
65                  70                  75                  80  


Leu Asn Phe Thr Trp Pro Gly Thr Phe Ser Leu Ile Ile Glu Ala Trp 
                85                  90                  95      


His Ala Pro Gly Asp Asp Leu Arg Pro Glu Ala Leu Pro Pro Asp Ala 
            100                 105                 110         


Leu Ile Ser Lys Phe Ala Ile Gln Gly Ser Leu Ala Val Gly Gln Asn 
        115                 120                 125             


Trp Leu Leu Asp Glu Gln Thr Ser Thr Leu Thr Arg Leu Arg Tyr Ser 
    130                 135                 140                 


Tyr Arg Val Ile Cys Ser Asp Asn Tyr Tyr Gly Asp Asn Cys Ser Arg 
145                 150                 155                 160 


Leu Cys Lys Lys Arg Asn Asp Tyr Phe Gly His Tyr Val Cys Gln Pro 
                165                 170                 175     


Asp Gly Asn Pro Ser Cys Leu Pro Gly Trp Thr Gly Glu Tyr Cys Gln 
            180                 185                 190         


Gln Pro Ile Cys Leu Ser Gly Cys His Glu Gln Asn Gly Tyr Cys Ser 
        195                 200                 205             


Lys Pro Ala Glu Cys Leu Cys Arg Pro Gly Trp Gln Gly Arg Leu Cys 
    210                 215                 220                 


Asn Glu Cys Ile Pro His Asn Gly Cys Arg His Gly Thr Cys Ser Thr 
225                 230                 235                 240 


Pro Trp Gln Cys Thr Cys Asp Glu Gly Trp Gly Gly Leu Phe Cys Asp 
                245                 250                 255     


Gln Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
            260                 265                 270         


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
        275                 280                 285             


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
    290                 295                 300                 


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
305                 310                 315                 320 


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
                325                 330                 335     


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
            340                 345                 350         


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
        355                 360                 365             


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
    370                 375                 380                 


Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
385                 390                 395                 400 


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
                405                 410                 415     


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
            420                 425                 430         


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
        435                 440                 445             


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
    450                 455                 460                 


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
465                 470                 475                 480 


Ser Pro Gly Lys 
                


<210>  95
<211>  725
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DLL4-Fc Fusion 6

<400>  95

Ser Ser Val Phe Gln Leu Gln Leu Gln Glu Phe Ile Asn Glu Arg Gly 
1               5                   10                  15      


Val Leu Ala Ser Gly Arg Pro Cys Glu Pro Gly Cys Arg Thr Phe Phe 
            20                  25                  30          


Arg Val Cys Leu Lys His Phe Gln Ala Val Val Ser Pro Gly Pro Cys 
        35                  40                  45              


Thr Phe Gly Thr Val Ser Thr Pro Val Leu Gly Thr Asn Ser Phe Ala 
    50                  55                  60                  


Val Arg Asp Asp Ser Ser Gly Gly Gly Arg Asn Pro Leu Gln Leu Pro 
65                  70                  75                  80  


Leu Asn Phe Thr Trp Pro Gly Thr Phe Ser Leu Ile Ile Glu Ala Trp 
                85                  90                  95      


His Ala Pro Gly Asp Asp Leu Arg Pro Glu Ala Leu Pro Pro Asp Ala 
            100                 105                 110         


Leu Ile Ser Lys Phe Ala Ile Gln Gly Ser Leu Ala Val Gly Gln Asn 
        115                 120                 125             


Trp Leu Leu Asp Glu Gln Thr Ser Thr Leu Thr Arg Leu Arg Tyr Ser 
    130                 135                 140                 


Tyr Arg Val Ile Cys Ser Asp Asn Tyr Tyr Gly Asp Asn Cys Ser Arg 
145                 150                 155                 160 


Leu Cys Lys Lys Arg Asn Asp Tyr Phe Gly His Tyr Val Cys Gln Pro 
                165                 170                 175     


Asp Gly Asn Pro Ser Cys Leu Pro Gly Trp Thr Gly Glu Tyr Cys Gln 
            180                 185                 190         


Gln Pro Ile Cys Leu Ser Gly Cys His Glu Gln Asn Gly Tyr Cys Ser 
        195                 200                 205             


Lys Pro Ala Glu Cys Leu Cys Arg Pro Gly Trp Gln Gly Arg Leu Cys 
    210                 215                 220                 


Asn Glu Cys Ile Pro His Asn Gly Cys Arg His Gly Thr Cys Ser Thr 
225                 230                 235                 240 


Pro Trp Gln Cys Thr Cys Asp Glu Gly Trp Gly Gly Leu Phe Cys Asp 
                245                 250                 255     


Gln Asp Leu Asn Tyr Cys Thr His His Ser Pro Cys Lys Asn Gly Ala 
            260                 265                 270         


Thr Cys Ser Asn Ser Gly Gln Arg Ser Tyr Thr Cys Thr Cys Arg Pro 
        275                 280                 285             


Gly Tyr Thr Gly Val Asp Cys Glu Leu Glu Leu Ser Glu Cys Asp Ser 
    290                 295                 300                 


Asn Pro Cys Arg Asn Gly Gly Ser Cys Lys Asp Gln Glu Asp Gly Tyr 
305                 310                 315                 320 


His Cys Leu Cys Pro Pro Gly Tyr Tyr Gly Leu His Cys Glu His Ser 
                325                 330                 335     


Thr Leu Ser Cys Ala Asp Ser Pro Cys Phe Asn Gly Gly Ser Cys Arg 
            340                 345                 350         


Glu Arg Asn Gln Gly Ala Asn Tyr Ala Cys Glu Cys Pro Pro Asn Phe 
        355                 360                 365             


Thr Gly Ser Asn Cys Glu Lys Lys Val Asp Arg Cys Thr Ser Asn Pro 
    370                 375                 380                 


Cys Ala Asn Gly Gly Gln Cys Leu Asn Arg Gly Pro Ser Arg Met Cys 
385                 390                 395                 400 


Arg Cys Arg Pro Gly Phe Thr Gly Thr Tyr Cys Glu Leu His Val Ser 
                405                 410                 415     


Asp Cys Ala Arg Asn Pro Cys Ala His Gly Gly Thr Cys His Asp Leu 
            420                 425                 430         


Glu Asn Gly Leu Met Cys Thr Cys Pro Ala Gly Phe Ser Gly Arg Arg 
        435                 440                 445             


Cys Glu Val Arg Thr Ser Ile Asp Ala Cys Ala Ser Ser Pro Cys Phe 
    450                 455                 460                 


Asn Arg Ala Thr Cys Tyr Thr Asp Leu Ser Thr Asp Thr Phe Val Cys 
465                 470                 475                 480 


Asn Cys Pro Tyr Gly Phe Val Gly Ser Arg Cys Glu Phe Pro Val Gly 
                485                 490                 495     


Leu Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
            500                 505                 510         


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
        515                 520                 525             


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
    530                 535                 540                 


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
545                 550                 555                 560 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
                565                 570                 575     


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
            580                 585                 590         


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
        595                 600                 605             


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
    610                 615                 620                 


Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln 
625                 630                 635                 640 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
                645                 650                 655     


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
            660                 665                 670         


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
        675                 680                 685             


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
    690                 695                 700                 


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
705                 710                 715                 720 


Leu Ser Pro Gly Lys 
                725 


<210>  96
<211>  376
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Thymidine kinase

<400>  96

Met Ala Ser Tyr Pro Cys His Gln His Ala Ser Ala Phe Asp Gln Ala 
1               5                   10                  15      


Ala Arg Ser Arg Gly His Ser Asn Arg Arg Thr Ala Leu Arg Pro Arg 
            20                  25                  30          


Arg Gln Gln Glu Ala Thr Glu Val Arg Leu Glu Gln Lys Met Pro Thr 
        35                  40                  45              


Leu Leu Arg Val Tyr Ile Asp Gly Pro His Gly Met Gly Lys Thr Thr 
    50                  55                  60                  


Thr Thr Gln Leu Leu Val Ala Leu Gly Ser Arg Asp Asp Ile Val Tyr 
65                  70                  75                  80  


Val Pro Glu Pro Met Thr Tyr Trp Gln Val Leu Gly Ala Ser Glu Thr 
                85                  90                  95      


Ile Ala Asn Ile Tyr Thr Thr Gln His Arg Leu Asp Gln Gly Glu Ile 
            100                 105                 110         


Ser Ala Gly Asp Ala Ala Val Val Met Thr Ser Ala Gln Ile Thr Met 
        115                 120                 125             


Gly Met Pro Tyr Ala Val Thr Asp Ala Val Leu Ala Pro His Ile Gly 
    130                 135                 140                 


Gly Glu Ala Gly Ser Ser His Ala Pro Pro Pro Ala Leu Thr Leu Ile 
145                 150                 155                 160 


Phe Asp Arg His Pro Ile Ala Ala Leu Leu Cys Tyr Pro Ala Ala Arg 
                165                 170                 175     


Tyr Leu Met Gly Ser Met Thr Pro Gln Ala Val Leu Ala Phe Val Ala 
            180                 185                 190         


Leu Ile Pro Pro Thr Leu Pro Gly Thr Asn Ile Val Leu Gly Ala Leu 
        195                 200                 205             


Pro Glu Asp Arg His Ile Asp Arg Leu Ala Lys Arg Gln Arg Pro Gly 
    210                 215                 220                 


Glu Arg Leu Asp Leu Ala Met Leu Ala Ala Ile Arg Arg Val Tyr Gly 
225                 230                 235                 240 


Leu Leu Ala Asn Thr Val Arg Tyr Leu Gln Gly Gly Gly Ser Trp Arg 
                245                 250                 255     


Glu Asp Trp Gly Gln Leu Ser Gly Thr Ala Val Pro Pro Gln Gly Ala 
            260                 265                 270         


Glu Pro Gln Ser Asn Ala Gly Pro Arg Pro His Ile Gly Asp Thr Leu 
        275                 280                 285             


Phe Thr Leu Phe Arg Ala Pro Glu Leu Leu Ala Pro Asn Gly Asp Leu 
    290                 295                 300                 


Tyr Asn Val Phe Ala Trp Ala Leu Asp Val Leu Ala Lys Arg Leu Arg 
305                 310                 315                 320 


Pro Met His Val Phe Ile Leu Asp Tyr Asp Gln Ser Pro Ala Gly Cys 
                325                 330                 335     


Arg Asp Ala Leu Leu Gln Leu Thr Ser Gly Met Val Gln Thr His Val 
            340                 345                 350         


Thr Thr Pro Gly Ser Ile Pro Thr Ile Cys Asp Leu Ala Arg Thr Phe 
        355                 360                 365             


Ala Arg Glu Met Gly Glu Ala Asn 
    370                 375     


<210>  97
<211>  741
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated PSMA

<400>  97

Met Trp Asn Leu Leu Ala Arg Arg Pro Arg Trp Leu Cys Ala Gly Ala 
1               5                   10                  15      


Leu Val Leu Ala Gly Gly Phe Phe Leu Leu Gly Phe Leu Phe Gly Trp 
            20                  25                  30          


Phe Ile Lys Ser Ser Asn Glu Ala Thr Asn Ile Thr Pro Lys His Asn 
        35                  40                  45              


Met Lys Ala Phe Leu Asp Glu Leu Lys Ala Glu Asn Ile Lys Lys Phe 
    50                  55                  60                  


Leu Tyr Asn Phe Thr Gln Ile Pro His Leu Ala Gly Thr Glu Gln Asn 
65                  70                  75                  80  


Phe Gln Leu Ala Lys Gln Ile Gln Ser Gln Trp Lys Glu Phe Gly Leu 
                85                  90                  95      


Asp Ser Val Glu Leu Ala His Tyr Asp Val Leu Leu Ser Tyr Pro Asn 
            100                 105                 110         


Lys Thr His Pro Asn Tyr Ile Ser Ile Ile Asn Glu Asp Gly Asn Glu 
        115                 120                 125             


Ile Phe Asn Thr Ser Leu Phe Glu Pro Pro Pro Pro Gly Tyr Glu Asn 
    130                 135                 140                 


Val Ser Asp Ile Val Pro Pro Phe Ser Ala Phe Ser Pro Gln Gly Met 
145                 150                 155                 160 


Pro Glu Gly Asp Leu Val Tyr Val Asn Tyr Ala Arg Thr Glu Asp Phe 
                165                 170                 175     


Phe Lys Leu Glu Arg Asp Met Lys Ile Asn Cys Ser Gly Lys Ile Val 
            180                 185                 190         


Ile Ala Arg Tyr Gly Lys Val Phe Arg Gly Asn Lys Val Lys Asn Ala 
        195                 200                 205             


Gln Leu Ala Gly Ala Lys Gly Val Ile Leu Tyr Ser Asp Pro Ala Asp 
    210                 215                 220                 


Tyr Phe Ala Pro Gly Val Lys Ser Tyr Pro Asp Gly Trp Asn Leu Pro 
225                 230                 235                 240 


Gly Gly Gly Val Gln Arg Gly Asn Ile Leu Asn Leu Asn Gly Ala Gly 
                245                 250                 255     


Asp Pro Leu Thr Pro Gly Tyr Pro Ala Asn Glu Tyr Ala Tyr Arg Arg 
            260                 265                 270         


Gly Ile Ala Glu Ala Val Gly Leu Pro Ser Ile Pro Val His Pro Ile 
        275                 280                 285             


Gly Tyr Tyr Asp Ala Gln Lys Leu Leu Glu Lys Met Gly Gly Ser Ala 
    290                 295                 300                 


Pro Pro Asp Ser Ser Trp Arg Gly Ser Leu Lys Val Pro Tyr Asn Val 
305                 310                 315                 320 


Gly Pro Gly Phe Thr Gly Asn Phe Ser Thr Gln Lys Val Lys Met His 
                325                 330                 335     


Ile His Ser Thr Asn Glu Val Thr Arg Ile Tyr Asn Val Ile Gly Thr 
            340                 345                 350         


Leu Arg Gly Ala Val Glu Pro Asp Arg Tyr Val Ile Leu Gly Gly His 
        355                 360                 365             


Arg Asp Ser Trp Val Phe Gly Gly Ile Asp Pro Gln Ser Gly Ala Ala 
    370                 375                 380                 


Val Val His Glu Ile Val Arg Ser Phe Gly Thr Leu Lys Lys Glu Gly 
385                 390                 395                 400 


Trp Arg Pro Arg Arg Thr Ile Leu Phe Ala Ser Trp Asp Ala Glu Glu 
                405                 410                 415     


Phe Gly Leu Leu Gly Ser Thr Glu Trp Ala Glu Glu Asn Ser Arg Leu 
            420                 425                 430         


Leu Gln Glu Arg Gly Val Ala Tyr Ile Asn Ala Asp Ser Ser Ile Glu 
        435                 440                 445             


Gly Asn Tyr Thr Leu Arg Val Asp Cys Thr Pro Leu Met Tyr Ser Leu 
    450                 455                 460                 


Val His Asn Leu Thr Lys Glu Leu Lys Ser Pro Asp Glu Gly Phe Glu 
465                 470                 475                 480 


Gly Lys Ser Leu Tyr Glu Ser Trp Thr Lys Lys Ser Pro Ser Pro Glu 
                485                 490                 495     


Phe Ser Gly Met Pro Arg Ile Ser Lys Leu Gly Ser Gly Asn Asp Phe 
            500                 505                 510         


Glu Val Phe Phe Gln Arg Leu Gly Ile Ala Ser Gly Arg Ala Arg Tyr 
        515                 520                 525             


Thr Lys Asn Trp Glu Thr Asn Lys Phe Ser Gly Tyr Pro Leu Tyr His 
    530                 535                 540                 


Ser Val Tyr Glu Thr Tyr Glu Leu Val Glu Lys Phe Tyr Asp Pro Met 
545                 550                 555                 560 


Phe Lys Tyr His Leu Thr Val Ala Gln Val Arg Gly Gly Met Val Phe 
                565                 570                 575     


Glu Leu Ala Asn Ser Ile Val Leu Pro Phe Asp Cys Arg Asp Tyr Ala 
            580                 585                 590         


Val Val Leu Arg Lys Tyr Ala Asp Lys Ile Tyr Ser Ile Ser Met Lys 
        595                 600                 605             


His Pro Gln Glu Met Lys Thr Tyr Ser Val Ser Phe Asp Ser Leu Phe 
    610                 615                 620                 


Ser Ala Val Lys Asn Phe Thr Glu Ile Ala Ser Lys Phe Ser Glu Arg 
625                 630                 635                 640 


Leu Gln Asp Phe Asp Lys Ser Asn Pro Ile Val Leu Arg Met Met Asn 
                645                 650                 655     


Asp Gln Leu Met Phe Leu Glu Arg Ala Phe Ile Asp Pro Leu Gly Leu 
            660                 665                 670         


Pro Asp Arg Pro Phe Tyr Arg His Val Ile Tyr Ala Pro Ser Ser His 
        675                 680                 685             


Asn Lys Tyr Ala Gly Glu Ser Phe Pro Gly Ile Tyr Asp Ala Leu Phe 
    690                 695                 700                 


Asp Ile Glu Ser Lys Val Asp Pro Ser Lys Ala Trp Gly Glu Val Lys 
705                 710                 715                 720 


Arg Gln Ile Tyr Val Ala Ala Phe Thr Val Gln Ala Ala Ala Glu Thr 
                725                 730                 735     


Leu Ser Glu Val Ala 
            740     


<210>  98
<211>  1135
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HSV-TK-PSMA fusion

<400>  98

Met Ala Ser Tyr Pro Cys His Gln His Ala Ser Ala Phe Asp Gln Ala 
1               5                   10                  15      


Ala Arg Ser Arg Gly His Ser Asn Arg Arg Thr Ala Leu Arg Pro Arg 
            20                  25                  30          


Arg Gln Gln Glu Ala Thr Glu Val Arg Leu Glu Gln Lys Met Pro Thr 
        35                  40                  45              


Leu Leu Arg Val Tyr Ile Asp Gly Pro His Gly Met Gly Lys Thr Thr 
    50                  55                  60                  


Thr Thr Gln Leu Leu Val Ala Leu Gly Ser Arg Asp Asp Ile Val Tyr 
65                  70                  75                  80  


Val Pro Glu Pro Met Thr Tyr Trp Gln Val Leu Gly Ala Ser Glu Thr 
                85                  90                  95      


Ile Ala Asn Ile Tyr Thr Thr Gln His Arg Leu Asp Gln Gly Glu Ile 
            100                 105                 110         


Ser Ala Gly Asp Ala Ala Val Val Met Thr Ser Ala Gln Ile Thr Met 
        115                 120                 125             


Gly Met Pro Tyr Ala Val Thr Asp Ala Val Leu Ala Pro His Ile Gly 
    130                 135                 140                 


Gly Glu Ala Gly Ser Ser His Ala Pro Pro Pro Ala Leu Thr Leu Ile 
145                 150                 155                 160 


Phe Asp Arg His Pro Ile Ala Ala Leu Leu Cys Tyr Pro Ala Ala Arg 
                165                 170                 175     


Tyr Leu Met Gly Ser Met Thr Pro Gln Ala Val Leu Ala Phe Val Ala 
            180                 185                 190         


Leu Ile Pro Pro Thr Leu Pro Gly Thr Asn Ile Val Leu Gly Ala Leu 
        195                 200                 205             


Pro Glu Asp Arg His Ile Asp Arg Leu Ala Lys Arg Gln Arg Pro Gly 
    210                 215                 220                 


Glu Arg Leu Asp Leu Ala Met Leu Ala Ala Ile Arg Arg Val Tyr Gly 
225                 230                 235                 240 


Leu Leu Ala Asn Thr Val Arg Tyr Leu Gln Gly Gly Gly Ser Trp Arg 
                245                 250                 255     


Glu Asp Trp Gly Gln Leu Ser Gly Thr Ala Val Pro Pro Gln Gly Ala 
            260                 265                 270         


Glu Pro Gln Ser Asn Ala Gly Pro Arg Pro His Ile Gly Asp Thr Leu 
        275                 280                 285             


Phe Thr Leu Phe Arg Ala Pro Glu Leu Leu Ala Pro Asn Gly Asp Leu 
    290                 295                 300                 


Tyr Asn Val Phe Ala Trp Ala Leu Asp Val Leu Ala Lys Arg Leu Arg 
305                 310                 315                 320 


Pro Met His Val Phe Ile Leu Asp Tyr Asp Gln Ser Pro Ala Gly Cys 
                325                 330                 335     


Arg Asp Ala Leu Leu Gln Leu Thr Ser Gly Met Val Gln Thr His Val 
            340                 345                 350         


Thr Thr Pro Gly Ser Ile Pro Thr Ile Cys Asp Leu Ala Arg Thr Phe 
        355                 360                 365             


Ala Arg Glu Met Gly Glu Ala Asn Gly Ser Thr Ser Gly Ser Gly Lys 
    370                 375                 380                 


Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Met Trp Asn Leu Leu Ala 
385                 390                 395                 400 


Arg Arg Pro Arg Trp Leu Cys Ala Gly Ala Leu Val Leu Ala Gly Gly 
                405                 410                 415     


Phe Phe Leu Leu Gly Phe Leu Phe Gly Trp Phe Ile Lys Ser Ser Asn 
            420                 425                 430         


Glu Ala Thr Asn Ile Thr Pro Lys His Asn Met Lys Ala Phe Leu Asp 
        435                 440                 445             


Glu Leu Lys Ala Glu Asn Ile Lys Lys Phe Leu Tyr Asn Phe Thr Gln 
    450                 455                 460                 


Ile Pro His Leu Ala Gly Thr Glu Gln Asn Phe Gln Leu Ala Lys Gln 
465                 470                 475                 480 


Ile Gln Ser Gln Trp Lys Glu Phe Gly Leu Asp Ser Val Glu Leu Ala 
                485                 490                 495     


His Tyr Asp Val Leu Leu Ser Tyr Pro Asn Lys Thr His Pro Asn Tyr 
            500                 505                 510         


Ile Ser Ile Ile Asn Glu Asp Gly Asn Glu Ile Phe Asn Thr Ser Leu 
        515                 520                 525             


Phe Glu Pro Pro Pro Pro Gly Tyr Glu Asn Val Ser Asp Ile Val Pro 
    530                 535                 540                 


Pro Phe Ser Ala Phe Ser Pro Gln Gly Met Pro Glu Gly Asp Leu Val 
545                 550                 555                 560 


Tyr Val Asn Tyr Ala Arg Thr Glu Asp Phe Phe Lys Leu Glu Arg Asp 
                565                 570                 575     


Met Lys Ile Asn Cys Ser Gly Lys Ile Val Ile Ala Arg Tyr Gly Lys 
            580                 585                 590         


Val Phe Arg Gly Asn Lys Val Lys Asn Ala Gln Leu Ala Gly Ala Lys 
        595                 600                 605             


Gly Val Ile Leu Tyr Ser Asp Pro Ala Asp Tyr Phe Ala Pro Gly Val 
    610                 615                 620                 


Lys Ser Tyr Pro Asp Gly Trp Asn Leu Pro Gly Gly Gly Val Gln Arg 
625                 630                 635                 640 


Gly Asn Ile Leu Asn Leu Asn Gly Ala Gly Asp Pro Leu Thr Pro Gly 
                645                 650                 655     


Tyr Pro Ala Asn Glu Tyr Ala Tyr Arg Arg Gly Ile Ala Glu Ala Val 
            660                 665                 670         


Gly Leu Pro Ser Ile Pro Val His Pro Ile Gly Tyr Tyr Asp Ala Gln 
        675                 680                 685             


Lys Leu Leu Glu Lys Met Gly Gly Ser Ala Pro Pro Asp Ser Ser Trp 
    690                 695                 700                 


Arg Gly Ser Leu Lys Val Pro Tyr Asn Val Gly Pro Gly Phe Thr Gly 
705                 710                 715                 720 


Asn Phe Ser Thr Gln Lys Val Lys Met His Ile His Ser Thr Asn Glu 
                725                 730                 735     


Val Thr Arg Ile Tyr Asn Val Ile Gly Thr Leu Arg Gly Ala Val Glu 
            740                 745                 750         


Pro Asp Arg Tyr Val Ile Leu Gly Gly His Arg Asp Ser Trp Val Phe 
        755                 760                 765             


Gly Gly Ile Asp Pro Gln Ser Gly Ala Ala Val Val His Glu Ile Val 
    770                 775                 780                 


Arg Ser Phe Gly Thr Leu Lys Lys Glu Gly Trp Arg Pro Arg Arg Thr 
785                 790                 795                 800 


Ile Leu Phe Ala Ser Trp Asp Ala Glu Glu Phe Gly Leu Leu Gly Ser 
                805                 810                 815     


Thr Glu Trp Ala Glu Glu Asn Ser Arg Leu Leu Gln Glu Arg Gly Val 
            820                 825                 830         


Ala Tyr Ile Asn Ala Asp Ser Ser Ile Glu Gly Asn Tyr Thr Leu Arg 
        835                 840                 845             


Val Asp Cys Thr Pro Leu Met Tyr Ser Leu Val His Asn Leu Thr Lys 
    850                 855                 860                 


Glu Leu Lys Ser Pro Asp Glu Gly Phe Glu Gly Lys Ser Leu Tyr Glu 
865                 870                 875                 880 


Ser Trp Thr Lys Lys Ser Pro Ser Pro Glu Phe Ser Gly Met Pro Arg 
                885                 890                 895     


Ile Ser Lys Leu Gly Ser Gly Asn Asp Phe Glu Val Phe Phe Gln Arg 
            900                 905                 910         


Leu Gly Ile Ala Ser Gly Arg Ala Arg Tyr Thr Lys Asn Trp Glu Thr 
        915                 920                 925             


Asn Lys Phe Ser Gly Tyr Pro Leu Tyr His Ser Val Tyr Glu Thr Tyr 
    930                 935                 940                 


Glu Leu Val Glu Lys Phe Tyr Asp Pro Met Phe Lys Tyr His Leu Thr 
945                 950                 955                 960 


Val Ala Gln Val Arg Gly Gly Met Val Phe Glu Leu Ala Asn Ser Ile 
                965                 970                 975     


Val Leu Pro Phe Asp Cys Arg Asp Tyr Ala Val Val Leu Arg Lys Tyr 
            980                 985                 990         


Ala Asp Lys Ile Tyr Ser Ile Ser  Met Lys His Pro Gln  Glu Met Lys 
        995                 1000                 1005             


Thr Tyr  Ser Val Ser Phe Asp  Ser Leu Phe Ser Ala  Val Lys Asn 
    1010                 1015                 1020             


Phe Thr  Glu Ile Ala Ser Lys  Phe Ser Glu Arg Leu  Gln Asp Phe 
    1025                 1030                 1035             


Asp Lys  Ser Asn Pro Ile Val  Leu Arg Met Met Asn  Asp Gln Leu 
    1040                 1045                 1050             


Met Phe  Leu Glu Arg Ala Phe  Ile Asp Pro Leu Gly  Leu Pro Asp 
    1055                 1060                 1065             


Arg Pro  Phe Tyr Arg His Val  Ile Tyr Ala Pro Ser  Ser His Asn 
    1070                 1075                 1080             


Lys Tyr  Ala Gly Glu Ser Phe  Pro Gly Ile Tyr Asp  Ala Leu Phe 
    1085                 1090                 1095             


Asp Ile  Glu Ser Lys Val Asp  Pro Ser Lys Ala Trp  Gly Glu Val 
    1100                 1105                 1110             


Lys Arg  Gln Ile Tyr Val Ala  Ala Phe Thr Val Gln  Ala Ala Ala 
    1115                 1120                 1125             


Glu Thr  Leu Ser Glu Val Ala  
    1130                 1135 


<210>  99
<211>  1139
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HSV-TK-T2A-PSMA

<400>  99

Met Ala Ser Tyr Pro Cys His Gln His Ala Ser Ala Phe Asp Gln Ala 
1               5                   10                  15      


Ala Arg Ser Arg Gly His Ser Asn Arg Arg Thr Ala Leu Arg Pro Arg 
            20                  25                  30          


Arg Gln Gln Glu Ala Thr Glu Val Arg Leu Glu Gln Lys Met Pro Thr 
        35                  40                  45              


Leu Leu Arg Val Tyr Ile Asp Gly Pro His Gly Met Gly Lys Thr Thr 
    50                  55                  60                  


Thr Thr Gln Leu Leu Val Ala Leu Gly Ser Arg Asp Asp Ile Val Tyr 
65                  70                  75                  80  


Val Pro Glu Pro Met Thr Tyr Trp Gln Val Leu Gly Ala Ser Glu Thr 
                85                  90                  95      


Ile Ala Asn Ile Tyr Thr Thr Gln His Arg Leu Asp Gln Gly Glu Ile 
            100                 105                 110         


Ser Ala Gly Asp Ala Ala Val Val Met Thr Ser Ala Gln Ile Thr Met 
        115                 120                 125             


Gly Met Pro Tyr Ala Val Thr Asp Ala Val Leu Ala Pro His Ile Gly 
    130                 135                 140                 


Gly Glu Ala Gly Ser Ser His Ala Pro Pro Pro Ala Leu Thr Leu Ile 
145                 150                 155                 160 


Phe Asp Arg His Pro Ile Ala Ala Leu Leu Cys Tyr Pro Ala Ala Arg 
                165                 170                 175     


Tyr Leu Met Gly Ser Met Thr Pro Gln Ala Val Leu Ala Phe Val Ala 
            180                 185                 190         


Leu Ile Pro Pro Thr Leu Pro Gly Thr Asn Ile Val Leu Gly Ala Leu 
        195                 200                 205             


Pro Glu Asp Arg His Ile Asp Arg Leu Ala Lys Arg Gln Arg Pro Gly 
    210                 215                 220                 


Glu Arg Leu Asp Leu Ala Met Leu Ala Ala Ile Arg Arg Val Tyr Gly 
225                 230                 235                 240 


Leu Leu Ala Asn Thr Val Arg Tyr Leu Gln Gly Gly Gly Ser Trp Arg 
                245                 250                 255     


Glu Asp Trp Gly Gln Leu Ser Gly Thr Ala Val Pro Pro Gln Gly Ala 
            260                 265                 270         


Glu Pro Gln Ser Asn Ala Gly Pro Arg Pro His Ile Gly Asp Thr Leu 
        275                 280                 285             


Phe Thr Leu Phe Arg Ala Pro Glu Leu Leu Ala Pro Asn Gly Asp Leu 
    290                 295                 300                 


Tyr Asn Val Phe Ala Trp Ala Leu Asp Val Leu Ala Lys Arg Leu Arg 
305                 310                 315                 320 


Pro Met His Val Phe Ile Leu Asp Tyr Asp Gln Ser Pro Ala Gly Cys 
                325                 330                 335     


Arg Asp Ala Leu Leu Gln Leu Thr Ser Gly Met Val Gln Thr His Val 
            340                 345                 350         


Thr Thr Pro Gly Ser Ile Pro Thr Ile Cys Asp Leu Ala Arg Thr Phe 
        355                 360                 365             


Ala Arg Glu Met Gly Glu Ala Asn Ser Gly Ser Gly Glu Gly Arg Gly 
    370                 375                 380                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Trp 
385                 390                 395                 400 


Asn Leu Leu Ala Arg Arg Pro Arg Trp Leu Cys Ala Gly Ala Leu Val 
                405                 410                 415     


Leu Ala Gly Gly Phe Phe Leu Leu Gly Phe Leu Phe Gly Trp Phe Ile 
            420                 425                 430         


Lys Ser Ser Asn Glu Ala Thr Asn Ile Thr Pro Lys His Asn Met Lys 
        435                 440                 445             


Ala Phe Leu Asp Glu Leu Lys Ala Glu Asn Ile Lys Lys Phe Leu Tyr 
    450                 455                 460                 


Asn Phe Thr Gln Ile Pro His Leu Ala Gly Thr Glu Gln Asn Phe Gln 
465                 470                 475                 480 


Leu Ala Lys Gln Ile Gln Ser Gln Trp Lys Glu Phe Gly Leu Asp Ser 
                485                 490                 495     


Val Glu Leu Ala His Tyr Asp Val Leu Leu Ser Tyr Pro Asn Lys Thr 
            500                 505                 510         


His Pro Asn Tyr Ile Ser Ile Ile Asn Glu Asp Gly Asn Glu Ile Phe 
        515                 520                 525             


Asn Thr Ser Leu Phe Glu Pro Pro Pro Pro Gly Tyr Glu Asn Val Ser 
    530                 535                 540                 


Asp Ile Val Pro Pro Phe Ser Ala Phe Ser Pro Gln Gly Met Pro Glu 
545                 550                 555                 560 


Gly Asp Leu Val Tyr Val Asn Tyr Ala Arg Thr Glu Asp Phe Phe Lys 
                565                 570                 575     


Leu Glu Arg Asp Met Lys Ile Asn Cys Ser Gly Lys Ile Val Ile Ala 
            580                 585                 590         


Arg Tyr Gly Lys Val Phe Arg Gly Asn Lys Val Lys Asn Ala Gln Leu 
        595                 600                 605             


Ala Gly Ala Lys Gly Val Ile Leu Tyr Ser Asp Pro Ala Asp Tyr Phe 
    610                 615                 620                 


Ala Pro Gly Val Lys Ser Tyr Pro Asp Gly Trp Asn Leu Pro Gly Gly 
625                 630                 635                 640 


Gly Val Gln Arg Gly Asn Ile Leu Asn Leu Asn Gly Ala Gly Asp Pro 
                645                 650                 655     


Leu Thr Pro Gly Tyr Pro Ala Asn Glu Tyr Ala Tyr Arg Arg Gly Ile 
            660                 665                 670         


Ala Glu Ala Val Gly Leu Pro Ser Ile Pro Val His Pro Ile Gly Tyr 
        675                 680                 685             


Tyr Asp Ala Gln Lys Leu Leu Glu Lys Met Gly Gly Ser Ala Pro Pro 
    690                 695                 700                 


Asp Ser Ser Trp Arg Gly Ser Leu Lys Val Pro Tyr Asn Val Gly Pro 
705                 710                 715                 720 


Gly Phe Thr Gly Asn Phe Ser Thr Gln Lys Val Lys Met His Ile His 
                725                 730                 735     


Ser Thr Asn Glu Val Thr Arg Ile Tyr Asn Val Ile Gly Thr Leu Arg 
            740                 745                 750         


Gly Ala Val Glu Pro Asp Arg Tyr Val Ile Leu Gly Gly His Arg Asp 
        755                 760                 765             


Ser Trp Val Phe Gly Gly Ile Asp Pro Gln Ser Gly Ala Ala Val Val 
    770                 775                 780                 


His Glu Ile Val Arg Ser Phe Gly Thr Leu Lys Lys Glu Gly Trp Arg 
785                 790                 795                 800 


Pro Arg Arg Thr Ile Leu Phe Ala Ser Trp Asp Ala Glu Glu Phe Gly 
                805                 810                 815     


Leu Leu Gly Ser Thr Glu Trp Ala Glu Glu Asn Ser Arg Leu Leu Gln 
            820                 825                 830         


Glu Arg Gly Val Ala Tyr Ile Asn Ala Asp Ser Ser Ile Glu Gly Asn 
        835                 840                 845             


Tyr Thr Leu Arg Val Asp Cys Thr Pro Leu Met Tyr Ser Leu Val His 
    850                 855                 860                 


Asn Leu Thr Lys Glu Leu Lys Ser Pro Asp Glu Gly Phe Glu Gly Lys 
865                 870                 875                 880 


Ser Leu Tyr Glu Ser Trp Thr Lys Lys Ser Pro Ser Pro Glu Phe Ser 
                885                 890                 895     


Gly Met Pro Arg Ile Ser Lys Leu Gly Ser Gly Asn Asp Phe Glu Val 
            900                 905                 910         


Phe Phe Gln Arg Leu Gly Ile Ala Ser Gly Arg Ala Arg Tyr Thr Lys 
        915                 920                 925             


Asn Trp Glu Thr Asn Lys Phe Ser Gly Tyr Pro Leu Tyr His Ser Val 
    930                 935                 940                 


Tyr Glu Thr Tyr Glu Leu Val Glu Lys Phe Tyr Asp Pro Met Phe Lys 
945                 950                 955                 960 


Tyr His Leu Thr Val Ala Gln Val Arg Gly Gly Met Val Phe Glu Leu 
                965                 970                 975     


Ala Asn Ser Ile Val Leu Pro Phe Asp Cys Arg Asp Tyr Ala Val Val 
            980                 985                 990         


Leu Arg Lys Tyr Ala Asp Lys Ile  Tyr Ser Ile Ser Met  Lys His Pro 
        995                 1000                 1005             


Gln Glu  Met Lys Thr Tyr Ser  Val Ser Phe Asp Ser  Leu Phe Ser 
    1010                 1015                 1020             


Ala Val  Lys Asn Phe Thr Glu  Ile Ala Ser Lys Phe  Ser Glu Arg 
    1025                 1030                 1035             


Leu Gln  Asp Phe Asp Lys Ser  Asn Pro Ile Val Leu  Arg Met Met 
    1040                 1045                 1050             


Asn Asp  Gln Leu Met Phe Leu  Glu Arg Ala Phe Ile  Asp Pro Leu 
    1055                 1060                 1065             


Gly Leu  Pro Asp Arg Pro Phe  Tyr Arg His Val Ile  Tyr Ala Pro 
    1070                 1075                 1080             


Ser Ser  His Asn Lys Tyr Ala  Gly Glu Ser Phe Pro  Gly Ile Tyr 
    1085                 1090                 1095             


Asp Ala  Leu Phe Asp Ile Glu  Ser Lys Val Asp Pro  Ser Lys Ala 
    1100                 1105                 1110             


Trp Gly  Glu Val Lys Arg Gln  Ile Tyr Val Ala Ala  Phe Thr Val 
    1115                 1120                 1125             


Gln Ala  Ala Ala Glu Thr Leu  Ser Glu Val Ala 
    1130                 1135                 


<210>  100
<211>  843
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PSMA-T2A-CD24

<400>  100

Met Trp Asn Leu Leu Ala Arg Arg Pro Arg Trp Leu Cys Ala Gly Ala 
1               5                   10                  15      


Leu Val Leu Ala Gly Gly Phe Phe Leu Leu Gly Phe Leu Phe Gly Trp 
            20                  25                  30          


Phe Ile Lys Ser Ser Asn Glu Ala Thr Asn Ile Thr Pro Lys His Asn 
        35                  40                  45              


Met Lys Ala Phe Leu Asp Glu Leu Lys Ala Glu Asn Ile Lys Lys Phe 
    50                  55                  60                  


Leu Tyr Asn Phe Thr Gln Ile Pro His Leu Ala Gly Thr Glu Gln Asn 
65                  70                  75                  80  


Phe Gln Leu Ala Lys Gln Ile Gln Ser Gln Trp Lys Glu Phe Gly Leu 
                85                  90                  95      


Asp Ser Val Glu Leu Ala His Tyr Asp Val Leu Leu Ser Tyr Pro Asn 
            100                 105                 110         


Lys Thr His Pro Asn Tyr Ile Ser Ile Ile Asn Glu Asp Gly Asn Glu 
        115                 120                 125             


Ile Phe Asn Thr Ser Leu Phe Glu Pro Pro Pro Pro Gly Tyr Glu Asn 
    130                 135                 140                 


Val Ser Asp Ile Val Pro Pro Phe Ser Ala Phe Ser Pro Gln Gly Met 
145                 150                 155                 160 


Pro Glu Gly Asp Leu Val Tyr Val Asn Tyr Ala Arg Thr Glu Asp Phe 
                165                 170                 175     


Phe Lys Leu Glu Arg Asp Met Lys Ile Asn Cys Ser Gly Lys Ile Val 
            180                 185                 190         


Ile Ala Arg Tyr Gly Lys Val Phe Arg Gly Asn Lys Val Lys Asn Ala 
        195                 200                 205             


Gln Leu Ala Gly Ala Lys Gly Val Ile Leu Tyr Ser Asp Pro Ala Asp 
    210                 215                 220                 


Tyr Phe Ala Pro Gly Val Lys Ser Tyr Pro Asp Gly Trp Asn Leu Pro 
225                 230                 235                 240 


Gly Gly Gly Val Gln Arg Gly Asn Ile Leu Asn Leu Asn Gly Ala Gly 
                245                 250                 255     


Asp Pro Leu Thr Pro Gly Tyr Pro Ala Asn Glu Tyr Ala Tyr Arg Arg 
            260                 265                 270         


Gly Ile Ala Glu Ala Val Gly Leu Pro Ser Ile Pro Val His Pro Ile 
        275                 280                 285             


Gly Tyr Tyr Asp Ala Gln Lys Leu Leu Glu Lys Met Gly Gly Ser Ala 
    290                 295                 300                 


Pro Pro Asp Ser Ser Trp Arg Gly Ser Leu Lys Val Pro Tyr Asn Val 
305                 310                 315                 320 


Gly Pro Gly Phe Thr Gly Asn Phe Ser Thr Gln Lys Val Lys Met His 
                325                 330                 335     


Ile His Ser Thr Asn Glu Val Thr Arg Ile Tyr Asn Val Ile Gly Thr 
            340                 345                 350         


Leu Arg Gly Ala Val Glu Pro Asp Arg Tyr Val Ile Leu Gly Gly His 
        355                 360                 365             


Arg Asp Ser Trp Val Phe Gly Gly Ile Asp Pro Gln Ser Gly Ala Ala 
    370                 375                 380                 


Val Val His Glu Ile Val Arg Ser Phe Gly Thr Leu Lys Lys Glu Gly 
385                 390                 395                 400 


Trp Arg Pro Arg Arg Thr Ile Leu Phe Ala Ser Trp Asp Ala Glu Glu 
                405                 410                 415     


Phe Gly Leu Leu Gly Ser Thr Glu Trp Ala Glu Glu Asn Ser Arg Leu 
            420                 425                 430         


Leu Gln Glu Arg Gly Val Ala Tyr Ile Asn Ala Asp Ser Ser Ile Glu 
        435                 440                 445             


Gly Asn Tyr Thr Leu Arg Val Asp Cys Thr Pro Leu Met Tyr Ser Leu 
    450                 455                 460                 


Val His Asn Leu Thr Lys Glu Leu Lys Ser Pro Asp Glu Gly Phe Glu 
465                 470                 475                 480 


Gly Lys Ser Leu Tyr Glu Ser Trp Thr Lys Lys Ser Pro Ser Pro Glu 
                485                 490                 495     


Phe Ser Gly Met Pro Arg Ile Ser Lys Leu Gly Ser Gly Asn Asp Phe 
            500                 505                 510         


Glu Val Phe Phe Gln Arg Leu Gly Ile Ala Ser Gly Arg Ala Arg Tyr 
        515                 520                 525             


Thr Lys Asn Trp Glu Thr Asn Lys Phe Ser Gly Tyr Pro Leu Tyr His 
    530                 535                 540                 


Ser Val Tyr Glu Thr Tyr Glu Leu Val Glu Lys Phe Tyr Asp Pro Met 
545                 550                 555                 560 


Phe Lys Tyr His Leu Thr Val Ala Gln Val Arg Gly Gly Met Val Phe 
                565                 570                 575     


Glu Leu Ala Asn Ser Ile Val Leu Pro Phe Asp Cys Arg Asp Tyr Ala 
            580                 585                 590         


Val Val Leu Arg Lys Tyr Ala Asp Lys Ile Tyr Ser Ile Ser Met Lys 
        595                 600                 605             


His Pro Gln Glu Met Lys Thr Tyr Ser Val Ser Phe Asp Ser Leu Phe 
    610                 615                 620                 


Ser Ala Val Lys Asn Phe Thr Glu Ile Ala Ser Lys Phe Ser Glu Arg 
625                 630                 635                 640 


Leu Gln Asp Phe Asp Lys Ser Asn Pro Ile Val Leu Arg Met Met Asn 
                645                 650                 655     


Asp Gln Leu Met Phe Leu Glu Arg Ala Phe Ile Asp Pro Leu Gly Leu 
            660                 665                 670         


Pro Asp Arg Pro Phe Tyr Arg His Val Ile Tyr Ala Pro Ser Ser His 
        675                 680                 685             


Asn Lys Tyr Ala Gly Glu Ser Phe Pro Gly Ile Tyr Asp Ala Leu Phe 
    690                 695                 700                 


Asp Ile Glu Ser Lys Val Asp Pro Ser Lys Ala Trp Gly Glu Val Lys 
705                 710                 715                 720 


Arg Gln Ile Tyr Val Ala Ala Phe Thr Val Gln Ala Ala Ala Glu Thr 
                725                 730                 735     


Leu Ser Glu Val Ala Ser Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu 
            740                 745                 750         


Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Gly Arg Ala Met 
        755                 760                 765             


Val Ala Arg Leu Gly Leu Gly Leu Leu Leu Leu Ala Leu Leu Leu Pro 
    770                 775                 780                 


Thr Gln Ile Tyr Ser Ser Glu Thr Thr Thr Gly Thr Ser Ser Asn Ser 
785                 790                 795                 800 


Ser Gln Ser Thr Ser Asn Ser Gly Leu Ala Pro Asn Pro Thr Asn Ala 
                805                 810                 815     


Thr Thr Lys Ala Ala Gly Gly Ala Leu Gln Ser Thr Ala Ser Leu Phe 
            820                 825                 830         


Val Val Ser Leu Ser Leu Leu His Leu Tyr Ser 
        835                 840             


