<110>    MediMabBio Inc.

<120>    Anti-TIGIT antibodies and use thereof

<130>    OPP20221056KR

<150>    10-2021-0060014
<151>    2021-05-10

<160>    31

<170>    koPatentIn 3.0

<210>    1
<211>    6
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_CDR-H1


<400>    1
Ser Asp Tyr Ala Trp Asn
  1               5    


<210>    2
<211>    16
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_CDR-H2


<400>    2
Tyr Ile Ser Tyr Ser Gly Ser Ala Arg Tyr Asn Pro Ser Leu Lys Ser
  1               5                  10                  15 




<210>    3
<211>    9
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_CDR-H3


<400>    3
Lys Gly Tyr Pro Ala Tyr Phe Ala Tyr
  1               5                


<210>    4
<211>    11
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_CDR-L1, wherein X is K or R


<400>    4
Xaa Ala Ser Gln Asp Val Ser Thr Ala Val Ala
  1               5                  10    


<210>    5
<211>    7
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_CDR-L2


<400>    5
Ser Ala Ser Tyr Arg Tyr Thr
  1               5        


<210>    6
<211>    9
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_CDR-L3


<400>    6
Gln His His Tyr Ser Thr Pro Tyr Thr
  1               5                


<210>    7
<211>    11
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_CDR-L1


<400>    7
Lys Ala Ser Gln Asp Val Ser Thr Ala Val Ala
  1               5                  10    


<210>    8
<211>    11
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_CDR-L1


<400>    8
Arg Ala Ser Gln Asp Val Ser Thr Ala Val Ala
  1               5                  10    


<210>    9
<211>    118
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Heavy chain variable region


<400>    9
Asp Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln
  1               5                  10                  15 

Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp
             20                  25                  30 

Tyr Ala Trp Asn Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp
         35                  40                  45 

Met Gly Tyr Ile Ser Tyr Ser Gly Ser Ala Arg Tyr Asn Pro Ser Leu
     50                  55                  60 

Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Met Asn Gln Phe Phe
 65                  70                  75                  80 

Leu Gln Leu Asn Ser Val Thr Ala Glu Asp Thr Ala Thr Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Lys Gly Tyr Pro Ala Tyr Phe Ala Tyr Trp Gly Gln Gly Thr
            100                 105                 110 

Leu Val Thr Val Ser Ser
        115            


<210>    10
<211>    118
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Heavy chain variable region


<400>    10
Asp Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln
  1               5                  10                  15 

Thr Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp
             20                  25                  30 

Tyr Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp
         35                  40                  45 

Met Gly Tyr Ile Ser Tyr Ser Gly Ser Ala Arg Tyr Asn Pro Ser Leu
     50                  55                  60 

Lys Ser Arg Ile Thr Ile Ser Arg Asp Thr Ser Met Asn Gln Phe Ser
 65                  70                  75                  80 

Leu Lys Leu Asn Ser Val Thr Ala Glu Asp Thr Ala Thr Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Lys Gly Tyr Pro Ala Tyr Phe Ala Tyr Trp Gly Gln Gly Thr
            100                 105                 110 

Leu Val Thr Val Ser Ser
        115            


<210>    11
<211>    118
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Heavy chain variable region


<400>    11
Asp Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln
  1               5                  10                  15 

Thr Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp
             20                  25                  30 

Tyr Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp
         35                  40                  45 

Met Gly Tyr Ile Ser Tyr Ser Gly Ser Ala Arg Tyr Asn Pro Ser Leu
     50                  55                  60 

Lys Ser Arg Ile Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Phe Ser
 65                  70                  75                  80 

Leu Lys Leu Ser Ser Val Thr Ala Glu Asp Thr Ala Thr Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Lys Gly Tyr Pro Ala Tyr Phe Ala Tyr Trp Gly Gln Gly Thr
            100                 105                 110 

Leu Val Thr Val Ser Ser
        115            


<210>    12
<211>    118
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Heavy chain variable region


<400>    12
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln
  1               5                  10                  15 

Thr Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp
             20                  25                  30 

Tyr Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp
         35                  40                  45 

Met Gly Tyr Ile Ser Tyr Ser Gly Ser Ala Arg Tyr Asn Pro Ser Leu
     50                  55                  60 

Lys Ser Arg Val Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Phe Ser
 65                  70                  75                  80 

Leu Lys Leu Ser Ser Val Thr Ala Glu Asp Thr Ala Thr Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Lys Gly Tyr Pro Ala Tyr Phe Ala Tyr Trp Gly Gln Gly Thr
            100                 105                 110 

Leu Val Thr Val Ser Ser
        115            


<210>    13
<211>    118
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Heavy chain variable region


<400>    13
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln
  1               5                  10                  15 

Thr Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp
             20                  25                  30 

Tyr Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp
         35                  40                  45 

Met Gly Tyr Ile Ser Tyr Ser Gly Ser Ala Arg Tyr Asn Pro Ser Leu
     50                  55                  60 

Lys Ser Arg Val Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Phe Ser
 65                  70                  75                  80 

Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Lys Gly Tyr Pro Ala Tyr Phe Ala Tyr Trp Gly Gln Gly Thr
            100                 105                 110 

Leu Val Thr Val Ser Ser
        115            


<210>    14
<211>    118
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Heavy chain variable region


<400>    14
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln
  1               5                  10                  15 

Thr Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp
             20                  25                  30 

Tyr Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp
         35                  40                  45 

Met Gly Tyr Ile Ser Tyr Ser Gly Ser Ala Arg Tyr Asn Pro Ser Leu
     50                  55                  60 

Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe Ser
 65                  70                  75                  80 

Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Lys Gly Tyr Pro Ala Tyr Phe Ala Tyr Trp Gly Gln Gly Thr
            100                 105                 110 

Leu Val Thr Val Ser Ser
        115            


<210>    15
<211>    107
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Light chain variable region


<400>    15
Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly
  1               5                  10                  15 

Asp Arg Val Ser Ile Ser Cys Lys Ala Ser Gln Asp Val Ser Thr Ala
             20                  25                  30 

Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Glu Leu Leu Ile
         35                  40                  45 

Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly
     50                  55                  60 

Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Val Gln Ala
 65                  70                  75                  80 

Glu Asp Leu Ala Val Tyr Tyr Cys Gln His His Tyr Ser Thr Pro Tyr
                 85                  90                  95 

Thr Phe Gly Gly Gly Thr Lys Leu Glu Met Lys
            100                 105        


<210>    16
<211>    107
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Light chain variable region


<400>    16
Asp Ile Val Met Thr Gln Ser His Ser Phe Leu Ser Ala Ser Val Gly
  1               5                  10                  15 

Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Thr Ala
             20                  25                  30 

Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Glu Leu Leu Ile
         35                  40                  45 

Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly
     50                  55                  60 

Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala
 65                  70                  75                  80 

Glu Asp Val Ala Val Tyr Tyr Cys Gln His His Tyr Ser Thr Pro Tyr
                 85                  90                  95 

Thr Phe Gly Gln Gly Thr Lys Leu Glu Met Lys
            100                 105        


<210>    17
<211>    107
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Light chain variable region


<400>    17
Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
  1               5                  10                  15 

Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Thr Ala
             20                  25                  30 

Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile
         35                  40                  45 

Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly
     50                  55                  60 

Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala
 65                  70                  75                  80 

Glu Asp Val Ala Val Tyr Tyr Cys Gln His His Tyr Ser Thr Pro Tyr
                 85                  90                  95 

Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys
            100                 105        


<210>    18
<211>    107
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Light chain variable region


<400>    18
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
  1               5                  10                  15 

Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Thr Ala
             20                  25                  30 

Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile
         35                  40                  45 

Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Asp Arg Phe Ser Gly
     50                  55                  60 

Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala
 65                  70                  75                  80 

Glu Asp Val Ala Val Tyr Tyr Cys Gln His His Tyr Ser Thr Pro Tyr
                 85                  90                  95 

Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys
            100                 105        


<210>    19
<211>    107
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Light chain variable region


<400>    19
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
  1               5                  10                  15 

Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala
             20                  25                  30 

Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile
         35                  40                  45 

Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Asp Arg Phe Ser Gly
     50                  55                  60 

Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala
 65                  70                  75                  80 

Glu Asp Val Ala Val Tyr Tyr Cys Gln His His Tyr Ser Thr Pro Tyr
                 85                  90                  95 

Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys
            100                 105        


<210>    20
<211>    107
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Light chain variable region


<400>    20
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
  1               5                  10                  15 

Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Thr Ala
             20                  25                  30 

Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile
         35                  40                  45 

Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Asp Arg Phe Ser Gly
     50                  55                  60 

Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
 65                  70                  75                  80 

Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His His Tyr Ser Thr Pro Tyr
                 85                  90                  95 

Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys
            100                 105        


<210>    21
<211>    330
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Heavy chain constant region


<400>    21
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys
  1               5                  10                  15 

Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
             20                  25                  30 

Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
         35                  40                  45 

Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
     50                  55                  60 

Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr
 65                  70                  75                  80 

Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys
                 85                  90                  95 

Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
            100                 105                 110 

Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro
        115                 120                 125 

Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys
    130                 135                 140 

Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
145                 150                 155                 160 

Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
                165                 170                 175 

Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu
            180                 185                 190 

His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn
        195                 200                 205 

Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
    210                 215                 220 

Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu
225                 230                 235                 240 

Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
                245                 250                 255 

Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
            260                 265                 270 

Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
        275                 280                 285 

Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
    290                 295                 300 

Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
305                 310                 315                 320 

Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
                325                 330


<210>    22
<211>    107
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Light chain constant region


<400>    22
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu
  1               5                  10                  15 

Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe
             20                  25                  30 

Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln
         35                  40                  45 

Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser
     50                  55                  60 

Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu
 65                  70                  75                  80 

Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser
                 85                  90                  95 

Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys
            100                 105        


<210>    23
<211>    354
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic_7A6 heavy chain variable region coding DNA


<400>    23
gatgtgcagc ttcaggagtc gggacctggc ctggtgaaac cttctcagtc tctgtccctc         60

acctgcactg tcactggcta ctcaatcacc agtgattatg cctggaactg gatccggcag        120

tttccaggaa acaaactgga gtggatgggc tacataagct acagtggtag cgctcgctac        180

aacccatctc tcaaaagtcg aatctctatc actcgagaca catccatgaa ccagttcttc        240

ctgcagttga attctgtgac tgctgaggac acagccacat attactgtgc aagaaagggg        300

taccctgcct actttgctta ctggggccaa gggactctgg tcactgtctc tgca              354


<210>    24
<211>    321
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic_7A6 light chain variable region coding DNA


<400>    24
gacattgtga tgacccagtc tcacaaattc atgtccacat cagtaggaga cagggtcagc         60

atctcctgca aggccagtca ggatgtgagt actgctgtag cctggtatca acagaaacca        120

ggacaatctc ctgaactact gatttactcg gcatcctacc ggtacactgg agtccctgat        180

cgcttcactg gcagtggatc tgggacggat ttcactttca ccatcagcag tgtgcaggct        240

gaagacctgg cagtttatta ctgtcagcat cattatagta ctccgtacac gttcggaggg        300

gggaccaagc tggaaatgaa a                                                  321


<210>    25
<211>    447
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_7A6 VH3/Vk5-DLE heavy chain


<400>    25
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln
  1               5                  10                  15 

Thr Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp
             20                  25                  30 

Tyr Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp
         35                  40                  45 

Met Gly Tyr Ile Ser Tyr Ser Gly Ser Ala Arg Tyr Asn Pro Ser Leu
     50                  55                  60 

Lys Ser Arg Val Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Phe Ser
 65                  70                  75                  80 

Leu Lys Leu Ser Ser Val Thr Ala Glu Asp Thr Ala Thr Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Lys Gly Tyr Pro Ala Tyr Phe Ala Tyr Trp Gly Gln Gly Thr
            100                 105                 110 

Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
        115                 120                 125 

Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
    130                 135                 140 

Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145                 150                 155                 160 

Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
                165                 170                 175 

Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
            180                 185                 190 

Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
        195                 200                 205 

Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
    210                 215                 220 

His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Asp
225                 230                 235                 240 

Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
                245                 250                 255 

Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
            260                 265                 270 

Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
        275                 280                 285 

Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
    290                 295                 300 

Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305                 310                 315                 320 

Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Leu Pro Glu Glu Lys Thr
                325                 330                 335 

Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
            340                 345                 350 

Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
        355                 360                 365 

Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
    370                 375                 380 

Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385                 390                 395                 400 

Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
                405                 410                 415 

Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
            420                 425                 430 

Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
        435                 440                 445 




<210>    26
<211>    448
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_VH3/Vk5 N298A heavy chain


<400>    26
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln
  1               5                  10                  15 

Thr Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp
             20                  25                  30 

Tyr Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp
         35                  40                  45 

Met Gly Tyr Ile Ser Tyr Ser Gly Ser Ala Arg Tyr Asn Pro Ser Leu
     50                  55                  60 

Lys Ser Arg Val Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Phe Ser
 65                  70                  75                  80 

Leu Lys Leu Ser Ser Val Thr Ala Glu Asp Thr Ala Thr Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Lys Gly Tyr Pro Ala Tyr Phe Ala Tyr Trp Gly Gln Gly Thr
            100                 105                 110 

Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
        115                 120                 125 

Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
    130                 135                 140 

Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145                 150                 155                 160 

Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
                165                 170                 175 

Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
            180                 185                 190 

Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
        195                 200                 205 

Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
    210                 215                 220 

His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser
225                 230                 235                 240 

Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
                245                 250                 255 

Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
            260                 265                 270 

Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
        275                 280                 285 

Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr Arg Val Val
    290                 295                 300 

Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305                 310                 315                 320 

Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
                325                 330                 335 

Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
            340                 345                 350 

Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
        355                 360                 365 

Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
    370                 375                 380 

Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385                 390                 395                 400 

Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
                405                 410                 415 

Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
            420                 425                 430 

Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    27
<211>    448
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_7A6 VH3/Vk5 AAA heavy chain


<400>    27
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln
  1               5                  10                  15 

Thr Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp
             20                  25                  30 

Tyr Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp
         35                  40                  45 

Met Gly Tyr Ile Ser Tyr Ser Gly Ser Ala Arg Tyr Asn Pro Ser Leu
     50                  55                  60 

Lys Ser Arg Val Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Phe Ser
 65                  70                  75                  80 

Leu Lys Leu Ser Ser Val Thr Ala Glu Asp Thr Ala Thr Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Lys Gly Tyr Pro Ala Tyr Phe Ala Tyr Trp Gly Gln Gly Thr
            100                 105                 110 

Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
        115                 120                 125 

Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
    130                 135                 140 

Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145                 150                 155                 160 

Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
                165                 170                 175 

Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
            180                 185                 190 

Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
        195                 200                 205 

Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
    210                 215                 220 

His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser
225                 230                 235                 240 

Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
                245                 250                 255 

Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
            260                 265                 270 

Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
        275                 280                 285 

Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ala Thr Tyr Arg Val Val
    290                 295                 300 

Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305                 310                 315                 320 

Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Ala Ala Thr
                325                 330                 335 

Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
            340                 345                 350 

Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
        355                 360                 365 

Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
    370                 375                 380 

Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385                 390                 395                 400 

Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
                405                 410                 415 

Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
            420                 425                 430 

Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    28
<211>    448
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_7A6 VH3/Vk5 LALAPG heavy chain


<400>    28
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln
  1               5                  10                  15 

Thr Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp
             20                  25                  30 

Tyr Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp
         35                  40                  45 

Met Gly Tyr Ile Ser Tyr Ser Gly Ser Ala Arg Tyr Asn Pro Ser Leu
     50                  55                  60 

Lys Ser Arg Val Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Phe Ser
 65                  70                  75                  80 

Leu Lys Leu Ser Ser Val Thr Ala Glu Asp Thr Ala Thr Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Lys Gly Tyr Pro Ala Tyr Phe Ala Tyr Trp Gly Gln Gly Thr
            100                 105                 110 

Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
        115                 120                 125 

Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
    130                 135                 140 

Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145                 150                 155                 160 

Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
                165                 170                 175 

Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
            180                 185                 190 

Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
        195                 200                 205 

Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
    210                 215                 220 

His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser
225                 230                 235                 240 

Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
                245                 250                 255 

Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
            260                 265                 270 

Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
        275                 280                 285 

Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
    290                 295                 300 

Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305                 310                 315                 320 

Lys Cys Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr
                325                 330                 335 

Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
            340                 345                 350 

Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys
        355                 360                 365 

Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
    370                 375                 380 

Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385                 390                 395                 400 

Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
                405                 410                 415 

Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
            420                 425                 430 

Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    29
<211>    214
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Light chain of 7A6 VH3/Vk5 mutants


<400>    29
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
  1               5                  10                  15 

Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Thr Ala
             20                  25                  30 

Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile
         35                  40                  45 

Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Asp Arg Phe Ser Gly
     50                  55                  60 

Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
 65                  70                  75                  80 

Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His His Tyr Ser Thr Pro Tyr
                 85                  90                  95 

Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala
            100                 105                 110 

Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly
        115                 120                 125 

Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala
    130                 135                 140 

Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln
145                 150                 155                 160 

Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser
                165                 170                 175 

Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr
            180                 185                 190 

Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser
        195                 200                 205 

Phe Asn Arg Gly Glu Cys
    210                


<210>    30
<211>    244
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_human TIGIT


<400>    30
Met Arg Trp Cys Leu Leu Leu Ile Trp Ala Gln Gly Leu Arg Gln Ala
  1               5                  10                  15 

Pro Leu Ala Ser Gly Met Met Thr Gly Thr Ile Glu Thr Thr Gly Asn
             20                  25                  30 

Ile Ser Ala Glu Lys Gly Gly Ser Ile Ile Leu Gln Cys His Leu Ser
         35                  40                  45 

Ser Thr Thr Ala Gln Val Thr Gln Val Asn Trp Glu Gln Gln Asp Gln
     50                  55                  60 

Leu Leu Ala Ile Cys Asn Ala Asp Leu Gly Trp His Ile Ser Pro Ser
 65                  70                  75                  80 

Phe Lys Asp Arg Val Ala Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln
                 85                  90                  95 

Ser Leu Thr Val Asn Asp Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr
            100                 105                 110 

Tyr Pro Asp Gly Thr Tyr Thr Gly Arg Ile Phe Leu Glu Val Leu Glu
        115                 120                 125 

Ser Ser Val Ala Glu His Gly Ala Arg Phe Gln Ile Pro Leu Leu Gly
    130                 135                 140 

Ala Met Ala Ala Thr Leu Val Val Ile Cys Thr Ala Val Ile Val Val
145                 150                 155                 160 

Val Ala Leu Thr Arg Lys Lys Lys Ala Leu Arg Ile His Ser Val Glu
                165                 170                 175 

Gly Asp Leu Arg Arg Lys Ser Ala Gly Gln Glu Glu Trp Ser Pro Ser
            180                 185                 190 

Ala Pro Ser Pro Pro Gly Ser Cys Val Gln Ala Glu Ala Ala Pro Ala
        195                 200                 205 

Gly Leu Cys Gly Glu Gln Arg Gly Glu Asp Cys Ala Glu Leu His Asp
    210                 215                 220 

Tyr Phe Asn Val Leu Ser Tyr Arg Ser Leu Gly Asn Cys Ser Phe Phe
225                 230                 235                 240 

Thr Glu Thr Gly
               


<210>    31
<211>    20
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_Epitope region on human TIGIT


<400>    31
Thr Ala Gln Val Thr Gln Val Asn Trp Glu Gln Gln Asp Gln Leu Leu
  1               5                  10                  15 

Ala Ile Cys Asn
             20
