                         SEQUENCE LISTING

<110>  The Board of Trustees of the University of Illinois
 
<120>  MULTIVALENT CHOLERA MULTIEPITOPE FUSION ANTIGEN (MEFA) AND 
       METHODS OF USE

<130>  7950-106164-02

<150>  US 63/171,410
<151>  2021-04-06

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  1134
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid sequence encoding a cholera MEFA fusion 
       protein

<400>  1
atgggcgcga ttaacgtgaa caccaacgtg agcgcgatga ccggcaaagt gagcgcggat       60

gaagcgaaaa acccgatgca gaaaagcatg gaacgcctga gcccggcgac cgcggatgcg      120

accgcggcga gcaaagcggc gggcctgcag attagcaacc gcctggcgga tagccgcccg      180

ccggatgaaa ttaaacagag caacgcgaac gatggcatta gcattgcgca gaccgcggaa      240

ggcgcgatga acgaaaccac caacattctg cagcgcatgc gcgatctggc gctgcagagc      300

agcaacggca gcaacagcag cagcgaacgc cgcgcgattc aggaaagcca gcatattgat      360

agccagaaaa aagcgcgcat tgcggaaacc accagctttg gcggcctgcc gagcgcgggc      420

cgcggcgtgt gctatgaagc gtttcagatt ggcgcggata gcggcgaagc ggtgatgctg      480

agcatgggca gcatgcgcag cgatacccag gcgatgggcg gcaaaagcta tcgcgcgcag      540

gaaggcaaaa tgcaggataa caccaacaac ggcagcggcg tgctgaccct gagctatacc      600

aacaaacagg gcgaagcgcg caccggcggc gtggaagtga gcggcgatgg cccgaaagaa      660

gaactggcga cctatattaa cggccagacc gaagatgtga aagcgagcgt gggcgaagat      720

ggcaaactgc tgcagagcca gagcgcgaac ggcagcaaca gcaaaagcga aattggcggc      780

ggcctgggcg gcgaaattgg ctttgatgcg ggccgcaacg tgaccgtggc ggatgtgaac      840

gtgagcaccg tggcgggcag ccaggaagcg gtgagcattc tggatggcgc gctgaaagcg      900

gtggatagcc agcgcgcgag cctgggcgcg tttcagaacc gctttggcca tgcgattagc      960

aacctggata acgtgaacga aaacgtgaac gcgagccgca gccgcattcg cgataccgat     1020

tatcagcatc tgaacagcat tctgctggtg accaaaattc tgcagcaggc gagcaccagc     1080

gtgctggcgc aggcgaaaca gagcccgagc gcggcgctga gcctgctggg ctaa           1134


<210>  2
<211>  377
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary cholera MEFA fusion protein

<400>  2

Met Gly Ala Ile Asn Val Asn Thr Asn Val Ser Ala Met Thr Gly Lys 
1               5                   10                  15      


Val Ser Ala Asp Glu Ala Lys Asn Pro Met Gln Lys Ser Met Glu Arg 
            20                  25                  30          


Leu Ser Pro Ala Thr Ala Asp Ala Thr Ala Ala Ser Lys Ala Ala Gly 
        35                  40                  45              


Leu Gln Ile Ser Asn Arg Leu Ala Asp Ser Arg Pro Pro Asp Glu Ile 
    50                  55                  60                  


Lys Gln Ser Asn Ala Asn Asp Gly Ile Ser Ile Ala Gln Thr Ala Glu 
65                  70                  75                  80  


Gly Ala Met Asn Glu Thr Thr Asn Ile Leu Gln Arg Met Arg Asp Leu 
                85                  90                  95      


Ala Leu Gln Ser Ser Asn Gly Ser Asn Ser Ser Ser Glu Arg Arg Ala 
            100                 105                 110         


Ile Gln Glu Ser Gln His Ile Asp Ser Gln Lys Lys Ala Arg Ile Ala 
        115                 120                 125             


Glu Thr Thr Ser Phe Gly Gly Leu Pro Ser Ala Gly Arg Gly Val Cys 
    130                 135                 140                 


Tyr Glu Ala Phe Gln Ile Gly Ala Asp Ser Gly Glu Ala Val Met Leu 
145                 150                 155                 160 


Ser Met Gly Ser Met Arg Ser Asp Thr Gln Ala Met Gly Gly Lys Ser 
                165                 170                 175     


Tyr Arg Ala Gln Glu Gly Lys Met Gln Asp Asn Thr Asn Asn Gly Ser 
            180                 185                 190         


Gly Val Leu Thr Leu Ser Tyr Thr Asn Lys Gln Gly Glu Ala Arg Thr 
        195                 200                 205             


Gly Gly Val Glu Val Ser Gly Asp Gly Pro Lys Glu Glu Leu Ala Thr 
    210                 215                 220                 


Tyr Ile Asn Gly Gln Thr Glu Asp Val Lys Ala Ser Val Gly Glu Asp 
225                 230                 235                 240 


Gly Lys Leu Leu Gln Ser Gln Ser Ala Asn Gly Ser Asn Ser Lys Ser 
                245                 250                 255     


Glu Ile Gly Gly Gly Leu Gly Gly Glu Ile Gly Phe Asp Ala Gly Arg 
            260                 265                 270         


Asn Val Thr Val Ala Asp Val Asn Val Ser Thr Val Ala Gly Ser Gln 
        275                 280                 285             


Glu Ala Val Ser Ile Leu Asp Gly Ala Leu Lys Ala Val Asp Ser Gln 
    290                 295                 300                 


Arg Ala Ser Leu Gly Ala Phe Gln Asn Arg Phe Gly His Ala Ile Ser 
305                 310                 315                 320 


Asn Leu Asp Asn Val Asn Glu Asn Val Asn Ala Ser Arg Ser Arg Ile 
                325                 330                 335     


Arg Asp Thr Asp Tyr Gln His Leu Asn Ser Ile Leu Leu Val Thr Lys 
            340                 345                 350         


Ile Leu Gln Gln Ala Ser Thr Ser Val Leu Ala Gln Ala Lys Gln Ser 
        355                 360                 365             


Pro Ser Ala Ala Leu Ser Leu Leu Gly 
    370                 375         


<210>  3
<211>  1134
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid sequence of a backbone protein


<220>
<221>  misc_feature
<222>  (43)..(75)
<223>  "N" is any nucleic acid

<220>
<221>  misc_feature
<222>  (103)..(135)
<223>  "N" is any nucleic acid

<220>
<221>  misc_feature
<222>  (166)..(201)
<223>  "N" is any nucleic acid

<220>
<221>  misc_feature
<222>  (301)..(333)
<223>  "N" is any nucleic acid

<220>
<221>  misc_feature
<222>  (346)..(375)
<223>  "N" is any nucleic acid

<220>
<221>  misc_feature
<222>  (406)..(441)
<223>  "N" is any nucleic acid

<220>
<221>  misc_feature
<222>  (550)..(582)
<223>  "N" is any nucleic acid

<220>
<221>  misc_feature
<222>  (622)..(657)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (730)..(772)
<223>  "N" is any nucleic acid

<220>
<221>  misc_feature
<222>  (1024)..(1056)
<223>  "N" is any nucleic acid

<400>  3
atgggcgcga ttaacgtgaa caccaacgtg agcgcgatga ccnnnnnnnn nnnnnnnnnn       60

nnnnnnnnnn nnnnnatgca gaaaagcatg gaacgcctga gcnnnnnnnn nnnnnnnnnn      120

nnnnnnnnnn nnnnngcggc gggcctgcag attagcaacc gcctgnnnnn nnnnnnnnnn      180

nnnnnnnnnn nnnnnnnnnn naacgcgaac gatggcatta gcattgcgca gaccgcggaa      240

ggcgcgatga acgaaaccac caacattctg cagcgcatgc gcgatctggc gctgcagagc      300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgattc aggaannnnn nnnnnnnnnn      360

nnnnnnnnnn nnnnncgcat tgcggaaacc accagctttg gcggcnnnnn nnnnnnnnnn      420

nnnnnnnnnn nnnnnnnnnn ntttcagatt ggcgcggata gcggcgaagc ggtgatgctg      480

agcatgggca gcatgcgcag cgatacccag gcgatgggcg gcaaaagcta tcgcgcgcag      540

gaaggcaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctgaccct gagctatacc      600

aacaaacagg gcgaagcgcg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa      660

gaactggcga cctatattaa cggccagacc gaagatgtga aagcgagcgt gggcgaagat      720

ggcaaactgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattggcggc      780

ggcctgggcg gcgaaattgg ctttgatgcg ggccgcaacg tgaccgtggc ggatgtgaac      840

gtgagcaccg tggcgggcag ccaggaagcg gtgagcattc tggatggcgc gctgaaagcg      900

gtggatagcc agcgcgcgag cctgggcgcg tttcagaacc gctttggcca tgcgattagc      960

aacctggata acgtgaacga aaacgtgaac gcgagccgca gccgcattcg cgataccgat     1020

tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnattc tgcagcaggc gagcaccagc     1080

gtgctggcgc aggcgaaaca gagcccgagc gcggcgctga gcctgctggg ctaa           1134


<210>  4
<211>  377
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary backbone protein


<220>
<221>  MISC_FEATURE
<222>  (15)..(25)
<223>  "Xaa" is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (35)..(45)
<223>  "Xaa" is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (56)..(67)
<223>  "Xaa" is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (101)..(111)
<223>  "Xaa" is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (116)..(125)
<223>  "Xaa" is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (136)..(147)
<223>  "Xaa" is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (184)..(194)
<223>  "Xaa" is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (208)..(219)
<223>  "Xaa" is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (244)..(257)
<223>  "Xaa" is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (342)..(352)
<223>  "Xaa" is any amino acid

<400>  4

Met Gly Ala Ile Asn Val Asn Thr Asn Val Ser Ala Met Thr Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Met Gln Lys Ser Met Glu Arg 
            20                  25                  30          


Leu Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ala Ala Gly 
        35                  40                  45              


Leu Gln Ile Ser Asn Arg Leu Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    50                  55                  60                  


Xaa Xaa Xaa Asn Ala Asn Asp Gly Ile Ser Ile Ala Gln Thr Ala Glu 
65                  70                  75                  80  


Gly Ala Met Asn Glu Thr Thr Asn Ile Leu Gln Arg Met Arg Asp Leu 
                85                  90                  95      


Ala Leu Gln Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ala 
            100                 105                 110         


Ile Gln Glu Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Arg Ile Ala 
        115                 120                 125             


Glu Thr Thr Ser Phe Gly Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    130                 135                 140                 


Xaa Xaa Xaa Phe Gln Ile Gly Ala Asp Ser Gly Glu Ala Val Met Leu 
145                 150                 155                 160 


Ser Met Gly Ser Met Arg Ser Asp Thr Gln Ala Met Gly Gly Lys Ser 
                165                 170                 175     


Tyr Arg Ala Gln Glu Gly Lys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            180                 185                 190         


Xaa Xaa Leu Thr Leu Ser Tyr Thr Asn Lys Gln Gly Glu Ala Arg Xaa 
        195                 200                 205             


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Glu Glu Leu Ala Thr 
    210                 215                 220                 


Tyr Ile Asn Gly Gln Thr Glu Asp Val Lys Ala Ser Val Gly Glu Asp 
225                 230                 235                 240 


Gly Lys Leu Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
                245                 250                 255     


Xaa Ile Gly Gly Gly Leu Gly Gly Glu Ile Gly Phe Asp Ala Gly Arg 
            260                 265                 270         


Asn Val Thr Val Ala Asp Val Asn Val Ser Thr Val Ala Gly Ser Gln 
        275                 280                 285             


Glu Ala Val Ser Ile Leu Asp Gly Ala Leu Lys Ala Val Asp Ser Gln 
    290                 295                 300                 


Arg Ala Ser Leu Gly Ala Phe Gln Asn Arg Phe Gly His Ala Ile Ser 
305                 310                 315                 320 


Asn Leu Asp Asn Val Asn Glu Asn Val Asn Ala Ser Arg Ser Arg Ile 
                325                 330                 335     


Arg Asp Thr Asp Tyr Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            340                 345                 350         


Ile Leu Gln Gln Ala Ser Thr Ser Val Leu Ala Gln Ala Lys Gln Ser 
        355                 360                 365             


Pro Ser Ala Ala Leu Ser Leu Leu Gly 
    370                 375         


<210>  5
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid encoding a TcpA epitope

<400>  5
ggcaaagtga gcgcggatga agcgaaaaac ccg                                    33


<210>  6
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary TcpA epitope

<400>  6

Gly Lys Val Ser Ala Asp Glu Ala Lys Asn Pro 
1               5                   10      


<210>  7
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid sequence encoding a TcpA epitope

<400>  7
ccggcgaccg cggatgcgac cgcggcgagc aaa                                    33


<210>  8
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary TcpA epitope

<400>  8

Pro Ala Thr Ala Asp Ala Thr Ala Ala Ser Lys 
1               5                   10      


<210>  9
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid sequence encoding a CTA epitope

<400>  9
gcggatagcc gcccgccgga tgaaattaaa cagagc                                 36


<210>  10
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary CTA epitope

<400>  10

Ala Asp Ser Arg Pro Pro Asp Glu Ile Lys Gln Ser 
1               5                   10          


<210>  11
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid sequence encoding a FlaB epitope

<400>  11
agcaacggca gcaacagcag cagcgaacgc cgc                                    33


<210>  12
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary FlaB epitope

<400>  12

Ser Asn Gly Ser Asn Ser Ser Ser Glu Arg Arg 
1               5                   10      


<210>  13
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid sequence encoding a CTB epitope

<400>  13
agccagcata ttgatagcca gaaaaaagcg                                        30


<210>  14
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary CTB epitope

<400>  14

Ser Gln His Ile Asp Ser Gln Lys Lys Ala 
1               5                   10  


<210>  15
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid sequence encoding an LPS O-antigen mimic 
       epitope

<400>  15
ctgccgagcg cgggccgcgg cgtgtgctat gaagcg                                 36


<210>  16
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary LPS O-antigen mimic epitope

<400>  16

Leu Pro Ser Ala Gly Arg Gly Val Cys Tyr Glu Ala 
1               5                   10          


<210>  17
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid sequence encoding a sialidase epitope

<400>  17
atgcaggata acaccaacaa cggcagcggc gtg                                    33


<210>  18
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary sialidase epitope

<400>  18

Met Gln Asp Asn Thr Asn Asn Gly Ser Gly Val 
1               5                   10      


<210>  19
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid sequence encoding a HlyA epitope

<400>  19
accggcggcg tggaagtgag cggcgatggc ccgaaa                                 36


<210>  20
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary HlyA epitope

<400>  20

Thr Gly Gly Val Glu Val Ser Gly Asp Gly Pro Lys 
1               5                   10          


<210>  21
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid sequence encoding a FlaC/D epitope

<400>  21
ctgcagagcc agagcgcgaa cggcagcaac agcaaaagcg aa                          42


<210>  22
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary FlaC/D epitope

<400>  22

Leu Gln Ser Gln Ser Ala Asn Gly Ser Asn Ser Lys Ser Glu 
1               5                   10                  


<210>  23
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid sequence encoding an LPS O-antigen mimic 
       epitope

<400>  23
cagcatctga acagcattct gctggtgacc aaa                                    33


<210>  24
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary LPS O-anitgen mimic epitope

<400>  24

Gln His Leu Asn Ser Ile Leu Leu Val Thr Lys 
1               5                   10      


<210>  25
<211>  480
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid sequence encoding a fusion protein with 
       ETEC antigens

<400>  25
atggaaatgg ctagcgcagt agaggatttt ttcattgttc cagtttctgg agatcctgca       60

attgatcttt tgcaagctga tggcaatgct ctgccatcag ctgtaaagtt agcttattct      120

cccgcatcaa aaactaatac tttggtgggt gttttgactc ttgtacatac aaacgatgca      180

actaaaaaaa atgtactagt taagcttgta acaccacagc ttacagatgt tctgaatcca      240

accctgcaaa ttcctgtttc tgtgcaggta acggtctacc ctgtttctac aacagccaaa      300

gaatttgaag ctgctgcttt gggatattct gcatccggtg taaatggctt ggtgtcaatt      360

gtgcttactg taattagcgc tgcacctaaa actgccggta ccgccccaac tgcaggaaac      420

tattcaggag tagtatctct tgtaatgact ttgggagcct gataacggcc gcactcgagc      480


<210>  26
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary fusion protein with ETEC antigens

<400>  26

Met Glu Met Ala Ser Ala Val Glu Asp Phe Phe Ile Val Pro Val Ser 
1               5                   10                  15      


Gly Asp Pro Ala Ile Asp Leu Leu Gln Ala Asp Gly Asn Ala Leu Pro 
            20                  25                  30          


Ser Ala Val Lys Leu Ala Tyr Ser Pro Ala Ser Lys Thr Asn Thr Leu 
        35                  40                  45              


Val Gly Val Leu Thr Leu Val His Thr Asn Asp Ala Thr Lys Lys Asn 
    50                  55                  60                  


Val Leu Val Lys Leu Val Thr Pro Gln Leu Thr Asp Val Leu Asn Pro 
65                  70                  75                  80  


Thr Leu Gln Ile Pro Val Ser Val Gln Val Thr Val Tyr Pro Val Ser 
                85                  90                  95      


Thr Thr Ala Lys Glu Phe Glu Ala Ala Ala Leu Gly Tyr Ser Ala Ser 
            100                 105                 110         


Gly Val Asn Gly Leu Val Ser Ile Val Leu Thr Val Ile Ser Ala Ala 
        115                 120                 125             


Pro Lys Thr Ala Gly Thr Ala Pro Thr Ala Gly Asn Tyr Ser Gly Val 
    130                 135                 140                 


Val Ser Leu Val Met Thr Leu Gly Ala 
145                 150             


<210>  27
<211>  1278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid sequence encoding a fusion protein with 
       ETEC toxoid antigen

<400>  27
atggaaatgg ctagcatgaa tagtagcaat tactgctgtg aattgtgttg tagccctgct       60

tgtaccgggt gctatgggcc ggggcccaat ggcgacaaat tataccgtgc tgactctaga      120

cccccagatg aaataaaacg ttccggaggt cttatgccca gagggcataa tgagtacttc      180

gatagaggaa ctcaaatgaa tattaatctt tatgatcacg cgagaggaac acaaaccggc      240

tttgtcagat atgatgacgg atatgtttcc acttctctta gtttgagaag tgctcactta      300

gcaggacagt ctatattatc aggatattcc acttactata tatatgttat agcgacagca      360

ccaaatatgt ttaatgttaa tgatgtatta ggcgtataca gccctcaccc atatgaacag      420

gaggtttctg cgttaggtgg aataccatat tctcagatat atggatggta tcgtgttaat      480

tttggtgtga ttgatgaacg attacatcgt aacagggaat atagagaccg gtattacaga      540

aatctgaata tagctccggc agaggatggt tacagattag caggtttccc accggatcac      600

caagcttgga gagaagaacc ctggattcat catgcaccac aaggttgtgg aaattcatca      660

ggagggccgg tcgacatgaa tagtagcaat tactgctgtg aattgtgttg tagccctgct      720

tgtaccgggt gctatacaat tacaggtgat acttgtaatg aggagaccca gaatctgagc      780

acaatatatg ccaggaaata tcaatcaaaa gttaagaggc agatattttc agactatcag      840

tcagaggttg acatatataa cagaattcgg aatgaattag ggccggggcc cgctccccag      900

tctattacag aactatgttc ggaatatcgc aacacacaaa tatatacgat aaatgacaag      960

atactatcat atacggaatc gatggcaggc aaaagagaaa tggttatcat tacatttaag     1020

agcggcgcaa catttcaggt cgaagtcccg ggcagtcaac atatagactc ccaaaaaaaa     1080

gccattgaaa ggatgaagga cacattaaga atcacatatc tgaccgagac caaaattgat     1140

aaattatgtg tatggaataa taaaaccccc aattcaattg cggcaatcag tgatccccgg     1200

gtaccgagct cgatgaatag tagcaattac tgctgtgaat tgtgttgtag ccctgcttgt     1260

accgggtgct attaataa                                                   1278


<210>  28
<211>  424
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary fusion protein with ETEC toxoid antigen

<400>  28

Met Glu Met Ala Ser Met Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys 
1               5                   10                  15      


Cys Ser Pro Ala Cys Thr Gly Cys Tyr Gly Pro Gly Pro Asn Gly Asp 
            20                  25                  30          


Lys Leu Tyr Arg Ala Asp Ser Arg Pro Pro Asp Glu Ile Lys Arg Ser 
        35                  40                  45              


Gly Gly Leu Met Pro Arg Gly His Asn Glu Tyr Phe Asp Arg Gly Thr 
    50                  55                  60                  


Gln Met Asn Ile Asn Leu Tyr Asp His Ala Arg Gly Thr Gln Thr Gly 
65                  70                  75                  80  


Phe Val Arg Tyr Asp Asp Gly Tyr Val Ser Thr Ser Leu Ser Leu Arg 
                85                  90                  95      


Ser Ala His Leu Ala Gly Gln Ser Ile Leu Ser Gly Tyr Ser Thr Tyr 
            100                 105                 110         


Tyr Ile Tyr Val Ile Ala Thr Ala Pro Asn Met Phe Asn Val Asn Asp 
        115                 120                 125             


Val Leu Gly Val Tyr Ser Pro His Pro Tyr Glu Gln Glu Val Ser Ala 
    130                 135                 140                 


Leu Gly Gly Ile Pro Tyr Ser Gln Ile Tyr Gly Trp Tyr Arg Val Asn 
145                 150                 155                 160 


Phe Gly Val Ile Asp Glu Arg Leu His Arg Asn Arg Glu Tyr Arg Asp 
                165                 170                 175     


Arg Tyr Tyr Arg Asn Leu Asn Ile Ala Pro Ala Glu Asp Gly Tyr Arg 
            180                 185                 190         


Leu Ala Gly Phe Pro Pro Asp His Gln Ala Trp Arg Glu Glu Pro Trp 
        195                 200                 205             


Ile His His Ala Pro Gln Gly Cys Gly Asn Ser Ser Gly Gly Pro Val 
    210                 215                 220                 


Asp Met Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Ser Pro Ala 
225                 230                 235                 240 


Cys Thr Gly Cys Tyr Thr Ile Thr Gly Asp Thr Cys Asn Glu Glu Thr 
                245                 250                 255     


Gln Asn Leu Ser Thr Ile Tyr Ala Arg Lys Tyr Gln Ser Lys Val Lys 
            260                 265                 270         


Arg Gln Ile Phe Ser Asp Tyr Gln Ser Glu Val Asp Ile Tyr Asn Arg 
        275                 280                 285             


Ile Arg Asn Glu Leu Gly Pro Gly Pro Ala Pro Gln Ser Ile Thr Glu 
    290                 295                 300                 


Leu Cys Ser Glu Tyr Arg Asn Thr Gln Ile Tyr Thr Ile Asn Asp Lys 
305                 310                 315                 320 


Ile Leu Ser Tyr Thr Glu Ser Met Ala Gly Lys Arg Glu Met Val Ile 
                325                 330                 335     


Ile Thr Phe Lys Ser Gly Ala Thr Phe Gln Val Glu Val Pro Gly Ser 
            340                 345                 350         


Gln His Ile Asp Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr 
        355                 360                 365             


Leu Arg Ile Thr Tyr Leu Thr Glu Thr Lys Ile Asp Lys Leu Cys Val 
    370                 375                 380                 


Trp Asn Asn Lys Thr Pro Asn Ser Ile Ala Ala Ile Ser Asp Pro Arg 
385                 390                 395                 400 


Val Pro Ser Ser Met Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys 
                405                 410                 415     


Ser Pro Ala Cys Thr Gly Cys Tyr 
            420                 


<210>  29
<211>  1014
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleic acid sequence encoding a fusion protein with 
       shigella epitopes

<400>  29
atgggcaata taacaactct gactaatagt atttccacct catcattcag cccaaacaat       60

accaacggtt catcaaccga aacagttaat tctgatataa aaacaacgac cagttctcat      120

cctgtaagtt cccttactat gctcaacgac acccttcata atatcagaac aacaaatcag      180

gcattaaaga aagatctctc acaaaaaacg ttgactaaaa catcgctaga agaaatagca      240

ttacattcat ctcagattag catggatgta aacaaatccg ctcaactatt ggatattctt      300

tccaagaaag aatatccaat taataaagac gcaagagaat tattacattc agcccaacta      360

gttggaaaaa ataatgaaga atctatgata tctcacagag aactgtggga taaaattgca      420

aagtcaatca ataatattaa tgaacagtat ctgaaagtat atgaacatgc cgttagttca      480

tatactcaat ctgctgaaca gctatcaacc cagcagaaaa gtcttgccgg ctggatctct      540

cccggaggta acgacggaaa ctccgtgaaa ttacaagtca agtctcttaa aagtgatagt      600

gatgggggga atggaggtga taaagataaa ccgctatatc cagcaaataa tactgttctg      660

cctgactatc atggacaaga ctctgttcgt gtagaattag gtggaacaga acgtaaaccg      720

aacgcttgta aagacgagta tgttgtcaat ataaacatga ccccaataga caatatgctt      780

ccggagtatc ggggagagga tggtgtcaga gtgggtgagg ttgtgctaga taatgcaaaa      840

tatcaggcat ggaatgccgg agagtataca aaatataatg atgacgatac caatcttcaa      900

accttagttc aaaaatacag taatgccaat agtatttttg ataatttagt aaaggttttg      960

agtagtacaa taagttcatg tacagataca gataaacttt ttctccattt ctga           1014


<210>  30
<211>  337
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary fusion protein with shigella epitopes

<400>  30

Met Gly Asn Ile Thr Thr Leu Thr Asn Ser Ile Ser Thr Ser Ser Phe 
1               5                   10                  15      


Ser Pro Asn Asn Thr Asn Gly Ser Ser Thr Glu Thr Val Asn Ser Asp 
            20                  25                  30          


Ile Lys Thr Thr Thr Ser Ser His Pro Val Ser Ser Leu Thr Met Leu 
        35                  40                  45              


Asn Asp Thr Leu His Asn Ile Arg Thr Thr Asn Gln Ala Leu Lys Lys 
    50                  55                  60                  


Asp Leu Ser Gln Lys Thr Leu Thr Lys Thr Ser Leu Glu Glu Ile Ala 
65                  70                  75                  80  


Leu His Ser Ser Gln Ile Ser Met Asp Val Asn Lys Ser Ala Gln Leu 
                85                  90                  95      


Leu Asp Ile Leu Ser Lys Lys Glu Tyr Pro Ile Asn Lys Asp Ala Arg 
            100                 105                 110         


Glu Leu Leu His Ser Ala Gln Leu Val Gly Lys Asn Asn Glu Glu Ser 
        115                 120                 125             


Met Ile Ser His Arg Glu Leu Trp Asp Lys Ile Ala Lys Ser Ile Asn 
    130                 135                 140                 


Asn Ile Asn Glu Gln Tyr Leu Lys Val Tyr Glu His Ala Val Ser Ser 
145                 150                 155                 160 


Tyr Thr Gln Ser Ala Glu Gln Leu Ser Thr Gln Gln Lys Ser Leu Ala 
                165                 170                 175     


Gly Trp Ile Ser Pro Gly Gly Asn Asp Gly Asn Ser Val Lys Leu Gln 
            180                 185                 190         


Val Lys Ser Leu Lys Ser Asp Ser Asp Gly Gly Asn Gly Gly Asp Lys 
        195                 200                 205             


Asp Lys Pro Leu Tyr Pro Ala Asn Asn Thr Val Leu Pro Asp Tyr His 
    210                 215                 220                 


Gly Gln Asp Ser Val Arg Val Glu Leu Gly Gly Thr Glu Arg Lys Pro 
225                 230                 235                 240 


Asn Ala Cys Lys Asp Glu Tyr Val Val Asn Ile Asn Met Thr Pro Ile 
                245                 250                 255     


Asp Asn Met Leu Pro Glu Tyr Arg Gly Glu Asp Gly Val Arg Val Gly 
            260                 265                 270         


Glu Val Val Leu Asp Asn Ala Lys Tyr Gln Ala Trp Asn Ala Gly Glu 
        275                 280                 285             


Tyr Thr Lys Tyr Asn Asp Asp Asp Thr Asn Leu Gln Thr Leu Val Gln 
    290                 295                 300                 


Lys Tyr Ser Asn Ala Asn Ser Ile Phe Asp Asn Leu Val Lys Val Leu 
305                 310                 315                 320 


Ser Ser Thr Ile Ser Ser Cys Thr Asp Thr Asp Lys Leu Phe Leu His 
                325                 330                 335     


Phe 
    


<210>  31
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary LPS O-antigen mimic epitope

<400>  31

Cys Phe Phe Pro Asn Leu Ser Tyr Cys 
1               5                   


<210>  32
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary LPS O-antigen mimic epitope

<400>  32

Ser Met Cys Met His Gly Gly Ala Tyr Cys Phe Pro 
1               5                   10          


<210>  33
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary primer sequence

<400>  33
cccatgggca tgacattact cgaagtaatc attg                                   34


<210>  34
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary primer sequence

<400>  34
tcacggccgt taactgttac caaaagctac tgtg                                   34


<210>  35
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary primer sequence

<400>  35
ccgccatgga tgcaattatt aaaacagc                                          28


<210>  36
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary primer sequence

<400>  36
tcacggccgt taactgttac caaaagctac tg                                     32


<210>  37
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary primer sequence

<400>  37
ctagctagca tggcaattaa tgtaaacacg                                        30


<210>  38
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary primer sequence

<400>  38
tcacggccgt tattatccca ataagctcag agct                                   34


<210>  39
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary primer sequence

<400>  39
ctagctagca tggcggtgaa tgtaaacacc                                        30


<210>  40
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary primer sequence

<400>  40
tcacggccgc tagcctagaa gagccagtgc                                        30


<210>  41
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary primer sequence

<400>  41
ctagctagca tggcagtgaa tgtaaatac                                         29


<210>  42
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary primer sequence

<400>  42
tcacggccgt tattaaccca acaggctgag tg                                     32


<210>  43
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary primer sequence

<400>  43
cggaattccg atggcaacgg gtgacactga gtt                                    33


<210>  44
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary primer sequence

<400>  44
tcacggccgt tattaggtat cccaagttat acc                                    33


<210>  45
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary primer sequence

<400>  45
ccgccatgga tgccaaaact caatcg                                            26


<210>  46
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary primer sequence

<400>  46
tcacggccgg ttatcgacag gaaaggt                                           27


