                               SEQUENCE LISTING

<110> GANNA BIO, INC.
      CHILDREN'S MEDICAL CENTER CORPORATION
      BETH ISRAEL DEACONESS MEDICAL CENTER, INC.
      THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR 
      UNIVERSITY
 
<120> GLYCAN MODIFIED NUCLEIC ACIDS, METHODS OF PREPARATION, AND 
      THERAPEUTIC USES

<130> 772233.202320

<150> 63/189,492
<151> 2021-05-17

<150> 63/188,930
<151> 2021-05-14

<150> 63/179,065
<151> 2021-04-23

<160> 9     

<170> PatentIn version 3.5

<210> 1
<211> 100
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: 
      Synthetic oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: 
      Synthetic oligonucleotide


<220>
<221> modified_base
<222> (60)..(60)
<223> i5OctdU

<400> 1
ggctggtccg agtgcagtgg tgtttacaac taattgatca caaccagtta cagatttctu       60

tgttccttct ccactcccac tgcttcactt gactagcctt                            100


<210> 2
<211> 80
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: 
      Synthetic oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: 
      Synthetic oligonucleotide


<220>
<221> modified_base
<222> (35)..(35)
<223> i5OctdU

<400> 2
aguuggtccg aguguugugg guuauuguua aguuuauuua acauugucuc cccccacaac       60

cgcgcuugac uagcuugcug                                                   80


<210> 3
<211> 23
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: 
      Synthetic oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> 2-OMe Ribose nucleotide

<220>
<221> misc_feature
<222> (1)..(3)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (2)..(2)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (3)..(3)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (4)..(4)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (5)..(5)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (6)..(6)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (7)..(7)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (8)..(8)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (9)..(9)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (10)..(10)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (11)..(11)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (12)..(12)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (13)..(13)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (14)..(14)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (15)..(15)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (16)..(16)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (17)..(17)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (18)..(18)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (19)..(19)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (20)..(20)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (21)..(21)
<223> 2-OMe Ribose nucleotide

<220>
<221> misc_feature
<222> (21)..(23)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (22)..(22)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (23)..(23)
<223> 2-OMe Ribose nucleotide

<400> 3
uuucgaauca auccaacagu agc                                               23


<210> 4
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: 
      Synthetic oligonucleotide


<220>
<221> misc_binding
<222> (1)..(1)
<223> 5' Cy5

<220>
<221> modified_base
<222> (1)..(1)
<223> 2-Fluororibose nucleotide

<220>
<221> misc_feature
<222> (1)..(3)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (2)..(2)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (3)..(3)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (4)..(4)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (5)..(5)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (6)..(6)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (7)..(7)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (8)..(8)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (9)..(9)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (10)..(10)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (11)..(11)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (12)..(12)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (13)..(13)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (14)..(14)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (15)..(15)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (16)..(16)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (17)..(17)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (18)..(18)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (19)..(19)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (20)..(20)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (21)..(21)
<223> 2-Fluororibose nucleotide

<220>
<221> misc_binding
<222> (21)..(21)
<223> 3' DBCO

<400> 4
uacuguugga uugauucgaa a                                                 21


<210> 5
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: 
      Synthetic oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> 2-Fluororibose nucleotide

<220>
<221> misc_feature
<222> (1)..(3)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (2)..(2)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (3)..(3)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (4)..(4)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (5)..(5)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (6)..(6)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (7)..(7)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (8)..(8)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (9)..(9)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (10)..(10)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (11)..(11)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (12)..(12)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (13)..(13)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (14)..(14)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (15)..(15)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (16)..(16)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (17)..(17)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (18)..(18)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (19)..(19)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (20)..(20)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (21)..(21)
<223> 2-Fluororibose nucleotide

<220>
<221> misc_binding
<222> (21)..(21)
<223> 3' DBCO

<400> 5
uacuguugga uugauucgaa a                                                 21


<210> 6
<211> 22
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: 
      Synthetic oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> 2-Fluororibose nucleotide

<220>
<221> misc_feature
<222> (1)..(2)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (2)..(2)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (3)..(3)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (4)..(4)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (5)..(5)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (6)..(6)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (7)..(7)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (8)..(8)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (9)..(9)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (10)..(10)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (11)..(11)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (12)..(12)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (13)..(13)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (14)..(14)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (15)..(15)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (16)..(16)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (17)..(17)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (18)..(18)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (19)..(19)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (20)..(20)
<223> 2-OMe Ribose nucleotide

<220>
<221> misc_feature
<222> (20)..(22)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (21)..(21)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (22)..(22)
<223> 2-OMe Ribose nucleotide

<400> 6
uucgaaucaa uccaacagua gc                                                22


<210> 7
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: 
      Synthetic oligonucleotide


<220>
<221> misc_binding
<222> (1)..(1)
<223> 5' (Cy5Lumi-Mal)(SHC6)

<220>
<221> modified_base
<222> (1)..(1)
<223> 2-Fluororibose nucleotide

<220>
<221> misc_feature
<222> (1)..(3)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (2)..(2)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (3)..(3)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (4)..(4)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (5)..(5)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (6)..(6)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (7)..(7)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (8)..(8)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (9)..(9)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (10)..(10)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (11)..(11)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (12)..(12)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (13)..(13)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (14)..(14)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (15)..(15)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (16)..(16)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (17)..(17)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (18)..(18)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (19)..(19)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (20)..(20)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (21)..(21)
<223> 2-Fluororibose nucleotide

<220>
<221> misc_binding
<222> (21)..(21)
<223> 3' (NHC6)(DBCO-C6NHS)

<400> 7
uacuguugga uugauucgaa a                                                 21


<210> 8
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: 
      Synthetic oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> 2-Fluororibose nucleotide

<220>
<221> misc_feature
<222> (1)..(3)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (2)..(2)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (3)..(3)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (4)..(4)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (5)..(5)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (6)..(6)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (7)..(7)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (8)..(8)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (9)..(9)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (10)..(10)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (11)..(11)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (12)..(12)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (13)..(13)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (14)..(14)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (15)..(15)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (16)..(16)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (17)..(17)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (18)..(18)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (19)..(19)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (20)..(20)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (21)..(21)
<223> 2-Fluororibose nucleotide

<220>
<221> misc_binding
<222> (21)..(21)
<223> 3' (NHC6)(DBCO-C6NHS)

<400> 8
uacuguugga uugauucgaa a                                                 21


<210> 9
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: 
      Synthetic oligonucleotide


<220>
<221> misc_binding
<222> (1)..(1)
<223> 5' (Cy5Lumi-Mal)(SHC6)

<220>
<221> modified_base
<222> (1)..(1)
<223> 2-Fluororibose nucleotide

<220>
<221> misc_feature
<222> (1)..(3)
<223> Phosphorothioate linkage

<220>
<221> modified_base
<222> (2)..(2)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (3)..(3)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (4)..(4)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (5)..(5)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (6)..(6)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (7)..(7)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (8)..(8)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (9)..(9)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (10)..(10)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (11)..(11)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (12)..(12)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (13)..(13)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (14)..(14)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (15)..(15)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (16)..(16)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (17)..(17)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (18)..(18)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (19)..(19)
<223> 2-Fluororibose nucleotide

<220>
<221> modified_base
<222> (20)..(20)
<223> 2-OMe Ribose nucleotide

<220>
<221> modified_base
<222> (21)..(21)
<223> 2-Fluororibose nucleotide

<400> 9
uacuguugga uugauucgaa a                                                 21


