                         SEQUENCE LISTING

<110>  BAYLOR COLLEGE OF MEDICINE
       QUACH, David Hon
       ROONEY, Cliona M.
       RAMOS, Carlos Almeida
 
<120>  Virus-Specific Immune Cells Expressing Chimeric Antigen Receptors

<130>  007921596

<160>  56    

<170>  PatentIn version 3.5

<210>  1
<211>  595
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD30 isoform 1 (UniProt: P28908-1, v1)

<400>  1

Met Arg Val Leu Leu Ala Ala Leu Gly Leu Leu Phe Leu Gly Ala Leu 
1               5                   10                  15      


Arg Ala Phe Pro Gln Asp Arg Pro Phe Glu Asp Thr Cys His Gly Asn 
            20                  25                  30          


Pro Ser His Tyr Tyr Asp Lys Ala Val Arg Arg Cys Cys Tyr Arg Cys 
        35                  40                  45              


Pro Met Gly Leu Phe Pro Thr Gln Gln Cys Pro Gln Arg Pro Thr Asp 
    50                  55                  60                  


Cys Arg Lys Gln Cys Glu Pro Asp Tyr Tyr Leu Asp Glu Ala Asp Arg 
65                  70                  75                  80  


Cys Thr Ala Cys Val Thr Cys Ser Arg Asp Asp Leu Val Glu Lys Thr 
                85                  90                  95      


Pro Cys Ala Trp Asn Ser Ser Arg Val Cys Glu Cys Arg Pro Gly Met 
            100                 105                 110         


Phe Cys Ser Thr Ser Ala Val Asn Ser Cys Ala Arg Cys Phe Phe His 
        115                 120                 125             


Ser Val Cys Pro Ala Gly Met Ile Val Lys Phe Pro Gly Thr Ala Gln 
    130                 135                 140                 


Lys Asn Thr Val Cys Glu Pro Ala Ser Pro Gly Val Ser Pro Ala Cys 
145                 150                 155                 160 


Ala Ser Pro Glu Asn Cys Lys Glu Pro Ser Ser Gly Thr Ile Pro Gln 
                165                 170                 175     


Ala Lys Pro Thr Pro Val Ser Pro Ala Thr Ser Ser Ala Ser Thr Met 
            180                 185                 190         


Pro Val Arg Gly Gly Thr Arg Leu Ala Gln Glu Ala Ala Ser Lys Leu 
        195                 200                 205             


Thr Arg Ala Pro Asp Ser Pro Ser Ser Val Gly Arg Pro Ser Ser Asp 
    210                 215                 220                 


Pro Gly Leu Ser Pro Thr Gln Pro Cys Pro Glu Gly Ser Gly Asp Cys 
225                 230                 235                 240 


Arg Lys Gln Cys Glu Pro Asp Tyr Tyr Leu Asp Glu Ala Gly Arg Cys 
                245                 250                 255     


Thr Ala Cys Val Ser Cys Ser Arg Asp Asp Leu Val Glu Lys Thr Pro 
            260                 265                 270         


Cys Ala Trp Asn Ser Ser Arg Thr Cys Glu Cys Arg Pro Gly Met Ile 
        275                 280                 285             


Cys Ala Thr Ser Ala Thr Asn Ser Cys Ala Arg Cys Val Pro Tyr Pro 
    290                 295                 300                 


Ile Cys Ala Ala Glu Thr Val Thr Lys Pro Gln Asp Met Ala Glu Lys 
305                 310                 315                 320 


Asp Thr Thr Phe Glu Ala Pro Pro Leu Gly Thr Gln Pro Asp Cys Asn 
                325                 330                 335     


Pro Thr Pro Glu Asn Gly Glu Ala Pro Ala Ser Thr Ser Pro Thr Gln 
            340                 345                 350         


Ser Leu Leu Val Asp Ser Gln Ala Ser Lys Thr Leu Pro Ile Pro Thr 
        355                 360                 365             


Ser Ala Pro Val Ala Leu Ser Ser Thr Gly Lys Pro Val Leu Asp Ala 
    370                 375                 380                 


Gly Pro Val Leu Phe Trp Val Ile Leu Val Leu Val Val Val Val Gly 
385                 390                 395                 400 


Ser Ser Ala Phe Leu Leu Cys His Arg Arg Ala Cys Arg Lys Arg Ile 
                405                 410                 415     


Arg Gln Lys Leu His Leu Cys Tyr Pro Val Gln Thr Ser Gln Pro Lys 
            420                 425                 430         


Leu Glu Leu Val Asp Ser Arg Pro Arg Arg Ser Ser Thr Gln Leu Arg 
        435                 440                 445             


Ser Gly Ala Ser Val Thr Glu Pro Val Ala Glu Glu Arg Gly Leu Met 
    450                 455                 460                 


Ser Gln Pro Leu Met Glu Thr Cys His Ser Val Gly Ala Ala Tyr Leu 
465                 470                 475                 480 


Glu Ser Leu Pro Leu Gln Asp Ala Ser Pro Ala Gly Gly Pro Ser Ser 
                485                 490                 495     


Pro Arg Asp Leu Pro Glu Pro Arg Val Ser Thr Glu His Thr Asn Asn 
            500                 505                 510         


Lys Ile Glu Lys Ile Tyr Ile Met Lys Ala Asp Thr Val Ile Val Gly 
        515                 520                 525             


Thr Val Lys Ala Glu Leu Pro Glu Gly Arg Gly Leu Ala Gly Pro Ala 
    530                 535                 540                 


Glu Pro Glu Leu Glu Glu Glu Leu Glu Ala Asp His Thr Pro His Tyr 
545                 550                 555                 560 


Pro Glu Gln Glu Thr Glu Pro Pro Leu Gly Ser Cys Ser Asp Val Met 
                565                 570                 575     


Leu Ser Val Glu Glu Glu Gly Lys Glu Asp Pro Leu Pro Thr Ala Ala 
            580                 585                 590         


Ser Gly Lys 
        595 


<210>  2
<211>  132
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD30 isoform 2 (UniProt: P28908-2)

<400>  2

Met Ser Gln Pro Leu Met Glu Thr Cys His Ser Val Gly Ala Ala Tyr 
1               5                   10                  15      


Leu Glu Ser Leu Pro Leu Gln Asp Ala Ser Pro Ala Gly Gly Pro Ser 
            20                  25                  30          


Ser Pro Arg Asp Leu Pro Glu Pro Arg Val Ser Thr Glu His Thr Asn 
        35                  40                  45              


Asn Lys Ile Glu Lys Ile Tyr Ile Met Lys Ala Asp Thr Val Ile Val 
    50                  55                  60                  


Gly Thr Val Lys Ala Glu Leu Pro Glu Gly Arg Gly Leu Ala Gly Pro 
65                  70                  75                  80  


Ala Glu Pro Glu Leu Glu Glu Glu Leu Glu Ala Asp His Thr Pro His 
                85                  90                  95      


Tyr Pro Glu Gln Glu Thr Glu Pro Pro Leu Gly Ser Cys Ser Asp Val 
            100                 105                 110         


Met Leu Ser Val Glu Glu Glu Gly Lys Glu Asp Pro Leu Pro Thr Ala 
        115                 120                 125             


Ala Ser Gly Lys 
    130         


<210>  3
<211>  483
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD30 isoform 3 (UniProt: P28908-3)

<400>  3

Met Phe Cys Ser Thr Ser Ala Val Asn Ser Cys Ala Arg Cys Phe Phe 
1               5                   10                  15      


His Ser Val Cys Pro Ala Gly Met Ile Val Lys Phe Pro Gly Thr Ala 
            20                  25                  30          


Gln Lys Asn Thr Val Cys Glu Pro Ala Ser Pro Gly Val Ser Pro Ala 
        35                  40                  45              


Cys Ala Ser Pro Glu Asn Cys Lys Glu Pro Ser Ser Gly Thr Ile Pro 
    50                  55                  60                  


Gln Ala Lys Pro Thr Pro Val Ser Pro Ala Thr Ser Ser Ala Ser Thr 
65                  70                  75                  80  


Met Pro Val Arg Gly Gly Thr Arg Leu Ala Gln Glu Ala Ala Ser Lys 
                85                  90                  95      


Leu Thr Arg Ala Pro Asp Ser Pro Ser Ser Val Gly Arg Pro Ser Ser 
            100                 105                 110         


Asp Pro Gly Leu Ser Pro Thr Gln Pro Cys Pro Glu Gly Ser Gly Asp 
        115                 120                 125             


Cys Arg Lys Gln Cys Glu Pro Asp Tyr Tyr Leu Asp Glu Ala Gly Arg 
    130                 135                 140                 


Cys Thr Ala Cys Val Ser Cys Ser Arg Asp Asp Leu Val Glu Lys Thr 
145                 150                 155                 160 


Pro Cys Ala Trp Asn Ser Ser Arg Thr Cys Glu Cys Arg Pro Gly Met 
                165                 170                 175     


Ile Cys Ala Thr Ser Ala Thr Asn Ser Cys Ala Arg Cys Val Pro Tyr 
            180                 185                 190         


Pro Ile Cys Ala Ala Glu Thr Val Thr Lys Pro Gln Asp Met Ala Glu 
        195                 200                 205             


Lys Asp Thr Thr Phe Glu Ala Pro Pro Leu Gly Thr Gln Pro Asp Cys 
    210                 215                 220                 


Asn Pro Thr Pro Glu Asn Gly Glu Ala Pro Ala Ser Thr Ser Pro Thr 
225                 230                 235                 240 


Gln Ser Leu Leu Val Asp Ser Gln Ala Ser Lys Thr Leu Pro Ile Pro 
                245                 250                 255     


Thr Ser Ala Pro Val Ala Leu Ser Ser Thr Gly Lys Pro Val Leu Asp 
            260                 265                 270         


Ala Gly Pro Val Leu Phe Trp Val Ile Leu Val Leu Val Val Val Val 
        275                 280                 285             


Gly Ser Ser Ala Phe Leu Leu Cys His Arg Arg Ala Cys Arg Lys Arg 
    290                 295                 300                 


Ile Arg Gln Lys Leu His Leu Cys Tyr Pro Val Gln Thr Ser Gln Pro 
305                 310                 315                 320 


Lys Leu Glu Leu Val Asp Ser Arg Pro Arg Arg Ser Ser Thr Leu Arg 
                325                 330                 335     


Ser Gly Ala Ser Val Thr Glu Pro Val Ala Glu Glu Arg Gly Leu Met 
            340                 345                 350         


Ser Gln Pro Leu Met Glu Thr Cys His Ser Val Gly Ala Ala Tyr Leu 
        355                 360                 365             


Glu Ser Leu Pro Leu Gln Asp Ala Ser Pro Ala Gly Gly Pro Ser Ser 
    370                 375                 380                 


Pro Arg Asp Leu Pro Glu Pro Arg Val Ser Thr Glu His Thr Asn Asn 
385                 390                 395                 400 


Lys Ile Glu Lys Ile Tyr Ile Met Lys Ala Asp Thr Val Ile Val Gly 
                405                 410                 415     


Thr Val Lys Ala Glu Leu Pro Glu Gly Arg Gly Leu Ala Gly Pro Ala 
            420                 425                 430         


Glu Pro Glu Leu Glu Glu Glu Leu Glu Ala Asp His Thr Pro His Tyr 
        435                 440                 445             


Pro Glu Gln Glu Thr Glu Pro Pro Leu Gly Ser Cys Ser Asp Val Met 
    450                 455                 460                 


Leu Ser Val Glu Glu Glu Gly Lys Glu Asp Pro Leu Pro Thr Ala Ala 
465                 470                 475                 480 


Ser Gly Lys 
            


<210>  4
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD30 signal peptide

<400>  4

Met Arg Val Leu Leu Ala Ala Leu Gly Leu Leu Phe Leu Gly Ala Leu 
1               5                   10                  15      


Arg Ala 
        


<210>  5
<211>  367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD30 extracellular domain

<400>  5

Phe Pro Gln Asp Arg Pro Phe Glu Asp Thr Cys His Gly Asn Pro Ser 
1               5                   10                  15      


His Tyr Tyr Asp Lys Ala Val Arg Arg Cys Cys Tyr Arg Cys Pro Met 
            20                  25                  30          


Gly Leu Phe Pro Thr Gln Gln Cys Pro Gln Arg Pro Thr Asp Cys Arg 
        35                  40                  45              


Lys Gln Cys Glu Pro Asp Tyr Tyr Leu Asp Glu Ala Asp Arg Cys Thr 
    50                  55                  60                  


Ala Cys Val Thr Cys Ser Arg Asp Asp Leu Val Glu Lys Thr Pro Cys 
65                  70                  75                  80  


Ala Trp Asn Ser Ser Arg Val Cys Glu Cys Arg Pro Gly Met Phe Cys 
                85                  90                  95      


Ser Thr Ser Ala Val Asn Ser Cys Ala Arg Cys Phe Phe His Ser Val 
            100                 105                 110         


Cys Pro Ala Gly Met Ile Val Lys Phe Pro Gly Thr Ala Gln Lys Asn 
        115                 120                 125             


Thr Val Cys Glu Pro Ala Ser Pro Gly Val Ser Pro Ala Cys Ala Ser 
    130                 135                 140                 


Pro Glu Asn Cys Lys Glu Pro Ser Ser Gly Thr Ile Pro Gln Ala Lys 
145                 150                 155                 160 


Pro Thr Pro Val Ser Pro Ala Thr Ser Ser Ala Ser Thr Met Pro Val 
                165                 170                 175     


Arg Gly Gly Thr Arg Leu Ala Gln Glu Ala Ala Ser Lys Leu Thr Arg 
            180                 185                 190         


Ala Pro Asp Ser Pro Ser Ser Val Gly Arg Pro Ser Ser Asp Pro Gly 
        195                 200                 205             


Leu Ser Pro Thr Gln Pro Cys Pro Glu Gly Ser Gly Asp Cys Arg Lys 
    210                 215                 220                 


Gln Cys Glu Pro Asp Tyr Tyr Leu Asp Glu Ala Gly Arg Cys Thr Ala 
225                 230                 235                 240 


Cys Val Ser Cys Ser Arg Asp Asp Leu Val Glu Lys Thr Pro Cys Ala 
                245                 250                 255     


Trp Asn Ser Ser Arg Thr Cys Glu Cys Arg Pro Gly Met Ile Cys Ala 
            260                 265                 270         


Thr Ser Ala Thr Asn Ser Cys Ala Arg Cys Val Pro Tyr Pro Ile Cys 
        275                 280                 285             


Ala Ala Glu Thr Val Thr Lys Pro Gln Asp Met Ala Glu Lys Asp Thr 
    290                 295                 300                 


Thr Phe Glu Ala Pro Pro Leu Gly Thr Gln Pro Asp Cys Asn Pro Thr 
305                 310                 315                 320 


Pro Glu Asn Gly Glu Ala Pro Ala Ser Thr Ser Pro Thr Gln Ser Leu 
                325                 330                 335     


Leu Val Asp Ser Gln Ala Ser Lys Thr Leu Pro Ile Pro Thr Ser Ala 
            340                 345                 350         


Pro Val Ala Leu Ser Ser Thr Gly Lys Pro Val Leu Asp Ala Gly 
        355                 360                 365         


<210>  6
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD30 transmembrane domain

<400>  6

Pro Val Leu Phe Trp Val Ile Leu Val Leu Val Val Val Val Gly Ser 
1               5                   10                  15      


Ser Ala Phe Leu Leu 
            20      


<210>  7
<211>  189
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD30 cytoplasmic domain

<400>  7

Cys His Arg Arg Ala Cys Arg Lys Arg Ile Arg Gln Lys Leu His Leu 
1               5                   10                  15      


Cys Tyr Pro Val Gln Thr Ser Gln Pro Lys Leu Glu Leu Val Asp Ser 
            20                  25                  30          


Arg Pro Arg Arg Ser Ser Thr Gln Leu Arg Ser Gly Ala Ser Val Thr 
        35                  40                  45              


Glu Pro Val Ala Glu Glu Arg Gly Leu Met Ser Gln Pro Leu Met Glu 
    50                  55                  60                  


Thr Cys His Ser Val Gly Ala Ala Tyr Leu Glu Ser Leu Pro Leu Gln 
65                  70                  75                  80  


Asp Ala Ser Pro Ala Gly Gly Pro Ser Ser Pro Arg Asp Leu Pro Glu 
                85                  90                  95      


Pro Arg Val Ser Thr Glu His Thr Asn Asn Lys Ile Glu Lys Ile Tyr 
            100                 105                 110         


Ile Met Lys Ala Asp Thr Val Ile Val Gly Thr Val Lys Ala Glu Leu 
        115                 120                 125             


Pro Glu Gly Arg Gly Leu Ala Gly Pro Ala Glu Pro Glu Leu Glu Glu 
    130                 135                 140                 


Glu Leu Glu Ala Asp His Thr Pro His Tyr Pro Glu Gln Glu Thr Glu 
145                 150                 155                 160 


Pro Pro Leu Gly Ser Cys Ser Asp Val Met Leu Ser Val Glu Glu Glu 
                165                 170                 175     


Gly Lys Glu Asp Pro Leu Pro Thr Ala Ala Ser Gly Lys 
            180                 185                 


<210>  8
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HRS3 HC-CDR1

<400>  8

Gly Tyr Thr Phe Thr Thr Tyr Thr 
1               5               


<210>  9
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HRS3 HC-CDR2

<400>  9

Ile Asn Pro Ser Ser Gly Cys Ser 
1               5               


<210>  10
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HRS3 HC-CDR3

<400>  10

Ala Arg Arg Ala Asp Tyr Gly Asn Tyr Glu Tyr Thr Trp Phe Ala Tyr 
1               5                   10                  15      


<210>  11
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HRS3 LC-CDR1

<400>  11

Gln Asn Val Gly Thr Asn 
1               5       


<210>  12

<400>  12
000

<210>  13
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HRS3 LC-CDR3

<400>  13

Gln Gln Tyr His Thr Tyr Pro Leu Thr 
1               5                   


<210>  14
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HRS3 VH

<400>  14

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Thr Tyr 
            20                  25                  30          


Thr Ile His Trp Val Arg Arg Arg Pro Gly His Asp Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Ser Gly Cys Ser Asp Tyr Asn Gln Asn Phe 
    50                  55                  60                  


Lys Gly Lys Thr Thr Leu Thr Ala Asp Lys Ser Ser Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Ala Asp Tyr Gly Asn Tyr Glu Tyr Thr Trp Phe Ala Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120             


<210>  15
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HRS3 VL

<400>  15

Val Ile Glu Leu Thr Gln Ser Pro Lys Phe Met Ser Thr Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Asn Val Thr Tyr Lys Ala Ser Gln Asn Val Gly Thr Asn 
            20                  25                  30          


Val Ala Trp Phe Gln Gln Lys Pro Gly Gln Ser Pro Lys Val Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Asn Val Gln Ser 
65                  70                  75                  80  


Glu Asp Leu Ala Glu Tyr Phe Cys Gln Gln Tyr His Thr Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  16
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G4S

<400>  16

Gly Gly Gly Gly Ser 
1               5   


<210>  17
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HRS3 scFv linker

<400>  17

Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  18
<211>  245
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HRS3 scFv

<400>  18

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Thr Tyr 
            20                  25                  30          


Thr Ile His Trp Val Arg Arg Arg Pro Gly His Asp Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Ser Gly Cys Ser Asp Tyr Asn Gln Asn Phe 
    50                  55                  60                  


Lys Gly Lys Thr Thr Leu Thr Ala Asp Lys Ser Ser Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Ala Asp Tyr Gly Asn Tyr Glu Tyr Thr Trp Phe Ala Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ser Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Val Ile Glu Leu Thr Gln 
    130                 135                 140                 


Ser Pro Lys Phe Met Ser Thr Ser Val Gly Asp Arg Val Asn Val Thr 
145                 150                 155                 160 


Tyr Lys Ala Ser Gln Asn Val Gly Thr Asn Val Ala Trp Phe Gln Gln 
                165                 170                 175     


Lys Pro Gly Gln Ser Pro Lys Val Leu Ile Tyr Ser Ala Ser Tyr Arg 
            180                 185                 190         


Tyr Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp 
        195                 200                 205             


Phe Thr Leu Thr Ile Ser Asn Val Gln Ser Glu Asp Leu Ala Glu Tyr 
    210                 215                 220                 


Phe Cys Gln Gln Tyr His Thr Tyr Pro Leu Thr Phe Gly Gly Gly Thr 
225                 230                 235                 240 


Lys Leu Glu Ile Lys 
                245 


<210>  19
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HRS3 epitope

<400>  19

Ala Thr Ser Ser Ala Ser Thr Met Pro Val Arg Gly Gly Thr Arg Leu 
1               5                   10                  15      


Ala Gln Glu Ala Ala Ser Lys Leu Thr Arg Ala Pro Asp Ser Pro Ser 
            20                  25                  30          


Ser Val Gly Arg Pro Ser Ser Asp Pro Gly Leu Ser Pro Thr Gln Pro 
        35                  40                  45              


Cys Pro Glu Gly Ser Gly Asp Cys Arg Lys Gln Cys Glu Pro Asp Tyr 
    50                  55                  60                  


Tyr Leu Asp Glu Ala Gly Arg Cys Thr Ala Cys Val Ser Cys Ser Arg 
65                  70                  75                  80  


Asp Asp Leu Val Glu Lys Thr Pro Cys Ala Trp Asn Ser Ser Arg Thr 
                85                  90                  95      


Cys Glu Cys Arg Pro Gly Met Ile Cys Ala Thr Ser Ala Thr Asn Ser 
            100                 105                 110         


Cys Ala Arg Cys Val Pro Tyr Pro Ile Cys Ala Ala Glu Thr Val Thr 
        115                 120                 125             


Lys Pro Gln Asp Met Ala Glu Lys Asp Thr Thr Phe Glu Ala Pro Pro 
    130                 135                 140                 


Leu Gly Thr Gln Pro Asp Cys 
145                 150     


<210>  20
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD28 transmembrane domain

<400>  20

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile 
            20                  


<210>  21
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD3Zeta transmembrane domain

<400>  21

Leu Cys Tyr Leu Leu Asp Gly Ile Leu Phe Ile Tyr Gly Val Ile Leu 
1               5                   10                  15      


Thr Ala Leu Phe Leu 
            20      


<210>  22
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD8Alpha transmembrane domain

<400>  22

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
            20                  25              


<210>  23
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITAM consensus


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa = Leucine or Isoleucine

<400>  23

Tyr Xaa Xaa Xaa 
1               


<210>  24
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Larger ITAM consensus


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa = Leucine or Isoleucine

<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (12)..(13)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa = Leucine or Isoleucine

<400>  24

Tyr Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Tyr Xaa Xaa Xaa 
1               5                   10                  


<210>  25
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD3Zeta intracellular domain

<400>  25

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  26
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD28 intracellular domain

<400>  26

Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
1               5                   10                  15      


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
            20                  25                  30          


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40                  


<210>  27
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD28 intracellular domain with mutated lck binding site

<400>  27

Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
1               5                   10                  15      


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Ala 
            20                  25                  30          


Tyr Ala Ala Ala Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40                  


<210>  28
<211>  156
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR signalling domain 1

<400>  28

Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
1               5                   10                  15      


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
            20                  25                  30          


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe 
        35                  40                  45              


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
    50                  55                  60                  


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
65                  70                  75                  80  


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
                85                  90                  95      


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
            100                 105                 110         


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
        115                 120                 125             


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
    130                 135                 140                 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150                 155     


<210>  29
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 CH1-CH2 hinge region

<400>  29

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro 
1               5                   10          


<210>  30
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 CH1-CH2 hinge region C103P variant

<400>  30

Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro 
1               5                   10          


<210>  31
<211>  220
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 CH2-CH3 hinge region

<400>  31

Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
1               5                   10                  15      


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
            20                  25                  30          


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
        35                  40                  45              


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
    50                  55                  60                  


Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
65                  70                  75                  80  


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
                85                  90                  95      


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
            100                 105                 110         


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
        115                 120                 125             


Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
    130                 135                 140                 


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
145                 150                 155                 160 


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
                165                 170                 175     


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
            180                 185                 190         


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
        195                 200                 205             


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215                 220 


<210>  32
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 CH2-CH3 hinge region variant

<400>  32

Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro 
1               5                   10                  15      


Pro Lys Pro Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr 
            20                  25                  30          


Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 
        35                  40                  45              


Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
    50                  55                  60                  


Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 
65                  70                  75                  80  


Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 
                85                  90                  95      


Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
            100                 105                 110         


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp 
        115                 120                 125             


Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
    130                 135                 140                 


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
145                 150                 155                 160 


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
                165                 170                 175     


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
            180                 185                 190         


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
        195                 200                 205             


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys 
    210                 215                 220             


<210>  33
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR hinge region

<400>  33

Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  34
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal peptide 1

<400>  34

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 
1               5                   10                  15      


Val Ile Met Ser 
            20  


<210>  35
<211>  666
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  http://CD30.CAR (lacking signal peptide)

<400>  35

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Thr Tyr 
            20                  25                  30          


Thr Ile His Trp Val Arg Arg Arg Pro Gly His Asp Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Ser Gly Cys Ser Asp Tyr Asn Gln Asn Phe 
    50                  55                  60                  


Lys Gly Lys Thr Thr Leu Thr Ala Asp Lys Ser Ser Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Ala Asp Tyr Gly Asn Tyr Glu Tyr Thr Trp Phe Ala Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ser Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Val Ile Glu Leu Thr Gln 
    130                 135                 140                 


Ser Pro Lys Phe Met Ser Thr Ser Val Gly Asp Arg Val Asn Val Thr 
145                 150                 155                 160 


Tyr Lys Ala Ser Gln Asn Val Gly Thr Asn Val Ala Trp Phe Gln Gln 
                165                 170                 175     


Lys Pro Gly Gln Ser Pro Lys Val Leu Ile Tyr Ser Ala Ser Tyr Arg 
            180                 185                 190         


Tyr Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp 
        195                 200                 205             


Phe Thr Leu Thr Ile Ser Asn Val Gln Ser Glu Asp Leu Ala Glu Tyr 
    210                 215                 220                 


Phe Cys Gln Gln Tyr His Thr Tyr Pro Leu Thr Phe Gly Gly Gly Thr 
225                 230                 235                 240 


Lys Leu Glu Ile Lys Arg Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp 
                245                 250                 255     


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
            260                 265                 270         


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
        275                 280                 285             


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
    290                 295                 300                 


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
305                 310                 315                 320 


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
                325                 330                 335     


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
            340                 345                 350         


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
        355                 360                 365             


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
    370                 375                 380                 


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
385                 390                 395                 400 


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
                405                 410                 415     


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
            420                 425                 430         


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
        435                 440                 445             


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
    450                 455                 460                 


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
465                 470                 475                 480 


Gly Lys Lys Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val 
                485                 490                 495     


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
            500                 505                 510         


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
        515                 520                 525             


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
    530                 535                 540                 


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg 
545                 550                 555                 560 


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
                565                 570                 575     


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
            580                 585                 590         


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
        595                 600                 605             


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
    610                 615                 620                 


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
625                 630                 635                 640 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
                645                 650                 655     


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            660                 665     


<210>  36
<211>  689
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  http://CD30.CAR (with signal peptide)

<400>  36

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 
1               5                   10                  15      


Val Ile Met Ser Arg Met Ala Gln Val Gln Leu Gln Gln Ser Gly Ala 
            20                  25                  30          


Glu Leu Ala Arg Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser 
        35                  40                  45              


Gly Tyr Thr Phe Thr Thr Tyr Thr Ile His Trp Val Arg Arg Arg Pro 
    50                  55                  60                  


Gly His Asp Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Ser Gly Cys 
65                  70                  75                  80  


Ser Asp Tyr Asn Gln Asn Phe Lys Gly Lys Thr Thr Leu Thr Ala Asp 
                85                  90                  95      


Lys Ser Ser Asn Thr Ala Tyr Met Gln Leu Asn Ser Leu Thr Ser Glu 
            100                 105                 110         


Asp Ser Ala Val Tyr Tyr Cys Ala Arg Arg Ala Asp Tyr Gly Asn Tyr 
        115                 120                 125             


Glu Tyr Thr Trp Phe Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val 
    130                 135                 140                 


Ser Ser Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Val Ile Glu Leu Thr Gln Ser Pro Lys Phe Met Ser Thr Ser Val 
                165                 170                 175     


Gly Asp Arg Val Asn Val Thr Tyr Lys Ala Ser Gln Asn Val Gly Thr 
            180                 185                 190         


Asn Val Ala Trp Phe Gln Gln Lys Pro Gly Gln Ser Pro Lys Val Leu 
        195                 200                 205             


Ile Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Thr 
    210                 215                 220                 


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Asn Val Gln 
225                 230                 235                 240 


Ser Glu Asp Leu Ala Glu Tyr Phe Cys Gln Gln Tyr His Thr Tyr Pro 
                245                 250                 255     


Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ser Asp Pro 
            260                 265                 270         


Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        275                 280                 285             


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
    290                 295                 300                 


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
305                 310                 315                 320 


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
                325                 330                 335     


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
            340                 345                 350         


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
        355                 360                 365             


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
    370                 375                 380                 


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
385                 390                 395                 400 


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
                405                 410                 415     


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
            420                 425                 430         


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
        435                 440                 445             


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
    450                 455                 460                 


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
465                 470                 475                 480 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
                485                 490                 495     


Lys Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys Phe Trp Val 
            500                 505                 510         


Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr 
        515                 520                 525             


Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu 
    530                 535                 540                 


His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg 
545                 550                 555                 560 


Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg 
                565                 570                 575     


Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
            580                 585                 590         


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
        595                 600                 605             


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
    610                 615                 620                 


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
625                 630                 635                 640 


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
                645                 650                 655     


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
            660                 665                 670         


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
        675                 680                 685             


Arg 
    


<210>  37
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FMC63 HC-CDR1

<400>  37

Gly Val Ser Leu Pro Asp Tyr Gly 
1               5               


<210>  38
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FMC63 HC-CDR2

<400>  38

Ile Trp Gly Ser Glu Thr Thr 
1               5           


<210>  39
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FMC63 HC-CDR3

<400>  39

Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
1               5                   10                  


<210>  40
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FMC63 LC-CDR1

<400>  40

Gln Asp Ile Ser Lys Tyr 
1               5       


<210>  41

<400>  41
000

<210>  42
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FMC63 LC-CDR3

<400>  42

Gln Gln Gly Asn Thr Leu Pro Tyr Thr 
1               5                   


<210>  43
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FMC63 VH

<400>  43

Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
            20                  25                  30          


Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  


Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  


Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala 
                85                  90                  95      


Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser 
        115                 120 


<210>  44
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FMC63 VL

<400>  44

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
            100                 105         


<210>  45
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (G4S)3 linker

<400>  45

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  46
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FMC63 scFv

<400>  46

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser 
            260             


<210>  47
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG Hinge 3

<400>  47

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Asp Pro Lys 
1               5                   10                  15  


<210>  48
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD28 transmembrane domain v2

<400>  48

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser 
            20                  25                  


<210>  49
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human 4-1BB intracellular domain v2

<400>  49

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  50
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR signalling domain 2

<400>  50

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
        35                  40                  45              


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
    50                  55                  60                  


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
65                  70                  75                  80  


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
                85                  90                  95      


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
            100                 105                 110         


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
        115                 120                 125             


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
    130                 135                 140                 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150                 


<210>  51
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal peptide 2

<400>  51

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  52
<211>  440
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  http://CD19.CAR (lacking signal peptide)

<400>  52

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu 
        115                 120                 125             


Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys 
    130                 135                 140                 


Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg 
145                 150                 155                 160 


Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser 
                165                 170                 175     


Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile 
            180                 185                 190         


Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 
        195                 200                 205             


Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly 
    210                 215                 220                 


Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 
225                 230                 235                 240 


Ser Ser Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Asp Pro 
                245                 250                 255     


Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
            260                 265                 270         


Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg 
        275                 280                 285             


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
    290                 295                 300                 


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
305                 310                 315                 320 


Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
                325                 330                 335     


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
            340                 345                 350         


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
        355                 360                 365             


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
    370                 375                 380                 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
385                 390                 395                 400 


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
                405                 410                 415     


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
            420                 425                 430         


His Met Gln Ala Leu Pro Pro Arg 
        435                 440 


<210>  53
<211>  461
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  http://CD19.CAR (with signal peptide)

<400>  53

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Glu Ser Lys Tyr Gly Pro Pro Cys Pro 
            260                 265                 270         


Pro Cys Pro Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val 
        275                 280                 285             


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
    290                 295                 300                 


Val Arg Ser Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
305                 310                 315                 320 


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
                325                 330                 335     


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
            340                 345                 350         


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
        355                 360                 365             


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
    370                 375                 380                 


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
385                 390                 395                 400 


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
                405                 410                 415     


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
            420                 425                 430         


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
        435                 440                 445             


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460     


<210>  54
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Vectofusin-1

<400>  54

Lys Lys Ala Leu Leu His Ala Ala Leu Ala His Leu Leu Ala Leu Ala 
1               5                   10                  15      


His His Leu Leu Ala Leu Leu Lys Lys Ala 
            20                  25      


<210>  55
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Larger ITAM consensus


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa = Leucine or Isoleucine

<220>
<221>  misc_feature
<222>  (5)..(11)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (13)..(14)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa = Leucine or Isoleucine

<400>  55

Tyr Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Tyr Xaa Xaa Xaa 
1               5                   10                  15  


<210>  56
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Larger ITAM consensus


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa = Leucine or Isoleucine

<220>
<221>  misc_feature
<222>  (5)..(12)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (16)..(16)
<223>  Xaa = Leucine or Isoleucine

<400>  56

Tyr Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Tyr Xaa Xaa Xaa 
1               5                   10                  15      


