                         SEQUENCE LISTING

<110>  The United States of America as Represented by the 
       Secretary, Department of Health and Human Services
 
<120>  PREFUSION-STABILIZED LASSA VIRUS GLYCOPROTEIN COMPLEX AND ITS USE

<130>  4239-108204-02

<150>  63/329,886
<151>  2022-04-12

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  491
<212>  PRT
<213>  Lassa virus

<400>  1

Met Gly Gln Ile Val Thr Phe Phe Gln Glu Val Pro His Val Ile Glu 
1               5                   10                  15      


Glu Val Met Asn Ile Val Leu Ile Ala Leu Ser Val Leu Ala Val Leu 
            20                  25                  30          


Lys Gly Leu Tyr Asn Phe Ala Thr Cys Gly Leu Val Gly Leu Val Thr 
        35                  40                  45              


Phe Leu Leu Leu Cys Gly Arg Ser Cys Thr Thr Ser Leu Tyr Lys Gly 
    50                  55                  60                  


Val Tyr Glu Leu Gln Thr Leu Glu Leu Asn Met Glu Thr Leu Asn Met 
65                  70                  75                  80  


Thr Met Pro Leu Ser Cys Thr Lys Asn Asn Ser His His Tyr Ile Met 
                85                  90                  95      


Val Gly Asn Glu Thr Gly Leu Glu Leu Thr Leu Thr Asn Thr Ser Ile 
            100                 105                 110         


Ile Asn His Lys Phe Cys Asn Leu Ser Asp Ala His Lys Lys Asn Leu 
        115                 120                 125             


Tyr Asp His Ala Leu Met Ser Ile Ile Ser Thr Phe His Leu Ser Ile 
    130                 135                 140                 


Pro Asn Phe Asn Gln Tyr Glu Ala Met Ser Cys Asp Phe Asn Gly Gly 
145                 150                 155                 160 


Lys Ile Ser Val Gln Tyr Asn Leu Ser His Ser Tyr Ala Gly Asp Ala 
                165                 170                 175     


Ala Asn His Cys Gly Thr Val Ala Asn Gly Val Leu Gln Thr Phe Met 
            180                 185                 190         


Arg Met Ala Trp Gly Gly Ser Tyr Ile Ala Leu Asp Ser Gly Arg Gly 
        195                 200                 205             


Asn Trp Asp Cys Ile Met Thr Ser Tyr Gln Tyr Leu Ile Ile Gln Asn 
    210                 215                 220                 


Thr Thr Trp Glu Asp His Cys Gln Phe Ser Arg Pro Ser Pro Ile Gly 
225                 230                 235                 240 


Tyr Leu Gly Leu Leu Ser Gln Arg Thr Arg Asp Ile Tyr Ile Ser Arg 
                245                 250                 255     


Arg Leu Leu Gly Thr Phe Thr Trp Thr Leu Ser Asp Ser Glu Gly Lys 
            260                 265                 270         


Asp Thr Pro Gly Gly Tyr Cys Leu Thr Arg Trp Met Leu Ile Glu Ala 
        275                 280                 285             


Glu Leu Lys Cys Phe Gly Asn Thr Ala Val Ala Lys Cys Asn Glu Lys 
    290                 295                 300                 


His Asp Glu Glu Phe Cys Asp Met Leu Arg Leu Phe Asp Phe Asn Lys 
305                 310                 315                 320 


Gln Ala Ile Gln Arg Leu Lys Ala Glu Ala Gln Met Ser Ile Gln Leu 
                325                 330                 335     


Ile Asn Lys Ala Val Asn Ala Leu Ile Asn Asp Gln Leu Ile Met Lys 
            340                 345                 350         


Asn His Leu Arg Asp Ile Met Gly Ile Pro Tyr Cys Asn Tyr Ser Lys 
        355                 360                 365             


Tyr Trp Tyr Leu Asn His Thr Thr Thr Gly Arg Thr Ser Leu Pro Lys 
    370                 375                 380                 


Cys Trp Leu Val Ser Asn Gly Ser Tyr Leu Asn Glu Thr His Phe Ser 
385                 390                 395                 400 


Asp Asp Ile Glu Gln Gln Ala Asp Asn Met Ile Thr Glu Met Leu Gln 
                405                 410                 415     


Lys Glu Tyr Met Glu Arg Gln Gly Lys Thr Pro Leu Gly Leu Val Asp 
            420                 425                 430         


Leu Phe Val Phe Ser Thr Ser Phe Tyr Leu Ile Ser Ile Phe Leu His 
        435                 440                 445             


Leu Val Lys Ile Pro Thr His Arg His Ile Val Gly Lys Ser Cys Pro 
    450                 455                 460                 


Lys Pro His Arg Leu Asn His Met Gly Ile Cys Ser Cys Gly Leu Tyr 
465                 470                 475                 480 


Lys Gln Pro Gly Val Pro Val Lys Trp Lys Arg 
                485                 490     


<210>  2
<211>  468
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Engineered LASV GPC protein

<400>  2

Met Gly Gln Ile Val Thr Phe Phe Gln Glu Val Pro His Val Ile Glu 
1               5                   10                  15      


Glu Val Met Asn Ile Val Leu Ile Ala Leu Ser Val Leu Ala Val Leu 
            20                  25                  30          


Lys Gly Leu Tyr Asn Phe Ala Thr Cys Gly Leu Val Gly Leu Val Thr 
        35                  40                  45              


Phe Leu Leu Leu Cys Gly Arg Ser Cys Thr Thr Ser Leu Tyr Lys Gly 
    50                  55                  60                  


Val Tyr Glu Leu Gln Thr Leu Glu Leu Asn Met Glu Thr Leu Asn Met 
65                  70                  75                  80  


Thr Met Pro Leu Ser Cys Thr Lys Asn Asn Ser His His Tyr Ile Met 
                85                  90                  95      


Val Gly Asn Glu Thr Gly Leu Glu Leu Thr Leu Thr Asn Thr Ser Ile 
            100                 105                 110         


Ile Asn His Lys Phe Cys Asn Leu Ser Asp Ala His Lys Lys Asn Leu 
        115                 120                 125             


Tyr Asp His Ala Leu Met Ser Ile Ile Ser Thr Phe His Leu Ser Ile 
    130                 135                 140                 


Pro Asn Phe Asn Gln Tyr Glu Ala Met Ser Cys Asp Phe Asn Gly Gly 
145                 150                 155                 160 


Lys Ile Ser Val Gln Tyr Asn Leu Ser His Ser Tyr Ala Gly Asp Ala 
                165                 170                 175     


Ala Asn His Cys Gly Thr Val Ala Asn Gly Val Leu Gln Thr Phe Met 
            180                 185                 190         


Arg Met Ala Trp Gly Gly Ser Tyr Ile Ala Leu Asp Ser Gly Gly Cys 
        195                 200                 205             


Gly Asn Trp Asp Cys Ile Met Thr Ser Tyr Gln Tyr Leu Ile Ile Gln 
    210                 215                 220                 


Asn Thr Thr Trp Glu Asp His Cys Gln Phe Ser Arg Pro Ser Pro Ile 
225                 230                 235                 240 


Gly Tyr Leu Gly Leu Leu Ser Gln Arg Thr Arg Asp Ile Tyr Ile Ser 
                245                 250                 255     


Arg Arg Arg Arg Gly Thr Phe Thr Trp Thr Leu Ser Asp Ser Glu Gly 
            260                 265                 270         


Lys Asp Thr Pro Gly Gly Tyr Cys Leu Thr Arg Trp Met Leu Ile Glu 
        275                 280                 285             


Ala Glu Leu Lys Cys Phe Gly Asn Thr Ala Val Ala Lys Cys Asn Glu 
    290                 295                 300                 


Lys His Asp Glu Glu Phe Cys Asp Met Leu Arg Leu Phe Asp Phe Asn 
305                 310                 315                 320 


Lys Gln Ala Ile Gln Arg Cys Lys Ala Pro Ala Gln Met Ser Ile Gln 
                325                 330                 335     


Leu Ile Asn Lys Ala Val Asn Ala Leu Ile Asn Asp Gln Leu Ile Met 
            340                 345                 350         


Lys Asn His Leu Arg Asp Ile Met Gly Ile Pro Tyr Cys Asn Tyr Ser 
        355                 360                 365             


Lys Tyr Trp Tyr Leu Asn His Thr Thr Thr Gly Arg Thr Ser Leu Pro 
    370                 375                 380                 


Lys Cys Trp Leu Val Ser Asn Gly Ser Tyr Leu Asn Glu Thr His Phe 
385                 390                 395                 400 


Ser Asp Asp Ile Glu Gln Gln Ala Asp Asn Met Ile Thr Glu Met Leu 
                405                 410                 415     


Gln Lys Glu Gly Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln 
            420                 425                 430         


Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
        435                 440                 445             


Gly Gly Gly Ser Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro 
    450                 455                 460                 


Thr Lys Gly Gly 
465             


<210>  3
<211>  492
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Engineered LASV GPC protein

<400>  3

Met Gly Gln Ile Val Thr Phe Phe Gln Glu Val Pro His Val Ile Glu 
1               5                   10                  15      


Glu Val Met Asn Ile Val Leu Ile Ala Leu Ser Val Leu Ala Val Leu 
            20                  25                  30          


Lys Gly Leu Tyr Asn Phe Ala Thr Cys Gly Leu Val Gly Leu Val Thr 
        35                  40                  45              


Phe Leu Leu Leu Cys Gly Arg Ser Cys Thr Thr Ser Leu Tyr Lys Gly 
    50                  55                  60                  


Val Tyr Glu Leu Gln Thr Leu Glu Leu Asn Met Glu Thr Leu Asn Met 
65                  70                  75                  80  


Thr Met Pro Leu Ser Cys Thr Lys Asn Asn Ser His His Tyr Ile Met 
                85                  90                  95      


Val Gly Asn Glu Thr Gly Leu Glu Leu Thr Leu Thr Asn Thr Ser Ile 
            100                 105                 110         


Ile Asn His Lys Phe Cys Asn Leu Ser Asp Ala His Lys Lys Asn Leu 
        115                 120                 125             


Tyr Asp His Ala Leu Met Ser Ile Ile Ser Thr Phe His Leu Ser Ile 
    130                 135                 140                 


Pro Asn Phe Asn Gln Tyr Glu Ala Met Ser Cys Asp Phe Asn Gly Gly 
145                 150                 155                 160 


Lys Ile Ser Val Gln Tyr Asn Leu Ser His Ser Tyr Ala Gly Asp Ala 
                165                 170                 175     


Ala Asn His Cys Gly Thr Val Ala Asn Gly Val Leu Gln Thr Phe Met 
            180                 185                 190         


Arg Met Ala Trp Gly Gly Ser Tyr Ile Ala Leu Asp Ser Gly Gly Cys 
        195                 200                 205             


Gly Asn Trp Asp Cys Ile Met Thr Ser Tyr Gln Tyr Leu Ile Ile Gln 
    210                 215                 220                 


Asn Thr Thr Trp Glu Asp His Cys Gln Phe Ser Arg Pro Ser Pro Ile 
225                 230                 235                 240 


Gly Tyr Leu Gly Leu Leu Ser Gln Arg Thr Arg Asp Ile Tyr Ile Ser 
                245                 250                 255     


Arg Arg Arg Arg Gly Thr Phe Thr Trp Thr Leu Ser Asp Ser Glu Gly 
            260                 265                 270         


Lys Asp Thr Pro Gly Gly Tyr Cys Leu Thr Arg Trp Met Leu Ile Glu 
        275                 280                 285             


Ala Glu Leu Lys Cys Phe Gly Asn Thr Ala Val Ala Lys Cys Asn Glu 
    290                 295                 300                 


Lys His Asp Glu Glu Phe Cys Asp Met Leu Arg Leu Phe Asp Phe Asn 
305                 310                 315                 320 


Lys Gln Ala Ile Gln Arg Cys Lys Ala Glu Ala Gln Met Ser Ile Gln 
                325                 330                 335     


Leu Ile Asn Lys Ala Val Asn Ala Leu Ile Asn Asp Gln Leu Ile Met 
            340                 345                 350         


Lys Asn His Leu Arg Asp Ile Met Gly Ile Pro Tyr Cys Asn Tyr Ser 
        355                 360                 365             


Lys Tyr Trp Tyr Leu Asn His Thr Thr Thr Gly Arg Thr Ser Leu Pro 
    370                 375                 380                 


Lys Cys Trp Leu Val Ser Asn Gly Ser Tyr Leu Asn Glu Thr His Phe 
385                 390                 395                 400 


Ser Asp Asp Ile Glu Gln Gln Ala Asp Asn Met Ile Thr Glu Met Leu 
                405                 410                 415     


Gln Lys Glu Tyr Met Glu Arg Gln Gly Lys Thr Pro Leu Gly Leu Val 
            420                 425                 430         


Asp Leu Phe Val Phe Ser Thr Ser Phe Tyr Leu Ile Ser Ile Phe Leu 
        435                 440                 445             


His Leu Val Lys Ile Pro Thr His Arg His Ile Val Gly Lys Ser Cys 
    450                 455                 460                 


Pro Lys Pro His Arg Leu Asn His Met Gly Ile Cys Ser Cys Gly Leu 
465                 470                 475                 480 


Tyr Lys Gln Pro Gly Val Pro Val Lys Trp Lys Arg 
                485                 490         


<210>  4
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T4 Fibritin trimerization domain

<400>  4

Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys 
1               5                   10                  15      


Asp Gly Glu Trp Val Leu Leu Ser Thr Phe 
            20                  25      


<210>  5
<211>  338
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Engineered LASV GPC protein

<400>  5

Pro Tyr Gly Trp Glu Tyr Ala Ala His Pro Leu Cys Glu Val Glu Val 
1               5                   10                  15      


Leu Ser Asp Glu Asn Glu Val Val Lys Trp Gly Leu Arg Lys Ser Leu 
            20                  25                  30          


Pro Leu Ile Glu Leu Arg Ala Thr Phe Thr Leu Leu Trp Glu Leu Asp 
        35                  40                  45              


Asn Leu Glu Cys Gly Lys Pro Asn Val Asp Leu Ser Ser Leu Glu Glu 
    50                  55                  60                  


Thr Val Arg Lys Val Ala Glu Phe Glu Asp Glu Val Ile Phe Arg Gly 
65                  70                  75                  80  


Cys Glu Lys Ser Gly Val Lys Gly Leu Leu Ser Phe Glu Glu Arg Lys 
                85                  90                  95      


Ile Glu Cys Gly Ser Thr Pro Lys Asp Leu Leu Glu Ala Ile Val Arg 
            100                 105                 110         


Ala Leu Ser Ile Phe Ser Lys Asp Gly Ile Glu Gly Pro Tyr Thr Leu 
        115                 120                 125             


Val Ile Asn Thr Asp Arg Trp Ile Asn Phe Leu Lys Glu Glu Ala Gly 
    130                 135                 140                 


His Tyr Pro Leu Glu Lys Arg Val Glu Glu Cys Leu Arg Gly Gly Lys 
145                 150                 155                 160 


Ile Ile Thr Thr Pro Arg Ile Glu Asp Ala Leu Val Val Ser Glu Arg 
                165                 170                 175     


Gly Gly Asp Phe Lys Leu Ile Leu Gly Gln Asp Leu Ser Ile Gly Tyr 
            180                 185                 190         


Glu Asp Arg Glu Lys Asp Ala Val Arg Leu Phe Ile Thr Glu Thr Phe 
        195                 200                 205             


Thr Met Leu Leu Lys Phe Gly Ser Gly Ser Gly Ser Val Thr Thr Leu 
    210                 215                 220                 


Ser Gly Leu Ser Gly Glu Gln Gly Pro Ser Gly Asp Met Thr Thr Glu 
225                 230                 235                 240 


Glu Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly 
                245                 250                 255     


Arg Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys 
            260                 265                 270         


Thr Ile Ser Thr Trp Ile Ser Asp Gly His Val Lys Asp Phe Tyr Leu 
        275                 280                 285             


Tyr Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr 
    290                 295                 300                 


Glu Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val 
305                 310                 315                 320 


Thr Val Asn Gly Glu Ala Thr Lys Gly Asp Ala His Thr Gly Ser Ser 
                325                 330                 335     


Gly Ser 
        


<210>  6
<211>  394
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Engineered LASV GPC protein

<400>  6

Met Glu Phe Leu Lys Arg Ser Phe Ala Pro Leu Thr Glu Lys Gln Trp 
1               5                   10                  15      


Gln Glu Ile Asp Asn Arg Ala Arg Glu Ile Phe Lys Thr Gln Leu Tyr 
            20                  25                  30          


Gly Arg Lys Phe Val Asp Val Glu Gly Gly Gly Gly Gly His His His 
        35                  40                  45              


His His His Gly Gly Gly Gly Gly Pro Tyr Gly Trp Glu Tyr Ala Ala 
    50                  55                  60                  


His Pro Leu Cys Glu Val Glu Val Leu Ser Asp Glu Asn Glu Val Val 
65                  70                  75                  80  


Lys Trp Gly Leu Arg Lys Ser Leu Pro Leu Ile Glu Leu Arg Ala Thr 
                85                  90                  95      


Phe Thr Leu Leu Trp Glu Leu Asp Asn Leu Glu Cys Gly Lys Pro Asn 
            100                 105                 110         


Val Asp Leu Ser Ser Leu Glu Glu Thr Val Arg Lys Val Ala Glu Phe 
        115                 120                 125             


Glu Asp Glu Val Ile Phe Arg Gly Cys Glu Lys Ser Gly Val Lys Gly 
    130                 135                 140                 


Leu Leu Ser Phe Glu Glu Arg Lys Ile Glu Cys Gly Ser Thr Pro Lys 
145                 150                 155                 160 


Asp Leu Leu Glu Ala Ile Val Arg Ala Leu Ser Ile Phe Ser Lys Asp 
                165                 170                 175     


Gly Ile Glu Gly Pro Tyr Thr Leu Val Ile Asn Thr Asp Arg Trp Ile 
            180                 185                 190         


Asn Phe Leu Lys Glu Glu Ala Gly His Tyr Pro Leu Glu Lys Arg Val 
        195                 200                 205             


Glu Glu Cys Leu Arg Gly Gly Lys Ile Ile Thr Thr Pro Arg Ile Glu 
    210                 215                 220                 


Asp Ala Leu Val Val Ser Glu Arg Gly Gly Asp Phe Lys Leu Ile Leu 
225                 230                 235                 240 


Gly Gln Asp Leu Ser Ile Gly Tyr Glu Asp Arg Glu Lys Asp Ala Val 
                245                 250                 255     


Arg Leu Phe Ile Thr Glu Thr Phe Thr Met Leu Leu Lys Phe Gly Ser 
            260                 265                 270         


Gly Ser Gly Ser Val Thr Thr Leu Ser Gly Leu Ser Gly Glu Gln Gly 
        275                 280                 285             


Pro Ser Gly Asp Met Thr Thr Glu Glu Asp Ser Ala Thr His Ile Lys 
    290                 295                 300                 


Phe Ser Lys Arg Asp Glu Asp Gly Arg Glu Leu Ala Gly Ala Thr Met 
305                 310                 315                 320 


Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser Thr Trp Ile Ser Asp 
                325                 330                 335     


Gly His Val Lys Asp Phe Tyr Leu Tyr Pro Gly Lys Tyr Thr Phe Val 
            340                 345                 350         


Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala Thr Ala Ile Thr Phe 
        355                 360                 365             


Thr Val Asn Glu Gln Gly Gln Val Thr Val Asn Gly Glu Ala Thr Lys 
    370                 375                 380                 


Gly Asp Ala His Thr Gly Ser Ser Gly Ser 
385                 390                 


