                         SEQUENCE LISTING

<110>  ABL BIO Incorporated
       AHN, Jinhyung et al.
 
<120>  ANTIBODIES FOR TREATING ALPHA-SYNUCLEINOPATHIES

<130>  122548.WO015

<150>  KR 10-2021-0061407
<151>  2021-05-12

<160>  77    

<170>  PatentIn version 3.5

<210>  1
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.1)

<400>  1

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Leu Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Val Ile Asn Pro Gly Ser Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Gly Asn Tyr Asp Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  2
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.2)

<400>  2

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Leu Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Val Ile Asn Pro Gly Ser Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Val Thr Leu Thr Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Gly Asn Tyr Asp Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  3
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.3)

<400>  3

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ala Phe Thr Asn Tyr 
            20                  25                  30          


Leu Ile Glu Trp Val Lys Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Val Ile Asn Pro Gly Ser Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Val Thr Leu Thr Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Gly Asn Tyr Asp Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  4
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.4)

<400>  4

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ala Phe Thr Asn Tyr 
            20                  25                  30          


Leu Ile Glu Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Val Ile Asn Pro Gly Ser Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Gly Asn Tyr Asp Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  5
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.5)

<400>  5

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ala Phe Thr Asn Tyr 
            20                  25                  30          


Leu Ile Glu Trp Val Lys Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Val Ile Asn Pro Gly Ser Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Ser Gly Asn Tyr Asp Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  6
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.6)

<400>  6

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ala Phe Thr Asn Tyr 
            20                  25                  30          


Leu Ile Glu Trp Val Lys Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Val Ile Asn Pro Gly Ser Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Asp Thr Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Ser Gly Asn Tyr Asp Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  7
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.7)

<400>  7

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ala Phe Thr Asn Tyr 
            20                  25                  30          


Leu Ile Glu Trp Val Lys Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Val Ile Asn Pro Gly Ser Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Gly Asn Tyr Asp Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  8
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.8)

<400>  8

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Leu Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Val Ile Asn Pro Gly Ser Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Gly Asn Tyr Asp Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  9
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.9)

<400>  9

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Leu Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Val Ile Asn Pro Gly Ser Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Gly Asn Tyr Asp Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  10
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: ch1E4 (CPF)_VH

<400>  10

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Thr 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ala Phe Thr Asn Tyr 
            20                  25                  30          


Leu Ile Glu Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Val Ile Asn Pro Gly Ser Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Asp Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Ser Gly Asn Tyr Asp Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala 
        115 


<210>  11
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (VL1)

<400>  11

Asp Ile Val Met Thr Gln Thr Pro Leu Ser Leu Ser Val Thr Pro Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Arg Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  12
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (VL2)

<400>  12

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Ser Val Thr Pro Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Arg Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  13
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (VL3)

<400>  13

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Phe Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Arg Arg Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Arg Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


<210>  14
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (VL4)

<400>  14

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Arg Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


<210>  15
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (VL5)

<400>  15

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Arg Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


<210>  16
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: ch1E4 (CPF)_VL

<400>  16

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  17
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.1)

<400>  17
caggtgcaac tggtccaatc tggtgccgaa gtgaaaaagc ccggtgcaag tgttaaagtt       60

tcctgcaaag catctggcta tacttttact aattatctga tagaatgggt acgacaagct      120

ccaggtcaag gtcttgaatg gatgggcgtg attaacccag ggagtggagg aactaattac      180

aatgagaagt tcaaaggacg agtgacaatg acccgtgata ccagtattag caccgcatac      240

atggagttga gcaggcttcg tagcgacgac accgcagtat attattgtgc ttccggcaat      300

tatgatacct attgggggca ggggactctc gtaaccgtct ccagt                      345


<210>  18
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.2)

<400>  18
caagttcagc ttgtgcaaag cggagctgag gtcaaaaaac caggagcatc tgtgaaagtg       60

tcatgcaaag catctggtta cacttttact aactatctca ttgagtgggt cagacaggct      120

ccaggacaag ggttggaatg gattggggtc atcaaccctg ggtcaggggg gacaaattac      180

aatgaaaaat ttaaagggaa ggtgacactc acagcagata agtctatttc aaccgcttat      240

atggaacttt cccgccttag gagcgatgat acagcagtct attattgcgc ttcagggaac      300

tacgacactt actgggggca aggcacactc gttacagtgt cctcc                      345


<210>  19
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.3)

<400>  19
caagtgcagc tggtgcagtc tggcgcagaa gtgaagaaac ctggggcttc tgtgaaggtc       60

agctgtaaag cttctgggta tgcttttaca aactatctca tagaatgggt caaacaagct      120

ccaggtcagg gcctggagtg gatcggtgta atcaaccctg gtagtggcgg aacaaactac      180

aatgagaagt tcaaaggcaa agtgactctt accgccgaca aaagcatttc tacagcttat      240

atggagttgt caaggttgag aagcgacgac acagcagttt actattgcgc ttcaggaaat      300

tatgacacat actggggtca gggtacactg gtgactgtgt cctct                      345


<210>  20
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.4)

<400>  20
caagtccaat tggtccagag tggagctgaa gttgttaaac caggagcaag cgttaaagtt       60

agttgtaagg catcaggtta cgccttcacc aactacttga ttgaatgggt aaagcaacgt      120

cccggtcagg gccttgagtg gattggggtt ataaatcctg gttctggggg aactaattac      180

aacgagaagt tcaagggcaa ggccaccctt actgccgaca agtccataag caccgcctat      240

atggaattga gcagattgcg aagtgatgac accgccgtct actattgtgc ttcaggtaat      300

tatgatacct attgggggca aggaacactg gtgaccgtat cttcc                      345


<210>  21
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.5)

<400>  21
caagtccaac tggttcagtc tggagctgaa gtaaaaaagc ctggggccag tgtgaaagta       60

tcctgcaaag ccagcggcta cgcatttact aattatctga ttgaatgggt caagcaggca      120

ccaggacagg gtctggaatg gattggggtt ataaatccag gaagtggggg gactaattac      180

aatgagaaat ttaaaggccg agcaaccttg acacgcgata cctcaataag taccgcatat      240

atggaactca gtcgtctgcg aagtgatgat accgctgttt atttctgtgc tagtggcaac      300

tatgacacat actggggaca gggtacattg gtaacagtga gtagc                      345


<210>  22
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.6)

<400>  22
caagtgcagc tggtacagtc cggagctgaa gtcaagaaac caggggcctc agtgaaagtt       60

tcatgtaagg cttctggata tgcctttacc aactacctta tcgagtgggt taaacaggcc      120

ccagggcaag gattggagtg gatcggggtc atcaatccag gatcaggtgg tacaaactat      180

aatgagaaat tcaagggtag agctactctg acagccgata caagtatcag cactgcatat      240

atggagttga gccgtctccg cagcgacgac acagccgtat atttctgtgc atctggcaat      300

tatgacacat actggggcca gggaactctt gtcaccgtct cctca                      345


<210>  23
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.7)

<400>  23
caggtccaat tggtgcaatc aggggccgag gtcaaaaaac ctggagcaag tgttaaagtg       60

agctgtaagg cctctggcta cgcctttaca aattatctca tcgaatgggt aaagcaagca      120

ccagggcaag gactcgaatg gatgggcgtg attaacccag gatcaggggg aaccaattac      180

aacgaaaagt ttaaggggaa ggctacactg actgctgata aatccatatc tactgcatac      240

atggaactga gccggctccg ttctgacgac actgctgtct attattgcgc ttctggcaat      300

tatgatactt attgggggca aggcactctg gttacagtca gctcc                      345


<210>  24
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.8)

<400>  24
caagtccagc tcgtacaaag tggtgccgag gttaagaaac ctggtgctag tgtgaaagtc       60

tcatgcaagg ctagtggcta cacttttacc aattacctga ttgaatgggt ccgacaagcc      120

ccaggtcagg gattggagtg gatgggagtc attaaccctg gctcaggtgg cactaattat      180

aacgaaaagt ttaaaggccg tgtgactatg accgccgaca agagcatctc tactgcatac      240

atggaactca gccgtctgcg ttctgatgat actgctgtgt attattgtgc atctgggaac      300

tacgacactt attggggaca gggcactctt gtaaccgtat cctct                      345


<210>  25
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (ver.9)

<400>  25
caagtgcagt tggtgcaaag tggcgctgaa gttaagaagc ctggtgcttc agtcaaagta       60

tcttgcaaag ctagtggtta cacctttacc aactacctga tcgaatgggt caggcaggca      120

cccggacaag gtttggaatg gattggagtc ataaaccccg gctccggagg aactaactac      180

aacgagaaat tcaaaggcag ggccactctc accgctgata aaagtatttc aactgcatat      240

atggaacttt ctaggcttcg gtcagatgac actgctgtct actattgtgc atccggcaat      300

tacgatactt attggggcca ggggacattg gtcacagtgt cttct                      345


<210>  26
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ch1E4 (CPF)_VH

<400>  26
caagtccaac tccaacaaag tggagcagag ttggtgcgcc caggcacaag tgtcaaagtt       60

agctgtaagg cttcaggcta tgcatttaca aattacttga ttgagtgggt gaaacagcga      120

cccggacaag gcttggaatg gatcggtgtc atcaatccag gatctggggg aacaaattat      180

aacgaaaaat tcaaggggaa agccacactc accgccgata aaagttcatc caccgcatat      240

atgcagctta gctctctcac atctgacgat agcgcagtgt atttctgcgc atccggtaat      300

tacgatactt attggggcca aggtactctg gtgaccgtta gcgcc                      345


<210>  27
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (VL1)

<400>  27
gacattgtga tgactcagac tccccttagt cttagtgtta cccccgggca accagcatca       60

atatcatgcc gcagctcaca gagtctggtt cactctaatg gcaacactta tcttcactgg      120

tatctccaga aacccggtca atctccacaa ctgttgatct acaaagtgtc caatcgcttt      180

tcaggcgtgc ccgaccgatt tagtggtagc gggtcaggga ccgactttac tctgaaaatt      240

tcaagagtag aggctgaaga tgtaggagtc tactactgct ctcaaagtac ccatgttccc      300

agaacattcg gccagggcac caaactggag attaag                                336


<210>  28
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (VL2)

<400>  28
gatgtagtga tgacacaaac ccctttgtca ttgagcgtca cccccggaca gcccgcttcc       60

attagttgcc gaagctcaca gagtctcgtc cactccaacg gtaacacata cctccactgg      120

tacttgcaga agcctggaca gagcccccaa ttgctgatat ataaagtgtc aaacagattt      180

agcggggtgc ccgatagatt ctccggtagc ggctcaggca ccgactttac attgaagatc      240

agtcgcgtcg aagcagagga tgttggcgtg tattattgca gtcaaagcac acatgttcct      300

aggacttttg ggcagggtac taagttggaa ataaag                                336


<210>  29
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (VL3)

<400>  29
gatgtagtaa tgactcaatc tccacttagc ctccccgtga cactcggcca accagcaagc       60

atatcctgtc gttccagcca gtccctcgtg cattctaacg gcaacaccta cttgcattgg      120

ttccaacagc gtccaggaca gagtcctcgg cgtttgatat ataaggtgtc caaccgattc      180

agcggggttc ctgataggtt ttctggatct ggttcaggca ccgatttcac tttgaaaata      240

tctcgcgttg aggccgaaga cgtaggtgtg tattactgtt cccagtccac ccatgtgcct      300

cgaaccttcg ggggaggtac aaaagtggaa atcaaa                                336


<210>  30
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (VL4)

<400>  30
gatgtggtaa tgacacagtc ccctctctca ttgcccgtga cactggggca gcccgcttcc       60

attagctgtc gaagtagtca gagcttggtt catagcaatg gtaatacata tttgcactgg      120

tatcaacaac gtccaggcca atcaccacga ctccttattt acaaagtctc taaccggttt      180

agcggtgtac cagaccgttt ctctggttca gggagtggga ccgatttcac tcttaaaatt      240

agtcgggtcg aagccgaaga cgttggcgtc tactattgca gccagtcaac tcatgtacca      300

cgaactttcg gcggcggtac caaggttgag attaag                                336


<210>  31
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 (VL5)

<400>  31
gatgttgtga tgacacagtc cccactctct ctgcccgtca cattgggtca acccgcaagc       60

atctcctgtc gttccagcca gagtcttgta cattccaatg gcaatactta cctccattgg      120

taccaacaga gacccggtca aagccctcgt ctcttgatat ataaggtttc aaacaggttc      180

tcaggggtac ccgaccgatt cagcggttca gggtccggga cagacttcac cctcaaaatt      240

agtcgtgtgg aggctgagga cgtgggtgta tatttctgta gccagagtac ccatgtacct      300

cgcacctttg ggggaggcac caaggttgaa attaaa                                336


<210>  32
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: ch1E4 (CPF)_VL

<400>  32
gacgttgtaa tgactcaaac accactttct ttgccagtat cacttggaga tcaagcttct       60

atctcatgta ggagctccca gtcactggtg cattcaaacg gaaacacata ccttcactgg      120

tatctgcaaa aaccaggcca atcaccaaag cttttgatat acaaagtgtc aaatcgtttt      180

agcggagtac cagaccgatt cagcgggagc ggaagcggga ctgacttcac acttaagatt      240

agcagagtgg aagccgaaga cctgggtgtc tatttttgca gccagagcac ccatgtcccc      300

cgcacattcg gaggtggaac caagctggag ataaaa                                336


<210>  33
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: HCDR1

<400>  33

Asn Tyr Leu Ile Glu 
1               5   


<210>  34
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: HCDR2

<400>  34

Val Ile Asn Pro Gly Ser Gly Gly Thr Asn Tyr Asn Glu Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  35
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: HCDR3

<400>  35

Gly Asn Tyr Asp Thr Tyr 
1               5       


<210>  36
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: LCDR1

<400>  36

Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His 
1               5                   10                  15      


<210>  37
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: LCDR2

<400>  37

Lys Val Ser Asn Arg Phe Ser 
1               5           


<210>  38
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: LCDR3

<400>  38

Ser Gln Ser Thr His Val Pro Arg Thr 
1               5                   


<210>  39
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: 1E4 hIgG1 heavy chain constant region (knob)

<400>  39

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  40
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: 1E4 hIgG1 heavy chain constant region (hole)

<400>  40

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Val Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  41
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: 1E4 hIgG1 heavy chain constant region (knob) (M428L)

<400>  41

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Leu His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  42
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: 1E4 hIgG1 heavy chain constant region (hole) (M428L)

<400>  42

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Val Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Leu His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  43
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: 1E4 hIgG1 light chain (human kappa) constant region

<400>  43

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  44
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: (G4S)3 Linker -  linker between 1E4 hIgG1 and Grabody 
       B scFv

<400>  44

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  45
<211>  110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Gra-body B anti-IGF1R scFv, VL

<400>  45

Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln 
1               5                   10                  15      


Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn 
            20                  25                  30          


Asp Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Ala Asn Val Asn Arg Pro Ser Gly Val Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Arg 
65                  70                  75                  80  


Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Gly Thr Trp Ala Gly Ser Leu 
                85                  90                  95      


Asn Ala Tyr Val Phe Gly Cys Gly Thr Lys Leu Thr Val Leu 
            100                 105                 110 


<210>  46
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Gra-body B anti-IGF1R scFv, LCDR1

<400>  46

Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn Asp Val Ser 
1               5                   10              


<210>  47
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Gra-body B anti-IGF1R scFv, LCDR2

<400>  47

Ala Asn Val Asn Arg Pro Ser 
1               5           


<210>  48
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Gra-body B anti-IGF1R scFv, LCDR3

<400>  48

Gly Thr Trp Ala Gly Ser Leu Asn Ala Tyr Val 
1               5                   10      


<210>  49
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: (G4S)4 Linker between VL and VH

<400>  49

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  50
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Gra-body B anti-IGF1R scFv, VH

<400>  50

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Tyr Asp Asn Ala Asn Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Val Leu Thr Thr Leu Met Asn Trp Phe Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120     


<210>  51
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Gra-body B anti-IGF1R scFv, HCDR1

<400>  51

Gly Phe Thr Phe Ser Ser Tyr Asp Met Ser 
1               5                   10  


<210>  52
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Gra-body B anti-IGF1R scFv, HCDR2

<400>  52

Ala Ile Ser Tyr Asp Asn Ala Asn Thr Tyr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  53
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Gra-body B anti-IGF1R scFv, HCDR3

<400>  53

Gly Val Leu Thr Thr Leu Met Asn Trp Phe Asp Tyr 
1               5                   10          


<210>  54
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Gra-body B anti-IGF1R scFv, Full scFv sequence

<400>  54

Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln 
1               5                   10                  15      


Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn 
            20                  25                  30          


Asp Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Ala Asn Val Asn Arg Pro Ser Gly Val Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Arg 
65                  70                  75                  80  


Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Gly Thr Trp Ala Gly Ser Leu 
                85                  90                  95      


Asn Ala Tyr Val Phe Gly Cys Gly Thr Lys Leu Thr Val Leu Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro 
    130                 135                 140                 


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser 
145                 150                 155                 160 


Ser Tyr Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Cys Leu Glu 
                165                 170                 175     


Trp Val Ser Ala Ile Ser Tyr Asp Asn Ala Asn Thr Tyr Tyr Ala Asp 
            180                 185                 190         


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
        195                 200                 205             


Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
    210                 215                 220                 


Tyr Cys Ala Lys Gly Val Leu Thr Thr Leu Met Asn Trp Phe Asp Tyr 
225                 230                 235                 240 


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
                245                 250     


<210>  55
<211>  596
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hIgG1- Grabody B constant region, incl. Grabody B scFv
       (hole)

<400>  55

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Val Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Gly Gly Gly Gly Ser Gly 
                325                 330                 335     


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ser Val Leu Thr Gln Pro 
            340                 345                 350         


Pro Ser Ala Ser Gly Thr Pro Gly Gln Arg Val Thr Ile Ser Cys Thr 
        355                 360                 365             


Gly Ser Ser Ser Asn Ile Gly Ser Asn Asp Val Ser Trp Tyr Gln Gln 
    370                 375                 380                 


Leu Pro Gly Thr Ala Pro Lys Leu Leu Ile Tyr Ala Asn Val Asn Arg 
385                 390                 395                 400 


Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser 
                405                 410                 415     


Ala Ser Leu Ala Ile Ser Gly Leu Arg Ser Glu Asp Glu Ala Asp Tyr 
            420                 425                 430         


Tyr Cys Gly Thr Trp Ala Gly Ser Leu Asn Ala Tyr Val Phe Gly Cys 
        435                 440                 445             


Gly Thr Lys Leu Thr Val Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    450                 455                 460                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Leu 
465                 470                 475                 480 


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser 
                485                 490                 495     


Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Asp Met Ser Trp Val 
            500                 505                 510         


Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Val Ser Ala Ile Ser Tyr 
        515                 520                 525             


Asp Asn Ala Asn Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr 
    530                 535                 540                 


Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser 
545                 550                 555                 560 


Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys Gly Val Leu 
                565                 570                 575     


Thr Thr Leu Met Asn Trp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            580                 585                 590         


Thr Val Ser Ser 
        595     


<210>  56
<211>  596
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hIgG1 (M428L)-Grabody B constant region, incl. Grabody
       B scFv (hole)

<400>  56

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Val Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Leu His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Gly Gly Gly Gly Ser Gly 
                325                 330                 335     


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ser Val Leu Thr Gln Pro 
            340                 345                 350         


Pro Ser Ala Ser Gly Thr Pro Gly Gln Arg Val Thr Ile Ser Cys Thr 
        355                 360                 365             


Gly Ser Ser Ser Asn Ile Gly Ser Asn Asp Val Ser Trp Tyr Gln Gln 
    370                 375                 380                 


Leu Pro Gly Thr Ala Pro Lys Leu Leu Ile Tyr Ala Asn Val Asn Arg 
385                 390                 395                 400 


Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser 
                405                 410                 415     


Ala Ser Leu Ala Ile Ser Gly Leu Arg Ser Glu Asp Glu Ala Asp Tyr 
            420                 425                 430         


Tyr Cys Gly Thr Trp Ala Gly Ser Leu Asn Ala Tyr Val Phe Gly Cys 
        435                 440                 445             


Gly Thr Lys Leu Thr Val Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    450                 455                 460                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Leu 
465                 470                 475                 480 


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser 
                485                 490                 495     


Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Asp Met Ser Trp Val 
            500                 505                 510         


Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Val Ser Ala Ile Ser Tyr 
        515                 520                 525             


Asp Asn Ala Asn Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr 
    530                 535                 540                 


Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser 
545                 550                 555                 560 


Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys Gly Val Leu 
                565                 570                 575     


Thr Thr Leu Met Asn Trp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            580                 585                 590         


Thr Val Ser Ser 
        595     


<210>  57
<211>  711
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 IgG1 (V1_VL1) x Grabody B (M428L) heavy chain 
       with Grabody B scFv (hole)

<400>  57

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Leu Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Val Ile Asn Pro Gly Ser Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Gly Asn Tyr Asp Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
        115                 120                 125             


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
    130                 135                 140                 


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
145                 150                 155                 160 


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
                165                 170                 175     


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
            180                 185                 190         


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
        195                 200                 205             


Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Val Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Gln Gly Asn Val Phe Ser Cys Ser Val Leu His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Gly Gly Gly 
        435                 440                 445             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ser Val Leu 
    450                 455                 460                 


Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln Arg Val Thr Ile 
465                 470                 475                 480 


Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn Asp Val Ser Trp 
                485                 490                 495     


Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu Ile Tyr Ala Asn 
            500                 505                 510         


Val Asn Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Lys Ser 
        515                 520                 525             


Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Arg Ser Glu Asp Glu 
    530                 535                 540                 


Ala Asp Tyr Tyr Cys Gly Thr Trp Ala Gly Ser Leu Asn Ala Tyr Val 
545                 550                 555                 560 


Phe Gly Cys Gly Thr Lys Leu Thr Val Leu Gly Gly Gly Gly Ser Gly 
                565                 570                 575     


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val 
            580                 585                 590         


Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu 
        595                 600                 605             


Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Asp Met 
    610                 615                 620                 


Ser Trp Val Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Val Ser Ala 
625                 630                 635                 640 


Ile Ser Tyr Asp Asn Ala Asn Thr Tyr Tyr Ala Asp Ser Val Lys Gly 
                645                 650                 655     


Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln 
            660                 665                 670         


Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys 
        675                 680                 685             


Gly Val Leu Thr Thr Leu Met Asn Trp Phe Asp Tyr Trp Gly Gln Gly 
    690                 695                 700                 


Thr Leu Val Thr Val Ser Ser 
705                 710     


<210>  58
<211>  445
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: hu1E4 IgG1 (V1_VL1) x Grabody B (M428L) heavy chain 
       without Grabody B (knob)

<400>  58

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Leu Ile Glu Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Val Ile Asn Pro Gly Ser Gly Gly Thr Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Gly Asn Tyr Asp Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
        115                 120                 125             


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
    130                 135                 140                 


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
145                 150                 155                 160 


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
                165                 170                 175     


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
            180                 185                 190         


Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys 
        195                 200                 205             


Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Gln Gly Asn Val Phe Ser Cys Ser Val Leu His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445 


<210>  59
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Light Chain

<400>  59

Asp Ile Val Met Thr Gln Thr Pro Leu Ser Leu Ser Val Thr Pro Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Arg Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


<210>  60
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Homo sapiens alpha-synuclein

<400>  60

Met Asp Val Phe Met Lys Gly Leu Ser Lys Ala Lys Glu Gly Val Val 
1               5                   10                  15      


Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Ala Glu Ala Ala Gly Lys 
            20                  25                  30          


Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Lys Glu Gly Val 
        35                  40                  45              


Val His Gly Val Ala Thr Val Ala Glu Lys Thr Lys Glu Gln Val Thr 
    50                  55                  60                  


Asn Val Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys 
65                  70                  75                  80  


Thr Val Glu Gly Ala Gly Ser Ile Ala Ala Ala Thr Gly Phe Val Lys 
                85                  90                  95      


Lys Asp Gln Leu Gly Lys Asn Glu Glu Gly Ala Pro Gln Glu Gly Ile 
            100                 105                 110         


Leu Glu Asp Met Pro Val Asp Pro Asp Asn Glu Ala Tyr Glu Met Pro 
        115                 120                 125             


Ser Glu Glu Gly Tyr Gln Asp Tyr Glu Pro Glu Ala 
    130                 135                 140 


<210>  61
<211>  30
<212>  PRT
<213>  Homo sapiens


<220>
<221>  SITE
<222>  (1)..(30)
<223>  Human VH1-02 FR1

<400>  61

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
            20                  25                  30  


<210>  62
<211>  14
<212>  PRT
<213>  Homo sapiens


<220>
<221>  SITE
<222>  (1)..(14)
<223>  Human VH1-02 FR2

<400>  62

Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met Gly 
1               5                   10                  


<210>  63
<211>  32
<212>  PRT
<213>  Homo sapiens


<220>
<221>  SITE
<222>  (1)..(32)
<223>  Human VH1-02 FR3

<400>  63

Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu 
1               5                   10                  15      


Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
            20                  25                  30          


<210>  64
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu1E4V1 HCDR1 (IMGT)

<400>  64

Gly Tyr Thr Phe Thr Asn Tyr Leu 
1               5               


<210>  65
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu1E4V1 HCDR2 (IMGT)

<400>  65

Ile Asn Pro Gly Ser Gly Gly Thr 
1               5               


<210>  66
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu1E4V1 HCDR3 (IMGT)

<400>  66

Ala Ser Gly Asn Tyr Asp Thr Tyr 
1               5               


<210>  67
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu1E4VL1 LCDR1 (IMGT)

<400>  67

Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr 
1               5                   10      


<210>  68
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu1E4VL1 LCDR2 (IMGT)

<400>  68

Lys Val Ser 
1           


<210>  69
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hu1E4VL1 LCDR3 (IMGT)

<400>  69

Ser Gln Ser Thr His Val Pro Arg Thr 
1               5                   


<210>  70
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Peptide linker


<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  repeats n times where n is an integer of at least 1

<400>  70

Gly Ser Gly Gly Ser 
1               5   


<210>  71
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Peptide linker


<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  repeats n times where n is an integer of at least 1

<400>  71

Gly Gly Gly Gly Ser 
1               5   


<210>  72
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Peptide linker


<220>
<221>  REPEAT
<222>  (1)..(4)
<223>  repeats n times where n is an integer of at least 1

<400>  72

Gly Gly Gly Ser 
1               


<210>  73
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Peptide linker

<400>  73

Ala Ala Glu Pro Lys Ser Ser 
1               5           


<210>  74
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Peptide linker

<400>  74

Ala Ala Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys 
1               5                   10                  15      


Pro 
    


<210>  75
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Peptide linker

<400>  75

Gly Gly Gly Gly 
1               


<210>  76
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Peptide linker

<400>  76

Gly Gly Gly Gly Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  


<210>  77
<211>  1367
<212>  PRT
<213>  Homo sapiens


<220>
<221>  SITE
<222>  (1)..(1367)
<223>  Human IGF1R

<400>  77

Met Lys Ser Gly Ser Gly Gly Gly Ser Pro Thr Ser Leu Trp Gly Leu 
1               5                   10                  15      


Leu Phe Leu Ser Ala Ala Leu Ser Leu Trp Pro Thr Ser Gly Glu Ile 
            20                  25                  30          


Cys Gly Pro Gly Ile Asp Ile Arg Asn Asp Tyr Gln Gln Leu Lys Arg 
        35                  40                  45              


Leu Glu Asn Cys Thr Val Ile Glu Gly Tyr Leu His Ile Leu Leu Ile 
    50                  55                  60                  


Ser Lys Ala Glu Asp Tyr Arg Ser Tyr Arg Phe Pro Lys Leu Thr Val 
65                  70                  75                  80  


Ile Thr Glu Tyr Leu Leu Leu Phe Arg Val Ala Gly Leu Glu Ser Leu 
                85                  90                  95      


Gly Asp Leu Phe Pro Asn Leu Thr Val Ile Arg Gly Trp Lys Leu Phe 
            100                 105                 110         


Tyr Asn Tyr Ala Leu Val Ile Phe Glu Met Thr Asn Leu Lys Asp Ile 
        115                 120                 125             


Gly Leu Tyr Asn Leu Arg Asn Ile Thr Arg Gly Ala Ile Arg Ile Glu 
    130                 135                 140                 


Lys Asn Ala Asp Leu Cys Tyr Leu Ser Thr Val Asp Trp Ser Leu Ile 
145                 150                 155                 160 


Leu Asp Ala Val Ser Asn Asn Tyr Ile Val Gly Asn Lys Pro Pro Lys 
                165                 170                 175     


Glu Cys Gly Asp Leu Cys Pro Gly Thr Met Glu Glu Lys Pro Met Cys 
            180                 185                 190         


Glu Lys Thr Thr Ile Asn Asn Glu Tyr Asn Tyr Arg Cys Trp Thr Thr 
        195                 200                 205             


Asn Arg Cys Gln Lys Met Cys Pro Ser Thr Cys Gly Lys Arg Ala Cys 
    210                 215                 220                 


Thr Glu Asn Asn Glu Cys Cys His Pro Glu Cys Leu Gly Ser Cys Ser 
225                 230                 235                 240 


Ala Pro Asp Asn Asp Thr Ala Cys Val Ala Cys Arg His Tyr Tyr Tyr 
                245                 250                 255     


Ala Gly Val Cys Val Pro Ala Cys Pro Pro Asn Thr Tyr Arg Phe Glu 
            260                 265                 270         


Gly Trp Arg Cys Val Asp Arg Asp Phe Cys Ala Asn Ile Leu Ser Ala 
        275                 280                 285             


Glu Ser Ser Asp Ser Glu Gly Phe Val Ile His Asp Gly Glu Cys Met 
    290                 295                 300                 


Gln Glu Cys Pro Ser Gly Phe Ile Arg Asn Gly Ser Gln Ser Met Tyr 
305                 310                 315                 320 


Cys Ile Pro Cys Glu Gly Pro Cys Pro Lys Val Cys Glu Glu Glu Lys 
                325                 330                 335     


Lys Thr Lys Thr Ile Asp Ser Val Thr Ser Ala Gln Met Leu Gln Gly 
            340                 345                 350         


Cys Thr Ile Phe Lys Gly Asn Leu Leu Ile Asn Ile Arg Arg Gly Asn 
        355                 360                 365             


Asn Ile Ala Ser Glu Leu Glu Asn Phe Met Gly Leu Ile Glu Val Val 
    370                 375                 380                 


Thr Gly Tyr Val Lys Ile Arg His Ser His Ala Leu Val Ser Leu Ser 
385                 390                 395                 400 


Phe Leu Lys Asn Leu Arg Leu Ile Leu Gly Glu Glu Gln Leu Glu Gly 
                405                 410                 415     


Asn Tyr Ser Phe Tyr Val Leu Asp Asn Gln Asn Leu Gln Gln Leu Trp 
            420                 425                 430         


Asp Trp Asp His Arg Asn Leu Thr Ile Lys Ala Gly Lys Met Tyr Phe 
        435                 440                 445             


Ala Phe Asn Pro Lys Leu Cys Val Ser Glu Ile Tyr Arg Met Glu Glu 
    450                 455                 460                 


Val Thr Gly Thr Lys Gly Arg Gln Ser Lys Gly Asp Ile Asn Thr Arg 
465                 470                 475                 480 


Asn Asn Gly Glu Arg Ala Ser Cys Glu Ser Asp Val Leu His Phe Thr 
                485                 490                 495     


Ser Thr Thr Thr Ser Lys Asn Arg Ile Ile Ile Thr Trp His Arg Tyr 
            500                 505                 510         


Arg Pro Pro Asp Tyr Arg Asp Leu Ile Ser Phe Thr Val Tyr Tyr Lys 
        515                 520                 525             


Glu Ala Pro Phe Lys Asn Val Thr Glu Tyr Asp Gly Gln Asp Ala Cys 
    530                 535                 540                 


Gly Ser Asn Ser Trp Asn Met Val Asp Val Asp Leu Pro Pro Asn Lys 
545                 550                 555                 560 


Asp Val Glu Pro Gly Ile Leu Leu His Gly Leu Lys Pro Trp Thr Gln 
                565                 570                 575     


Tyr Ala Val Tyr Val Lys Ala Val Thr Leu Thr Met Val Glu Asn Asp 
            580                 585                 590         


His Ile Arg Gly Ala Lys Ser Glu Ile Leu Tyr Ile Arg Thr Asn Ala 
        595                 600                 605             


Ser Val Pro Ser Ile Pro Leu Asp Val Leu Ser Ala Ser Asn Ser Ser 
    610                 615                 620                 


Ser Gln Leu Ile Val Lys Trp Asn Pro Pro Ser Leu Pro Asn Gly Asn 
625                 630                 635                 640 


Leu Ser Tyr Tyr Ile Val Arg Trp Gln Arg Gln Pro Gln Asp Gly Tyr 
                645                 650                 655     


Leu Tyr Arg His Asn Tyr Cys Ser Lys Asp Lys Ile Pro Ile Arg Lys 
            660                 665                 670         


Tyr Ala Asp Gly Thr Ile Asp Ile Glu Glu Val Thr Glu Asn Pro Lys 
        675                 680                 685             


Thr Glu Val Cys Gly Gly Glu Lys Gly Pro Cys Cys Ala Cys Pro Lys 
    690                 695                 700                 


Thr Glu Ala Glu Lys Gln Ala Glu Lys Glu Glu Ala Glu Tyr Arg Lys 
705                 710                 715                 720 


Val Phe Glu Asn Phe Leu His Asn Ser Ile Phe Val Pro Arg Pro Glu 
                725                 730                 735     


Arg Lys Arg Arg Asp Val Met Gln Val Ala Asn Thr Thr Met Ser Ser 
            740                 745                 750         


Arg Ser Arg Asn Thr Thr Ala Ala Asp Thr Tyr Asn Ile Thr Asp Pro 
        755                 760                 765             


Glu Glu Leu Glu Thr Glu Tyr Pro Phe Phe Glu Ser Arg Val Asp Asn 
    770                 775                 780                 


Lys Glu Arg Thr Val Ile Ser Asn Leu Arg Pro Phe Thr Leu Tyr Arg 
785                 790                 795                 800 


Ile Asp Ile His Ser Cys Asn His Glu Ala Glu Lys Leu Gly Cys Ser 
                805                 810                 815     


Ala Ser Asn Phe Val Phe Ala Arg Thr Met Pro Ala Glu Gly Ala Asp 
            820                 825                 830         


Asp Ile Pro Gly Pro Val Thr Trp Glu Pro Arg Pro Glu Asn Ser Ile 
        835                 840                 845             


Phe Leu Lys Trp Pro Glu Pro Glu Asn Pro Asn Gly Leu Ile Leu Met 
    850                 855                 860                 


Tyr Glu Ile Lys Tyr Gly Ser Gln Val Glu Asp Gln Arg Glu Cys Val 
865                 870                 875                 880 


Ser Arg Gln Glu Tyr Arg Lys Tyr Gly Gly Ala Lys Leu Asn Arg Leu 
                885                 890                 895     


Asn Pro Gly Asn Tyr Thr Ala Arg Ile Gln Ala Thr Ser Leu Ser Gly 
            900                 905                 910         


Asn Gly Ser Trp Thr Asp Pro Val Phe Phe Tyr Val Gln Ala Lys Thr 
        915                 920                 925             


Gly Tyr Glu Asn Phe Ile His Leu Ile Ile Ala Leu Pro Val Ala Val 
    930                 935                 940                 


Leu Leu Ile Val Gly Gly Leu Val Ile Met Leu Tyr Val Phe His Arg 
945                 950                 955                 960 


Lys Arg Asn Asn Ser Arg Leu Gly Asn Gly Val Leu Tyr Ala Ser Val 
                965                 970                 975     


Asn Pro Glu Tyr Phe Ser Ala Ala Asp Val Tyr Val Pro Asp Glu Trp 
            980                 985                 990         


Glu Val Ala Arg Glu Lys Ile Thr  Met Ser Arg Glu Leu  Gly Gln Gly 
        995                 1000                 1005             


Ser Phe  Gly Met Val Tyr Glu  Gly Val Ala Lys Gly  Val Val Lys 
    1010                 1015                 1020             


Asp Glu  Pro Glu Thr Arg Val  Ala Ile Lys Thr Val  Asn Glu Ala 
    1025                 1030                 1035             


Ala Ser  Met Arg Glu Arg Ile  Glu Phe Leu Asn Glu  Ala Ser Val 
    1040                 1045                 1050             


Met Lys  Glu Phe Asn Cys His  His Val Val Arg Leu  Leu Gly Val 
    1055                 1060                 1065             


Val Ser  Gln Gly Gln Pro Thr  Leu Val Ile Met Glu  Leu Met Thr 
    1070                 1075                 1080             


Arg Gly  Asp Leu Lys Ser Tyr  Leu Arg Ser Leu Arg  Pro Glu Met 
    1085                 1090                 1095             


Glu Asn  Asn Pro Val Leu Ala  Pro Pro Ser Leu Ser  Lys Met Ile 
    1100                 1105                 1110             


Gln Met  Ala Gly Glu Ile Ala  Asp Gly Met Ala Tyr  Leu Asn Ala 
    1115                 1120                 1125             


Asn Lys  Phe Val His Arg Asp  Leu Ala Ala Arg Asn  Cys Met Val 
    1130                 1135                 1140             


Ala Glu  Asp Phe Thr Val Lys  Ile Gly Asp Phe Gly  Met Thr Arg 
    1145                 1150                 1155             


Asp Ile  Tyr Glu Thr Asp Tyr  Tyr Arg Lys Gly Gly  Lys Gly Leu 
    1160                 1165                 1170             


Leu Pro  Val Arg Trp Met Ser  Pro Glu Ser Leu Lys  Asp Gly Val 
    1175                 1180                 1185             


Phe Thr  Thr Tyr Ser Asp Val  Trp Ser Phe Gly Val  Val Leu Trp 
    1190                 1195                 1200             


Glu Ile  Ala Thr Leu Ala Glu  Gln Pro Tyr Gln Gly  Leu Ser Asn 
    1205                 1210                 1215             


Glu Gln  Val Leu Arg Phe Val  Met Glu Gly Gly Leu  Leu Asp Lys 
    1220                 1225                 1230             


Pro Asp  Asn Cys Pro Asp Met  Leu Phe Glu Leu Met  Arg Met Cys 
    1235                 1240                 1245             


Trp Gln  Tyr Asn Pro Lys Met  Arg Pro Ser Phe Leu  Glu Ile Ile 
    1250                 1255                 1260             


Ser Ser  Ile Lys Glu Glu Met  Glu Pro Gly Phe Arg  Glu Val Ser 
    1265                 1270                 1275             


Phe Tyr  Tyr Ser Glu Glu Asn  Lys Leu Pro Glu Pro  Glu Glu Leu 
    1280                 1285                 1290             


Asp Leu  Glu Pro Glu Asn Met  Glu Ser Val Pro Leu  Asp Pro Ser 
    1295                 1300                 1305             


Ala Ser  Ser Ser Ser Leu Pro  Leu Pro Asp Arg His  Ser Gly His 
    1310                 1315                 1320             


Lys Ala  Glu Asn Gly Pro Gly  Pro Gly Val Leu Val  Leu Arg Ala 
    1325                 1330                 1335             


Ser Phe  Asp Glu Arg Gln Pro  Tyr Ala His Met Asn  Gly Gly Arg 
    1340                 1345                 1350             


Lys Asn  Glu Arg Ala Leu Pro  Leu Pro Gln Ser Ser  Thr Cys 
    1355                 1360                 1365         


