                         SEQUENCE LISTING

<110>  KONIKU INC.
 
<120>  ENGINEERED CELLS FOR EXPRESSING OLFACTORY RECEPTORS AND METHODS 
       FOR THEIR USE

<130>  141583-8012.WO00

<140>  PCT/US 22/29315
<141>  2022-05-13

<150>  US 63/189,015
<151>  2021-05-14

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  317
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wild type mOR254-2

<400>  1

Met Glu Met Asp Val Tyr Asn Leu Thr Thr Val Thr Gln Phe Ile Leu 
1               5                   10                  15      


Ile Gly Leu Ser Asp Leu Pro Glu Val Arg Tyr Pro Leu Phe Val Ala 
            20                  25                  30          


Phe Val Ile Ile Tyr Gln Ile Thr Leu Leu Gly Asn Gly Leu Ile Leu 
        35                  40                  45              


Leu Ala Ile Val Thr Glu Lys Lys Leu Gln Thr Pro Met Tyr Tyr Leu 
    50                  55                  60                  


Leu Ala Asn Leu Ser Leu Leu Asp Ile Phe Cys Pro Ser Ala Thr Val 
65                  70                  75                  80  


Pro Lys Met Leu Lys Asn Leu Leu Thr Glu Asp His Ser Ile Ser Phe 
                85                  90                  95      


Val Gly Cys Ala Leu Gln Leu Tyr Phe Leu Val Ala Leu Ala Gly Thr 
            100                 105                 110         


Glu Val Phe Leu Leu Ala Val Met Ala Tyr Asp Arg Tyr Val Ala Ile 
        115                 120                 125             


Cys Phe Pro Leu Arg Tyr Ser Leu Ile Met Thr Lys Val Arg Cys Val 
    130                 135                 140                 


Gln Leu Leu Phe Gly Thr Trp Ala Ala Gly Phe Leu Asn Ser Phe Val 
145                 150                 155                 160 


His Thr Met Ser Thr Phe Ser Leu Ser Phe Cys Lys Ser Asn Arg Val 
                165                 170                 175     


Asn Gln Tyr Tyr Cys Asp Ile Pro Pro Val Val Ala Leu Ser Cys Ser 
            180                 185                 190         


Ser Thr Tyr Met Ala Glu Val Leu Leu Leu Val Ile Ala Ser Ile Phe 
        195                 200                 205             


Gly Val Gly Ala Phe Leu Ile Thr Leu Ile Ser Tyr Ile Tyr Ile Val 
    210                 215                 220                 


Ser Thr Ile Leu Lys Ile Arg Ser Ala Glu Gly Lys Arg Lys Ala Phe 
225                 230                 235                 240 


Ser Thr Cys Ala Ser His Leu Leu Val Val Phe Leu Phe Tyr Gly Thr 
                245                 250                 255     


Thr Ile Phe Thr Tyr Met Arg Pro Thr Ser Ser Gln His Ser Pro Gly 
            260                 265                 270         


Arg Asp Arg Leu Ile Ser Met Leu Tyr Gly Val Ile Thr Pro Met Leu 
        275                 280                 285             


Asn Pro Ile Ile Tyr Ser Leu Arg Asn Thr Glu Val Lys Gly Ala Leu 
    290                 295                 300                 


Arg Lys Val Leu His Leu Arg Ile Cys Ser Gln Thr Ala 
305                 310                 315         


<210>  2
<211>  310
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wild type mOR204-1

<400>  2

Met Glu Ala Gln Asn His Thr Thr Val Lys Glu Phe Ile Leu Leu Gly 
1               5                   10                  15      


Leu Thr Glu Asn Ser Thr Leu Arg Val Ile Leu Phe Met Ile Phe Leu 
            20                  25                  30          


Gly Ile Tyr Thr Val Thr Leu Val Gly Asn Phe Ser Ile Ile Ser Leu 
        35                  40                  45              


Ile Arg Ser Cys Pro Gln Leu His Thr Pro Met Tyr Leu Phe Leu Ser 
    50                  55                  60                  


His Leu Ala Leu Val Asp Ile Gly Phe Ser Thr Ser Ile Thr Pro Ile 
65                  70                  75                  80  


Met Leu Thr Gly Phe Leu Gly His Thr Val Thr Leu Ser Val Ala Ala 
                85                  90                  95      


Cys Val Ala Gln Phe Cys Ile Ala Val Thr Phe Gly Thr Val Glu Cys 
            100                 105                 110         


Phe Leu Leu Ala Val Met Ala Tyr Asp Arg Tyr Val Ala Ile Cys Ser 
        115                 120                 125             


Pro Leu Leu Tyr Ser Thr His Met Ser Pro Arg Ile Cys Phe Leu Leu 
    130                 135                 140                 


Val Gly Ala Ser Tyr Val Gly Gly Cys Val Asn Ser Gly Thr Phe Thr 
145                 150                 155                 160 


Ser Cys Leu Leu Ile Leu Ser Phe Cys Gly Pro Asn Gln Ile Asp His 
                165                 170                 175     


Phe Phe Cys Asp Phe Pro Ala Val Leu Lys Leu Ser Cys Ser Asp Val 
            180                 185                 190         


Ser Ile Ile Gly Ile Ile Pro Ser Ile Ser Ala Gly Ser Ile Ile Val 
        195                 200                 205             


Ile Thr Val Phe Val Ile Ala Val Ser Tyr Thr Tyr Ile Leu Ile Thr 
    210                 215                 220                 


Ile Leu Asn Met Arg Ser Thr Glu Gly Arg His Lys Ala Phe Ser Thr 
225                 230                 235                 240 


Cys Thr Ser His Leu Thr Ala Val Thr Leu Tyr Tyr Gly Thr Ile Thr 
                245                 250                 255     


Phe Ile Tyr Val Met Pro Lys Ser Asn Tyr Ser Thr Ala Gln Asn Lys 
            260                 265                 270         


Ile Leu Ser Val Phe Tyr Thr Val Val Ile Pro Met Leu Asn Pro Leu 
        275                 280                 285             


Ile Tyr Ser Leu Arg Asn Arg Asp Val Lys Glu Ala Leu Arg Lys Ala 
    290                 295                 300                 


Ile Ile Arg Ile Phe Pro 
305                 310 


<210>  3
<211>  306
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wild type mOR182-5

<400>  3

Met Glu Val Asn Arg Thr Leu Val Thr Ala Phe Ile Leu Arg Gly Ile 
1               5                   10                  15      


Thr Asp Leu Pro Glu Leu Gln Val Pro Met Phe Leu Val Phe Phe Phe 
            20                  25                  30          


Ile Tyr Val Thr Thr Met Val Gly Asn Leu Gly Leu Ile Val Leu Ile 
        35                  40                  45              


Trp Lys Asp Pro Arg Leu His Thr Pro Met Tyr Phe Phe Leu Gly Ser 
    50                  55                  60                  


Leu Ala Phe Ala Asp Ala Cys Thr Ser Ser Ser Val Thr Pro Arg Met 
65                  70                  75                  80  


Leu Val Asn Ile Leu Asp Asn Gly Lys Met Ile Ser Leu Ser Glu Cys 
                85                  90                  95      


Met Ala Gln Tyr Tyr Val Phe Gly Ser Ser Ala Thr Thr Glu Cys Phe 
            100                 105                 110         


Leu Leu Val Ala Met Ala Tyr Asp Arg Tyr Val Ala Ile Cys Asn Pro 
        115                 120                 125             


Leu Leu Tyr Leu Val Val Met Ser Asn Arg Val Cys Thr Cys Leu Ile 
    130                 135                 140                 


Ser Gly Ser Tyr Ile Ile Gly Phe Leu His Pro Leu Ile His Val Gly 
145                 150                 155                 160 


Leu Leu Phe Arg Leu Thr Phe Cys Lys Ser Asn Ile Ile Asp His Phe 
                165                 170                 175     


Tyr Cys Glu Ile Leu Pro Leu Tyr Thr Ile Ser Cys Thr Asp Pro Ser 
            180                 185                 190         


Ile Asn Ala Phe Val Val Phe Ile Phe Ala Ala Val Ile Gln Ala Val 
        195                 200                 205             


Thr Phe Met Ser Ile Ala Val Ser Tyr Ala His Val Leu Phe Ser Ile 
    210                 215                 220                 


Leu Lys Thr Lys Ser Glu Arg Gly Arg Arg Lys Ala Phe Ser Thr Cys 
225                 230                 235                 240 


Ser Ala His Leu Leu Ser Val Ser Leu Phe Tyr Gly Thr Leu Phe Phe 
                245                 250                 255     


Met Tyr Val Ser Pro Gly Ser Gly Pro Ser Lys Tyr Lys Asn Lys Met 
            260                 265                 270         


Tyr Ser Leu Phe Tyr Thr Ile Val Ile Pro Leu Leu Asn Pro Phe Ile 
        275                 280                 285             


Tyr Ser Leu Arg Asn Lys Glu Val Leu Gly Ala Leu Arg Lys Ile Met 
    290                 295                 300                 


Lys Pro 
305     


<210>  4
<211>  324
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wild type mOR41-1

<400>  4

Met Leu Gly Pro Ser Tyr Asn His Thr Met Glu Ser Pro Gly Thr Phe 
1               5                   10                  15      


Phe Leu Leu Gly Ile Pro Gly Phe Gln Ser Ser Tyr Leu Trp Leu Ala 
            20                  25                  30          


Ile Ser Leu Ser Thr Met Tyr Ser Ile Ala Val Leu Gly Asn Met Leu 
        35                  40                  45              


Ile Ile Ile Val Ile Cys Met Asp Ser Thr Leu Gln Glu Pro Met Tyr 
    50                  55                  60                  


Phe Phe Leu Cys Val Leu Ala Ala Val Asp Ile Val Met Ala Ser Ser 
65                  70                  75                  80  


Val Val Pro Lys Met Val Ser Ile Phe Ser Ser Gly Asp Ser Ser Ile 
                85                  90                  95      


Ser Phe Asn Ala Cys Phe Thr Gln Met Tyr Phe Val His Ala Ala Thr 
            100                 105                 110         


Ala Val Glu Thr Gly Leu Leu Leu Ala Met Ala Phe Asp Arg Tyr Val 
        115                 120                 125             


Ala Ile Cys Lys Pro Leu His Tyr Met Arg Ile Leu Thr Arg His Val 
    130                 135                 140                 


Met Leu Gly Ile Ser Val Thr Ile Thr Val Arg Ala Val Ile Phe Met 
145                 150                 155                 160 


Thr Pro Leu Ser Trp Met Leu Ser His Leu Pro Phe Cys Ala Ser Asn 
                165                 170                 175     


Val Val Pro His Ser Tyr Cys Glu His Met Ala Val Ala Lys Leu Ala 
            180                 185                 190         


Cys Ala Asp Pro Met Pro Ser Ser Leu Tyr Ser Leu Ile Phe Ser Ser 
        195                 200                 205             


Ile Ile Val Gly Ser Asp Val Ala Phe Ile Ser Ala Ser Tyr Ser Leu 
    210                 215                 220                 


Ile Leu Lys Ala Val Phe Gly Leu Ser Ser Arg Asn Ala Gln Trp Lys 
225                 230                 235                 240 


Ala Leu Ser Thr Cys Gly Ser His Val Gly Val Met Ala Leu Tyr Tyr 
                245                 250                 255     


Leu Pro Gly Met Ala Ser Ile Tyr Val Ala Trp Leu Gly Gln Asp Arg 
            260                 265                 270         


Val Pro Leu His Thr Gln Val Leu Leu Ala Asp Leu Tyr Leu Ile Ile 
        275                 280                 285             


Pro Pro Thr Leu Asn Pro Ile Ile Tyr Gly Ile Arg Thr Arg Gln Ile 
    290                 295                 300                 


Arg Glu Arg Ile Trp Ser Leu Leu Thr His Cys Phe Phe Ser Gln Cys 
305                 310                 315                 320 


Thr Gln Gly Ser 
                


<210>  5
<211>  317
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant mOR254-2 A110V


<220>
<221>  MISC_FEATURE
<222>  (110)..(100)
<223>  mutated residue

<400>  5

Met Glu Met Asp Val Tyr Asn Leu Thr Thr Val Thr Gln Phe Ile Leu 
1               5                   10                  15      


Ile Gly Leu Ser Asp Leu Pro Glu Val Arg Tyr Pro Leu Phe Val Ala 
            20                  25                  30          


Phe Val Ile Ile Tyr Gln Ile Thr Leu Leu Gly Asn Gly Leu Ile Leu 
        35                  40                  45              


Leu Ala Ile Val Thr Glu Lys Lys Leu Gln Thr Pro Met Tyr Tyr Leu 
    50                  55                  60                  


Leu Ala Asn Leu Ser Leu Leu Asp Ile Phe Cys Pro Ser Ala Thr Val 
65                  70                  75                  80  


Pro Lys Met Leu Lys Asn Leu Leu Thr Glu Asp His Ser Ile Ser Phe 
                85                  90                  95      


Val Gly Cys Ala Leu Gln Leu Tyr Phe Leu Val Ala Leu Val Gly Thr 
            100                 105                 110         


Glu Val Phe Leu Leu Ala Val Met Ala Tyr Asp Arg Tyr Val Ala Ile 
        115                 120                 125             


Cys Phe Pro Leu Arg Tyr Ser Leu Ile Met Thr Lys Val Arg Cys Val 
    130                 135                 140                 


Gln Leu Leu Phe Gly Thr Trp Ala Ala Gly Phe Leu Asn Ser Phe Val 
145                 150                 155                 160 


His Thr Met Ser Thr Phe Ser Leu Ser Phe Cys Lys Ser Asn Arg Val 
                165                 170                 175     


Asn Gln Tyr Tyr Cys Asp Ile Pro Pro Val Val Ala Leu Ser Cys Ser 
            180                 185                 190         


Ser Thr Tyr Met Ala Glu Val Leu Leu Leu Val Ile Ala Ser Ile Phe 
        195                 200                 205             


Gly Val Gly Ala Phe Leu Ile Thr Leu Ile Ser Tyr Ile Tyr Ile Val 
    210                 215                 220                 


Ser Thr Ile Leu Lys Ile Arg Ser Ala Glu Gly Lys Arg Lys Ala Phe 
225                 230                 235                 240 


Ser Thr Cys Ala Ser His Leu Leu Val Val Phe Leu Phe Tyr Gly Thr 
                245                 250                 255     


Thr Ile Phe Thr Tyr Met Arg Pro Thr Ser Ser Gln His Ser Pro Gly 
            260                 265                 270         


Arg Asp Arg Leu Ile Ser Met Leu Tyr Gly Val Ile Thr Pro Met Leu 
        275                 280                 285             


Asn Pro Ile Ile Tyr Ser Leu Arg Asn Thr Glu Val Lys Gly Ala Leu 
    290                 295                 300                 


Arg Lys Val Leu His Leu Arg Ile Cys Ser Gln Thr Ala 
305                 310                 315         


<210>  6
<211>  306
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant mOR182-5 T109S E110S


<220>
<221>  MISC_FEATURE
<222>  (109)..(110)
<223>  mutated residue

<400>  6

Met Glu Val Asn Arg Thr Leu Val Thr Ala Phe Ile Leu Arg Gly Ile 
1               5                   10                  15      


Thr Asp Leu Pro Glu Leu Gln Val Pro Met Phe Leu Val Phe Phe Phe 
            20                  25                  30          


Ile Tyr Val Thr Thr Met Val Gly Asn Leu Gly Leu Ile Val Leu Ile 
        35                  40                  45              


Trp Lys Asp Pro Arg Leu His Thr Pro Met Tyr Phe Phe Leu Gly Ser 
    50                  55                  60                  


Leu Ala Phe Ala Asp Ala Cys Thr Ser Ser Ser Val Thr Pro Arg Met 
65                  70                  75                  80  


Leu Val Asn Ile Leu Asp Asn Gly Lys Met Ile Ser Leu Ser Glu Cys 
                85                  90                  95      


Met Ala Gln Tyr Tyr Val Phe Gly Ser Ser Ala Thr Ser Ser Cys Phe 
            100                 105                 110         


Leu Leu Val Ala Met Ala Tyr Asp Arg Tyr Val Ala Ile Cys Asn Pro 
        115                 120                 125             


Leu Leu Tyr Leu Val Val Met Ser Asn Arg Val Cys Thr Cys Leu Ile 
    130                 135                 140                 


Ser Gly Ser Tyr Ile Ile Gly Phe Leu His Pro Leu Ile His Val Gly 
145                 150                 155                 160 


Leu Leu Phe Arg Leu Thr Phe Cys Lys Ser Asn Ile Ile Asp His Phe 
                165                 170                 175     


Tyr Cys Glu Ile Leu Pro Leu Tyr Thr Ile Ser Cys Thr Asp Pro Ser 
            180                 185                 190         


Ile Asn Ala Phe Val Val Phe Ile Phe Ala Ala Val Ile Gln Ala Val 
        195                 200                 205             


Thr Phe Met Ser Ile Ala Val Ser Tyr Ala His Val Leu Phe Ser Ile 
    210                 215                 220                 


Leu Lys Thr Lys Ser Glu Arg Gly Arg Arg Lys Ala Phe Ser Thr Cys 
225                 230                 235                 240 


Ser Ala His Leu Leu Ser Val Ser Leu Phe Tyr Gly Thr Leu Phe Phe 
                245                 250                 255     


Met Tyr Val Ser Pro Gly Ser Gly Pro Ser Lys Tyr Lys Asn Lys Met 
            260                 265                 270         


Tyr Ser Leu Phe Tyr Thr Ile Val Ile Pro Leu Leu Asn Pro Phe Ile 
        275                 280                 285             


Tyr Ser Leu Arg Asn Lys Glu Val Leu Gly Ala Leu Arg Lys Ile Met 
    290                 295                 300                 


Lys Pro 
305     


<210>  7
<211>  324
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mOR41-1 V114M


<220>
<221>  MISC_FEATURE
<222>  (114)..(114)
<223>  mutated residue

<400>  7

Met Leu Gly Pro Ser Tyr Asn His Thr Met Glu Ser Pro Gly Thr Phe 
1               5                   10                  15      


Phe Leu Leu Gly Ile Pro Gly Phe Gln Ser Ser Tyr Leu Trp Leu Ala 
            20                  25                  30          


Ile Ser Leu Ser Thr Met Tyr Ser Ile Ala Val Leu Gly Asn Met Leu 
        35                  40                  45              


Ile Ile Ile Val Ile Cys Met Asp Ser Thr Leu Gln Glu Pro Met Tyr 
    50                  55                  60                  


Phe Phe Leu Cys Val Leu Ala Ala Val Asp Ile Val Met Ala Ser Ser 
65                  70                  75                  80  


Val Val Pro Lys Met Val Ser Ile Phe Ser Ser Gly Asp Ser Ser Ile 
                85                  90                  95      


Ser Phe Asn Ala Cys Phe Thr Gln Met Tyr Phe Val His Ala Ala Thr 
            100                 105                 110         


Ala Ala Glu Thr Gly Leu Leu Leu Ala Met Ala Phe Asp Arg Tyr Val 
        115                 120                 125             


Ala Ile Cys Lys Pro Leu His Tyr Met Arg Ile Leu Thr Arg His Val 
    130                 135                 140                 


Met Leu Gly Ile Ser Val Thr Ile Thr Val Arg Ala Val Ile Phe Met 
145                 150                 155                 160 


Thr Pro Leu Ser Trp Met Leu Ser His Leu Pro Phe Cys Ala Ser Asn 
                165                 170                 175     


Val Val Pro His Ser Tyr Cys Glu His Met Ala Val Ala Lys Leu Ala 
            180                 185                 190         


Cys Ala Asp Pro Met Pro Ser Ser Leu Tyr Ser Leu Ile Phe Ser Ser 
        195                 200                 205             


Ile Ile Val Gly Ser Asp Val Ala Phe Ile Ser Ala Ser Tyr Ser Leu 
    210                 215                 220                 


Ile Leu Lys Ala Val Phe Gly Leu Ser Ser Arg Asn Ala Gln Trp Lys 
225                 230                 235                 240 


Ala Leu Ser Thr Cys Gly Ser His Val Gly Val Met Ala Leu Tyr Tyr 
                245                 250                 255     


Leu Pro Gly Met Ala Ser Ile Tyr Val Ala Trp Leu Gly Gln Asp Arg 
            260                 265                 270         


Val Pro Leu His Thr Gln Val Leu Leu Ala Asp Leu Tyr Leu Ile Ile 
        275                 280                 285             


Pro Pro Thr Leu Asn Pro Ile Ile Tyr Gly Ile Arg Thr Arg Gln Ile 
    290                 295                 300                 


Arg Glu Arg Ile Trp Ser Leu Leu Thr His Cys Phe Phe Ser Gln Cys 
305                 310                 315                 320 


Thr Gln Gly Ser 
                


