               SEQUENCE LISTING



<110> DSM IP ASSETS B.V.

<120> ENHANCING FORMATION OF HMOS BY MODIFYING LACTOSE IMPORT IN THE CELL

<130> 340340-WO-PCT

<150> DKPA202170249

<151> 2021-05-17

<160> 103

<170> BiSSAP 1.3.6

<210> 1

<211> 417

<212> PRT

<213> Escherichia coli





<220> 

<223> Lactose permease LacY GenBank ID: NP_414877.1



<400> 1

Met Tyr Tyr Leu Lys Asn Thr Asn Phe Trp Met Phe Gly Leu Phe Phe 

1               5                   10                  15      

Phe Phe Tyr Phe Phe Ile Met Gly Ala Tyr Phe Pro Phe Phe Pro Ile 

            20                  25                  30          

Trp Leu His Asp Ile Asn His Ile Ser Lys Ser Asp Thr Gly Ile Ile 

        35                  40                  45              

Phe Ala Ala Ile Ser Leu Phe Ser Leu Leu Phe Gln Pro Leu Phe Gly 

    50                  55                  60                  

Leu Leu Ser Asp Lys Leu Gly Leu Arg Lys Tyr Leu Leu Trp Ile Ile 

65                  70                  75                  80  

Thr Gly Met Leu Val Met Phe Ala Pro Phe Phe Ile Phe Ile Phe Gly 

                85                  90                  95      

Pro Leu Leu Gln Tyr Asn Ile Leu Val Gly Ser Ile Val Gly Gly Ile 

            100                 105                 110         

Tyr Leu Gly Phe Cys Phe Asn Ala Gly Ala Pro Ala Val Glu Ala Phe 

        115                 120                 125             

Ile Glu Lys Val Ser Arg Arg Ser Asn Phe Glu Phe Gly Arg Ala Arg 

    130                 135                 140                 

Met Phe Gly Cys Val Gly Trp Ala Leu Cys Ala Ser Ile Val Gly Ile 

145                 150                 155                 160 

Met Phe Thr Ile Asn Asn Gln Phe Val Phe Trp Leu Gly Ser Gly Cys 

                165                 170                 175     

Ala Leu Ile Leu Ala Val Leu Leu Phe Phe Ala Lys Thr Asp Ala Pro 

            180                 185                 190         

Ser Ser Ala Thr Val Ala Asn Ala Val Gly Ala Asn His Ser Ala Phe 

        195                 200                 205             

Ser Leu Lys Leu Ala Leu Glu Leu Phe Arg Gln Pro Lys Leu Trp Phe 

    210                 215                 220                 

Leu Ser Leu Tyr Val Ile Gly Val Ser Cys Thr Tyr Asp Val Phe Asp 

225                 230                 235                 240 

Gln Gln Phe Ala Asn Phe Phe Thr Ser Phe Phe Ala Thr Gly Glu Gln 

                245                 250                 255     

Gly Thr Arg Val Phe Gly Tyr Val Thr Thr Met Gly Glu Leu Leu Asn 

            260                 265                 270         

Ala Ser Ile Met Phe Phe Ala Pro Leu Ile Ile Asn Arg Ile Gly Gly 

        275                 280                 285             

Lys Asn Ala Leu Leu Leu Ala Gly Thr Ile Met Ser Val Arg Ile Ile 

    290                 295                 300                 

Gly Ser Ser Phe Ala Thr Ser Ala Leu Glu Val Val Ile Leu Lys Thr 

305                 310                 315                 320 

Leu His Met Phe Glu Val Pro Phe Leu Leu Val Gly Cys Phe Lys Tyr 

                325                 330                 335     

Ile Thr Ser Gln Phe Glu Val Arg Phe Ser Ala Thr Ile Tyr Leu Val 

            340                 345                 350         

Cys Phe Cys Phe Phe Lys Gln Leu Ala Met Ile Phe Met Ser Val Leu 

        355                 360                 365             

Ala Gly Asn Met Tyr Glu Ser Ile Gly Phe Gln Gly Ala Tyr Leu Val 

    370                 375                 380                 

Leu Gly Leu Val Ala Leu Gly Phe Thr Leu Ile Ser Val Phe Thr Leu 

385                 390                 395                 400 

Ser Gly Pro Gly Pro Leu Ser Leu Leu Arg Arg Gln Val Asn Glu Val 

                405                 410                 415     

Ala 

    



<210> 2

<211> 1254

<212> DNA

<213> Escherichia coli K-12





<220> 

<223> Nucleic acid sequence encoding LacY, GenBank ID: NC_000913.3



<400> 2

atgtactatt taaaaaacac aaacttttgg atgttcggtt tattcttttt cttttacttt      60



tttatcatgg gagcctactt cccgtttttc ccgatttggc tacatgacat caaccatatc     120



agcaaaagtg atacgggtat tatttttgcc gctatttctc tgttctcgct attattccaa     180



ccgctgtttg gtctgctttc tgacaaactc gggctgcgca aatacctgct gtggattatt     240



accggcatgt tagtgatgtt tgcgccgttc tttattttta tcttcgggcc actgttacaa     300



tacaacattt tagtaggatc gattgttggt ggtatttatc taggcttttg ttttaacgcc     360



ggtgcgccag cagtagaggc atttattgag aaagtcagcc gtcgcagtaa tttcgaattt     420



ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc     480



atgttcacca tcaataatca gtttgttttc tggctgggct ctggctgtgc actcatcctc     540



gccgttttac tctttttcgc caaaacggat gcgccctctt ctgccacggt tgccaatgcg     600



gtaggtgcca accattcggc atttagcctt aagctggcac tggaactgtt cagacagcca     660



aaactgtggt ttttgtcact gtatgttatt ggcgtttcct gcacctacga tgtttttgac     720



caacagtttg ctaatttctt tacttcgttc tttgctaccg gtgaacaggg tacgcgggta     780



tttggctacg taacgacaat gggcgaatta cttaacgcct cgattatgtt ctttgcgcca     840



ctgatcatta atcgcatcgg tgggaaaaac gccctgctgc tggctggcac tattatgtct     900



gtacgtatta ttggctcatc gttcgccacc tcagcgctgg aagtggttat tctgaaaacg     960



ctgcatatgt ttgaagtacc gttcctgctg gtgggctgct ttaaatatat taccagccag    1020



tttgaagtgc gtttttcagc gacgatttat ctggtctgtt tctgcttctt taagcaactg    1080



gcgatgattt ttatgtctgt actggcgggc aatatgtatg aaagcatcgg tttccagggc    1140



gcttatctgg tgctgggtct ggtggcgctg ggcttcacct taatttccgt gttcacgctt    1200



agcggccccg gcccgctttc cctgctgcgt cgtcaggtga atgaagtcgc ttaa          1254





<210> 3

<211> 433

<212> PRT

<213> Escherichia coli





<220> 

<223> Lactose permease, GenBank ID: WP_042094275.1



<400> 3

Met Tyr Tyr Leu Lys Asn Thr Asn Phe Trp Met Phe Gly Leu Phe Phe 

1               5                   10                  15      

Phe Phe Tyr Phe Phe Ile Met Gly Ala Tyr Phe Pro Phe Phe Pro Ile 

            20                  25                  30          

Trp Leu His Asp Ile Asn His Ile Ser Lys Ser Asp Thr Gly Ile Ile 

        35                  40                  45              

Phe Ala Ala Ile Ser Leu Phe Ser Leu Leu Phe Gln Pro Leu Phe Gly 

    50                  55                  60                  

Leu Leu Ser Asp Lys Leu Gly Leu Arg Lys Tyr Leu Leu Trp Ile Ile 

65                  70                  75                  80  

Thr Gly Met Leu Val Met Phe Ala Pro Phe Phe Ile Phe Ile Phe Gly 

                85                  90                  95      

Pro Leu Leu Gln Tyr Asn Ile Leu Val Gly Ser Ile Val Gly Gly Ile 

            100                 105                 110         

Tyr Leu Gly Phe Cys Phe Asn Ala Gly Ala Pro Ala Val Glu Ala Phe 

        115                 120                 125             

Ile Glu Lys Val Ser Arg Arg Ser Asn Phe Glu Phe Gly Arg Ala Arg 

    130                 135                 140                 

Met Phe Gly Cys Val Gly Trp Ala Leu Cys Ala Ser Ile Val Gly Ile 

145                 150                 155                 160 

Met Phe Thr Ile Asn Asn Gln Phe Val Phe Trp Leu Gly Ser Gly Cys 

                165                 170                 175     

Ala Leu Ile Leu Ala Val Leu Leu Phe Phe Ala Lys Thr Asp Ala Pro 

            180                 185                 190         

Ser Ser Ala Thr Val Ala Asn Ala Val Gly Ala Asn His Ser Ala Phe 

        195                 200                 205             

Ser Leu Lys Leu Ala Leu Glu Leu Phe Arg Gln Pro Lys Leu Trp Phe 

    210                 215                 220                 

Leu Ser Leu Tyr Val Ile Gly Val Ser Cys Thr Tyr Asp Val Phe Asp 

225                 230                 235                 240 

Gln Gln Phe Ala Asn Phe Phe Thr Ser Phe Phe Ala Thr Gly Glu Gln 

                245                 250                 255     

Gly Thr Arg Val Phe Gly Tyr Val Thr Thr Met Gly Glu Leu Leu Asn 

            260                 265                 270         

Ala Ser Ile Met Phe Phe Ala Pro Leu Ile Ile Asn Arg Ile Gly Gly 

        275                 280                 285             

Lys Asn Ala Leu Leu Leu Ala Gly Thr Ile Met Ser Val Arg Ile Ile 

    290                 295                 300                 

Gly Ser Ser Phe Ala Thr Ser Ala Leu Glu Val Val Ile Leu Lys Thr 

305                 310                 315                 320 

Leu His Met Phe Glu Val Pro Phe Leu Leu Val Gly Cys Phe Lys Tyr 

                325                 330                 335     

Ile Thr Ser Gln Phe Glu Val Arg Phe Ser Ala Thr Ile Tyr Leu Val 

            340                 345                 350         

Cys Phe Cys Phe Phe Lys Gln Leu Ala Met Ile Phe Met Ser Val Leu 

        355                 360                 365             

Ala Gly Asn Met Tyr Glu Ser Ile Gly Phe Gln Gly Ala Tyr Leu Val 

    370                 375                 380                 

Leu Gly Leu Val Ala Leu Gly Phe Thr Leu Ile Ser Val Phe Thr Leu 

385                 390                 395                 400 

Ser Gly Pro Gly Pro Leu Ser Leu Leu Arg Arg Gln Val Asn Glu Val 

                405                 410                 415     

Ala Tyr Gly Cys Gly Ala Ser Ala Leu Ser Asp Gln His Ile Arg Ala 

            420                 425                 430         

Glu 

    



<210> 4

<211> 417

<212> PRT

<213> Proteobacteria





<220> 

<223> Lactose permease, GenBank ID: WP_000291549.1



<400> 4

Met Tyr Tyr Leu Lys Asn Thr Asn Phe Trp Met Phe Gly Leu Phe Phe 

1               5                   10                  15      

Phe Phe Tyr Phe Phe Ile Met Gly Ala Tyr Phe Pro Phe Phe Pro Ile 

            20                  25                  30          

Trp Leu His Asp Ile Asn His Ile Ser Lys Ser Asp Thr Gly Ile Ile 

        35                  40                  45              

Phe Ala Ala Ile Ser Leu Phe Ser Leu Leu Phe Gln Pro Leu Phe Gly 

    50                  55                  60                  

Leu Leu Ser Asp Lys Leu Gly Leu Arg Lys Tyr Leu Leu Trp Ile Ile 

65                  70                  75                  80  

Thr Gly Met Leu Val Met Phe Ala Pro Phe Phe Ile Phe Ile Phe Gly 

                85                  90                  95      

Pro Leu Leu Gln Tyr Asn Ile Leu Val Gly Ser Ile Val Gly Gly Ile 

            100                 105                 110         

Tyr Leu Gly Phe Cys Phe Asn Ala Gly Ala Pro Ala Val Glu Ala Phe 

        115                 120                 125             

Ile Glu Lys Val Ser Arg Arg Ser Asn Phe Glu Phe Gly Arg Ala Arg 

    130                 135                 140                 

Met Phe Gly Cys Val Gly Trp Ala Leu Cys Ala Ser Ile Val Gly Ile 

145                 150                 155                 160 

Met Phe Thr Ile Asn Asn Gln Phe Val Phe Trp Leu Gly Ser Gly Cys 

                165                 170                 175     

Ala Leu Ile Leu Ala Val Leu Leu Phe Phe Ala Lys Thr Asp Ala Pro 

            180                 185                 190         

Ser Ser Ala Thr Val Ala Asn Ala Val Gly Ala Asn His Ser Ala Phe 

        195                 200                 205             

Ser Leu Lys Leu Ala Leu Glu Leu Phe Arg Gln Pro Lys Leu Trp Phe 

    210                 215                 220                 

Leu Ser Leu Tyr Val Ile Gly Val Ser Cys Thr Tyr Asp Val Phe Asp 

225                 230                 235                 240 

Gln Gln Phe Ala Asn Phe Phe Thr Ser Phe Phe Ala Thr Gly Glu Gln 

                245                 250                 255     

Gly Thr Arg Val Phe Gly Tyr Val Thr Thr Met Gly Glu Leu Leu Asn 

            260                 265                 270         

Ala Ser Ile Met Phe Phe Ala Pro Leu Ile Ile Asn Arg Ile Gly Gly 

        275                 280                 285             

Lys Asn Ala Leu Leu Leu Ala Gly Thr Ile Met Ser Val Arg Ile Ile 

    290                 295                 300                 

Gly Ser Ser Phe Ala Thr Ser Ala Leu Glu Val Val Ile Leu Lys Thr 

305                 310                 315                 320 

Leu His Met Phe Glu Val Pro Phe Leu Leu Val Gly Cys Phe Lys Tyr 

                325                 330                 335     

Ile Thr Ser Gln Phe Glu Val Arg Phe Ser Ala Thr Ile Tyr Leu Val 

            340                 345                 350         

Cys Phe Cys Phe Phe Lys Gln Leu Ala Met Ile Phe Met Ser Val Leu 

        355                 360                 365             

Ala Gly Asn Met Tyr Glu Ser Ile Gly Phe Gln Gly Ala Tyr Leu Val 

    370                 375                 380                 

Leu Gly Leu Val Ala Leu Gly Phe Thr Leu Ile Ser Val Phe Thr Leu 

385                 390                 395                 400 

Ser Gly Pro Gly Pro Leu Ser Leu Leu Arg Arg Gln Val Asn Glu Val 

                405                 410                 415     

Ala 

    



<210> 5

<211> 417

<212> PRT

<213> Campylobacter jejuni





<220> 

<223> Lactose permease GenBank ID: WP_089607162.1



<400> 5

Met Tyr Tyr Leu Lys Asn Thr Asn Phe Trp Met Phe Gly Leu Phe Phe 

1               5                   10                  15      

Phe Phe Tyr Phe Phe Ile Met Gly Ala Tyr Phe Pro Phe Phe Pro Ile 

            20                  25                  30          

Trp Leu His Asp Ile Asn His Ile Ser Lys Ser Asp Thr Gly Ile Ile 

        35                  40                  45              

Phe Ala Ala Ile Ser Leu Phe Ser Leu Leu Phe Gln Pro Leu Phe Gly 

    50                  55                  60                  

Leu Leu Ser Asp Lys Leu Gly Leu Arg Lys Tyr Leu Leu Trp Ile Ile 

65                  70                  75                  80  

Thr Gly Met Leu Val Met Phe Ala Pro Phe Phe Ile Phe Ile Phe Gly 

                85                  90                  95      

Pro Leu Leu Gln Tyr Asn Ile Leu Val Gly Ser Ile Val Gly Gly Ile 

            100                 105                 110         

Tyr Leu Gly Phe Cys Phe Asn Ala Gly Ala Pro Ala Val Glu Ala Phe 

        115                 120                 125             

Ile Glu Lys Val Ser Arg Arg Ser Asn Phe Glu Phe Gly Arg Ala Arg 

    130                 135                 140                 

Met Phe Gly Cys Val Gly Trp Ala Leu Cys Ala Ser Ile Val Gly Ile 

145                 150                 155                 160 

Met Phe Thr Ile Asn Asn Gln Phe Val Phe Trp Leu Gly Ser Gly Cys 

                165                 170                 175     

Cys Leu Ile Leu Ala Val Leu Leu Phe Phe Ala Lys Thr Asp Ala Pro 

            180                 185                 190         

Ser Ser Ala Thr Val Ala Asn Ala Val Gly Ala Asn His Ser Ala Phe 

        195                 200                 205             

Ser Leu Lys Leu Ala Leu Glu Leu Phe Arg Gln Pro Lys Leu Trp Phe 

    210                 215                 220                 

Leu Ser Leu Tyr Val Ile Gly Val Ser Cys Thr Tyr Asp Val Phe Asp 

225                 230                 235                 240 

Gln Gln Phe Ala Asn Phe Phe Thr Ser Phe Phe Ala Thr Gly Glu Gln 

                245                 250                 255     

Gly Thr Arg Val Phe Gly Tyr Val Thr Thr Met Gly Glu Leu Leu Asn 

            260                 265                 270         

Ala Ser Ile Met Phe Phe Ala Pro Leu Ile Ile Asn Arg Ile Gly Gly 

        275                 280                 285             

Lys Asn Ala Leu Leu Leu Ala Gly Thr Ile Met Ser Val Arg Ile Ile 

    290                 295                 300                 

Gly Ser Ser Phe Ala Thr Ser Ala Leu Glu Val Val Ile Leu Lys Thr 

305                 310                 315                 320 

Leu His Met Phe Glu Val Pro Phe Leu Leu Val Gly Cys Phe Lys Tyr 

                325                 330                 335     

Ile Thr Ser Gln Phe Glu Val Arg Phe Ser Ala Thr Ile Tyr Leu Val 

            340                 345                 350         

Cys Phe Cys Phe Phe Lys Gln Leu Ala Met Ile Phe Met Ser Val Leu 

        355                 360                 365             

Ala Gly Asn Met Tyr Glu Ser Ile Gly Phe Gln Gly Ala Tyr Leu Val 

    370                 375                 380                 

Leu Gly Leu Val Ala Leu Gly Phe Thr Leu Ile Ser Val Phe Thr Leu 

385                 390                 395                 400 

Ser Gly Pro Gly Pro Leu Ser Leu Leu Arg Arg Gln Val Asn Glu Val 

                405                 410                 415     

Ala 

    



<210> 6

<211> 417

<212> PRT

<213> Klebsiella pneumoniae





<220> 

<223> Lactose permease, GenBank ID: WP_152280604.1



<400> 6

Met Tyr Tyr Leu Lys Asn Thr Asn Phe Trp Met Phe Gly Leu Phe Phe 

1               5                   10                  15      

Phe Phe Tyr Phe Phe Ile Met Gly Ala Tyr Phe Pro Phe Phe Pro Ile 

            20                  25                  30          

Trp Leu His Asp Ile Asn His Ile Ser Lys Ser Asp Thr Gly Ile Ile 

        35                  40                  45              

Phe Ala Ala Ile Ser Leu Phe Ser Leu Leu Phe Gln Pro Leu Phe Gly 

    50                  55                  60                  

Leu Leu Ser Asp Lys Leu Gly Leu Arg Lys Tyr Leu Leu Trp Ile Ile 

65                  70                  75                  80  

Thr Gly Met Leu Val Met Phe Ala Pro Phe Phe Ile Phe Ile Phe Gly 

                85                  90                  95      

Pro Leu Leu Gln Tyr Asn Ile Leu Val Gly Ser Ile Val Gly Gly Ile 

            100                 105                 110         

Tyr Leu Gly Phe Cys Phe Asn Ala Gly Ala Pro Ala Val Glu Ala Phe 

        115                 120                 125             

Ile Glu Lys Val Ser Arg Arg Ser Asn Phe Glu Phe Gly Arg Ala Arg 

    130                 135                 140                 

Met Phe Gly Cys Val Gly Trp Ala Leu Cys Ala Ser Ile Val Gly Ile 

145                 150                 155                 160 

Met Phe Thr Ile Asn Asn Gln Phe Val Phe Trp Leu Gly Ser Gly Cys 

                165                 170                 175     

Ala Leu Ile Leu Ala Val Leu Leu Phe Phe Ala Lys Thr Asp Ala Pro 

            180                 185                 190         

Ser Ser Ala Thr Val Ala Asn Ala Val Gly Ala Asn His Ser Ala Phe 

        195                 200                 205             

Ser Leu Lys Leu Ala Leu Glu Leu Phe Arg Gln Pro Lys Leu Trp Phe 

    210                 215                 220                 

Leu Ser Leu Tyr Val Ile Gly Val Ser Cys Thr Tyr Asp Val Phe Asp 

225                 230                 235                 240 

Gln Gln Phe Ala Asn Phe Phe Thr Ser Phe Phe Ala Thr Gly Glu Gln 

                245                 250                 255     

Gly Thr Arg Val Phe Gly Tyr Val Thr Thr Met Gly Glu Leu Leu Asn 

            260                 265                 270         

Ala Ser Ile Met Phe Phe Ala Pro Leu Ile Ile Asn Arg Ile Gly Gly 

        275                 280                 285             

Lys Asn Ala Leu Leu Leu Ala Gly Thr Ile Met Ser Val Arg Ile Ile 

    290                 295                 300                 

Gly Ser Ser Phe Ala Thr Ser Ala Leu Glu Val Val Ile Leu Lys Thr 

305                 310                 315                 320 

Leu His Met Phe Glu Val Pro Phe Leu Leu Val Gly Cys Phe Lys Tyr 

                325                 330                 335     

Ile Thr Ser Gln Phe Glu Val Arg Phe Ser Ala Thr Ile Tyr Leu Val 

            340                 345                 350         

Cys Phe Cys Phe Phe Lys Gln Leu Ala Met Ile Phe Met Ser Ile Leu 

        355                 360                 365             

Ala Gly Asn Met Tyr Glu Ser Ile Gly Phe Gln Gly Ala Tyr Leu Val 

    370                 375                 380                 

Leu Gly Leu Val Ala Leu Gly Phe Thr Leu Ile Ser Val Phe Thr Leu 

385                 390                 395                 400 

Ser Gly Pro Gly Pro Leu Ser Leu Leu Arg Arg Lys Val Asn Glu Val 

                405                 410                 415     

Ala 

    



<210> 7

<211> 417

<212> PRT

<213> Cronobacter sakazakii





<220> 

<223> Lactose permease, GenBank ID: EGT4952364.1



<400> 7

Met Tyr Tyr Leu Lys Asn Thr Asn Phe Trp Met Phe Gly Leu Phe Phe 

1               5                   10                  15      

Phe Phe Tyr Phe Phe Ile Met Gly Ala Tyr Phe Pro Phe Phe Pro Ile 

            20                  25                  30          

Trp Leu His Asp Ile Asn His Ile Ser Lys Ser Asp Thr Gly Ile Ile 

        35                  40                  45              

Phe Ala Ala Ile Ser Leu Phe Ser Leu Leu Phe Gln Pro Leu Phe Gly 

    50                  55                  60                  

Leu Leu Ser Asp Lys Leu Gly Leu Arg Lys Tyr Leu Leu Trp Ile Ile 

65                  70                  75                  80  

Thr Gly Met Leu Val Met Phe Ala Pro Phe Phe Ile Phe Ile Phe Gly 

                85                  90                  95      

Pro Leu Leu Gln Tyr Asn Ile Leu Val Gly Ser Ile Val Gly Gly Ile 

            100                 105                 110         

Tyr Leu Gly Phe Cys Phe Asn Ala Gly Ala Pro Ala Val Glu Ala Phe 

        115                 120                 125             

Ile Glu Lys Val Ser Arg Arg Ser Asn Phe Glu Tyr Gly Arg Ala Arg 

    130                 135                 140                 

Met Phe Gly Cys Val Gly Trp Ala Leu Cys Ala Ser Ile Val Gly Ile 

145                 150                 155                 160 

Met Phe Thr Ile Asn Asn Gln Phe Val Phe Trp Leu Gly Ser Gly Cys 

                165                 170                 175     

Ala Phe Ile Leu Ala Val Leu Leu Phe Phe Ala Lys Thr Asp Ala Pro 

            180                 185                 190         

Ser Ser Ala Thr Val Ala Asn Ala Val Gly Ala Asn His Ser Ala Phe 

        195                 200                 205             

Ser Leu Lys Leu Ala Leu Glu Leu Phe Arg Gln Pro Lys Leu Trp Phe 

    210                 215                 220                 

Leu Ser Leu Tyr Val Ile Gly Val Ser Cys Thr Tyr Asp Val Phe Asp 

225                 230                 235                 240 

Gln Gln Phe Ala Asn Phe Phe Thr Ser Phe Phe Ala Thr Gly Glu Gln 

                245                 250                 255     

Gly Thr Arg Val Phe Gly Tyr Val Thr Thr Met Gly Glu Leu Leu Asn 

            260                 265                 270         

Ala Ser Ile Met Phe Phe Ala Pro Leu Ile Ile Asn Arg Ile Gly Gly 

        275                 280                 285             

Lys Asn Ala Leu Leu Leu Ala Gly Thr Ile Met Ser Val Arg Ile Ile 

    290                 295                 300                 

Gly Ser Ser Phe Ala Thr Ser Ala Leu Glu Val Val Ile Leu Lys Thr 

305                 310                 315                 320 

Leu His Met Leu Glu Val Pro Phe Leu Leu Val Gly Cys Phe Lys Tyr 

                325                 330                 335     

Ile Thr Ser Gln Phe Glu Val Arg Phe Ser Ala Thr Ile Tyr Leu Val 

            340                 345                 350         

Cys Phe Cys Phe Phe Lys Gln Leu Ala Met Ile Phe Met Ser Ile Leu 

        355                 360                 365             

Ala Gly Asn Met Tyr Glu Ser Ile Gly Phe Gln Gly Ala Tyr Leu Val 

    370                 375                 380                 

Leu Gly Leu Val Ala Leu Gly Phe Thr Leu Ile Ser Val Phe Thr Leu 

385                 390                 395                 400 

Ser Gly Pro Gly Pro Leu Ser Leu Leu Arg Arg Gln Val Asn Glu Val 

                405                 410                 415     

Ala 

    



<210> 8

<211> 417

<212> PRT

<213> Citrobacter freundii





<220> 

<223> Lactose permease, GenBank ID NO: WP_134216118.1



<400> 8

Met Tyr Tyr Leu Lys Asn Thr Asn Phe Trp Met Phe Gly Phe Phe Phe 

1               5                   10                  15      

Phe Phe Tyr Phe Phe Ile Met Gly Ala Tyr Phe Pro Phe Phe Pro Ile 

            20                  25                  30          

Trp Leu His Glu Val Asn His Ile Ser Lys Gly Asp Thr Gly Ile Ile 

        35                  40                  45              

Phe Ala Cys Ile Ser Leu Phe Ser Leu Leu Phe Gln Pro Ile Phe Gly 

    50                  55                  60                  

Leu Leu Ser Asp Lys Leu Gly Leu Arg Lys His Leu Leu Trp Val Ile 

65                  70                  75                  80  

Thr Gly Met Leu Val Met Phe Ala Pro Phe Phe Ile Tyr Val Phe Gly 

                85                  90                  95      

Pro Leu Leu Gln Val Asn Ile Leu Leu Gly Ser Ile Val Gly Gly Ile 

            100                 105                 110         

Tyr Leu Gly Phe Ile Tyr Asn Ala Gly Ala Pro Ala Ile Glu Ala Tyr 

        115                 120                 125             

Ile Glu Lys Ala Ser Arg Arg Ser Asn Phe Glu Phe Gly Arg Ala Arg 

    130                 135                 140                 

Met Phe Gly Cys Val Gly Trp Ala Leu Cys Ala Ser Ile Ala Gly Ile 

145                 150                 155                 160 

Met Phe Thr Ile Asn Asn Gln Phe Val Phe Trp Leu Gly Ser Gly Cys 

                165                 170                 175     

Ala Val Ile Leu Ala Leu Leu Leu Leu Phe Ser Lys Thr Asp Ala Pro 

            180                 185                 190         

Ser Ser Ala Lys Val Ala Asp Ala Val Gly Ala Asn Asn Ser Ala Phe 

        195                 200                 205             

Ser Leu Lys Leu Ala Leu Glu Leu Phe Lys Gln Pro Lys Leu Trp Phe 

    210                 215                 220                 

Leu Ser Leu Tyr Val Val Gly Val Ser Cys Thr Tyr Asp Val Phe Asp 

225                 230                 235                 240 

Gln Gln Phe Ala Asn Phe Phe Thr Ser Leu Phe Ala Thr Gly Glu Gln 

                245                 250                 255     

Gly Thr Arg Val Phe Gly Tyr Val Thr Thr Met Gly Glu Leu Leu Asn 

            260                 265                 270         

Ala Ser Ile Met Phe Phe Ala Pro Leu Ile Val Asn Arg Ile Gly Gly 

        275                 280                 285             

Lys Asn Ala Leu Leu Leu Ala Gly Thr Ile Met Ser Val Arg Ile Ile 

    290                 295                 300                 

Gly Ser Ser Phe Ala Thr Thr Ala Leu Glu Val Val Ile Leu Lys Thr 

305                 310                 315                 320 

Leu His Met Phe Glu Ile Pro Phe Leu Ile Val Gly Cys Phe Lys Tyr 

                325                 330                 335     

Ile Thr Ser Gln Phe Glu Val Arg Phe Ser Ala Thr Ile Tyr Leu Val 

            340                 345                 350         

Cys Phe Cys Phe Phe Lys Gln Leu Ala Met Ile Phe Met Ser Val Leu 

        355                 360                 365             

Ala Gly Lys Met Tyr Glu Ser Ile Gly Phe Gln Gly Ala Tyr Leu Val 

    370                 375                 380                 

Leu Gly Ile Ile Ala Leu Ser Phe Thr Leu Ile Ser Val Phe Thr Leu 

385                 390                 395                 400 

Ser Gly Pro Gly Pro Phe Ser Leu Leu Arg Arg Arg Glu Ser Val Ala 

                405                 410                 415     

Leu 

    



<210> 9

<211> 418

<212> PRT

<213> Citrobacter freundii





<220> 

<223> Lactose permease, GenBank ID: EDI1749185.1



<400> 9

Met Tyr Tyr Leu Lys Asn Thr Asn Phe Trp Met Leu Gly Phe Phe Phe 

1               5                   10                  15      

Phe Phe Tyr Phe Phe Ile Met Gly Ala Tyr Phe Pro Phe Phe Pro Ile 

            20                  25                  30          

Trp Leu His Asp Ile Asn His Ile Ser Lys Gly Asp Thr Gly Ile Ile 

        35                  40                  45              

Phe Ala Cys Ile Ser Leu Phe Ser Leu Leu Phe Gln Pro Val Phe Gly 

    50                  55                  60                  

Leu Met Ser Asp Lys Leu Gly Leu Arg Lys Asn Leu Leu Trp Ile Ile 

65                  70                  75                  80  

Thr Gly Met Leu Val Met Phe Ala Pro Phe Phe Ile Tyr Val Phe Gly 

                85                  90                  95      

Pro Leu Leu His Phe Asn Ile Leu Leu Gly Ser Ile Val Gly Gly Ile 

            100                 105                 110         

Tyr Leu Gly Phe Ile Tyr Asn Ala Gly Ala Pro Ala Ile Glu Ala Tyr 

        115                 120                 125             

Ile Glu Lys Ala Ser Arg Arg Ser His Phe Glu Phe Gly Arg Ala Arg 

    130                 135                 140                 

Met Phe Gly Cys Val Gly Trp Ala Leu Cys Ala Ser Ile Val Gly Val 

145                 150                 155                 160 

Met Phe Thr Ile Asn Asn Glu Phe Val Phe Trp Leu Gly Ser Gly Cys 

                165                 170                 175     

Ala Val Ile Leu Ala Leu Leu Leu Phe Phe Ser Arg Thr Asp Ser Gly 

            180                 185                 190         

Ser Ser Ala Val Val Ala Asp Ala Val Gly Ala Asn Ser Ser Pro Phe 

        195                 200                 205             

Ser Leu Lys Leu Ala Leu Glu Leu Phe Lys Gln Pro Lys Leu Trp Phe 

    210                 215                 220                 

Leu Ser Leu Tyr Val Val Gly Val Ser Cys Thr Tyr Asp Val Phe Asp 

225                 230                 235                 240 

Gln Gln Phe Ala Asn Phe Phe Thr Ser Phe Phe Ala Ser Gly Glu Gln 

                245                 250                 255     

Gly Thr Arg Val Phe Gly Tyr Val Thr Thr Met Gly Glu Leu Leu Asn 

            260                 265                 270         

Ala Cys Ile Met Phe Phe Ala Pro Phe Ile Val Asn Arg Ile Gly Gly 

        275                 280                 285             

Lys Asn Ala Leu Leu Met Ala Gly Ile Ile Met Ser Val Arg Ile Ile 

    290                 295                 300                 

Gly Ser Ser Phe Ala Thr Thr Ala Gly Glu Val Val Ile Leu Lys Thr 

305                 310                 315                 320 

Leu His Met Phe Glu Ile Pro Phe Leu Ile Val Gly Cys Phe Lys Tyr 

                325                 330                 335     

Ile Thr Ser Gln Phe Glu Val Arg Phe Ser Ala Thr Ile Tyr Leu Val 

            340                 345                 350         

Cys Phe Cys Phe Phe Lys Gln Leu Ala Met Ile Phe Met Ser Val Leu 

        355                 360                 365             

Ala Gly Asn Met Tyr Glu Lys Ile Gly Phe Gln Gly Ala Tyr Leu Val 

    370                 375                 380                 

Leu Gly Leu Ile Ala Leu Val Phe Thr Leu Ile Ser Val Ala Thr Leu 

385                 390                 395                 400 

Ser Gly Ala Gly Pro Leu Ala Val Met Arg Val Ser Gln Gln Lys Ser 

                405                 410                 415     

Ala Ser 

        



<210> 10

<211> 423

<212> PRT

<213> Rouxiella badensis





<220> 

<223> Lactose permease, GenBank ID: WP_084912833.1



<400> 10

Met Tyr Tyr Ile Lys Asn Thr Asn Phe Trp Thr Phe Gly Leu Phe Phe 

1               5                   10                  15      

Phe Phe Tyr Phe Phe Ile Met Gly Ala Tyr Phe Pro Phe Phe Pro Ile 

            20                  25                  30          

Trp Leu His Asp Ile Asn His Ile Asn Gln Ser Asp Thr Gly Ile Ile 

        35                  40                  45              

Phe Ala Ser Ile Ser Phe Phe Ser Leu Val Phe Gln Pro Leu Phe Gly 

    50                  55                  60                  

Leu Leu Ser Asp Lys Leu Gly Leu Lys Lys His Leu Leu Trp Ile Ile 

65                  70                  75                  80  

Thr Gly Met Leu Val Met Phe Ala Pro Phe Phe Ile Tyr Val Phe Gly 

                85                  90                  95      

Pro Leu Leu Lys Thr Asn Ile Leu Leu Gly Ser Ile Val Gly Gly Val 

            100                 105                 110         

Tyr Leu Gly Phe Ile Tyr Asn Gly Gly Ala Pro Ala Ile Glu Ala Tyr 

        115                 120                 125             

Ile Glu Lys Val Ser Arg Arg Ser Ser Phe Glu Phe Gly Arg Ala Arg 

    130                 135                 140                 

Met Phe Gly Cys Val Gly Trp Ala Ile Cys Ala Ser Val Val Gly Ile 

145                 150                 155                 160 

Met Phe Thr Ile Asn Ser Gln Phe Val Phe Trp Leu Gly Ser Gly Cys 

                165                 170                 175     

Ala Val Ile Leu Ala Val Leu Leu Leu Val Ala Lys Pro Ala Val Gly 

            180                 185                 190         

Ala Thr Ala Lys Val Ala Asn Glu Leu Gly Ala Asn Ser Lys Pro Phe 

        195                 200                 205             

Ser Leu Arg Leu Ala Ala Glu Leu Leu Lys Asp Lys Lys Leu Trp Phe 

    210                 215                 220                 

Leu Gly Leu Tyr Val Val Gly Val Ser Cys Thr Tyr Glu Val Phe Asp 

225                 230                 235                 240 

Gln Gln Phe Ala Asn Phe Phe Thr Ser Phe Phe Ser Ser Ala Asp Glu 

                245                 250                 255     

Gly Thr Arg Val Phe Gly Tyr Ile Thr Thr Met Gly Glu Leu Leu Asn 

            260                 265                 270         

Ala Leu Val Met Phe Phe Ala Pro Leu Ile Val Asn Arg Ile Gly Gly 

        275                 280                 285             

Lys Asn Ala Leu Leu Leu Ala Gly Ile Ile Met Ser Val Arg Ile Ile 

    290                 295                 300                 

Gly Ser Ser Phe Ala Ser Thr Pro Val Glu Val Val Val Leu Lys Thr 

305                 310                 315                 320 

Leu His Met Phe Glu Val Pro Phe Leu Ile Val Gly Cys Phe Lys Tyr 

                325                 330                 335     

Ile Thr Ser Val Phe Glu Val Arg Phe Ser Ala Thr Ile Tyr Leu Val 

            340                 345                 350         

Cys Phe Cys Phe Phe Lys Gln Ile Ala Met Ile Phe Met Ser Val Phe 

        355                 360                 365             

Ala Gly Asp Met Tyr Gly Lys Ile Gly Phe His Gly Thr Tyr Leu Ile 

    370                 375                 380                 

Leu Gly Leu Ile Ala Leu Ala Phe Thr Leu Leu Ser Val Phe Thr Leu 

385                 390                 395                 400 

Ser Gly Arg Gly Pro Leu Gln Ala Phe Lys Pro Val Ala Arg Thr Val 

                405                 410                 415     

Glu Lys Pro Val Ser Asn Val 

            420             



<210> 11

<211> 417

<212> PRT

<213> Serratia marcescens





<220> 

<223> Lactose permease, GenBank ID: WP_103826752.1



<400> 11

Met Tyr Tyr Leu Lys Asn Thr Asn Phe Trp Met Phe Gly Leu Phe Phe 

1               5                   10                  15      

Phe Phe Tyr Phe Phe Phe Met Gly Ala Tyr Phe Pro Phe Phe Pro Ile 

            20                  25                  30          

Trp Leu His Asp Ile Asn His Ile Ser Lys Ser Asp Thr Gly Ile Ile 

        35                  40                  45              

Phe Ala Ala Ile Ser Leu Phe Ser Leu Leu Phe Gln Pro Leu Phe Gly 

    50                  55                  60                  

Leu Leu Ser Asp Lys Leu Gly Leu Arg Lys Tyr Leu Leu Trp Ile Ile 

65                  70                  75                  80  

Thr Gly Met Leu Val Met Phe Ala Pro Phe Phe Ile Phe Ile Phe Gly 

                85                  90                  95      

Pro Leu Leu Gln Tyr Asn Ile Leu Val Gly Ser Ile Val Gly Gly Ile 

            100                 105                 110         

Tyr Leu Gly Phe Cys Phe Asn Ala Gly Ala Pro Ala Val Glu Ala Phe 

        115                 120                 125             

Ile Glu Lys Val Ser Arg Arg Ser Asn Phe Glu Tyr Gly Arg Ala Arg 

    130                 135                 140                 

Met Phe Gly Cys Val Gly Trp Ala Leu Cys Ala Ser Ile Val Gly Ile 

145                 150                 155                 160 

Met Phe Thr Ile Asn Asn Gln Phe Val Phe Trp Leu Gly Ser Gly Cys 

                165                 170                 175     

Ala Phe Ile Leu Ala Val Leu Leu Phe Phe Ala Lys Thr Asp Ala Pro 

            180                 185                 190         

Ser Ser Ala Thr Val Ala Asn Ala Val Gly Ala Asn His Ser Ala Phe 

        195                 200                 205             

Ser Leu Lys Leu Ala Leu Glu Leu Phe Arg Gln Pro Lys Leu Trp Phe 

    210                 215                 220                 

Leu Ser Leu Tyr Val Ile Gly Val Ser Cys Thr Tyr Asp Val Phe Asp 

225                 230                 235                 240 

Gln Gln Phe Ala Asn Phe Phe Thr Ser Phe Phe Ala Thr Gly Glu Gln 

                245                 250                 255     

Gly Thr Arg Val Phe Gly Tyr Val Thr Thr Met Gly Glu Leu Leu Asn 

            260                 265                 270         

Ala Ser Ile Met Phe Phe Ala Pro Leu Ile Ile Asn Arg Ile Gly Gly 

        275                 280                 285             

Lys Asn Ala Leu Leu Leu Ala Gly Thr Ile Met Ser Val Arg Ile Ile 

    290                 295                 300                 

Gly Ser Ser Phe Ala Thr Ser Ala Leu Glu Val Val Ile Leu Lys Thr 

305                 310                 315                 320 

Leu His Met Phe Glu Val Pro Phe Leu Leu Val Gly Cys Phe Lys Tyr 

                325                 330                 335     

Ile Thr Ser Gln Phe Glu Val Arg Phe Ser Ala Thr Ile Tyr Leu Val 

            340                 345                 350         

Cys Phe Cys Phe Phe Lys Gln Leu Ala Met Ile Phe Met Ser Ile Leu 

        355                 360                 365             

Ala Gly Asn Met Tyr Glu Ser Ile Gly Phe Gln Gly Ala Tyr Leu Val 

    370                 375                 380                 

Leu Gly Leu Val Ala Leu Gly Phe Thr Leu Ile Ser Val Phe Thr Leu 

385                 390                 395                 400 

Ser Gly Pro Gly Pro Leu Ser Leu Leu Arg Arg Gln Val Asn Glu Val 

                405                 410                 415     

Ala 

    



<210> 12

<211> 420

<212> PRT

<213> Serratia fonticola





<220> 

<223> Lactose permease, GenBank ID: WP_021804673.1



<400> 12

Met Tyr Tyr Leu Lys Asn Thr Asn Phe Trp Met Phe Gly Leu Phe Phe 

1               5                   10                  15      

Phe Phe Tyr Phe Phe Ile Met Gly Ala Tyr Phe Pro Phe Phe Pro Ile 

            20                  25                  30          

Trp Leu His Asp Ile Asn Gln Val Ser Lys Ser Asp Thr Gly Ile Ile 

        35                  40                  45              

Phe Ala Ser Ile Ser Phe Phe Ser Leu Leu Phe Gln Pro Ile Phe Gly 

    50                  55                  60                  

Leu Leu Ser Asp Lys Leu Gly Leu Arg Lys His Leu Leu Trp Ile Ile 

65                  70                  75                  80  

Thr Gly Met Leu Ile Leu Phe Ala Pro Phe Phe Ile Tyr Val Phe Gly 

                85                  90                  95      

Pro Leu Leu Arg Thr Asn Ile Val Leu Gly Ser Ile Ala Gly Gly Ile 

            100                 105                 110         

Tyr Leu Gly Phe Ile Tyr Asn Gly Gly Ala Pro Ala Ile Glu Ala Tyr 

        115                 120                 125             

Ile Glu Lys Val Ser Arg Arg Ser Ser Phe Glu Phe Gly Arg Ala Arg 

    130                 135                 140                 

Leu Phe Gly Cys Val Gly Trp Ala Leu Cys Ala Ser Val Val Gly Ile 

145                 150                 155                 160 

Met Phe Thr Ile Asn Asn Glu Phe Val Phe Trp Leu Gly Ser Gly Cys 

                165                 170                 175     

Ala Val Ile Leu Ala Ile Leu Leu Leu Ile Ala Lys Pro Glu Ala Gly 

            180                 185                 190         

Ser Thr Ala Gln Val Ala Asp Lys Leu Gly Ala Asn Ser Lys Pro Phe 

        195                 200                 205             

Asn Leu Arg Leu Ala Phe Glu Leu Leu Lys Asp Ser Lys Leu Trp Phe 

    210                 215                 220                 

Leu Ala Leu Tyr Val Val Gly Val Ser Cys Thr Tyr Asp Val Phe Asp 

225                 230                 235                 240 

Gln Gln Phe Ala Asn Phe Phe Thr Ser Phe Phe Ser Ser Ser Glu Gln 

                245                 250                 255     

Gly Thr Arg Val Phe Gly Tyr Val Thr Thr Met Gly Glu Leu Leu Asn 

            260                 265                 270         

Ala Ser Ile Met Phe Phe Ala Pro Leu Ile Val Asn Arg Ile Gly Gly 

        275                 280                 285             

Lys Asn Ala Leu Leu Leu Ala Gly Leu Ile Met Ser Ile Arg Ile Ile 

    290                 295                 300                 

Gly Ser Ser Phe Ala Ser Thr Pro Val Glu Val Val Ile Leu Lys Thr 

305                 310                 315                 320 

Leu His Met Phe Glu Ile Pro Phe Leu Ile Val Gly Cys Phe Lys Tyr 

                325                 330                 335     

Ile Thr Ser Val Phe Glu Val Arg Phe Ser Ala Thr Ile Tyr Leu Val 

            340                 345                 350         

Cys Phe Cys Phe Phe Lys Gln Leu Ala Ile Met Phe Met Ser Val Phe 

        355                 360                 365             

Ala Gly Asn Met Tyr Glu Lys Val Gly Phe His Gly Thr Tyr Leu Ile 

    370                 375                 380                 

Leu Gly Leu Ile Ala Leu Ser Phe Thr Leu Ile Ser Val Phe Thr Leu 

385                 390                 395                 400 

Ser Gly Arg Gly Pro Leu Ser Ala Phe Lys Pro Glu Pro Glu Ala Val 

                405                 410                 415     

Arg Asn Met Ala 

            420 



<210> 13

<211> 423

<212> PRT

<213> Rouxiella silvae





<220> 

<223> Lactose permease, GenBank ID: WP_084984472.1



<400> 13

Met Asn Tyr Met Arg Asn Thr Asn Phe Trp Thr Phe Gly Leu Phe Phe 

1               5                   10                  15      

Phe Phe Tyr Phe Phe Ile Met Gly Ala Tyr Phe Pro Phe Phe Pro Ile 

            20                  25                  30          

Trp Leu His Asp Ile Asn His Ile Asn Gln Ser Asp Thr Gly Ile Ile 

        35                  40                  45              

Phe Ala Cys Ile Ser Phe Phe Ser Leu Val Phe Gln Pro Leu Phe Gly 

    50                  55                  60                  

Leu Leu Ser Asp Lys Leu Gly Leu Lys Lys His Leu Leu Trp Ile Ile 

65                  70                  75                  80  

Thr Gly Met Leu Val Leu Phe Ala Pro Phe Phe Ile Tyr Val Phe Gly 

                85                  90                  95      

Pro Leu Leu Gln Thr Asn Ile Leu Leu Gly Ser Ile Val Gly Gly Ile 

            100                 105                 110         

Tyr Leu Gly Phe Ile Tyr Asn Gly Gly Ala Pro Ala Ile Glu Ala Tyr 

        115                 120                 125             

Ile Glu Lys Val Ser Arg Arg Ser Ser Phe Glu Phe Gly Arg Ala Arg 

    130                 135                 140                 

Met Phe Gly Cys Val Gly Trp Ala Ile Cys Ala Ser Val Val Gly Ile 

145                 150                 155                 160 

Met Phe Thr Ile Asn Asn Gln Phe Val Phe Trp Leu Gly Ser Gly Cys 

                165                 170                 175     

Ala Val Ile Leu Ala Ile Leu Leu Leu Ile Ala Lys Pro Ala Val Gly 

            180                 185                 190         

Ala Ser Ala Lys Val Ala Asn Glu Leu Gly Ala Asn Ser Lys Pro Phe 

        195                 200                 205             

Asn Leu Arg Leu Ala Ala Glu Leu Leu Lys Asp Lys Lys Leu Trp Phe 

    210                 215                 220                 

Leu Gly Leu Tyr Val Val Gly Val Ser Cys Thr Tyr Glu Val Phe Asp 

225                 230                 235                 240 

Gln Gln Phe Ala Asn Phe Phe Thr Ser Phe Phe Ser Ser Ala Asp Gln 

                245                 250                 255     

Gly Thr Arg Val Phe Gly Tyr Ile Thr Thr Leu Gly Glu Leu Leu Asn 

            260                 265                 270         

Ala Phe Ile Met Phe Phe Ala Pro Ala Ile Val Asn Arg Ile Gly Gly 

        275                 280                 285             

Lys Asn Ala Leu Leu Leu Ala Gly Ala Ile Met Ser Val Arg Ile Ile 

    290                 295                 300                 

Gly Ser Ser Phe Ala Ser Thr Pro Val Glu Val Val Leu Leu Lys Thr 

305                 310                 315                 320 

Leu His Met Phe Glu Ile Pro Phe Leu Ile Val Gly Cys Phe Lys Tyr 

                325                 330                 335     

Ile Thr Ser Val Phe Glu Val Arg Phe Ser Ala Thr Ile Tyr Leu Val 

            340                 345                 350         

Cys Phe Cys Phe Phe Lys Gln Ile Ala Met Ile Phe Met Ser Ile Phe 

        355                 360                 365             

Ala Gly Glu Met Tyr Gly Lys Val Gly Phe His Gly Thr Tyr Leu Ile 

    370                 375                 380                 

Leu Gly Leu Ile Ala Leu Ala Phe Thr Leu Val Ser Ile Phe Thr Leu 

385                 390                 395                 400 

Thr Gly Arg Gly Pro Leu Gln Ala Phe Lys Pro Ser Gln Arg Val Ser 

                405                 410                 415     

Glu Thr Arg Pro Val Ser Pro 

            420             



<210> 14

<211> 412

<212> PRT

<213> Aeromonas





<220> 

<223> Lactose permease, GenBank ID: WP_199428647.1



<400> 14

Met Phe Gly Ala Ile Phe Phe Leu Tyr Phe Phe Ile Met Gly Ala Tyr 

1               5                   10                  15      

Phe Pro Phe Phe Pro Ile Trp Leu His Asp Val Asn Gly Ile Ser Lys 

            20                  25                  30          

Ala Glu Thr Gly Ile Val Phe Gly Thr Ile Ser Leu Phe Ala Leu Leu 

        35                  40                  45              

Phe Gln Pro Val Phe Gly Leu Val Ser Asp Lys Leu Gly Leu Lys Lys 

    50                  55                  60                  

His Leu Leu Trp Ile Ile Ile Gly Leu Leu Val Phe Phe Ala Pro Phe 

65                  70                  75                  80  

Phe Leu Tyr Val Leu Ala Pro Leu Leu Lys Leu Asn Ile Tyr Leu Gly 

                85                  90                  95      

Ser Ile Val Gly Gly Met Tyr Ile Gly Phe Val Phe Ala Gly Gly Ala 

            100                 105                 110         

Pro Ala Ile Glu Ala Tyr Val Glu Lys Val Ser Arg Ser Ser Gln Phe 

        115                 120                 125             

Glu Phe Gly Arg Ala Arg Met Phe Gly Ala Ile Gly Trp Ala Leu Cys 

    130                 135                 140                 

Ala Ser Ile Val Gly Ile Met Phe Thr Ile Asn Asn Glu Phe Val Phe 

145                 150                 155                 160 

Trp Met Gly Ser Ala Phe Ala Val Val Met Ala Val Leu Phe Tyr Phe 

                165                 170                 175     

Ile Asn Pro Gly Arg Gly Ser Thr Ser Glu Val Leu Asp Ser Ile Gly 

            180                 185                 190         

Ala Asn Gln Lys Gly Phe Ser Ile Lys Leu Ala Met Arg Leu Leu Lys 

        195                 200                 205             

Gln Ala Lys Phe Trp Phe Phe Thr Leu Tyr Val Ile Gly Val Ala Cys 

    210                 215                 220                 

Thr Tyr Asp Val Phe Asp Gln Gln Phe Ala Asn Phe Phe Thr Ala Phe 

225                 230                 235                 240 

Phe Asp Thr Lys Glu Glu Gly Thr Arg Tyr Phe Gly Tyr Val Thr Thr 

                245                 250                 255     

Met Gly Glu Leu Leu Asn Ala Ser Ile Met Phe Phe Ala Pro Leu Ile 

            260                 265                 270         

Val Asn Arg Ile Gly Gly Lys Asn Ala Leu Leu Ile Ala Gly Thr Ile 

        275                 280                 285             

Met Ser Ile Arg Ile Ile Gly Ser Ser Phe Ala Glu Thr Val Ser His 

    290                 295                 300                 

Val Ile Leu Leu Lys Thr Leu His Met Leu Glu Val Pro Phe Leu Leu 

305                 310                 315                 320 

Val Gly Thr Phe Lys Tyr Ile Thr Ser Glu Phe Asp Val Arg Phe Ser 

                325                 330                 335     

Ala Thr Ile Trp Leu Ile Gly Tyr Gln Phe Phe Lys Gln Leu Ala Thr 

            340                 345                 350         

Met Phe Met Ser Val Trp Ala Gly Ser Met Tyr Glu Val Met Gly Phe 

        355                 360                 365             

Arg Glu Thr Tyr Leu Val Leu Gly Ile Ile Ala Ala Thr Phe Thr Phe 

    370                 375                 380                 

Ile Ser Ile Phe Thr Leu Ser Gly Met Gly Pro Leu Gly Leu Leu Lys 

385                 390                 395                 400 

Gly Arg Thr Met Val Ser Lys Lys Thr Ala Ser Tyr 

                405                 410         



<210> 15

<211> 425

<212> PRT

<213> Yersinia pestis





<220> 

<223> Lactose permease, GenBank ID: WP_046596210.1



<400> 15

Met Lys Ile Ser Lys Thr Gly Asn Tyr Thr Ile Leu Phe Thr Val Ser 

1               5                   10                  15      

Phe Phe Ile Met Ala Ser Tyr Tyr Pro Phe Phe Pro Ile Trp Leu His 

            20                  25                  30          

Asp Ile Asn Asn Leu Ser Lys Thr Asp Thr Gly Ile Val Phe Gly Ser 

        35                  40                  45              

Ile Ser Leu Phe Ala Leu Ala Phe Gln Pro Ile Met Gly Pro Leu Ser 

    50                  55                  60                  

Asp Lys Leu Gly Leu Arg Lys Thr Leu Met Trp Ile Ile Val Gly Leu 

65                  70                  75                  80  

Leu Val Leu Phe Ala Pro Phe Phe Ile Tyr Val Phe Ser Pro Leu Leu 

                85                  90                  95      

Lys Tyr Asn Ile Phe Ile Gly Ala Ile Val Gly Gly Cys Tyr Leu Gly 

            100                 105                 110         

Phe Val Phe Thr Gly Gly Ser His Ala Ile Glu Ala Tyr Ile Glu Lys 

        115                 120                 125             

Val Ser Arg His Ser Asn Phe Glu Tyr Gly Arg Val Arg Met Phe Gly 

    130                 135                 140                 

Cys Ile Gly Trp Ala Leu Cys Ala Thr Val Val Gly Ile Leu Tyr Thr 

145                 150                 155                 160 

Val Asn Asn Gln Leu Ile Phe Trp Met Ala Ser Gly Cys Ala Leu Ile 

                165                 170                 175     

Leu Ala Val Leu Leu Phe Phe Ala Arg Pro Asp Arg Gln Ser Thr Ala 

            180                 185                 190         

Phe Val Val Asp Thr Leu Gly Ala Asn Lys Ala Val Phe Asn Leu Lys 

        195                 200                 205             

Asn Ala Leu Ala Leu Leu Arg Lys Arg Glu Leu Trp Phe Phe Val Met 

    210                 215                 220                 

Tyr Ile Val Gly Val Ala Cys Ile Tyr Asp Val Phe Asp Gln Gln Phe 

225                 230                 235                 240 

Ala Asn Phe Phe Thr Ser Phe Phe Ala Thr Lys Gln Gln Gly Thr Glu 

                245                 250                 255     

Ile Phe Gly Phe Val Thr Thr Gly Gly Glu Ile Leu Asn Ala Thr Val 

            260                 265                 270         

Met Phe Phe Ala Pro Val Ile Ile Ala Arg Ile Gly Ser Lys Asn Ala 

        275                 280                 285             

Leu Leu Leu Ala Gly Thr Ile Met Ser Val Arg Ile Leu Gly Ser Ala 

    290                 295                 300                 

Phe Ala Thr Thr Ala Thr Gln Val Val Phe Leu Lys Met Leu His Met 

305                 310                 315                 320 

Phe Glu Val Pro Phe Leu Leu Val Gly Ser Phe Lys Tyr Ile Thr Gln 

                325                 330                 335     

Val Phe Glu Val Arg Phe Ser Ala Thr Val Tyr Leu Ile Gly Phe Cys 

            340                 345                 350         

Phe Ser Lys Gln Leu Ser Met Met Phe Met Ser Val Phe Ala Gly Arg 

        355                 360                 365             

Met Tyr Gly Ser Met Gly Tyr Gln Asp Thr Tyr Met Val Leu Gly Val 

    370                 375                 380                 

Ile Val Leu Ser Phe Thr Leu Ile Ser Ala Phe Thr Leu Ser Gly Arg 

385                 390                 395                 400 

Ser Ala Val Ala Asn Leu Ala Ser Arg Leu Lys Glu Asp Pro Ser Val 

                405                 410                 415     

Thr Pro Pro Val Ser Gln Pro Gln Ser 

            420                 425 



<210> 16

<211> 587

<212> PRT

<213> Kluyveromyces lactis





<220> 

<223> Lactose permease, GenBank ID: XP_452193.1



<400> 16

Met Ala Asp His Ser Ser Ser Ser Ser Ser Leu Gln Lys Lys Pro Ile 

1               5                   10                  15      

Asn Thr Ile Glu His Lys Asp Thr Leu Gly Asn Asp Arg Asp His Lys 

            20                  25                  30          

Glu Ala Leu Asn Ser Asp Asn Asp Asn Thr Ser Gly Leu Lys Ile Asn 

        35                  40                  45              

Gly Val Pro Ile Glu Asp Ala Arg Glu Glu Val Leu Leu Pro Gly Tyr 

    50                  55                  60                  

Leu Ser Lys Gln Tyr Tyr Lys Leu Tyr Gly Leu Cys Phe Ile Thr Tyr 

65                  70                  75                  80  

Leu Cys Ala Thr Met Gln Gly Tyr Asp Gly Ala Leu Met Gly Ser Ile 

                85                  90                  95      

Tyr Thr Glu Asp Ala Tyr Leu Lys Tyr Tyr His Leu Asp Ile Asn Ser 

            100                 105                 110         

Ser Ser Gly Thr Gly Leu Val Phe Ser Ile Phe Asn Val Gly Gln Ile 

        115                 120                 125             

Cys Gly Ala Phe Phe Val Pro Leu Met Asp Trp Lys Gly Arg Lys Pro 

    130                 135                 140                 

Ala Ile Leu Ile Gly Cys Leu Gly Val Val Ile Gly Ala Ile Ile Ser 

145                 150                 155                 160 

Ser Leu Thr Thr Thr Lys Ser Ala Leu Ile Gly Gly Arg Trp Phe Val 

                165                 170                 175     

Ala Phe Phe Ala Thr Ile Ala Asn Ala Ala Ala Pro Thr Tyr Cys Ala 

            180                 185                 190         

Glu Val Ala Pro Ala His Leu Arg Gly Lys Val Ala Gly Leu Tyr Asn 

        195                 200                 205             

Thr Leu Trp Ser Val Gly Ser Ile Val Ala Ala Phe Ser Thr Tyr Gly 

    210                 215                 220                 

Thr Asn Lys Asn Phe Pro Asn Ser Ser Lys Ala Phe Lys Ile Pro Leu 

225                 230                 235                 240 

Tyr Leu Gln Met Met Phe Pro Gly Leu Val Cys Ile Phe Gly Trp Leu 

                245                 250                 255     

Ile Pro Glu Ser Pro Arg Trp Leu Val Gly Val Gly Arg Glu Glu Glu 

            260                 265                 270         

Ala Arg Glu Phe Ile Ile Lys Tyr His Leu Asn Gly Asp Arg Thr His 

        275                 280                 285             

Pro Leu Leu Asp Met Glu Met Ala Glu Ile Ile Glu Ser Phe His Gly 

    290                 295                 300                 

Thr Asp Leu Ser Asn Pro Leu Glu Met Leu Asp Val Arg Ser Leu Phe 

305                 310                 315                 320 

Arg Thr Arg Ser Asp Arg Tyr Arg Ala Met Leu Val Ile Leu Met Ala 

                325                 330                 335     

Trp Phe Gly Gln Phe Ser Gly Asn Asn Val Cys Ser Tyr Tyr Leu Pro 

            340                 345                 350         

Thr Met Leu Arg Asn Val Gly Met Lys Ser Val Ser Leu Asn Val Leu 

        355                 360                 365             

Met Asn Gly Val Tyr Ser Ile Val Thr Trp Ile Ser Ser Ile Cys Gly 

    370                 375                 380                 

Ala Phe Phe Ile Asp Lys Ile Gly Arg Arg Glu Gly Phe Leu Gly Ser 

385                 390                 395                 400 

Ile Ser Gly Ala Ala Leu Ala Leu Thr Gly Leu Ser Ile Cys Thr Ala 

                405                 410                 415     

Arg Tyr Glu Lys Thr Lys Lys Lys Ser Ala Ser Asn Gly Ala Leu Val 

            420                 425                 430         

Phe Ile Tyr Leu Phe Gly Gly Ile Phe Ser Phe Ala Phe Thr Pro Met 

        435                 440                 445             

Gln Ser Met Tyr Ser Thr Glu Val Ser Thr Asn Leu Thr Arg Ser Lys 

    450                 455                 460                 

Ala Gln Leu Leu Asn Phe Val Val Ser Gly Val Ala Gln Phe Val Asn 

465                 470                 475                 480 

Gln Phe Ala Thr Pro Lys Ala Met Lys Asn Ile Lys Tyr Trp Phe Tyr 

                485                 490                 495     

Val Phe Tyr Val Phe Phe Asp Ile Phe Glu Phe Ile Val Ile Tyr Phe 

            500                 505                 510         

Phe Phe Val Glu Thr Lys Gly Arg Ser Leu Glu Glu Leu Glu Val Val 

        515                 520                 525             

Phe Glu Ala Pro Asn Pro Arg Lys Ala Ser Val Asp Gln Ala Phe Leu 

    530                 535                 540                 

Ala Gln Val Arg Ala Thr Leu Val Gln Arg Asn Asp Val Arg Val Ala 

545                 550                 555                 560 

Asn Ala Gln Asn Leu Lys Glu Gln Glu Pro Leu Lys Ser Asp Ala Asp 

                565                 570                 575     

His Val Glu Lys Leu Ser Glu Ala Glu Ser Val 

            580                 585         



<210> 17

<211> 262

<212> PRT

<213> Chromobacterium violaceum





<220> 

<223> cvb3galT, Î²-1,3-galactosyltransferase, GeneBank ID:

      WP_080969100.1



<400> 17

Met Asp Thr Ile Met Ile Lys Arg Pro Leu Val Ser Val Ile Leu Pro 

1               5                   10                  15      

Val Asn Lys Asn Asn Pro His Leu Glu Glu Ala Ile Gln Ser Ile Lys 

            20                  25                  30          

Asn Gln Thr Tyr Lys Glu Leu Glu Leu Ile Ile Ile Ala Asn Asn Cys 

        35                  40                  45              

Glu Asp Asn Phe Tyr Ser Leu Leu Leu Lys Tyr Gln Asp Gln Lys Thr 

    50                  55                  60                  

Lys Ile Ile Arg Thr Ser Ile Lys Tyr Leu Pro Phe Ser Leu Asn Leu 

65                  70                  75                  80  

Gly Val His Leu Ser Gln Gly Glu Tyr Ile Ala Arg Met Asp Ser Asp 

                85                  90                  95      

Asp Ile Ser Val Leu Asp Arg Ile Glu Lys Gln Val Lys Arg Phe Leu 

            100                 105                 110         

Asn Thr Pro Glu Leu Ser Ile Leu Gly Ser Asn Val Glu Tyr Ile Asn 

        115                 120                 125             

Glu Ala Ser Glu Ser Ile Gly Tyr Ser Asn Tyr Pro Leu Asp His Ser 

    130                 135                 140                 

Ser Ile Val Asn Ser Phe Pro Phe Arg Cys Asn Leu Ala His Pro Thr 

145                 150                 155                 160 

Ile Met Val Lys Lys Glu Val Ile Thr Thr Leu Gly Gly Tyr Met Tyr 

                165                 170                 175     

Gly Ser Leu Ser Glu Asp Tyr Asp Leu Trp Ile Arg Ala Ser Arg His 

            180                 185                 190         

Gly Asn Phe Lys Phe Ser Asn Ile Asp Glu Pro Leu Leu Lys Tyr Arg 

        195                 200                 205             

Ile His Lys Gly Gln Ala Thr Asn Lys Ser Asn Ala Tyr Asn Ile Phe 

    210                 215                 220                 

Ala Phe Asp Ser Ser Leu Lys Ile Arg Glu Phe Leu Leu Asn Gly Asn 

225                 230                 235                 240 

Val Gln Tyr Leu Leu Gly Ala Ala Arg Gly Phe Phe Ala Phe Leu Tyr 

                245                 250                 255     

Val Arg Phe Ile Lys Lys 

            260         



<210> 18

<211> 439

<212> PRT

<213> Artificial Sequence





<220> 

<223> galTK, Î²-1,3-galactosyltransferase, homologous to GeneBank ID:

      BD182026.1



<400> 18

Met Ile Ser Val Tyr Ile Ile Ser Leu Lys Glu Ser Gln Arg Arg Leu 

1               5                   10                  15      

Asp Thr Glu Lys Leu Val Leu Glu Ser Asn Glu Lys Phe Lys Gly Arg 

            20                  25                  30          

Cys Val Phe Gln Ile Phe Asp Ala Ile Ser Pro Lys His Glu Asp Phe 

        35                  40                  45              

Glu Lys Phe Val Gln Glu Leu Tyr Asp Ser Ser Ser Leu Leu Lys Ser 

    50                  55                  60                  

Asp Trp Phe His Ser Asp Tyr Cys Tyr Gln Glu Leu Leu Pro Gln Glu 

65                  70                  75                  80  

Phe Gly Cys Tyr Leu Ser His Tyr Leu Leu Trp Lys Glu Cys Val Lys 

                85                  90                  95      

Leu Asn Gln Pro Val Val Ile Leu Glu Asp Asp Val Ala Leu Glu Ser 

            100                 105                 110         

Asn Phe Met Gln Ala Leu Glu Asp Cys Leu Lys Ser Pro Phe Asp Phe 

        115                 120                 125             

Val Arg Leu Tyr Gly His Tyr Trp Gly Gly His Lys Thr Asn Leu Cys 

    130                 135                 140                 

Ala Leu Pro Val Tyr Thr Glu Thr Glu Glu Ala Glu Ala Ser Ile Glu 

145                 150                 155                 160 

Lys Thr Pro Ile Glu Asn Tyr Glu Val Thr Ser Pro Pro Pro Pro Asn 

                165                 170                 175     

Pro Thr Arg Asp Thr Gln Gln Asp Phe Ile Thr Glu Thr Gln Gln Asp 

            180                 185                 190         

Pro Lys Glu Leu Ser Glu Pro Cys Lys Ile Ala Pro Gln Lys Ile Ser 

        195                 200                 205             

Phe Asn Gln Val Val Phe Lys Lys Ile Lys Arg Lys Leu Asn Arg Phe 

    210                 215                 220                 

Ile Gly Ser Ile Leu Ala Arg Thr Glu Val Tyr Lys Asn Ile Val Ala 

225                 230                 235                 240 

Lys Tyr Asp Asp Leu Thr Thr Lys Tyr Asp Asp Leu Thr Thr Lys Tyr 

                245                 250                 255     

Asp Asp Leu Thr Thr Lys Tyr Asp Asp Leu Thr Thr Lys Tyr Asp Asp 

            260                 265                 270         

Leu Asn Lys Asn Ile Ala Glu Lys Tyr Asp Glu Leu Met Gly Lys Tyr 

        275                 280                 285             

Glu Ser Leu Leu Ala Lys Glu Val Asn Ile Lys Glu Thr Phe Trp Glu 

    290                 295                 300                 

Ser Arg Ala Asp Ser Glu Lys Glu Ala Leu Phe Leu Asp His Phe Tyr 

305                 310                 315                 320 

Leu Thr Ser Val Tyr Val Ala Thr Thr Ala Gly Tyr Tyr Leu Thr Pro 

                325                 330                 335     

Lys Gly Ala Lys Thr Phe Ile Glu Ala Thr Glu Arg Phe Lys Ile Ile 

            340                 345                 350         

Glu Pro Val Asp Met Phe Ile Asn Asn Pro Thr Tyr His Asp Ile Ala 

        355                 360                 365             

Asn Phe Thr Tyr Val Pro Cys Pro Val Ser Leu Asn Lys His Ala Phe 

    370                 375                 380                 

Asn Ser Thr Ile Gln Asn Ala Lys Lys Pro Asp Ile Ser Leu Lys Pro 

385                 390                 395                 400 

Pro Lys Lys Ser Tyr Phe Asp Asn Leu Phe Tyr His Lys Phe Asn Ala 

                405                 410                 415     

Arg Lys Cys Leu Lys Ala Phe Asn Lys Tyr Ser Lys Gln Tyr Ala Pro 

            420                 425                 430         

Leu Lys Thr Pro Lys Glu Val 

        435                 



<210> 19

<211> 273

<212> PRT

<213> Helicobacter pylori





<220> 

<223> Î²-1,4-gal-transferase, GalT, GenBank ID: WP_001262061.1



<400> 19

Met Arg Val Phe Ala Ile Ser Leu Asn Gln Lys Val Cys Asp Thr Phe 

1               5                   10                  15      

Gly Leu Val Phe Arg Asp Thr Thr Thr Leu Leu Asn Ser Ile Asn Ala 

            20                  25                  30          

Thr His His Gln Ala Gln Ile Phe Asp Ala Ile Tyr Ser Lys Thr Phe 

        35                  40                  45              

Glu Gly Gly Leu His Pro Leu Val Lys Lys His Leu His Pro Tyr Phe 

    50                  55                  60                  

Ile Thr Gln Asn Ile Lys Asp Met Gly Ile Thr Thr Asn Leu Ile Ser 

65                  70                  75                  80  

Glu Val Ser Lys Phe Tyr Tyr Ala Leu Lys Tyr His Ala Lys Phe Met 

                85                  90                  95      

Ser Leu Gly Glu Leu Gly Cys Tyr Ala Ser His Tyr Ser Leu Trp Glu 

            100                 105                 110         

Lys Cys Ile Glu Leu Asn Glu Ala Ile Cys Ile Leu Glu Asp Asp Ile 

        115                 120                 125             

Thr Leu Lys Glu Asp Phe Lys Glu Gly Leu Asp Phe Leu Glu Lys His 

    130                 135                 140                 

Ile Gln Glu Leu Gly Tyr Ile Arg Leu Met His Leu Leu Tyr Asp Ala 

145                 150                 155                 160 

Ser Val Lys Ser Glu Pro Leu Ser His Lys Asn His Glu Ile Gln Glu 

                165                 170                 175     

Arg Val Gly Ile Ile Lys Ala Tyr Ser Glu Gly Val Gly Thr Gln Gly 

            180                 185                 190         

Tyr Val Ile Thr Pro Lys Ile Ala Lys Val Phe Leu Lys Cys Ser Arg 

        195                 200                 205             

Lys Trp Val Val Pro Val Asp Thr Ile Met Asp Ala Thr Phe Ile His 

    210                 215                 220                 

Gly Val Lys Asn Leu Val Leu Gln Pro Phe Val Ile Ala Asp Asp Glu 

225                 230                 235                 240 

Gln Ile Ser Thr Ile Ala Arg Lys Glu Glu Pro Tyr Ser Pro Lys Ile 

                245                 250                 255     

Ala Leu Met Arg Glu Leu His Phe Lys Tyr Leu Lys Tyr Trp Gln Phe 

            260                 265                 270         

Val 

    



<210> 20

<211> 332

<212> PRT

<213> Neisseria meningitidis





<220> 

<223> lgtA, Î²-1,3-N-acetyl-glucosaminyltransferase, GeneBank ID:

      WP_033911473.1



<400> 20

Met Gln Pro Leu Val Ser Val Leu Ile Cys Ala Tyr Asn Val Glu Lys 

1               5                   10                  15      

Tyr Phe Ala Gln Ser Leu Ala Ala Val Val Asn Gln Thr Trp Arg Asn 

            20                  25                  30          

Leu Glu Ile Leu Ile Val Asp Asp Gly Ser Thr Asp Gly Thr Leu Ala 

        35                  40                  45              

Ile Ala Lys Asp Phe Gln Lys Arg Asp Ser Arg Ile Lys Ile Leu Ala 

    50                  55                  60                  

Gln Ala Gln Asn Ser Gly Leu Ile Pro Ser Leu Asn Ile Gly Leu Asp 

65                  70                  75                  80  

Glu Leu Ala Lys Ser Gly Met Gly Glu Tyr Ile Ala Arg Thr Asp Ala 

                85                  90                  95      

Asp Asp Ile Ala Ala Pro Asp Trp Ile Glu Lys Ile Val Gly Glu Met 

            100                 105                 110         

Glu Lys Asp Arg Ser Ile Ile Ala Met Gly Ala Trp Leu Glu Val Leu 

        115                 120                 125             

Ser Glu Glu Lys Asp Gly Asn Arg Leu Ala Arg His His Arg His Gly 

    130                 135                 140                 

Lys Ile Trp Lys Lys Pro Thr Arg Pro Glu Asp Ile Ala Asp Phe Phe 

145                 150                 155                 160 

Pro Phe Gly Asn Pro Ile His Asn Asn Thr Met Ile Met Arg Arg Ser 

                165                 170                 175     

Val Ile Asp Gly Gly Leu Arg Tyr Asn Thr Glu Arg Asp Trp Ala Glu 

            180                 185                 190         

Asp Tyr Gln Phe Trp Tyr Asp Val Ser Lys Leu Gly Arg Leu Ala Tyr 

        195                 200                 205             

Tyr Pro Glu Ala Leu Val Lys Tyr Arg Leu His Ala Asn Gln Val Ser 

    210                 215                 220                 

Ser Lys Tyr Ser Ile Arg Gln His Glu Ile Ala Gln Gly Ile Gln Lys 

225                 230                 235                 240 

Thr Ala Arg Asn Asp Phe Leu Gln Ser Met Gly Phe Lys Thr Arg Phe 

                245                 250                 255     

Asp Ser Leu Glu Tyr Arg Gln Ile Lys Ala Val Ala Tyr Glu Leu Leu 

            260                 265                 270         

Glu Lys His Leu Pro Glu Glu Asp Phe Glu Arg Ala Arg Arg Phe Leu 

        275                 280                 285             

Tyr Gln Cys Phe Lys Arg Thr Asp Thr Leu Pro Ala Gly Ala Trp Leu 

    290                 295                 300                 

Asp Phe Ala Ala Asp Gly Arg Met Arg Arg Leu Phe Thr Leu Arg Gln 

305                 310                 315                 320 

Tyr Phe Gly Ile Leu His Arg Leu Leu Lys Asn Arg 

                325                 330         



<210> 21

<211> 334

<212> PRT

<213> Pasteurella multocida





<220> 

<223> pmnagT, Î²-1,3-N-acetylglucosaminyl-transferase, GeneBankID:

      WP_014390683.1



<400> 21

Met Glu Asn Lys Pro Leu Val Ser Val Leu Ile Cys Ala Tyr Asn Val 

1               5                   10                  15      

Glu Lys Tyr Ile Glu Glu Cys Ile Asn Ala Val Ile Asn Gln Thr Tyr 

            20                  25                  30          

Lys Asn Leu Glu Ile Ile Ile Val Asn Asp Gly Ser Ser Asp Asn Thr 

        35                  40                  45              

Tyr Phe Leu Leu Lys Lys Leu Ala Glu Lys Asp Asn Arg Ile Lys Ile 

    50                  55                  60                  

Leu Asn Phe Asn Asn His Ile Gly Ile Ile Ser Ala Leu Asn Glu Gly 

65                  70                  75                  80  

Leu Lys Glu Ile Ala Gly Glu Tyr Ile Ala Arg Thr Asp Ser Asp Asp 

                85                  90                  95      

Ile Thr Lys Pro Asp Trp Ile Glu Lys Ile Leu Thr Cys Met Gln Asn 

            100                 105                 110         

Asp Pro Lys Ile Ile Ala Met Gly Ser Tyr Leu Thr Val Leu Ser Glu 

        115                 120                 125             

Glu Asn Asn Gly Ser Val Leu Ala Asn His His Lys Asn Lys Val Glu 

    130                 135                 140                 

Trp Lys Asn Pro Leu Glu His Lys Asp Ile Val Glu Lys Met Leu Phe 

145                 150                 155                 160 

Gly Asn Pro Ile His Asn Asn Ser Met Val Met Arg Ser Glu Ile Tyr 

                165                 170                 175     

Thr Lys Tyr His Leu Ile Tyr Asp Pro Asp Tyr His Tyr Ala Glu Asp 

            180                 185                 190         

Tyr Lys Phe Trp Leu Glu Val Ser Arg Ile Gly Lys Leu Ala Asn Tyr 

        195                 200                 205             

Pro Glu Ser Leu Val Tyr Tyr Arg Leu His Arg Asn Gln Thr Ser Ser 

    210                 215                 220                 

Ile His Asn Ser Gln Gln Glu Ile Asn Gly Lys Lys Leu Arg Leu Gln 

225                 230                 235                 240 

Ala Leu Asn Tyr Tyr Leu Lys Asp Leu Gly Ile Asp Tyr Gln Leu Pro 

                245                 250                 255     

Glu Lys Phe Leu Phe Lys Asp Ile Ala Leu Leu Gln Glu Ile Phe Tyr 

            260                 265                 270         

Glu Arg Gly Met Phe Arg Glu Asn Ile Ile Arg Arg Ile Ile Tyr Glu 

        275                 280                 285             

Cys Tyr Leu Ser Leu Gly Glu Tyr Asn Tyr Lys Asp Ile Tyr Tyr Phe 

    290                 295                 300                 

Leu Ile Asn Lys Asn Asn Phe Leu Ser Ile Lys Asp Lys Phe Lys Ile 

305                 310                 315                 320 

Ile Lys Lys Tyr Leu Arg Pro Asp Lys Tyr Ser Ser Thr Tyr 

                325                 330                 



<210> 22

<211> 330

<212> PRT

<213> Haemophilus ducreyi





<220> 

<223> HD0466, glycosyltransferase family 2 protein, GeneBank ID:

      WP_010944479.1



<400> 22

Met Thr Thr Leu Val Ser Val Leu Ile Cys Ala Tyr Asn Val Glu Lys 

1               5                   10                  15      

Tyr Ile Asp Glu Cys Leu Asn Ala Val Ile Ala Gln Thr Tyr Lys Asn 

            20                  25                  30          

Leu Glu Ile Ile Val Val Asn Asp Gly Ser Thr Asp Gly Thr Leu Ala 

        35                  40                  45              

Lys Leu Arg Gln Phe Glu Ala Lys Asp Pro Arg Val Lys Ile Ile Asp 

    50                  55                  60                  

Asn Ile Val Asn Gln Gly Thr Ser Lys Ser Leu Asn Ile Gly Ile Gln 

65                  70                  75                  80  

Tyr Cys Gln Gly Glu Ile Ile Ala Arg Thr Asp Ser Asp Asp Ile Val 

                85                  90                  95      

Asp Ile His Trp Ile Glu Thr Leu Met Arg Glu Leu Asp Asn Ser Pro 

            100                 105                 110         

Glu Thr Ile Ala Ile Ser Ala Tyr Leu Glu Phe Leu Ala Glu Lys Gly 

        115                 120                 125             

Asn Gly Ser Lys Leu Ser Arg Ser Arg Lys His Gly Lys Asn Ala Glu 

    130                 135                 140                 

Asn Pro Ile Ser Ser Glu Ala Ile Ser Gln Arg Met Leu Phe Gly Asn 

145                 150                 155                 160 

Pro Val His Asn Asn Val Ala Leu Val Arg Arg Lys Val Phe Ser Glu 

                165                 170                 175     

Tyr Gly Leu Arg Phe Asp Pro Asp Tyr Ile His Ala Glu Asp Tyr Lys 

            180                 185                 190         

Phe Trp Phe Glu Val Ser Lys Leu Gly Lys Met Arg Thr Tyr Pro Lys 

        195                 200                 205             

Ala Leu Val Lys Tyr Arg Leu His Ala Thr Gln Val Ser Ser Ala Tyr 

    210                 215                 220                 

Asn Gln Lys Gln Arg Ser Ile Ala Lys Lys Ile Lys Arg Glu Ala Ile 

225                 230                 235                 240 

Ser His Tyr Leu Gln Gln Tyr Gly Ile Gln Leu Pro Glu Lys Leu Thr 

                245                 250                 255     

Ile His Asp Leu Phe Ser Ile Phe Ser Pro Gln Ile Glu Leu Ser Leu 

            260                 265                 270         

Thr Val Ala Asn Lys Gln Glu Leu Phe Trp Ser Leu Ala Thr Ser Leu 

        275                 280                 285             

Ser Glu Tyr His Phe Arg Asp Leu Leu Lys Ile Tyr Ser Leu Asp Ile 

    290                 295                 300                 

Phe His Gln Leu Ser Phe Lys Tyr Lys Lys Arg Ile Phe Arg Lys Phe 

305                 310                 315                 320 

Leu Leu Pro Asn Arg Tyr Pro Ser Val Ile 

                325                 330 



<210> 23

<211> 302

<212> PRT

<213> Artificial Sequence





<220> 

<223> FutC (Î±-1,2-fucosyl-transferase)



<400> 23

Met Ala Phe Lys Val Val Gln Ile Cys Gly Gly Leu Gly Asn Gln Met 

1               5                   10                  15      

Phe Gln Tyr Ala Phe Ala Lys Ser Leu Gln Lys His Ser Asn Thr Pro 

            20                  25                  30          

Val Leu Leu Asp Ile Thr Ser Phe Asp Trp Ser Asp Arg Lys Met Gln 

        35                  40                  45              

Leu Glu Leu Phe Pro Ile Asp Leu Pro Tyr Ala Ser Ala Lys Glu Ile 

    50                  55                  60                  

Ala Ile Ala Lys Met Gln His Leu Pro Lys Leu Val Arg Asp Ala Leu 

65                  70                  75                  80  

Lys Cys Met Gly Phe Asp Arg Val Ser Gln Glu Ile Val Phe Glu Tyr 

                85                  90                  95      

Glu Pro Lys Leu Leu Lys Pro Ser Arg Leu Thr Tyr Phe Phe Gly Tyr 

            100                 105                 110         

Phe Gln Asp Pro Arg Tyr Phe Asp Ala Ile Ser Pro Leu Ile Lys Gln 

        115                 120                 125             

Thr Phe Thr Leu Pro Pro Pro Pro Glu Asn Asn Lys Asn Asn Asn Lys 

    130                 135                 140                 

Lys Glu Glu Glu Tyr Gln Cys Lys Leu Ser Leu Ile Leu Ala Ala Lys 

145                 150                 155                 160 

Asn Ser Val Phe Val His Ile Arg Arg Gly Asp Tyr Val Gly Ile Gly 

                165                 170                 175     

Cys Gln Leu Gly Ile Asp Tyr Gln Lys Lys Ala Leu Glu Tyr Met Ala 

            180                 185                 190         

Lys Arg Val Pro Asn Met Glu Leu Phe Val Phe Cys Glu Asp Leu Glu 

        195                 200                 205             

Phe Thr Gln Asn Leu Asp Leu Gly Tyr Pro Phe Met Asp Met Thr Thr 

    210                 215                 220                 

Arg Asp Lys Glu Glu Glu Ala Tyr Trp Asp Met Leu Leu Met Gln Ser 

225                 230                 235                 240 

Cys Gln His Gly Ile Ile Ala Asn Ser Thr Tyr Ser Trp Trp Ala Ala 

                245                 250                 255     

Tyr Leu Ile Glu Asn Pro Glu Lys Ile Ile Ile Gly Pro Lys His Trp 

            260                 265                 270         

Leu Phe Gly His Glu Asn Ile Leu Cys Lys Glu Trp Val Lys Ile Glu 

        275                 280                 285             

Ser His Phe Glu Val Lys Ser Gln Lys Tyr Asn Ala Leu Gly 

    290                 295                 300         



<210> 24

<211> 292

<212> PRT

<213> Sulfuriflexus mobilis





<220> 

<223> Smob, Î±-1,2-fucosyltransferase, GeneBank ID: WP_126455392.1



<400> 24

Met Ile Ile Ser Gln Ile Ile Gly Gly Leu Gly Asn Gln Met Phe Gln 

1               5                   10                  15      

Tyr Ala Ala Gly Arg Ala Leu Ser Leu Val Arg Gly Gln Pro Leu Leu 

            20                  25                  30          

Leu Asp Val Thr Gly Phe Ala Gly Tyr Gly Leu His Gln Gly Phe Glu 

        35                  40                  45              

Leu Gln Arg Val Phe Asp Cys Pro Ile Gly Ile Ala Thr Glu Glu Asp 

    50                  55                  60                  

Val Arg Gly Ile Leu Gly Trp Gln Phe Ser Ala Gly Ile Arg Arg Ile 

65                  70                  75                  80  

Val Ala Arg Pro Gly Met Ala Ala Phe Arg Arg Lys Gly Phe Ile Val 

                85                  90                  95      

Glu Pro His Phe His Tyr Trp Pro Glu Ile Lys Asn Val Pro Arg Asp 

            100                 105                 110         

Cys Tyr Leu Leu Gly Tyr Trp Gln Ser Glu Arg Tyr Phe Arg Ala Ala 

        115                 120                 125             

Thr Ala Asp Ile Arg Ala Asp Phe Ser Phe Lys Ser Pro Leu Val Asn 

    130                 135                 140                 

Arg Asn Ala Glu Thr Ala Ala Gln Ile Asp Gln Val Asn Ala Ile Ser 

145                 150                 155                 160 

Leu His Met Arg Arg Gly Asp Tyr Val Asn Asn Pro Lys Thr Ser Ala 

                165                 170                 175     

Thr His Gly Leu Cys Ser Leu Asp Tyr Tyr Gln Ala Ala Ile Lys Phe 

            180                 185                 190         

Val Ser Glu Arg Val Glu Glu Pro Phe Phe Phe Ile Phe Ser Asp Asp 

        195                 200                 205             

Ile Ala Trp Val Lys Ala Asn Leu Lys Leu Asp Phe Pro Cys Gln Tyr 

    210                 215                 220                 

Val Asp His Asn His Gly Ala Glu Ser Phe Asn Asp Met His Leu Met 

225                 230                 235                 240 

Ser Leu Cys Gln His His Ile Ile Ala Asn Ser Ser Phe Ser Trp Trp 

                245                 250                 255     

Gly Ala Trp Leu Asn Ser Asp Pro Lys Lys Ile Val Leu Ala Pro Lys 

            260                 265                 270         

Lys Trp Phe Ala Asn Lys Asn Asn Ile Lys Asp Leu Phe Pro Pro Gly 

        275                 280                 285             

Trp Val Ser Leu 

    290         



<210> 25

<211> 292

<212> PRT

<213> Methylobacter tundripaludum





<220> 

<223> Mtun, Î±-1,2-fucosyltransferase, GeneBank ID: WP_031437198.1



<400> 25

Met Val Ile Thr His Leu Ile Gly Gly Leu Gly Asn Gln Met Phe Gln 

1               5                   10                  15      

Tyr Ala Ala Gly Arg Ala Val Ser Leu Glu Arg Gly Val Ser Leu Ser 

            20                  25                  30          

Leu Asp Ile Ser Gly Phe Ala Asn Tyr Gly Leu His Gln Gly Phe Glu 

        35                  40                  45              

Leu Gln Arg Ile Phe Asn Cys Thr Ala Glu Ile Ala Asn Glu Ala Asp 

    50                  55                  60                  

Val Arg Gly Ile Leu Gly Trp Gln Ser Ser Pro Arg Ile Arg Gln Leu 

65                  70                  75                  80  

Leu Ser Arg Gln Asn Met Ala Ile Phe Arg Arg Glu Gly Phe Val Val 

                85                  90                  95      

Glu Pro His Phe His Tyr Trp Gln Gly Ile Lys Ser Val Pro Arg Asp 

            100                 105                 110         

Cys Tyr Leu Thr Gly Tyr Trp Gln Ser Glu Gln Tyr Phe Leu Glu Ala 

        115                 120                 125             

Ala Ala Gln Ile Arg Ala Asp Phe Thr Phe Lys Leu Pro Leu Asp Asn 

    130                 135                 140                 

Gln Asn Ile Glu Leu Ala Lys Gln Ile Asn Ala Val Asn Ala Val Ser 

145                 150                 155                 160 

Leu His Val Arg Arg Gly Asp Tyr Ala Asn Thr Pro Glu Thr Thr Ala 

                165                 170                 175     

Thr His Gly Leu Cys Ser Leu Asp Tyr Tyr Arg Val Ala Ile Arg His 

            180                 185                 190         

Ile Ala Glu Gln Val Gln Gln Pro His Phe Phe Val Phe Ser Asp Asp 

        195                 200                 205             

Ile Ala Trp Val Lys Asn Asn Leu Ser Ile Asp Phe Pro Cys Gln Tyr 

    210                 215                 220                 

Val Asp His Asn Gln Gly Ala Glu Ser Tyr Asn Asp Met Arg Leu Met 

225                 230                 235                 240 

Ser Met Cys Arg His His Ile Ile Ala Asn Ser Ser Phe Ser Trp Trp 

                245                 250                 255     

Gly Ala Trp Leu Asn Pro Asn Val Asn Lys Ile Val Val Ala Pro Ser 

            260                 265                 270         

Arg Trp Phe Ala Lys Gln Thr Asp Val Arg Asp Leu Leu Pro Gln Gly 

        275                 280                 285             

Trp Ile Lys Gln 

    290         



<210> 26

<211> 425

<212> PRT

<213> Helicobacter pylori





<220> 

<223> FutA, Î±-1,3-fucosyl-transferase, GenBank ID: NP_207177.1



<400> 26

Met Phe Gln Pro Leu Leu Asp Ala Phe Ile Glu Ser Ala Ser Ile Glu 

1               5                   10                  15      

Lys Met Ala Ser Lys Ser Pro Pro Pro Pro Leu Lys Ile Ala Val Ala 

            20                  25                  30          

Asn Trp Trp Gly Asp Glu Glu Ile Lys Glu Phe Lys Lys Ser Val Leu 

        35                  40                  45              

Tyr Phe Ile Leu Ser Gln Arg Tyr Ala Ile Thr Leu His Gln Asn Pro 

    50                  55                  60                  

Asn Glu Phe Ser Asp Leu Val Phe Ser Asn Pro Leu Gly Ala Ala Arg 

65                  70                  75                  80  

Lys Ile Leu Ser Tyr Gln Asn Thr Lys Arg Val Phe Tyr Thr Gly Glu 

                85                  90                  95      

Asn Glu Ser Pro Asn Phe Asn Leu Phe Asp Tyr Ala Ile Gly Phe Asp 

            100                 105                 110         

Glu Leu Asp Phe Asn Asp Arg Tyr Leu Arg Met Pro Leu Tyr Tyr Ala 

        115                 120                 125             

His Leu His Tyr Lys Ala Glu Leu Val Asn Asp Thr Thr Ala Pro Tyr 

    130                 135                 140                 

Lys Leu Lys Asp Asn Ser Leu Tyr Ala Leu Lys Lys Pro Ser His His 

145                 150                 155                 160 

Phe Lys Glu Asn His Pro Asn Leu Cys Ala Val Val Asn Asp Glu Ser 

                165                 170                 175     

Asp Leu Leu Lys Arg Gly Phe Ala Ser Phe Val Ala Ser Asn Ala Asn 

            180                 185                 190         

Ala Pro Met Arg Asn Ala Phe Tyr Asp Ala Leu Asn Ser Ile Glu Pro 

        195                 200                 205             

Val Thr Gly Gly Gly Ser Val Arg Asn Thr Leu Gly Tyr Lys Val Gly 

    210                 215                 220                 

Asn Lys Ser Glu Phe Leu Ser Gln Tyr Lys Phe Asn Leu Cys Phe Glu 

225                 230                 235                 240 

Asn Ser Gln Gly Tyr Gly Tyr Val Thr Glu Lys Ile Leu Asp Ala Tyr 

                245                 250                 255     

Phe Ser His Thr Ile Pro Ile Tyr Trp Gly Ser Pro Ser Val Ala Lys 

            260                 265                 270         

Asp Phe Asn Pro Lys Ser Phe Val Asn Val His Asp Phe Asn Asn Phe 

        275                 280                 285             

Asp Glu Ala Ile Asp Tyr Ile Lys Tyr Leu His Thr His Pro Asn Ala 

    290                 295                 300                 

Tyr Leu Asp Met Leu Tyr Glu Asn Pro Leu Asn Thr Leu Asp Gly Lys 

305                 310                 315                 320 

Ala Tyr Phe Tyr Gln Asp Leu Ser Phe Lys Lys Ile Leu Asp Phe Phe 

                325                 330                 335     

Lys Thr Ile Leu Glu Asn Asp Thr Ile Tyr His Lys Phe Ser Thr Ser 

            340                 345                 350         

Phe Met Trp Glu Tyr Asp Leu His Lys Pro Leu Val Ser Ile Asp Asp 

        355                 360                 365             

Leu Arg Val Asn Tyr Asp Asp Leu Arg Val Asn Tyr Asp Arg Leu Leu 

    370                 375                 380                 

Gln Asn Ala Ser Pro Leu Leu Glu Leu Ser Gln Asn Thr Thr Phe Lys 

385                 390                 395                 400 

Ile Tyr Arg Lys Ala Tyr Gln Lys Ser Leu Pro Leu Leu Arg Ala Val 

                405                 410                 415     

Arg Lys Leu Val Lys Lys Leu Gly Leu 

            420                 425 



<210> 27

<211> 478

<212> PRT

<213> Helicobacter pylori NCTC 11639





<220> 

<223> FucT, alpha1,3-fucosyltransferase, GenBank ID NO: AAB81031.1



<400> 27

Met Phe Gln Pro Leu Leu Asp Ala Tyr Val Glu Ser Ala Ser Ile Glu 

1               5                   10                  15      

Lys Met Ala Ser Lys Ser Pro Pro Pro Leu Lys Ile Ala Val Ala Asn 

            20                  25                  30          

Trp Trp Gly Asp Glu Glu Ile Lys Glu Phe Lys Asn Ser Val Leu Tyr 

        35                  40                  45              

Phe Ile Leu Ser Gln Arg Tyr Thr Ile Thr Leu His Gln Asn Pro Asn 

    50                  55                  60                  

Glu Phe Ser Asp Leu Val Phe Gly Asn Pro Leu Gly Ser Ala Arg Lys 

65                  70                  75                  80  

Ile Leu Ser Tyr Gln Asn Ala Lys Arg Val Phe Tyr Thr Gly Glu Asn 

                85                  90                  95      

Glu Ser Pro Asn Phe Asn Leu Phe Asp Tyr Ala Ile Gly Phe Asp Glu 

            100                 105                 110         

Leu Asp Phe Asn Asp Arg Tyr Leu Arg Met Pro Leu Tyr Tyr Asp Arg 

        115                 120                 125             

Leu His His Lys Ala Glu Ser Val Asn Asp Thr Thr Ala Pro Tyr Lys 

    130                 135                 140                 

Leu Lys Asp Asn Ser Leu Tyr Ala Leu Lys Lys Pro Ser His Cys Phe 

145                 150                 155                 160 

Lys Glu Lys His Pro Asn Leu Cys Ala Val Val Asn Asp Glu Ser Asp 

                165                 170                 175     

Pro Leu Lys Arg Gly Phe Ala Ser Phe Val Ala Ser Asn Pro Asn Ala 

            180                 185                 190         

Pro Ile Arg Asn Ala Phe Tyr Asp Ala Leu Asn Ser Ile Glu Pro Val 

        195                 200                 205             

Thr Gly Gly Gly Ser Val Arg Asn Thr Leu Gly Tyr Asn Val Lys Asn 

    210                 215                 220                 

Lys Asn Glu Phe Leu Ser Gln Tyr Lys Phe Asn Leu Cys Phe Glu Asn 

225                 230                 235                 240 

Thr Gln Gly Tyr Gly Tyr Val Thr Glu Lys Ile Ile Asp Ala Tyr Phe 

                245                 250                 255     

Ser His Thr Ile Pro Ile Tyr Trp Gly Ser Pro Ser Val Ala Lys Asp 

            260                 265                 270         

Phe Asn Pro Lys Ser Phe Val Asn Val His Asp Phe Lys Asn Phe Asp 

        275                 280                 285             

Glu Ala Ile Asp Tyr Ile Lys Tyr Leu His Thr His Lys Asn Ala Tyr 

    290                 295                 300                 

Leu Asp Met Leu Tyr Glu Asn Pro Leu Asn Thr Leu Asp Gly Lys Ala 

305                 310                 315                 320 

Tyr Phe Tyr Gln Asn Leu Ser Phe Lys Lys Ile Leu Ala Phe Phe Lys 

                325                 330                 335     

Thr Ile Leu Glu Asn Asp Thr Ile Tyr His Asp Asn Pro Phe Ile Phe 

            340                 345                 350         

Cys Arg Asp Leu Asn Glu Pro Leu Val Thr Ile Asp Asp Leu Arg Val 

        355                 360                 365             

Asn Tyr Asp Asp Leu Arg Val Asn Tyr Asp Asp Leu Arg Ile Asn Tyr 

    370                 375                 380                 

Asp Asp Leu Arg Val Asn Tyr Asp Asp Leu Arg Ile Asn Tyr Asp Asp 

385                 390                 395                 400 

Leu Arg Val Asn Tyr Asp Asp Leu Arg Val Asn Tyr Asp Asp Leu Arg 

                405                 410                 415     

Ile Asn Tyr Asp Asp Leu Arg Val Asn Tyr Asp Asp Leu Arg Val Asn 

            420                 425                 430         

Tyr Glu Arg Leu Leu Ser Lys Ala Thr Pro Leu Leu Glu Leu Ser Gln 

        435                 440                 445             

Asn Thr Thr Ser Lys Ile Tyr Arg Lys Ala Tyr Gln Lys Ser Leu Pro 

    450                 455                 460                 

Leu Leu Arg Ala Ile Arg Arg Trp Val Lys Lys Leu Gly Leu 

465                 470                 475             



<210> 28

<211> 432

<212> PRT

<213> Helicobacter pylori





<220> 

<223> FucTIII, alpha-1,4 fucosyltransferase, GenBank ID: AAR88243.1



<400> 28

Met Phe Gln Pro Leu Leu Asp Ala Tyr Ile Asp Ser Thr Arg Leu Asp 

1               5                   10                  15      

Glu Thr Asp Tyr Lys Pro Pro Leu Lys Ile Ala Val Ala Asn Trp Trp 

            20                  25                  30          

Gly Gly Val Glu Glu Phe Lys Lys Ser Thr Leu Tyr Phe Ile Leu Ser 

        35                  40                  45              

Gln Arg Tyr Thr Ile Thr Leu His Arg Asn Pro Asp Lys Pro Ala Asp 

    50                  55                  60                  

Ile Val Phe Gly Asn Pro Leu Gly Ser Ala Arg Lys Ile Leu Ser Tyr 

65                  70                  75                  80  

Gln Asn Ala Lys Arg Val Phe Tyr Thr Gly Glu Asn Glu Val Pro Asn 

                85                  90                  95      

Phe Asn Leu Phe Asp Tyr Ala Ile Gly Phe Asp Glu Leu Asp Phe Asn 

            100                 105                 110         

Asp Arg Tyr Leu Arg Met Pro Leu Tyr Tyr Ala His Leu His Tyr Glu 

        115                 120                 125             

Ala Glu Leu Val Asn Asp Thr Thr Ser Pro Tyr Lys Ile Lys Asp Asn 

    130                 135                 140                 

Ser Leu Tyr Ala Leu Lys Lys Pro Ser His His Phe Lys Glu Asn His 

145                 150                 155                 160 

Pro Asn Leu Cys Ala Val Val Asn Asn Glu Ser Asp Pro Leu Lys Arg 

                165                 170                 175     

Gly Phe Ala Ser Phe Val Ala Ser Asn Pro Asn Ala Pro Lys Arg Asn 

            180                 185                 190         

Ala Phe Tyr Asp Ala Leu Asn Ser Ile Glu Pro Val Thr Gly Gly Gly 

        195                 200                 205             

Ser Val Lys Asn Thr Leu Gly Tyr Asn Val Lys Asn Lys Asn Glu Phe 

    210                 215                 220                 

Leu Ser Gln Tyr Lys Phe Asn Leu Cys Phe Glu Asn Ser Gln Gly Tyr 

225                 230                 235                 240 

Gly Tyr Val Thr Glu Lys Ile Leu Asp Ala Tyr Phe Ser His Thr Ile 

                245                 250                 255     

Pro Ile Tyr Trp Gly Ser Pro Ser Val Ala Lys Asp Phe Asn Pro Lys 

            260                 265                 270         

Ser Phe Val Asn Val His Asp Phe Asn Asn Phe Asp Glu Ala Ile Asp 

        275                 280                 285             

His Val Arg Tyr Leu His Thr His Pro Asn Ala Tyr Leu Asp Met Leu 

    290                 295                 300                 

Tyr Glu Asn Pro Leu Asn Thr Leu Asp Gly Lys Ala Tyr Phe Tyr Gln 

305                 310                 315                 320 

Asn Leu Ser Phe Lys Lys Ile Leu Asp Phe Phe Lys Thr Ile Leu Glu 

                325                 330                 335     

Asn Asp Thr Ile Tyr His Cys Asp Ala His Asn Tyr Ser Ala Leu His 

            340                 345                 350         

Arg Asp Leu Asn Glu Pro Leu Val Ser Ile Asp Asp Leu Arg Ile Asn 

        355                 360                 365             

Tyr Asp Asp Leu Arg Ile Asn Tyr Asp Asp Leu Arg Ile Asn Tyr Asp 

    370                 375                 380                 

Asp Leu Arg Ile Asn Tyr Glu Arg Leu Leu Gln Asn Ala Ser Pro Leu 

385                 390                 395                 400 

Leu Glu Leu Ser Gln Asn Thr Ser Phe Lys Ile Tyr Arg Lys Ala Tyr 

                405                 410                 415     

Gln Lys Ser Leu Pro Leu Leu Arg Ala Ile Arg Arg Trp Val Lys Lys 

            420                 425                 430         







<210> 29

<211> 483

<212> PRT

<213> Artificial Sequence





<220> 

<223> Pd2 (Î±-1,3-sialyl-transferase)



<400> 29

Met Cys Asn Ser Asp Asn Thr Ser Leu Lys Glu Thr Val Ser Ser Asn 

1               5                   10                  15      

Ser Ala Asp Val Val Glu Thr Glu Thr Tyr Gln Leu Thr Pro Ile Asp 

            20                  25                  30          

Ala Pro Ser Ser Phe Leu Ser His Ser Trp Glu Gln Thr Cys Gly Thr 

        35                  40                  45              

Pro Ile Leu Asn Glu Ser Asp Lys Gln Ala Ile Ser Phe Asp Phe Val 

    50                  55                  60                  

Ala Pro Glu Leu Lys Gln Asp Glu Lys Tyr Cys Phe Thr Phe Lys Gly 

65                  70                  75                  80  

Ile Thr Gly Asp His Arg Tyr Ile Thr Asn Thr Thr Leu Thr Val Val 

                85                  90                  95      

Ala Pro Thr Leu Glu Val Tyr Ile Asp His Ala Ser Leu Pro Ser Leu 

            100                 105                 110         

Gln Gln Leu Ile His Ile Ile Gln Ala Lys Asp Glu Tyr Pro Ser Asn 

        115                 120                 125             

Gln Arg Phe Val Ser Trp Lys Arg Val Thr Val Asp Ala Asp Asn Ala 

    130                 135                 140                 

Asn Lys Leu Asn Ile His Thr Tyr Pro Leu Lys Gly Asn Asn Thr Ser 

145                 150                 155                 160 

Pro Glu Met Val Ala Ala Ile Asp Glu Tyr Ala Gln Ser Lys Asn Arg 

                165                 170                 175     

Leu Asn Ile Glu Phe Tyr Thr Asn Thr Ala His Val Phe Asn Asn Leu 

            180                 185                 190         

Pro Pro Ile Ile Gln Pro Leu Tyr Asn Asn Glu Lys Val Lys Ile Ser 

        195                 200                 205             

His Ile Ser Leu Tyr Asp Asp Gly Ser Ser Glu Tyr Val Ser Leu Tyr 

    210                 215                 220                 

Gln Trp Lys Asp Thr Pro Asn Lys Ile Glu Thr Leu Glu Gly Glu Val 

225                 230                 235                 240 

Ser Leu Leu Ala Asn Tyr Leu Ala Gly Thr Ser Pro Asp Ala Pro Lys 

                245                 250                 255     

Gly Met Gly Asn Arg Tyr Asn Trp His Lys Leu Tyr Asp Thr Asp Tyr 

            260                 265                 270         

Tyr Phe Leu Arg Glu Asp Tyr Leu Asp Val Glu Ala Asn Leu His Asp 

        275                 280                 285             

Leu Arg Asp Tyr Leu Gly Ser Ser Ala Lys Gln Met Pro Trp Asp Glu 

    290                 295                 300                 

Phe Ala Lys Leu Ser Asp Ser Gln Gln Thr Leu Phe Leu Asp Ile Val 

305                 310                 315                 320 

Gly Phe Asp Lys Glu Gln Leu Gln Gln Gln Tyr Ser Gln Ser Pro Leu 

                325                 330                 335     

Pro Asn Phe Ile Phe Thr Gly Thr Thr Thr Trp Ala Gly Gly Glu Thr 

            340                 345                 350         

Lys Glu Tyr Tyr Ala Gln Gln Gln Val Asn Val Ile Asn Asn Ala Ile 

        355                 360                 365             

Asn Glu Thr Ser Pro Tyr Tyr Leu Gly Lys Asp Tyr Asp Leu Phe Phe 

    370                 375                 380                 

Lys Gly His Pro Ala Gly Gly Val Ile Asn Asp Ile Ile Leu Gly Ser 

385                 390                 395                 400 

Phe Pro Asp Met Ile Asn Ile Pro Ala Lys Ile Ser Phe Glu Val Leu 

                405                 410                 415     

Met Met Thr Asp Met Leu Pro Asp Thr Val Ala Gly Ile Ala Ser Ser 

            420                 425                 430         

Leu Tyr Phe Thr Ile Pro Ala Asp Lys Val Asn Phe Ile Val Phe Thr 

        435                 440                 445             

Ser Ser Asp Thr Ile Thr Asp Arg Glu Glu Ala Leu Lys Ser Pro Leu 

    450                 455                 460                 

Val Gln Val Met Leu Thr Leu Gly Ile Val Lys Glu Lys Asp Val Leu 

465                 470                 475                 480 

Phe Trp Ala 

            



<210> 30

<211> 342

<212> PRT

<213> Artificial Sequence





<220> 

<223> Nst (Î±-1,3-sialyl-transferase)



<400> 30

Met Glu Arg Asn Ala Val Ser Leu Leu Lys Glu Lys Leu Phe Asn Glu 

1               5                   10                  15      

Glu Gly Glu Pro Val Asn Leu Ile Phe Cys Tyr Thr Ile Leu Gln Met 

            20                  25                  30          

Lys Val Ala Glu Arg Ile Met Ala Gln His Pro Gly Glu Arg Phe Tyr 

        35                  40                  45              

Val Val Leu Met Ser Glu Asn Arg Asn Glu Lys Tyr Asp Tyr Tyr Phe 

    50                  55                  60                  

Asn Gln Ile Lys Asp Lys Ala Glu Arg Ala Tyr Phe Phe His Leu Pro 

65                  70                  75                  80  

Tyr Gly Leu Asn Lys Ser Phe Asn Phe Ile Pro Thr Met Ala Glu Leu 

                85                  90                  95      

Lys Val Lys Ser Met Leu Leu Pro Lys Val Lys Arg Ile Tyr Leu Ala 

            100                 105                 110         

Ser Leu Glu Lys Val Ser Ile Ala Ala Phe Leu Ser Thr Tyr Pro Asp 

        115                 120                 125             

Ala Glu Ile Lys Thr Phe Asp Asp Gly Thr Gly Asn Leu Ile Gln Ser 

    130                 135                 140                 

Ser Ser Tyr Leu Gly Asp Glu Phe Ser Val Asn Gly Thr Ile Lys Arg 

145                 150                 155                 160 

Asn Phe Ala Arg Met Met Ile Gly Asp Trp Ser Ile Ala Lys Thr Arg 

                165                 170                 175     

Asn Ala Ser Asp Glu His Tyr Thr Ile Phe Lys Gly Leu Lys Asn Ile 

            180                 185                 190         

Met Asp Asp Gly Arg Arg Lys Met Thr Tyr Leu Pro Leu Phe Asp Ala 

        195                 200                 205             

Ser Glu Leu Lys Thr Gly Asp Glu Thr Gly Gly Thr Val Arg Ile Leu 

    210                 215                 220                 

Leu Gly Ser Pro Asp Lys Glu Met Lys Glu Ile Ser Glu Lys Ala Ala 

225                 230                 235                 240 

Lys Asn Phe Lys Ile Gln Tyr Val Ala Pro His Pro Arg Gln Thr Tyr 

                245                 250                 255     

Gly Leu Ser Gly Val Thr Thr Leu Asn Ser Pro Tyr Val Ile Glu Asp 

            260                 265                 270         

Tyr Ile Leu Arg Glu Ile Lys Lys Asn Pro His Thr Arg Tyr Glu Ile 

        275                 280                 285             

Tyr Thr Phe Phe Ser Gly Ala Ala Leu Thr Met Lys Asp Phe Pro Asn 

    290                 295                 300                 

Val His Val Tyr Ala Leu Lys Pro Ala Ser Leu Pro Glu Asp Tyr Trp 

305                 310                 315                 320 

Leu Lys Pro Val Tyr Ala Leu Phe Thr Gln Ser Gly Ile Pro Ile Leu 

                325                 330                 335     

Thr Phe Asp Asp Lys Asn 

            340         



<210> 31

<211> 373

<212> PRT

<213> Escherichia coli K-12





<220> 

<223> gmd, GDP-mannose 4,6-dehydratas, GenBank ID: NP_416557.1



<400> 31

Met Ser Lys Val Ala Leu Ile Thr Gly Val Thr Gly Gln Asp Gly Ser 

1               5                   10                  15      

Tyr Leu Ala Glu Phe Leu Leu Glu Lys Gly Tyr Glu Val His Gly Ile 

            20                  25                  30          

Lys Arg Arg Ala Ser Ser Phe Asn Thr Glu Arg Val Asp His Ile Tyr 

        35                  40                  45              

Gln Asp Pro His Thr Cys Asn Pro Lys Phe His Leu His Tyr Gly Asp 

    50                  55                  60                  

Leu Ser Asp Thr Ser Asn Leu Thr Arg Ile Leu Arg Glu Val Gln Pro 

65                  70                  75                  80  

Asp Glu Val Tyr Asn Leu Gly Ala Met Ser His Val Ala Val Ser Phe 

                85                  90                  95      

Glu Ser Pro Glu Tyr Thr Ala Asp Val Asp Ala Met Gly Thr Leu Arg 

            100                 105                 110         

Leu Leu Glu Ala Ile Arg Phe Leu Gly Leu Glu Lys Lys Thr Arg Phe 

        115                 120                 125             

Tyr Gln Ala Ser Thr Ser Glu Leu Tyr Gly Leu Val Gln Glu Ile Pro 

    130                 135                 140                 

Gln Lys Glu Thr Thr Pro Phe Tyr Pro Arg Ser Pro Tyr Ala Val Ala 

145                 150                 155                 160 

Lys Leu Tyr Ala Tyr Trp Ile Thr Val Asn Tyr Arg Glu Ser Tyr Gly 

                165                 170                 175     

Met Tyr Ala Cys Asn Gly Ile Leu Phe Asn His Glu Ser Pro Arg Arg 

            180                 185                 190         

Gly Glu Thr Phe Val Thr Arg Lys Ile Thr Arg Ala Ile Ala Asn Ile 

        195                 200                 205             

Ala Gln Gly Leu Glu Ser Cys Leu Tyr Leu Gly Asn Met Asp Ser Leu 

    210                 215                 220                 

Arg Asp Trp Gly His Ala Lys Asp Tyr Val Lys Met Gln Trp Met Met 

225                 230                 235                 240 

Leu Gln Gln Glu Gln Pro Glu Asp Phe Val Ile Ala Thr Gly Val Gln 

                245                 250                 255     

Tyr Ser Val Arg Gln Phe Val Glu Met Ala Ala Ala Gln Leu Gly Ile 

            260                 265                 270         

Lys Leu Arg Phe Glu Gly Thr Gly Val Glu Glu Lys Gly Ile Val Val 

        275                 280                 285             

Ser Val Thr Gly His Asp Ala Pro Gly Val Lys Pro Gly Asp Val Ile 

    290                 295                 300                 

Ile Ala Val Asp Pro Arg Tyr Phe Arg Pro Ala Glu Val Glu Thr Leu 

305                 310                 315                 320 

Leu Gly Asp Pro Thr Lys Ala His Glu Lys Leu Gly Trp Lys Pro Glu 

                325                 330                 335     

Ile Thr Leu Arg Glu Met Val Ser Glu Met Val Ala Asn Asp Leu Glu 

            340                 345                 350         

Ala Ala Lys Lys His Ser Leu Leu Lys Ser His Gly Tyr Asp Val Ala 

        355                 360                 365             

Ile Ala Leu Glu Ser 

    370             



<210> 32

<211> 373

<212> PRT

<213> Enterobacteriaceae





<220> 

<223> gmd, GDP-mannose 4,6-dehydratas, GenBank ID: WP_000048190.1



<400> 32

Met Ser Lys Val Ala Leu Ile Thr Gly Val Thr Gly Gln Asp Gly Ser 

1               5                   10                  15      

Tyr Leu Ala Glu Phe Leu Leu Glu Lys Gly Tyr Glu Val His Gly Ile 

            20                  25                  30          

Lys Arg Arg Ala Ser Ser Phe Asn Thr Glu Arg Val Asp His Ile Tyr 

        35                  40                  45              

Gln Asp Pro His Thr Cys Asn Pro Lys Phe His Leu His Tyr Gly Asp 

    50                  55                  60                  

Leu Ser Asp Thr Ser Asn Leu Thr Arg Ile Leu Arg Glu Val Gln Pro 

65                  70                  75                  80  

Asp Glu Val Tyr Asn Leu Gly Ala Met Ser His Val Ala Val Ser Phe 

                85                  90                  95      

Glu Ser Pro Glu Tyr Thr Ala Asp Val Asp Ala Met Gly Thr Leu Arg 

            100                 105                 110         

Leu Leu Glu Ala Ile Arg Phe Leu Gly Leu Glu Lys Lys Thr Arg Phe 

        115                 120                 125             

Tyr Gln Ala Ser Thr Ser Glu Leu Tyr Gly Leu Val Gln Glu Ile Pro 

    130                 135                 140                 

Gln Lys Glu Thr Thr Pro Phe Tyr Pro Arg Ser Pro Tyr Ala Val Ala 

145                 150                 155                 160 

Lys Leu Tyr Ala Tyr Trp Ile Thr Val Asn Tyr Arg Glu Ser Tyr Gly 

                165                 170                 175     

Met Tyr Ala Cys Asn Gly Ile Leu Phe Asn His Glu Ser Pro Arg Arg 

            180                 185                 190         

Gly Glu Thr Phe Val Thr Arg Lys Ile Thr Arg Ala Ile Ala Asn Ile 

        195                 200                 205             

Ala Gln Gly Leu Glu Ser Cys Leu Tyr Leu Gly Asn Met Asp Ser Leu 

    210                 215                 220                 

Arg Asp Trp Gly His Ala Lys Asp Tyr Val Lys Met Gln Trp Met Met 

225                 230                 235                 240 

Leu Gln Gln Glu Gln Pro Glu Asp Phe Val Ile Ala Thr Gly Val Gln 

                245                 250                 255     

Tyr Ser Val Arg Gln Phe Val Glu Met Ala Ala Ala Gln Leu Gly Ile 

            260                 265                 270         

Lys Leu Arg Phe Glu Gly Thr Gly Val Glu Glu Lys Gly Ile Val Val 

        275                 280                 285             

Ser Val Thr Gly His Asp Ala Pro Gly Val Lys Pro Gly Asp Val Ile 

    290                 295                 300                 

Ile Ala Val Asp Pro Arg Tyr Phe Arg Pro Ala Glu Val Glu Thr Leu 

305                 310                 315                 320 

Leu Gly Asp Pro Thr Lys Ala His Glu Lys Leu Gly Trp Lys Pro Glu 

                325                 330                 335     

Ile Thr Leu Arg Glu Met Val Ser Glu Met Val Ala Asn Asp Leu Glu 

            340                 345                 350         

Ala Ala Lys Lys His Ser Leu Leu Lys Ser His Gly Tyr Asp Val Ala 

        355                 360                 365             

Ile Ala Leu Glu Ser 

    370             



<210> 33

<211> 321

<212> PRT

<213> Escherichia coli K-12





<220> 

<223> WcaG, GDP-L-fucose synthase



<400> 33

Met Ser Lys Gln Arg Val Phe Ile Ala Gly His Arg Gly Met Val Gly 

1               5                   10                  15      

Ser Ala Ile Arg Arg Gln Leu Glu Gln Arg Gly Asp Val Glu Leu Val 

            20                  25                  30          

Leu Arg Thr Arg Asp Glu Leu Asn Leu Leu Asp Ser Arg Ala Val His 

        35                  40                  45              

Asp Phe Phe Ala Ser Glu Arg Ile Asp Gln Val Tyr Leu Ala Ala Ala 

    50                  55                  60                  

Lys Val Gly Gly Ile Val Ala Asn Asn Thr Tyr Pro Ala Asp Phe Ile 

65                  70                  75                  80  

Tyr Gln Asn Met Met Ile Glu Ser Asn Ile Ile His Ala Ala His Gln 

                85                  90                  95      

Asn Asp Val Asn Lys Leu Leu Phe Leu Gly Ser Ser Cys Ile Tyr Pro 

            100                 105                 110         

Lys Leu Ala Lys Gln Pro Met Ala Glu Ser Glu Leu Leu Gln Gly Thr 

        115                 120                 125             

Leu Glu Pro Thr Asn Glu Pro Tyr Ala Ile Ala Lys Ile Ala Gly Ile 

    130                 135                 140                 

Lys Leu Cys Glu Ser Tyr Asn Arg Gln Tyr Gly Arg Asp Tyr Arg Ser 

145                 150                 155                 160 

Val Met Pro Thr Asn Leu Tyr Gly Pro His Asp Asn Phe His Pro Ser 

                165                 170                 175     

Asn Ser His Val Ile Pro Ala Leu Leu Arg Arg Phe His Glu Ala Thr 

            180                 185                 190         

Ala Gln Asn Ala Pro Asp Val Val Val Trp Gly Ser Gly Thr Pro Met 

        195                 200                 205             

Arg Glu Phe Leu His Val Asp Asp Met Ala Ala Ala Ser Ile His Val 

    210                 215                 220                 

Met Glu Leu Ala His Glu Val Trp Leu Glu Asn Thr Gln Pro Met Leu 

225                 230                 235                 240 

Ser His Ile Asn Val Gly Thr Gly Val Asp Cys Thr Ile Arg Glu Leu 

                245                 250                 255     

Ala Gln Thr Ile Ala Lys Val Val Gly Tyr Lys Gly Arg Val Val Phe 

            260                 265                 270         

Asp Ala Ser Lys Pro Asp Gly Thr Pro Arg Lys Leu Leu Asp Val Thr 

        275                 280                 285             

Arg Leu His Gln Leu Gly Trp Tyr His Glu Ile Ser Leu Glu Ala Gly 

    290                 295                 300                 

Leu Ala Ser Thr Tyr Gln Trp Phe Leu Glu Asn Gln Asp Arg Phe Arg 

305                 310                 315                 320 

Gly 

    



<210> 34

<211> 321

<212> PRT

<213> Enterobacteriaceae





<220> 

<223> WcaG, GDP-L-fucose synthase, GenBank ID: WP_000043654.1



<400> 34

Met Ser Lys Gln Arg Val Phe Ile Ala Gly His Arg Gly Met Val Gly 

1               5                   10                  15      

Ser Ala Ile Arg Arg Gln Leu Glu Gln Arg Gly Asp Val Glu Leu Val 

            20                  25                  30          

Leu Arg Thr Arg Asp Glu Leu Asn Leu Leu Asp Ser Arg Ala Val His 

        35                  40                  45              

Asp Phe Phe Ala Ser Glu Arg Ile Asp Gln Val Tyr Leu Ala Ala Ala 

    50                  55                  60                  

Lys Val Gly Gly Ile Val Ala Asn Asn Thr Tyr Pro Ala Asp Phe Ile 

65                  70                  75                  80  

Tyr Gln Asn Met Met Ile Glu Ser Asn Ile Ile His Ala Ala His Gln 

                85                  90                  95      

Asn Asp Val Asn Lys Leu Leu Phe Leu Gly Ser Ser Cys Ile Tyr Pro 

            100                 105                 110         

Lys Leu Ala Lys Gln Pro Met Ala Glu Ser Glu Leu Leu Gln Gly Thr 

        115                 120                 125             

Leu Glu Pro Thr Asn Glu Pro Tyr Ala Ile Ala Lys Ile Ala Gly Ile 

    130                 135                 140                 

Lys Leu Cys Glu Ser Tyr Asn Arg Gln Tyr Gly Arg Asp Tyr Arg Ser 

145                 150                 155                 160 

Val Met Pro Thr Asn Leu Tyr Gly Pro His Asp Asn Phe His Pro Ser 

                165                 170                 175     

Asn Ser His Val Ile Pro Ala Leu Leu Arg Arg Phe His Glu Ala Thr 

            180                 185                 190         

Ala Gln Asn Ala Pro Asp Val Val Val Trp Gly Ser Gly Thr Pro Met 

        195                 200                 205             

Arg Glu Phe Leu His Val Asp Asp Met Ala Ala Ala Ser Ile His Val 

    210                 215                 220                 

Met Glu Leu Ala His Glu Val Trp Leu Glu Asn Thr Gln Pro Met Leu 

225                 230                 235                 240 

Ser His Ile Asn Val Gly Thr Gly Val Asp Cys Thr Ile Arg Glu Leu 

                245                 250                 255     

Ala Gln Thr Ile Ala Lys Val Val Gly Tyr Lys Gly Arg Val Val Phe 

            260                 265                 270         

Asp Ala Ser Lys Pro Asp Gly Thr Pro Arg Lys Leu Leu Asp Val Thr 

        275                 280                 285             

Arg Leu His Gln Leu Gly Trp Tyr His Glu Ile Ser Leu Glu Ala Gly 

    290                 295                 300                 

Leu Ala Ser Thr Tyr Gln Trp Phe Leu Glu Asn Gln Asp Arg Phe Arg 

305                 310                 315                 320 

Gly 

    



<210> 35

<211> 159

<212> PRT

<213> Escherichia coli





<220> 

<223> WcaH, GDP-mannose mannosyl hydrolase, GenBank ID: NP_416555.2



<400> 35

Met Phe Leu Arg Gln Glu Asp Phe Ala Thr Val Val Arg Ser Thr Pro 

1               5                   10                  15      

Leu Val Ser Leu Asp Phe Ile Val Glu Asn Ser Arg Gly Glu Phe Leu 

            20                  25                  30          

Leu Gly Lys Arg Thr Asn Arg Pro Ala Gln Gly Tyr Trp Phe Val Pro 

        35                  40                  45              

Gly Gly Arg Val Gln Lys Asp Glu Thr Leu Glu Ala Ala Phe Glu Arg 

    50                  55                  60                  

Leu Thr Met Ala Glu Leu Gly Leu Arg Leu Pro Ile Thr Ala Gly Gln 

65                  70                  75                  80  

Phe Tyr Gly Val Trp Gln His Phe Tyr Asp Asp Asn Phe Ser Gly Thr 

                85                  90                  95      

Asp Phe Thr Thr His Tyr Val Val Leu Gly Phe Arg Phe Arg Val Ser 

            100                 105                 110         

Glu Glu Glu Leu Leu Leu Pro Asp Glu Gln His Asp Asp Tyr Arg Trp 

        115                 120                 125             

Leu Thr Ser Asp Ala Leu Leu Ala Ser Asp Asn Val His Ala Asn Ser 

    130                 135                 140                 

Arg Ala Tyr Phe Leu Ala Glu Lys Arg Thr Gly Val Pro Gly Leu 

145                 150                 155                 



<210> 36

<211> 159

<212> PRT

<213> Escherichia





<220> 

<223> WcaH, GDP-mannose mannosyl hydrolase, GenBank ID: WP_001393539.1



<400> 36

Met Phe Leu Arg Gln Glu Asp Phe Ala Thr Val Val Arg Ser Thr Pro 

1               5                   10                  15      

Leu Val Ser Leu Asp Phe Ile Val Glu Asn Ser Arg Gly Glu Phe Leu 

            20                  25                  30          

Leu Gly Lys Arg Thr Asn Arg Pro Ala Gln Gly Tyr Trp Phe Val Pro 

        35                  40                  45              

Gly Gly Arg Val Gln Lys Asp Glu Thr Leu Glu Ala Ala Phe Glu Arg 

    50                  55                  60                  

Leu Thr Met Ala Glu Leu Gly Leu Arg Leu Pro Ile Thr Ala Gly Gln 

65                  70                  75                  80  

Phe Tyr Gly Val Trp Gln His Phe Tyr Asp Asp Asn Phe Ser Gly Thr 

                85                  90                  95      

Asp Phe Thr Thr His Tyr Val Val Leu Gly Phe Arg Phe Arg Val Ser 

            100                 105                 110         

Glu Glu Glu Leu Leu Leu Pro Asp Glu Gln His Asp Asp Tyr Arg Trp 

        115                 120                 125             

Leu Thr Ser Asp Ala Leu Leu Ala Ser Asp Asn Val His Ala Asn Ser 

    130                 135                 140                 

Arg Ala Tyr Phe Leu Ala Glu Lys Arg Thr Gly Val Pro Gly Leu 

145                 150                 155                 



<210> 37

<211> 407

<212> PRT

<213> Escherichia coli





<220> 

<223> WcaI, colanic acid biosynthesis fucosyltransferase, GenBank ID:

      NP_416554.1



<400> 37

Met Lys Ile Leu Val Tyr Gly Ile Asn Tyr Ser Pro Glu Leu Thr Gly 

1               5                   10                  15      

Ile Gly Lys Tyr Thr Gly Glu Met Val Glu Trp Leu Ala Ala Gln Gly 

            20                  25                  30          

His Glu Val Arg Val Ile Thr Ala Pro Pro Tyr Tyr Pro Gln Trp Gln 

        35                  40                  45              

Val Gly Glu Asn Tyr Ser Ala Trp Arg Tyr Lys Arg Glu Glu Gly Ala 

    50                  55                  60                  

Ala Thr Val Trp Arg Cys Pro Leu Tyr Val Pro Lys Gln Pro Ser Thr 

65                  70                  75                  80  

Leu Lys Arg Leu Leu His Leu Gly Ser Phe Ala Val Ser Ser Phe Phe 

                85                  90                  95      

Pro Leu Met Ala Gln Arg Arg Trp Lys Pro Asp Arg Ile Ile Gly Val 

            100                 105                 110         

Val Pro Thr Leu Phe Cys Ala Pro Gly Met Arg Leu Leu Ala Lys Leu 

        115                 120                 125             

Ser Gly Ala Arg Thr Val Leu His Ile Gln Asp Tyr Glu Val Asp Ala 

    130                 135                 140                 

Met Leu Gly Leu Gly Leu Ala Gly Lys Gly Lys Gly Gly Lys Val Ala 

145                 150                 155                 160 

Gln Leu Ala Thr Ala Phe Glu Arg Ser Gly Leu His Asn Val Asp Asn 

                165                 170                 175     

Val Ser Thr Ile Ser Arg Ser Met Met Asn Lys Ala Ile Glu Lys Gly 

            180                 185                 190         

Val Ala Ala Glu Asn Val Ile Phe Phe Pro Asn Trp Ser Glu Ile Ala 

        195                 200                 205             

Arg Phe Gln His Val Ala Asp Ala Asp Val Asp Ala Leu Arg Asn Gln 

    210                 215                 220                 

Leu Asp Leu Pro Asp Asn Lys Lys Ile Ile Leu Tyr Ser Gly Asn Ile 

225                 230                 235                 240 

Gly Glu Lys Gln Gly Leu Glu Asn Val Ile Glu Ala Ala Asp Arg Leu 

                245                 250                 255     

Arg Asp Glu Pro Leu Ile Phe Ala Ile Val Gly Gln Gly Gly Gly Lys 

            260                 265                 270         

Ala Arg Leu Glu Lys Met Ala Gln Gln Arg Gly Leu Arg Asn Met Gln 

        275                 280                 285             

Phe Phe Pro Leu Gln Ser Tyr Asp Ala Leu Pro Ala Leu Leu Lys Met 

    290                 295                 300                 

Gly Asp Cys His Leu Val Val Gln Lys Arg Gly Ala Ala Asp Ala Val 

305                 310                 315                 320 

Leu Pro Ser Lys Leu Thr Asn Ile Leu Ala Val Gly Gly Asn Ala Val 

                325                 330                 335     

Ile Thr Ala Glu Ala Tyr Thr Glu Leu Gly Gln Leu Cys Glu Thr Phe 

            340                 345                 350         

Pro Gly Ile Ala Val Cys Val Glu Pro Glu Ser Val Glu Ala Leu Val 

        355                 360                 365             

Ala Gly Ile Arg Gln Ala Leu Leu Leu Pro Lys His Asn Thr Val Ala 

    370                 375                 380                 

Arg Glu Tyr Ala Glu Arg Thr Leu Asp Lys Glu Asn Val Leu Arg Gln 

385                 390                 395                 400 

Phe Ile Asn Asp Ile Arg Gly 

                405         



<210> 38

<211> 407

<212> PRT

<213> Escherichia





<220> 

<223> WcaI, colanic acid biosynthesis fucosyltransferase , GenBank ID:

      WP_000699693.1



<400> 38

Met Lys Ile Leu Val Tyr Gly Ile Asn Tyr Ser Pro Glu Leu Thr Gly 

1               5                   10                  15      

Ile Gly Lys Tyr Thr Gly Glu Met Val Glu Trp Leu Ala Ala Gln Gly 

            20                  25                  30          

His Glu Val Arg Val Ile Thr Ala Pro Pro Tyr Tyr Pro Gln Trp Gln 

        35                  40                  45              

Val Gly Glu Asn Tyr Ser Ala Trp Arg Tyr Lys Arg Glu Glu Gly Ala 

    50                  55                  60                  

Ala Thr Val Trp Arg Cys Pro Leu Tyr Val Pro Lys Gln Pro Ser Thr 

65                  70                  75                  80  

Leu Lys Arg Leu Leu His Leu Gly Ser Phe Ala Val Ser Ser Phe Phe 

                85                  90                  95      

Pro Leu Met Ala Gln Arg Arg Trp Lys Pro Asp Arg Ile Ile Gly Val 

            100                 105                 110         

Val Pro Thr Leu Phe Cys Ala Pro Gly Met Arg Leu Leu Ala Lys Leu 

        115                 120                 125             

Ser Gly Ala Arg Thr Val Leu His Ile Gln Asp Tyr Glu Val Asp Ala 

    130                 135                 140                 

Met Leu Gly Leu Gly Leu Ala Gly Lys Gly Lys Gly Gly Lys Val Ala 

145                 150                 155                 160 

Gln Leu Ala Thr Ala Phe Glu Arg Ser Gly Leu His Asn Val Asp Asn 

                165                 170                 175     

Val Ser Thr Ile Ser Arg Ser Met Met Asn Lys Ala Ile Glu Lys Gly 

            180                 185                 190         

Val Ala Ala Glu Asn Val Ile Phe Phe Pro Asn Trp Ser Glu Ile Ala 

        195                 200                 205             

Arg Phe Gln His Val Ala Asp Ala Asp Val Asp Ala Leu Arg Asn Gln 

    210                 215                 220                 

Leu Asp Leu Pro Asp Asn Lys Lys Ile Ile Leu Tyr Ser Gly Asn Ile 

225                 230                 235                 240 

Gly Glu Lys Gln Gly Leu Glu Asn Val Ile Glu Ala Ala Asp Arg Leu 

                245                 250                 255     

Arg Asp Glu Pro Leu Ile Phe Ala Ile Val Gly Gln Gly Gly Gly Lys 

            260                 265                 270         

Ala Arg Leu Glu Lys Met Ala Gln Gln Arg Gly Leu Arg Asn Met Gln 

        275                 280                 285             

Phe Phe Pro Leu Gln Ser Tyr Asp Ala Leu Pro Ala Leu Leu Lys Met 

    290                 295                 300                 

Gly Asp Cys His Leu Val Val Gln Lys Arg Gly Ala Ala Asp Ala Val 

305                 310                 315                 320 

Leu Pro Ser Lys Leu Thr Asn Ile Leu Ala Val Gly Gly Asn Ala Val 

                325                 330                 335     

Ile Thr Ala Glu Ala Tyr Thr Glu Leu Gly Gln Leu Cys Glu Thr Phe 

            340                 345                 350         

Pro Gly Ile Ala Val Cys Val Glu Pro Glu Ser Val Glu Ala Leu Val 

        355                 360                 365             

Ala Gly Ile Arg Gln Ala Leu Leu Leu Pro Lys His Asn Thr Val Ala 

    370                 375                 380                 

Arg Glu Tyr Ala Glu Arg Thr Leu Asp Lys Glu Asn Val Leu Arg Gln 

385                 390                 395                 400 

Phe Ile Asn Asp Ile Arg Gly 

                405         



<210> 39

<211> 478

<212> PRT

<213> Escherichia coli K-12





<220> 

<223> ManC, mannose-1-phosphate guanylyltransferase, GenBank ID:

      NP_416553.1



<400> 39

Met Ala Gln Ser Lys Leu Tyr Pro Val Val Met Ala Gly Gly Ser Gly 

1               5                   10                  15      

Ser Arg Leu Trp Pro Leu Ser Arg Val Leu Tyr Pro Lys Gln Phe Leu 

            20                  25                  30          

Cys Leu Lys Gly Asp Leu Thr Met Leu Gln Thr Thr Ile Cys Arg Leu 

        35                  40                  45              

Asn Gly Val Glu Cys Glu Ser Pro Val Val Ile Cys Asn Glu Gln His 

    50                  55                  60                  

Arg Phe Ile Val Ala Glu Gln Leu Arg Gln Leu Asn Lys Leu Thr Glu 

65                  70                  75                  80  

Asn Ile Ile Leu Glu Pro Ala Gly Arg Asn Thr Ala Pro Ala Ile Ala 

                85                  90                  95      

Leu Ala Ala Leu Ala Ala Lys Arg His Ser Pro Glu Ser Asp Pro Leu 

            100                 105                 110         

Met Leu Val Leu Ala Ala Asp His Val Ile Ala Asp Glu Asp Ala Phe 

        115                 120                 125             

Arg Ala Ala Val Arg Asn Ala Met Pro Tyr Ala Glu Ala Gly Lys Leu 

    130                 135                 140                 

Val Thr Phe Gly Ile Val Pro Asp Leu Pro Glu Thr Gly Tyr Gly Tyr 

145                 150                 155                 160 

Ile Arg Arg Gly Glu Val Ser Ala Gly Glu Gln Asp Met Val Ala Phe 

                165                 170                 175     

Glu Val Ala Gln Phe Val Glu Lys Pro Asn Leu Glu Thr Ala Gln Ala 

            180                 185                 190         

Tyr Val Ala Ser Gly Glu Tyr Tyr Trp Asn Ser Gly Met Phe Leu Phe 

        195                 200                 205             

Arg Ala Gly Arg Tyr Leu Glu Glu Leu Lys Lys Tyr Arg Pro Asp Ile 

    210                 215                 220                 

Leu Asp Ala Cys Glu Lys Ala Met Ser Ala Val Asp Pro Asp Leu Asn 

225                 230                 235                 240 

Phe Ile Arg Val Asp Glu Glu Ala Phe Leu Ala Cys Pro Glu Glu Ser 

                245                 250                 255     

Val Asp Tyr Ala Val Met Glu Arg Thr Ala Asp Ala Val Val Val Pro 

            260                 265                 270         

Met Asp Ala Gly Trp Ser Asp Val Gly Ser Trp Ser Ser Leu Trp Glu 

        275                 280                 285             

Ile Ser Ala His Thr Ala Glu Gly Asn Val Cys His Gly Asp Val Ile 

    290                 295                 300                 

Asn His Lys Thr Glu Asn Ser Tyr Val Tyr Ala Glu Ser Gly Leu Val 

305                 310                 315                 320 

Thr Thr Val Gly Val Lys Asp Leu Val Val Val Gln Thr Lys Asp Ala 

                325                 330                 335     

Val Leu Ile Ala Asp Arg Asn Ala Val Gln Asp Val Lys Lys Val Val 

            340                 345                 350         

Glu Gln Ile Lys Ala Asp Gly Arg His Glu His Arg Val His Arg Glu 

        355                 360                 365             

Val Tyr Arg Pro Trp Gly Lys Tyr Asp Ser Ile Asp Ala Gly Asp Arg 

    370                 375                 380                 

Tyr Gln Val Lys Arg Ile Thr Val Lys Pro Gly Glu Gly Leu Ser Val 

385                 390                 395                 400 

Gln Met His His His Arg Ala Glu His Trp Val Val Val Ala Gly Thr 

                405                 410                 415     

Ala Lys Val Thr Ile Asp Gly Asp Ile Lys Leu Leu Gly Glu Asn Glu 

            420                 425                 430         

Ser Ile Tyr Ile Pro Leu Gly Ala Thr His Cys Leu Glu Asn Pro Gly 

        435                 440                 445             

Lys Ile Pro Leu Asp Leu Ile Glu Val Arg Ser Gly Ser Tyr Leu Glu 

    450                 455                 460                 

Glu Asp Asp Val Val Arg Phe Ala Asp Arg Tyr Gly Arg Val 

465                 470                 475             



<210> 40

<211> 478

<212> PRT

<213> Escherichia





<220> 

<223> ManC, mannose-1-phosphate guanyltransferase, GenBank ID:

      WP_000079274.1



<400> 40

Met Ala Gln Ser Lys Leu Tyr Pro Val Val Met Ala Gly Gly Ser Gly 

1               5                   10                  15      

Ser Arg Leu Trp Pro Leu Ser Arg Val Leu Tyr Pro Lys Gln Phe Leu 

            20                  25                  30          

Cys Leu Lys Gly Asp Leu Thr Met Leu Gln Thr Thr Ile Cys Arg Leu 

        35                  40                  45              

Asn Gly Val Glu Cys Glu Ser Pro Val Val Ile Cys Asn Glu Gln His 

    50                  55                  60                  

Arg Phe Ile Val Ala Glu Gln Leu Arg Gln Leu Asn Lys Leu Thr Glu 

65                  70                  75                  80  

Asn Ile Ile Leu Glu Pro Ala Gly Arg Asn Thr Ala Pro Ala Ile Ala 

                85                  90                  95      

Leu Ala Ala Leu Ala Ala Lys Arg His Ser Pro Glu Ser Asp Pro Leu 

            100                 105                 110         

Met Leu Val Leu Ala Ala Asp His Val Ile Ala Asp Glu Asp Ala Phe 

        115                 120                 125             

Arg Ala Ala Val Arg Asn Ala Met Pro Tyr Ala Glu Ala Gly Lys Leu 

    130                 135                 140                 

Val Thr Phe Gly Ile Val Pro Asp Leu Pro Glu Thr Gly Tyr Gly Tyr 

145                 150                 155                 160 

Ile Arg Arg Gly Glu Val Ser Ala Gly Glu Gln Asp Met Val Ala Phe 

                165                 170                 175     

Glu Val Ala Gln Phe Val Glu Lys Pro Asn Leu Glu Thr Ala Gln Ala 

            180                 185                 190         

Tyr Val Ala Ser Gly Glu Tyr Tyr Trp Asn Ser Gly Met Phe Leu Phe 

        195                 200                 205             

Arg Ala Gly Arg Tyr Leu Glu Glu Leu Lys Lys Tyr Arg Pro Asp Ile 

    210                 215                 220                 

Leu Asp Ala Cys Glu Lys Ala Met Ser Ala Val Asp Pro Asp Leu Asn 

225                 230                 235                 240 

Phe Ile Arg Val Asp Glu Glu Ala Phe Leu Ala Cys Pro Glu Glu Ser 

                245                 250                 255     

Val Asp Tyr Ala Val Met Glu Arg Thr Ala Asp Ala Val Val Val Pro 

            260                 265                 270         

Met Asp Ala Gly Trp Ser Asp Val Gly Ser Trp Ser Ser Leu Trp Glu 

        275                 280                 285             

Ile Ser Ala His Thr Ala Glu Gly Asn Val Cys His Gly Asp Val Ile 

    290                 295                 300                 

Asn His Lys Thr Glu Asn Ser Tyr Val Tyr Ala Glu Ser Gly Leu Val 

305                 310                 315                 320 

Thr Thr Val Gly Val Lys Asp Leu Val Val Val Gln Thr Lys Asp Ala 

                325                 330                 335     

Val Leu Ile Ala Asp Arg Asn Ala Val Gln Asp Val Lys Lys Val Val 

            340                 345                 350         

Glu Gln Ile Lys Ala Asp Gly Arg His Glu His Arg Val His Arg Glu 

        355                 360                 365             

Val Tyr Arg Pro Trp Gly Lys Tyr Asp Ser Ile Asp Ala Gly Asp Arg 

    370                 375                 380                 

Tyr Gln Val Lys Arg Ile Thr Val Lys Pro Gly Glu Gly Leu Ser Val 

385                 390                 395                 400 

Gln Met His His His Arg Ala Glu His Trp Val Val Val Ala Gly Thr 

                405                 410                 415     

Ala Lys Val Thr Ile Asp Gly Asp Ile Lys Leu Leu Gly Glu Asn Glu 

            420                 425                 430         

Ser Ile Tyr Ile Pro Leu Gly Ala Thr His Cys Leu Glu Asn Pro Gly 

        435                 440                 445             

Lys Ile Pro Leu Asp Leu Ile Glu Val Arg Ser Gly Ser Tyr Leu Glu 

    450                 455                 460                 

Glu Asp Asp Val Val Arg Phe Ala Asp Arg Tyr Gly Arg Val 

465                 470                 475             



<210> 41

<211> 456

<212> PRT

<213> Escherichia coli K-12





<220> 

<223> ManB, phosphomannomutase, GenBank ID: NP_416552.1



<400> 41

Met Lys Lys Leu Thr Cys Phe Lys Ala Tyr Asp Ile Arg Gly Lys Leu 

1               5                   10                  15      

Gly Glu Glu Leu Asn Glu Asp Ile Ala Trp Arg Ile Gly Arg Ala Tyr 

            20                  25                  30          

Gly Glu Phe Leu Lys Pro Lys Thr Ile Val Leu Gly Gly Asp Val Arg 

        35                  40                  45              

Leu Thr Ser Glu Thr Leu Lys Leu Ala Leu Ala Lys Gly Leu Gln Asp 

    50                  55                  60                  

Ala Gly Val Asp Val Leu Asp Ile Gly Met Ser Gly Thr Glu Glu Ile 

65                  70                  75                  80  

Tyr Phe Ala Thr Phe His Leu Gly Val Asp Gly Gly Ile Glu Val Thr 

                85                  90                  95      

Ala Ser His Asn Pro Met Asp Tyr Asn Gly Met Lys Leu Val Arg Glu 

            100                 105                 110         

Gly Ala Arg Pro Ile Ser Gly Asp Thr Gly Leu Arg Asp Val Gln Arg 

        115                 120                 125             

Leu Ala Glu Ala Asn Asp Phe Pro Pro Val Asp Glu Thr Lys Arg Gly 

    130                 135                 140                 

Arg Tyr Gln Gln Ile Asn Leu Arg Asp Ala Tyr Val Asp His Leu Phe 

145                 150                 155                 160 

Gly Tyr Ile Asn Val Lys Asn Leu Thr Pro Leu Lys Leu Val Ile Asn 

                165                 170                 175     

Ser Gly Asn Gly Ala Ala Gly Pro Val Val Asp Ala Ile Glu Ala Arg 

            180                 185                 190         

Phe Lys Ala Leu Gly Ala Pro Val Glu Leu Ile Lys Val His Asn Thr 

        195                 200                 205             

Pro Asp Gly Asn Phe Pro Asn Gly Ile Pro Asn Pro Leu Leu Pro Glu 

    210                 215                 220                 

Cys Arg Asp Asp Thr Arg Asn Ala Val Ile Lys His Gly Ala Asp Met 

225                 230                 235                 240 

Gly Ile Ala Phe Asp Gly Asp Phe Asp Arg Cys Phe Leu Phe Asp Glu 

                245                 250                 255     

Lys Gly Gln Phe Ile Glu Gly Tyr Tyr Ile Val Gly Leu Leu Ala Glu 

            260                 265                 270         

Ala Phe Leu Glu Lys Asn Pro Gly Ala Lys Ile Ile His Asp Pro Arg 

        275                 280                 285             

Leu Ser Trp Asn Thr Val Asp Val Val Thr Ala Ala Gly Gly Thr Pro 

    290                 295                 300                 

Val Met Ser Lys Thr Gly His Ala Phe Ile Lys Glu Arg Met Arg Lys 

305                 310                 315                 320 

Glu Asp Ala Ile Tyr Gly Gly Glu Met Ser Ala His His Tyr Phe Arg 

                325                 330                 335     

Asp Phe Ala Tyr Cys Asp Ser Gly Met Ile Pro Trp Leu Leu Val Ala 

            340                 345                 350         

Glu Leu Val Cys Leu Lys Asp Lys Thr Leu Gly Glu Leu Val Arg Asp 

        355                 360                 365             

Arg Met Ala Ala Phe Pro Ala Ser Gly Glu Ile Asn Ser Lys Leu Ala 

    370                 375                 380                 

Gln Pro Val Glu Ala Ile Asn Arg Val Glu Gln His Phe Ser Arg Glu 

385                 390                 395                 400 

Ala Leu Ala Val Asp Arg Thr Asp Gly Ile Ser Met Thr Phe Ala Asp 

                405                 410                 415     

Trp Arg Phe Asn Leu Arg Thr Ser Asn Thr Glu Pro Val Val Arg Leu 

            420                 425                 430         

Asn Val Glu Ser Arg Gly Asp Val Pro Leu Met Glu Ala Arg Thr Arg 

        435                 440                 445             

Thr Leu Leu Thr Leu Leu Asn Glu 

    450                 455     



<210> 42

<211> 456

<212> PRT

<213> Escherichia





<220> 

<223> ManB, phosphomannomutase, GenBank ID: WP_001350528.1



<400> 42

Met Lys Lys Leu Thr Cys Phe Lys Ala Tyr Asp Ile Arg Gly Lys Leu 

1               5                   10                  15      

Gly Glu Glu Leu Asn Glu Asp Ile Ala Trp Arg Ile Gly Arg Ala Tyr 

            20                  25                  30          

Gly Glu Phe Leu Lys Pro Lys Thr Ile Val Leu Gly Gly Asp Val Arg 

        35                  40                  45              

Leu Thr Ser Glu Thr Leu Lys Leu Ala Leu Ala Lys Gly Leu Gln Asp 

    50                  55                  60                  

Ala Gly Val Asp Val Leu Asp Ile Gly Met Ser Gly Thr Glu Glu Ile 

65                  70                  75                  80  

Tyr Phe Ala Thr Phe His Leu Gly Val Asp Gly Gly Ile Glu Val Thr 

                85                  90                  95      

Ala Ser His Asn Pro Met Asp Tyr Asn Gly Met Lys Leu Val Arg Glu 

            100                 105                 110         

Gly Ala Arg Pro Ile Ser Gly Asp Thr Gly Leu Arg Asp Val Gln Arg 

        115                 120                 125             

Leu Ala Glu Ala Asn Asp Phe Pro Pro Val Asp Glu Thr Lys Arg Gly 

    130                 135                 140                 

Arg Tyr Gln Gln Ile Asn Leu Arg Asp Ala Tyr Val Asp His Leu Phe 

145                 150                 155                 160 

Gly Tyr Ile Asn Val Lys Asn Leu Thr Pro Leu Lys Leu Val Ile Asn 

                165                 170                 175     

Ser Gly Asn Gly Ala Ala Gly Pro Val Val Asp Ala Ile Glu Ala Arg 

            180                 185                 190         

Phe Lys Ala Leu Gly Ala Pro Val Glu Leu Ile Lys Val His Asn Thr 

        195                 200                 205             

Pro Asp Gly Asn Phe Pro Asn Gly Ile Pro Asn Pro Leu Leu Pro Glu 

    210                 215                 220                 

Cys Arg Asp Asp Thr Arg Asn Ala Val Ile Lys His Gly Ala Asp Met 

225                 230                 235                 240 

Gly Ile Ala Phe Asp Gly Asp Phe Asp Arg Cys Phe Leu Phe Asp Glu 

                245                 250                 255     

Lys Gly Gln Phe Ile Glu Gly Tyr Tyr Ile Val Gly Leu Leu Ala Glu 

            260                 265                 270         

Ala Phe Leu Glu Lys Asn Pro Gly Ala Lys Ile Ile His Asp Pro Arg 

        275                 280                 285             

Leu Ser Trp Asn Thr Val Asp Val Val Thr Ala Ala Gly Gly Thr Pro 

    290                 295                 300                 

Val Met Ser Lys Thr Gly His Ala Phe Ile Lys Glu Arg Met Arg Lys 

305                 310                 315                 320 

Glu Asp Ala Ile Tyr Gly Gly Glu Met Ser Ala His His Tyr Phe Arg 

                325                 330                 335     

Asp Phe Ala Tyr Cys Asp Ser Gly Met Ile Pro Trp Leu Leu Val Ala 

            340                 345                 350         

Glu Leu Val Cys Leu Lys Asp Lys Thr Leu Gly Glu Leu Val Arg Asp 

        355                 360                 365             

Arg Met Ala Ala Phe Pro Ala Ser Gly Glu Ile Asn Ser Lys Leu Ala 

    370                 375                 380                 

Gln Pro Val Glu Ala Ile Asn Arg Val Glu Gln His Phe Ser Arg Glu 

385                 390                 395                 400 

Ala Leu Ala Val Asp Arg Thr Asp Gly Ile Ser Met Thr Phe Ala Asp 

                405                 410                 415     

Trp Arg Phe Asn Leu Arg Thr Ser Asn Thr Glu Pro Val Val Arg Leu 

            420                 425                 430         

Asn Val Glu Ser Arg Gly Asp Val Pro Leu Met Glu Ala Arg Thr Arg 

        435                 440                 445             

Thr Leu Leu Thr Leu Leu Asn Glu 

    450                 455     



<210> 43

<211> 546

<212> PRT

<213> Escherichia coli K-12





<220> 

<223> pgm, Phosphoglucomutase, GenBank ID: NP_415214.1



<400> 43

Met Ala Ile His Asn Arg Ala Gly Gln Pro Ala Gln Gln Ser Asp Leu 

1               5                   10                  15      

Ile Asn Val Ala Gln Leu Thr Ala Gln Tyr Tyr Val Leu Lys Pro Glu 

            20                  25                  30          

Ala Gly Asn Ala Glu His Ala Val Lys Phe Gly Thr Ser Gly His Arg 

        35                  40                  45              

Gly Ser Ala Ala Arg His Ser Phe Asn Glu Pro His Ile Leu Ala Ile 

    50                  55                  60                  

Ala Gln Ala Ile Ala Glu Glu Arg Ala Lys Asn Gly Ile Thr Gly Pro 

65                  70                  75                  80  

Cys Tyr Val Gly Lys Asp Thr His Ala Leu Ser Glu Pro Ala Phe Ile 

                85                  90                  95      

Ser Val Leu Glu Val Leu Ala Ala Asn Gly Val Asp Val Ile Val Gln 

            100                 105                 110         

Glu Asn Asn Gly Phe Thr Pro Thr Pro Ala Val Ser Asn Ala Ile Leu 

        115                 120                 125             

Val His Asn Lys Lys Gly Gly Pro Leu Ala Asp Gly Ile Val Ile Thr 

    130                 135                 140                 

Pro Ser His Asn Pro Pro Glu Asp Gly Gly Ile Lys Tyr Asn Pro Pro 

145                 150                 155                 160 

Asn Gly Gly Pro Ala Asp Thr Asn Val Thr Lys Val Val Glu Asp Arg 

                165                 170                 175     

Ala Asn Ala Leu Leu Ala Asp Gly Leu Lys Gly Val Lys Arg Ile Ser 

            180                 185                 190         

Leu Asp Glu Ala Met Ala Ser Gly His Val Lys Glu Gln Asp Leu Val 

        195                 200                 205             

Gln Pro Phe Val Glu Gly Leu Ala Asp Ile Val Asp Met Ala Ala Ile 

    210                 215                 220                 

Gln Lys Ala Gly Leu Thr Leu Gly Val Asp Pro Leu Gly Gly Ser Gly 

225                 230                 235                 240 

Ile Glu Tyr Trp Lys Arg Ile Gly Glu Tyr Tyr Asn Leu Asn Leu Thr 

                245                 250                 255     

Ile Val Asn Asp Gln Val Asp Gln Thr Phe Arg Phe Met His Leu Asp 

            260                 265                 270         

Lys Asp Gly Ala Ile Arg Met Asp Cys Ser Ser Glu Cys Ala Met Ala 

        275                 280                 285             

Gly Leu Leu Ala Leu Arg Asp Lys Phe Asp Leu Ala Phe Ala Asn Asp 

    290                 295                 300                 

Pro Asp Tyr Asp Arg His Gly Ile Val Thr Pro Ala Gly Leu Met Asn 

305                 310                 315                 320 

Pro Asn His Tyr Leu Ala Val Ala Ile Asn Tyr Leu Phe Gln His Arg 

                325                 330                 335     

Pro Gln Trp Gly Lys Asp Val Ala Val Gly Lys Thr Leu Val Ser Ser 

            340                 345                 350         

Ala Met Ile Asp Arg Val Val Asn Asp Leu Gly Arg Lys Leu Val Glu 

        355                 360                 365             

Val Pro Val Gly Phe Lys Trp Phe Val Asp Gly Leu Phe Asp Gly Ser 

    370                 375                 380                 

Phe Gly Phe Gly Gly Glu Glu Ser Ala Gly Ala Ser Phe Leu Arg Phe 

385                 390                 395                 400 

Asp Gly Thr Pro Trp Ser Thr Asp Lys Asp Gly Ile Ile Met Cys Leu 

                405                 410                 415     

Leu Ala Ala Glu Ile Thr Ala Val Thr Gly Lys Asn Pro Gln Glu His 

            420                 425                 430         

Tyr Asn Glu Leu Ala Lys Arg Phe Gly Ala Pro Ser Tyr Asn Arg Leu 

        435                 440                 445             

Gln Ala Ala Ala Thr Ser Ala Gln Lys Ala Ala Leu Ser Lys Leu Ser 

    450                 455                 460                 

Pro Glu Met Val Ser Ala Ser Thr Leu Ala Gly Asp Pro Ile Thr Ala 

465                 470                 475                 480 

Arg Leu Thr Ala Ala Pro Gly Asn Gly Ala Ser Ile Gly Gly Leu Lys 

                485                 490                 495     

Val Met Thr Asp Asn Gly Trp Phe Ala Ala Arg Pro Ser Gly Thr Glu 

            500                 505                 510         

Asp Ala Tyr Lys Ile Tyr Cys Glu Ser Phe Leu Gly Glu Glu His Arg 

        515                 520                 525             

Lys Gln Ile Glu Lys Glu Ala Val Glu Ile Val Ser Glu Val Leu Lys 

    530                 535                 540                 

Asn Ala 

545     



<210> 44

<211> 553

<212> PRT

<213> Escherichia coli





<220> 

<223> pgm, phosphoglucomutase, GenBank ID: WP_001396326.1



<400> 44

Met Gln Thr Lys Asp Lys Ala Met Ala Ile His Asn Arg Ala Gly Gln 

1               5                   10                  15      

Pro Ala Gln Gln Ser Asp Leu Ile Asn Val Ala Gln Leu Thr Ala Gln 

            20                  25                  30          

Tyr Tyr Val Leu Lys Pro Glu Ala Gly Asn Ala Glu His Ala Val Lys 

        35                  40                  45              

Phe Gly Thr Ser Gly His Arg Gly Ser Ala Ala Arg His Ser Phe Asn 

    50                  55                  60                  

Glu Pro His Ile Leu Ala Ile Ala Gln Ala Ile Ala Glu Glu Arg Ala 

65                  70                  75                  80  

Lys Asn Gly Ile Thr Gly Pro Cys Tyr Val Gly Lys Asp Thr His Ala 

                85                  90                  95      

Leu Ser Glu Pro Ala Phe Ile Ser Val Leu Glu Val Leu Ala Ala Asn 

            100                 105                 110         

Gly Val Asp Val Ile Val Gln Glu Asn Asn Gly Phe Thr Pro Thr Pro 

        115                 120                 125             

Ala Val Ser Asn Ala Ile Leu Val His Asn Lys Lys Gly Gly Pro Leu 

    130                 135                 140                 

Ala Asp Gly Ile Val Ile Thr Pro Ser His Asn Pro Pro Glu Asp Gly 

145                 150                 155                 160 

Gly Ile Lys Tyr Asn Pro Pro Asn Gly Gly Pro Ala Asp Thr Asn Val 

                165                 170                 175     

Thr Lys Val Val Glu Asp Arg Ala Asn Ala Leu Leu Ala Asp Gly Leu 

            180                 185                 190         

Lys Gly Val Lys Arg Ile Ser Leu Asp Glu Ala Met Ala Ser Gly His 

        195                 200                 205             

Val Lys Glu Gln Asp Leu Val Gln Pro Phe Val Glu Gly Leu Ala Asp 

    210                 215                 220                 

Ile Val Asp Met Ala Ala Ile Gln Lys Ala Gly Leu Thr Leu Gly Val 

225                 230                 235                 240 

Asp Pro Leu Gly Gly Ser Gly Ile Glu Tyr Trp Lys Arg Ile Gly Glu 

                245                 250                 255     

Tyr Tyr Asn Leu Asn Leu Thr Ile Val Asn Asp Gln Val Asp Gln Thr 

            260                 265                 270         

Phe Arg Phe Met His Leu Asp Lys Asp Gly Ala Ile Arg Met Asp Cys 

        275                 280                 285             

Ser Ser Glu Cys Ala Met Ala Gly Leu Leu Ala Leu Arg Asp Lys Phe 

    290                 295                 300                 

Asp Leu Ala Phe Ala Asn Asp Pro Asp Tyr Asp Arg His Gly Ile Val 

305                 310                 315                 320 

Thr Pro Ala Gly Leu Met Asn Pro Asn His Tyr Leu Ala Val Ala Ile 

                325                 330                 335     

Asn Tyr Leu Phe Gln His Arg Pro Gln Trp Gly Lys Asp Val Ala Val 

            340                 345                 350         

Gly Lys Thr Leu Val Ser Ser Ala Met Ile Asp Arg Val Val Asn Asp 

        355                 360                 365             

Leu Gly Arg Lys Leu Val Glu Val Pro Val Gly Phe Lys Trp Phe Val 

    370                 375                 380                 

Asp Gly Leu Phe Asp Gly Ser Phe Gly Phe Gly Gly Glu Glu Ser Ala 

385                 390                 395                 400 

Gly Ala Ser Phe Leu Arg Phe Asp Gly Thr Pro Trp Ser Thr Asp Lys 

                405                 410                 415     

Asp Gly Ile Ile Met Cys Leu Leu Ala Ala Glu Ile Thr Ala Val Thr 

            420                 425                 430         

Gly Lys Asn Pro Gln Glu His Tyr Asn Glu Leu Ala Lys Arg Phe Gly 

        435                 440                 445             

Ala Pro Ser Tyr Asn Arg Leu Gln Ala Ala Ala Thr Ser Ala Gln Lys 

    450                 455                 460                 

Ala Ala Leu Ser Lys Leu Ser Pro Glu Met Val Ser Ala Ser Thr Leu 

465                 470                 475                 480 

Ala Gly Asp Pro Ile Thr Ala Arg Leu Thr Ala Ala Pro Gly Asn Gly 

                485                 490                 495     

Ala Ser Ile Gly Gly Leu Lys Val Met Thr Asp Asn Gly Trp Phe Ala 

            500                 505                 510         

Ala Arg Pro Ser Gly Thr Glu Asp Ala Tyr Lys Ile Tyr Cys Glu Ser 

        515                 520                 525             

Phe Leu Gly Glu Glu His Arg Lys Gln Ile Glu Lys Glu Ala Val Glu 

    530                 535                 540                 

Ile Val Ser Glu Val Leu Lys Asn Ala 

545                 550             



<210> 45

<211> 302

<212> PRT

<213> Escherichia coli K-12





<220> 

<223> GalU, UTP-glucose-1-phosphate uridylyltransferase, GenBank:

      NP_415752.1



<400> 45

Met Ala Ala Ile Asn Thr Lys Val Lys Lys Ala Val Ile Pro Val Ala 

1               5                   10                  15      

Gly Leu Gly Thr Arg Met Leu Pro Ala Thr Lys Ala Ile Pro Lys Glu 

            20                  25                  30          

Met Leu Pro Leu Val Asp Lys Pro Leu Ile Gln Tyr Val Val Asn Glu 

        35                  40                  45              

Cys Ile Ala Ala Gly Ile Thr Glu Ile Val Leu Val Thr His Ser Ser 

    50                  55                  60                  

Lys Asn Ser Ile Glu Asn His Phe Asp Thr Ser Phe Glu Leu Glu Ala 

65                  70                  75                  80  

Met Leu Glu Lys Arg Val Lys Arg Gln Leu Leu Asp Glu Val Gln Ser 

                85                  90                  95      

Ile Cys Pro Pro His Val Thr Ile Met Gln Val Arg Gln Gly Leu Ala 

            100                 105                 110         

Lys Gly Leu Gly His Ala Val Leu Cys Ala His Pro Val Val Gly Asp 

        115                 120                 125             

Glu Pro Val Ala Val Ile Leu Pro Asp Val Ile Leu Asp Glu Tyr Glu 

    130                 135                 140                 

Ser Asp Leu Ser Gln Asp Asn Leu Ala Glu Met Ile Arg Arg Phe Asp 

145                 150                 155                 160 

Glu Thr Gly His Ser Gln Ile Met Val Glu Pro Val Ala Asp Val Thr 

                165                 170                 175     

Ala Tyr Gly Val Val Asp Cys Lys Gly Val Glu Leu Ala Pro Gly Glu 

            180                 185                 190         

Ser Val Pro Met Val Gly Val Val Glu Lys Pro Lys Ala Asp Val Ala 

        195                 200                 205             

Pro Ser Asn Leu Ala Ile Val Gly Arg Tyr Val Leu Ser Ala Asp Ile 

    210                 215                 220                 

Trp Pro Leu Leu Ala Lys Thr Pro Pro Gly Ala Gly Asp Glu Ile Gln 

225                 230                 235                 240 

Leu Thr Asp Ala Ile Asp Met Leu Ile Glu Lys Glu Thr Val Glu Ala 

                245                 250                 255     

Tyr His Met Lys Gly Lys Ser His Asp Cys Gly Asn Lys Leu Gly Tyr 

            260                 265                 270         

Met Gln Ala Phe Val Glu Tyr Gly Ile Arg His Asn Thr Leu Gly Thr 

        275                 280                 285             

Glu Phe Lys Ala Trp Leu Glu Glu Glu Met Gly Ile Lys Lys 

    290                 295                 300         



<210> 46

<211> 302

<212> PRT

<213> Enterobacteriaceae





<220> 

<223> GalU, UTP-glucose-1-phosphate uridylyltransferase, WP_000718995.1



<400> 46

Met Ala Ala Ile Asn Thr Lys Val Lys Lys Ala Val Ile Pro Val Ala 

1               5                   10                  15      

Gly Leu Gly Thr Arg Met Leu Pro Ala Thr Lys Ala Ile Pro Lys Glu 

            20                  25                  30          

Met Leu Pro Leu Val Asp Lys Pro Leu Ile Gln Tyr Val Val Asn Glu 

        35                  40                  45              

Cys Ile Ala Ala Gly Ile Thr Glu Ile Val Leu Val Thr His Ser Ser 

    50                  55                  60                  

Lys Asn Ser Ile Glu Asn His Phe Asp Thr Ser Phe Glu Leu Glu Ala 

65                  70                  75                  80  

Met Leu Glu Lys Arg Val Lys Arg Gln Leu Leu Asp Glu Val Gln Ser 

                85                  90                  95      

Ile Cys Pro Pro His Val Thr Ile Met Gln Val Arg Gln Gly Leu Ala 

            100                 105                 110         

Lys Gly Leu Gly His Ala Val Leu Cys Ala His Pro Val Val Gly Asp 

        115                 120                 125             

Glu Pro Val Ala Val Ile Leu Pro Asp Val Ile Leu Asp Glu Tyr Glu 

    130                 135                 140                 

Ser Asp Leu Ser Gln Asp Asn Leu Ala Glu Met Ile Arg Arg Phe Asp 

145                 150                 155                 160 

Glu Thr Gly His Ser Gln Ile Met Val Glu Pro Val Ala Asp Val Thr 

                165                 170                 175     

Ala Tyr Gly Val Val Asp Cys Lys Gly Val Glu Leu Ala Pro Gly Glu 

            180                 185                 190         

Ser Val Pro Met Val Gly Val Val Glu Lys Pro Lys Ala Asp Val Ala 

        195                 200                 205             

Pro Ser Asn Leu Ala Ile Val Gly Arg Tyr Val Leu Ser Ala Asp Ile 

    210                 215                 220                 

Trp Pro Leu Leu Ala Lys Thr Pro Pro Gly Ala Gly Asp Glu Ile Gln 

225                 230                 235                 240 

Leu Thr Asp Ala Ile Asp Met Leu Ile Glu Lys Glu Thr Val Glu Ala 

                245                 250                 255     

Tyr His Met Lys Gly Lys Ser His Asp Cys Gly Asn Lys Leu Gly Tyr 

            260                 265                 270         

Met Gln Ala Phe Val Glu Tyr Gly Ile Arg His Asn Thr Leu Gly Thr 

        275                 280                 285             

Glu Phe Lys Ala Trp Leu Glu Glu Glu Met Gly Ile Lys Lys 

    290                 295                 300         



<210> 47

<211> 338

<212> PRT

<213> Escherichia coli K-12





<220> 

<223> GalE, UDP-glucose 4-epimerase, GenBank ID: NP_415280.3



<400> 47

Met Arg Val Leu Val Thr Gly Gly Ser Gly Tyr Ile Gly Ser His Thr 

1               5                   10                  15      

Cys Val Gln Leu Leu Gln Asn Gly His Asp Val Ile Ile Leu Asp Asn 

            20                  25                  30          

Leu Cys Asn Ser Lys Arg Ser Val Leu Pro Val Ile Glu Arg Leu Gly 

        35                  40                  45              

Gly Lys His Pro Thr Phe Val Glu Gly Asp Ile Arg Asn Glu Ala Leu 

    50                  55                  60                  

Met Thr Glu Ile Leu His Asp His Ala Ile Asp Thr Val Ile His Phe 

65                  70                  75                  80  

Ala Gly Leu Lys Ala Val Gly Glu Ser Val Gln Lys Pro Leu Glu Tyr 

                85                  90                  95      

Tyr Asp Asn Asn Val Asn Gly Thr Leu Arg Leu Ile Ser Ala Met Arg 

            100                 105                 110         

Ala Ala Asn Val Lys Asn Phe Ile Phe Ser Ser Ser Ala Thr Val Tyr 

        115                 120                 125             

Gly Asp Gln Pro Lys Ile Pro Tyr Val Glu Ser Phe Pro Thr Gly Thr 

    130                 135                 140                 

Pro Gln Ser Pro Tyr Gly Lys Ser Lys Leu Met Val Glu Gln Ile Leu 

145                 150                 155                 160 

Thr Asp Leu Gln Lys Ala Gln Pro Asp Trp Ser Ile Ala Leu Leu Arg 

                165                 170                 175     

Tyr Phe Asn Pro Val Gly Ala His Pro Ser Gly Asp Met Gly Glu Asp 

            180                 185                 190         

Pro Gln Gly Ile Pro Asn Asn Leu Met Pro Tyr Ile Ala Gln Val Ala 

        195                 200                 205             

Val Gly Arg Arg Asp Ser Leu Ala Ile Phe Gly Asn Asp Tyr Pro Thr 

    210                 215                 220                 

Glu Asp Gly Thr Gly Val Arg Asp Tyr Ile His Val Met Asp Leu Ala 

225                 230                 235                 240 

Asp Gly His Val Val Ala Met Glu Lys Leu Ala Asn Lys Pro Gly Val 

                245                 250                 255     

His Ile Tyr Asn Leu Gly Ala Gly Val Gly Asn Ser Val Leu Asp Val 

            260                 265                 270         

Val Asn Ala Phe Ser Lys Ala Cys Gly Lys Pro Val Asn Tyr His Phe 

        275                 280                 285             

Ala Pro Arg Arg Glu Gly Asp Leu Pro Ala Tyr Trp Ala Asp Ala Ser 

    290                 295                 300                 

Lys Ala Asp Arg Glu Leu Asn Trp Arg Val Thr Arg Thr Leu Asp Glu 

305                 310                 315                 320 

Met Ala Gln Asp Thr Trp His Trp Gln Ser Arg His Pro Gln Gly Tyr 

                325                 330                 335     

Pro Asp 

        



<210> 48

<211> 338

<212> PRT

<213> Enterobacteriaceae





<220> 

<223> GalE, UDP-glucose 4-epimerase, GenBank ID: WP_001265438.1



<400> 48

Met Arg Val Leu Val Thr Gly Gly Ser Gly Tyr Ile Gly Ser His Thr 

1               5                   10                  15      

Cys Val Gln Leu Leu Gln Asn Gly His Asp Val Ile Ile Leu Asp Asn 

            20                  25                  30          

Leu Cys Asn Ser Lys Arg Ser Val Leu Pro Val Ile Glu Arg Leu Gly 

        35                  40                  45              

Gly Lys His Pro Thr Phe Val Glu Gly Asp Ile Arg Asn Glu Ala Leu 

    50                  55                  60                  

Met Thr Glu Ile Leu His Asp His Ala Ile Asp Thr Val Ile His Phe 

65                  70                  75                  80  

Ala Gly Leu Lys Ala Val Gly Glu Ser Val Gln Lys Pro Leu Glu Tyr 

                85                  90                  95      

Tyr Asp Asn Asn Val Asn Gly Thr Leu Arg Leu Ile Ser Ala Met Arg 

            100                 105                 110         

Ala Ala Asn Val Lys Asn Phe Ile Phe Ser Ser Ser Ala Thr Val Tyr 

        115                 120                 125             

Gly Asp Gln Pro Lys Ile Pro Tyr Val Glu Ser Phe Pro Thr Gly Thr 

    130                 135                 140                 

Pro Gln Ser Pro Tyr Gly Lys Ser Lys Leu Met Val Glu Gln Ile Leu 

145                 150                 155                 160 

Thr Asp Leu Gln Lys Ala Gln Pro Asp Trp Ser Ile Ala Leu Leu Arg 

                165                 170                 175     

Tyr Phe Asn Pro Val Gly Ala His Pro Ser Gly Asp Met Gly Glu Asp 

            180                 185                 190         

Pro Gln Gly Ile Pro Asn Asn Leu Met Pro Tyr Ile Ala Gln Val Ala 

        195                 200                 205             

Val Gly Arg Arg Asp Ser Leu Ala Ile Phe Gly Asn Asp Tyr Pro Thr 

    210                 215                 220                 

Glu Asp Gly Thr Gly Val Arg Asp Tyr Ile His Val Met Asp Leu Ala 

225                 230                 235                 240 

Asp Gly His Val Val Ala Met Glu Lys Leu Ala Asn Lys Pro Gly Val 

                245                 250                 255     

His Ile Tyr Asn Leu Gly Ala Gly Val Gly Asn Ser Val Leu Asp Val 

            260                 265                 270         

Val Asn Ala Phe Ser Lys Ala Cys Gly Lys Pro Val Asn Tyr His Phe 

        275                 280                 285             

Ala Pro Arg Arg Glu Gly Asp Leu Pro Ala Tyr Trp Ala Asp Ala Ser 

    290                 295                 300                 

Lys Ala Asp Arg Glu Leu Asn Trp Arg Val Thr Arg Thr Leu Asp Glu 

305                 310                 315                 320 

Met Ala Gln Asp Thr Trp His Trp Gln Ser Arg His Pro Gln Gly Tyr 

                325                 330                 335     

Pro Asp 

        



<210> 49

<211> 445

<212> PRT

<213> Escherichia coli K-12





<220> 

<223> GlmM, Phosphoglucosamine mutase, GenBank ID: NP_417643.1



<400> 49

Met Ser Asn Arg Lys Tyr Phe Gly Thr Asp Gly Ile Arg Gly Arg Val 

1               5                   10                  15      

Gly Asp Ala Pro Ile Thr Pro Asp Phe Val Leu Lys Leu Gly Trp Ala 

            20                  25                  30          

Ala Gly Lys Val Leu Ala Arg His Gly Ser Arg Lys Ile Ile Ile Gly 

        35                  40                  45              

Lys Asp Thr Arg Ile Ser Gly Tyr Met Leu Glu Ser Ala Leu Glu Ala 

    50                  55                  60                  

Gly Leu Ala Ala Ala Gly Leu Ser Ala Leu Phe Thr Gly Pro Met Pro 

65                  70                  75                  80  

Thr Pro Ala Val Ala Tyr Leu Thr Arg Thr Phe Arg Ala Glu Ala Gly 

                85                  90                  95      

Ile Val Ile Ser Ala Ser His Asn Pro Phe Tyr Asp Asn Gly Ile Lys 

            100                 105                 110         

Phe Phe Ser Ile Asp Gly Thr Lys Leu Pro Asp Ala Val Glu Glu Ala 

        115                 120                 125             

Ile Glu Ala Glu Met Glu Lys Glu Ile Ser Cys Val Asp Ser Ala Glu 

    130                 135                 140                 

Leu Gly Lys Ala Ser Arg Ile Val Asp Ala Ala Gly Arg Tyr Ile Glu 

145                 150                 155                 160 

Phe Cys Lys Ala Thr Phe Pro Asn Glu Leu Ser Leu Ser Glu Leu Lys 

                165                 170                 175     

Ile Val Val Asp Cys Ala Asn Gly Ala Thr Tyr His Ile Ala Pro Asn 

            180                 185                 190         

Val Leu Arg Glu Leu Gly Ala Asn Val Ile Ala Ile Gly Cys Glu Pro 

        195                 200                 205             

Asn Gly Val Asn Ile Asn Ala Glu Val Gly Ala Thr Asp Val Arg Ala 

    210                 215                 220                 

Leu Gln Ala Arg Val Leu Ala Glu Lys Ala Asp Leu Gly Ile Ala Phe 

225                 230                 235                 240 

Asp Gly Asp Gly Asp Arg Val Ile Met Val Asp His Glu Gly Asn Lys 

                245                 250                 255     

Val Asp Gly Asp Gln Ile Met Tyr Ile Ile Ala Arg Glu Gly Leu Arg 

            260                 265                 270         

Gln Gly Gln Leu Arg Gly Gly Ala Val Gly Thr Leu Met Ser Asn Met 

        275                 280                 285             

Gly Leu Glu Leu Ala Leu Lys Gln Leu Gly Ile Pro Phe Ala Arg Ala 

    290                 295                 300                 

Lys Val Gly Asp Arg Tyr Val Leu Glu Lys Met Gln Glu Lys Gly Trp 

305                 310                 315                 320 

Arg Ile Gly Ala Glu Asn Ser Gly His Val Ile Leu Leu Asp Lys Thr 

                325                 330                 335     

Thr Thr Gly Asp Gly Ile Val Ala Gly Leu Gln Val Leu Ala Ala Met 

            340                 345                 350         

Ala Arg Asn His Met Ser Leu His Asp Leu Cys Ser Gly Met Lys Met 

        355                 360                 365             

Phe Pro Gln Ile Leu Val Asn Val Arg Tyr Thr Ala Gly Ser Gly Asp 

    370                 375                 380                 

Pro Leu Glu His Glu Ser Val Lys Ala Val Thr Ala Glu Val Glu Ala 

385                 390                 395                 400 

Ala Leu Gly Asn Arg Gly Arg Val Leu Leu Arg Lys Ser Gly Thr Glu 

                405                 410                 415     

Pro Leu Ile Arg Val Met Val Glu Gly Glu Asp Glu Ala Gln Val Thr 

            420                 425                 430         

Glu Phe Ala His Arg Ile Ala Asp Ala Val Lys Ala Val 

        435                 440                 445 



<210> 50

<211> 445

<212> PRT

<213> Enterobacteriaceae





<220> 

<223> GlmM, Phosphoglucosamine mutase, GenBank ID: WP_000071134.1



<400> 50

Met Ser Asn Arg Lys Tyr Phe Gly Thr Asp Gly Ile Arg Gly Arg Val 

1               5                   10                  15      

Gly Asp Ala Pro Ile Thr Pro Asp Phe Val Leu Lys Leu Gly Trp Ala 

            20                  25                  30          

Ala Gly Lys Val Leu Ala Arg His Gly Ser Arg Lys Ile Ile Ile Gly 

        35                  40                  45              

Lys Asp Thr Arg Ile Ser Gly Tyr Met Leu Glu Ser Ala Leu Glu Ala 

    50                  55                  60                  

Gly Leu Ala Ala Ala Gly Leu Ser Ala Leu Phe Thr Gly Pro Met Pro 

65                  70                  75                  80  

Thr Pro Ala Val Ala Tyr Leu Thr Arg Thr Phe Arg Ala Glu Ala Gly 

                85                  90                  95      

Ile Val Ile Ser Ala Ser His Asn Pro Phe Tyr Asp Asn Gly Ile Lys 

            100                 105                 110         

Phe Phe Ser Ile Asp Gly Thr Lys Leu Pro Asp Ala Val Glu Glu Ala 

        115                 120                 125             

Ile Glu Ala Glu Met Glu Lys Glu Ile Ser Cys Val Asp Ser Ala Glu 

    130                 135                 140                 

Leu Gly Lys Ala Ser Arg Ile Val Asp Ala Ala Gly Arg Tyr Ile Glu 

145                 150                 155                 160 

Phe Cys Lys Ala Thr Phe Pro Asn Glu Leu Ser Leu Ser Glu Leu Lys 

                165                 170                 175     

Ile Val Val Asp Cys Ala Asn Gly Ala Thr Tyr His Ile Ala Pro Asn 

            180                 185                 190         

Val Leu Arg Glu Leu Gly Ala Asn Val Ile Ala Ile Gly Cys Glu Pro 

        195                 200                 205             

Asn Gly Val Asn Ile Asn Ala Glu Val Gly Ala Thr Asp Val Arg Ala 

    210                 215                 220                 

Leu Gln Ala Arg Val Leu Ala Glu Lys Ala Asp Leu Gly Ile Ala Phe 

225                 230                 235                 240 

Asp Gly Asp Gly Asp Arg Val Ile Met Val Asp His Glu Gly Asn Lys 

                245                 250                 255     

Val Asp Gly Asp Gln Ile Met Tyr Ile Ile Ala Arg Glu Gly Leu Arg 

            260                 265                 270         

Gln Gly Gln Leu Arg Gly Gly Ala Val Gly Thr Leu Met Ser Asn Met 

        275                 280                 285             

Gly Leu Glu Leu Ala Leu Lys Gln Leu Gly Ile Pro Phe Ala Arg Ala 

    290                 295                 300                 

Lys Val Gly Asp Arg Tyr Val Leu Glu Lys Met Gln Glu Lys Gly Trp 

305                 310                 315                 320 

Arg Ile Gly Ala Glu Asn Ser Gly His Val Ile Leu Leu Asp Lys Thr 

                325                 330                 335     

Thr Thr Gly Asp Gly Ile Val Ala Gly Leu Gln Val Leu Ala Ala Met 

            340                 345                 350         

Ala Arg Asn His Met Ser Leu His Asp Leu Cys Ser Gly Met Lys Met 

        355                 360                 365             

Phe Pro Gln Ile Leu Val Asn Val Arg Tyr Thr Ala Gly Ser Gly Asp 

    370                 375                 380                 

Pro Leu Glu His Glu Ser Val Lys Ala Val Thr Ala Glu Val Glu Ala 

385                 390                 395                 400 

Ala Leu Gly Asn Arg Gly Arg Val Leu Leu Arg Lys Ser Gly Thr Glu 

                405                 410                 415     

Pro Leu Ile Arg Val Met Val Glu Gly Glu Asp Glu Ala Gln Val Thr 

            420                 425                 430         

Glu Phe Ala His Arg Ile Ala Asp Ala Val Lys Ala Val 

        435                 440                 445 



<210> 51

<211> 456

<212> PRT

<213> Escherichia coli K-12





<220> 

<223> GlmU, N-acetylglucosamine-1-phosphate uridyltransferase and

      glucosamine-1-phosphate acetyltransferase, GenBank ID:

      NP_418186.1



<400> 51

Met Leu Asn Asn Ala Met Ser Val Val Ile Leu Ala Ala Gly Lys Gly 

1               5                   10                  15      

Thr Arg Met Tyr Ser Asp Leu Pro Lys Val Leu His Thr Leu Ala Gly 

            20                  25                  30          

Lys Ala Met Val Gln His Val Ile Asp Ala Ala Asn Glu Leu Gly Ala 

        35                  40                  45              

Ala His Val His Leu Val Tyr Gly His Gly Gly Asp Leu Leu Lys Gln 

    50                  55                  60                  

Ala Leu Lys Asp Asp Asn Leu Asn Trp Val Leu Gln Ala Glu Gln Leu 

65                  70                  75                  80  

Gly Thr Gly His Ala Met Gln Gln Ala Ala Pro Phe Phe Ala Asp Asp 

                85                  90                  95      

Glu Asp Ile Leu Met Leu Tyr Gly Asp Val Pro Leu Ile Ser Val Glu 

            100                 105                 110         

Thr Leu Gln Arg Leu Arg Asp Ala Lys Pro Gln Gly Gly Ile Gly Leu 

        115                 120                 125             

Leu Thr Val Lys Leu Asp Asp Pro Thr Gly Tyr Gly Arg Ile Thr Arg 

    130                 135                 140                 

Glu Asn Gly Lys Val Thr Gly Ile Val Glu His Lys Asp Ala Thr Asp 

145                 150                 155                 160 

Glu Gln Arg Gln Ile Gln Glu Ile Asn Thr Gly Ile Leu Ile Ala Asn 

                165                 170                 175     

Gly Ala Asp Met Lys Arg Trp Leu Ala Lys Leu Thr Asn Asn Asn Ala 

            180                 185                 190         

Gln Gly Glu Tyr Tyr Ile Thr Asp Ile Ile Ala Leu Ala Tyr Gln Glu 

        195                 200                 205             

Gly Arg Glu Ile Val Ala Val His Pro Gln Arg Leu Ser Glu Val Glu 

    210                 215                 220                 

Gly Val Asn Asn Arg Leu Gln Leu Ser Arg Leu Glu Arg Val Tyr Gln 

225                 230                 235                 240 

Ser Glu Gln Ala Glu Lys Leu Leu Leu Ala Gly Val Met Leu Arg Asp 

                245                 250                 255     

Pro Ala Arg Phe Asp Leu Arg Gly Thr Leu Thr His Gly Arg Asp Val 

            260                 265                 270         

Glu Ile Asp Thr Asn Val Ile Ile Glu Gly Asn Val Thr Leu Gly His 

        275                 280                 285             

Arg Val Lys Ile Gly Thr Gly Cys Val Ile Lys Asn Ser Val Ile Gly 

    290                 295                 300                 

Asp Asp Cys Glu Ile Ser Pro Tyr Thr Val Val Glu Asp Ala Asn Leu 

305                 310                 315                 320 

Ala Ala Ala Cys Thr Ile Gly Pro Phe Ala Arg Leu Arg Pro Gly Ala 

                325                 330                 335     

Glu Leu Leu Glu Gly Ala His Val Gly Asn Phe Val Glu Met Lys Lys 

            340                 345                 350         

Ala Arg Leu Gly Lys Gly Ser Lys Ala Gly His Leu Thr Tyr Leu Gly 

        355                 360                 365             

Asp Ala Glu Ile Gly Asp Asn Val Asn Ile Gly Ala Gly Thr Ile Thr 

    370                 375                 380                 

Cys Asn Tyr Asp Gly Ala Asn Lys Phe Lys Thr Ile Ile Gly Asp Asp 

385                 390                 395                 400 

Val Phe Val Gly Ser Asp Thr Gln Leu Val Ala Pro Val Thr Val Gly 

                405                 410                 415     

Lys Gly Ala Thr Ile Ala Ala Gly Thr Thr Val Thr Arg Asn Val Gly 

            420                 425                 430         

Glu Asn Ala Leu Ala Ile Ser Arg Val Pro Gln Thr Gln Lys Glu Gly 

        435                 440                 445             

Trp Arg Arg Pro Val Lys Lys Lys 

    450                 455     



<210> 52

<211> 456

<212> PRT

<213> Enterobacteriaceae





<220> 

<223> GlmU, N-acetylglucosamine-1-phosphate uridyltransferase and

      glucosamine-1-phosphate acetyltransferase, GenBank ID:

      WP_000933736.1



<400> 52

Met Leu Asn Asn Ala Met Ser Val Val Ile Leu Ala Ala Gly Lys Gly 

1               5                   10                  15      

Thr Arg Met Tyr Ser Asp Leu Pro Lys Val Leu His Thr Leu Ala Gly 

            20                  25                  30          

Lys Ala Met Val Gln His Val Ile Asp Ala Ala Asn Glu Leu Gly Ala 

        35                  40                  45              

Ala His Val His Leu Val Tyr Gly His Gly Gly Asp Leu Leu Lys Gln 

    50                  55                  60                  

Ala Leu Lys Asp Asp Asn Leu Asn Trp Val Leu Gln Ala Glu Gln Leu 

65                  70                  75                  80  

Gly Thr Gly His Ala Met Gln Gln Ala Ala Pro Phe Phe Ala Asp Asp 

                85                  90                  95      

Glu Asp Ile Leu Met Leu Tyr Gly Asp Val Pro Leu Ile Ser Val Glu 

            100                 105                 110         

Thr Leu Gln Arg Leu Arg Asp Ala Lys Pro Gln Gly Gly Ile Gly Leu 

        115                 120                 125             

Leu Thr Val Lys Leu Asp Asp Pro Thr Gly Tyr Gly Arg Ile Thr Arg 

    130                 135                 140                 

Glu Asn Gly Lys Val Thr Gly Ile Val Glu His Lys Asp Ala Thr Asp 

145                 150                 155                 160 

Glu Gln Arg Gln Ile Gln Glu Ile Asn Thr Gly Ile Leu Ile Ala Asn 

                165                 170                 175     

Gly Ala Asp Met Lys Arg Trp Leu Ala Lys Leu Thr Asn Asn Asn Ala 

            180                 185                 190         

Gln Gly Glu Tyr Tyr Ile Thr Asp Ile Ile Ala Leu Ala Tyr Gln Glu 

        195                 200                 205             

Gly Arg Glu Ile Val Ala Val His Pro Gln Arg Leu Ser Glu Val Glu 

    210                 215                 220                 

Gly Val Asn Asn Arg Leu Gln Leu Ser Arg Leu Glu Arg Val Tyr Gln 

225                 230                 235                 240 

Ser Glu Gln Ala Glu Lys Leu Leu Leu Ala Gly Val Met Leu Arg Asp 

                245                 250                 255     

Pro Ala Arg Phe Asp Leu Arg Gly Thr Leu Thr His Gly Arg Asp Val 

            260                 265                 270         

Glu Ile Asp Thr Asn Val Ile Ile Glu Gly Asn Val Thr Leu Gly His 

        275                 280                 285             

Arg Val Lys Ile Gly Thr Gly Cys Val Ile Lys Asn Ser Val Ile Gly 

    290                 295                 300                 

Asp Asp Cys Glu Ile Ser Pro Tyr Thr Val Val Glu Asp Ala Asn Leu 

305                 310                 315                 320 

Ala Ala Ala Cys Thr Ile Gly Pro Phe Ala Arg Leu Arg Pro Gly Ala 

                325                 330                 335     

Glu Leu Leu Glu Gly Ala His Val Gly Asn Phe Val Glu Met Lys Lys 

            340                 345                 350         

Ala Arg Leu Gly Lys Gly Ser Lys Ala Gly His Leu Thr Tyr Leu Gly 

        355                 360                 365             

Asp Ala Glu Ile Gly Asp Asn Val Asn Ile Gly Ala Gly Thr Ile Thr 

    370                 375                 380                 

Cys Asn Tyr Asp Gly Ala Asn Lys Phe Lys Thr Ile Ile Gly Asp Asp 

385                 390                 395                 400 

Val Phe Val Gly Ser Asp Thr Gln Leu Val Ala Pro Val Thr Val Gly 

                405                 410                 415     

Lys Gly Ala Thr Ile Ala Ala Gly Thr Thr Val Thr Arg Asn Val Gly 

            420                 425                 430         

Glu Asn Ala Leu Ala Ile Ser Arg Val Pro Gln Thr Gln Lys Glu Gly 

        435                 440                 445             

Trp Arg Arg Pro Val Lys Lys Lys 

    450                 455     



<210> 53

<211> 609

<212> PRT

<213> Escherichia coli K-12





<220> 

<223> GlmS, L-glutamine--D-fructose-6-phosphate aminotransferase,

      GenBank ID: NP_418185.1



<400> 53

Met Cys Gly Ile Val Gly Ala Ile Ala Gln Arg Asp Val Ala Glu Ile 

1               5                   10                  15      

Leu Leu Glu Gly Leu Arg Arg Leu Glu Tyr Arg Gly Tyr Asp Ser Ala 

            20                  25                  30          

Gly Leu Ala Val Val Asp Ala Glu Gly His Met Thr Arg Leu Arg Arg 

        35                  40                  45              

Leu Gly Lys Val Gln Met Leu Ala Gln Ala Ala Glu Glu His Pro Leu 

    50                  55                  60                  

His Gly Gly Thr Gly Ile Ala His Thr Arg Trp Ala Thr His Gly Glu 

65                  70                  75                  80  

Pro Ser Glu Val Asn Ala His Pro His Val Ser Glu His Ile Val Val 

                85                  90                  95      

Val His Asn Gly Ile Ile Glu Asn His Glu Pro Leu Arg Glu Glu Leu 

            100                 105                 110         

Lys Ala Arg Gly Tyr Thr Phe Val Ser Glu Thr Asp Thr Glu Val Ile 

        115                 120                 125             

Ala His Leu Val Asn Trp Glu Leu Lys Gln Gly Gly Thr Leu Arg Glu 

    130                 135                 140                 

Ala Val Leu Arg Ala Ile Pro Gln Leu Arg Gly Ala Tyr Gly Thr Val 

145                 150                 155                 160 

Ile Met Asp Ser Arg His Pro Asp Thr Leu Leu Ala Ala Arg Ser Gly 

                165                 170                 175     

Ser Pro Leu Val Ile Gly Leu Gly Met Gly Glu Asn Phe Ile Ala Ser 

            180                 185                 190         

Asp Gln Leu Ala Leu Leu Pro Val Thr Arg Arg Phe Ile Phe Leu Glu 

        195                 200                 205             

Glu Gly Asp Ile Ala Glu Ile Thr Arg Arg Ser Val Asn Ile Phe Asp 

    210                 215                 220                 

Lys Thr Gly Ala Glu Val Lys Arg Gln Asp Ile Glu Ser Asn Leu Gln 

225                 230                 235                 240 

Tyr Asp Ala Gly Asp Lys Gly Ile Tyr Arg His Tyr Met Gln Lys Glu 

                245                 250                 255     

Ile Tyr Glu Gln Pro Asn Ala Ile Lys Asn Thr Leu Thr Gly Arg Ile 

            260                 265                 270         

Ser His Gly Gln Val Asp Leu Ser Glu Leu Gly Pro Asn Ala Asp Glu 

        275                 280                 285             

Leu Leu Ser Lys Val Glu His Ile Gln Ile Leu Ala Cys Gly Thr Ser 

    290                 295                 300                 

Tyr Asn Ser Gly Met Val Ser Arg Tyr Trp Phe Glu Ser Leu Ala Gly 

305                 310                 315                 320 

Ile Pro Cys Asp Val Glu Ile Ala Ser Glu Phe Arg Tyr Arg Lys Ser 

                325                 330                 335     

Ala Val Arg Arg Asn Ser Leu Met Ile Thr Leu Ser Gln Ser Gly Glu 

            340                 345                 350         

Thr Ala Asp Thr Leu Ala Gly Leu Arg Leu Ser Lys Glu Leu Gly Tyr 

        355                 360                 365             

Leu Gly Ser Leu Ala Ile Cys Asn Val Pro Gly Ser Ser Leu Val Arg 

    370                 375                 380                 

Glu Ser Asp Leu Ala Leu Met Thr Asn Ala Gly Thr Glu Ile Gly Val 

385                 390                 395                 400 

Ala Ser Thr Lys Ala Phe Thr Thr Gln Leu Thr Val Leu Leu Met Leu 

                405                 410                 415     

Val Ala Lys Leu Ser Arg Leu Lys Gly Leu Asp Ala Ser Ile Glu His 

            420                 425                 430         

Asp Ile Val His Gly Leu Gln Ala Leu Pro Ser Arg Ile Glu Gln Met 

        435                 440                 445             

Leu Ser Gln Asp Lys Arg Ile Glu Ala Leu Ala Glu Asp Phe Ser Asp 

    450                 455                 460                 

Lys His His Ala Leu Phe Leu Gly Arg Gly Asp Gln Tyr Pro Ile Ala 

465                 470                 475                 480 

Leu Glu Gly Ala Leu Lys Leu Lys Glu Ile Ser Tyr Ile His Ala Glu 

                485                 490                 495     

Ala Tyr Ala Ala Gly Glu Leu Lys His Gly Pro Leu Ala Leu Ile Asp 

            500                 505                 510         

Ala Asp Met Pro Val Ile Val Val Ala Pro Asn Asn Glu Leu Leu Glu 

        515                 520                 525             

Lys Leu Lys Ser Asn Ile Glu Glu Val Arg Ala Arg Gly Gly Gln Leu 

    530                 535                 540                 

Tyr Val Phe Ala Asp Gln Asp Ala Gly Phe Val Ser Ser Asp Asn Met 

545                 550                 555                 560 

His Ile Ile Glu Met Pro His Val Glu Glu Val Ile Ala Pro Ile Phe 

                565                 570                 575     

Tyr Thr Val Pro Leu Gln Leu Leu Ala Tyr His Val Ala Leu Ile Lys 

            580                 585                 590         

Gly Thr Asp Val Asp Gln Pro Arg Asn Leu Ala Lys Ser Val Thr Val 

        595                 600                 605             

Glu 

    



<210> 54

<211> 609

<212> PRT

<213> Enterobacteriaceae





<220> 

<223> GlmS, L-glutamine--D-fructose-6-phosphate aminotransferase,

      GenBank ID: WP_000334099.1



<400> 54

Met Cys Gly Ile Val Gly Ala Ile Ala Gln Arg Asp Val Ala Glu Ile 

1               5                   10                  15      

Leu Leu Glu Gly Leu Arg Arg Leu Glu Tyr Arg Gly Tyr Asp Ser Ala 

            20                  25                  30          

Gly Leu Ala Val Val Asp Ala Glu Gly His Met Thr Arg Leu Arg Arg 

        35                  40                  45              

Leu Gly Lys Val Gln Met Leu Ala Gln Ala Ala Glu Glu His Pro Leu 

    50                  55                  60                  

His Gly Gly Thr Gly Ile Ala His Thr Arg Trp Ala Thr His Gly Glu 

65                  70                  75                  80  

Pro Ser Glu Val Asn Ala His Pro His Val Ser Glu His Ile Val Val 

                85                  90                  95      

Val His Asn Gly Ile Ile Glu Asn His Glu Pro Leu Arg Glu Glu Leu 

            100                 105                 110         

Lys Ala Arg Gly Tyr Thr Phe Val Ser Glu Thr Asp Thr Glu Val Ile 

        115                 120                 125             

Ala His Leu Val Asn Trp Glu Leu Lys Gln Gly Gly Thr Leu Arg Glu 

    130                 135                 140                 

Ala Val Leu Arg Ala Ile Pro Gln Leu Arg Gly Ala Tyr Gly Thr Val 

145                 150                 155                 160 

Ile Met Asp Ser Arg His Pro Asp Thr Leu Leu Ala Ala Arg Ser Gly 

                165                 170                 175     

Ser Pro Leu Val Ile Gly Leu Gly Met Gly Glu Asn Phe Ile Ala Ser 

            180                 185                 190         

Asp Gln Leu Ala Leu Leu Pro Val Thr Arg Arg Phe Ile Phe Leu Glu 

        195                 200                 205             

Glu Gly Asp Ile Ala Glu Ile Thr Arg Arg Ser Val Asn Ile Phe Asp 

    210                 215                 220                 

Lys Thr Gly Ala Glu Val Lys Arg Gln Asp Ile Glu Ser Asn Leu Gln 

225                 230                 235                 240 

Tyr Asp Ala Gly Asp Lys Gly Ile Tyr Arg His Tyr Met Gln Lys Glu 

                245                 250                 255     

Ile Tyr Glu Gln Pro Asn Ala Ile Lys Asn Thr Leu Thr Gly Arg Ile 

            260                 265                 270         

Ser His Gly Gln Val Asp Leu Ser Glu Leu Gly Pro Asn Ala Asp Glu 

        275                 280                 285             

Leu Leu Ser Lys Val Glu His Ile Gln Ile Leu Ala Cys Gly Thr Ser 

    290                 295                 300                 

Tyr Asn Ser Gly Met Val Ser Arg Tyr Trp Phe Glu Ser Leu Ala Gly 

305                 310                 315                 320 

Ile Pro Cys Asp Val Glu Ile Ala Ser Glu Phe Arg Tyr Arg Lys Ser 

                325                 330                 335     

Ala Val Arg Arg Asn Ser Leu Met Ile Thr Leu Ser Gln Ser Gly Glu 

            340                 345                 350         

Thr Ala Asp Thr Leu Ala Gly Leu Arg Leu Ser Lys Glu Leu Gly Tyr 

        355                 360                 365             

Leu Gly Ser Leu Ala Ile Cys Asn Val Pro Gly Ser Ser Leu Val Arg 

    370                 375                 380                 

Glu Ser Asp Leu Ala Leu Met Thr Asn Ala Gly Thr Glu Ile Gly Val 

385                 390                 395                 400 

Ala Ser Thr Lys Ala Phe Thr Thr Gln Leu Thr Val Leu Leu Met Leu 

                405                 410                 415     

Val Ala Lys Leu Ser Arg Leu Lys Gly Leu Asp Ala Ser Ile Glu His 

            420                 425                 430         

Asp Ile Val His Gly Leu Gln Ala Leu Pro Ser Arg Ile Glu Gln Met 

        435                 440                 445             

Leu Ser Gln Asp Lys Arg Ile Glu Ala Leu Ala Glu Asp Phe Ser Asp 

    450                 455                 460                 

Lys His His Ala Leu Phe Leu Gly Arg Gly Asp Gln Tyr Pro Ile Ala 

465                 470                 475                 480 

Leu Glu Gly Ala Leu Lys Leu Lys Glu Ile Ser Tyr Ile His Ala Glu 

                485                 490                 495     

Ala Tyr Ala Ala Gly Glu Leu Lys His Gly Pro Leu Ala Leu Ile Asp 

            500                 505                 510         

Ala Asp Met Pro Val Ile Val Val Ala Pro Asn Asn Glu Leu Leu Glu 

        515                 520                 525             

Lys Leu Lys Ser Asn Ile Glu Glu Val Arg Ala Arg Gly Gly Gln Leu 

    530                 535                 540                 

Tyr Val Phe Ala Asp Gln Asp Ala Gly Phe Val Ser Ser Asp Asn Met 

545                 550                 555                 560 

His Ile Ile Glu Met Pro His Val Glu Glu Val Ile Ala Pro Ile Phe 

                565                 570                 575     

Tyr Thr Val Pro Leu Gln Leu Leu Ala Tyr His Val Ala Leu Ile Lys 

            580                 585                 590         

Gly Thr Asp Val Asp Gln Pro Arg Asn Leu Ala Lys Ser Val Thr Val 

        595                 600                 605             

Glu 

    



<210> 55

<211> 221

<212> PRT

<213> Campylobacter jejuni





<220> 

<223> NeuA, CMP-Neu5Ac synthetase, GenBank ID: AAK91728.1



<400> 55

Met Ser Leu Ala Ile Ile Pro Ala Arg Gly Gly Ser Lys Gly Ile Lys 

1               5                   10                  15      

Asn Lys Asn Leu Val Leu Leu Asn Asn Lys Pro Leu Ile Tyr Tyr Thr 

            20                  25                  30          

Ile Lys Ala Ala Leu Asn Ala Lys Ser Ile Ser Lys Val Val Val Ser 

        35                  40                  45              

Ser Asp Ser Asp Glu Ile Leu Asn Tyr Ala Lys Ser Gln Asn Val Asp 

    50                  55                  60                  

Ile Leu Lys Arg Pro Ile Ser Leu Ala Gln Asp Asp Thr Thr Ser Asp 

65                  70                  75                  80  

Lys Val Leu Leu His Ala Leu Lys Phe Tyr Lys Asp Tyr Glu Asp Val 

                85                  90                  95      

Val Phe Leu Gln Pro Thr Ser Pro Leu Arg Thr Asn Ile His Ile Asn 

            100                 105                 110         

Glu Ala Phe Asn Leu Tyr Lys Asn Ser Asn Ala Asn Ala Leu Ile Ser 

        115                 120                 125             

Val Ser Glu Cys Asp Asn Lys Ile Leu Lys Ala Phe Val Cys Asn Asp 

    130                 135                 140                 

Cys Gly Asp Leu Ala Gly Ile Cys Asn Asp Glu Tyr Pro Phe Met Pro 

145                 150                 155                 160 

Arg Gln Lys Leu Pro Lys Thr Tyr Met Ser Asn Gly Ala Ile Tyr Ile 

                165                 170                 175     

Leu Lys Ile Lys Glu Phe Leu Asn Asn Pro Ser Phe Leu Gln Ser Lys 

            180                 185                 190         

Thr Lys His Phe Leu Met Asp Glu Ser Ser Ser Leu Asp Ile Asp Cys 

        195                 200                 205             

Leu Glu Asp Leu Lys Lys Val Glu Gln Ile Trp Lys Lys 

    210                 215                 220     



<210> 56

<211> 231

<212> PRT

<213> Vibrio brasiliensis





<220> 

<223> NeuA, CMP-Neu5Ac synthetase, GenBank ID: WP_006881452.1



<400> 56

Met Arg Val Ala Leu Ile Thr Ala Arg Gly Gly Ser Lys Gly Leu Pro 

1               5                   10                  15      

Arg Lys Asn Val Leu Asn Val Asn Gly Leu Pro Leu Ile Gly Trp Thr 

            20                  25                  30          

Ile Asn Ala Ala Arg Glu Cys Ser Tyr Ile Asp Arg Val Phe Val Ser 

        35                  40                  45              

Thr Glu Asp Glu Glu Ile Ala Lys Ile Ser Leu Gly Met Gly Ala Glu 

    50                  55                  60                  

Leu Ile Glu Arg Pro Cys Glu Leu Ala Gln Asp Asn Ser Thr Ser Asp 

65                  70                  75                  80  

Glu Val Ile Arg His Ala Ile Asp Trp Leu Asp Lys His Ser Ile Leu 

                85                  90                  95      

Ser Asp Leu Val Val Leu Leu Gln Pro Thr Ser Pro Leu Arg Thr Ser 

            100                 105                 110         

Ala His Ile Ser Glu Ala Ile Asp Leu Tyr Asp Ser Leu Asn Asn Pro 

        115                 120                 125             

Asn Ala Thr Ile Ile Ser Val Tyr Glu Pro Glu His Ser Pro Leu Lys 

    130                 135                 140                 

Ala Phe Val Gln Leu Glu Asn Gly Thr Leu Lys Gly Ser Tyr Asn Glu 

145                 150                 155                 160 

Asp Ala Pro Tyr Thr Arg Arg Gln Glu Leu Pro Lys Ser Tyr Leu Ala 

                165                 170                 175     

Asn Gly Ala Ile Tyr Ile Phe Ser Pro Ser Ala Phe Met Ser Glu Ser 

            180                 185                 190         

Gln Ile Pro Arg Arg Asp Ile Tyr Pro Tyr Val Met Ser Thr Asn Glu 

        195                 200                 205             

Ser Glu Asp Ile Asp Ser Lys Ile Asp Leu Ile Arg Val Glu Lys Leu 

    210                 215                 220                 

Leu Arg Gly Ser Phe Tyr Glu 

225                 230     



<210> 57

<211> 346

<212> PRT

<213> Campylobacter jejuni





<220> 

<223> NeuB, Neu5 Ac synthase, GenBank ID: AAK91726.1



<400> 57

Met Lys Glu Ile Lys Ile Gln Asn Ile Ile Ile Ser Glu Glu Lys Ala 

1               5                   10                  15      

Pro Leu Val Val Pro Glu Ile Gly Ile Asn His Asn Gly Ser Leu Glu 

            20                  25                  30          

Leu Ala Lys Ile Met Val Asp Ala Ala Phe Ser Ala Gly Ala Lys Ile 

        35                  40                  45              

Ile Lys His Gln Thr His Ile Val Glu Asp Glu Met Ser Lys Ala Ala 

    50                  55                  60                  

Lys Lys Val Ile Pro Gly Asn Ala Lys Ile Ser Ile Tyr Glu Ile Met 

65                  70                  75                  80  

Gln Lys Cys Ala Leu Asp Tyr Lys Asp Glu Leu Ala Leu Lys Glu Tyr 

                85                  90                  95      

Thr Glu Lys Leu Gly Leu Val Tyr Leu Ser Thr Pro Phe Ser Arg Ala 

            100                 105                 110         

Gly Ala Asn Arg Leu Glu Asp Met Gly Val Ser Ala Phe Lys Ile Gly 

        115                 120                 125             

Ser Gly Glu Cys Asn Asn Tyr Pro Leu Ile Lys His Ile Ala Ala Phe 

    130                 135                 140                 

Lys Lys Pro Met Ile Val Ser Thr Gly Met Asn Ser Ile Glu Ser Ile 

145                 150                 155                 160 

Lys Pro Thr Val Lys Ile Leu Leu Asp Asn Glu Ile Pro Phe Val Leu 

                165                 170                 175     

Met His Thr Thr Asn Leu Tyr Pro Thr Pro His Asn Leu Val Arg Leu 

            180                 185                 190         

Asn Ala Met Leu Glu Leu Lys Lys Glu Phe Ser Cys Met Val Gly Leu 

        195                 200                 205             

Ser Asp His Thr Thr Asp Asn Leu Ala Cys Leu Gly Ala Val Val Leu 

    210                 215                 220                 

Gly Ala Cys Val Leu Glu Arg His Phe Thr Asp Ser Met His Arg Ser 

225                 230                 235                 240 

Gly Pro Asp Ile Val Cys Ser Met Asp Thr Lys Ala Leu Lys Glu Leu 

                245                 250                 255     

Ile Ile Gln Ser Glu Gln Met Ala Ile Ile Arg Gly Asn Asn Glu Ser 

            260                 265                 270         

Lys Lys Ala Ala Lys Gln Glu Gln Val Thr Ile Asp Phe Ala Phe Ala 

        275                 280                 285             

Ser Val Val Ser Ile Lys Asp Ile Lys Lys Gly Glu Val Leu Ser Met 

    290                 295                 300                 

Asp Asn Ile Trp Val Lys Arg Pro Gly Leu Gly Gly Ile Ser Ala Ala 

305                 310                 315                 320 

Glu Phe Glu Asn Ile Leu Gly Lys Lys Ala Leu Arg Asp Ile Glu Asn 

                325                 330                 335     

Asp Ala Gln Leu Ser Tyr Glu Asp Phe Ala 

            340                 345     



<210> 58

<211> 346

<212> PRT

<213> Flavobacterium limnosediminis





<220> 

<223> NeuB, Neu5 Ac synthase, GenBank ID: WP_023580510.1



<400> 58

Met Asn Pro Tyr Ile Glu Ile Ala Gly Arg Lys Ile Gly Leu Asp Tyr 

1               5                   10                  15      

Pro Pro Leu Val Ile Ala Glu Ile Gly Ile Asn His Glu Gly Ser Leu 

            20                  25                  30          

Gln Val Ala Lys Glu Ile Val Asp Ala Ala Phe Arg Ala Gly Val Glu 

        35                  40                  45              

Val Val Lys His Gln Thr His Ile Val Glu Asp Glu Met Ser Gly Ala 

    50                  55                  60                  

Ala Lys Lys Val Ile Pro Gly Asn Ala Asp Val Ser Ile Tyr Glu Ile 

65                  70                  75                  80  

Met Glu Arg Cys Ala Leu Asp Glu Ser Glu Glu Leu Glu Leu Lys Asn 

                85                  90                  95      

Tyr Val Glu Ser Lys Gly Met Ile Phe Ile Ser Thr Pro Phe Ser Arg 

            100                 105                 110         

Ala Ala Ala Glu Arg Leu Lys Lys Phe Asp Ile Pro Ala Tyr Lys Ile 

        115                 120                 125             

Gly Ser Gly Glu Cys Asn Asn Tyr Pro Leu Leu Glu His Ile Ala Ser 

    130                 135                 140                 

Phe Gly Lys Pro Val Ile Leu Ser Thr Gly Met Asn Thr Val Asp Ser 

145                 150                 155                 160 

Ile Arg Lys Ala Val Ala Ile Phe Asp Lys His Asn Val Pro Val Ala 

                165                 170                 175     

Leu Leu His Thr Thr Asn Leu Tyr Pro Thr Pro Ile His Leu Val Arg 

            180                 185                 190         

Phe Gly Ala Met Met Glu Met His Asn Ala Phe Pro Asp Lys Val Phe 

        195                 200                 205             

Gly Leu Ser Asp His Thr Leu Asn Asn Asn Ala Cys Leu Gly Ala Val 

    210                 215                 220                 

Ala Leu Gly Ala Ser Ile Leu Glu Arg His Phe Thr Asp His Met Ser 

225                 230                 235                 240 

Arg Thr Gly Pro Asp Ile Val Cys Ser Met Asp Glu Gln Ala Thr Arg 

                245                 250                 255     

Glu Leu Ile Val Asn Ser Ala Glu Ile Ala Leu Met Arg Gly Gly Thr 

            260                 265                 270         

Lys Lys Pro Ala Ala Glu Glu Gln Val Thr Ile Asp Phe Ala Phe Ala 

        275                 280                 285             

Thr Val Cys Ser Ile Ala Pro Ile Lys Lys Gly Glu Val Phe Thr Lys 

    290                 295                 300                 

Glu Asn Ile Trp Val Lys Arg Pro Gly Thr Gly Lys Ile Leu Ala Glu 

305                 310                 315                 320 

His Phe Glu Arg Leu Leu Gly Lys Thr Ala Thr Arg Asp Ile Glu Asn 

                325                 330                 335     

Asp Glu Gln Leu Asp Phe Ser Asp Val Glu 

            340                 345     



<210> 59

<211> 372

<212> PRT

<213> Campylobacter jejuni





<220> 

<223> NeuC, UDP-GlcNAc 2-epimerase, GenBank AAK91727.1



<400> 59

Met Lys Lys Ile Leu Phe Ile Thr Gly Ser Arg Ala Asp Tyr Ser Lys 

1               5                   10                  15      

Ile Lys Ser Leu Met Tyr Arg Val Gln Asn Ser Ser Glu Phe Glu Leu 

            20                  25                  30          

Tyr Ile Phe Ala Thr Gly Met His Leu Ser Lys Asn Phe Gly Tyr Thr 

        35                  40                  45              

Val Lys Glu Leu Tyr Lys Asn Gly Phe Lys Asn Ile Tyr Glu Phe Ile 

    50                  55                  60                  

Asn Tyr Asp Lys Tyr Tyr Gln Thr Asp Lys Ala Leu Ala Thr Thr Ile 

65                  70                  75                  80  

Asp Gly Phe Ser Arg Tyr Ala Asn Glu Leu Lys Pro Asp Leu Ile Val 

                85                  90                  95      

Val His Gly Asp Arg Ile Glu Pro Leu Ala Ala Ala Ile Val Gly Ala 

            100                 105                 110         

Leu Asn Asn Ile Leu Val Ala His Ile Glu Gly Gly Glu Ile Ser Gly 

        115                 120                 125             

Thr Ile Asp Asp Ser Leu Arg His Ala Ile Ser Lys Leu Ala His Ile 

    130                 135                 140                 

His Leu Val Asn Asp Glu Phe Ala Lys Arg Arg Leu Met Gln Leu Gly 

145                 150                 155                 160 

Glu Asp Glu Lys Ser Ile Phe Ile Ile Gly Ser Pro Asp Leu Glu Leu 

                165                 170                 175     

Leu Asn Asp Asn Lys Ile Ser Leu Ser Glu Ala Lys Lys Tyr Tyr Asp 

            180                 185                 190         

Ile Asn Tyr Glu Asn Tyr Ala Leu Leu Met Phe His Pro Val Thr Thr 

        195                 200                 205             

Glu Ile Thr Ser Ile Lys Asn Gln Ala Asp Asn Leu Val Lys Ala Leu 

    210                 215                 220                 

Ile Gln Ser Asn Lys Asn Tyr Ile Val Ile Tyr Pro Asn Asn Asp Leu 

225                 230                 235                 240 

Gly Phe Glu Leu Ile Leu Gln Ser Tyr Glu Glu Phe Lys Asn Asn Pro 

                245                 250                 255     

Arg Phe Lys Leu Phe Pro Ser Leu Arg Phe Glu Tyr Phe Ile Thr Leu 

            260                 265                 270         

Leu Lys Asn Ala Asp Phe Ile Ile Gly Asn Ser Ser Cys Ile Leu Lys 

        275                 280                 285             

Glu Ala Leu Tyr Leu Lys Thr Ala Gly Ile Leu Val Gly Ser Arg Gln 

    290                 295                 300                 

Asn Gly Arg Leu Gly Asn Glu Asn Thr Leu Lys Val Asn Ala Asn Ser 

305                 310                 315                 320 

Asp Glu Ile Leu Lys Ala Ile Asn Thr Ile His Lys Lys Gln Asp Leu 

                325                 330                 335     

Phe Ser Ala Lys Leu Glu Ile Leu Asp Ser Ser Lys Leu Phe Phe Glu 

            340                 345                 350         

Tyr Leu Gln Ser Gly Asp Phe Phe Lys Leu Ser Thr Gln Lys Val Phe 

        355                 360                 365             

Lys Asp Ile Lys 

    370         



<210> 60

<211> 391

<212> PRT

<213> Enterobacteriaceae





<220> 

<223> NeuC, UDP-GlcNAc 2-epimerase, GenBank ID: WP_000723250.1



<400> 60

Met Lys Lys Ile Leu Tyr Val Thr Gly Ser Arg Ala Glu Tyr Gly Ile 

1               5                   10                  15      

Val Arg Arg Leu Leu Thr Met Leu Arg Glu Thr Pro Glu Ile Gln Leu 

            20                  25                  30          

Asp Leu Ala Val Thr Gly Met His Cys Asp Asn Ala Tyr Gly Asn Thr 

        35                  40                  45              

Ile His Ile Ile Glu Gln Asp Asn Phe Asn Ile Ile Lys Val Val Asp 

    50                  55                  60                  

Ile Asn Ile Asn Thr Thr Ser His Thr His Ile Leu His Ser Met Ser 

65                  70                  75                  80  

Val Cys Leu Asn Ser Phe Gly Asp Phe Phe Ser Asn Asn Thr Tyr Asp 

                85                  90                  95      

Ala Val Met Val Leu Gly Asp Arg Tyr Glu Ile Phe Ser Val Ala Ile 

            100                 105                 110         

Ala Ala Ser Met His Asn Ile Pro Leu Ile His Ile His Gly Gly Glu 

        115                 120                 125             

Lys Thr Leu Ala Asn Tyr Asp Glu Phe Ile Arg His Ser Ile Thr Lys 

    130                 135                 140                 

Met Ser Lys Leu His Leu Thr Ser Thr Glu Glu Tyr Lys Lys Arg Val 

145                 150                 155                 160 

Ile Gln Leu Gly Glu Lys Pro Gly Ser Val Phe Asn Ile Gly Ser Leu 

                165                 170                 175     

Gly Ala Glu Asn Ala Leu Ser Leu His Leu Pro Asn Lys Gln Glu Leu 

            180                 185                 190         

Glu Leu Lys Tyr Gly Ser Leu Leu Lys Arg Tyr Phe Val Val Val Phe 

        195                 200                 205             

His Pro Glu Thr Leu Ser Thr Gln Ser Val Asn Asp Gln Ile Asp Glu 

    210                 215                 220                 

Leu Leu Ser Ala Ile Ser Phe Phe Lys Asn Thr His Asp Phe Ile Phe 

225                 230                 235                 240 

Ile Gly Ser Asn Ala Asp Thr Gly Ser Asp Ile Ile Gln Arg Lys Val 

                245                 250                 255     

Lys Tyr Phe Cys Lys Glu Tyr Lys Phe Arg Tyr Leu Ile Ser Ile Arg 

            260                 265                 270         

Ser Glu Asp Tyr Leu Ala Met Ile Lys Tyr Ser Cys Gly Leu Ile Gly 

        275                 280                 285             

Asn Ser Ser Ser Gly Leu Ile Glu Val Pro Ser Leu Lys Val Ala Thr 

    290                 295                 300                 

Ile Asn Ile Gly Asp Arg Gln Lys Gly Arg Val Arg Gly Ala Ser Val 

305                 310                 315                 320 

Ile Asp Val Pro Val Glu Lys Asn Ala Ile Val Arg Gly Ile Asn Ile 

                325                 330                 335     

Ser Gln Asp Glu Lys Phe Ile Ser Val Val Gln Ser Ser Ser Asn Pro 

            340                 345                 350         

Tyr Phe Lys Glu Asn Ala Leu Ile Asn Ala Val Arg Ile Ile Lys Asp 

        355                 360                 365             

Phe Ile Lys Ser Lys Asn Lys Asp Tyr Lys Asp Phe Tyr Asp Ile Pro 

    370                 375                 380                 

Glu Cys Thr Thr Ser Tyr Asp 

385                 390     



<210> 61

<211> 391

<212> PRT

<213> Escherichia coli S88





<220> 

<223> NeuC, UDP-GlcNAc 2-epimerase, GenBank ID: CAR04561.1



<400> 61

Met Lys Lys Ile Leu Tyr Val Thr Gly Ser Arg Ala Glu Tyr Gly Ile 

1               5                   10                  15      

Val Arg Arg Leu Leu Thr Met Leu Arg Glu Thr Pro Glu Ile Gln Leu 

            20                  25                  30          

Asp Leu Ala Val Thr Gly Met His Cys Asp Asn Ala Tyr Gly Asn Thr 

        35                  40                  45              

Ile His Ile Ile Glu Gln Asp Asn Phe Asn Ile Ile Lys Val Val Asp 

    50                  55                  60                  

Ile Asn Ile Asn Thr Thr Ser His Thr His Ile Leu His Ser Met Ser 

65                  70                  75                  80  

Val Cys Leu Asn Ser Phe Gly Asp Phe Phe Ser Asn Asn Thr Tyr Asp 

                85                  90                  95      

Ala Val Met Val Leu Gly Asp Arg Tyr Glu Ile Phe Ser Val Ala Ile 

            100                 105                 110         

Ala Ala Ser Met His Asn Ile Pro Leu Ile His Ile His Gly Gly Glu 

        115                 120                 125             

Lys Thr Leu Ala Asn Tyr Asp Glu Phe Ile Arg His Ser Ile Thr Lys 

    130                 135                 140                 

Met Ser Lys Leu His Leu Thr Ser Thr Glu Glu Tyr Lys Lys Arg Val 

145                 150                 155                 160 

Ile Gln Leu Gly Glu Lys Pro Gly Ser Val Phe Asn Ile Gly Ser Leu 

                165                 170                 175     

Gly Ala Glu Asn Ala Leu Ser Leu His Leu Pro Asn Lys Gln Glu Leu 

            180                 185                 190         

Glu Leu Lys Tyr Gly Ser Leu Leu Lys Arg Tyr Phe Val Val Val Phe 

        195                 200                 205             

His Pro Glu Thr Leu Ser Thr Gln Ser Val Asn Asp Gln Ile Asp Glu 

    210                 215                 220                 

Leu Leu Ser Ala Ile Ser Phe Phe Lys Asn Thr His Asp Phe Ile Phe 

225                 230                 235                 240 

Ile Gly Ser Asn Ala Asp Thr Gly Ser Asp Ile Ile Gln Arg Lys Val 

                245                 250                 255     

Lys Tyr Phe Cys Lys Glu Tyr Lys Phe Arg Tyr Leu Ile Ser Ile Arg 

            260                 265                 270         

Ser Glu Asp Tyr Leu Ala Met Ile Lys Tyr Ser Cys Gly Leu Ile Gly 

        275                 280                 285             

Asn Ser Ser Ser Gly Leu Ile Glu Val Pro Ser Leu Lys Val Ala Thr 

    290                 295                 300                 

Ile Asn Ile Gly Asp Arg Gln Lys Gly Arg Val Arg Gly Ala Ser Val 

305                 310                 315                 320 

Ile Asp Val Pro Val Glu Lys Asn Ala Ile Val Arg Gly Ile Asn Ile 

                325                 330                 335     

Ser Gln Asp Glu Lys Phe Ile Ser Val Val Gln Ser Ser Ser Asn Pro 

            340                 345                 350         

Tyr Phe Lys Glu Asn Ala Leu Ile Asn Ala Val Arg Ile Ile Lys Asp 

        355                 360                 365             

Phe Ile Lys Ser Lys Asn Lys Asp Tyr Lys Asp Phe Tyr Asp Ile Pro 

    370                 375                 380                 

Glu Cys Thr Thr Ser Tyr Asp 

385                 390     



<210> 62

<211> 392

<212> PRT

<213> Pantoea vagans





<220> 

<223> Vag MFS transporter, WP_048785139.1



<400> 62

Met Lys Ser Leu Leu Thr Arg Lys Arg Arg Ile Asn Pro Val Phe Leu 

1               5                   10                  15      

Ala Phe Met Ala Ala Ser Phe Met Ile Gly Val Ala Gly Ala Leu Gln 

            20                  25                  30          

Ala Pro Thr Leu Ser Leu Phe Leu Thr Arg Glu Val Gln Ala Arg Pro 

        35                  40                  45              

Leu Trp Val Gly Leu Phe Phe Thr Val Asn Ala Ile Ala Gly Ile Val 

    50                  55                  60                  

Val Ser Met Leu Val Ala Lys Arg Ser Asp Ser Arg Gly Asp Arg Arg 

65                  70                  75                  80  

Thr Leu Ile Leu Phe Cys Cys Ala Met Ala Phe Cys Asn Ala Leu Leu 

                85                  90                  95      

Phe Ala Phe Thr Arg His Tyr Leu Thr Leu Ile Thr Leu Gly Val Leu 

            100                 105                 110         

Leu Ser Ala Leu Ala Ser Val Ser Met Pro Gln Ile Phe Ala Leu Ala 

        115                 120                 125             

Arg Glu Tyr Ala Asp Gln Ser Ala Arg Glu Ala Val Met Phe Ser Ser 

    130                 135                 140                 

Val Met Arg Ala Gln Leu Ser Leu Ala Trp Val Ile Gly Pro Pro Leu 

145                 150                 155                 160 

Ser Phe Ala Leu Ala Leu Asn Phe Gly Phe Val Thr Leu Phe Leu Val 

                165                 170                 175     

Ala Ala Ala Leu Phe Leu Val Cys Ile Leu Leu Ile Lys Phe Thr Leu 

            180                 185                 190         

Pro Ser Val Pro Arg Ala Glu Pro Leu Met Arg Ser Gly Gly Met Pro 

        195                 200                 205             

Leu Ser Gly Trp Arg Asp Arg Asp Val Arg Leu Leu Phe Ile Ala Ser 

    210                 215                 220                 

Val Thr Met Trp Thr Cys Asn Thr Met Tyr Ile Ile Asp Met Pro Leu 

225                 230                 235                 240 

Tyr Ile Ser Val Thr Leu Gly Leu Pro Glu Lys Leu Ala Gly Leu Leu 

                245                 250                 255     

Met Gly Thr Ala Ala Gly Leu Glu Ile Pro Val Met Leu Leu Ala Gly 

            260                 265                 270         

His Tyr Ala Lys Arg Val Gly Lys Arg Asn Leu Met Leu Ile Ala Val 

        275                 280                 285             

Ala Ala Gly Val Leu Phe Tyr Ala Gly Leu Ala Met Phe Ala Ser Gln 

    290                 295                 300                 

Thr Ala Leu Met Ala Leu Gln Leu Phe Asn Ala Val Phe Ile Gly Ile 

305                 310                 315                 320 

Ile Ala Gly Ile Gly Met Leu Trp Phe Gln Asp Leu Met Pro Gly Arg 

                325                 330                 335     

Pro Gly Ala Ala Thr Thr Met Phe Thr Asn Ser Ile Ser Thr Gly Met 

            340                 345                 350         

Ile Leu Ala Gly Val Ile Gln Gly Thr Leu Ser Glu Arg Phe Gly His 

        355                 360                 365             

Ile Ala Val Tyr Trp Leu Ala Leu Gly Leu Ala Val Ala Ala Phe Ala 

    370                 375                 380                 

Met Ser Ala Arg Val Lys Asn Val 

385                 390         



<210> 63

<211> 393

<212> PRT

<213> Yersinia frederiksenii





<220> 

<223> Fred MFS transporter, WP_087817556.1



<400> 63

Met Lys Ser Ala Leu Thr Phe Ser Arg Arg Ile Asn Pro Val Phe Leu 

1               5                   10                  15      

Ala Phe Phe Val Val Ala Phe Leu Ser Gly Ile Ala Gly Ala Leu Gln 

            20                  25                  30          

Ala Pro Thr Leu Ser Leu Phe Leu Ser Thr Glu Val Lys Val Arg Pro 

        35                  40                  45              

Leu Trp Val Gly Leu Phe Tyr Thr Val Asn Ala Ile Ala Gly Ile Thr 

    50                  55                  60                  

Val Ser Phe Val Leu Ala Lys Arg Ser Asp Leu Arg Gly Asp Arg Arg 

65                  70                  75                  80  

Lys Leu Ile Leu Val Cys Tyr Leu Met Ala Val Gly Asn Cys Leu Leu 

                85                  90                  95      

Phe Ala Phe Asn Arg Asp Tyr Leu Thr Leu Ile Thr Ala Gly Val Leu 

            100                 105                 110         

Leu Ala Ala Val Ala Asn Thr Ala Met Pro Gln Ile Phe Ala Leu Ala 

        115                 120                 125             

Arg Glu Tyr Ala Asp Asn Ser Ala Arg Glu Val Val Met Phe Ser Ser 

    130                 135                 140                 

Ile Met Arg Ala Gln Leu Ser Leu Ala Trp Val Ile Gly Pro Pro Leu 

145                 150                 155                 160 

Ser Phe Met Leu Ala Leu Asn Tyr Gly Phe Thr Leu Met Phe Cys Ile 

                165                 170                 175     

Ala Ala Gly Ile Phe Val Leu Ser Ala Leu Val Val Trp Phe Ile Leu 

            180                 185                 190         

Pro Ser Val Gln Arg Ala Glu Pro Val Met Asp Ala Pro Thr Val Ala 

        195                 200                 205             

Gln Gly Ser Leu Phe Ala Asp Lys Asp Val Leu Leu Leu Phe Ile Ala 

    210                 215                 220                 

Ser Met Leu Met Trp Thr Cys Asn Thr Met Tyr Ile Ile Asp Met Pro 

225                 230                 235                 240 

Leu Tyr Ile Thr Ala Ser Leu Gly Leu Pro Glu Arg Leu Ala Gly Leu 

                245                 250                 255     

Leu Met Gly Thr Ala Ala Gly Leu Glu Ile Pro Ile Met Leu Leu Ala 

            260                 265                 270         

Gly Tyr Ser Val Arg Arg Phe Gly Lys Arg Lys Ile Met Leu Phe Ala 

        275                 280                 285             

Val Leu Ala Gly Val Leu Phe Tyr Thr Gly Leu Val Leu Phe Lys Phe 

    290                 295                 300                 

Lys Ser Ala Leu Met Leu Leu Gln Ile Phe Asn Ala Ile Phe Ile Gly 

305                 310                 315                 320 

Ile Val Ala Gly Ile Gly Met Leu Tyr Phe Gln Asp Leu Met Pro Gly 

                325                 330                 335     

Arg Ala Gly Ala Ala Thr Thr Leu Phe Thr Asn Ser Ile Ser Thr Gly 

            340                 345                 350         

Val Ile Leu Ala Gly Val Leu Gln Gly Val Leu Thr Glu Thr Trp Gly 

        355                 360                 365             

His Asn Ser Val Tyr Val Met Ala Met Ile Leu Ala Ile Leu Ser Leu 

    370                 375                 380                 

Ile Ile Cys Ala Arg Val Arg Glu Ala 

385                 390             



<210> 64

<211> 398

<212> PRT

<213> Serratia marcescens





<220> 

<223> Marc MFS transporter, WP_060448169.1



<400> 64

Met Gln Arg Leu Ser Arg Leu Ser Leu Arg Ile Asn Pro Ile Phe Ala 

1               5                   10                  15      

Ala Phe Leu Leu Ile Ala Phe Leu Ser Gly Ile Ala Gly Ala Leu Leu 

            20                  25                  30          

Thr Pro Thr Leu Ser Leu Phe Leu Thr Thr Glu Val Lys Val Arg Pro 

        35                  40                  45              

Leu Trp Val Gly Leu Phe Tyr Thr Ala Asn Ala Val Ala Gly Ile Val 

    50                  55                  60                  

Val Ser Phe Leu Leu Ala Lys Arg Ser Asp Thr Arg Gly Asp Arg Arg 

65                  70                  75                  80  

Arg Leu Ile Leu Leu Cys Cys Leu Met Ala Val Gly Asn Cys Leu Leu 

                85                  90                  95      

Phe Ala Phe Asn Arg Asp Tyr Leu Thr Leu Ile Thr Ala Gly Val Leu 

            100                 105                 110         

Met Ser Ala Val Ala Asn Thr Ala Met Pro Gln Ile Phe Ala Leu Ala 

        115                 120                 125             

Arg Glu Tyr Ala Asp Ser Glu Ala Arg Glu Val Val Met Phe Ser Ser 

    130                 135                 140                 

Val Met Arg Ala Gln Leu Ser Leu Ala Trp Val Ile Gly Pro Pro Leu 

145                 150                 155                 160 

Ser Phe Ala Leu Ala Leu Asn Tyr Gly Phe Thr Val Met Phe Leu Ile 

                165                 170                 175     

Ala Ala Val Thr Phe Ala Val Cys Val Leu Leu Val Gly Phe Met Leu 

            180                 185                 190         

Pro Ser Val Pro Arg Ala Ala Glu Asn Glu Gly Leu Gln Gly Gly Val 

        195                 200                 205             

Ser Ala Pro Ile Ala Pro Ala Ser Ala Trp Arg Asn Arg Asp Val Arg 

    210                 215                 220                 

Leu Leu Phe Ile Ala Ser Met Leu Met Trp Thr Cys Asn Thr Leu Tyr 

225                 230                 235                 240 

Ile Ile Asp Met Pro Leu Tyr Ile Thr Ala Asp Leu Gly Leu Pro Glu 

                245                 250                 255     

Gly Leu Ala Gly Val Leu Met Gly Thr Ala Ala Gly Leu Glu Ile Pro 

            260                 265                 270         

Ala Met Leu Leu Ala Gly Tyr Tyr Val Lys Arg Phe Gly Lys Arg Asn 

        275                 280                 285             

Met Met Leu Leu Ala Val Val Ala Gly Val Leu Phe Tyr Leu Gly Leu 

    290                 295                 300                 

Thr Val Leu Glu Ser Lys Pro Ala Leu Ile Ala Leu Gln Leu Leu Asn 

305                 310                 315                 320 

Ala Val Phe Ile Gly Ile Val Ala Gly Ile Gly Met Leu Tyr Phe Gln 

                325                 330                 335     

Asp Leu Met Pro Gly Arg Pro Gly Ala Ala Thr Thr Leu Phe Thr Asn 

            340                 345                 350         

Ser Ile Ser Thr Gly Val Ile Leu Ala Gly Val Leu Gln Gly Ala Leu 

        355                 360                 365             

Val Glu Asn Leu Gly His Gly Ser Val Tyr Trp Met Ala Ala Leu Leu 

    370                 375                 380                 

Ala Leu Ala Ala Leu Gly Met Ser Ala Lys Val Arg Glu Val 

385                 390                 395             



<210> 65

<211> 387

<212> PRT

<213> Rouxiella badensis





<220> 

<223> Bad MFS transporter, WP_017489914.1



<400> 65

Met Ser Ser Arg Arg Leu Ser Ile Ile Phe Ala Thr Phe Leu Leu Val 

1               5                   10                  15      

Ser Phe Leu Thr Gly Ile Ala Gly Ala Leu Gln Ala Pro Thr Leu Ser 

            20                  25                  30          

Leu Phe Leu Thr Asn Glu Val Lys Val Arg Pro Leu Trp Val Gly Leu 

        35                  40                  45              

Phe Tyr Thr Val Asn Ala Leu Gly Gly Ile Val Ile Ser Phe Leu Leu 

    50                  55                  60                  

Ala Asn Tyr Ser Asp Lys Lys Gly Asp Arg Arg Lys Leu Leu Phe Phe 

65                  70                  75                  80  

Cys Thr Leu Met Ala Ile Gly Asn Ser Leu Ile Phe Ala Tyr Ser Arg 

                85                  90                  95      

Asp Tyr Leu Val Leu Ile Ser Val Gly Val Leu Leu Ala Ala Ile Gly 

            100                 105                 110         

Asn Ala Ser Met Pro Gln Leu Phe Ala Leu Ala Arg Glu Tyr Ala Asp 

        115                 120                 125             

Arg Ser Ala His Glu Val Val Met Phe Ser Ser Met Met Arg Ala Thr 

    130                 135                 140                 

Leu Ser Leu Ala Trp Val Leu Gly Pro Pro Ile Ser Phe Thr Leu Ala 

145                 150                 155                 160 

Leu Asn Tyr Gly Phe Thr Leu Met Tyr Leu Cys Ala Ala Gly Val Phe 

                165                 170                 175     

Ile Phe Ser Ala Leu Met Val Trp Phe Phe Leu Pro Ser Val Gly Arg 

            180                 185                 190         

Ile Glu Gln Pro Val Asp Lys Val Val Val His Val Ser Ala Trp Lys 

        195                 200                 205             

Asn Arg Asp Val Arg Leu Leu Phe Phe Ala Ser Leu Leu Met Trp Thr 

    210                 215                 220                 

Cys Asn Ile Met Tyr Ile Ile Asp Met Pro Leu Tyr Ile Thr Ser Asp 

225                 230                 235                 240 

Leu Gly Leu Pro Glu Gly Leu Ala Gly Leu Leu Met Gly Ala Ala Ala 

                245                 250                 255     

Gly Leu Glu Ile Pro Val Met Leu Ile Ala Gly Tyr Leu Val Lys Arg 

            260                 265                 270         

Thr Gly Lys Arg Arg Leu Met Leu Cys Ala Ala Val Phe Gly Ile Leu 

        275                 280                 285             

Phe Tyr Leu Gly Leu Val Leu Phe Gln Phe Lys Ala Ala Leu Met Ile 

    290                 295                 300                 

Leu Gln Leu Phe Asn Ala Ile Phe Ile Gly Ile Ile Ala Gly Ile Gly 

305                 310                 315                 320 

Met Leu Tyr Phe Gln Asp Leu Met Pro Gly Arg Ala Gly Ser Ala Thr 

                325                 330                 335     

Thr Leu Phe Thr Asn Ser Ile Ser Thr Gly Ala Ile Leu Ala Gly Val 

            340                 345                 350         

Ile Gln Gly Thr Ile Val Gln Asn Phe Gly His Tyr Gln Val Tyr Trp 

        355                 360                 365             

Met Ala Leu Ala Leu Ala Val Gly Ala Leu Val Leu Met Thr Arg Val 

    370                 375                 380                 

Lys Asn Val 

385         



<210> 66

<211> 394

<212> PRT

<213> Rosenbergiella nectarea





<220> 

<223> Nec MFS transporter, WP_092672081.1



<400> 66

Met Gln Ser Phe Thr Pro Pro Ala Pro Lys Gly Gly Asn Pro Val Phe 

1               5                   10                  15      

Met Met Phe Met Leu Val Thr Phe Phe Val Ser Ile Ala Gly Ala Leu 

            20                  25                  30          

Gln Ala Pro Thr Leu Ser Leu Tyr Leu Ser Gln Glu Leu Ala Ala Lys 

        35                  40                  45              

Pro Phe Met Val Gly Leu Phe Phe Thr Ile Asn Ala Val Thr Gly Ile 

    50                  55                  60                  

Ile Ile Ser Phe Ile Leu Ala Lys Arg Ser Asp Arg Lys Gly Asp Arg 

65                  70                  75                  80  

Arg Arg Leu Leu Met Phe Cys Cys Ala Met Ala Ile Ala Asn Ala Leu 

                85                  90                  95      

Met Phe Ala Phe Val Arg Gln Tyr Val Val Leu Ile Thr Leu Gly Leu 

            100                 105                 110         

Ile Leu Ser Ala Leu Thr Ser Val Val Met Pro Gln Leu Phe Ala Leu 

        115                 120                 125             

Ala Arg Glu Tyr Ala Asp Arg Thr Gly Arg Glu Val Val Met Phe Ser 

    130                 135                 140                 

Ser Val Met Arg Thr Gln Met Ser Leu Ala Trp Val Ile Gly Pro Pro 

145                 150                 155                 160 

Ile Ser Phe Ala Leu Ala Leu Asn Tyr Gly Phe Ile Thr Leu Tyr Leu 

                165                 170                 175     

Val Ala Ala Ala Leu Phe Leu Leu Ser Leu Ile Leu Ile Lys Thr Thr 

            180                 185                 190         

Leu Pro Ser Val Pro Arg Leu Tyr Pro Ala Glu Asp Leu Ala Lys Ser 

        195                 200                 205             

Ala Ala Ser Gly Trp Lys Arg Thr Asp Val Arg Phe Leu Phe Ala Ala 

    210                 215                 220                 

Ser Val Leu Met Trp Val Cys Asn Leu Met Tyr Ile Ile Asp Met Pro 

225                 230                 235                 240 

Leu Tyr Ile Ser Lys Ser Leu Gly Met Pro Glu Ser Phe Ala Gly Val 

                245                 250                 255     

Leu Met Gly Thr Ala Ala Gly Leu Glu Ile Pro Val Met Leu Leu Ala 

            260                 265                 270         

Gly Tyr Leu Ala Lys Arg Val Gly Lys Arg Pro Leu Val Ile Val Ala 

        275                 280                 285             

Ala Val Cys Gly Leu Ala Phe Tyr Pro Ala Met Leu Val Phe His Gln 

    290                 295                 300                 

Gln Thr Gly Leu Leu Ile Ile Gln Leu Leu Asn Ala Val Phe Ile Gly 

305                 310                 315                 320 

Ile Val Ala Gly Leu Val Met Leu Trp Phe Gln Asp Leu Met Pro Gly 

                325                 330                 335     

Lys Ala Gly Ala Ala Thr Thr Leu Phe Thr Asn Ser Val Ser Thr Gly 

            340                 345                 350         

Met Ile Phe Ala Gly Leu Cys Gln Gly Leu Leu Ser Asp Leu Leu Gly 

        355                 360                 365             

His Gln Ala Ile Tyr Val Leu Ala Thr Val Leu Met Val Ile Ala Leu 

    370                 375                 380                 

Leu Leu Leu Leu Arg Val Lys Glu Gln Ala 

385                 390                 



<210> 67

<211> 393

<212> PRT

<213> Yersinia bercovieri





<220> 

<223> YberC MFS transporter



<400> 67

Met Lys Ser Ala Leu Thr Phe Ser Arg Arg Ile Asn Pro Val Phe Leu 

1               5                   10                  15      

Ala Phe Phe Val Val Ala Phe Leu Ser Gly Ile Ala Gly Ala Leu Gln 

            20                  25                  30          

Ala Pro Thr Leu Ser Leu Phe Leu Ser Thr Glu Val Lys Val Arg Pro 

        35                  40                  45              

Leu Trp Val Gly Leu Phe Tyr Thr Val Asn Ala Ile Ala Gly Ile Thr 

    50                  55                  60                  

Val Ser Phe Ile Leu Ala Lys Arg Ser Asp Ser Arg Gly Asp Arg Arg 

65                  70                  75                  80  

Lys Leu Ile Met Val Cys Tyr Leu Met Ala Val Gly Asn Cys Leu Leu 

                85                  90                  95      

Phe Ala Phe Asn Arg Asp Tyr Leu Thr Leu Ile Thr Ala Gly Val Leu 

            100                 105                 110         

Leu Ala Ser Val Ala Asn Thr Ala Met Pro Gln Ile Phe Ala Leu Ala 

        115                 120                 125             

Arg Glu Tyr Ala Asp Ser Ser Ala Arg Glu Val Val Met Phe Ser Ser 

    130                 135                 140                 

Ile Met Arg Ala Gln Leu Ser Leu Ala Trp Val Ile Gly Pro Pro Leu 

145                 150                 155                 160 

Ser Phe Met Leu Ala Leu Asn Tyr Gly Phe Thr Leu Met Phe Ser Ile 

                165                 170                 175     

Ala Ala Gly Ile Phe Val Leu Ser Ala Leu Val Val Trp Phe Ile Leu 

            180                 185                 190         

Pro Ser Val Pro Arg Ala Glu Pro Val Val Asp Ala Pro Val Val Val 

        195                 200                 205             

Gln Gly Ser Leu Phe Ala Asp Lys Asn Val Leu Leu Leu Phe Ile Ala 

    210                 215                 220                 

Ser Met Leu Met Trp Thr Cys Asn Thr Met Tyr Ile Ile Asp Met Pro 

225                 230                 235                 240 

Leu Tyr Ile Thr Ala Ser Leu Gly Leu Pro Glu Arg Leu Ala Gly Leu 

                245                 250                 255     

Leu Met Gly Thr Ala Ala Gly Leu Glu Ile Pro Ile Met Leu Leu Ala 

            260                 265                 270         

Gly Tyr Ser Val Arg Tyr Phe Gly Lys Arg Lys Ile Met Leu Phe Ala 

        275                 280                 285             

Val Leu Ala Gly Val Leu Phe Tyr Thr Gly Leu Val Leu Phe Lys Phe 

    290                 295                 300                 

Lys Thr Ala Leu Met Leu Leu Gln Ile Phe Asn Ala Ile Phe Ile Gly 

305                 310                 315                 320 

Ile Val Ala Gly Ile Gly Met Leu Tyr Phe Gln Asp Leu Met Pro Gly 

                325                 330                 335     

Arg Ala Gly Ala Ala Thr Thr Leu Phe Thr Asn Ser Ile Ser Thr Gly 

            340                 345                 350         

Val Ile Leu Ala Gly Val Leu Gln Gly Gly Leu Thr Glu Thr Trp Gly 

        355                 360                 365             

His Asp Ser Val Tyr Val Met Ala Met Val Leu Ser Ile Leu Ala Leu 

    370                 375                 380                 

Ile Ile Cys Ala Arg Val Arg Glu Ala 

385                 390             



<210> 68

<211> 107

<212> DNA

<213> Escherichia coli





<220> 

<223> Plac, lac operon promoter



<400> 68

tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat     60



gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagct                  107





<210> 69

<211> 291

<212> DNA

<213> Artificial Sequence





<220> 

<223> PgatY_70UTR, variant of E. coli promoter for gatYZABCD;

      tagatose-1,6-bisP aldolase



<300> 

<310> WO/2020/255054

<311> 19.06.2020

<312> 24.12.2020



<400> 69

cggcaaccta tgcctgatgc gacgctgaag cgtcttatca tgcctacata gcactgccac     60



gtatgtttac accgcatccg gcataaaaac acgcgcactt tgctacggct tccctatcgg    120



gaggccgttt ttttgccttt cactcctcga ataattttca tattgtcgtt tttgtgatcg    180



ttatctcgat atttaaaaac aaataatttc attatatttt gtgcctacaa gcatcgtgga    240



ggtccgtgac tttcacgcat acaacaaaca ttaaccaagg aggaaacagc t             291





<210> 70

<211> 300

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpF, E. Coli promoter sequence of glpFKX operon



<300> 

<310> WO/2019/123324

<311> 19.12.2018

<312> 27.06.2019



<400> 70

gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60



ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120



tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180



tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240



catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagga ggaaacagct    300





<210> 71

<211> 300

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpF_SD1, variant of PglpF E. Coli promoter sequence of glpFKX

      operon



<300> 

<310> WO/2019/123324

<311> 19.12.2018

<312> 27.06.2019



<400> 71

gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60



ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120



tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180



tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240



catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaaatt cgaaacagct    300





<210> 72

<211> 300

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpF_SD10, variant of PglpF E. Coli promoter sequence of glpFKX

      operon



<300> 

<310> WO/2019/123324

<311> 19.12.2018

<312> 27.06.2019



<400> 72

gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60



ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120



tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180



tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240



catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaactg agaaacagct    300





<210> 73

<211> 300

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpF_SD2, variant of PglpF E. Coli promoter sequence of glpFKX

      operon



<300> 

<310> WO/2019/123324

<311> 19.12.2018

<312> 27.06.2019



<400> 73

gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60



ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120



tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180



tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240



catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagcg caaaacagct    300





<210> 74

<211> 300

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpF_SD3 variant of PglpF E. Coli promoter sequence of glpFKX

      operon



<300> 

<310> WO/2019/123324

<311> 19.12.2018

<312> 27.06.2019



<400> 74

gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60



ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120



tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180



tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240



catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagaa caaaacagct    300





<210> 75

<211> 300

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpF_SD4, variant of PglpF E. Coli promoter sequence of glpFKX

      operon



<300> 

<310> WO/2019/123324

<311> 19.12.2018

<312> 27.06.2019



<400> 75

gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60



ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120



tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180



tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240



catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaacta ggaaacagct    300





<210> 76

<211> 300

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpF_SD5, variant of PglpF E. Coli promoter sequence of glpFKX

      operon



<300> 

<310> WO/2019/123324

<311> 19.12.2018

<312> 27.06.2019



<400> 76

gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60



ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120



tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180



tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240



catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaaccg agaaacagct    300





<210> 77

<211> 300

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpF_SD6, variant of PglpF E. Coli promoter sequence of glpFKX

      operon



<300> 

<310> WO/2019/123324

<311> 19.12.2018

<312> 27.06.2019



<400> 77

gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60



ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120



tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180



tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240



catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagag ctaaacagct    300





<210> 78

<211> 300

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpF_SD7, variant of PglpF E. Coli promoter sequence of glpFKX

      operon



<300> 

<310> WO/2019/123324

<311> 19.12.2018

<312> 27.06.2019



<400> 78

gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60



ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120



tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180



tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240



catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagag caaaacagct    300





<210> 79

<211> 300

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpF_SD8, variant of PglpF E. Coli promoter sequence of glpFKX

      operon



<300> 

<310> WO/2019/123324

<311> 19.12.2018

<312> 27.06.2019



<400> 79

gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60



ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120



tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180



tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240



catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagag aaaaacagct    300





<210> 80

<211> 300

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpF_SD9, variant of PglpF E. Coli promoter sequence of glpFKX

      operon



<300> 

<310> WO/2019/123324

<311> 19.12.2018

<312> 27.06.2019



<400> 80

gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60



ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120



tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180



tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240



catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaaagg aaaaacagct    300





<210> 81

<211> 107

<212> DNA

<213> Artificial Sequence





<220> 

<223> Plac_16UTR, variant of E. coli lac operon promoter



<300> 

<310> WO/2019/123324

<311> 19.12.2018

<312> 27.06.2019



<400> 81

tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat     60



gttgtgtgga attgtgagcg gataacaatt tcaaggagga aacagct                  107





<210> 82

<211> 203

<212> DNA

<213> Artificial Sequence





<220> 

<223> PmglB_70UTR, variant of E. coli promoter for mglBAC

      galactose/methyl-galactosidade transporter



<300> 

<310> WO/2020/255054

<311> 19.06.2020

<312> 24.12.2020



<400> 82

tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60



aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120



tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180



cattaaccaa ggaggaaaca gct                                            203





<210> 83

<211> 203

<212> DNA

<213> Artificial Sequence





<220> 

<223> PmglB_70UTR_SD4, variant of E. coli promoter for mglBAC;

      galactose/methyl-galactosidade transporter



<300> 

<310> WO/2020/255054

<311> 19.06.2020

<312> 24.12.2020



<400> 83

tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60



aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120



tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180



cattaaccaa ctaggaaaca gct                                            203





<210> 84

<211> 76

<212> DNA

<213> Artificial Sequence





<220> 

<223> CP6 promotor sequence



<400> 84

catgtgggag tttattcttg acacagatat ttccggatga tataataact gagtactgtt     60



cacacaggaa acagct                                                     76





<210> 85

<211> 269

<212> DNA

<213> Escherichia coli





<220> 

<223> PosmY, promotor for osmY-ytjA; perplasmic chaperone



<400> 85

caaaattgtg atctatattt aacaaagtga tgacatttct gacggcgtta aataccgttc     60



aatgcgtaga tatcagtatc taaagccgtc gattgtcatt ctaccgatat taataactga    120



ttcagaggct gtaatggtcg ttattcatca ctcatcgctt ttgtgatggc gaccattgac    180



ttctgtagag ggtgaagtct ctccctattc agcaatgcaa cctcgtgttg ccaggctcaa    240



attacgagca aaccaaggag gaaacagct                                      269





<210> 86

<211> 483

<212> PRT

<213> Avibacterium gallinarum





<220> 

<223> SacC_Agal, glycoside hydrolase family 32 protein, WP_103853210.1



<400> 86

Met Ile Ile Phe Asn Glu Gly Lys Tyr Lys Ser Leu Tyr Ala Ala Glu 

1               5                   10                  15      

Gln Gly Glu Leu Glu Lys Ile Ala Gln Thr Val Ala Gln Asp Gln Asp 

            20                  25                  30          

Phe Arg Pro Val Tyr His Leu Ala Pro Pro Thr Gly Leu Leu Asn Asp 

        35                  40                  45              

Pro Asn Gly Leu Ile Phe Asp Gly Glu Lys Tyr His Leu Phe Tyr Gln 

    50                  55                  60                  

Trp Tyr Pro Phe Asp Ala Leu His Gly Met Lys His Trp Gln His Phe 

65                  70                  75                  80  

Ile Thr Gln Asp Phe Lys Gln Phe Ser Gln Ala Asp Leu Leu Val Pro 

                85                  90                  95      

Cys Glu Leu Tyr Glu Ser His Gly Cys Tyr Ser Gly Gly Ala Val Lys 

            100                 105                 110         

Ile Gly Asp Gln Ile Ala Val Phe Tyr Thr Gly Asn Thr Arg Arg Pro 

        115                 120                 125             

Ser Asp Asn Gln Arg Val Pro Tyr Gln Asn Leu Ala Ile Phe Ser Lys 

    130                 135                 140                 

Asp Gly Lys Leu Leu Ser Lys Arg Pro Leu Ile Glu Gln Ala Pro Gln 

145                 150                 155                 160 

Gly Tyr Thr Glu His Val Arg Asp Pro Lys Pro Phe Leu Thr Lys Asp 

                165                 170                 175     

Gly Lys Ile Arg Phe Ile Cys Gly Ala Gln Arg Glu Asn Leu Thr Gly 

            180                 185                 190         

Thr Ala Leu Val Phe Glu Met Asp Asn Leu Ala Asp Thr Pro Arg Leu 

        195                 200                 205             

Leu Gly Glu Leu Ala Leu Pro Ala Phe Asp Asn Gln Gly Val Phe Met 

    210                 215                 220                 

Trp Glu Cys Pro Asp Leu Ser Gln Met Gly Asp Lys Ser Leu Phe Ile 

225                 230                 235                 240 

Trp Ser Pro Gln Gly Lys Ala Arg Glu Leu Glu Gln Tyr Gln Asn Asn 

                245                 250                 255     

Tyr His Ala Val Tyr Ala Leu Gly Glu Leu Ala Asp Arg Gln Phe His 

            260                 265                 270         

Ala Glu Gln Ile Ala Glu Leu Asp Gln Gly Phe Asp Phe Tyr Ala Pro 

        275                 280                 285             

Gln Thr Phe Ser Gly Thr Gln Thr Met Leu Leu Gly Trp Val Gly Leu 

    290                 295                 300                 

Pro Asp Leu Ser Tyr Pro Thr Asp Leu Tyr Lys Trp His Ser Met Leu 

305                 310                 315                 320 

Ser Met Pro Arg Gln Leu Arg Leu Gln Asp Gly Lys Ile Tyr Gln Gln 

                325                 330                 335     

Pro Ile Glu Asn Ile Tyr Lys Asn Leu Thr Ala Leu Gln Ser Ile Thr 

            340                 345                 350         

Val Glu Lys Glu Ala Glu Ile Ala Asp Leu Asp Arg Ala Tyr Leu Lys 

        355                 360                 365             

Phe Asp Ala Asn Ala Gln Pro Phe Ser Leu Lys Phe Phe Asn Asn Ala 

    370                 375                 380                 

Gln Asn Gln Arg Leu Ile Leu Ser Tyr Asp Gly Glu Met Leu Cys Leu 

385                 390                 395                 400 

Asp Arg Ser Gln Thr Glu Gln Thr Asp Ser Met Lys Ser Phe Gly Asp 

                405                 410                 415     

Lys Arg Tyr Cys Arg Ile Glu Asp Leu Arg Gln Val Glu Ile Phe Phe 

            420                 425                 430         

Asp Arg Ser Val Ala Glu Ile Phe Leu Asn Gln Gly Glu Lys Ala Met 

        435                 440                 445             

Thr Ser Arg Phe Phe Ile Cys Ala Arg Glu Asn Gln Leu Cys Thr Asp 

    450                 455                 460                 

Lys Pro Leu Thr Leu Gln Val Gly Tyr Pro Lys Lys Ile Glu Val Asp 

465                 470                 475                 480 

Tyr Thr Lys 

            



<210> 87

<211> 548

<212> PRT

<213> Arthrobacter globiformis





<220> 

<223> Bff, beta-fructofuranosidase protein, BAD18121.1



<400> 87

Met Glu Arg Thr Cys Ile Thr Val Arg Ala Ile Val Arg Phe His Ile 

1               5                   10                  15      

Glu Gln Arg Gln Thr Ile Val Asn Lys Gln Arg Thr Lys Arg Gly Ile 

            20                  25                  30          

Leu Thr Ala Ala Leu Ser Ile Gly Ala Leu Gly Ala Thr Leu Ile Ser 

        35                  40                  45              

Gly Pro Ala Val Ala Ala Thr Asp Ala Ala Pro Gly Phe Pro Gln Pro 

    50                  55                  60                  

Thr Glu His Thr Gln Lys Ala Tyr Ser Pro Thr Asp Asn Phe Thr Ser 

65                  70                  75                  80  

Arg Trp Thr Arg Ala Asp Ala Lys Gln Leu Lys Ala Met Ser Asp Pro 

                85                  90                  95      

Asp Ala Gly Ser Arg Glu Asn Ser Met Pro Thr Glu Tyr Thr Met Pro 

            100                 105                 110         

Thr Val Ser Gln Asp Phe Pro Asp Met Ser Asn Glu Lys Val Trp Val 

        115                 120                 125             

Trp Asp Thr Trp Pro Leu Ile Asp Glu Asn Ala Asn Gln Tyr Ser Val 

    130                 135                 140                 

Asn Gly Gln Glu Ile Ile Phe Ser Leu Val Ala Asp Arg Lys Leu Gly 

145                 150                 155                 160 

Phe Asp Glu Arg His Gln Tyr Ala Arg Ile Gly Tyr Phe Tyr Arg Pro 

                165                 170                 175     

Ala Gly Ile Pro Ala Asp Glu Arg Pro Glu Asp Gly Gly Trp Thr Tyr 

            180                 185                 190         

Gly Gly Gln Val Phe Asp Glu Gly Val Thr Gly Lys Ile Phe Glu Asp 

        195                 200                 205             

Gln Ser Phe Thr His Gln Thr Gln Trp Ser Gly Ser Ala Arg Val Ser 

    210                 215                 220                 

Lys Asn Gly Glu Ile Lys Leu Phe Phe Thr Asp Val Ala Phe Tyr Arg 

225                 230                 235                 240 

Asp Lys Asp Gly Gln Asp Val Lys Pro Tyr Asp Ser Arg Ile Ala Leu 

                245                 250                 255     

Ser Val Gly His Val His Ser Asn Lys Lys Gly Val Lys Leu Thr Gly 

            260                 265                 270         

Phe Asn Lys Val Lys Glu Leu Leu Gln Ala Asp Gly Lys Asn Tyr Gln 

        275                 280                 285             

Asn Ala Ala Gln Asn Ser Tyr Tyr Asn Phe Arg Asp Pro Phe Thr Phe 

    290                 295                 300                 

Val Asp Pro Ala His Pro Gly Glu Thr Tyr Met Val Phe Glu Gly Asn 

305                 310                 315                 320 

Ser Ala Met Asp Arg Asp Glu Ala Lys Cys Thr Ala Glu Asp Leu Gly 

                325                 330                 335     

Tyr Arg Glu Gly Glu Thr Asn Gly Glu Thr Val Glu Gln Val Asn Asn 

            340                 345                 350         

Ser Gly Ala Thr Tyr Gln Ile Gly Asn Val Gly Leu Ala Arg Ala Lys 

        355                 360                 365             

Asn Lys Ala Leu Thr Glu Trp Glu Phe Leu Pro Pro Ile Leu Ser Ala 

    370                 375                 380                 

Asn Cys Val Thr Asp Gln Thr Glu Arg Pro Gln Ile Tyr Met Gln Asp 

385                 390                 395                 400 

Gly Lys Tyr Tyr Leu Phe Thr Ile Ser His Arg Ser Thr Phe Ala Thr 

                405                 410                 415     

Gly Ile Asp Gly Pro Glu Gly Val Tyr Gly Phe Val Gly Asn Gly Ile 

            420                 425                 430         

Arg Ser Asp Tyr Gln Pro Leu Asn Arg Gly Ser Gly Leu Ala Leu Gly 

        435                 440                 445             

Ser Pro Thr Asn Leu Asn Phe Ala Ala Gly Thr Pro Phe Ala Pro Asp 

    450                 455                 460                 

Tyr Asn Gln His Pro Gly Gln Phe Gln Ala Tyr Ser His Tyr Val Met 

465                 470                 475                 480 

Pro Gly Gly Leu Val Gln Ser Phe Ile Asp Thr Ile Gly Thr Lys Asp 

                485                 490                 495     

Asn Phe Val Arg Gly Gly Thr Leu Gly Pro Thr Val Lys Leu Asn Ile 

            500                 505                 510         

Lys Gly Asp Ser Ala Thr Val Asp Tyr Asn Tyr Gly Asp Asn Gly Leu 

        515                 520                 525             

Gly Gly Trp Ala Asp Ile Pro Ala Asn Arg Glu Leu Lys Asn Ser Lys 

    530                 535                 540                 

Ala Val Ala Lys 

545             



<210> 88

<211> 505

<212> PRT

<213> Klebsiella pneumoniae





<220> 

<223> ScrY, sucrose porin, GeneBank ID CAA40657.1



<300> 

<310> WO/2015/197082

<311> 29.06.2015

<312> 30.12.2015



<400> 88

Met Tyr Lys Lys Arg Lys Leu Ala Ile Leu Ile Ala Leu Leu Thr Gly 

1               5                   10                  15      

Thr Ala Ala Ala His Gly Gln Thr Asp Leu Asn Ser Ile Glu Ala Arg 

            20                  25                  30          

Leu Ala Ala Leu Glu Lys Arg Leu Gln Asp Ala Glu Thr Arg Ala Ser 

        35                  40                  45              

Thr Ala Glu Ser Arg Ala Ala Ser Ala Glu Gln Lys Val Gln Gln Leu 

    50                  55                  60                  

Thr Gln Gln Gln Gln Gln Thr Gln Ala Thr Thr Gln Gln Val Ala Arg 

65                  70                  75                  80  

Arg Thr Thr Gln Leu Glu Glu Lys Ala Glu Arg Pro Gly Gly Phe Glu 

                85                  90                  95      

Phe His Gly Tyr Ala Arg Ser Gly Val Ile Met Asn Asp Ser Ala Ala 

            100                 105                 110         

Ser Thr Lys Ser Gly Ala Tyr Met Thr Pro Ala Gly Glu Thr Gly Gly 

        115                 120                 125             

Ala Ile Gly Arg Leu Gly Asn Gln Ala Asp Thr Tyr Val Glu Met Asn 

    130                 135                 140                 

Leu Glu His Lys Gln Thr Leu Asp Asn Gly Ala Thr Thr Arg Phe Lys 

145                 150                 155                 160 

Val Met Val Ala Asp Gly Gln Thr Thr Tyr Asn Asp Trp Thr Ala Ser 

                165                 170                 175     

Ser Ser Asp Leu Asn Val Arg Gln Ala Phe Val Glu Leu Gly Asn Leu 

            180                 185                 190         

Pro Thr Phe Glu Gly Pro Phe Lys Gly Ser Thr Leu Trp Ala Gly Lys 

        195                 200                 205             

Arg Phe Asp Arg Asp Asn Phe Asp Ile His Trp Ile Asp Ser Asp Val 

    210                 215                 220                 

Val Phe Leu Ala Gly Thr Gly Gly Gly Ile Tyr Asp Val Lys Trp Asn 

225                 230                 235                 240 

Asp Ser Leu Arg Ser Asn Phe Ser Leu Tyr Gly Arg Asn Phe Gly Asp 

                245                 250                 255     

Ile Ala Asp Ser Ser Asn Ser Val Gln Asn Tyr Ile Val Ser Met Asn 

            260                 265                 270         

Asn Phe Ala Gly Pro Val Gln Met Met Val Ser Gly Met Arg Ala Lys 

        275                 280                 285             

Asp Asn Asp Asp Arg Gln Asp Ala Asn Gly Asn Leu Val Lys Gly Asp 

    290                 295                 300                 

Ala Ala Asn Thr Gly Val His Ala Leu Leu Gly Leu His Asn Glu Ser 

305                 310                 315                 320 

Phe Tyr Gly Leu Arg Asp Gly Thr Ser Lys Thr Ala Leu Leu Tyr Gly 

                325                 330                 335     

His Gly Leu Gly Ala Glu Val Lys Gly Ile Gly Ser Asp Gly Ala Leu 

            340                 345                 350         

Arg Pro Gly Ala Asn Thr Trp Arg Phe Ala Ser Tyr Gly Thr Thr Pro 

        355                 360                 365             

Leu Ser Asp Arg Trp Phe Ile Ala Pro Ala Val Leu Ala Gln Ser Ser 

    370                 375                 380                 

Lys Asp Arg Tyr Val Asp Gly Asp Ser Tyr Gln Trp Ala Thr Leu Asn 

385                 390                 395                 400 

Leu Arg Leu Ile Gln Glu Val Thr Gln Asn Phe Ala Leu Ala Trp Glu 

                405                 410                 415     

Gly Ser Tyr Gln Tyr Met Asp Leu Gln Pro Glu Gly Tyr Asn Asp Arg 

            420                 425                 430         

His Ala Val Asn Gly Ser Phe Tyr Lys Leu Thr Phe Ala Pro Thr Phe 

        435                 440                 445             

Lys Val Gly Ser Ile Gly Asp Phe Phe Ser Arg Pro Glu Ile Arg Phe 

    450                 455                 460                 

Tyr Thr Ser Trp Met Asp Trp Ser Lys Lys Leu Asp Asn Tyr Ala Asn 

465                 470                 475                 480 

Asp Asp Ala Leu Gly Ser Asn Gly Phe Lys Ser Gly Gly Glu Trp Ser 

                485                 490                 495     

Phe Gly Met Gln Met Glu Thr Trp Phe 

            500                 505 



<210> 89

<211> 456

<212> PRT

<213> Klebsiella pneumoniae





<220> 

<223> ScrA, sucrose-specific enzyme II, GeneBank ID: CAA40658.1



<300> 

<310> WO/2015/197082

<311> 29.06.2015

<312> 30.12.2015



<400> 89

Met Asp Phe Glu Gln Ile Ser Arg Ser Leu Leu Pro Leu Leu Gly Gly 

1               5                   10                  15      

Lys Glu Asn Ile Ala Ser Ala Ala His Cys Ala Thr Arg Leu Arg Leu 

            20                  25                  30          

Val Leu Val Asp Asp Ala Leu Ala Asp Gln Gln Ala Ile Gly Lys Ile 

        35                  40                  45              

Asp Gly Val Lys Gly Cys Phe Arg Asn Ala Gly Gln Met Gln Ile Ile 

    50                  55                  60                  

Phe Gly Thr Gly Val Val Asn Lys Val Tyr Ala Ala Phe Ile Gln Ala 

65                  70                  75                  80  

Ala Gly Ile Ser Glu Ser Ser Lys Ser Glu Ala Ala Asp Leu Ala Ala 

                85                  90                  95      

Lys Lys Leu Asn Pro Phe Gln Arg Ile Ala Arg Leu Leu Ser Asn Ile 

            100                 105                 110         

Phe Val Pro Ile Ile Pro Ala Ile Val Ala Ser Gly Leu Leu Met Gly 

        115                 120                 125             

Leu Leu Gly Met Val Lys Thr Tyr Gly Trp Val Asp Pro Ser Asn Ala 

    130                 135                 140                 

Leu Tyr Ile Met Leu Asp Met Cys Ser Ser Ala Ala Phe Ile Ile Leu 

145                 150                 155                 160 

Pro Ile Leu Ile Gly Phe Thr Ala Ala Arg Glu Phe Gly Gly Asn Pro 

                165                 170                 175     

Tyr Leu Gly Ala Thr Leu Gly Gly Ile Leu Thr His Pro Ala Leu Thr 

            180                 185                 190         

Asn Ala Trp Gly Val Ala Ala Gly Phe His Thr Met Asn Phe Phe Gly 

        195                 200                 205             

Ile Glu Val Ala Met Ile Gly Tyr Gln Gly Thr Val Phe Pro Val Leu 

    210                 215                 220                 

Leu Ala Val Trp Phe Met Ser Met Val Glu Lys Arg Leu Arg Arg Val 

225                 230                 235                 240 

Ile Pro Asp Ala Leu Asp Leu Ile Leu Thr Pro Phe Leu Thr Val Ile 

                245                 250                 255     

Ile Ser Gly Phe Ile Ala Leu Leu Leu Ile Gly Pro Ala Gly Arg Ala 

            260                 265                 270         

Leu Gly Asp Gly Ile Ser Phe Ile Leu Ser Thr Leu Ile Ser His Ala 

        275                 280                 285             

Gly Trp Leu Ala Gly Leu Leu Phe Gly Gly Leu Tyr Ser Val Ile Val 

    290                 295                 300                 

Ile Thr Gly Ile His His Ser Phe His Ala Ile Glu Ala Gly Leu Leu 

305                 310                 315                 320 

Gly Asn Pro Ser Ile Gly Val Asn Phe Leu Leu Pro Ile Trp Ala Met 

                325                 330                 335     

Ala Asn Val Ala Gln Gly Gly Ala Cys Phe Ala Val Trp Phe Lys Thr 

            340                 345                 350         

Lys Asp Ala Lys Ile Lys Ala Ile Thr Leu Pro Ser Ala Phe Ser Ala 

        355                 360                 365             

Met Leu Gly Ile Thr Glu Ala Ala Ile Phe Gly Ile Asn Leu Arg Phe 

    370                 375                 380                 

Val Lys Pro Phe Ile Ala Ala Leu Val Gly Gly Ala Ala Gly Gly Ala 

385                 390                 395                 400 

Trp Val Val Ser Met His Val Tyr Met Thr Ala Val Gly Leu Thr Ala 

                405                 410                 415     

Ile Pro Gly Met Ala Ile Val Gln Ala Ser Ser Leu Leu Asn Tyr Ile 

            420                 425                 430         

Ile Gly Met Ala Ile Ala Phe Ala Val Ala Phe Ala Leu Ser Leu Thr 

        435                 440                 445             

Leu Lys Tyr Lys Thr Asp Ala Glu 

    450                 455     



<210> 90

<211> 466

<212> PRT

<213> Salmonella enterica subsp. enterica serovar Typhimurium





<220> 

<223> ScrB, beta-fructofuranosidase, GeneBank ID: CAA47974.1



<300> 

<310> WO/2015/197082

<311> 29.06.2015

<312> 30.12.2015



<400> 90

Met Ser Leu Pro Ser Arg Leu Pro Ala Ile Leu Gln Ala Val Met Gln 

1               5                   10                  15      

Gly Gln Pro Arg Ala Leu Ala Asp Ser His Tyr Pro Arg Trp His His 

            20                  25                  30          

Ala Pro Val Thr Gly Leu Met Asn Asp Pro Asn Gly Phe Ile Glu Phe 

        35                  40                  45              

Ala Gly Arg Tyr His Leu Phe Tyr Gln Trp Asn Pro Leu Ala Cys Asp 

    50                  55                  60                  

His Thr Phe Lys Cys Trp Ala His Trp Ser Ser Ile Asp Leu Leu His 

65                  70                  75                  80  

Trp Gln His Glu Pro Ile Ala Leu Met Pro Asp Glu Glu Tyr Asp Arg 

                85                  90                  95      

Asn Gly Cys Tyr Ser Gly Ser Ala Val Asp Asn Asn Gly Thr Leu Thr 

            100                 105                 110         

Leu Cys Tyr Thr Gly Asn Val Lys Phe Ala Glu Gly Gly Arg Thr Ala 

        115                 120                 125             

Trp Gln Cys Leu Ala Thr Glu Asn Ala Asp Gly Thr Phe Arg Lys Ile 

    130                 135                 140                 

Gly Pro Val Leu Pro Leu Pro Glu Gly Tyr Thr Gly His Val Arg Asp 

145                 150                 155                 160 

Pro Lys Val Trp Arg His Glu Asp Leu Trp Tyr Met Val Leu Gly Ala 

                165                 170                 175     

Gln Asp Arg Gln Lys Arg Gly Lys Val Leu Leu Phe Ser Ser Ala Asp 

            180                 185                 190         

Leu His Gln Trp Thr Ser Met Gly Glu Ile Ala Gly His Gly Ile Asn 

        195                 200                 205             

Gly Leu Asp Asp Val Gly Tyr Met Trp Glu Cys Pro Asp Leu Phe Pro 

    210                 215                 220                 

Leu Gly Asp Gln His Ile Leu Ile Cys Cys Pro Gln Gly Ile Ala Arg 

225                 230                 235                 240 

Glu Glu Glu Cys Tyr Leu Asn Thr Tyr Pro Ala Val Trp Met Ala Gly 

                245                 250                 255     

Glu Phe Asp Tyr Ala Ala Gly Ala Phe Arg His Gly Glu Leu His Glu 

            260                 265                 270         

Leu Asp Ala Gly Phe Glu Phe Tyr Ala Pro Gln Thr Met Leu Thr Ser 

        275                 280                 285             

Asp Gly Arg Arg Leu Leu Val Gly Trp Met Gly Val Pro Glu Gly Glu 

    290                 295                 300                 

Glu Met Leu Gln Pro Thr Leu Asn Asn Gly Trp Ile His Gln Met Thr 

305                 310                 315                 320 

Cys Leu Arg Glu Leu Glu Phe Ile Asn Gly Gln Leu Tyr Gln Arg Pro 

                325                 330                 335     

Leu Arg Glu Leu Ser Ala Leu Arg Gly Glu Ala Asn Gly Trp Ser Gly 

            340                 345                 350         

Asn Ala Leu Pro Leu Ala Pro Met Glu Ile Asp Leu Gln Thr Arg Gly 

        355                 360                 365             

Gly Asp Met Leu Ser Leu Asp Phe Gly Gly Val Leu Thr Leu Glu Cys 

    370                 375                 380                 

Asp Ala Ser Gly Leu Arg Leu Ala Arg Arg Ser Leu Ala Ser Asp Glu 

385                 390                 395                 400 

Met His Tyr Arg Tyr Trp Arg Gly Asn Val Arg Ser Leu Arg Val Phe 

                405                 410                 415     

Ile Asp Gln Ser Ser Val Glu Ile Phe Ile Asn Gly Gly Glu Gly Val 

            420                 425                 430         

Met Ser Ser Arg Tyr Phe Pro Ala Cys Ser Gly Gln Leu Thr Phe Ser 

        435                 440                 445             

Gly Ile Thr Pro Asp Ala Phe Cys Tyr Trp Pro Leu Arg Thr Cys Met 

    450                 455                 460                 

Val Glu 

465     



<210> 91

<211> 334

<212> PRT

<213> Salmonella enterica subsp. enterica serovar Typhimurium





<220> 

<223> ScrR, sucrose repressor, GeneBank ID: CAA47975.1



<300> 

<310> WO/2015/197082

<311> 29.06.2015

<312> 30.12.2015



<400> 91

Met Lys Thr Lys Arg Val Thr Ile Lys Asp Ile Ala Glu Gln Ala Gly 

1               5                   10                  15      

Val Ser Lys Ala Thr Ala Ser Leu Val Leu Asn Gly Arg Gly Lys Glu 

            20                  25                  30          

Leu Arg Val Ala Gln Glu Thr Arg Glu Arg Val Leu Ser Ile Ala Arg 

        35                  40                  45              

Lys His His Tyr Gln Pro Ser Ile His Ala Arg Ser Leu Arg Asn Asn 

    50                  55                  60                  

Arg Ser His Thr Ile Gly Leu Val Val Pro Glu Ile Thr Asn His Gly 

65                  70                  75                  80  

Phe Ala Val Phe Ala His Glu Leu Glu Met Leu Cys Arg Glu Ala Gly 

                85                  90                  95      

Val Gln Leu Leu Ile Ser Cys Thr Asp Glu Asn Pro Gly Gln Glu Ser 

            100                 105                 110         

Val Val Val Asn Asn Met Ile Ala Arg Gln Val Asp Gly Met Ile Val 

        115                 120                 125             

Ala Ser Cys Met His Asn Asp Ala Asp Tyr Leu Lys Leu Ser Gln Gln 

    130                 135                 140                 

Leu Pro Val Val Leu Phe Asp Arg Cys Pro Asn Glu Ser Ala Leu Pro 

145                 150                 155                 160 

Leu Val Met Thr Asp Ser Ile Thr Pro Thr Ala Glu Leu Ile Ser Arg 

                165                 170                 175     

Ile Ala Pro Gln His Ser Asp Glu Phe Trp Phe Leu Gly Gly Gln Ala 

            180                 185                 190         

Arg Leu Ser Pro Ser Arg Asp Arg Leu Thr Gly Phe Thr Gln Gly Leu 

        195                 200                 205             

Ala Gln Ala Gly Ile Ala Leu Arg Pro Glu Trp Val Ile Asn Gly Asn 

    210                 215                 220                 

Tyr His Pro Ser Ser Gly Tyr Glu Met Phe Ala Ala Leu Cys Ala Arg 

225                 230                 235                 240 

Leu Gly Arg Pro Pro Lys Ala Leu Phe Thr Ala Ala Cys Gly Leu Leu 

                245                 250                 255     

Glu Gly Val Leu Arg Tyr Met Ser Gln His His Leu Leu Asp Ser Asp 

            260                 265                 270         

Ile His Leu Thr Ser Phe Asp Asp His Tyr Leu Tyr Asp Ser Leu Ser 

        275                 280                 285             

Leu Arg Ile Asp Thr Val Gln Gln Asp Asn Arg Gln Leu Ala Trp His 

    290                 295                 300                 

Cys Tyr Asp Leu Ile Ser Gln Leu Ile Glu Gly Asp Thr Pro Glu Thr 

305                 310                 315                 320 

Leu Gln Arg Tyr Leu Pro Ala Thr Leu Gln Phe Arg His Gln 

                325                 330                 



<210> 92

<211> 730

<212> DNA

<213> Escherichia coli





<220> 

<223> glpR coding sequence



<400> 92

atgaaacaaa cacaacgtca caacggtatt atcgaactgg ttaaacagca gggttatgtc      60



agtaccgaag agctggtaga gcatttctcc gtcagcccgc agactattcg ccgcgacctc     120



aatgagctgg cggagcaaaa cctgatcctg gccatcatgg cggtgcggcg ctgccttcca     180



gttcggttaa cacgccgtgg cacgatcgca aggccaccca gaccgaagaa aaagagcgca     240



tcgcccgcaa agtggcggag caaatcccca atggctcgac gctgtttatc gatatcggca     300



ccacgccgga agcggtagcg cacgcactgc tcaatcacag caatttgcgc attgtcacca     360



acaatctcaa cgttgctaac acgttgatgg taaaagaaga ttttcgcatc attctcgccg     420



gtggcgaatt acgcagccgc gatggcggga tcattggcga agcgacgctc gattttatct     480



cccagttccg ccttgatttc ggcattctgg ggataagcgg catcgatagc gacggctcgc     540



tgctggagtt cgattaccac gaagttcgca ccaaacgcgc cattattgag aactcgcgcc     600



acgttatgct ggttgtcgat cactcgaaat ttggccgtaa cgcgatggtc aatatgggca     660



gcatcagcat ggtagatgcc gtctacaccg acgccccgcc gccagtaagc gtgatgcagg     720



tgctgacgga                                                            730





<210> 93

<211> 203

<212> DNA

<213> Artificial Sequence





<220> 

<223> PmglB_70UTR_SD8 promoter sequence



<400> 93

tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60



aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120



tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180



cattaaccaa gagaaaaaca gct                                            203





<210> 94

<211> 203

<212> DNA

<213> Artificial Sequence





<220> 

<223> PmglB_70UTR_SD10 promoter sequence



<400> 94

tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60



aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120



tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180



cattaaccaa ctgagaaaca gct                                            203





<210> 95

<211> 203

<212> DNA

<213> Artificial Sequence





<220> 

<223> PmglB-54UTR promoter sequence



<400> 95

tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60



aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120



tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180



cattaacaaa aaccggagat acc                                            203





<210> 96

<211> 141

<212> DNA

<213> Artificial Sequence





<220> 

<223> Plac 70_UTR promoter sequence



<400> 96

tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat     60



gttgtgtgga atgcctacaa gcatcgtgga ggtccgtgac tttcacgcat acaacaaaca    120



ttaaccaagg aggaaacagc t                                              141





<210> 97

<211> 203

<212> DNA

<213> Artificial Sequence





<220> 

<223> PmglB_70UTR_SD9 promoter sequence



<400> 97

tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60



aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120



tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180



cattaaccaa aggaaaaaca gct                                            203





<210> 98

<211> 203

<212> DNA

<213> Artificial Sequence





<220> 

<223> PmglB_70UTR_SD5 promoter sequence



<400> 98

tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60



aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120



tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180



cattaaccaa ccgagaaaca gct                                            203





<210> 99

<211> 203

<212> DNA

<213> Artificial Sequence





<220> 

<223> PmglB_70UTR_SD7 promoter sequence



<400> 99

tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60



aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120



tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180



cattaaccaa gagcaaaaca gct                                            203





<210> 100

<211> 189

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpA_70UTR promoter sequence



<400> 100

gaaaacattc ataaattaaa tgtgaattgc cgcacacatt attaaataag atttacaaaa     60



tgttcaaaat gacgcatgaa atcacgtttc actttcgaat tatgagcgaa tatgcgcgat    120



gcctacaagc atcgtggagg tccgtgactt tcacgcatac aacaaacatt aaccaaggag    180



gaaacagct                                                            189





<210> 101

<211> 239

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpT_70UTR promoter sequence



<400> 101

ccatttagcc atagtaaaaa catgaattgt ttgatttcgc gcatattcgc tcataattcg     60



aaagtgaaac gtgatttcat gcgtcatttt gaacattttg taaatcttat ttaataatgt    120



gtgcggcaat tcacatttaa tttatgaatg ttttcttaac atcgcggcat gcctacaagc    180



atcgtggagg tccgtgactt tcacgcatac aacaaacatt aaccaaggag gaaacagct     239





<210> 102

<211> 350

<212> DNA

<213> Artificial Sequence





<220> 

<223> PmglB_16UTR promoter sequence



<400> 102

tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc      60



aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc     120



tatgattgca ccgttttaac gttgtaaccc gtatgtaaca gtgaataatc acttttgccg     180



aggtaacagc gtcataacaa caattaaagc cgttttctgg agcgttaccg ggcatggaag     240



aacgaatttt aaaaagtgag cttcggcgtt cagtaacact tcattaactc tactgccccg     300



ccgagcattt atctcaagca ctaccctgca taagcaagga ggaaacagct                350





<210> 103

<211> 189

<212> DNA

<213> Artificial Sequence





<220> 

<223> PglpA_16UTR promoter sequence



<400> 103

gaaaacattc ataaattaaa tgtgaattgc cgcacacatt attaaataag atttacaaaa     60



tgttcaaaat gacgcatgaa atcacgtttc actttcgaat tatgagcgaa tatgcgcgat    120



gcctacaagc atcgtggagg tccgtgactt tcacgcatac aacaaacatt aaccaaggag    180



gaaacagct                                                            189





