SEQUENCE LISTING

<110> Cellectis S.A.

<120> NEW ANTI-MUC1 CARS AND GENE EDITED IMMUNE CELLS FOR SOLID TUMORS CANCER IMMUNOTHERAPY

<130> P345232PC00

<150> US63/182,330
<151> 2021-04-30

<150> PA202170361
<151> 2021-07-06

<160> 239

<170> PatentIn version 3.5


<210>  1

<211>  20
<212>  PRT
<213>  homo sapiens

<220>
<223>  MUC1 hypoglycosylated epitope region

<400>  1
His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr 
1               5                   10                  15      


Ala Pro Pro Ala 
            20  


<210>  2

<211>  21
<212>  PRT
<213>  homo sapiens

<220>
<223>  CD8&#945; signal peptide

<400>  2
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  3

<211>  40
<212>  PRT
<213>  homo sapiens

<220>
<223>  R2 suicide switch

<400>  3
Ser Asp Pro Gly Ser Gly Gly Gly Gly Ser Cys Pro Tyr Ser Asn Pro 
1               5                   10                  15      


Ser Leu Cys Ser Gly Gly Gly Gly Ser Cys Pro Tyr Ser Asn Pro Ser 
            20                  25                  30          


Leu Cys Ser Gly Gly Gly Gly Ser 
        35                  40  


<210>  4

<211>  45
<212>  PRT
<213>  homo sapiens

<220>
<223>  CD8&#945; hinge

<400>  4
Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  5

<211>  24
<212>  PRT
<213>  homo sapiens

<220>
<223>  CD8&#945; transmembrane domain

<400>  5
Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  6

<211>  42
<212>  PRT
<213>  homo sapiens

<220>
<223>  4-1BB co-stimulatory domain

<400>  6
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  7

<211>  112
<212>  PRT
<213>  homo sapiens

<220>
<223>  CD3&#950; signalling domain

<400>  7
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  8

<211>  15
<212>  PRT
<213>  artifical

<220>
<223>  Linker

<400>  8
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  9

<211>  118
<212>  PRT
<213>  artifical

<220>
<223>  heavy chain variable region MUC1-A

<400>  9
Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Leu Ser Trp Val Lys Gln Arg Thr Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Asn His Pro Gly Ser Gly Ile Ile Tyr His Asn Glu Lys Phe 
    50                  55                  60                  


Arg Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Val Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Ser Ser Gly Thr Arg Gly Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala 
        115             


<210>  10

<211>  114
<212>  PRT
<213>  artifical

<220>
<223>  light chain variable region MUC1-A

<400>  10
Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      


Ser His Gly Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Ala 
        


<210>  11

<211>  5
<212>  PRT
<213>  artifical

<220>
<223>  CDR1VH-A

<400>  11
Asn Tyr Gly Leu Ser 
1               5   


<210>  12

<211>  17
<212>  PRT
<213>  artifical

<220>
<223>  CDR2VH-A

<400>  12
Glu Asn His Pro Gly Ser Gly Ile Ile Tyr His Asn Glu Lys Phe Arg 
1               5                   10                  15      


Gly 
    


<210>  13

<211>  9
<212>  PRT
<213>  artifical

<220>
<223>  CDR3VH-A

<400>  13
Ser Ser Gly Thr Arg Gly Phe Ala Tyr 
1               5                   


<210>  14

<211>  16
<212>  PRT
<213>  artifical

<220>
<223>  CDR1VL-A

<400>  14
Arg Ser Ser Gln Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu Glu 
1               5                   10                  15      



<210>  15

<211>  7
<212>  PRT
<213>  artifical

<220>
<223>  CDR2VL-A

<400>  15
Lys Val Ser Asn Arg Phe Ser 
1               5           


<210>  16

<211>  9
<212>  PRT
<213>  artifical

<220>
<223>  CDR3VL-A

<400>  16
Phe Gln Gly Ser His Gly Pro Trp Thr 
1               5                   


<210>  17

<211>  247
<212>  PRT
<213>  artifical

<220>
<223>  MUC1-A full Binding domain

<400>  17
Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Leu Ser Trp Val Lys Gln Arg Thr Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Asn His Pro Gly Ser Gly Ile Ile Tyr His Asn Glu Lys Phe 
    50                  55                  60                  


Arg Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Val Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Ser Ser Gly Thr Arg Gly Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu 
    130                 135                 140                 


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
145                 150                 155                 160 


Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln 
                165                 170                 175     


Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg 
            180                 185                 190         


Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
        195                 200                 205             


Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
    210                 215                 220                 


Tyr Cys Phe Gln Gly Ser His Gly Pro Trp Thr Phe Gly Gly Gly Thr 
225                 230                 235                 240 


Lys Leu Glu Ile Lys Arg Ala 
                245         


<210>  18

<211>  528
<212>  PRT
<213>  artifical

<220>
<223>  CLS MUC1-A CAR full sequence

<400>  18
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu 
            20                  25                  30          


Ala Arg Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Asn Tyr Gly Leu Ser Trp Val Lys Gln Arg Thr Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Glu Asn His Pro Gly Ser Gly Ile Ile Tyr 
65                  70                  75                  80  


His Asn Glu Lys Phe Arg Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser 
                85                  90                  95      


Ser Ser Thr Ala Tyr Val Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser 
            100                 105                 110         


Ala Val Tyr Phe Cys Ala Arg Ser Ser Gly Thr Arg Gly Phe Ala Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Leu Met Thr Gln 
145                 150                 155                 160 


Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser 
                165                 170                 175     


Cys Arg Ser Ser Gln Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu 
            180                 185                 190         


Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr 
        195                 200                 205             


Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu 
225                 230                 235                 240 


Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Gly Pro Trp Thr 
                245                 250                 255     


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Gly Ser Gly Gly 
            260                 265                 270         


Gly Gly Ser Cys Pro Tyr Ser Asn Pro Ser Leu Cys Ser Gly Gly Gly 
        275                 280                 285             


Gly Ser Cys Pro Tyr Ser Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly 
    290                 295                 300                 


Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile 
305                 310                 315                 320 


Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala 
                325                 330                 335     


Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr 
            340                 345                 350         


Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu 
        355                 360                 365             


Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile 
    370                 375                 380                 


Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp 
385                 390                 395                 400 


Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
                405                 410                 415     


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
            420                 425                 430         


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
        435                 440                 445             


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
    450                 455                 460                 


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
465                 470                 475                 480 


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
                485                 490                 495     


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
            500                 505                 510         


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        515                 520                 525             



<210>  19

<211>  117
<212>  PRT
<213>  artifical

<220>
<223>  heavy chain variable region MUC1-B

<400>  19
Glu Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Met Lys Leu Ser Cys Val Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Glu Ile Arg Leu Lys Ser Asn Asn Tyr Ala Thr His Tyr Ala Glu 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Ser 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Asn Leu Arg Ala Glu Asp Thr Gly Ile Tyr 
                85                  90                  95      


Tyr Cys Thr Phe Gly Asn Ser Phe Ala Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  20

<211>  109
<212>  PRT
<213>  artifical

<220>
<223>  light chain variable region MUC1-B

<400>  20
Asp Ile Val Val Thr Gln Glu Ser Ala Leu Thr Thr Ser Pro Gly Glu 
1               5                   10                  15      


Thr Val Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser 
            20                  25                  30          


Asn Tyr Ala Asn Trp Val Gln Glu Lys Pro Asp His Leu Phe Thr Gly 
        35                  40                  45              


Leu Ile Gly Gly Thr Asn Asn Arg Ala Pro Gly Val Pro Ala Arg Phe 
    50                  55                  60                  


Ser Gly Ser Leu Ile Gly Asp Lys Ala Ala Leu Thr Ile Thr Gly Ala 
65                  70                  75                  80  


Gln Thr Glu Asp Glu Ala Ile Tyr Phe Cys Ala Leu Trp Tyr Ser Asn 
                85                  90                  95      


His Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 


<210>  21

<211>  10
<212>  PRT
<213>  artifical

<220>
<223>  CDR1VH-B

<400>  21
Gly Phe Thr Phe Ser Asn Tyr Trp Met Asn 
1               5                   10  


<210>  22

<211>  19
<212>  PRT
<213>  artifical

<220>
<223>  CDR2VH-B

<400>  22
Glu Ile Arg Leu Lys Ser Asn Asn Tyr Ala Thr His Tyr Ala Glu Ser 
1               5                   10                  15      


Val Lys Gly 
            


<210>  23

<211>  6
<212>  PRT
<213>  artifical

<220>
<223>  CDR3VH-B

<400>  23
Gly Asn Ser Phe Ala Tyr 
1               5       


<210>  24

<211>  11
<212>  PRT
<213>  artifical

<220>
<223>  CDR1VL-B

<400>  24
Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn 
1               5                   10      


<210>  25

<211>  8
<212>  PRT
<213>  artifical

<220>
<223>  CDR2VL-B

<400>  25
Gly Gly Thr Asn Asn Arg Ala Pro 
1               5               


<210>  26

<211>  9
<212>  PRT
<213>  artifical

<220>
<223>  CDR3VL-B

<400>  26
Ala Leu Trp Tyr Ser Asn His Trp Val 
1               5                   


<210>  27

<211>  241
<212>  PRT
<213>  artifical

<220>
<223>  MUC1-B full Binding domain

<400>  27
Glu Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Met Lys Leu Ser Cys Val Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Glu Ile Arg Leu Lys Ser Asn Asn Tyr Ala Thr His Tyr Ala Glu 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Ser 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Asn Leu Arg Ala Glu Asp Thr Gly Ile Tyr 
                85                  90                  95      


Tyr Cys Thr Phe Gly Asn Ser Phe Ala Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Asp Ile Val Val Thr Gln Glu Ser Ala Leu Thr Thr 
    130                 135                 140                 


Ser Pro Gly Glu Thr Val Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala 
145                 150                 155                 160 


Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln Glu Lys Pro Asp His 
                165                 170                 175     


Leu Phe Thr Gly Leu Ile Gly Gly Thr Asn Asn Arg Ala Pro Gly Val 
            180                 185                 190         


Pro Ala Arg Phe Ser Gly Ser Leu Ile Gly Asp Lys Ala Ala Leu Thr 
        195                 200                 205             


Ile Thr Gly Ala Gln Thr Glu Asp Glu Ala Ile Tyr Phe Cys Ala Leu 
    210                 215                 220                 


Trp Tyr Ser Asn His Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val 
225                 230                 235                 240 


Leu 
    


<210>  28

<211>  522
<212>  PRT
<213>  artifical

<220>
<223>  CLS MUC1-B CAR  - Full sequence

<400>  28
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Gln Gln Ser Gly Gly Gly Leu 
            20                  25                  30          


Val Gln Pro Gly Gly Ser Met Lys Leu Ser Cys Val Ala Ser Gly Phe 
        35                  40                  45              


Thr Phe Ser Asn Tyr Trp Met Asn Trp Val Arg Gln Ser Pro Glu Lys 
    50                  55                  60                  


Gly Leu Glu Trp Val Ala Glu Ile Arg Leu Lys Ser Asn Asn Tyr Ala 
65                  70                  75                  80  


Thr His Tyr Ala Glu Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
                85                  90                  95      


Asp Ser Lys Ser Ser Val Tyr Leu Gln Met Asn Asn Leu Arg Ala Glu 
            100                 105                 110         


Asp Thr Gly Ile Tyr Tyr Cys Thr Phe Gly Asn Ser Phe Ala Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Val Thr Gln Glu 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ser Pro Gly Glu Thr Val Thr Leu Thr Cys Arg 
                165                 170                 175     


Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln 
            180                 185                 190         


Glu Lys Pro Asp His Leu Phe Thr Gly Leu Ile Gly Gly Thr Asn Asn 
        195                 200                 205             


Arg Ala Pro Gly Val Pro Ala Arg Phe Ser Gly Ser Leu Ile Gly Asp 
    210                 215                 220                 


Lys Ala Ala Leu Thr Ile Thr Gly Ala Gln Thr Glu Asp Glu Ala Ile 
225                 230                 235                 240 


Tyr Phe Cys Ala Leu Trp Tyr Ser Asn His Trp Val Phe Gly Gly Gly 
                245                 250                 255     


Thr Lys Leu Thr Val Leu Gly Ser Gly Gly Gly Gly Ser Cys Pro Tyr 
            260                 265                 270         


Ser Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Cys Pro Tyr Ser 
        275                 280                 285             


Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Thr Thr Thr Pro Ala 
    290                 295                 300                 


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
305                 310                 315                 320 


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
                325                 330                 335     


Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala 
            340                 345                 350         


Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys 
        355                 360                 365             


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
    370                 375                 380                 


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
385                 390                 395                 400 


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
                405                 410                 415     


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
            420                 425                 430         


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
        435                 440                 445             


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
    450                 455                 460                 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
465                 470                 475                 480 


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
                485                 490                 495     


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
            500                 505                 510         


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        515                 520         


<210>  29

<211>  120
<212>  PRT
<213>  artifical

<220>
<223>  heavy chain variable region MUC1-C

<400>  29
Gln Met Gln Leu Val Gln Ser Glu Ala Glu Leu Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly His 
            20                  25                  30          


Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asp Pro Val Thr Gly Gly Thr Lys Tyr Ala Gln Asn Phe 
    50                  55                  60                  


Gln Gly Trp Val Thr Met Thr Arg Asp Thr Ser Ile Arg Thr Ala Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Val Thr Gly Asp Arg Gly Gln Phe Asp Lys Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ala Ser 
        115                 120 


<210>  30

<211>  107
<212>  PRT
<213>  artifical

<220>
<223>  light chain variable region MUC1-C

<400>  30
Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Val Ala Pro Gly Lys 
1               5                   10                  15      


Thr Ala Arg Ile Thr Cys Gly Gly Asn Asn Ile Gly Ser Lys Ser Val 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Ala Leu Val Ile Tyr 
        35                  40                  45              


Tyr Gly Ser Asn Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser Gly Ser 
    50                  55                  60                  


Asn Ser Gly Asn Thr Ala Thr Leu Thr Ile Ser Arg Val Glu Ala Gly 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Gln Val Trp Asp Ser Ser Ser Asp Trp 
                85                  90                  95      


Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105         


<210>  31

<211>  8
<212>  PRT
<213>  artifical

<220>
<223>  CDR1VH-C

<400>  31
Gly Tyr Ser Phe Thr Gly His Tyr 
1               5               


<210>  32

<211>  8
<212>  PRT
<213>  artifical

<220>
<223>  CDR2VH-C

<400>  32
Ile Asp Pro Val Thr Gly Gly Thr 
1               5               


<210>  33

<211>  10
<212>  PRT
<213>  artifical

<220>
<223>  CDR3VH-C

<400>  33
Ala Arg Glu Val Thr Gly Asp Arg Gly Gln 
1               5                   10  


<210>  34

<211>  6
<212>  PRT
<213>  artifical

<220>
<223>  CDR1VL-C

<400>  34
Asn Ile Gly Ser Lys Ser 
1               5       


<210>  35

<211>  3
<212>  PRT
<213>  artifical

<220>
<223>  CDR2VL-C

<400>  35
Tyr Gly Ser 
1           


<210>  36

<211>  10
<212>  PRT
<213>  artifical

<220>
<223>  CDR3VL-C

<400>  36
Gln Val Trp Asp Ser Ser Ser Asp Trp Val 
1               5                   10  


<210>  37

<211>  242
<212>  PRT
<213>  artifical

<220>
<223>  MUC1-C full Binding domain

<400>  37
Gln Met Gln Leu Val Gln Ser Glu Ala Glu Leu Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly His 
            20                  25                  30          


Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asp Pro Val Thr Gly Gly Thr Lys Tyr Ala Gln Asn Phe 
    50                  55                  60                  


Gln Gly Trp Val Thr Met Thr Arg Asp Thr Ser Ile Arg Thr Ala Tyr 
65                  70                  75                  80  


Leu Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Val Thr Gly Asp Arg Gly Gln Phe Asp Lys Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ala Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser 
    130                 135                 140                 


Val Ser Val Ala Pro Gly Lys Thr Ala Arg Ile Thr Cys Gly Gly Asn 
145                 150                 155                 160 


Asn Ile Gly Ser Lys Ser Val His Trp Tyr Gln Gln Lys Pro Gly Gln 
                165                 170                 175     


Ala Pro Ala Leu Val Ile Tyr Tyr Gly Ser Asn Arg Pro Ser Gly Ile 
            180                 185                 190         


Pro Glu Arg Phe Ser Gly Ser Asn Ser Gly Asn Thr Ala Thr Leu Thr 
        195                 200                 205             


Ile Ser Arg Val Glu Ala Gly Asp Glu Ala Asp Tyr Tyr Cys Gln Val 
    210                 215                 220                 


Trp Asp Ser Ser Ser Asp Trp Val Phe Gly Gly Gly Thr Lys Leu Thr 
225                 230                 235                 240 


Val Leu 
        


<210>  38

<211>  523
<212>  PRT
<213>  artifical

<220>
<223>  CLS MUC1-C CAR - Full sequence

<400>  38
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Met Gln Leu Val Gln Ser Glu Ala Glu Leu 
            20                  25                  30          


Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr 
        35                  40                  45              


Ser Phe Thr Gly His Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Met Gly Trp Ile Asp Pro Val Thr Gly Gly Thr Lys 
65                  70                  75                  80  


Tyr Ala Gln Asn Phe Gln Gly Trp Val Thr Met Thr Arg Asp Thr Ser 
                85                  90                  95      


Ile Arg Thr Ala Tyr Leu Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr 
            100                 105                 110         


Ala Met Tyr Tyr Cys Ala Arg Glu Val Thr Gly Asp Arg Gly Gln Phe 
        115                 120                 125             


Asp Lys Trp Gly Gln Gly Thr Leu Val Thr Val Ala Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ser Val Leu 
145                 150                 155                 160 


Thr Gln Pro Pro Ser Val Ser Val Ala Pro Gly Lys Thr Ala Arg Ile 
                165                 170                 175     


Thr Cys Gly Gly Asn Asn Ile Gly Ser Lys Ser Val His Trp Tyr Gln 
            180                 185                 190         


Gln Lys Pro Gly Gln Ala Pro Ala Leu Val Ile Tyr Tyr Gly Ser Asn 
        195                 200                 205             


Arg Pro Ser Gly Ile Pro Glu Arg Phe Ser Gly Ser Asn Ser Gly Asn 
    210                 215                 220                 


Thr Ala Thr Leu Thr Ile Ser Arg Val Glu Ala Gly Asp Glu Ala Asp 
225                 230                 235                 240 


Tyr Tyr Cys Gln Val Trp Asp Ser Ser Ser Asp Trp Val Phe Gly Gly 
                245                 250                 255     


Gly Thr Lys Leu Thr Val Leu Gly Ser Gly Gly Gly Gly Ser Cys Pro 
            260                 265                 270         


Tyr Ser Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Cys Pro Tyr 
        275                 280                 285             


Ser Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Thr Thr Thr Pro 
    290                 295                 300                 


Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu 
305                 310                 315                 320 


Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His 
                325                 330                 335     


Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu 
            340                 345                 350         


Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr 
        355                 360                 365             


Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
    370                 375                 380                 


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
385                 390                 395                 400 


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
                405                 410                 415     


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
            420                 425                 430         


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
        435                 440                 445             


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
    450                 455                 460                 


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
465                 470                 475                 480 


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
                485                 490                 495     


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
            500                 505                 510         


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        515                 520             


<210>  39

<211>  116
<212>  PRT
<213>  artifical

<220>
<223>  heavy chain variable region MUC1-D

<400>  39
Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 
            20                  25                  30          


Ala Ile His Trp Val Lys Gln Lys Pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly His Phe Ser Pro Gly Asn Thr Asp Ile Lys Tyr Asn Asp Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Arg Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Lys Thr Ser Thr Phe Phe Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  40

<211>  113
<212>  PRT
<213>  artifical

<220>
<223>  light chain variable region MUC1-D

<400>  40
Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Thr Val Thr Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ile Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Gly Asp Gln Lys Asn Tyr Leu Thr Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Phe Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp Tyr Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
            100                 105                 110         


Lys 
    


<210>  41

<211>  9
<212>  PRT
<213>  artifical

<220>
<223>  CDR1VH-D

<400>  41
Tyr Thr Phe Thr Asp His Ala Ile His 
1               5                   


<210>  42

<211>  14
<212>  PRT
<213>  artifical

<220>
<223>  CDR2VH-D

<400>  42
Trp Ile Gly His Phe Ser Pro Gly Asn Thr Asp Ile Lys Tyr 
1               5                   10                  


<210>  43

<211>  9
<212>  PRT
<213>  artifical

<220>
<223>  CDR3VH-D

<400>  43
Lys Thr Ser Thr Phe Phe Phe Asp Tyr 
1               5                   


<210>  44

<211>  14
<212>  PRT
<213>  artifical

<220>
<223>  CDR1VL-D

<400>  44
Gln Ser Leu Leu Asn Ser Gly Asp Gln Lys Asn Tyr Leu Thr 
1               5                   10                  


<210>  45

<211>  11
<212>  PRT
<213>  artifical

<220>
<223>  CDR2VL-D

<400>  45
Leu Leu Ile Phe Trp Ala Ser Thr Arg Glu Ser 
1               5                   10      


<210>  46

<211>  8
<212>  PRT
<213>  artifical

<220>
<223>  CDR3VL-D

<400>  46
Gln Asn Asp Tyr Ser Tyr Pro Leu 
1               5               


<210>  47

<211>  244
<212>  PRT
<213>  artifical

<220>
<223>  MUC1-D full Binding domain

<400>  47
Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 
            20                  25                  30          


Ala Ile His Trp Val Lys Gln Lys Pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly His Phe Ser Pro Gly Asn Thr Asp Ile Lys Tyr Asn Asp Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Arg Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Lys Thr Ser Thr Phe Phe Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Thr Val 
    130                 135                 140                 


Thr Ala Gly Glu Lys Val Thr Met Ile Cys Lys Ser Ser Gln Ser Leu 
145                 150                 155                 160 


Leu Asn Ser Gly Asp Gln Lys Asn Tyr Leu Thr Trp Tyr Gln Gln Lys 
                165                 170                 175     


Pro Gly Gln Pro Pro Lys Leu Leu Ile Phe Trp Ala Ser Thr Arg Glu 
            180                 185                 190         


Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe 
        195                 200                 205             


Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr 
    210                 215                 220                 


Cys Gln Asn Asp Tyr Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys 
225                 230                 235                 240 


Leu Glu Leu Lys 
                


<210>  48

<211>  525
<212>  PRT
<213>  artifical

<220>
<223>  CLS MUC1-D CAR - Full sequence

<400>  48
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu 
            20                  25                  30          


Val Lys Pro Gly Ser Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Asp His Ala Ile His Trp Val Lys Gln Lys Pro Glu Gln 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly His Phe Ser Pro Gly Asn Thr Asp Ile Lys 
65                  70                  75                  80  


Tyr Asn Asp Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Arg Ser 
                85                  90                  95      


Ser Ser Thr Ala Tyr Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser 
            100                 105                 110         


Ala Val Tyr Phe Cys Lys Thr Ser Thr Phe Phe Phe Asp Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Ser Pro 
145                 150                 155                 160 


Ser Ser Leu Thr Val Thr Ala Gly Glu Lys Val Thr Met Ile Cys Lys 
                165                 170                 175     


Ser Ser Gln Ser Leu Leu Asn Ser Gly Asp Gln Lys Asn Tyr Leu Thr 
            180                 185                 190         


Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Phe Trp 
        195                 200                 205             


Ala Ser Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly 
    210                 215                 220                 


Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp 
225                 230                 235                 240 


Leu Ala Val Tyr Tyr Cys Gln Asn Asp Tyr Ser Tyr Pro Leu Thr Phe 
                245                 250                 255     


Gly Ala Gly Thr Lys Leu Glu Leu Lys Gly Ser Gly Gly Gly Gly Ser 
            260                 265                 270         


Cys Pro Tyr Ser Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Cys 
        275                 280                 285             


Pro Tyr Ser Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Thr Thr 
    290                 295                 300                 


Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln 
305                 310                 315                 320 


Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala 
                325                 330                 335     


Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala 
            340                 345                 350         


Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
        355                 360                 365             


Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
    370                 375                 380                 


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
385                 390                 395                 400 


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
                405                 410                 415     


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
            420                 425                 430         


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
        435                 440                 445             


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
    450                 455                 460                 


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
465                 470                 475                 480 


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
                485                 490                 495     


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
            500                 505                 510         


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        515                 520                 525 


<210>  49

<211>  9
<212>  PRT
<213>  artifical

<220>
<223>  Mimotope Rituximab

<400>  49
Cys Pro Tyr Ser Asn Pro Ser Leu Cys 
1               5                   


<210>  20

<211>  24
<212>  PRT
<213>  artifical

<220>
<223>  Epitope Palivizumab

<400>  20
Asn Ser Glu Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp 
1               5                   10                  15      


Gln Lys Lys Leu Met Ser Asn Asn 
            20                  


<210>  51

<211>  12
<212>  PRT
<213>  artifical

<220>
<223>  Mimotope 1 Cetuximab

<400>  51
Cys Gln Phe Asp Leu Ser Thr Arg Arg Leu Lys Cys 
1               5                   10          


<210>  52

<211>  12
<212>  PRT
<213>  artifical

<220>
<223>  Mimotope 2 Cetuximab

<400>  52
Cys Gln Tyr Asn Leu Ser Ser Arg Ala Leu Lys Cys 
1               5                   10          


<210>  53

<211>  12
<212>  PRT
<213>  artifical

<220>
<223>  Mimotope 3 Cetuximab

<400>  53
Cys Val Trp Gln Arg Trp Gln Lys Ser Tyr Val Cys 
1               5                   10          


<210>  54

<211>  12
<212>  PRT
<213>  artifical

<220>
<223>  Mimotope 4 Cetuximab

<400>  54
Cys Met Trp Asp Arg Phe Ser Arg Trp Tyr Lys Cys 
1               5                   10          


<210>  55

<211>  25
<212>  PRT
<213>  artifical

<220>
<223>  Epitope 1 Nivolumab

<400>  55
Ser Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp 
1               5                   10                  15      


Lys Leu Ala Ala Phe Pro Glu Asp Arg 
            20                  25  


<210>  56

<211>  19
<212>  PRT
<213>  artifical

<220>
<223>  Epitope 2 Nivolumab

<400>  56
Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln 
1               5                   10                  15      


Ile Lys Glu 
            


<210>  57

<211>  24
<212>  PRT
<213>  artifical

<220>
<223>  QBEND-10 Epitope

<400>  57
Glu Leu Pro Thr Gln Gly Thr Phe Ser Asn Val Ser Thr Asn Val Ser 
1               5                   10                  15      


Pro Ala Lys Pro Thr Thr Thr Ala 
            20                  


<210>  58

<211>  12
<212>  PRT
<213>  artifical

<220>
<223>  Alemtuzumab Epitope

<400>  58
Gly Gln Asn Asp Thr Ser Gln Thr Ser Ser Pro Ser 
1               5                   10          


<210>  59

<211>  299
<212>  PRT
<213>  artifical

<220>
<223>  dnTGFbetaR2

<400>  59
Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
1               5                   10                  15      


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
            20                  25                  30          


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
        35                  40                  45              


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
    50                  55                  60                  


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly 
65                  70                  75                  80  


Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
                85                  90                  95      


Asn Pro Gly Pro Met Gly Arg Gly Leu Leu Arg Gly Leu Trp Pro Leu 
            100                 105                 110         


His Ile Val Leu Trp Thr Arg Ile Ala Ser Thr Ile Pro Pro His Val 
        115                 120                 125             


Gln Lys Ser Val Asn Asn Asp Met Ile Val Thr Asp Asn Asn Gly Ala 
    130                 135                 140                 


Val Lys Phe Pro Gln Leu Cys Lys Phe Cys Asp Val Arg Phe Ser Thr 
145                 150                 155                 160 


Cys Asp Asn Gln Lys Ser Cys Met Ser Asn Cys Ser Ile Thr Ser Ile 
                165                 170                 175     


Cys Glu Lys Pro Gln Glu Val Cys Val Ala Val Trp Arg Lys Asn Asp 
            180                 185                 190         


Glu Asn Ile Thr Leu Glu Thr Val Cys His Asp Pro Lys Leu Pro Tyr 
        195                 200                 205             


His Asp Phe Ile Leu Glu Asp Ala Ala Ser Pro Lys Cys Ile Met Lys 
    210                 215                 220                 


Glu Lys Lys Lys Pro Gly Glu Thr Phe Phe Met Cys Ser Cys Ser Ser 
225                 230                 235                 240 


Asp Glu Cys Asn Asp Asn Ile Ile Phe Ser Glu Glu Tyr Asn Thr Ser 
                245                 250                 255     


Asn Pro Asp Leu Leu Leu Val Ile Phe Gln Val Thr Gly Ile Ser Leu 
            260                 265                 270         


Leu Pro Pro Leu Gly Val Ala Ile Ser Val Ile Ile Ile Phe Tyr Cys 
        275                 280                 285             


Tyr Arg Val Asn Arg Gln Gln Lys Leu Ser Ser 
    290                 295                 


<210>  60

<211>  335
<212>  PRT
<213>  artifical

<220>
<223>  HLA-E

<400>  60
His Ser Leu Lys Tyr Phe His Thr Ser Val Ser Arg Pro Gly Arg Gly 
1               5                   10                  15      


Glu Pro Arg Phe Ile Ser Val Gly Tyr Val Asp Asp Thr Gln Phe Val 
            20                  25                  30          


Arg Phe Asp Asn Asp Ala Ala Ser Pro Arg Met Val Pro Arg Ala Pro 
        35                  40                  45              


Trp Met Glu Gln Glu Gly Ser Glu Tyr Trp Asp Arg Glu Thr Arg Ser 
    50                  55                  60                  


Ala Arg Asp Thr Ala Gln Ile Phe Arg Val Asn Leu Arg Thr Leu Arg 
65                  70                  75                  80  


Gly Tyr Tyr Asn Gln Ser Glu Ala Gly Ser His Thr Leu Gln Trp Met 
                85                  90                  95      


His Gly Cys Glu Leu Gly Pro Asp Arg Arg Phe Leu Arg Gly Tyr Glu 
            100                 105                 110         


Gln Phe Ala Tyr Asp Gly Lys Asp Tyr Leu Thr Leu Asn Glu Asp Leu 
        115                 120                 125             


Arg Ser Trp Thr Ala Val Asp Thr Ala Ala Gln Ile Ser Glu Gln Lys 
    130                 135                 140                 


Ser Asn Asp Ala Ser Glu Ala Glu His Gln Arg Ala Tyr Leu Glu Asp 
145                 150                 155                 160 


Thr Cys Val Glu Trp Leu His Lys Tyr Leu Glu Lys Gly Lys Glu Thr 
                165                 170                 175     


Leu Leu His Leu Glu Pro Pro Lys Thr His Val Thr His His Pro Ile 
            180                 185                 190         


Ser Asp His Glu Ala Thr Leu Arg Cys Trp Ala Leu Gly Phe Tyr Pro 
        195                 200                 205             


Ala Glu Ile Thr Leu Thr Trp Gln Gln Asp Gly Glu Gly His Thr Gln 
    210                 215                 220                 


Asp Thr Glu Leu Val Glu Thr Arg Pro Ala Gly Asp Gly Thr Phe Gln 
225                 230                 235                 240 


Lys Trp Ala Ala Val Val Val Pro Ser Gly Glu Glu Gln Arg Tyr Thr 
                245                 250                 255     


Cys His Val Gln His Glu Gly Leu Pro Glu Pro Val Thr Leu Arg Trp 
            260                 265                 270         


Lys Pro Ala Ser Gln Pro Thr Ile Pro Ile Val Gly Ile Ile Ala Gly 
        275                 280                 285             


Leu Val Leu Leu Gly Ser Val Val Ser Gly Ala Val Val Ala Ala Val 
    290                 295                 300                 


Ile Trp Arg Lys Lys Ser Ser Gly Gly Lys Gly Gly Ser Tyr Tyr Lys 
305                 310                 315                 320 


Ala Glu Trp Ser Asp Ser Ala Gln Gly Ser Glu Ser His Ser Leu 
                325                 330                 335 


<210>  61

<211>  603
<212>  PRT
<213>  artifical

<220>
<223>  HLA-G

<400>  61
Met Trp Pro Pro Gly Ser Ala Ser Gln Pro Pro Pro Ser Pro Ala Ala 
1               5                   10                  15      


Ala Thr Gly Leu His Pro Ala Ala Arg Pro Val Ser Leu Gln Cys Arg 
            20                  25                  30          


Leu Ser Met Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr Leu Val 
        35                  40                  45              


Leu Leu Asp His Leu Ser Leu Ala Arg Asn Leu Pro Val Ala Thr Pro 
    50                  55                  60                  


Asp Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg 
65                  70                  75                  80  


Ala Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr 
                85                  90                  95      


Pro Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys 
            100                 105                 110         


Thr Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu 
        115                 120                 125             


Ser Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys 
    130                 135                 140                 


Leu Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser 
145                 150                 155                 160 


Ile Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn 
                165                 170                 175     


Ala Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn 
            180                 185                 190         


Met Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser 
        195                 200                 205             


Glu Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys 
    210                 215                 220                 


Thr Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala 
225                 230                 235                 240 


Val Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser Gly Ser Gly 
                245                 250                 255     


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
            260                 265                 270         


Pro Gly Pro Met Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu 
        275                 280                 285             


Val Phe Leu Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp 
    290                 295                 300                 


Val Tyr Val Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met 
305                 310                 315                 320 


Val Val Leu Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr 
                325                 330                 335     


Leu Asp Gln Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile 
            340                 345                 350         


Gln Val Lys Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly 
        355                 360                 365             


Gly Glu Val Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp 
    370                 375                 380                 


Gly Ile Trp Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn 
385                 390                 395                 400 


Lys Thr Phe Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr 
                405                 410                 415     


Cys Trp Trp Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys 
            420                 425                 430         


Ser Ser Arg Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala 
        435                 440                 445             


Thr Leu Ser Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr 
    450                 455                 460                 


Ser Val Glu Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser 
465                 470                 475                 480 


Leu Pro Ile Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu 
                485                 490                 495     


Asn Tyr Thr Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro 
            500                 505                 510         


Pro Lys Asn Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu 
        515                 520                 525             


Val Ser Trp Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe 
    530                 535                 540                 


Ser Leu Thr Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys 
545                 550                 555                 560 


Lys Asp Arg Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg 
                565                 570                 575     


Lys Asn Ala Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser 
            580                 585                 590         


Ser Trp Ser Glu Trp Ala Ser Val Pro Cys Ser 
        595                 600             


<210>  62

<211>  603
<212>  PRT
<213>  artifical

<220>
<223>  IL-12a-P2A- IL-12b

<400>  62
Met Trp Pro Pro Gly Ser Ala Ser Gln Pro Pro Pro Ser Pro Ala Ala 
1               5                   10                  15      


Ala Thr Gly Leu His Pro Ala Ala Arg Pro Val Ser Leu Gln Cys Arg 
            20                  25                  30          


Leu Ser Met Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr Leu Val 
        35                  40                  45              


Leu Leu Asp His Leu Ser Leu Ala Arg Asn Leu Pro Val Ala Thr Pro 
    50                  55                  60                  


Asp Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg 
65                  70                  75                  80  


Ala Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr 
                85                  90                  95      


Pro Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys 
            100                 105                 110         


Thr Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu 
        115                 120                 125             


Ser Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys 
    130                 135                 140                 


Leu Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser 
145                 150                 155                 160 


Ile Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn 
                165                 170                 175     


Ala Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn 
            180                 185                 190         


Met Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser 
        195                 200                 205             


Glu Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys 
    210                 215                 220                 


Thr Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala 
225                 230                 235                 240 


Val Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser Gly Ser Gly 
                245                 250                 255     


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
            260                 265                 270         


Pro Gly Pro Met Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu 
        275                 280                 285             


Val Phe Leu Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp 
    290                 295                 300                 


Val Tyr Val Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met 
305                 310                 315                 320 


Val Val Leu Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr 
                325                 330                 335     


Leu Asp Gln Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile 
            340                 345                 350         


Gln Val Lys Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly 
        355                 360                 365             


Gly Glu Val Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp 
    370                 375                 380                 


Gly Ile Trp Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn 
385                 390                 395                 400 


Lys Thr Phe Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr 
                405                 410                 415     


Cys Trp Trp Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys 
            420                 425                 430         


Ser Ser Arg Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala 
        435                 440                 445             


Thr Leu Ser Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr 
    450                 455                 460                 


Ser Val Glu Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser 
465                 470                 475                 480 


Leu Pro Ile Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu 
                485                 490                 495     


Asn Tyr Thr Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro 
            500                 505                 510         


Pro Lys Asn Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu 
        515                 520                 525             


Val Ser Trp Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe 
    530                 535                 540                 


Ser Leu Thr Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys 
545                 550                 555                 560 


Lys Asp Arg Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg 
                565                 570                 575     


Lys Asn Ala Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser 
            580                 585                 590         


Ser Trp Ser Glu Trp Ala Ser Val Pro Cys Ser 
        595                 600             


<210>  63

<211>  253
<212>  PRT
<213>  homo sapiens

<220>
<223>  IL-12a

<400>  63
Met Trp Pro Pro Gly Ser Ala Ser Gln Pro Pro Pro Ser Pro Ala Ala 
1               5                   10                  15      


Ala Thr Gly Leu His Pro Ala Ala Arg Pro Val Ser Leu Gln Cys Arg 
            20                  25                  30          


Leu Ser Met Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr Leu Val 
        35                  40                  45              


Leu Leu Asp His Leu Ser Leu Ala Arg Asn Leu Pro Val Ala Thr Pro 
    50                  55                  60                  


Asp Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg 
65                  70                  75                  80  


Ala Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr 
                85                  90                  95      


Pro Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys 
            100                 105                 110         


Thr Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu 
        115                 120                 125             


Ser Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys 
    130                 135                 140                 


Leu Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser 
145                 150                 155                 160 


Ile Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn 
                165                 170                 175     


Ala Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn 
            180                 185                 190         


Met Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser 
        195                 200                 205             


Glu Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys 
    210                 215                 220                 


Thr Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala 
225                 230                 235                 240 


Val Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser 
                245                 250             


<210>  64

<211>  328
<212>  PRT
<213>  homo sapiens

<220>
<223>  IL-12b

<400>  64
Met Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe Leu 
1               5                   10                  15      


Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr Val 
            20                  25                  30          


Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu 
        35                  40                  45              


Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln 
    50                  55                  60                  


Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys 
65                  70                  75                  80  


Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val 
                85                  90                  95      


Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp 
            100                 105                 110         


Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe 
        115                 120                 125             


Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp 
    130                 135                 140                 


Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg 
145                 150                 155                 160 


Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser 
                165                 170                 175     


Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu 
            180                 185                 190         


Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile 
        195                 200                 205             


Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr 
    210                 215                 220                 


Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn 
225                 230                 235                 240 


Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp 
                245                 250                 255     


Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr 
            260                 265                 270         


Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg 
        275                 280                 285             


Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala 
    290                 295                 300                 


Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser 
305                 310                 315                 320 


Glu Trp Ala Ser Val Pro Cys Ser 
                325             


<210>  65

<211>  378
<212>  PRT
<213>  artifical

<220>
<223>  IgEss_IL-15a-P2A-IgEss_IL-15RA

<400>  65
Gly Thr Gly Ser Ala Thr Met Asp Trp Thr Trp Ile Leu Phe Leu Val 
1               5                   10                  15      


Ala Ala Ala Thr Arg Val His Ser Gly Ile His Val Phe Ile Leu Gly 
            20                  25                  30          


Cys Phe Ser Ala Gly Leu Pro Lys Thr Glu Ala Asn Trp Val Asn Val 
        35                  40                  45              


Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile Gln Ser Met His Ile 
    50                  55                  60                  


Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His Pro Ser Cys Lys Val 
65                  70                  75                  80  


Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln Val Ile Ser Leu Glu 
                85                  90                  95      


Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu Asn Leu Ile Ile Leu 
            100                 105                 110         


Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val Thr Glu Ser Gly Cys 
        115                 120                 125             


Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile Lys Glu Phe Leu Gln 
    130                 135                 140                 


Ser Phe Val His Ile Val Gln Met Phe Ile Asn Thr Ser Gly Ser Gly 
145                 150                 155                 160 


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
                165                 170                 175     


Pro Gly Pro Gly Thr Gly Ser Ala Thr Met Asp Trp Thr Trp Ile Leu 
            180                 185                 190         


Phe Leu Val Ala Ala Ala Thr Arg Val His Ser Ile Thr Cys Pro Pro 
        195                 200                 205             


Pro Met Ser Val Glu His Ala Asp Ile Trp Val Lys Ser Tyr Ser Leu 
    210                 215                 220                 


Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly Phe Lys Arg Lys Ala 
225                 230                 235                 240 


Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn Lys Ala Thr Asn Val 
                245                 250                 255     


Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile Arg Asp Pro Ala Leu 
            260                 265                 270         


Val His Gln Arg Pro Ala Pro Pro Ser Thr Val Thr Thr Ala Gly Val 
        275                 280                 285             


Thr Pro Gln Pro Glu Ser Leu Ser Pro Ser Gly Lys Glu Pro Ala Ala 
    290                 295                 300                 


Ser Ser Pro Ser Ser Asn Asn Thr Ala Ala Thr Thr Ala Ala Ile Val 
305                 310                 315                 320 


Pro Gly Ser Gln Leu Met Pro Ser Lys Ser Pro Ser Thr Gly Thr Thr 
                325                 330                 335     


Glu Ile Ser Ser His Glu Ser Ser His Gly Thr Pro Ser Gln Thr Thr 
            340                 345                 350         


Ala Lys Asn Trp Glu Leu Thr Ala Ser Ala Ser His Gln Pro Pro Gly 
        355                 360                 365             


Val Tyr Pro Gln Gly His Ser Asp Thr Thr 
    370                 375             


<210>  66

<211>  133
<212>  PRT
<213>  homo sapiens

<220>
<223>  IL-15a

<400>  66
Gly Ile His Val Phe Ile Leu Gly Cys Phe Ser Ala Gly Leu Pro Lys 
1               5                   10                  15      


Thr Glu Ala Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu 
            20                  25                  30          


Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser 
        35                  40                  45              


Asp Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu 
    50                  55                  60                  


Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp 
65                  70                  75                  80  


Thr Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn 
                85                  90                  95      


Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu 
            100                 105                 110         


Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met 
        115                 120                 125             


Phe Ile Asn Thr Ser 
    130             


<210>  67

<211>  175
<212>  PRT
<213>  homo sapiens

<220>
<223>  IL-15RA

<400>  67
Ile Thr Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp Val 
1               5                   10                  15      


Lys Ser Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly 
            20                  25                  30          


Phe Lys Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn 
        35                  40                  45              


Lys Ala Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile 
    50                  55                  60                  


Arg Asp Pro Ala Leu Val His Gln Arg Pro Ala Pro Pro Ser Thr Val 
65                  70                  75                  80  


Thr Thr Ala Gly Val Thr Pro Gln Pro Glu Ser Leu Ser Pro Ser Gly 
                85                  90                  95      


Lys Glu Pro Ala Ala Ser Ser Pro Ser Ser Asn Asn Thr Ala Ala Thr 
            100                 105                 110         


Thr Ala Ala Ile Val Pro Gly Ser Gln Leu Met Pro Ser Lys Ser Pro 
        115                 120                 125             


Ser Thr Gly Thr Thr Glu Ile Ser Ser His Glu Ser Ser His Gly Thr 
    130                 135                 140                 


Pro Ser Gln Thr Thr Ala Lys Asn Trp Glu Leu Thr Ala Ser Ala Ser 
145                 150                 155                 160 


His Gln Pro Pro Gly Val Tyr Pro Gln Gly His Ser Asp Thr Thr 
                165                 170                 175 


<210>  68

<211>  182
<212>  PRT
<213>  homo sapiens

<220>
<223>  IL-18

<400>  68
Met Gly Thr Gly Ser Ala Thr Met Asp Trp Thr Trp Ile Leu Phe Leu 
1               5                   10                  15      


Val Ala Ala Ala Thr Arg Val His Ser Tyr Phe Gly Lys Leu Glu Ser 
            20                  25                  30          


Lys Leu Ser Val Ile Arg Asn Leu Asn Asp Gln Val Leu Phe Ile Asp 
        35                  40                  45              


Gln Gly Asn Arg Pro Leu Phe Glu Asp Met Thr Asp Ser Asp Cys Arg 
    50                  55                  60                  


Asp Asn Ala Pro Arg Thr Ile Phe Ile Ile Ser Met Tyr Lys Asp Ser 
65                  70                  75                  80  


Gln Pro Arg Gly Met Ala Val Thr Ile Ser Val Lys Cys Glu Lys Ile 
                85                  90                  95      


Ser Thr Leu Ser Cys Glu Asn Lys Ile Ile Ser Phe Lys Glu Met Asn 
            100                 105                 110         


Pro Pro Asp Asn Ile Lys Asp Thr Lys Ser Asp Ile Ile Phe Phe Gln 
        115                 120                 125             


Arg Ser Val Pro Gly His Asp Asn Lys Met Gln Phe Glu Ser Ser Ser 
    130                 135                 140                 


Tyr Glu Gly Tyr Phe Leu Ala Cys Glu Lys Glu Arg Asp Leu Phe Lys 
145                 150                 155                 160 


Leu Ile Leu Lys Lys Glu Asp Glu Leu Gly Asp Arg Ser Ile Met Phe 
                165                 170                 175     


Thr Val Gln Asn Glu Asp 
            180         


<210>  69

<211>  405
<212>  PRT
<213>  homo sapiens

<220>
<223>  HYAL1

<400>  69
Met Ala Ala His Leu Leu Pro Ile Cys Ala Leu Phe Leu Thr Leu Leu 
1               5                   10                  15      


Asp Met Ala Gln Gly Phe Arg Gly Pro Leu Leu Pro Asn Arg Pro Phe 
            20                  25                  30          


Thr Thr Val Trp Asn Ala Asn Thr Gln Trp Cys Leu Glu Arg His Gly 
        35                  40                  45              


Val Asp Val Asp Val Ser Val Phe Asp Val Val Ala Asn Pro Gly Gln 
    50                  55                  60                  


Thr Phe Arg Gly Pro Asp Met Thr Ile Phe Tyr Ser Ser Gln Leu Gly 
65                  70                  75                  80  


Thr Tyr Pro Tyr Tyr Thr Pro Thr Gly Glu Pro Val Phe Gly Gly Leu 
                85                  90                  95      


Pro Gln Asn Ala Ser Leu Ile Ala His Leu Ala Arg Thr Phe Gln Asp 
            100                 105                 110         


Ile Leu Ala Ala Ile Pro Ala Pro Asp Phe Ser Gly Leu Ala Val Ile 
        115                 120                 125             


Asp Trp Glu Ala Trp Arg Pro Arg Trp Ala Phe Asn Trp Asp Thr Lys 
    130                 135                 140                 


Asp Ile Tyr Arg Gln Arg Ser Arg Ala Leu Val Gln Ala Gln His Pro 
145                 150                 155                 160 


Asp Trp Pro Ala Pro Gln Val Glu Ala Val Ala Gln Asp Gln Phe Gln 
                165                 170                 175     


Gly Ala Ala Arg Ala Trp Met Ala Gly Thr Leu Gln Leu Gly Arg Ala 
            180                 185                 190         


Leu Arg Pro Arg Gly Leu Trp Gly Phe Tyr Gly Phe Pro Asp Cys Tyr 
        195                 200                 205             


Asn Tyr Asp Phe Leu Ser Pro Asn Tyr Thr Gly Gln Cys Pro Ser Gly 
    210                 215                 220                 


Ile Arg Ala Gln Asn Asp Gln Leu Gly Trp Leu Trp Gly Gln Ser Arg 
225                 230                 235                 240 


Ala Leu Tyr Pro Ser Ile Tyr Met Pro Ala Val Leu Glu Gly Thr Gly 
                245                 250                 255     


Lys Ser Gln Met Tyr Val Gln His Arg Val Ala Glu Ala Phe Arg Val 
            260                 265                 270         


Ala Val Ala Ala Gly Asp Pro Asn Leu Pro Val Leu Pro Tyr Val Gln 
        275                 280                 285             


Ile Phe Tyr Asp Thr Thr Asn His Phe Leu Pro Leu Glu Ser Cys Gln 
    290                 295                 300                 


Ala Ile Lys Glu Tyr Met Asp Thr Thr Leu Gly Pro Phe Ile Leu Asn 
305                 310                 315                 320 


Val Thr Ser Gly Ala Leu Leu Cys Ser Gln Ala Leu Cys Ser Gly His 
                325                 330                 335     


Gly Arg Cys Val Arg Arg Thr Ser His Pro Lys Ala Leu Leu Leu Leu 
            340                 345                 350         


Asn Pro Ala Ser Phe Ser Ile Gln Leu Thr Pro Gly Gly Gly Pro Leu 
        355                 360                 365             


Ser Leu Arg Gly Ala Leu Ser Leu Glu Asp Gln Ala Gln Met Ala Val 
    370                 375                 380                 


Glu Phe Lys Cys Arg Cys Tyr Pro Gly Trp Gln Ala Pro Trp Cys Glu 
385                 390                 395                 400 


Arg Lys Ser Met Trp 
                405 


<210>  70

<211>  473
<212>  PRT
<213>  homo sapiens

<220>
<223>  HYAL2

<400>  70
Met Arg Ala Gly Pro Gly Pro Thr Val Thr Leu Ala Leu Val Leu Ala 
1               5                   10                  15      


Val Ser Trp Ala Met Glu Leu Lys Pro Thr Ala Pro Pro Ile Phe Thr 
            20                  25                  30          


Gly Arg Pro Phe Val Val Ala Trp Asp Val Pro Thr Gln Asp Cys Gly 
        35                  40                  45              


Pro Arg Leu Lys Val Pro Leu Asp Leu Asn Ala Phe Asp Val Gln Ala 
    50                  55                  60                  


Ser Pro Asn Glu Gly Phe Val Asn Gln Asn Ile Thr Ile Phe Tyr Arg 
65                  70                  75                  80  


Asp Arg Leu Gly Leu Tyr Pro Arg Phe Asp Ser Ala Gly Arg Ser Val 
                85                  90                  95      


His Gly Gly Val Pro Gln Asn Val Ser Leu Trp Ala His Arg Lys Met 
            100                 105                 110         


Leu Gln Lys Arg Val Glu His Tyr Ile Arg Thr Gln Glu Ser Ala Gly 
        115                 120                 125             


Leu Ala Val Ile Asp Trp Glu Asp Trp Arg Pro Val Trp Val Arg Asn 
    130                 135                 140                 


Trp Gln Asp Lys Asp Val Tyr Arg Arg Leu Ser Arg Gln Leu Val Ala 
145                 150                 155                 160 


Ser Arg His Pro Asp Trp Pro Pro Asp Arg Ile Val Lys Gln Ala Gln 
                165                 170                 175     


Tyr Glu Phe Glu Phe Ala Ala Gln Gln Phe Met Leu Glu Thr Leu Arg 
            180                 185                 190         


Tyr Val Lys Ala Val Arg Pro Arg His Leu Trp Gly Phe Tyr Leu Phe 
        195                 200                 205             


Pro Asp Cys Tyr Asn His Asp Tyr Val Gln Asn Trp Glu Ser Tyr Thr 
    210                 215                 220                 


Gly Arg Cys Pro Asp Val Glu Val Ala Arg Asn Asp Gln Leu Ala Trp 
225                 230                 235                 240 


Leu Trp Ala Glu Ser Thr Ala Leu Phe Pro Ser Val Tyr Leu Asp Glu 
                245                 250                 255     


Thr Leu Ala Ser Ser Arg His Gly Arg Asn Phe Val Ser Phe Arg Val 
            260                 265                 270         


Gln Glu Ala Leu Arg Val Ala Arg Thr His His Ala Asn His Ala Leu 
        275                 280                 285             


Pro Val Tyr Val Phe Thr Arg Pro Thr Tyr Ser Arg Arg Leu Thr Gly 
    290                 295                 300                 


Leu Ser Glu Met Asp Leu Ile Ser Thr Ile Gly Glu Ser Ala Ala Leu 
305                 310                 315                 320 


Gly Ala Ala Gly Val Ile Leu Trp Gly Asp Ala Gly Tyr Thr Thr Ser 
                325                 330                 335     


Thr Glu Thr Cys Gln Tyr Leu Lys Asp Tyr Leu Thr Arg Leu Leu Val 
            340                 345                 350         


Pro Tyr Val Val Asn Val Ser Trp Ala Thr Gln Tyr Cys Ser Arg Ala 
        355                 360                 365             


Gln Cys His Gly His Gly Arg Cys Val Arg Arg Asn Pro Ser Ala Ser 
    370                 375                 380                 


Thr Phe Leu His Leu Ser Thr Asn Ser Phe Arg Leu Val Pro Gly His 
385                 390                 395                 400 


Ala Pro Gly Glu Pro Gln Leu Arg Pro Val Gly Glu Leu Ser Trp Ala 
                405                 410                 415     


Asp Ile Asp His Leu Gln Thr His Phe Arg Cys Gln Cys Tyr Leu Gly 
            420                 425                 430         


Trp Ser Gly Glu Gln Cys Gln Trp Asp His Arg Gln Ala Ala Gly Gly 
        435                 440                 445             


Ala Ser Glu Ala Trp Ala Gly Ser His Leu Thr Ser Leu Leu Ala Leu 
    450                 455                 460                 


Ala Ala Leu Ala Phe Thr Trp Thr Leu 
465                 470             


<210>  71

<211>  511
<212>  PRT
<213>  homo sapiens

<220>
<223>  SPAM1

<400>  71
Met Gly Val Leu Lys Phe Lys His Ile Phe Phe Arg Ser Phe Val Lys 
1               5                   10                  15      


Ser Ser Gly Val Ser Gln Ile Val Phe Thr Phe Leu Leu Ile Pro Cys 
            20                  25                  30          


Cys Leu Thr Leu Asn Phe Arg Ala Pro Pro Val Ile Pro Asn Val Pro 
        35                  40                  45              


Phe Leu Trp Ala Trp Asn Ala Pro Ser Glu Phe Cys Leu Gly Lys Phe 
    50                  55                  60                  


Asp Glu Pro Leu Asp Met Ser Leu Phe Ser Phe Ile Gly Ser Pro Arg 
65                  70                  75                  80  


Ile Asn Ala Thr Gly Gln Gly Val Thr Ile Phe Tyr Val Asp Arg Leu 
                85                  90                  95      


Gly Tyr Tyr Pro Tyr Ile Asp Ser Ile Thr Gly Val Thr Val Asn Gly 
            100                 105                 110         


Gly Ile Pro Gln Lys Ile Ser Leu Gln Asp His Leu Asp Lys Ala Lys 
        115                 120                 125             


Lys Asp Ile Thr Phe Tyr Met Pro Val Asp Asn Leu Gly Met Ala Val 
    130                 135                 140                 


Ile Asp Trp Glu Glu Trp Arg Pro Thr Trp Ala Arg Asn Trp Lys Pro 
145                 150                 155                 160 


Lys Asp Val Tyr Lys Asn Arg Ser Ile Glu Leu Val Gln Gln Gln Asn 
                165                 170                 175     


Val Gln Leu Ser Leu Thr Glu Ala Thr Glu Lys Ala Lys Gln Glu Phe 
            180                 185                 190         


Glu Lys Ala Gly Lys Asp Phe Leu Val Glu Thr Ile Lys Leu Gly Lys 
        195                 200                 205             


Leu Leu Arg Pro Asn His Leu Trp Gly Tyr Tyr Leu Phe Pro Asp Cys 
    210                 215                 220                 


Tyr Asn His His Tyr Lys Lys Pro Gly Tyr Asn Gly Ser Cys Phe Asn 
225                 230                 235                 240 


Val Glu Ile Lys Arg Asn Asp Asp Leu Ser Trp Leu Trp Asn Glu Ser 
                245                 250                 255     


Thr Ala Leu Tyr Pro Ser Ile Tyr Leu Asn Thr Gln Gln Ser Pro Val 
            260                 265                 270         


Ala Ala Thr Leu Tyr Val Arg Asn Arg Val Arg Glu Ala Ile Arg Val 
        275                 280                 285             


Ser Lys Ile Pro Asp Ala Lys Ser Pro Leu Pro Val Phe Ala Tyr Thr 
    290                 295                 300                 


Arg Ile Val Phe Thr Asp Gln Val Leu Lys Phe Leu Ser Gln Asp Glu 
305                 310                 315                 320 


Leu Val Tyr Thr Phe Gly Glu Thr Val Ala Leu Gly Ala Ser Gly Ile 
                325                 330                 335     


Val Ile Trp Gly Thr Leu Ser Ile Met Arg Ser Met Lys Ser Cys Leu 
            340                 345                 350         


Leu Leu Asp Asn Tyr Met Glu Thr Ile Leu Asn Pro Tyr Ile Ile Asn 
        355                 360                 365             


Val Thr Leu Ala Ala Lys Met Cys Ser Gln Val Leu Cys Gln Glu Gln 
    370                 375                 380                 


Gly Val Cys Ile Arg Lys Asn Trp Asn Ser Ser Asp Tyr Leu His Leu 
385                 390                 395                 400 


Asn Pro Asp Asn Phe Ala Ile Gln Leu Glu Lys Gly Gly Lys Phe Thr 
                405                 410                 415     


Val Arg Gly Lys Pro Thr Leu Glu Asp Leu Glu Gln Phe Ser Glu Lys 
            420                 425                 430         


Phe Tyr Cys Ser Cys Tyr Ser Thr Leu Ser Cys Lys Glu Lys Ala Asp 
        435                 440                 445             


Val Lys Asp Thr Asp Ala Val Asp Val Cys Ile Ala Asp Gly Val Cys 
    450                 455                 460                 


Ile Asp Ala Phe Leu Lys Pro Pro Met Glu Thr Glu Glu Pro Gln Ile 
465                 470                 475                 480 


Phe Tyr Asn Ala Ser Pro Ser Thr Leu Ser Ala Thr Met Phe Ile Trp 
                485                 490                 495     


Arg Leu Glu Val Trp Asp Gln Gly Ile Ser Arg Ile Gly Phe Phe 
            500                 505                 510     


<210>  72

<211>  569
<212>  PRT
<213>  homo sapiens

<220>
<223>  GPI1

<400>  72
Met Val Ala Leu Cys Ser Leu Gln His Leu Gly Ser Ser Asp Pro Arg 
1               5                   10                  15      


Ala Leu Pro Thr Leu Pro Thr Ala Thr Ser Gly Gln Arg Pro Ala Lys 
            20                  25                  30          


Arg Arg Arg Lys Ser Pro Ala Met Ala Ala Leu Thr Arg Asp Pro Gln 
        35                  40                  45              


Phe Gln Lys Leu Gln Gln Trp Tyr Arg Glu His Arg Ser Glu Leu Asn 
    50                  55                  60                  


Leu Arg Arg Leu Phe Asp Ala Asn Lys Asp Arg Phe Asn His Phe Ser 
65                  70                  75                  80  


Leu Thr Leu Asn Thr Asn His Gly His Ile Leu Val Asp Tyr Ser Lys 
                85                  90                  95      


Asn Leu Val Thr Glu Asp Val Met Arg Met Leu Val Asp Leu Ala Lys 
            100                 105                 110         


Ser Arg Gly Val Glu Ala Ala Arg Glu Arg Met Phe Asn Gly Glu Lys 
        115                 120                 125             


Ile Asn Tyr Thr Glu Gly Arg Ala Val Leu His Val Ala Leu Arg Asn 
    130                 135                 140                 


Arg Ser Asn Thr Pro Ile Leu Val Asp Gly Lys Asp Val Met Pro Glu 
145                 150                 155                 160 


Val Asn Lys Val Leu Asp Lys Met Lys Ser Phe Cys Gln Gly Pro Leu 
                165                 170                 175     


Met Val Thr Glu Ala Leu Lys Pro Tyr Ser Ser Gly Gly Pro Arg Val 
            180                 185                 190         


Trp Tyr Val Ser Asn Ile Asp Gly Thr His Ile Ala Lys Thr Leu Ala 
        195                 200                 205             


Gln Leu Asn Pro Glu Ser Ser Leu Phe Ile Ile Ala Ser Lys Thr Phe 
    210                 215                 220                 


Thr Thr Gln Glu Thr Ile Thr Asn Ala Glu Thr Ala Lys Glu Trp Phe 
225                 230                 235                 240 


Leu Gln Ala Ala Lys Asp Pro Ser Ala Val Ala Lys His Phe Val Ala 
                245                 250                 255     


Leu Ser Thr Asn Thr Thr Lys Val Lys Glu Phe Gly Ile Asp Pro Gln 
            260                 265                 270         


Asn Met Phe Glu Phe Trp Asp Trp Val Gly Gly Arg Tyr Ser Leu Trp 
        275                 280                 285             


Ser Ala Ile Gly Leu Ser Ile Ala Leu His Val Gly Phe Asp Asn Phe 
    290                 295                 300                 


Glu Gln Leu Leu Ser Gly Ala His Trp Met Asp Gln His Phe Arg Thr 
305                 310                 315                 320 


Thr Pro Leu Glu Lys Asn Ala Pro Val Leu Leu Ala Leu Leu Gly Ile 
                325                 330                 335     


Trp Tyr Ile Asn Cys Phe Gly Cys Glu Thr His Ala Met Leu Pro Tyr 
            340                 345                 350         


Asp Gln Tyr Leu His Arg Phe Ala Ala Tyr Phe Gln Gln Gly Asp Met 
        355                 360                 365             


Glu Ser Asn Gly Lys Tyr Ile Thr Lys Ser Gly Thr Arg Val Asp His 
    370                 375                 380                 


Gln Thr Gly Pro Ile Val Trp Gly Glu Pro Gly Thr Asn Gly Gln His 
385                 390                 395                 400 


Ala Phe Tyr Gln Leu Ile His Gln Gly Thr Lys Met Ile Pro Cys Asp 
                405                 410                 415     


Phe Leu Ile Pro Val Gln Thr Gln His Pro Ile Arg Lys Gly Leu His 
            420                 425                 430         


His Lys Ile Leu Leu Ala Asn Phe Leu Ala Gln Thr Glu Ala Leu Met 
        435                 440                 445             


Arg Gly Lys Ser Thr Glu Glu Ala Arg Lys Glu Leu Gln Ala Ala Gly 
    450                 455                 460                 


Lys Ser Pro Glu Asp Leu Glu Arg Leu Leu Pro His Lys Val Phe Glu 
465                 470                 475                 480 


Gly Asn Arg Pro Thr Asn Ser Ile Val Phe Thr Lys Leu Thr Pro Phe 
                485                 490                 495     


Met Leu Gly Ala Leu Val Ala Met Tyr Glu His Lys Ile Phe Val Gln 
            500                 505                 510         


Gly Ile Ile Trp Asp Ile Asn Ser Phe Asp Gln Trp Gly Val Glu Leu 
        515                 520                 525             


Gly Lys Gln Leu Ala Lys Lys Ile Glu Pro Glu Leu Asp Gly Ser Ala 
    530                 535                 540                 


Gln Val Thr Ser His Asp Ala Ser Thr Asn Gly Leu Ile Asn Phe Ile 
545                 550                 555                 560 


Lys Gln Gln Arg Glu Ala Arg Val Gln 
                565                 


<210>  73

<211>  622
<212>  PRT
<213>  homo sapiens

<220>
<223>  PCK1

<400>  73
Met Pro Pro Gln Leu Gln Asn Gly Leu Asn Leu Ser Ala Lys Val Val 
1               5                   10                  15      


Gln Gly Ser Leu Asp Ser Leu Pro Gln Ala Val Arg Glu Phe Leu Glu 
            20                  25                  30          


Asn Asn Ala Glu Leu Cys Gln Pro Asp His Ile His Ile Cys Asp Gly 
        35                  40                  45              


Ser Glu Glu Glu Asn Gly Arg Leu Leu Gly Gln Met Glu Glu Glu Gly 
    50                  55                  60                  


Ile Leu Arg Arg Leu Lys Lys Tyr Asp Asn Cys Trp Leu Ala Leu Thr 
65                  70                  75                  80  


Asp Pro Arg Asp Val Ala Arg Ile Glu Ser Lys Thr Val Ile Val Thr 
                85                  90                  95      


Gln Glu Gln Arg Asp Thr Val Pro Ile Pro Lys Thr Gly Leu Ser Gln 
            100                 105                 110         


Leu Gly Arg Trp Met Ser Glu Glu Asp Phe Glu Lys Ala Phe Asn Ala 
        115                 120                 125             


Arg Phe Pro Gly Cys Met Lys Gly Arg Thr Met Tyr Val Ile Pro Phe 
    130                 135                 140                 


Ser Met Gly Pro Leu Gly Ser Pro Leu Ser Lys Ile Gly Ile Glu Leu 
145                 150                 155                 160 


Thr Asp Ser Pro Tyr Val Val Ala Ser Met Arg Ile Met Thr Arg Met 
                165                 170                 175     


Gly Thr Pro Val Leu Glu Ala Val Gly Asp Gly Glu Phe Val Lys Cys 
            180                 185                 190         


Leu His Ser Val Gly Cys Pro Leu Pro Leu Gln Lys Pro Leu Val Asn 
        195                 200                 205             


Asn Trp Pro Cys Asn Pro Glu Leu Thr Leu Ile Ala His Leu Pro Asp 
    210                 215                 220                 


Arg Arg Glu Ile Ile Ser Phe Gly Ser Gly Tyr Gly Gly Asn Ser Leu 
225                 230                 235                 240 


Leu Gly Lys Lys Cys Phe Ala Leu Arg Met Ala Ser Arg Leu Ala Lys 
                245                 250                 255     


Glu Glu Gly Trp Leu Ala Glu His Met Leu Ile Leu Gly Ile Thr Asn 
            260                 265                 270         


Pro Glu Gly Glu Lys Lys Tyr Leu Ala Ala Ala Phe Pro Ser Ala Cys 
        275                 280                 285             


Gly Lys Thr Asn Leu Ala Met Met Asn Pro Ser Leu Pro Gly Trp Lys 
    290                 295                 300                 


Val Glu Cys Val Gly Asp Asp Ile Ala Trp Met Lys Phe Asp Ala Gln 
305                 310                 315                 320 


Gly His Leu Arg Ala Ile Asn Pro Glu Asn Gly Phe Phe Gly Val Ala 
                325                 330                 335     


Pro Gly Thr Ser Val Lys Thr Asn Pro Asn Ala Ile Lys Thr Ile Gln 
            340                 345                 350         


Lys Asn Thr Ile Phe Thr Asn Val Ala Glu Thr Ser Asp Gly Gly Val 
        355                 360                 365             


Tyr Trp Glu Gly Ile Asp Glu Pro Leu Ala Ser Gly Val Thr Ile Thr 
    370                 375                 380                 


Ser Trp Lys Asn Lys Glu Trp Ser Ser Glu Asp Gly Glu Pro Cys Ala 
385                 390                 395                 400 


His Pro Asn Ser Arg Phe Cys Thr Pro Ala Ser Gln Cys Pro Ile Ile 
                405                 410                 415     


Asp Ala Ala Trp Glu Ser Pro Glu Gly Val Pro Ile Glu Gly Ile Ile 
            420                 425                 430         


Phe Gly Gly Arg Arg Pro Ala Gly Val Pro Leu Val Tyr Glu Ala Leu 
        435                 440                 445             


Ser Trp Gln His Gly Val Phe Val Gly Ala Ala Met Arg Ser Glu Ala 
    450                 455                 460                 


Thr Ala Ala Ala Glu His Lys Gly Lys Ile Ile Met His Asp Pro Phe 
465                 470                 475                 480 


Ala Met Arg Pro Phe Phe Gly Tyr Asn Phe Gly Lys Tyr Leu Ala His 
                485                 490                 495     


Trp Leu Ser Met Ala Gln His Pro Ala Ala Lys Leu Pro Lys Ile Phe 
            500                 505                 510         


His Val Asn Trp Phe Arg Lys Asp Lys Glu Gly Lys Phe Leu Trp Pro 
        515                 520                 525             


Gly Phe Gly Glu Asn Ser Arg Val Leu Glu Trp Met Phe Asn Arg Ile 
    530                 535                 540                 


Asp Gly Lys Ala Ser Thr Lys Leu Thr Pro Ile Gly Tyr Ile Pro Lys 
545                 550                 555                 560 


Glu Asp Ala Leu Asn Leu Lys Gly Leu Gly His Ile Asn Met Met Glu 
                565                 570                 575     


Leu Phe Ser Ile Ser Lys Glu Phe Trp Glu Lys Glu Val Glu Asp Ile 
            580                 585                 590         


Glu Lys Tyr Leu Glu Asp Gln Val Asn Ala Asp Leu Pro Cys Glu Ile 
        595                 600                 605             


Glu Arg Glu Ile Leu Ala Leu Lys Gln Arg Ile Ser Gln Met 
    610                 615                 620         


<210>  74

<211>  332
<212>  PRT
<213>  homo sapiens

<220>
<223>  LDHA

<400>  74
Met Ala Thr Leu Lys Asp Gln Leu Ile Tyr Asn Leu Leu Lys Glu Glu 
1               5                   10                  15      


Gln Thr Pro Gln Asn Lys Ile Thr Val Val Gly Val Gly Ala Val Gly 
            20                  25                  30          


Met Ala Cys Ala Ile Ser Ile Leu Met Lys Asp Leu Ala Asp Glu Leu 
        35                  40                  45              


Ala Leu Val Asp Val Ile Glu Asp Lys Leu Lys Gly Glu Met Met Asp 
    50                  55                  60                  


Leu Gln His Gly Ser Leu Phe Leu Arg Thr Pro Lys Ile Val Ser Gly 
65                  70                  75                  80  


Lys Asp Tyr Asn Val Thr Ala Asn Ser Lys Leu Val Ile Ile Thr Ala 
                85                  90                  95      


Gly Ala Arg Gln Gln Glu Gly Glu Ser Arg Leu Asn Leu Val Gln Arg 
            100                 105                 110         


Asn Val Asn Ile Phe Lys Phe Ile Ile Pro Asn Val Val Lys Tyr Ser 
        115                 120                 125             


Pro Asn Cys Lys Leu Leu Ile Val Ser Asn Pro Val Asp Ile Leu Thr 
    130                 135                 140                 


Tyr Val Ala Trp Lys Ile Ser Gly Phe Pro Lys Asn Arg Val Ile Gly 
145                 150                 155                 160 


Ser Gly Cys Asn Leu Asp Ser Ala Arg Phe Arg Tyr Leu Met Gly Glu 
                165                 170                 175     


Arg Leu Gly Val His Pro Leu Ser Cys His Gly Trp Val Leu Gly Glu 
            180                 185                 190         


His Gly Asp Ser Ser Val Pro Val Trp Ser Gly Met Asn Val Ala Gly 
        195                 200                 205             


Val Ser Leu Lys Thr Leu His Pro Asp Leu Gly Thr Asp Lys Asp Lys 
    210                 215                 220                 


Glu Gln Trp Lys Glu Val His Lys Gln Val Val Glu Ser Ala Tyr Glu 
225                 230                 235                 240 


Val Ile Lys Leu Lys Gly Tyr Thr Ser Trp Ala Ile Gly Leu Ser Val 
                245                 250                 255     


Ala Asp Leu Ala Glu Ser Ile Met Lys Asn Leu Arg Arg Val His Pro 
            260                 265                 270         


Val Ser Thr Met Ile Lys Gly Leu Tyr Gly Ile Lys Asp Asp Val Phe 
        275                 280                 285             


Leu Ser Val Pro Cys Ile Leu Gly Gln Asn Gly Ile Ser Asp Leu Val 
    290                 295                 300                 


Lys Val Thr Leu Thr Ser Glu Glu Glu Ala Arg Leu Lys Lys Ser Ala 
305                 310                 315                 320 


Asp Thr Leu Trp Gly Ile Gln Lys Glu Leu Gln Phe 
                325                 330         


<210>  75

<211>  925
<212>  PRT
<213>  artificial

<220>
<223>  TRAC TALEN Left

<400>  75
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Ile Ala Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
        275                 280                 285             


Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
305                 310                 315                 320 


Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
            340                 345                 350         


Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
545                 550                 555                 560 


Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
            580                 585                 590         


Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln 
        595                 600                 605             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
    610                 615                 620                 


Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly 
625                 630                 635                 640 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                645                 650                 655     


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
            660                 665                 670         


Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro 
        675                 680                 685             


Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala 
    690                 695                 700                 


Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly 
705                 710                 715                 720 


Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys 
                725                 730                 735     


Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile 
            740                 745                 750         


Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu 
        755                 760                 765             


Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys 
    770                 775                 780                 


His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly 
785                 790                 795                 800 


Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly 
                805                 810                 815     


Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val 
            820                 825                 830         


Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp 
        835                 840                 845             


Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser 
    850                 855                 860                 


Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His 
865                 870                 875                 880 


Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile 
                885                 890                 895     


Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg 
            900                 905                 910         


Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
        915                 920                 925 


<210>  76

<211>  925
<212>  PRT
<213>  artificial

<220>
<223>  TRAC TALEN Right

<400>  76
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Ile Ala Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
        275                 280                 285             


Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
305                 310                 315                 320 


Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
            340                 345                 350         


Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
545                 550                 555                 560 


Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
            580                 585                 590         


Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
        595                 600                 605             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
    610                 615                 620                 


Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
625                 630                 635                 640 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                645                 650                 655     


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
            660                 665                 670         


Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro 
        675                 680                 685             


Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala 
    690                 695                 700                 


Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly 
705                 710                 715                 720 


Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys 
                725                 730                 735     


Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile 
            740                 745                 750         


Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu 
        755                 760                 765             


Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys 
    770                 775                 780                 


His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly 
785                 790                 795                 800 


Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly 
                805                 810                 815     


Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val 
            820                 825                 830         


Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp 
        835                 840                 845             


Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser 
    850                 855                 860                 


Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His 
865                 870                 875                 880 


Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile 
                885                 890                 895     


Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg 
            900                 905                 910         


Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
        915                 920                 925 


<210>  77

<211>  925
<212>  PRT
<213>  artificial

<220>
<223>  PD1 TALEN Left

<400>  77
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Ile Ala Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Lys Leu Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
        275                 280                 285             


Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
305                 310                 315                 320 


Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
            340                 345                 350         


Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Tyr Lys Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
545                 550                 555                 560 


Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
            580                 585                 590         


Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
        595                 600                 605             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
    610                 615                 620                 


Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
625                 630                 635                 640 


Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln 
                645                 650                 655     


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
            660                 665                 670         


Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro 
        675                 680                 685             


Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala 
    690                 695                 700                 


Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly 
705                 710                 715                 720 


Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys 
                725                 730                 735     


Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile 
            740                 745                 750         


Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu 
        755                 760                 765             


Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys 
    770                 775                 780                 


His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly 
785                 790                 795                 800 


Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly 
                805                 810                 815     


Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val 
            820                 825                 830         


Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp 
        835                 840                 845             


Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser 
    850                 855                 860                 


Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His 
865                 870                 875                 880 


Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile 
                885                 890                 895     


Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg 
            900                 905                 910         


Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
        915                 920                 925 


<210>  78

<211>  924
<212>  PRT
<213>  artificial

<220>
<223>  PD1 TALEN Right

<400>  78
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Ile Ala Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
        275                 280                 285             


Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
305                 310                 315                 320 


Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
            340                 345                 350         


Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
545                 550                 555                 560 


Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly Lys Gln Ala Leu Glu 
                565                 570                 575     


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
            580                 585                 590         


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala 
        595                 600                 605             


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly 
    610                 615                 620                 


Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys 
625                 630                 635                 640 


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
                645                 650                 655     


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly 
            660                 665                 670         


Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro Asp 
        675                 680                 685             


Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala Cys 
    690                 695                 700                 


Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly Asp 
705                 710                 715                 720 


Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys Lys 
                725                 730                 735     


Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile Glu 
            740                 745                 750         


Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu Met 
        755                 760                 765             


Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys His 
    770                 775                 780                 


Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly Ser 
785                 790                 795                 800 


Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly Gly 
                805                 810                 815     


Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val Glu 
            820                 825                 830         


Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp Lys 
        835                 840                 845             


Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser Gly 
    850                 855                 860                 


His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His Ile 
865                 870                 875                 880 


Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile Gly 
                885                 890                 895     


Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg Arg 
            900                 905                 910         


Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
        915                 920                 


<210>  79

<211>  925
<212>  PRT
<213>  artificial

<220>
<223>  B2M TALEN Left

<400>  79
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Ile Ala Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
        275                 280                 285             


Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
305                 310                 315                 320 


Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
            340                 345                 350         


Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
545                 550                 555                 560 


Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
            580                 585                 590         


Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
        595                 600                 605             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
    610                 615                 620                 


Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly 
625                 630                 635                 640 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                645                 650                 655     


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
            660                 665                 670         


Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro 
        675                 680                 685             


Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala 
    690                 695                 700                 


Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly 
705                 710                 715                 720 


Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys 
                725                 730                 735     


Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile 
            740                 745                 750         


Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu 
        755                 760                 765             


Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys 
    770                 775                 780                 


His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly 
785                 790                 795                 800 


Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly 
                805                 810                 815     


Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val 
            820                 825                 830         


Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp 
        835                 840                 845             


Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser 
    850                 855                 860                 


Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His 
865                 870                 875                 880 


Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile 
                885                 890                 895     


Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg 
            900                 905                 910         


Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
        915                 920                 925 


<210>  80

<211>  925
<212>  PRT
<213>  artificial

<220>
<223>  B2M TALEN Right

<400>  80
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Ile Ala Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
        275                 280                 285             


Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
305                 310                 315                 320 


Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
            340                 345                 350         


Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
545                 550                 555                 560 


Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
            580                 585                 590         


Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
        595                 600                 605             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
    610                 615                 620                 


Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly 
625                 630                 635                 640 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                645                 650                 655     


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
            660                 665                 670         


Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro 
        675                 680                 685             


Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala 
    690                 695                 700                 


Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly 
705                 710                 715                 720 


Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys 
                725                 730                 735     


Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile 
            740                 745                 750         


Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu 
        755                 760                 765             


Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys 
    770                 775                 780                 


His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly 
785                 790                 795                 800 


Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly 
                805                 810                 815     


Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val 
            820                 825                 830         


Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp 
        835                 840                 845             


Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser 
    850                 855                 860                 


Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His 
865                 870                 875                 880 


Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile 
                885                 890                 895     


Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg 
            900                 905                 910         


Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
        915                 920                 925 


<210>  81

<211>  59
<212>  DNA
<213>  homo sapiens

<220>
<223>  TRAC target sequence

<400>  81
tgatcctctt gtcccacaga tatccagaac cctgaccctg ccgtgtacca gctgagaga      59


<210>  82

<211>  49
<212>  DNA
<213>  homo sapiens

<220>
<223>  PD1 target sequence

<400>  82
tacctctgtg gggccatctc cctggccccc aaggcgcaga tcaaagaga                 49


<210>  83

<211>  47
<212>  DNA
<213>  homo sapiens

<220>
<223>  B2M target sequence

<400>  83
ttagctgtgc tcgcgctact ctctctttct ggcctggagg ctatcca                   47


<210>  84

<211>  3639
<212>  DNA
<213>  artificial

<220>
<223>  AAV6 PD1KO IL-12 (from pCLS30511)

<400>  84
gactccccag acaggccctg gaaccccccc accttctccc cagccctgct cgtggtgacc     60
gaaggggaca acgccacctt cacctgcagc ttctccaaca catcggagag cttcgtgcta    120
aactggtacc gcatgagccc cagcaaccag acggacaagc tggccgcctt ccccgaggac    180
cgcagccagc ccggccagga ctgccgcttc cgtgtcacac aactgcccaa cgggcgtgac    240
ttccacatga gcgtggtcag ggcccggcgc aatgacagcg gcacctacct ctgtggggcc    300
ggttctggcg tgaaacagac tttgaatttt gaccttctca agttggcggg agacgtggag    360
tccaacccag ggcccatgtg gccccctggg tcagcctccc agccaccgcc ctcacctgcc    420
gcggccacag gtctgcatcc agcggctcgc cctgtgtccc tgcagtgccg gctcagcatg    480
tgtccagcgc gcagcctcct ccttgtggct accctggtcc tcctggacca cctcagtttg    540
gccagaaacc tccccgtggc cactccagac ccaggaatgt tcccatgcct tcaccactcc    600
caaaacctgc tgagggccgt cagcaacatg ctccagaagg ccagacaaac tctagaattt    660
tacccttgca cttctgaaga gattgatcat gaagatatca caaaagataa aaccagcaca    720
gtggaggcct gtttaccatt ggaattaacc aagaatgaga gttgcctaaa ttccagagag    780
acctctttca taactaatgg gagttgcctg gcctccagaa agacctcttt tatgatggcc    840
ctgtgcctta gtagtattta tgaagacttg aagatgtacc aggtggagtt caagaccatg    900
aatgcaaagc ttctgatgga tcctaagagg cagatctttc tagatcaaaa catgctggca    960
gttattgatg agctgatgca ggccctgaat ttcaacagtg agactgtgcc acaaaaatcc   1020
tcccttgaag aaccggattt ttataaaact aaaatcaagc tctgcatact tcttcatgct   1080
ttcagaattc gggcagtgac tattgataga gtgatgagct atctgaatgc ttccggaagc   1140
ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct   1200
atgtgtcacc agcagttggt catctcttgg ttttccctgg tttttctggc atctcccctc   1260
gtggccatat gggaactgaa gaaagatgtt tatgtcgtag aattggattg gtatccggat   1320
gcccctggag aaatggtggt cctcacctgt gacacccctg aagaagatgg tatcacctgg   1380
accttggacc agagcagtga ggtcttaggc tctggcaaaa ccctgaccat ccaagtcaaa   1440
gagtttggag atgctggcca gtacacctgt cacaaaggag gcgaggttct aagccattcg   1500
ctcctgctgc ttcacaaaaa ggaagatgga atttggtcca ctgatatttt aaaggaccag   1560
aaagaaccca aaaataagac ctttctaaga tgcgaggcca agaattattc tggacgtttc   1620
acctgctggt ggctgacgac aatcagtact gatttgacat tcagtgtcaa aagcagcaga   1680
ggctcttctg acccccaagg ggtgacgtgc ggagctgcta cactctctgc agagagagtc   1740
agaggggaca acaaggagta tgagtactca gtggagtgcc aggaggacag tgcctgccca   1800
gctgctgagg agagtctgcc cattgaggtc atggtggatg ccgttcacaa gctcaagtat   1860
gaaaactaca ccagcagctt cttcatcagg gacatcatca aacctgaccc acccaagaac   1920
ttgcagctga agccattaaa gaattctcgg caggtggagg tcagctggga gtaccctgac   1980
acctggagta ctccacattc ctacttctcc ctgacattct gcgttcaggt ccagggcaag   2040
agcaagagag aaaagaaaga tagagtcttc acggacaaga cctcagccac ggtcatctgc   2100
cgcaaaaatg ccagcattag cgtgcgggcc caggaccgct actatagctc atcttggagc   2160
gaatgggcat ctgtgccctg cagtgagggc agaggcagcc tgctgacctg cggcgacgtc   2220
gaggagaacc ccgggcccat gggggcaggt gccaccggcc gcgccatgga cgggccgcgc   2280
ctgctgctgt tgctgcttct gggggtgtcc cttggaggtg ccaaggaggc atgccccaca   2340
ggcctgtaca cacacagcgg tgagtgctgc aaagcctgca acctgggcga gggtgtggcc   2400
cagccttgtg gagccaacca gaccgtgtgt gagccctgcc tggacagcgt gacgttctcc   2460
gacgtggtga gcgcgaccga gccgtgcaag ccgtgcaccg agtgcgtggg gctccagagc   2520
atgtcggcgc cgtgcgtgga ggccgatgac gccgtgtgcc gctgcgccta cggctactac   2580
caggatgaga cgactgggcg ctgcgaggcg tgccgcgtgt gcgaggcggg ctcgggcctc   2640
gtgttctcct gccaggacaa gcagaacacc gtgtgcgagg agtgccccga cggcacgtat   2700
tccgacgagg ccaaccacgt ggacccgtgc ctgccctgca ccgtgtgcga ggacaccgag   2760
cgccagctcc gcgagtgcac acgctgggcc gacgccgagt gcgaggagat ccctggccgt   2820
tggattacac ggtccacacc cccagagggc tcggacagca cagcccccag cacccaggag   2880
cctgaggcac ctccagaaca agacctcata gccagcacgg tggcaggtgt ggtgaccaca   2940
gtgatgggca gctcccagcc cgtggtgacc cgaggcacca ccgacaacct catccctgtc   3000
tattgctcca tcctggctgc tgtggttgtg ggtcttgtgg cctacatagc cttcaagagg   3060
tgatctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag   3120
ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact   3180
gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt   3240
ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat   3300
gctggggatg cggtgggctc tatgactagt ggcgaattcg gcgcagatca aagagagcct   3360
gcgggcagag ctcagggtga caggtgcggc ctcggaggcc ccggggcagg ggtgagctga   3420
gccggtcctg gggtgggtgt cccctcctgc acaggatcag gagctccagg gtcgtagggc   3480
agggaccccc cagctccagt ccagggctct gtcctgcacc tggggaatgg tgaccggcat   3540
ctctgtcctc tagctctgga agcaccccag cccctctagt ctgccctcac ccctgaccct   3600
gaccctccac cctgaccccg tcctaacccc tgacctttg                          3639


<210>  85

<211>  2289
<212>  DNA
<213>  artificial

<220>
<223>  AAV6 B2MKO HLA-E (from pCLS31913)

<400>  85
cgcgcacccc agatcggagg gcgccgatgt acagacagca aactcaccca gtctagtgca     60
tgccttctta aacatcacga gactctaaga aaaggaaact gaaaacggga aagtccctct    120
ctctaacctg gcactgcgtc gctggcttgg agacaggtga cggtccctgc gggccttgtc    180
ctgattggct gggcacgcgt ttaatataag tggaggcgtc gcgctggcgg gcattcctga    240
agctgacagc attcgggccg agatgtctcg ctccgtggcc ttagctgtgc tcgcgctact    300
ctctcttagc ggcctcgaag ctgttatggc tccgcggact ttaattttag gtggtggcgg    360
atccggtggt ggcggttctg gtggtggcgg ctccatccag cgtacgccca aaattcaagt    420
ctacagccga catcctgcag agaacggcaa atctaatttc ctgaactgct atgtatcagg    480
ctttcaccct agcgatatag aagtggacct gctgaaaaac ggagagagga tagaaaaggt    540
cgaacacagc gacctctcct tttccaagga ctggagcttt tatcttctgt attatactga    600
atttacaccc acggaaaaag atgagtatgc gtgccgagta aaccacgtca cgctgtcaca    660
gcccaaaata gtaaaatggg atcgcgacat gggtggtggc ggttctggtg gtggcggtag    720
tggcggcgga ggaagcggtg gtggcggttc cggatctcac tccttgaagt atttccacac    780
ttccgtgtcc cggcccggcc gcggggagcc ccgcttcatc tctgtgggct acgtggacga    840
cacccagttc gtgcgcttcg acaacgacgc cgcgagtccg aggatggtgc cgcgggcgcc    900
gtggatggag caggaggggt cagagtattg ggaccgggag acacggagcg ccagggacac    960
cgcacagatt ttccgagtga acctgcggac gctgcgcggc tactacaatc agagcgaggc   1020
cgggtctcac accctgcagt ggatgcatgg ctgcgagctg gggcccgaca ggcgcttcct   1080
ccgcgggtat gaacagttcg cctacgacgg caaggattat ctcaccctga atgaggacct   1140
gcgctcctgg accgcggtgg acacggcggc tcagatctcc gagcaaaagt caaatgatgc   1200
ctctgaggcg gagcaccaga gagcctacct ggaagacaca tgcgtggagt ggctccacaa   1260
atacctggag aaggggaagg agacgctgct tcacctggag cccccaaaga cacacgtgac   1320
tcaccacccc atctctgacc atgaggccac cctgaggtgc tgggctctgg gcttctaccc   1380
tgcggagatc acactgacct ggcagcagga tggggagggc catacccagg acacggagct   1440
cgtggagacc aggcctgcag gggatggaac cttccagaag tgggcagctg tggtggtgcc   1500
ttctggagag gagcagagat acacgtgcca tgtgcagcat gaggggctac ccgagcccgt   1560
caccctgaga tggaagccgg cttcccagcc caccatcccc atcgtgggca tcattgctgg   1620
cctggttctc cttggatctg tggtctctgg agctgtggtt gctgctgtga tatggaggaa   1680
gaagagctca ggtggaaaag gagggagcta ctataaggct gagtggagcg acagtgccca   1740
ggggtctgag tctcacagct tgtaactgtg ccttctagtt gccagccatc tgttgtttgc   1800
ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa   1860
aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg   1920
gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg   1980
ggctctatgt ctctttctgg cctggaggct atccagcgtg agtctctcct accctcccgc   2040
tctggtcctt cctctcccgc tctgcaccct ctgtggccct cgctgtgctc tctcgctccg   2100
tgacttccct tctccaagtt ctccttggtg gcccgccgtg gggctagtcc agggctggat   2160
ctcggggaag cggcggggtg gcctgggagt ggggaagggg gtgcgcaccc gggacgcgcg   2220
ctacttgccc ctttcggcgg ggagcagggg agacctttgg cctacggcga cgggagggtc   2280
gggacaaag                                                           2289


<210>  86

<211>  49
<212>  DNA
<213>  artificial

<220>
<223>  Target Sequence into TGFbetaRII T003387

<400>  86
ttttgtttcc ccatcagaat ataacaccag caatcctgac ttgttgcta                 49


<210>  87

<211>  49
<212>  DNA
<213>  artificial

<220>
<223>  Target Sequence into TGFbetaRII T003401

<400>  87
tccctatgag gagtatgcct cttggaagac agagaaggac atcttctca                 49


<210>  88

<211>  49
<212>  DNA
<213>  artificial

<220>
<223>  Target Sequence into TGFbetaRII T003400

<400>  88
tggcagtcaa gatctttccc tatgaggagt atgcctcttg gaagacaga                 49


<210>  89

<211>  49
<212>  DNA
<213>  artificial

<220>
<223>  Target Sequence into TGFbetaRII T003405

<400>  89
tgtgggaggc ccaagatgcc catcgtgcac agggacctca agagctcca                 49


<210>  90

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  90
acagtgatca cactccatgt ggg                                             23


<210>  91

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  91
gcagaagctg agttcaacct ggg                                             23


<210>  92

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  92
aggttaggtc gttcttcacg agg                                             23


<210>  93

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  93
aaagcgacct ttccccacca ggg                                             23


<210>  94

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  94
tggatgacct ggctaacagt ggg                                             23


<210>  95

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  95
cctgggaaac cggcaagacg cgg                                             23


<210>  96

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  96
acagatatgg caactcccag tgg                                             23


<210>  97

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  97
gtggaggtga gcaatccccc ggg                                             23


<210>  98

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  98
acctacagga gtacctgacg cgg                                             23


<210>  99

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  99
gtgatcacac tccatgtggg agg                                             23


<210>  100

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  100
gctggtgtta tattctgatg ggg                                             23


<210>  101

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  101
cacagtgatc acactccatg tgg                                             23


<210>  102

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  102
cacatggagt gtgatcactg tgg                                             23


<210>  103

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  103
cagagtaggg tccagacgca ggg                                             23


<210>  104

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  104
gcttctgctg ccggttaacg cgg                                             23


<210>  105

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  105
gtggatgacc tggctaacag tgg                                             23


<210>  106

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  106
gggaaagccc aaagtcacac agg                                             23


<210>  107

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  107
aatatgacta gcaacaagtc agg                                             23


<210>  108

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  108
atggagtgtg atcactgtgg agg                                             23


<210>  109

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  109
atcaccgcct tccacgccaa ggg                                             23


<210>  110

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  110
aggagcggaa gacggagttg ggg                                             23


<210>  111

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  111
ccacgccaag ggcaacctac agg                                             23


<210>  112

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  112
caagatgccc atcgtgcaca ggg                                             23


<210>  113

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  113
gatatggcaa ctcccagtgg tgg                                             23


<210>  114

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  114
agcagaagct gagttcaacc tgg                                             23


<210>  115

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  115
cctgtaggtt gcccttggcg tgg                                             23


<210>  116

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  116
gtgagcaatc ccccgggcga ggg                                             23


<210>  117

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  117
acagagtagg gtccagacgc agg                                             23


<210>  118

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  118
gacgcggcat gtcatcagct ggg                                             23


<210>  119

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  119
ggtcatccac agacagagta ggg                                             23


<210>  120

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  120
agtcaagatc tttccctatg agg                                             23


<210>  121

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  121
gaacatactc cagttcctga cgg                                             23


<210>  122

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  122
acgtggagct gatgtcagag cgg                                             23


<210>  123

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  123
ggggaaaggt cgctttgctg agg                                             23


<210>  124

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  124
tctggaccct actctgtctg tgg                                             23


<210>  125

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  125
tgggcagctc cctcgcccgg ggg                                             23


<210>  126

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  126
agctgatgac atgccgcgtc agg                                             23


<210>  127

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  127
gccgcgtcag gtactcctgt agg                                             23


<210>  128

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  128
caagaggcat actcctcata ggg                                             23


<210>  129

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  129
ggtgagcaat cccccgggcg agg                                             23


<210>  130

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  130
ttgctggtgt tatattctga tgg                                             23


<210>  131

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  131
cctatgagga gtatgcctct tgg                                             23


<210>  132

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  132
tgctggtgtt atattctgat ggg                                             23


<210>  133

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  133
cgaggatatt ggagctcttg agg                                             23


<210>  134

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  134
gttgatgttg ttggcacacg tgg                                             23


<210>  135

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  135
tgacgcggca tgtcatcagc tgg                                             23


<210>  136

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  136
ttgagctgga caccctggtg ggg                                             23


<210>  137

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  137
ttcagagcag tttgagacag tgg                                             23


<210>  138

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  138
gcggcatgtc atcagctggg agg                                             23


<210>  139

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  139
aggtcatcca cagacagagt agg                                             23


<210>  140

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  140
gaagatgatg atgacagata tgg                                             23


<210>  141

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  141
tgacctggct aacagtgggc agg                                             23


<210>  142

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  142
tctactgcta ccgcgttaac cgg                                             23


<210>  143

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  143
gtccttctct gtcttccaag agg                                             23


<210>  144

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  144
attgagctgg acaccctggt ggg                                             23


<210>  145

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  145
gtcgttcttc acgaggatat tgg                                             23


<210>  146

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  146
catcagcctc ctgccaccac tgg                                             23


<210>  147

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  147
agttcctgac ggctgaggag cgg                                             23


<210>  148

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  148
actccagttc ctgacggctg agg                                             23


<210>  149

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  149
cttgaggtcc ctgtgcacga tgg                                             23


<210>  150

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  150
ttgaggtccc tgtgcacgat ggg                                             23


<210>  151

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  151
ccgcgtcttg ccggtttccc agg                                             23


<210>  152

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  152
gttgcccttg gcgtggaagg cgg                                             23


<210>  153

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  153
ctttgggctt tccctgcgtc tgg                                             23


<210>  154

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  154
gatcaccgcc ttccacgcca agg                                             23


<210>  155

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  155
tgaagtgttc tgcttcagct tgg                                             23


<210>  156

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  156
ctgtgcacga tgggcatctt ggg                                             23


<210>  157

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  157
ggcatcttgg gcctcccaca tgg                                             23


<210>  158

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  158
ttacctgccc actgttagcc agg                                             23


<210>  159

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  159
atcagcctcc tgccaccact ggg                                             23


<210>  160

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  160
tcgctttgct gaggtctata agg                                             23


<210>  161

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  161
agtcacacag gcagcaggtt agg                                             23


<210>  162

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  162
gaggagcgga agacggagtt ggg                                             23


<210>  163

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  163
tgggcagcag ctctgtgttg tgg                                             23


<210>  164

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  164
tagcaacaag tcaggattgc tgg                                             23


<210>  165

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  165
ccaagatgcc catcgtgcac agg                                             23


<210>  166

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  166
tgtggaggtg agcaatcccc cgg                                             23


<210>  167

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  167
tgcctcttgg aagacagaga agg                                             23


<210>  168

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  168
gcccattgag ctggacaccc tgg                                             23


<210>  169

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  169
ctgagttcaa cctgggaaac cgg                                             23


<210>  170

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  170
tgggaggacc tgcgcaagct ggg                                             23


<210>  171

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  171
gtactcctgt aggttgccct tgg                                             23


<210>  172

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  172
tcttccgctc ctcagccgtc agg                                             23


<210>  173

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  173
ctgggcagct ccctcgcccg ggg                                             23


<210>  174

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  174
cattgagctg gacaccctgg tgg                                             23


<210>  175

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  175
tggcaactcc cagtggtggc agg                                             23


<210>  176

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  176
caactcccag tggtggcagg agg                                             23


<210>  177

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  177
cgagcactgt gccatcatcc tgg                                             23


<210>  178

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  178
gcggtcatct tccaggatga tgg                                             23


<210>  179

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  179
agagctgctg cccattgagc tgg                                             23


<210>  180

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  180
accagggtgt ccagctcaat ggg                                             23


<210>  181

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  181
ctggacaccc tggtggggaa agg                                             23


<210>  182

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  182
caaagcgacc tttccccacc agg                                             23


<210>  183

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  183
gacggctgag gagcggaaga cgg                                             23


<210>  184

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  184
tactctgtct gtggatgacc tgg                                             23


<210>  185

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  185
cggcaagacg cggaagctca tgg                                             23


<210>  186

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  186
cccaaagtca cacaggcagc agg                                             23


<210>  187

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  187
taggttgccc ttggcgtgga agg                                             23


<210>  188

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  188
tgaggagcgg aagacggagt tgg                                             23


<210>  189

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  189
cctgtgcacg atgggcatct tgg                                             23


<210>  190

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  190
gggagctgcc cagcttgcgc agg                                             23


<210>  191

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  191
ttgaactcag cttctgctgc cgg                                             23


<210>  192

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  192
ccaagaggca tactcctcat agg                                             23


<210>  193

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  193
catgagcttc cgcgtcttgc cgg                                             23


<210>  194

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  194
cggagttggg gaaacaatac tgg                                             23


<210>  195

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  195
gctcctcagc cgtcaggaac tgg                                             23


<210>  196

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  196
caccagggtg tccagctcaa tgg                                             23


<210>  197

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  197
ctagtcatat ttcaagtgac agg                                             23


<210>  198

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  198
gctgggcagc tccctcgccc ggg                                             23


<210>  199

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  199
tgtcagagcg gtcatcttcc agg                                             23


<210>  200

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  200
ctgggaggac ctgcgcaagc tgg                                             23


<210>  201

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  201
agctgggcag ctccctcgcc cgg                                             23


<210>  202

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  202
tgtgttgtgg ttgatgttgt tgg                                             23


<210>  203

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  203
cctgctgcct gtgtgacttt ggg                                             23


<210>  204

<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  sgRNA sequence targetting TGFbetaRII gene

<400>  204
acctgctgcc tgtgtgactt tgg                                             23


<210>  205

<211>  1521
<212>  DNA
<213>  artificial

<220>
<223>  DNA encoding CAR CLS MUC1-A

<400>  205
caggttcaac ttcaacaatc aggtgctgaa ttggcgagac ccggcgcttc agtgaaactt     60
tcctgtaagg catccggtta cacttttaca aattatggtc tctcctgggt caagcagcgc    120
accgggcagg gcctcgagtg gattggcgaa aatcaccctg gaagcggcat tatatatcat    180
aacgagaaat tcagaggtaa ggcaacgttg acggcagata agagttcaag taccgcttat    240
gtccaactca gctcattgac ttctgaggac agtgcagttt acttctgcgc acgaagttca    300
ggcacgcggg ggtttgccta ctgggggcag ggaacattgg ttactgtctc agccggagga    360
ggcggtagcg gcggcggagg tagtggaggg ggtggaagtg atgttctgat gactcagacg    420
cctctctctc tgcccgtcag cctgggagat caggcgagca tatcatgcag aagttcccaa    480
agtattgttc attcaaacgg taacacgtat cttgaatggt atttgcagaa accggggcaa    540
agtccaaaac tgctcatata taaagtctca aaccgcttta gcggtgtacc ggataggttt    600
tccgggagcg ggtcagggac agacttcact ctcaagattt cccgagtcga agcagaagat    660
ctcggcgtgt actattgctt ccagggttca cacggccctt ggactttcgg aggaggaact    720
aaactcgaaa ttaagcgagc cggatcagga ggtgggggca gctgcccgta cagtaatcca    780
agtctctgct ccggaggcgg cggctcatgc ccatattcta atccatcact ttgcagcggc    840
gggggtggta gcactaccac accagcacct cgaccaccaa ctccagcacc aaccattgca    900
tcacagccac tgagcctgcg accagaggcc tgtagacctg ctgcaggagg agctgtgcat    960
acccgaggac tggactttgc ctgcgatatc tacatttggg ctcctctggc aggaacttgt   1020
ggcgtgctgc tgctgtccct ggtcatcacc ctgtactgca agcgaggccg gaagaaactg   1080
ctgtatattt tcaaacagcc ctttatgaga cctgtgcaga ctacccagga ggaagacggc   1140
tgcagctgta ggttccccga ggaagaggaa ggcgggtgtg agctgagggt caagtttagc   1200
cgctccgcag atgcccctgc ttaccagcag gggcagaatc agctgtataa cgagctgaat   1260
ctgggacgga gagaggaata cgacgtgctg gataaaaggc gcgggagaga ccccgaaatg   1320
ggaggcaagc cacgacggaa aaacccccag gagggcctgt acaatgaact gcagaaggac   1380
aaaatggcag aggcctatag tgaaatcggg atgaagggag agagaaggcg cggcaaaggg   1440
cacgatggcc tgtaccaggg gctgtctact gccaccaagg acacctatga tgctctgcat   1500
atgcaggcac tgcctccaag g                                             1521


<210>  206

<211>  1503
<212>  DNA
<213>  artificial

<220>
<223>  DNA encoding CAR CLS MUC1-B

<400>  206
gaagtacagc ttcagcaatc tgggggagga ctggttcaac ctgggggatc aatgaaactc     60
tcttgcgtcg cttcagggtt cacattcagc aactattgga tgaactgggt gcgacaaagc    120
cccgaaaagg gtctggaatg ggttgctgag atacgactga agagcaataa ctatgccaca    180
cactacgctg agtccgtcaa ggggagattc acaataagcc gggacgatag taaaagcagt    240
gtatatcttc aaatgaacaa tctgcgagct gaggacactg gaatatatta ctgtactttt    300
ggaaactctt tcgcctactg gggacaagga acgacggtaa cagtttcttc cggaggaggc    360
ggtagcggcg gcggaggtag tggagggggt ggaagtgaca tcgtagtaac gcaggagtca    420
gcgttgacca cctcccccgg tgagactgtg acactgacct gtcggagtag cactggcgca    480
gtcacgacca gcaattacgc gaactgggtt caagaaaaac cggaccatct cttcactgga    540
ctcatagggg ggactaacaa tcgggcaccg ggggtgcctg caaggttttc tggttctctt    600
atcggagata aggcagcact gacaataact ggggcgcaaa ctgaggatga ggctatttac    660
ttttgtgcac tctggtactc caaccactgg gtcttcgggg gtggcacgaa attgaccgta    720
ctcggatcag gaggtggggg cagctgcccg tacagtaatc caagtctctg ctccggaggc    780
ggcggctcat gcccatattc taatccatca ctttgcagcg gcgggggtgg tagcactacc    840
acaccagcac ctcgaccacc aactccagca ccaaccattg catcacagcc actgagcctg    900
cgaccagagg cctgtagacc tgctgcagga ggagctgtgc atacccgagg actggacttt    960
gcctgcgata tctacatttg ggctcctctg gcaggaactt gtggcgtgct gctgctgtcc   1020
ctggtcatca ccctgtactg caagcgaggc cggaagaaac tgctgtatat tttcaaacag   1080
ccctttatga gacctgtgca gactacccag gaggaagacg gctgcagctg taggttcccc   1140
gaggaagagg aaggcgggtg tgagctgagg gtcaagttta gccgctccgc agatgcccct   1200
gcttaccagc aggggcagaa tcagctgtat aacgagctga atctgggacg gagagaggaa   1260
tacgacgtgc tggataaaag gcgcgggaga gaccccgaaa tgggaggcaa gccacgacgg   1320
aaaaaccccc aggagggcct gtacaatgaa ctgcagaagg acaaaatggc agaggcctat   1380
agtgaaatcg ggatgaaggg agagagaagg cgcggcaaag ggcacgatgg cctgtaccag   1440
gggctgtcta ctgccaccaa ggacacctat gatgctctgc atatgcaggc actgcctcca   1500
agg                                                                 1503


<210>  207

<211>  1506
<212>  DNA
<213>  artificial

<220>
<223>  DNA encoding CAR CLS MUC1-C

<400>  207
cagatgcaac tggttcaatc cgaagcggag ttgaagaaac ctggcgcatc tgtcaaagta     60
tcctgcaaag ctagtggtta cagcttcacg ggtcactata tgcactgggt cagacaagcg    120
ccaggccagg gactggaatg gatggggtgg atagacccgg tgaccggagg aactaagtat    180
gctcagaact tccaaggatg ggtcacaatg acaagggata cgtccatccg aaccgcctac    240
cttgaactca gccgactccg gtctgacgat actgcaatgt actattgcgc gagagaagta    300
accggggaca gggggcaatt tgacaaatgg ggacagggga ccttggtcac tgtggccagc    360
ggaggaggcg gtagcggcgg cggaggtagt ggagggggtg gaagtcagag tgtgctcacc    420
cagcctccat ccgtgtctgt ggcacctggt aaaacggcac gaattacttg tggggggaac    480
aatattggat ctaaatctgt ccactggtat cagcagaaac cggggcaagc tccggcgctt    540
gtcatatact atggatctaa ccgaccgagc gggattccag aacggtttag cggctctaat    600
agcggtaata cagcaactct tacgatttca agggtagaag cgggcgatga ggcagactac    660
tactgccagg tatgggatag tagttctgat tgggtcttcg gcggggggac aaaacttacg    720
gttctcggat caggaggtgg gggcagctgc ccgtacagta atccaagtct ctgctccgga    780
ggcggcggct catgcccata ttctaatcca tcactttgca gcggcggggg tggtagcact    840
accacaccag cacctcgacc accaactcca gcaccaacca ttgcatcaca gccactgagc    900
ctgcgaccag aggcctgtag acctgctgca ggaggagctg tgcatacccg aggactggac    960
tttgcctgcg atatctacat ttgggctcct ctggcaggaa cttgtggcgt gctgctgctg   1020
tccctggtca tcaccctgta ctgcaagcga ggccggaaga aactgctgta tattttcaaa   1080
cagcccttta tgagacctgt gcagactacc caggaggaag acggctgcag ctgtaggttc   1140
cccgaggaag aggaaggcgg gtgtgagctg agggtcaagt ttagccgctc cgcagatgcc   1200
cctgcttacc agcaggggca gaatcagctg tataacgagc tgaatctggg acggagagag   1260
gaatacgacg tgctggataa aaggcgcggg agagaccccg aaatgggagg caagccacga   1320
cggaaaaacc cccaggaggg cctgtacaat gaactgcaga aggacaaaat ggcagaggcc   1380
tatagtgaaa tcgggatgaa gggagagaga aggcgcggca aagggcacga tggcctgtac   1440
caggggctgt ctactgccac caaggacacc tatgatgctc tgcatatgca ggcactgcct   1500
ccaagg                                                              1506


<210>  208

<211>  1512
<212>  DNA
<213>  artificial

<220>
<223>  DNA encoding CAR CLS MUC1-D

<400>  208
caggtacagc tccagcaatc cgacgcggaa ttggttaaac cggggagctc cgtaaaaatt     60
tcatgcaaag cgtctggcta taccttcaca gaccacgcta tacattgggt gaagcagaaa    120
ccagaacaag gactcgaatg gatcggtcac ttctcaccgg gtaatacgga cattaaatac    180
aacgacaaat tcaagggaaa ggcgacactc acggtagata gatcaagttc cacagcttat    240
atgcaactga atagcctcac ttctgaggat tccgcagtct atttttgcaa aacaagcacc    300
tttttctttg actattgggg ccaaggcact actttgacag tgtcctctgg aggaggcggt    360
agcggcggcg gaggtagtgg agggggtgga agtgacatcg taatgactca atccccgtca    420
agtcttaccg tgacggcagg cgaaaaggta accatgatct gcaagtcatc tcaatccctg    480
cttaacagtg gtgaccaaaa gaattacctg acatggtacc aacaaaagcc cggccagcca    540
ccaaaactcc tcatattttg ggcgtcaacc agagagagcg gtgtacctga tagatttacc    600
ggaagcggga gcggaacaga ttttacactt actatttcct ctgtccaggc cgaagacctc    660
gcggtgtact actgtcagaa tgactattct tacccgctga ccttcggggc cggaacgaag    720
ctggagttga aaggatcagg aggtgggggc agctgcccgt acagtaatcc aagtctctgc    780
tccggaggcg gcggctcatg cccatattct aatccatcac tttgcagcgg cgggggtggt    840
agcactacca caccagcacc tcgaccacca actccagcac caaccattgc atcacagcca    900
ctgagcctgc gaccagaggc ctgtagacct gctgcaggag gagctgtgca tacccgagga    960
ctggactttg cctgcgatat ctacatttgg gctcctctgg caggaacttg tggcgtgctg   1020
ctgctgtccc tggtcatcac cctgtactgc aagcgaggcc ggaagaaact gctgtatatt   1080
ttcaaacagc cctttatgag acctgtgcag actacccagg aggaagacgg ctgcagctgt   1140
aggttccccg aggaagagga aggcgggtgt gagctgaggg tcaagtttag ccgctccgca   1200
gatgcccctg cttaccagca ggggcagaat cagctgtata acgagctgaa tctgggacgg   1260
agagaggaat acgacgtgct ggataaaagg cgcgggagag accccgaaat gggaggcaag   1320
ccacgacgga aaaaccccca ggagggcctg tacaatgaac tgcagaagga caaaatggca   1380
gaggcctata gtgaaatcgg gatgaaggga gagagaaggc gcggcaaagg gcacgatggc   1440
ctgtaccagg ggctgtctac tgccaccaag gacacctatg atgctctgca tatgcaggca   1500
ctgcctccaa gg                                                       1512


<210>  209

<211>  291
<212>  PRT
<213>  homo sapiens

<220>
<223>  MESOTHELIN target antigen region

<400>  209
Glu Val Glu Lys Thr Ala Cys Pro Ser Gly Lys Lys Ala Arg Glu Ile 
1               5                   10                  15      


Asp Glu Ser Leu Ile Phe Tyr Lys Lys Trp Glu Leu Glu Ala Cys Val 
            20                  25                  30          


Asp Ala Ala Leu Leu Ala Thr Gln Met Asp Arg Val Asn Ala Ile Pro 
        35                  40                  45              


Phe Thr Tyr Glu Gln Leu Asp Val Leu Lys His Lys Leu Asp Glu Leu 
    50                  55                  60                  


Tyr Pro Gln Gly Tyr Pro Glu Ser Val Ile Gln His Leu Gly Tyr Leu 
65                  70                  75                  80  


Phe Leu Lys Met Ser Pro Glu Asp Ile Arg Lys Trp Asn Val Thr Ser 
                85                  90                  95      


Leu Glu Thr Leu Lys Ala Leu Leu Glu Val Asn Lys Gly His Glu Met 
            100                 105                 110         


Ser Pro Gln Ala Pro Arg Arg Pro Leu Pro Gln Val Ala Thr Leu Ile 
        115                 120                 125             


Asp Arg Phe Val Lys Gly Arg Gly Gln Leu Asp Lys Asp Thr Leu Asp 
    130                 135                 140                 


Thr Leu Thr Ala Phe Tyr Pro Gly Tyr Leu Cys Ser Leu Ser Pro Glu 
145                 150                 155                 160 


Glu Leu Ser Ser Val Pro Pro Ser Ser Ile Trp Ala Val Arg Pro Gln 
                165                 170                 175     


Asp Leu Asp Thr Cys Asp Pro Arg Gln Leu Asp Val Leu Tyr Pro Lys 
            180                 185                 190         


Ala Arg Leu Ala Phe Gln Asn Met Asn Gly Ser Glu Tyr Phe Val Lys 
        195                 200                 205             


Ile Gln Ser Phe Leu Gly Gly Ala Pro Thr Glu Asp Leu Lys Ala Leu 
    210                 215                 220                 


Ser Gln Gln Asn Val Ser Met Asp Leu Ala Thr Phe Met Lys Leu Arg 
225                 230                 235                 240 


Thr Asp Ala Val Leu Pro Leu Thr Val Ala Glu Val Gln Lys Leu Leu 
                245                 250                 255     


Gly Pro His Val Glu Gly Leu Lys Ala Glu Glu Arg His Arg Pro Val 
            260                 265                 270         


Arg Asp Trp Ile Leu Arg Gln Arg Gln Asp Asp Leu Asp Thr Leu Gly 
        275                 280                 285             


Leu Gly Leu 
    290     


<210>  210

<211>  116
<212>  PRT
<213>  artificial

<220>
<223>  MESO1 heavy chain variable region

<400>  210
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Ser Tyr 
            20                  25                  30          


Tyr Trp Ser Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys 
    50                  55                  60                  


Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe Ser Leu 
65                  70                  75                  80  


Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Val Asp Tyr Lys Ala Phe Asp Ile Trp Gly Gln Gly Thr Met Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  211

<211>  107
<212>  PRT
<213>  artificial

<220>
<223>  MESO1 light chain variable region

<400>  211
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Asp 
            20                  25                  30          


Leu His Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln His Tyr Ser Tyr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  212

<211>  121
<212>  PRT
<213>  artificial

<220>
<223>  MESO2 heavy chain variable region

<400>  212
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Val Ile Met Pro Ser Asp Ser Tyr Thr Arg Tyr Ser Pro Ser Phe 
    50                  55                  60                  


Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Gly His Gly Met Tyr Gly Gly Ala Leu Asp Val Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120     


<210>  213

<211>  111
<212>  PRT
<213>  artificial

<220>
<223>  MESO2 light chain variable region

<400>  213
Asp Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Arg Ser Ser 
            20                  25                  30          


Arg Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Ser Lys Arg Ala Thr Gly Val Pro Ala Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Ser His Asp Pro 
                85                  90                  95      


Ser Gly Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr 
            100                 105                 110     


<210>  214

<211>  127
<212>  PRT
<213>  artificial

<220>
<223>  P4 heavy chain variable region

<400>  214
Gln Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Thr Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly Asp Ser Val Ser Ser Asn 
            20                  25                  30          


Ser Ala Thr Trp Asn Trp Ile Arg Gln Ser Pro Ser Arg Gly Leu Glu 
        35                  40                  45              


Trp Leu Gly Arg Thr Tyr Tyr Arg Ser Lys Trp Tyr Asn Asp Tyr Ala 
    50                  55                  60                  


Val Ser Val Lys Ser Arg Met Ser Ile Asn Pro Asp Thr Ser Lys Asn 
65                  70                  75                  80  


Gln Phe Ser Leu Gln Leu Asn Ser Val Thr Pro Glu Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Gly Met Met Thr Tyr Tyr Tyr Gly Met Asp Val 
            100                 105                 110         


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Ile Leu Gly 
        115                 120                 125         


<210>  215

<211>  116
<212>  PRT
<213>  artificial

<220>
<223>  P4 light chain variable region

<400>  215
Gln Pro Val Leu Thr Gln Ser Ser Ser Leu Ser Ala Ser Pro Gly Ala 
1               5                   10                  15      


Ser Ala Ser Leu Thr Cys Thr Leu Arg Ser Gly Ile Asn Val Gly Pro 
            20                  25                  30          


Tyr Arg Ile Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Pro Pro Gln Tyr 
        35                  40                  45              


Leu Leu Asn Tyr Lys Ser Asp Ser Asp Lys Gln Gln Gly Ser Gly Val 
    50                  55                  60                  


Pro Ser Arg Phe Ser Gly Ser Lys Asp Ala Ser Ala Asn Ala Gly Val 
65                  70                  75                  80  


Leu Leu Ile Ser Gly Leu Arg Ser Glu Asp Glu Ala Asp Tyr Tyr Cys 
                85                  90                  95      


Met Ile Trp His Ser Ser Ala Ala Val Phe Gly Gly Gly Thr Gln Leu 
            100                 105                 110         


Thr Val Leu Ser 
        115     


<210>  216

<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  CDRH1-Meso1

<400>  216
Ser Tyr Tyr Trp Ser 
1               5   


<210>  217

<211>  16
<212>  PRT
<213>  artificial

<220>
<223>  CDRH2- meso1

<400>  217
Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys Ser 
1               5                   10                  15      






<210>  218

<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  CDRH3-meso1

<400>  218
Val Asp Tyr Lys Ala Phe Asp Ile 
1               5               


<210>  219

<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  CDRL1- meso1

<400>  219
Arg Ala Ser Gln Gly Ile Arg Asn Asp Leu His 
1               5                   10      


<210>  220

<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  CDRL2- meso1

<400>  220
Ala Ala Ser Ser Leu Gln Ser 
1               5           


<210>  221

<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  CDRL3- meso1

<400>  221
Leu Gln His Tyr Ser Tyr Pro Trp Thr 
1               5                   


<210>  222

<211>  482
<212>  PRT
<213>  artificial

<220>
<223>  MESO1 CAR full sequence

<400>  222
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu 
            20                  25                  30          


Val Lys Pro Ser Glu Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly 
        35                  40                  45              


Ser Ile Ser Ser Tyr Tyr Trp Ser Trp Ile Arg Gln Pro Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Tyr Ser Gly Ser Thr Asn Tyr 
65                  70                  75                  80  


Asn Pro Ser Leu Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys 
                85                  90                  95      


Asn Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Val Asp Tyr Lys Ala Phe Asp Ile Trp Gly 
        115                 120                 125             


Gln Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro 
145                 150                 155                 160 


Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg 
                165                 170                 175     


Ala Ser Gln Gly Ile Arg Asn Asp Leu His Trp Tyr Gln Gln Lys Pro 
            180                 185                 190         


Gly Lys Ala Pro Lys Arg Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser 
        195                 200                 205             


Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr 
    210                 215                 220                 


Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys 
225                 230                 235                 240 


Leu Gln His Tyr Ser Tyr Pro Trp Thr Phe Gly Gln Gly Thr Lys Val 
                245                 250                 255     


Glu Ile Lys Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
            260                 265                 270         


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
        275                 280                 285             


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
    290                 295                 300                 


Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
305                 310                 315                 320 


Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu 
                325                 330                 335     


Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
            340                 345                 350         


Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
        355                 360                 365             


Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
    370                 375                 380                 


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
385                 390                 395                 400 


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
                405                 410                 415     


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
            420                 425                 430         


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
        435                 440                 445             


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
    450                 455                 460                 


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
465                 470                 475                 480 


Pro Arg 
        

<210>  223

<211>  925
<212>  PRT
<213>  artificial

<220>
<223>  TGFBR2 TALEN Left

<400>  223
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Ile Ala Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Ala Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
545                 550                 555                 560 


Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
            580                 585                 590         


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln 
        595                 600                 605             


Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Asp His 
    610                 615                 620                 


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly 
625                 630                 635                 640 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                645                 650                 655     


Asp His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
            660                 665                 670         


Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro 
        675                 680                 685             


Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala 
    690                 695                 700                 


Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Arg Arg Gly Leu Gly 
705                 710                 715                 720 


Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys 
                725                 730                 735     


Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile 
            740                 745                 750         


Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu 
        755                 760                 765             


Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys 
    770                 775                 780                 


His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly 
785                 790                 795                 800 


Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly 
                805                 810                 815     


Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val 
            820                 825                 830         


Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp 
        835                 840                 845             


Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser 
    850                 855                 860                 


Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His 
865                 870                 875                 880 


Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile 
                885                 890                 895     


Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg 
            900                 905                 910         


Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
        915                 920                 925 


<210>  224

<211>  925
<212>  PRT
<213>  artificial

<220>
<223>  TGFBR2 TALEN Right

<400>  224
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Ile Ala Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Ala Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
545                 550                 555                 560 


Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
            580                 585                 590         


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln 
        595                 600                 605             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
    610                 615                 620                 


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly 
625                 630                 635                 640 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                645                 650                 655     


Asp His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
            660                 665                 670         


Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro 
        675                 680                 685             


Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala 
    690                 695                 700                 


Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Arg Arg Gly Leu Gly 
705                 710                 715                 720 


Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys 
                725                 730                 735     


Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile 
            740                 745                 750         


Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu 
        755                 760                 765             


Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys 
    770                 775                 780                 


His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly 
785                 790                 795                 800 


Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly 
                805                 810                 815     


Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val 
            820                 825                 830         


Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp 
        835                 840                 845             


Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser 
    850                 855                 860                 


Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His 
865                 870                 875                 880 


Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile 
                885                 890                 895     


Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg 
            900                 905                 910         


Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
        915                 920                 925 


<210>  225

<211>  59
<212>  DNA
<213>  homo sapiens

<220>
<223>  TRAC target sequence

<400>  225
tgatcctctt gtcccacaga tatccagaac cctgaccctg ccgtgtacca gctgagaga      59


<210>  226

<211>  49
<212>  DNA
<213>  homo sapiens

<220>
<223>  PD1 target sequence

<400>  226
tacctctgtg gggccatctc cctggccccc aaggcgcaga tcaaagaga                 49


<210>  227

<211>  47
<212>  DNA
<213>  homo sapiens

<220>
<223>  B2M target sequence

<400>  227
ttagctgtgc tcgcgctact ctctctttct ggcctggagg ctatcca                   47

<210>  228

<211>  118
<212>  PRT
<213>  artificial

<220>
<223>  MUC1-A VH humanized H1

<400>  228
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Leu Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Asn His Pro Gly Ser Gly Ile Ile Tyr His Asn Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Ser Gly Thr Arg Gly Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  229

<211>  118
<212>  PRT
<213>  artificial

<220>
<223>  MUC1-A VH humanized H2

<400>  229
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Leu Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Asn His Pro Gly Ser Gly Ile Ile Tyr His Asn Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Ala Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Ser Gly Thr Arg Gly Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  230

<211>  118
<212>  PRT
<213>  artificial

<220>
<223>  MUC1-A VH humanized H3

<400>  230
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Leu Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Asn His Pro Gly Ser Gly Ile Ile Tyr His Asn Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Arg Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Ser Gly Thr Arg Gly Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  231

<211>  118
<212>  PRT
<213>  artificial

<220>
<223>  MUC1-A VH humanized H4

<400>  231
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Leu Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Asn His Pro Gly Ser Gly Ile Ile Tyr His Asn Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Ala Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Ser Gly Thr Arg Gly Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  232

<211>  118
<212>  PRT
<213>  artificial

<220>
<223>  MUC1-A VH humanized H5

<400>  232
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Leu Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Asn His Pro Gly Ser Gly Ile Ile Tyr His Asn Glu Lys Phe 
    50                  55                  60                  


Arg Gly Arg Val Thr Met Thr Ala Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Ser Gly Thr Arg Gly Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  233

<211>  118
<212>  PRT
<213>  artificial

<220>
<223>  MUC1-A VH humanized H6

<400>  233
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Leu Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Asn His Pro Gly Ser Gly Ile Ile Tyr His Asn Glu Lys Phe 
    50                  55                  60                  


Arg Gly Arg Val Thr Met Thr Arg Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Ser Gly Thr Arg Gly Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  234

<211>  118
<212>  PRT
<213>  artificial

<220>
<223>  MUC1-A VH humanized H7

<400>  234
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Leu Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Asn His Pro Gly Ser Gly Ile Ile Tyr His Asn Glu Lys Phe 
    50                  55                  60                  


Arg Gly Arg Val Thr Met Thr Ala Asp Lys Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Ser Gly Thr Arg Gly Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  235

<211>  114
<212>  PRT
<213>  artificial

<220>
<223>  MUC1-A VL humanized L1

<400>  235
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Glu Trp Phe Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Arg Arg Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      


Ser His Gly Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


Arg Ala 
        


<210>  236

<211>  114
<212>  PRT
<213>  artificial

<220>
<223>  MUC1-A VL humanized L2

<400>  236
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Arg Arg Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      


Ser His Gly Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


Arg Ala 
        


<210>  237

<211>  114
<212>  PRT
<213>  artificial

<220>
<223>  MUC1-A VL humanized L3

<400>  237
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Glu Trp Phe Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      


Ser His Gly Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


Arg Ala 
        


<210>  238

<211>  114
<212>  PRT
<213>  artificial

<220>
<223>  MUC1-A VL humanized L4

<400>  238
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      


Ser His Gly Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


Arg Ala 
        


<210>  239

<211>  114
<212>  PRT
<213>  artificial

<220>
<223>  MUC1-A VL humanized L5

<400>  239
Asp Val Leu Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      


Ser His Gly Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


Arg Ala 
        



























