<110>    CJ CheilJedang Corporation

<120>    Novel cell division membrane protein variant and a method for
         producing L-lysine using the same

<130>    OPP20212331KR

<150>    KR 10-2021-0047417
<151>    2021-04-12

<160>    10

<170>    KoPatentIn 3.0

<210>    1
<211>    441
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    (I307V)_cell division membrane protein


<400>    1
Met Asn Thr Leu Glu Arg Leu Lys Leu Arg Arg Thr Glu Met Trp Leu
  1               5                  10                  15 

Leu Ile Leu Ala Thr Leu Val Val Ser Ile Met Phe Ile Ser Leu Glu
             20                  25                  30 

Leu Ala Met Gly Asn Glu Leu Gly Thr His Ile Leu Met Leu Met Gly
         35                  40                  45 

Gly Tyr Ile Gly Ile Phe Ile Val Ala His Leu Ala Met Ala Trp Val
     50                  55                  60 

Ala Pro Phe Ala Asp Gln Ile Met Leu Pro Val Val Ala Val Leu Asn
 65                  70                  75                  80 

Gly Ile Gly Leu Val Met Ile Tyr Arg Leu Asp Glu Ala Thr Gly Tyr
                 85                  90                  95 

Ser Thr Val Asn Ser Gln Leu Met Trp Thr Val Val Gly Val Thr Leu
            100                 105                 110 

Met Val Ala Val Leu Leu Leu Leu Arg Asp Tyr Lys Ser Leu Ser Arg
        115                 120                 125 

Tyr Ser Tyr Leu Leu Gly Val Val Gly Ile Val Leu Leu Ala Leu Pro
    130                 135                 140 

Leu Val Trp Pro Gln Pro Gly Gly Val Glu Ala Arg Ile Trp Ile Trp
145                 150                 155                 160 

Leu Gly Pro Phe Ser Ile Gln Pro Gly Glu Phe Ser Lys Ile Leu Leu
                165                 170                 175 

Leu Leu Phe Phe Ala Gln Leu Leu Ala Thr Lys Arg Ala Leu Phe Thr
            180                 185                 190 

Val Ala Gly Tyr Arg Phe Leu Gly Met Asp Phe Pro Arg Leu Arg Asp
        195                 200                 205 

Leu Ala Pro Ile Leu Val Val Trp Ala Leu Ala Ile Leu Ile Met Ala
    210                 215                 220 

Gly Ala Asn Asp Phe Gly Pro Ala Leu Leu Leu Phe Thr Thr Val Leu
225                 230                 235                 240 

Ala Met Val Tyr Leu Ala Thr Gly Arg Gly Ser Trp Leu Leu Ile Gly
                245                 250                 255 

Ala Val Leu Val Ala Val Gly Ala Phe Ala Val Tyr Gln Val Ser Ser
            260                 265                 270 

Lys Ile Gln Glu Arg Val Gln Asn Phe Val Asp Pro Val Ala His Tyr
        275                 280                 285 

Asp Thr Thr Gly Tyr Gln Leu Ser Gln Ser Leu Phe Gly Met Ser Trp
    290                 295                 300 

Gly Gly Val Thr Gly Thr Gly Ile Gly Gln Gly Tyr Pro Asn Met Ile
305                 310                 315                 320 

Pro Val Val His Ser Asp Phe Ile Leu Ala Ala Ile Gly Glu Glu Leu
                325                 330                 335 

Gly Leu Ile Gly Leu Ala Ala Ile Ile Val Leu Phe Gly Val Phe Val
            340                 345                 350 

Thr Arg Gly Met Arg Thr Ala Thr Leu Ala Arg Asp Ser Tyr Gly Lys
        355                 360                 365 

Leu Val Ala Ser Gly Leu Ser Met Thr Ile Met Ile Gln Ile Phe Val
    370                 375                 380 

Val Val Ala Gly Ile Ser Ser Leu Met Pro Met Thr Gly Leu Thr Thr
385                 390                 395                 400 

Pro Phe Met Ser Gln Gly Gly Ser Ser Leu Met Ala Asn Tyr Ile Leu
                405                 410                 415 

Met Ala Ile Ile Leu Arg Ile Ser Asp Ser Ala Arg Arg Pro Val Met
            420                 425                 430 

Ser Lys Gln Ala Ser Glu Val Ala Ala
        435                 440    


<210>    2
<211>    1326
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    (I307V)_NCgl0043


<400>    2
atgaacacgc ttgaacgatt aaagcttcgt cgcacggaaa tgtggctgct gatacttgcc         60

acactcgttg tgtcgatcat gttcatcagc ctcgagctgg ccatgggcaa tgagttgggt        120

acccatattt tgatgctgat gggcggatat atcggtatct tcatcgtcgc gcacctagcc        180

atggcatggg tggcgccgtt tgctgatcaa atcatgctgc ctgtggtggc ggtgctcaat        240

ggcattggtt tggtgatgat ttatcgcctt gatgaggcca cgggctacag cacggtcaat        300

agccaattga tgtggacggt tgttggcgtc acgctgatgg tggctgtgtt gttgctgttg        360

cgtgattaca agtcgctttc gcgttattcc tacctcctcg gtgtggtggg catcgtgctg        420

ctggcgctgc ctctcgtgtg gccgcagcca ggcggcgtgg aagcccgcat ctggatttgg        480

cttggacctt tctccatcca gccaggtgag ttctccaaga ttttgctgct gctgttcttt        540

gctcagctgc tagccaccaa gcgtgctttg tttactgttg cgggctaccg tttcctcggc        600

atggatttcc ctcgtttgcg tgacctcgcg ccgattcttg tggtgtgggc gttggctatt        660

ttgatcatgg ctggcgccaa cgacttcggt cctgcactgc tgcttttcac taccgttttg        720

gccatggtgt acctggctac cggccgtggt tcctggctgt tgattggtgc tgtgttggtg        780

gctgtcggcg cgttcgcggt gtaccaagtt tcaagcaaga ttcaggaacg cgtgcaaaac        840

ttcgtggatc ctgtggccca ctatgacacc accggttacc agctgtccca gtccttgttt        900

ggcatgagtt ggggcggagt caccggcacc ggcattggtc agggttaccc caacatgatc        960

cctgtcgtgc actcggactt cattctcgca gccattggtg aggagcttgg tctgattggc       1020

ctggcggcca tcatcgtgct gtttggtgtg tttgtcaccc gcggtatgcg caccgctacc       1080

ctggctcgtg acagctacgg aaagctcgtg gcatctggtc tgtcgatgac catcatgatc       1140

cagattttcg tcgtcgtggc aggtatttct tcactgatgc ccatgacagg tttgaccact       1200

ccgtttatgt cccagggtgg ttcatccctg atggctaact acattctgat ggccatcatc       1260

ttgcgtattt ctgacagtgc ccgccgacct gtcatgtcca agcaagcatc ggaggtggct       1320

gcgtga                                                                  1326


<210>    3
<211>    441
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    (WT)_cell division membrane protein


<400>    3
Met Asn Thr Leu Glu Arg Leu Lys Leu Arg Arg Thr Glu Met Trp Leu
  1               5                  10                  15 

Leu Ile Leu Ala Thr Leu Val Val Ser Ile Met Phe Ile Ser Leu Glu
             20                  25                  30 

Leu Ala Met Gly Asn Glu Leu Gly Thr His Ile Leu Met Leu Met Gly
         35                  40                  45 

Gly Tyr Ile Gly Ile Phe Ile Val Ala His Leu Ala Met Ala Trp Val
     50                  55                  60 

Ala Pro Phe Ala Asp Gln Ile Met Leu Pro Val Val Ala Val Leu Asn
 65                  70                  75                  80 

Gly Ile Gly Leu Val Met Ile Tyr Arg Leu Asp Glu Ala Thr Gly Tyr
                 85                  90                  95 

Ser Thr Val Asn Ser Gln Leu Met Trp Thr Val Val Gly Val Thr Leu
            100                 105                 110 

Met Val Ala Val Leu Leu Leu Leu Arg Asp Tyr Lys Ser Leu Ser Arg
        115                 120                 125 

Tyr Ser Tyr Leu Leu Gly Val Val Gly Ile Val Leu Leu Ala Leu Pro
    130                 135                 140 

Leu Val Trp Pro Gln Pro Gly Gly Val Glu Ala Arg Ile Trp Ile Trp
145                 150                 155                 160 

Leu Gly Pro Phe Ser Ile Gln Pro Gly Glu Phe Ser Lys Ile Leu Leu
                165                 170                 175 

Leu Leu Phe Phe Ala Gln Leu Leu Ala Thr Lys Arg Ala Leu Phe Thr
            180                 185                 190 

Val Ala Gly Tyr Arg Phe Leu Gly Met Asp Phe Pro Arg Leu Arg Asp
        195                 200                 205 

Leu Ala Pro Ile Leu Val Val Trp Ala Leu Ala Ile Leu Ile Met Ala
    210                 215                 220 

Gly Ala Asn Asp Phe Gly Pro Ala Leu Leu Leu Phe Thr Thr Val Leu
225                 230                 235                 240 

Ala Met Val Tyr Leu Ala Thr Gly Arg Gly Ser Trp Leu Leu Ile Gly
                245                 250                 255 

Ala Val Leu Val Ala Val Gly Ala Phe Ala Val Tyr Gln Val Ser Ser
            260                 265                 270 

Lys Ile Gln Glu Arg Val Gln Asn Phe Val Asp Pro Val Ala His Tyr
        275                 280                 285 

Asp Thr Thr Gly Tyr Gln Leu Ser Gln Ser Leu Phe Gly Met Ser Trp
    290                 295                 300 

Gly Gly Ile Thr Gly Thr Gly Ile Gly Gln Gly Tyr Pro Asn Met Ile
305                 310                 315                 320 

Pro Val Val His Ser Asp Phe Ile Leu Ala Ala Ile Gly Glu Glu Leu
                325                 330                 335 

Gly Leu Ile Gly Leu Ala Ala Ile Ile Val Leu Phe Gly Val Phe Val
            340                 345                 350 

Thr Arg Gly Met Arg Thr Ala Thr Leu Ala Arg Asp Ser Tyr Gly Lys
        355                 360                 365 

Leu Val Ala Ser Gly Leu Ser Met Thr Ile Met Ile Gln Ile Phe Val
    370                 375                 380 

Val Val Ala Gly Ile Ser Ser Leu Met Pro Met Thr Gly Leu Thr Thr
385                 390                 395                 400 

Pro Phe Met Ser Gln Gly Gly Ser Ser Leu Met Ala Asn Tyr Ile Leu
                405                 410                 415 

Met Ala Ile Ile Leu Arg Ile Ser Asp Ser Ala Arg Arg Pro Val Met
            420                 425                 430 

Ser Lys Gln Ala Ser Glu Val Ala Ala
        435                 440    


<210>    4
<211>    1326
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    (WT)_NCgl0043


<400>    4
atgaacacgc ttgaacgatt aaagcttcgt cgcacggaaa tgtggctgct gatacttgcc         60

acactcgttg tgtcgatcat gttcatcagc ctcgagctgg ccatgggcaa tgagttgggt        120

acccatattt tgatgctgat gggcggatat atcggtatct tcatcgtcgc gcacctagcc        180

atggcatggg tggcgccgtt tgctgatcaa atcatgctgc ctgtggtggc ggtgctcaat        240

ggcattggtt tggtgatgat ttatcgcctt gatgaggcca cgggctacag cacggtcaat        300

agccaattga tgtggacggt tgttggcgtc acgctgatgg tggctgtgtt gttgctgttg        360

cgtgattaca agtcgctttc gcgttattcc tacctcctcg gtgtggtggg catcgtgctg        420

ctggcgctgc ctctcgtgtg gccgcagcca ggcggcgtgg aagcccgcat ctggatttgg        480

cttggacctt tctccatcca gccaggtgag ttctccaaga ttttgctgct gctgttcttt        540

gctcagctgc tagccaccaa gcgtgctttg tttactgttg cgggctaccg tttcctcggc        600

atggatttcc ctcgtttgcg tgacctcgcg ccgattcttg tggtgtgggc gttggctatt        660

ttgatcatgg ctggcgccaa cgacttcggt cctgcactgc tgcttttcac taccgttttg        720

gccatggtgt acctggctac cggccgtggt tcctggctgt tgattggtgc tgtgttggtg        780

gctgtcggcg cgttcgcggt gtaccaagtt tcaagcaaga ttcaggaacg cgtgcaaaac        840

ttcgtggatc ctgtggccca ctatgacacc accggttacc agctgtccca gtccttgttt        900

ggcatgagtt ggggcggaat caccggcacc ggcattggtc agggttaccc caacatgatc        960

cctgtcgtgc actcggactt cattctcgca gccattggtg aggagcttgg tctgattggc       1020

ctggcggcca tcatcgtgct gtttggtgtg tttgtcaccc gcggtatgcg caccgctacc       1080

ctggctcgtg acagctacgg aaagctcgtg gcatctggtc tgtcgatgac catcatgatc       1140

cagattttcg tcgtcgtggc aggtatttct tcactgatgc ccatgacagg tttgaccact       1200

ccgtttatgt cccagggtgg ttcatccctg atggctaact acattctgat ggccatcatc       1260

ttgcgtattt ctgacagtgc ccgccgacct gtcatgtcca agcaagcatc ggaggtggct       1320

gcgtga                                                                  1326


<210>    5
<211>    37
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    NCgl0043_1F


<400>    5
tgaattcgag ctcggtaccc ggtgggcatc gtgctgc                                  37


<210>    6
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    NCgl0043_2R


<400>    6
atgccggtgc cggtgactcc gccccaactc atg                                      33


<210>    7
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    NCgl0043_3F


<400>    7
catgagttgg ggcggagtca ccggcaccgg cat                                      33


<210>    8
<211>    37
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    NCgl0043_4R


<400>    8
gtcgactcta gaggatcccc ggaaaccacg tgcgttc                                  37


<210>    9
<211>    17
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    NCgl0043_5F


<400>    9
cgggctacag cacggtc                                                        17


<210>    10
<211>    17
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    NCgl0043_6R


<400>    10
gcgagtactt ggccacc                                                        17
