                         SEQUENCE LISTING

<110>  Ustav organicke chemie a biochemie AV CR, et al.
 
<120>  Antibody binding to RBD of Spike protein of SARS-CoV-2 and a 
       method for quantifying protective antibodies against SARS-CoV-2

<130>  P

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  122
<212>  PRT
<213>  artificial

<220>
<223>  VHC region

<400>  1

Glu Val Gln Leu Arg Glu Ser Gly Pro Gly Ile Leu Gln Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ser Phe Ser Gly Phe Ser Leu Ser Thr Ser 
            20                  25                  30          


Gly Met Gly Val Ser Trp Ile Arg Gln Pro Ser Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Leu Ala His Ile Tyr Trp Asp Asp Asp Glu Arg Tyr Asn Pro Ser 
    50                  55                  60                  


Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Val Ser Ser Asn Gln Val 
65                  70                  75                  80  


Phe Leu Lys Ile Ile Ser Val Asp Thr Ala Asp Thr Ala Thr Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Arg Ser Leu Tyr Asp Tyr Asp Gly Trp Phe Ala Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Leu Val Thr Val Ser Ala 
        115                 120         


<210>  2
<211>  109
<212>  PRT
<213>  artificial

<220>
<223>  VLC region

<400>  2

Asp Ile Val Met Thr Gln Ser Gln Lys Phe Met Ser Thr Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Ser Val Thr Cys Lys Ala Ser Gln Asn Val Asp Thr Thr 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Ala Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Ser Arg Tyr Ser Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Asn Val Gln Ser 
65                  70                  75                  80  


Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Asn Asn Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Ile Lys Arg Ala 
            100                 105                 


<210>  3
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  CDR

<400>  3

Thr Ser Gly Met Gly Val Ser 
1               5           


<210>  4
<211>  16
<212>  PRT
<213>  artificial

<220>
<223>  CDR

<400>  4

His Ile Tyr Trp Asp Asp Asp Glu Arg Tyr Asn Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  5
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  CDR

<400>  5

Arg Ser Leu Tyr Asp Tyr Asp Gly Trp Phe Ala Tyr 
1               5                   10          


<210>  6
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  CDR

<400>  6

Lys Ala Ser Gln Asn Val Asp Thr Thr Val Ala 
1               5                   10      


<210>  7
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  CDR

<400>  7

Ser Ala Ser Ser Arg Tyr Ser 
1               5           


<210>  8
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  CDR

<400>  8

Gln Gln Tyr Asn Asn Tyr Pro Leu Thr 
1               5                   


<210>  9
<211>  1311
<212>  PRT
<213>  artificial

<220>
<223>  Full-length Spike

<400>  9

Ala Ala Gln Pro Ala Arg Arg Ala Arg Arg Thr Lys Leu Gly Thr Glu 
1               5                   10                  15      


Leu Gly Ser Gly Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            20                  25                  30          


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro 
        35                  40                  45              


Gln Phe Glu Lys Gly Gly Gly Ser Leu Glu Val Leu Phe Gln Gly Pro 
    50                  55                  60                  


Ala Ala Ala Met Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn 
65                  70                  75                  80  


Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser 
                85                  90                  95      


Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val 
            100                 105                 110         


Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg 
        115                 120                 125             


Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser 
    130                 135                 140                 


Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu 
145                 150                 155                 160 


Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val 
                165                 170                 175     


Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly 
            180                 185                 190         


Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg 
        195                 200                 205             


Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro 
    210                 215                 220                 


Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg 
225                 230                 235                 240 


Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys 
                245                 250                 255     


His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala 
            260                 265                 270         


Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe 
        275                 280                 285             


Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser 
    290                 295                 300                 


Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu 
305                 310                 315                 320 


Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr 
                325                 330                 335     


Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr 
            340                 345                 350         


Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe 
        355                 360                 365             


Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
    370                 375                 380                 


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
385                 390                 395                 400 


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
                405                 410                 415     


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
            420                 425                 430         


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
        435                 440                 445             


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
    450                 455                 460                 


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
465                 470                 475                 480 


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
                485                 490                 495     


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
            500                 505                 510         


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
        515                 520                 525             


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
    530                 535                 540                 


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
545                 550                 555                 560 


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
                565                 570                 575     


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn 
            580                 585                 590         


Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys 
        595                 600                 605             


Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp 
    610                 615                 620                 


Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys 
625                 630                 635                 640 


Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn 
                645                 650                 655     


Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val 
            660                 665                 670         


Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr 
        675                 680                 685             


Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu 
    690                 695                 700                 


His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile 
705                 710                 715                 720 


Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Ser Ser 
                725                 730                 735     


Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu 
            740                 745                 750         


Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe 
        755                 760                 765             


Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr 
    770                 775                 780                 


Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser 
785                 790                 795                 800 


Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala 
                805                 810                 815     


Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe 
            820                 825                 830         


Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly 
        835                 840                 845             


Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys 
    850                 855                 860                 


Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp 
865                 870                 875                 880 


Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala 
                885                 890                 895     


Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro 
            900                 905                 910         


Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu 
        915                 920                 925             


Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu 
    930                 935                 940                 


Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly 
945                 950                 955                 960 


Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln 
                965                 970                 975     


Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala 
            980                 985                 990         


Ser Ala Leu Gly Lys Leu Gln Asp  Val Val Asn Gln Asn  Ala Gln Ala 
        995                 1000                 1005             


Leu Asn  Thr Leu Val Lys Gln  Leu Ser Ser Asn Phe  Gly Ala Ile 
    1010                 1015                 1020             


Ser Ser  Val Leu Asn Asp Ile  Leu Ser Arg Leu Asp  Pro Pro Glu 
    1025                 1030                 1035             


Ala Glu  Val Gln Ile Asp Arg  Leu Ile Thr Gly Arg  Leu Gln Ser 
    1040                 1045                 1050             


Leu Gln  Thr Tyr Val Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile 
    1055                 1060                 1065             


Arg Ala  Ser Ala Asn Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val 
    1070                 1075                 1080             


Leu Gly  Gln Ser Lys Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His 
    1085                 1090                 1095             


Leu Met  Ser Phe Pro Gln Ser  Ala Pro His Gly Val  Val Phe Leu 
    1100                 1105                 1110             


His Val  Thr Tyr Val Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala 
    1115                 1120                 1125             


Pro Ala  Ile Cys His Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly 
    1130                 1135                 1140             


Val Phe  Val Ser Asn Gly Thr  His Trp Phe Val Thr  Gln Arg Asn 
    1145                 1150                 1155             


Phe Tyr  Glu Pro Gln Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser 
    1160                 1165                 1170             


Gly Asn  Cys Asp Val Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr 
    1175                 1180                 1185             


Asp Pro  Leu Gln Pro Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp 
    1190                 1195                 1200             


Lys Tyr  Phe Lys Asn His Thr  Ser Pro Asp Val Asp  Leu Gly Asp 
    1205                 1210                 1215             


Ile Ser  Gly Ile Asn Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile 
    1220                 1225                 1230             


Asp Arg  Leu Asn Glu Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile 
    1235                 1240                 1245             


Asp Leu  Gln Glu Leu Gly Lys  Tyr Glu Gln Gly Ser  Gly Tyr Ile 
    1250                 1255                 1260             


Pro Glu  Ala Pro Arg Asp Gly  Gln Ala Tyr Val Arg  Lys Asp Gly 
    1265                 1270                 1275             


Glu Trp  Val Leu Leu Ser Thr  Phe Leu Gly Gly Ala  Pro Leu Glu 
    1280                 1285                 1290             


Gly Gly  Gly Gly Leu Asn Asp  Ile Phe Glu Ala Gln  Lys Ile Glu 
    1295                 1300                 1305             


Trp His  Glu 
    1310     


<210>  10
<211>  289
<212>  PRT
<213>  artificial

<220>
<223>  RBD domain

<400>  10

Ala Ala Gln Pro Ala Arg Arg Ala Arg Arg Thr Lys Leu Gly Thr Glu 
1               5                   10                  15      


Leu Gly Ser Gly Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            20                  25                  30          


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro 
        35                  40                  45              


Gln Phe Glu Lys Gly Gly Gly Ser Leu Glu Val Leu Phe Gln Gly Pro 
    50                  55                  60                  


Ala Ala Ala Met Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn 
65                  70                  75                  80  


Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser 
                85                  90                  95      


Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser 
            100                 105                 110         


Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys 
        115                 120                 125             


Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val 
    130                 135                 140                 


Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr 
145                 150                 155                 160 


Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn 
                165                 170                 175     


Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu 
            180                 185                 190         


Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu 
        195                 200                 205             


Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn 
    210                 215                 220                 


Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val 
225                 230                 235                 240 


Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His 
                245                 250                 255     


Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Gly Ala Pro Leu Glu Gly 
            260                 265                 270         


Gly Gly Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His 
        275                 280                 285             


Glu 
    


