                         SEQUENCE LISTING

<110>  Wisconsin Alumni Research Foundation
       Galipeau, Jacques
       Paul, Pradyut
 
<120>  SYNTHETIC PROTEIN FOR INDUCING IMMUNE TOLERANCE

<130>  960296.04290

<150>  63/189,359
<151>  2021-05-17

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  709
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - PIDO fusion protein comprising mouse PD-L1

<400>  1

Met Arg Ile Phe Ala Gly Ile Ile Phe Thr Ala Cys Cys His Leu Leu 
1               5                   10                  15      


Arg Ala Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          


Gly Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Arg Glu Leu 
        35                  40                  45              


Asp Leu Leu Ala Leu Val Val Tyr Trp Glu Lys Glu Asp Glu Gln Val 
    50                  55                  60                  


Ile Gln Phe Val Ala Gly Glu Glu Asp Leu Lys Pro Gln His Ser Asn 
65                  70                  75                  80  


Phe Arg Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu Lys Gly Asn 
                85                  90                  95      


Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr 
            100                 105                 110         


Cys Cys Ile Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu 
        115                 120                 125             


Lys Val Asn Ala Pro Tyr Arg Lys Ile Asn Gln Arg Ile Ser Val Asp 
    130                 135                 140                 


Pro Ala Thr Ser Glu His Glu Leu Ile Cys Gln Ala Glu Gly Tyr Pro 
145                 150                 155                 160 


Glu Ala Glu Val Ile Trp Thr Asn Ser Asp His Gln Pro Val Ser Gly 
                165                 170                 175     


Lys Arg Ser Val Thr Thr Ser Arg Thr Glu Gly Met Leu Leu Asn Val 
            180                 185                 190         


Thr Ser Ser Leu Arg Val Asn Ala Thr Ala Asn Asp Val Phe Tyr Cys 
        195                 200                 205             


Thr Phe Trp Arg Ser Gln Pro Gly Gln Asn His Thr Ala Glu Leu Ile 
    210                 215                 220                 


Ile Pro Glu Leu Pro Ala Thr His Pro Pro Gln Asn Arg Thr His Trp 
225                 230                 235                 240 


Val Leu Leu Gly Ser Ile Leu Leu Phe Leu Ile Val Val Ser Thr Val 
                245                 250                 255     


Leu Leu Phe Leu Arg Lys Gln Val Arg Met Leu Asp Val Glu Lys Cys 
            260                 265                 270         


Gly Val Glu Asp Thr Ser Ser Lys Asn Arg Asn Asp Thr Gln Phe Glu 
        275                 280                 285             


Glu Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    290                 295                 300                 


Gly Ser Met Ala His Ala Met Glu Asn Ser Trp Thr Ile Ser Lys Glu 
305                 310                 315                 320 


Tyr His Ile Asp Glu Glu Val Gly Phe Ala Leu Pro Asn Pro Gln Glu 
                325                 330                 335     


Asn Leu Pro Asp Phe Tyr Asn Asp Trp Met Phe Ile Ala Lys His Leu 
            340                 345                 350         


Pro Asp Leu Ile Glu Ser Gly Gln Leu Arg Glu Arg Val Glu Lys Leu 
        355                 360                 365             


Asn Met Leu Ser Ile Asp His Leu Thr Asp His Lys Ser Gln Arg Leu 
    370                 375                 380                 


Ala Arg Leu Val Leu Gly Cys Ile Thr Met Ala Tyr Val Trp Gly Lys 
385                 390                 395                 400 


Gly His Gly Asp Val Arg Lys Val Leu Pro Arg Asn Ile Ala Val Pro 
                405                 410                 415     


Tyr Cys Gln Leu Ser Lys Lys Leu Glu Leu Pro Pro Ile Leu Val Tyr 
            420                 425                 430         


Ala Asp Cys Val Leu Ala Asn Trp Lys Lys Lys Asp Pro Asn Lys Pro 
        435                 440                 445             


Leu Thr Tyr Glu Asn Met Asp Val Leu Phe Ser Phe Arg Asp Gly Asp 
    450                 455                 460                 


Cys Ser Lys Gly Phe Phe Leu Val Ser Leu Leu Val Glu Ile Ala Ala 
465                 470                 475                 480 


Ala Ser Ala Ile Lys Val Ile Pro Thr Val Phe Lys Ala Met Gln Met 
                485                 490                 495     


Gln Glu Arg Asp Thr Leu Leu Lys Ala Leu Leu Glu Ile Ala Ser Cys 
            500                 505                 510         


Leu Glu Lys Ala Leu Gln Val Phe His Gln Ile His Asp His Val Asn 
        515                 520                 525             


Pro Lys Ala Phe Phe Ser Val Leu Arg Ile Tyr Leu Ser Gly Trp Lys 
    530                 535                 540                 


Gly Asn Pro Gln Leu Ser Asp Gly Leu Val Tyr Glu Gly Phe Trp Glu 
545                 550                 555                 560 


Asp Pro Lys Glu Phe Ala Gly Gly Ser Ala Gly Gln Ser Ser Val Phe 
                565                 570                 575     


Gln Cys Phe Asp Val Leu Leu Gly Ile Gln Gln Thr Ala Gly Gly Gly 
            580                 585                 590         


His Ala Ala Gln Phe Leu Gln Asp Met Arg Arg Tyr Met Pro Pro Ala 
        595                 600                 605             


His Arg Asn Phe Leu Cys Ser Leu Glu Ser Asn Pro Ser Val Arg Glu 
    610                 615                 620                 


Phe Val Leu Ser Lys Gly Asp Ala Gly Leu Arg Glu Ala Tyr Asp Ala 
625                 630                 635                 640 


Cys Val Lys Ala Leu Val Ser Leu Arg Ser Tyr His Leu Gln Ile Val 
                645                 650                 655     


Thr Lys Tyr Ile Leu Ile Pro Ala Ser Gln Gln Pro Lys Glu Asn Lys 
            660                 665                 670         


Thr Ser Glu Asp Pro Ser Lys Leu Glu Ala Lys Gly Thr Gly Gly Thr 
        675                 680                 685             


Asp Leu Met Asn Phe Leu Lys Thr Val Arg Ser Thr Thr Glu Lys Ser 
    690                 695                 700                 


Leu Leu Lys Glu Gly 
705                 


<210>  2
<211>  290
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(290)
<223>  Full-length mouse PD-L1 protein

<220>
<221>  MISC_FEATURE
<222>  (121)..(125)
<223>  ADYKR sequence required for PD-1 binding domain

<400>  2

Met Arg Ile Phe Ala Gly Ile Ile Phe Thr Ala Cys Cys His Leu Leu 
1               5                   10                  15      


Arg Ala Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          


Gly Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Arg Glu Leu 
        35                  40                  45              


Asp Leu Leu Ala Leu Val Val Tyr Trp Glu Lys Glu Asp Glu Gln Val 
    50                  55                  60                  


Ile Gln Phe Val Ala Gly Glu Glu Asp Leu Lys Pro Gln His Ser Asn 
65                  70                  75                  80  


Phe Arg Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu Lys Gly Asn 
                85                  90                  95      


Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr 
            100                 105                 110         


Cys Cys Ile Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu 
        115                 120                 125             


Lys Val Asn Ala Pro Tyr Arg Lys Ile Asn Gln Arg Ile Ser Val Asp 
    130                 135                 140                 


Pro Ala Thr Ser Glu His Glu Leu Ile Cys Gln Ala Glu Gly Tyr Pro 
145                 150                 155                 160 


Glu Ala Glu Val Ile Trp Thr Asn Ser Asp His Gln Pro Val Ser Gly 
                165                 170                 175     


Lys Arg Ser Val Thr Thr Ser Arg Thr Glu Gly Met Leu Leu Asn Val 
            180                 185                 190         


Thr Ser Ser Leu Arg Val Asn Ala Thr Ala Asn Asp Val Phe Tyr Cys 
        195                 200                 205             


Thr Phe Trp Arg Ser Gln Pro Gly Gln Asn His Thr Ala Glu Leu Ile 
    210                 215                 220                 


Ile Pro Glu Leu Pro Ala Thr His Pro Pro Gln Asn Arg Thr His Trp 
225                 230                 235                 240 


Val Leu Leu Gly Ser Ile Leu Leu Phe Leu Ile Val Val Ser Thr Val 
                245                 250                 255     


Leu Leu Phe Leu Arg Lys Gln Val Arg Met Leu Asp Val Glu Lys Cys 
            260                 265                 270         


Gly Val Glu Asp Thr Ser Ser Lys Asn Arg Asn Asp Thr Gln Phe Glu 
        275                 280                 285             


Glu Thr 
    290 


<210>  3
<211>  221
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(221)
<223>  Extracellular domain of mouse PD-L1 (amino acids 19-239 of SEQ ID
       NO: 2)

<400>  3

Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val Val Glu Tyr Gly Ser 
1               5                   10                  15      


Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Arg Glu Leu Asp Leu 
            20                  25                  30          


Leu Ala Leu Val Val Tyr Trp Glu Lys Glu Asp Glu Gln Val Ile Gln 
        35                  40                  45              


Phe Val Ala Gly Glu Glu Asp Leu Lys Pro Gln His Ser Asn Phe Arg 
    50                  55                  60                  


Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu Lys Gly Asn Ala Ala 
65                  70                  75                  80  


Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr Cys Cys 
                85                  90                  95      


Ile Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu Lys Val 
            100                 105                 110         


Asn Ala Pro Tyr Arg Lys Ile Asn Gln Arg Ile Ser Val Asp Pro Ala 
        115                 120                 125             


Thr Ser Glu His Glu Leu Ile Cys Gln Ala Glu Gly Tyr Pro Glu Ala 
    130                 135                 140                 


Glu Val Ile Trp Thr Asn Ser Asp His Gln Pro Val Ser Gly Lys Arg 
145                 150                 155                 160 


Ser Val Thr Thr Ser Arg Thr Glu Gly Met Leu Leu Asn Val Thr Ser 
                165                 170                 175     


Ser Leu Arg Val Asn Ala Thr Ala Asn Asp Val Phe Tyr Cys Thr Phe 
            180                 185                 190         


Trp Arg Ser Gln Pro Gly Gln Asn His Thr Ala Glu Leu Ile Ile Pro 
        195                 200                 205             


Glu Leu Pro Ala Thr His Pro Pro Gln Asn Arg Thr His 
    210                 215                 220     


<210>  4
<211>  18
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(18)
<223>  Signal peptide of mouse PD-L1 (amino acids 1-18 of SEQ ID NO: 2; 
       cleaved off in mature protein)

<400>  4

Met Arg Ile Phe Ala Gly Ile Ile Phe Thr Ala Cys Cys His Leu Leu 
1               5                   10                  15      


Arg Ala 
        


<210>  5
<211>  21
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  Transmembrane domain of mouse PD-L1 (amino acids 240-260 of SEQ 
       ID NO: 2)

<400>  5

Trp Val Leu Leu Gly Ser Ile Leu Leu Phe Leu Ile Val Val Ser Thr 
1               5                   10                  15      


Val Leu Leu Phe Leu 
            20      


<210>  6
<211>  290
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(290)
<223>  Full-length human PD-L1 protein

<220>
<221>  MISC_FEATURE
<222>  (121)..(125)
<223>  ADYKR sequence required for PD-1 binding domain

<400>  6

Met Arg Ile Phe Ala Val Phe Ile Phe Met Thr Tyr Trp His Leu Leu 
1               5                   10                  15      


Asn Ala Phe Thr Val Thr Val Pro Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          


Gly Ser Asn Met Thr Ile Glu Cys Lys Phe Pro Val Glu Lys Gln Leu 
        35                  40                  45              


Asp Leu Ala Ala Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Asn Ile 
    50                  55                  60                  


Ile Gln Phe Val His Gly Glu Glu Asp Leu Lys Val Gln His Ser Ser 
65                  70                  75                  80  


Tyr Arg Gln Arg Ala Arg Leu Leu Lys Asp Gln Leu Ser Leu Gly Asn 
                85                  90                  95      


Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr 
            100                 105                 110         


Arg Cys Met Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Val 
        115                 120                 125             


Lys Val Asn Ala Pro Tyr Asn Lys Ile Asn Gln Arg Ile Leu Val Val 
    130                 135                 140                 


Asp Pro Val Thr Ser Glu His Glu Leu Thr Cys Gln Ala Glu Gly Tyr 
145                 150                 155                 160 


Pro Lys Ala Glu Val Ile Trp Thr Ser Ser Asp His Gln Val Leu Ser 
                165                 170                 175     


Gly Lys Thr Thr Thr Thr Asn Ser Lys Arg Glu Glu Lys Leu Phe Asn 
            180                 185                 190         


Val Thr Ser Thr Leu Arg Ile Asn Thr Thr Thr Asn Glu Ile Phe Tyr 
        195                 200                 205             


Cys Thr Phe Arg Arg Leu Asp Pro Glu Glu Asn His Thr Ala Glu Leu 
    210                 215                 220                 


Val Ile Pro Glu Leu Pro Leu Ala His Pro Pro Asn Glu Arg Thr His 
225                 230                 235                 240 


Leu Val Ile Leu Gly Ala Ile Leu Leu Cys Leu Gly Val Ala Leu Thr 
                245                 250                 255     


Phe Ile Phe Arg Leu Arg Lys Gly Arg Met Met Asp Val Lys Lys Cys 
            260                 265                 270         


Gly Ile Gln Asp Thr Asn Ser Lys Lys Gln Ser Asp Thr His Leu Glu 
        275                 280                 285             


Glu Thr 
    290 


<210>  7
<211>  220
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(220)
<223>  Extracellular domain of human PD-L1 (amino acids 19-238 of SEQ ID
       NO:6)

<400>  7

Phe Thr Val Thr Val Pro Lys Asp Leu Tyr Val Val Glu Tyr Gly Ser 
1               5                   10                  15      


Asn Met Thr Ile Glu Cys Lys Phe Pro Val Glu Lys Gln Leu Asp Leu 
            20                  25                  30          


Ala Ala Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Asn Ile Ile Gln 
        35                  40                  45              


Phe Val His Gly Glu Glu Asp Leu Lys Val Gln His Ser Ser Tyr Arg 
    50                  55                  60                  


Gln Arg Ala Arg Leu Leu Lys Asp Gln Leu Ser Leu Gly Asn Ala Ala 
65                  70                  75                  80  


Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr Arg Cys 
                85                  90                  95      


Met Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Val Lys Val 
            100                 105                 110         


Asn Ala Pro Tyr Asn Lys Ile Asn Gln Arg Ile Leu Val Val Asp Pro 
        115                 120                 125             


Val Thr Ser Glu His Glu Leu Thr Cys Gln Ala Glu Gly Tyr Pro Lys 
    130                 135                 140                 


Ala Glu Val Ile Trp Thr Ser Ser Asp His Gln Val Leu Ser Gly Lys 
145                 150                 155                 160 


Thr Thr Thr Thr Asn Ser Lys Arg Glu Glu Lys Leu Phe Asn Val Thr 
                165                 170                 175     


Ser Thr Leu Arg Ile Asn Thr Thr Thr Asn Glu Ile Phe Tyr Cys Thr 
            180                 185                 190         


Phe Arg Arg Leu Asp Pro Glu Glu Asn His Thr Ala Glu Leu Val Ile 
        195                 200                 205             


Pro Glu Leu Pro Leu Ala His Pro Pro Asn Glu Arg 
    210                 215                 220 


<210>  8
<211>  18
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(18)
<223>  Signal peptide of human PD-L1 (amino acids 1-18 of SEQ ID NO:6; 
       cleaved off in mature protein)

<400>  8

Met Arg Ile Phe Ala Val Phe Ile Phe Met Thr Tyr Trp His Leu Leu 
1               5                   10                  15      


Asn Ala 
        


<210>  9
<211>  21
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(21)
<223>  Transmembrane domain of human PD-L1 (amino acids 239-259 of SEQ 
       ID NO:6)

<400>  9

Thr His Leu Val Ile Leu Gly Ala Ile Leu Leu Cys Leu Gly Val Ala 
1               5                   10                  15      


Leu Thr Phe Ile Phe 
            20      


<210>  10
<211>  403
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(403)
<223>  Full-length human IDO protein

<220>
<221>  MISC_FEATURE
<222>  (226)..(231)
<223>  FFXXXR sequence required for IDO catalytic activity

<400>  10

Met Ala His Ala Met Glu Asn Ser Trp Thr Ile Ser Lys Glu Tyr His 
1               5                   10                  15      


Ile Asp Glu Glu Val Gly Phe Ala Leu Pro Asn Pro Gln Glu Asn Leu 
            20                  25                  30          


Pro Asp Phe Tyr Asn Asp Trp Met Phe Ile Ala Lys His Leu Pro Asp 
        35                  40                  45              


Leu Ile Glu Ser Gly Gln Leu Arg Glu Arg Val Glu Lys Leu Asn Met 
    50                  55                  60                  


Leu Ser Ile Asp His Leu Thr Asp His Lys Ser Gln Arg Leu Ala Arg 
65                  70                  75                  80  


Leu Val Leu Gly Cys Ile Thr Met Ala Tyr Val Trp Gly Lys Gly His 
                85                  90                  95      


Gly Asp Val Arg Lys Val Leu Pro Arg Asn Ile Ala Val Pro Tyr Cys 
            100                 105                 110         


Gln Leu Ser Lys Lys Leu Glu Leu Pro Pro Ile Leu Val Tyr Ala Asp 
        115                 120                 125             


Cys Val Leu Ala Asn Trp Lys Lys Lys Asp Pro Asn Lys Pro Leu Thr 
    130                 135                 140                 


Tyr Glu Asn Met Asp Val Leu Phe Ser Phe Arg Asp Gly Asp Cys Ser 
145                 150                 155                 160 


Lys Gly Phe Phe Leu Val Ser Leu Leu Val Glu Ile Ala Ala Ala Ser 
                165                 170                 175     


Ala Ile Lys Val Ile Pro Thr Val Phe Lys Ala Met Gln Met Gln Glu 
            180                 185                 190         


Arg Asp Thr Leu Leu Lys Ala Leu Leu Glu Ile Ala Ser Cys Leu Glu 
        195                 200                 205             


Lys Ala Leu Gln Val Phe His Gln Ile His Asp His Val Asn Pro Lys 
    210                 215                 220                 


Ala Phe Phe Ser Val Leu Arg Ile Tyr Leu Ser Gly Trp Lys Gly Asn 
225                 230                 235                 240 


Pro Gln Leu Ser Asp Gly Leu Val Tyr Glu Gly Phe Trp Glu Asp Pro 
                245                 250                 255     


Lys Glu Phe Ala Gly Gly Ser Ala Gly Gln Ser Ser Val Phe Gln Cys 
            260                 265                 270         


Phe Asp Val Leu Leu Gly Ile Gln Gln Thr Ala Gly Gly Gly His Ala 
        275                 280                 285             


Ala Gln Phe Leu Gln Asp Met Arg Arg Tyr Met Pro Pro Ala His Arg 
    290                 295                 300                 


Asn Phe Leu Cys Ser Leu Glu Ser Asn Pro Ser Val Arg Glu Phe Val 
305                 310                 315                 320 


Leu Ser Lys Gly Asp Ala Gly Leu Arg Glu Ala Tyr Asp Ala Cys Val 
                325                 330                 335     


Lys Ala Leu Val Ser Leu Arg Ser Tyr His Leu Gln Ile Val Thr Lys 
            340                 345                 350         


Tyr Ile Leu Ile Pro Ala Ser Gln Gln Pro Lys Glu Asn Lys Thr Ser 
        355                 360                 365             


Glu Asp Pro Ser Lys Leu Glu Ala Lys Gly Thr Gly Gly Thr Asp Leu 
    370                 375                 380                 


Met Asn Phe Leu Lys Thr Val Arg Ser Thr Thr Glu Lys Ser Leu Leu 
385                 390                 395                 400 


Lys Glu Gly 
            


<210>  11
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - 3X GGGS linker

<400>  11

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15      


<210>  12
<211>  2130
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - DNA encoding PIDO fusion protein comprising mouse 
       PD-L1

<400>  12
atgaggatat ttgctggcat tatattcaca gcctgctgtc acttgctacg ggcgtttact       60

atcacggctc caaaggactt gtacgtggtg gagtatggca gcaacgtcac gatggagtgc      120

agattccctg tagaacggga gctggacctg cttgcgttag tggtgtactg ggaaaaggaa      180

gatgagcaag tgattcagtt tgtggcagga gaggaggacc ttaagcctca gcacagcaac      240

ttcaggggga gagcctcgct gccaaaggac cagcttttga agggaaatgc tgcccttcag      300

atcacagacg tcaagctgca ggacgcaggc gtttactgct gcataatcag ctacggtggt      360

gcggactaca agcgaatcac gctgaaagtc aatgccccat accgcaaaat caaccagaga      420

atttccgtgg atccagccac ttctgagcat gaactaatat gtcaggccga gggttatcca      480

gaagctgagg taatctggac aaacagtgac caccaacccg tgagtgggaa gagaagtgtc      540

accacttccc ggacagaggg gatgcttctc aatgtgacca gcagtctgag ggtcaacgcc      600

acagcgaatg atgttttcta ctgtacgttt tggagatcac agccagggca aaaccacaca      660

gcggagctga tcatcccaga actgcctgca acacatcctc cacagaacag gactcactgg      720

gtgcttctgg gatccatcct gttgttcctc attgtagtgt ccacggtcct cctcttcttg      780

agaaaacaag tgagaatgct agatgtggag aaatgtggcg ttgaagatac aagctcaaaa      840

aaccgaaatg atacacaatt cgaggagacg tctggtggcg gaggctcggg cggaggtggg      900

tcgggtggcg gcggatcaat ggcacacgct atggaaaact cctggacaat cagtaaagag      960

taccatattg atgaagaagt gggctttgct ctgccaaatc cacaggaaaa tctacctgat     1020

ttttataatg actggatgtt cattgctaaa catctgcctg atctcataga gtctggccag     1080

cttcgagaaa gagttgagaa gttaaacatg ctcagcattg atcatctcac agaccacaag     1140

tcacagcgcc ttgcacgtct agttctggga tgcatcacca tggcatatgt gtggggcaaa     1200

ggtcatggag atgtccgtaa ggtcttgcca agaaatattg ctgttcctta ctgccaactc     1260

tccaagaaac tggaactgcc tcctattttg gtttatgcag actgtgtctt ggcaaactgg     1320

aagaaaaagg atcctaataa gcccctgact tatgagaaca tggacgtttt gttctcattt     1380

cgtgatggag actgcagtaa aggattcttc ctggtctctc tattggtgga aatagcagct     1440

gcttctgcaa tcaaagtaat tcctactgta ttcaaggcaa tgcaaatgca agaacgggac     1500

actttgctaa aggcgctgtt ggaaatagct tcttgcttgg agaaagccct tcaagtgttt     1560

caccaaatcc acgatcatgt gaacccaaaa gcatttttca gtgttcttcg catatatttg     1620

tctggctgga aaggcaaccc ccagctatca gacggtctgg tgtatgaagg gttctgggaa     1680

gacccaaagg agtttgcagg gggcagtgca ggccaaagca gcgtctttca gtgctttgac     1740

gtcctgctgg gcatccagca gactgctggt ggaggacatg ctgctcagtt cctccaggac     1800

atgagaagat atatgccacc agctcacagg aacttcctgt gctcattaga gtcaaatccc     1860

tcagtccgtg agtttgtcct ttcaaaaggt gatgctggcc tgcgggaagc ttatgacgcc     1920

tgtgtgaaag ctctggtctc cctgaggagc taccatctgc aaatcgtgac taagtacatc     1980

ctgattcctg caagccagca gccaaaggag aataagacct ctgaagaccc ttcaaaactg     2040

gaagccaaag gaactggagg cactgattta atgaatttcc tgaagactgt aagaagtaca     2100

actgagaaat cccttttgaa ggaaggttaa                                      2130


<210>  13
<211>  11105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Lentiviral vector encoding PIDO fusion protein 
       comprising mouse PD-L1

<400>  13
aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca       60

tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga      120

tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt      180

gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg      240

gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc      300

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      360

taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg      420

aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt      480

gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg      540

actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga      600

attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta      660

aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta      720

gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga      780

tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg      840

atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt      900

aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga      960

caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc     1020

acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc     1080

tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct     1140

gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag     1200

ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca     1260

ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg     1320

ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa     1380

atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa     1440

ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga     1500

acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa     1560

ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat     1620

agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt     1680

tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg     1740

tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta     1800

gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata     1860

atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt     1920

actagtgatt atcggatcaa ctttgtatag aaaagttgcg ttacataact tacggtaaat     1980

ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaatagt aacgccaata     2040

gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta     2100

catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc     2160

gcctggcatt gtgcccagta catgacctta tgggactttc ctacttggca gtacatctac     2220

gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc     2280

atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca     2340

gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg     2400

cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag     2460

tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg     2520

gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc     2580

ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct     2640

cctccgggct gtaattagct gagcaagagg taagggttta agggatggtt ggttggtggg     2700

gtattaatgt ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggcaag     2760

tttgtacaaa aaagcaggct gccaccatga ggatatttgc tggcattata ttcacagcct     2820

gctgtcactt gctacgggcg tttactatca cggctccaaa ggacttgtac gtggtggagt     2880

atggcagcaa cgtcacgatg gagtgcagat tccctgtaga acgggagctg gacctgcttg     2940

cgttagtggt gtactgggaa aaggaagatg agcaagtgat tcagtttgtg gcaggagagg     3000

aggaccttaa gcctcagcac agcaacttca gggggagagc ctcgctgcca aaggaccagc     3060

ttttgaaggg aaatgctgcc cttcagatca cagacgtcaa gctgcaggac gcaggcgttt     3120

actgctgcat aatcagctac ggtggtgcgg actacaagcg aatcacgctg aaagtcaatg     3180

ccccataccg caaaatcaac cagagaattt ccgtggatcc agccacttct gagcatgaac     3240

taatatgtca ggccgagggt tatccagaag ctgaggtaat ctggacaaac agtgaccacc     3300

aacccgtgag tgggaagaga agtgtcacca cttcccggac agaggggatg cttctcaatg     3360

tgaccagcag tctgagggtc aacgccacag cgaatgatgt tttctactgt acgttttgga     3420

gatcacagcc agggcaaaac cacacagcgg agctgatcat cccagaactg cctgcaacac     3480

atcctccaca gaacaggact cactgggtgc ttctgggatc catcctgttg ttcctcattg     3540

tagtgtccac ggtcctcctc ttcttgagaa aacaagtgag aatgctagat gtggagaaat     3600

gtggcgttga agatacaagc tcaaaaaacc gaaatgatac acaattcgag gagacgtctg     3660

gtggcggagg ctcgggcgga ggtgggtcgg gtggcggcgg atcaatggca cacgctatgg     3720

aaaactcctg gacaatcagt aaagagtacc atattgatga agaagtgggc tttgctctgc     3780

caaatccaca ggaaaatcta cctgattttt ataatgactg gatgttcatt gctaaacatc     3840

tgcctgatct catagagtct ggccagcttc gagaaagagt tgagaagtta aacatgctca     3900

gcattgatca tctcacagac cacaagtcac agcgccttgc acgtctagtt ctgggatgca     3960

tcaccatggc atatgtgtgg ggcaaaggtc atggagatgt ccgtaaggtc ttgccaagaa     4020

atattgctgt tccttactgc caactctcca agaaactgga actgcctcct attttggttt     4080

atgcagactg tgtcttggca aactggaaga aaaaggatcc taataagccc ctgacttatg     4140

agaacatgga cgttttgttc tcatttcgtg atggagactg cagtaaagga ttcttcctgg     4200

tctctctatt ggtggaaata gcagctgctt ctgcaatcaa agtaattcct actgtattca     4260

aggcaatgca aatgcaagaa cgggacactt tgctaaaggc gctgttggaa atagcttctt     4320

gcttggagaa agcccttcaa gtgtttcacc aaatccacga tcatgtgaac ccaaaagcat     4380

ttttcagtgt tcttcgcata tatttgtctg gctggaaagg caacccccag ctatcagacg     4440

gtctggtgta tgaagggttc tgggaagacc caaaggagtt tgcagggggc agtgcaggcc     4500

aaagcagcgt ctttcagtgc tttgacgtcc tgctgggcat ccagcagact gctggtggag     4560

gacatgctgc tcagttcctc caggacatga gaagatatat gccaccagct cacaggaact     4620

tcctgtgctc attagagtca aatccctcag tccgtgagtt tgtcctttca aaaggtgatg     4680

ctggcctgcg ggaagcttat gacgcctgtg tgaaagctct ggtctccctg aggagctacc     4740

atctgcaaat cgtgactaag tacatcctga ttcctgcaag ccagcagcca aaggagaata     4800

agacctctga agacccttca aaactggaag ccaaaggaac tggaggcact gatttaatga     4860

atttcctgaa gactgtaaga agtacaactg agaaatccct tttgaaggaa ggttaaaccc     4920

agctttcttg tacaaagtgg tgataatcga attccgataa tcaacctctg gattacaaaa     4980

tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg     5040

ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct     5100

tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg     5160

gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct     5220

gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg     5280

ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg     5340

tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc     5400

tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc     5460

gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc     5520

ggatctccct ttgggccgcc tccccgcatc gggaattccc gcggttcgaa cgcgttgaca     5580

ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata     5640

tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga     5700

cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt     5760

ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt     5820

gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca     5880

ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt     5940

catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt     6000

tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca     6060

ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg     6120

cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc     6180

cactgcgcca ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag     6240

ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag     6300

ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac     6360

atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc     6420

cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac     6480

gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc     6540

tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa     6600

acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc     6660

agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc     6720

acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac     6780

tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg     6840

gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa tggctccgga     6900

gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg ccccatgacc     6960

gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc     7020

ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac     7080

atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc     7140

aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc     7200

gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg     7260

ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg     7320

tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc     7380

gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc     7440

tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc     7500

gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaggtacc     7560

tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg     7620

gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg     7680

gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact     7740

gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg     7800

tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag     7860

tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga     7920

gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac     7980

aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat     8040

caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc     8100

ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat     8160

gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt     8220

ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg     8280

ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg     8340

cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt     8400

cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg     8460

cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg     8520

ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc     8580

taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa     8640

aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc     8700

ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac     8760

tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt     8820

ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc     8880

ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt     8940

ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata     9000

atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt     9060

tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc     9120

tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat     9180

ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct     9240

atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca     9300

ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg     9360

catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa     9420

cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg     9480

ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga     9540

cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg     9600

cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt     9660

tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg     9720

agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc     9780

ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca     9840

gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc     9900

atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat     9960

cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc    10020

agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg    10080

ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct    10140

accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct    10200

tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct    10260

cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg    10320

gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc    10380

gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga    10440

gctatgagaa agcgccacgc ttcccgaaga gagaaaggcg gacaggtatc cggtaagcgg    10500

cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta    10560

tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg    10620

ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg    10680

ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat    10740

taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc    10800

agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc    10860

gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa    10920

cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc    10980

ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga    11040

ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca    11100

agctt                                                                11105


<210>  14
<211>  709
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - PIDO fusion protein comprising human PD-L1

<400>  14

Met Arg Ile Phe Ala Val Phe Ile Phe Met Thr Tyr Trp His Leu Leu 
1               5                   10                  15      


Asn Ala Phe Thr Val Thr Val Pro Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          


Gly Ser Asn Met Thr Ile Glu Cys Lys Phe Pro Val Glu Lys Gln Leu 
        35                  40                  45              


Asp Leu Ala Ala Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Asn Ile 
    50                  55                  60                  


Ile Gln Phe Val His Gly Glu Glu Asp Leu Lys Val Gln His Ser Ser 
65                  70                  75                  80  


Tyr Arg Gln Arg Ala Arg Leu Leu Lys Asp Gln Leu Ser Leu Gly Asn 
                85                  90                  95      


Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr 
            100                 105                 110         


Arg Cys Met Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Val 
        115                 120                 125             


Lys Val Asn Ala Pro Tyr Asn Lys Ile Asn Gln Arg Ile Leu Val Val 
    130                 135                 140                 


Asp Pro Val Thr Ser Glu His Glu Leu Thr Cys Gln Ala Glu Gly Tyr 
145                 150                 155                 160 


Pro Lys Ala Glu Val Ile Trp Thr Ser Ser Asp His Gln Val Leu Ser 
                165                 170                 175     


Gly Lys Thr Thr Thr Thr Asn Ser Lys Arg Glu Glu Lys Leu Phe Asn 
            180                 185                 190         


Val Thr Ser Thr Leu Arg Ile Asn Thr Thr Thr Asn Glu Ile Phe Tyr 
        195                 200                 205             


Cys Thr Phe Arg Arg Leu Asp Pro Glu Glu Asn His Thr Ala Glu Leu 
    210                 215                 220                 


Val Ile Pro Glu Leu Pro Leu Ala His Pro Pro Asn Glu Arg Thr His 
225                 230                 235                 240 


Leu Val Ile Leu Gly Ala Ile Leu Leu Cys Leu Gly Val Ala Leu Thr 
                245                 250                 255     


Phe Ile Phe Arg Leu Arg Lys Gly Arg Met Met Asp Val Lys Lys Cys 
            260                 265                 270         


Gly Ile Gln Asp Thr Asn Ser Lys Lys Gln Ser Asp Thr His Leu Glu 
        275                 280                 285             


Glu Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    290                 295                 300                 


Gly Ser Met Ala His Ala Met Glu Asn Ser Trp Thr Ile Ser Lys Glu 
305                 310                 315                 320 


Tyr His Ile Asp Glu Glu Val Gly Phe Ala Leu Pro Asn Pro Gln Glu 
                325                 330                 335     


Asn Leu Pro Asp Phe Tyr Asn Asp Trp Met Phe Ile Ala Lys His Leu 
            340                 345                 350         


Pro Asp Leu Ile Glu Ser Gly Gln Leu Arg Glu Arg Val Glu Lys Leu 
        355                 360                 365             


Asn Met Leu Ser Ile Asp His Leu Thr Asp His Lys Ser Gln Arg Leu 
    370                 375                 380                 


Ala Arg Leu Val Leu Gly Cys Ile Thr Met Ala Tyr Val Trp Gly Lys 
385                 390                 395                 400 


Gly His Gly Asp Val Arg Lys Val Leu Pro Arg Asn Ile Ala Val Pro 
                405                 410                 415     


Tyr Cys Gln Leu Ser Lys Lys Leu Glu Leu Pro Pro Ile Leu Val Tyr 
            420                 425                 430         


Ala Asp Cys Val Leu Ala Asn Trp Lys Lys Lys Asp Pro Asn Lys Pro 
        435                 440                 445             


Leu Thr Tyr Glu Asn Met Asp Val Leu Phe Ser Phe Arg Asp Gly Asp 
    450                 455                 460                 


Cys Ser Lys Gly Phe Phe Leu Val Ser Leu Leu Val Glu Ile Ala Ala 
465                 470                 475                 480 


Ala Ser Ala Ile Lys Val Ile Pro Thr Val Phe Lys Ala Met Gln Met 
                485                 490                 495     


Gln Glu Arg Asp Thr Leu Leu Lys Ala Leu Leu Glu Ile Ala Ser Cys 
            500                 505                 510         


Leu Glu Lys Ala Leu Gln Val Phe His Gln Ile His Asp His Val Asn 
        515                 520                 525             


Pro Lys Ala Phe Phe Ser Val Leu Arg Ile Tyr Leu Ser Gly Trp Lys 
    530                 535                 540                 


Gly Asn Pro Gln Leu Ser Asp Gly Leu Val Tyr Glu Gly Phe Trp Glu 
545                 550                 555                 560 


Asp Pro Lys Glu Phe Ala Gly Gly Ser Ala Gly Gln Ser Ser Val Phe 
                565                 570                 575     


Gln Cys Phe Asp Val Leu Leu Gly Ile Gln Gln Thr Ala Gly Gly Gly 
            580                 585                 590         


His Ala Ala Gln Phe Leu Gln Asp Met Arg Arg Tyr Met Pro Pro Ala 
        595                 600                 605             


His Arg Asn Phe Leu Cys Ser Leu Glu Ser Asn Pro Ser Val Arg Glu 
    610                 615                 620                 


Phe Val Leu Ser Lys Gly Asp Ala Gly Leu Arg Glu Ala Tyr Asp Ala 
625                 630                 635                 640 


Cys Val Lys Ala Leu Val Ser Leu Arg Ser Tyr His Leu Gln Ile Val 
                645                 650                 655     


Thr Lys Tyr Ile Leu Ile Pro Ala Ser Gln Gln Pro Lys Glu Asn Lys 
            660                 665                 670         


Thr Ser Glu Asp Pro Ser Lys Leu Glu Ala Lys Gly Thr Gly Gly Thr 
        675                 680                 685             


Asp Leu Met Asn Phe Leu Lys Thr Val Arg Ser Thr Thr Glu Lys Ser 
    690                 695                 700                 


Leu Leu Lys Glu Gly 
705                 


<210>  15
<211>  2130
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - DNA encoding PIDO fusion protein comprising human 
       PD-L1

<400>  15
atgaggatat ttgctgtctt tatattcatg acctactggc atttgctgaa cgcatttact       60

gtcacggttc ccaaggacct atatgtggta gagtatggta gcaatatgac aattgaatgc      120

aaattcccag tagaaaaaca attagacctg gctgcactaa ttgtctattg ggaaatggag      180

gataagaaca ttattcaatt tgtgcatgga gaggaagacc tgaaggttca gcatagtagc      240

tacagacaga gggcccggct gttgaaggac cagctctccc tgggaaatgc tgcacttcag      300

atcacagatg tgaaattgca ggatgcaggg gtgtaccgct gcatgatcag ctatggtggt      360

gccgactaca agcgaattac tgtgaaagtc aatgccccat acaacaaaat caaccaaaga      420

attttggttg tggatccagt cacctctgaa catgaactga catgtcaggc tgagggctac      480

cccaaggccg aagtcatctg gacaagcagt gaccatcaag tcctgagtgg taagaccacc      540

accaccaatt ccaagagaga ggagaagctt ttcaatgtga ccagcacact gagaatcaac      600

acaacaacta atgagatttt ctactgcact tttaggagat tagatcctga ggaaaaccat      660

acagctgaat tggtcatccc agaactacct ctggcacatc ctccaaatga aaggactcac      720

ttggtaattc tgggagccat cttattatgc cttggtgtag cactgacatt catcttccgt      780

ttaagaaaag ggagaatgat ggatgtgaaa aaatgtggca tccaagatac aaactcaaag      840

aagcaaagtg atacacattt ggaggagacg tctggtggcg gaggctcggg cggaggtggg      900

tcgggtggcg gcggatcaat ggcacacgct atggaaaact cctggacaat cagtaaagag      960

taccatattg atgaagaagt gggctttgct ctgccaaatc cacaggaaaa tctacctgat     1020

ttttataatg actggatgtt cattgctaaa catctgcctg atctcataga gtctggccag     1080

cttcgagaaa gagttgagaa gttaaacatg ctcagcattg atcatctcac agaccacaag     1140

tcacagcgcc ttgcacgtct agttctggga tgcatcacca tggcatatgt gtggggcaaa     1200

ggtcatggag atgtccgtaa ggtcttgcca agaaatattg ctgttcctta ctgccaactc     1260

tccaagaaac tggaactgcc tcctattttg gtttatgcag actgtgtctt ggcaaactgg     1320

aagaaaaagg atcctaataa gcccctgact tatgagaaca tggacgtttt gttctcattt     1380

cgtgatggag actgcagtaa aggattcttc ctggtctctc tattggtgga aatagcagct     1440

gcttctgcaa tcaaagtaat tcctactgta ttcaaggcaa tgcaaatgca agaacgggac     1500

actttgctaa aggcgctgtt ggaaatagct tcttgcttgg agaaagccct tcaagtgttt     1560

caccaaatcc acgatcatgt gaacccaaaa gcatttttca gtgttcttcg catatatttg     1620

tctggctgga aaggcaaccc ccagctatca gacggtctgg tgtatgaagg gttctgggaa     1680

gacccaaagg agtttgcagg gggcagtgca ggccaaagca gcgtctttca gtgctttgac     1740

gtcctgctgg gcatccagca gactgctggt ggaggacatg ctgctcagtt cctccaggac     1800

atgagaagat atatgccacc agctcacagg aacttcctgt gctcattaga gtcaaatccc     1860

tcagtccgtg agtttgtcct ttcaaaaggt gatgctggcc tgcgggaagc ttatgacgcc     1920

tgtgtgaaag ctctggtctc cctgaggagc taccatctgc aaatcgtgac taagtacatc     1980

ctgattcctg caagccagca gccaaaggag aataagacct ctgaagaccc ttcaaaactg     2040

gaagccaaag gaactggagg cactgattta atgaatttcc tgaagactgt aagaagtaca     2100

actgagaaat cccttttgaa ggaaggttaa                                      2130


<210>  16
<211>  10356
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Lentiviral vector encoding PIDO fusion protein 
       comprising human PD-L1

<400>  16
aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca       60

tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga      120

tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt      180

gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg      240

gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc      300

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      360

taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg      420

aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt      480

gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg      540

actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga      600

attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta      660

aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta      720

gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga      780

tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg      840

atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt      900

aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga      960

caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc     1020

acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc     1080

tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct     1140

gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag     1200

ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca     1260

ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg     1320

ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa     1380

atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa     1440

ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga     1500

acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa     1560

ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat     1620

agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt     1680

tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg     1740

tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta     1800

gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata     1860

atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt     1920

actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg     1980

cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg     2040

gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc     2100

tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt     2160

ttcgcaacgg gtttgccgcc agaacacagg caagtttgta caaaaaagca ggctgccacc     2220

atgaggatat ttgctgtctt tatattcatg acctactggc atttgctgaa cgcatttact     2280

gtcacggttc ccaaggacct atatgtggta gagtatggta gcaatatgac aattgaatgc     2340

aaattcccag tagaaaaaca attagacctg gctgcactaa ttgtctattg ggaaatggag     2400

gataagaaca ttattcaatt tgtgcatgga gaggaagacc tgaaggttca gcatagtagc     2460

tacagacaga gggcccggct gttgaaggac cagctctccc tgggaaatgc tgcacttcag     2520

atcacagatg tgaaattgca ggatgcaggg gtgtaccgct gcatgatcag ctatggtggt     2580

gccgactaca agcgaattac tgtgaaagtc aatgccccat acaacaaaat caaccaaaga     2640

attttggttg tggatccagt cacctctgaa catgaactga catgtcaggc tgagggctac     2700

cccaaggccg aagtcatctg gacaagcagt gaccatcaag tcctgagtgg taagaccacc     2760

accaccaatt ccaagagaga ggagaagctt ttcaatgtga ccagcacact gagaatcaac     2820

acaacaacta atgagatttt ctactgcact tttaggagat tagatcctga ggaaaaccat     2880

acagctgaat tggtcatccc agaactacct ctggcacatc ctccaaatga aaggactcac     2940

ttggtaattc tgggagccat cttattatgc cttggtgtag cactgacatt catcttccgt     3000

ttaagaaaag ggagaatgat ggatgtgaaa aaatgtggca tccaagatac aaactcaaag     3060

aagcaaagtg atacacattt ggaggagacg tctggtggcg gaggctcggg cggaggtggg     3120

tcgggtggcg gcggatcaat ggcacacgct atggaaaact cctggacaat cagtaaagag     3180

taccatattg atgaagaagt gggctttgct ctgccaaatc cacaggaaaa tctacctgat     3240

ttttataatg actggatgtt cattgctaaa catctgcctg atctcataga gtctggccag     3300

cttcgagaaa gagttgagaa gttaaacatg ctcagcattg atcatctcac agaccacaag     3360

tcacagcgcc ttgcacgtct agttctggga tgcatcacca tggcatatgt gtggggcaaa     3420

ggtcatggag atgtccgtaa ggtcttgcca agaaatattg ctgttcctta ctgccaactc     3480

tccaagaaac tggaactgcc tcctattttg gtttatgcag actgtgtctt ggcaaactgg     3540

aagaaaaagg atcctaataa gcccctgact tatgagaaca tggacgtttt gttctcattt     3600

cgtgatggag actgcagtaa aggattcttc ctggtctctc tattggtgga aatagcagct     3660

gcttctgcaa tcaaagtaat tcctactgta ttcaaggcaa tgcaaatgca agaacgggac     3720

actttgctaa aggcgctgtt ggaaatagct tcttgcttgg agaaagccct tcaagtgttt     3780

caccaaatcc acgatcatgt gaacccaaaa gcatttttca gtgttcttcg catatatttg     3840

tctggctgga aaggcaaccc ccagctatca gacggtctgg tgtatgaagg gttctgggaa     3900

gacccaaagg agtttgcagg gggcagtgca ggccaaagca gcgtctttca gtgctttgac     3960

gtcctgctgg gcatccagca gactgctggt ggaggacatg ctgctcagtt cctccaggac     4020

atgagaagat atatgccacc agctcacagg aacttcctgt gctcattaga gtcaaatccc     4080

tcagtccgtg agtttgtcct ttcaaaaggt gatgctggcc tgcgggaagc ttatgacgcc     4140

tgtgtgaaag ctctggtctc cctgaggagc taccatctgc aaatcgtgac taagtacatc     4200

ctgattcctg caagccagca gccaaaggag aataagacct ctgaagaccc ttcaaaactg     4260

gaagccaaag gaactggagg cactgattta atgaatttcc tgaagactgt aagaagtaca     4320

actgagaaat cccttttgaa ggaaggttaa acccagcttt cttgtacaaa gtggtgataa     4380

tcgaattccg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt     4440

aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct     4500

attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt     4560

tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac     4620

gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct     4680

ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca     4740

ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt     4800

ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc     4860

ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct     4920

cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg     4980

catcgggaat tcccgcggtt cgaacgcgtt gacattgatt attgactagt tattaatagt     5040

aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta     5100

cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga     5160

cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt     5220

tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta     5280

ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg     5340

actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt     5400

tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc     5460

accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat     5520

gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct     5580

atataagcag agctctctgg ctaactagag aacccactgc gccaccatgg tgagcaaggg     5640

cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg     5700

ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct     5760

gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct     5820

gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt     5880

caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg     5940

caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga     6000

gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa     6060

ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa     6120

cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca     6180

gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca     6240

gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt     6300

gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggctccg gagagggcag     6360

gggaagtctt ctaacatgcg gggacgtgga ggaaaatccc ggccccatgg ccaagccttt     6420

gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat     6480

ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg     6540

tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc     6600

tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg     6660

catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa     6720

agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc     6780

ctctggttat gtgtgggagg gctaaggtac ctttaagacc aatgacttac aaggcagctg     6840

tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac     6900

gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct     6960

gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag     7020

tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac     7080

ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt     7140

atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt     7200

ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac     7260

tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc     7320

tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc     7380

gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga     7440

gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc     7500

tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa     7560

aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt     7620

aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa     7680

tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg     7740

accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc     7800

gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga     7860

tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt     7920

gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat     7980

agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat     8040

ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa     8100

tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa     8160

atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca     8220

tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc     8280

aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc     8340

acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt     8400

acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt     8460

ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg     8520

ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact     8580

caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg     8640

ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga     8700

aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg     8760

aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa     8820

tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac     8880

aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc     8940

cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca     9000

ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga     9060

gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta     9120

agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc     9180

atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc     9240

cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt     9300

cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac     9360

cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct     9420

tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact     9480

tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg     9540

ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata     9600

aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga     9660

cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag     9720

agagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg     9780

agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac     9840

ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca     9900

acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg     9960

cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc    10020

gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa    10080

tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt    10140

ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt    10200

aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg    10260

gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc    10320

ctcactaaag ggaacaaaag ctggagctgc aagctt                              10356


<210>  17
<211>  708
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - PIDO fusion protein comprising canine PD-L1

<400>  17

Met Arg Met Phe Ser Val Phe Thr Phe Met Ala Tyr Cys His Leu Leu 
1               5                   10                  15      


Lys Ala Phe Thr Ile Thr Val Ser Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          


Gly Gly Asn Val Thr Met Glu Cys Lys Phe Pro Val Glu Lys Gln Leu 
        35                  40                  45              


Asn Leu Phe Ala Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Lys Ile 
    50                  55                  60                  


Ile Gln Phe Val Asn Gly Lys Glu Asp Leu Lys Val Gln His Ser Ser 
65                  70                  75                  80  


Tyr Ser Gln Arg Ala Gln Leu Leu Lys Asp Gln Leu Phe Leu Gly Lys 
                85                  90                  95      


Ala Ala Leu Gln Ile Thr Asp Val Arg Leu Gln Asp Ala Gly Val Tyr 
            100                 105                 110         


Cys Cys Leu Ile Gly Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu 
        115                 120                 125             


Lys Val His Ala Pro Tyr Arg Asn Ile Ser Gln Arg Ile Ser Val Asp 
    130                 135                 140                 


Pro Val Thr Ser Glu His Glu Leu Met Cys Gln Ala Glu Gly Tyr Pro 
145                 150                 155                 160 


Glu Ala Glu Val Ile Trp Thr Ser Ser Asp His Arg Val Leu Ser Gly 
                165                 170                 175     


Lys Thr Thr Ile Thr Asn Ser Asn Arg Glu Glu Lys Leu Phe Asn Val 
            180                 185                 190         


Thr Ser Thr Leu Asn Ile Asn Ala Thr Ala Asn Glu Ile Phe Tyr Cys 
        195                 200                 205             


Thr Phe Gln Arg Ser Gly Pro Glu Glu Asn Asn Thr Ala Glu Leu Val 
    210                 215                 220                 


Ile Pro Glu Arg Leu Pro Val Pro Ala Ser Glu Arg Thr His Phe Met 
225                 230                 235                 240 


Ile Leu Gly Pro Phe Leu Leu Leu Leu Gly Val Val Leu Ala Val Thr 
                245                 250                 255     


Phe Cys Leu Lys Lys His Gly Arg Met Met Asp Val Glu Lys Cys Cys 
            260                 265                 270         


Thr Arg Asp Arg Asn Ser Lys Lys Arg Asn Asp Ile Gln Phe Glu Glu 
        275                 280                 285             


Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    290                 295                 300                 


Ser Met Ala His Ala Met Glu Asn Ser Trp Thr Ile Ser Lys Glu Tyr 
305                 310                 315                 320 


His Ile Asp Glu Glu Val Gly Phe Ala Leu Pro Asn Pro Gln Glu Asn 
                325                 330                 335     


Leu Pro Asp Phe Tyr Asn Asp Trp Met Phe Ile Ala Lys His Leu Pro 
            340                 345                 350         


Asp Leu Ile Glu Ser Gly Gln Leu Arg Glu Arg Val Glu Lys Leu Asn 
        355                 360                 365             


Met Leu Ser Ile Asp His Leu Thr Asp His Lys Ser Gln Arg Leu Ala 
    370                 375                 380                 


Arg Leu Val Leu Gly Cys Ile Thr Met Ala Tyr Val Trp Gly Lys Gly 
385                 390                 395                 400 


His Gly Asp Val Arg Lys Val Leu Pro Arg Asn Ile Ala Val Pro Tyr 
                405                 410                 415     


Cys Gln Leu Ser Lys Lys Leu Glu Leu Pro Pro Ile Leu Val Tyr Ala 
            420                 425                 430         


Asp Cys Val Leu Ala Asn Trp Lys Lys Lys Asp Pro Asn Lys Pro Leu 
        435                 440                 445             


Thr Tyr Glu Asn Met Asp Val Leu Phe Ser Phe Arg Asp Gly Asp Cys 
    450                 455                 460                 


Ser Lys Gly Phe Phe Leu Val Ser Leu Leu Val Glu Ile Ala Ala Ala 
465                 470                 475                 480 


Ser Ala Ile Lys Val Ile Pro Thr Val Phe Lys Ala Met Gln Met Gln 
                485                 490                 495     


Glu Arg Asp Thr Leu Leu Lys Ala Leu Leu Glu Ile Ala Ser Cys Leu 
            500                 505                 510         


Glu Lys Ala Leu Gln Val Phe His Gln Ile His Asp His Val Asn Pro 
        515                 520                 525             


Lys Ala Phe Phe Ser Val Leu Arg Ile Tyr Leu Ser Gly Trp Lys Gly 
    530                 535                 540                 


Asn Pro Gln Leu Ser Asp Gly Leu Val Tyr Glu Gly Phe Trp Glu Asp 
545                 550                 555                 560 


Pro Lys Glu Phe Ala Gly Gly Ser Ala Gly Gln Ser Ser Val Phe Gln 
                565                 570                 575     


Cys Phe Asp Val Leu Leu Gly Ile Gln Gln Thr Ala Gly Gly Gly His 
            580                 585                 590         


Ala Ala Gln Phe Leu Gln Asp Met Arg Arg Tyr Met Pro Pro Ala His 
        595                 600                 605             


Arg Asn Phe Leu Cys Ser Leu Glu Ser Asn Pro Ser Val Arg Glu Phe 
    610                 615                 620                 


Val Leu Ser Lys Gly Asp Ala Gly Leu Arg Glu Ala Tyr Asp Ala Cys 
625                 630                 635                 640 


Val Lys Ala Leu Val Ser Leu Arg Ser Tyr His Leu Gln Ile Val Thr 
                645                 650                 655     


Lys Tyr Ile Leu Ile Pro Ala Ser Gln Gln Pro Lys Glu Asn Lys Thr 
            660                 665                 670         


Ser Glu Asp Pro Ser Lys Leu Glu Ala Lys Gly Thr Gly Gly Thr Asp 
        675                 680                 685             


Leu Met Asn Phe Leu Lys Thr Val Arg Ser Thr Thr Glu Lys Ser Leu 
    690                 695                 700                 


Leu Lys Glu Gly 
705             


<210>  18
<211>  2127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - DNA encoding PIDO fusion protein comprising canine 
       PD-L1

<400>  18
atgagaatgt ttagtgtctt tacattcatg gcctactgcc atttgctaaa agcatttacg       60

atcacagttt ctaaggacct gtatgtggta gagtatggtg gcaatgtgac aatggaatgc      120

aaattcccgg tggaaaaaca gttaaacttg tttgcactaa tcgtctactg ggaaatggag      180

gataaaaaaa ttatacaatt tgtgaatgga aaggaagacc tgaaagttca gcacagcagc      240

tacagccaga gggctcagct attgaaggac cagctcttct tggggaaggc tgcgcttcag      300

atcacagatg tgagattgca ggatgcaggg gtttactgct gcttgatcgg ctatggcggt      360

gctgactaca agcggattac tttgaaagtt catgccccgt accgcaacat cagccaaaga      420

atttctgtgg atcctgtcac ctctgaacat gaactaatgt gtcaggctga gggttaccct      480

gaggctgaag tcatctggac aagcagtgac caccgagtcc tgagtggcaa aaccaccatc      540

actaattcca atagggaaga gaagcttttc aatgtgacca gcacgctgaa catcaatgca      600

acagctaatg agattttcta ctgcactttt caaagatcag gtcctgagga aaacaatact      660

gccgagttgg tcatcccaga acgactgccc gttccagcaa gtgagaggac tcatttcatg      720

attctgggac ctttcctgtt gcttcttggt gtagtcctgg cagtcacttt ctgtctaaaa      780

aaacatggga gaatgatgga tgtggaaaaa tgttgcaccc gagataggaa ctcaaagaaa      840

cgaaatgata tacaatttga agagacatct ggtggcggag gctcgggcgg aggtgggtcg      900

ggtggcggcg gatcaatggc acacgctatg gaaaactcct ggacaatcag taaagagtac      960

catattgatg aagaagtggg ctttgctctg ccaaatccac aggaaaatct acctgatttt     1020

tataatgact ggatgttcat tgctaaacat ctgcctgatc tcatagagtc tggccagctt     1080

cgagaaagag ttgagaagtt aaacatgctc agcattgatc atctcacaga ccacaagtca     1140

cagcgccttg cacgtctagt tctgggatgc atcaccatgg catatgtgtg gggcaaaggt     1200

catggagatg tccgtaaggt cttgccaaga aatattgctg ttccttactg ccaactctcc     1260

aagaaactgg aactgcctcc tattttggtt tatgcagact gtgtcttggc aaactggaag     1320

aaaaaggatc ctaataagcc cctgacttat gagaacatgg acgttttgtt ctcatttcgt     1380

gatggagact gcagtaaagg attcttcctg gtctctctat tggtggaaat agcagctgct     1440

tctgcaatca aagtaattcc tactgtattc aaggcaatgc aaatgcaaga acgggacact     1500

ttgctaaagg cgctgttgga aatagcttct tgcttggaga aagcccttca agtgtttcac     1560

caaatccacg atcatgtgaa cccaaaagca tttttcagtg ttcttcgcat atatttgtct     1620

ggctggaaag gcaaccccca gctatcagac ggtctggtgt atgaagggtt ctgggaagac     1680

ccaaaggagt ttgcaggggg cagtgcaggc caaagcagcg tctttcagtg ctttgacgtc     1740

ctgctgggca tccagcagac tgctggtgga ggacatgctg ctcagttcct ccaggacatg     1800

agaagatata tgccaccagc tcacaggaac ttcctgtgct cattagagtc aaatccctca     1860

gtccgtgagt ttgtcctttc aaaaggtgat gctggcctgc gggaagctta tgacgcctgt     1920

gtgaaagctc tggtctccct gaggagctac catctgcaaa tcgtgactaa gtacatcctg     1980

attcctgcaa gccagcagcc aaaggagaat aagacctctg aagacccttc aaaactggaa     2040

gccaaaggaa ctggaggcac tgatttaatg aatttcctga agactgtaag aagtacaact     2100

gagaaatccc ttttgaagga aggttaa                                         2127


<210>  19
<211>  10353
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Lentiviral vector encoding PIDO fusion protein 
       comprising canine PD-L1

<400>  19
aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca       60

tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga      120

tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt      180

gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg      240

gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc      300

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      360

taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg      420

aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt      480

gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg      540

actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga      600

attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta      660

aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta      720

gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga      780

tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg      840

atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt      900

aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga      960

caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc     1020

acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc     1080

tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct     1140

gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag     1200

ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca     1260

ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg     1320

ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa     1380

atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa     1440

ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga     1500

acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa     1560

ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat     1620

agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt     1680

tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg     1740

tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta     1800

gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata     1860

atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt     1920

actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg     1980

cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg     2040

gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc     2100

tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt     2160

ttcgcaacgg gtttgccgcc agaacacagg caagtttgta caaaaaagca ggctgccacc     2220

atgagaatgt ttagtgtctt tacattcatg gcctactgcc atttgctaaa agcatttacg     2280

atcacagttt ctaaggacct gtatgtggta gagtatggtg gcaatgtgac aatggaatgc     2340

aaattcccgg tggaaaaaca gttaaacttg tttgcactaa tcgtctactg ggaaatggag     2400

gataaaaaaa ttatacaatt tgtgaatgga aaggaagacc tgaaagttca gcacagcagc     2460

tacagccaga gggctcagct attgaaggac cagctcttct tggggaaggc tgcgcttcag     2520

atcacagatg tgagattgca ggatgcaggg gtttactgct gcttgatcgg ctatggcggt     2580

gctgactaca agcggattac tttgaaagtt catgccccgt accgcaacat cagccaaaga     2640

atttctgtgg atcctgtcac ctctgaacat gaactaatgt gtcaggctga gggttaccct     2700

gaggctgaag tcatctggac aagcagtgac caccgagtcc tgagtggcaa aaccaccatc     2760

actaattcca atagggaaga gaagcttttc aatgtgacca gcacgctgaa catcaatgca     2820

acagctaatg agattttcta ctgcactttt caaagatcag gtcctgagga aaacaatact     2880

gccgagttgg tcatcccaga acgactgccc gttccagcaa gtgagaggac tcatttcatg     2940

attctgggac ctttcctgtt gcttcttggt gtagtcctgg cagtcacttt ctgtctaaaa     3000

aaacatggga gaatgatgga tgtggaaaaa tgttgcaccc gagataggaa ctcaaagaaa     3060

cgaaatgata tacaatttga agagacatct ggtggcggag gctcgggcgg aggtgggtcg     3120

ggtggcggcg gatcaatggc acacgctatg gaaaactcct ggacaatcag taaagagtac     3180

catattgatg aagaagtggg ctttgctctg ccaaatccac aggaaaatct acctgatttt     3240

tataatgact ggatgttcat tgctaaacat ctgcctgatc tcatagagtc tggccagctt     3300

cgagaaagag ttgagaagtt aaacatgctc agcattgatc atctcacaga ccacaagtca     3360

cagcgccttg cacgtctagt tctgggatgc atcaccatgg catatgtgtg gggcaaaggt     3420

catggagatg tccgtaaggt cttgccaaga aatattgctg ttccttactg ccaactctcc     3480

aagaaactgg aactgcctcc tattttggtt tatgcagact gtgtcttggc aaactggaag     3540

aaaaaggatc ctaataagcc cctgacttat gagaacatgg acgttttgtt ctcatttcgt     3600

gatggagact gcagtaaagg attcttcctg gtctctctat tggtggaaat agcagctgct     3660

tctgcaatca aagtaattcc tactgtattc aaggcaatgc aaatgcaaga acgggacact     3720

ttgctaaagg cgctgttgga aatagcttct tgcttggaga aagcccttca agtgtttcac     3780

caaatccacg atcatgtgaa cccaaaagca tttttcagtg ttcttcgcat atatttgtct     3840

ggctggaaag gcaaccccca gctatcagac ggtctggtgt atgaagggtt ctgggaagac     3900

ccaaaggagt ttgcaggggg cagtgcaggc caaagcagcg tctttcagtg ctttgacgtc     3960

ctgctgggca tccagcagac tgctggtgga ggacatgctg ctcagttcct ccaggacatg     4020

agaagatata tgccaccagc tcacaggaac ttcctgtgct cattagagtc aaatccctca     4080

gtccgtgagt ttgtcctttc aaaaggtgat gctggcctgc gggaagctta tgacgcctgt     4140

gtgaaagctc tggtctccct gaggagctac catctgcaaa tcgtgactaa gtacatcctg     4200

attcctgcaa gccagcagcc aaaggagaat aagacctctg aagacccttc aaaactggaa     4260

gccaaaggaa ctggaggcac tgatttaatg aatttcctga agactgtaag aagtacaact     4320

gagaaatccc ttttgaagga aggttaaacc cagctttctt gtacaaagtg gtgataatcg     4380

aattccgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac     4440

tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt     4500

gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat     4560

gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca     4620

acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc     4680

cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg     4740

gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca     4800

tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct     4860

tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt     4920

ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat     4980

cgggaattcc cgcggttcga acgcgttgac attgattatt gactagttat taatagtaat     5040

caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg     5100

taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt     5160

atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac     5220

ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg     5280

acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact     5340

ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt     5400

ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc     5460

ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc     5520

gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata     5580

taagcagagc tctctggcta actagagaac ccactgcgcc accatggtga gcaagggcga     5640

ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca     5700

caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa     5760

gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac     5820

ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa     5880

gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa     5940

ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct     6000

gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta     6060

caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt     6120

caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa     6180

cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc     6240

cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac     6300

cgccgccggg atcactctcg gcatggacga gctgtacaag ggctccggag agggcagggg     6360

aagtcttcta acatgcgggg acgtggagga aaatcccggc cccatggcca agcctttgtc     6420

tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc     6480

tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt     6540

caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc     6600

tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat     6660

cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc     6720

catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc     6780

tggttatgtg tgggagggct aaggtacctt taagaccaat gacttacaag gcagctgtag     6840

atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa     6900

gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg     6960

agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc     7020

ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct     7080

tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt     7140

tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata     7200

atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc     7260

attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat     7320

cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc     7380

ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct     7440

attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat     7500

agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac     7560

cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat     7620

agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg     7680

gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc     7740

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc     7800

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt     7860

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg     7920

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt     7980

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta     8040

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt     8100

aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg     8160

tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga     8220

gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac     8280

atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc     8340

cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca     8400

tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc     8460

caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg     8520

ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac     8580

cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca     8640

taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg     8700

agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac     8760

cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg     8820

caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat     8880

taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg     8940

ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg     9000

cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc     9060

aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc     9120

attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt     9180

tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt     9240

aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt     9300

gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag     9360

cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca     9420

gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca     9480

agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg     9540

ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg     9600

cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct     9660

acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaagaga     9720

gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc     9780

ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg     9840

agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg     9900

cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt     9960

tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc    10020

gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac    10080

gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc    10140

ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg    10200

caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat    10260

aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc    10320

actaaaggga acaaaagctg gagctgcaag ctt                                 10353


<210>  20
<211>  714
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - PIDO fusion protein comprising feline PD-L1

<400>  20

Met Arg Ile Phe Ser Val Phe Ala Phe Met Ala Tyr Cys His Leu Leu 
1               5                   10                  15      


Lys Ala Phe Thr Ile Thr Val Ser Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          


Gly Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Glu Gln Leu 
        35                  40                  45              


Asp Leu Val Ser Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Lys Ile 
    50                  55                  60                  


Ile Gln Phe Val Gln Gly Lys Glu Asp Leu Lys Val Gln His Arg Ser 
65                  70                  75                  80  


Tyr Ser Gln Arg Ala Gln Leu Leu Lys Asp Gln Leu Phe Leu Gly Lys 
                85                  90                  95      


Ala Ala Leu Gln Ile Thr Asn Val Thr Leu Glu Asp Ala Gly Val Tyr 
            100                 105                 110         


Cys Cys Leu Ile Gly Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu 
        115                 120                 125             


Lys Val His Ala Pro Tyr Arg Lys Ile Asn Gln Arg Ile Ser Val Asp 
    130                 135                 140                 


Pro Val Thr Ser Glu His Glu Leu Met Cys Gln Ala Glu Gly Tyr Pro 
145                 150                 155                 160 


Thr Ala Glu Val Ile Trp Thr Asn Ser Ala His Gln Val Leu Asn Gly 
                165                 170                 175     


Lys Thr Ile Ile Ser Val Ser Asn Met Glu Thr Lys Leu Phe Asn Val 
            180                 185                 190         


Thr Ser Thr Leu Arg Ile Asn Thr Thr Ala Asn Glu Ile Phe Tyr Cys 
        195                 200                 205             


Thr Phe Leu Gln Arg Ser Ser Pro Glu Gly Asn Ser Thr Ala Glu Leu 
    210                 215                 220                 


Val Ile Pro Glu Pro Phe Leu Val Pro Ala Asn Glu Arg Thr His Phe 
225                 230                 235                 240 


Met Ile Leu Gly Ala Ile Leu Leu Phe Leu Val Val Val Pro Ala Val 
                245                 250                 255     


Thr Phe Cys Leu Lys Lys Arg Asp Val Arg Thr Met Asp Val Glu Lys 
            260                 265                 270         


Cys Asp Thr Ala Asp Met Asn Ser Lys Lys Gln Asn Asp Leu Gln Phe 
        275                 280                 285             


Glu Glu Thr Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    290                 295                 300                 


Gly Gly Ser Met Ser His Asn Arg Ile Leu Pro Thr Lys Asn Ser Trp 
305                 310                 315                 320 


Lys Asn Leu Lys Glu Tyr His Ile Asp Glu Lys Val Gly Phe Val Leu 
                325                 330                 335     


Pro Thr Pro Gln Glu Glu Leu Pro Tyr Pro Tyr Asp Gln Trp Ile Ser 
            340                 345                 350         


Ile Ala Lys Asn Leu Pro Glu Leu Ile Asp Lys Asn Glu Leu Arg Lys 
        355                 360                 365             


Glu Val Glu Lys Leu Lys Met Val Ser Ile Asp Asp Leu Lys Asp His 
    370                 375                 380                 


Lys Ser Gln Arg Leu Ala His Leu Val Leu Gly Tyr Ile Thr Met Ala 
385                 390                 395                 400 


Tyr Val Trp Asn Gln Gly Gly Glu Asp Val Cys Glu Val Leu Pro Lys 
                405                 410                 415     


Asn Ile Ala Val Pro Tyr Cys Glu Leu Ser Lys Lys Leu Asp Leu Pro 
            420                 425                 430         


Pro Ile Leu Val Tyr Ala Asp Cys Val Leu Ala Asn Trp Lys Lys Lys 
        435                 440                 445             


Asp Pro Asn Gly Pro Met Thr Tyr Glu Asn Met Asp Ile Leu Phe Ser 
    450                 455                 460                 


Phe Pro Gly Gly Asp Cys Gly Lys Gly Phe Phe Leu Val Ser Leu Leu 
465                 470                 475                 480 


Val Glu Ile Ala Ala Ala Ser Ala Ile Lys Val Ile Pro Asp Leu Leu 
                485                 490                 495     


Asn Ala Val Lys Cys Glu Asp His Asn Thr Leu Gln Arg Ala Leu Arg 
            500                 505                 510         


Tyr Ile Ala Ser Cys Leu Lys Gln Ala Lys Glu Glu Phe Lys Gln Ile 
        515                 520                 525             


His Glu Tyr Val Asp Pro Asn Thr Phe Phe Asn Val Leu Arg Ile Tyr 
    530                 535                 540                 


Leu Ser Gly Trp Lys Gly Asn Pro Leu Leu Pro Glu Gly Leu Lys Tyr 
545                 550                 555                 560 


Glu Gly Val Trp Glu Thr Pro Lys Lys Phe Ala Gly Gly Ser Ala Ala 
                565                 570                 575     


Gln Ser Ser Val Phe Gln Cys Phe Asp Val Leu Leu Gly Ile Gln Gln 
            580                 585                 590         


Ser Ser Gly Glu Glu Ser Ser Phe Lys Phe Leu Gln Glu Met Arg Glu 
        595                 600                 605             


Tyr Met Pro Leu Ala His Arg Lys Phe Leu Gln Ser Leu Glu Ser Ala 
    610                 615                 620                 


Pro Ser Val Arg Glu Phe Val Ile Ser Lys Gly Asp Ala Lys Leu Arg 
625                 630                 635                 640 


Ala Asp Tyr Asn Glu Cys Val Lys Ala Met Ala Asp Leu Arg Lys Tyr 
                645                 650                 655     


His Leu Lys Ile Val Ala Lys Tyr Ile Val Ile Pro Ser Lys Asn Lys 
            660                 665                 670         


His Lys Thr Asn Ser Thr Ser Glu Glu Thr Ser Glu Pro Glu Asn Lys 
        675                 680                 685             


Gly Thr Gly Gly Thr Ser Val Met Thr Phe Leu Lys Ser Val Arg Asn 
    690                 695                 700                 


Thr Thr Glu Lys Phe Leu Leu Asn Val Asp 
705                 710                 


<210>  21
<211>  2145
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - DNA encoding PIDO fusion protein comprising feline 
       PD-L1

<400>  21
atgaggatat ttagtgtctt tgcattcatg gcctactgtc atttgctgaa agcgtttacg       60

atcacagtgt ccaaggacct gtatgtggta gagtacggca gcaatgtgac aatggagtgc      120

agattccccg tagaagaaca attagacctg gtttcactga tcgtctactg ggaaatggag      180

gataagaaaa tcattcagtt tgtgcaaggg aaggaagacc tgaaagttca gcacagaagc      240

tacagtcaga gggcccagct gttgaaggac cagctcttcc tggggaaggc cgcgcttcag      300

atcacaaacg tgaccctgga ggatgccggg gtttactgct gcttgattgg ctatggcggt      360

gctgactata agcggattac tttgaaagtt catgccccat atcgaaaaat caaccaaaga      420

atttctgtgg atcctgtcac ctctgaacat gaactaatgt gtcaggctga gggttaccca      480

accgctgaag tcatctggac aaacagtgcc catcaagtcc tgaatggcaa aaccatcatc      540

tctgtttcca atatggagac aaagcttttc aatgtgacca gcacgctgag aatcaacaca      600

acggctaacg agattttcta ctgcactttt cttcaaagat caagtcccga gggaaacagt      660

actgctgagt tggtcatccc agaaccattt ctggttccag caaatgagag gactcacttc      720

atgattctag gagccatcct gttgtttctt gtcgtggtcc cggctgtcac tttctgtctg      780

aagaaacgag atgtacgaac gatggatgtg gaaaaatgtg acaccgcaga tatgaactca      840

aagaagcaaa atgatctaca atttgaggag acgtctggtg gcggaggctc gggcggaggt      900

gggtcgggtg gcggcggatc aatgtcacac aatagaatat tgcctacaaa aaattcttgg      960

aaaaatttga aagaatacca tatagatgaa aaggtgggct tcgttctgcc aactccacag     1020

gaggaactac cttaccctta tgatcaatgg atttccattg ctaaaaacct gcctgaactt     1080

attgacaaaa atgaactacg taaagaagtt gagaagttaa aaatggtcag tattgatgac     1140

ctcaaagacc acaagtcaca gcgccttgca catctggtcc tggggtatat caccatggcg     1200

tatgtgtgga accaaggcgg tgaagatgtt tgtgaggtct tgccaaagaa cattgctgtt     1260

ccttactgtg aactgtctaa gaagctggat ctgcctccta ttctggttta tgcagattgt     1320

gtcttggcaa actggaagaa aaaggatccc aatgggccca tgacttatga gaacatggac     1380

attctgtttt cgtttcctgg tggagactgc ggtaaaggat ttttcctggt ttctctgttg     1440

gtggaaatag cagcggcttc tgcaatcaaa gtaattcctg atttattgaa tgcagtaaaa     1500

tgtgaggacc acaatactct gcaaagggcg ctgcgttaca tagcttcttg tctgaaacaa     1560

gccaaggaag agtttaaaca aattcatgaa tatgtggacc caaacacatt tttcaatgtt     1620

cttcgcatat acttgtctgg ctggaaaggc aaccccctgc tgccagaggg tctgaagtat     1680

gaaggtgtct gggaaacccc aaagaagttt gcagggggta gtgcagccca aagcagtgtc     1740

ttccagtgct ttgatgttct gctgggcatc cagcagagtt ctggtgaaga atcttctttc     1800

aaattccttc aggaaatgag agaatatatg ccattagctc accggaaatt tctccagtca     1860

ttagagtcag ccccctcagt ccgtgagttt gttatttcaa aaggtgatgc taaactgaga     1920

gcagattata atgaatgtgt gaaagctatg gctgacctga gaaaatacca tttgaaaata     1980

gtagccaagt acatcgtgat tccttcaaag aacaagcaca agacaaactc aacatctgaa     2040

gaaacatcag aaccagaaaa taaaggaact ggaggcacta gtgtcatgac tttcctgaag     2100

agtgttagaa atacaactga gaaattcctg ctgaacgttg actaa                     2145


<210>  22
<211>  10937
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Lentiviral vector encoding PIDO fusion protein 
       comprising feline PD-L1

<400>  22
aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca       60

tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga      120

tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt      180

gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg      240

gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc      300

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      360

taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg      420

aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt      480

gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg      540

actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga      600

attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta      660

aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta      720

gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga      780

tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg      840

atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt      900

aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga      960

caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc     1020

acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc     1080

tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct     1140

gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag     1200

ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca     1260

ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg     1320

ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa     1380

atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa     1440

ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga     1500

acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa     1560

ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat     1620

agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt     1680

tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg     1740

tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta     1800

gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata     1860

atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt     1920

actagtgatt atcggatcaa ctttgtatag aaaagttgcg ttacataact tacggtaaat     1980

ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaatagt aacgccaata     2040

gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta     2100

catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc     2160

gcctggcatt gtgcccagta catgacctta tgggactttc ctacttggca gtacatctac     2220

gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc     2280

atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca     2340

gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg     2400

cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag     2460

tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg     2520

gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc     2580

ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct     2640

cctccgggct gtaattagct gagcaagagg taagggttta agggatggtt ggttggtggg     2700

gtattaatgt ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggcaag     2760

tttgtacaaa aaagcaggct gccaccatga ggatatttag tgtctttgca ttcatggcct     2820

actgtcattt gctgaaagcg tttacgatca cagtgtccaa ggacctgtat gtggtagagt     2880

acggcagcaa tgtgacaatg gagtgcagat tccccgtaga agaacaatta gacctggttt     2940

cactgatcgt ctactgggaa atggaggata agaaaatcat tcagtttgtg caagggaagg     3000

aagacctgaa agttcagcac agaagctaca gtcagagggc ccagctgttg aaggaccagc     3060

tcttcctggg gaaggccgcg cttcagatca caaacgtgac cctggaggat gccggggttt     3120

actgctgctt gattggctat ggcggtgctg actataagcg gattactttg aaagttcatg     3180

ccccatatcg aaaaatcaac caaagaattt ctgtggatcc tgtcacctct gaacatgaac     3240

taatgtgtca ggctgagggt tacccaaccg ctgaagtcat ctggacaaac agtgcccatc     3300

aagtcctgaa tggcaaaacc atcatctctg tttccaatat ggagacaaag cttttcaatg     3360

tgaccagcac gctgagaatc aacacaacgg ctaacgagat tttctactgc acttttcttc     3420

aaagatcaag tcccgaggga aacagtactg ctgagttggt catcccagaa ccatttctgg     3480

ttccagcaaa tgagaggact cacttcatga ttctaggagc catcctgttg tttcttgtcg     3540

tggtcccggc tgtcactttc tgtctgaaga aacgagatgt acgaacgatg gatgtggaaa     3600

aatgtgacac cgcagatatg aactcaaaga agcaaaatga tctacaattt gaggagacgt     3660

ctggtggcgg aggctcgggc ggaggtgggt cgggtggcgg cggatcaatg tcacacaata     3720

gaatattgcc tacaaaaaat tcttggaaaa atttgaaaga ataccatata gatgaaaagg     3780

tgggcttcgt tctgccaact ccacaggagg aactacctta cccttatgat caatggattt     3840

ccattgctaa aaacctgcct gaacttattg acaaaaatga actacgtaaa gaagttgaga     3900

agttaaaaat ggtcagtatt gatgacctca aagaccacaa gtcacagcgc cttgcacatc     3960

tggtcctggg gtatatcacc atggcgtatg tgtggaacca aggcggtgaa gatgtttgtg     4020

aggtcttgcc aaagaacatt gctgttcctt actgtgaact gtctaagaag ctggatctgc     4080

ctcctattct ggtttatgca gattgtgtct tggcaaactg gaagaaaaag gatcccaatg     4140

ggcccatgac ttatgagaac atggacattc tgttttcgtt tcctggtgga gactgcggta     4200

aaggattttt cctggtttct ctgttggtgg aaatagcagc ggcttctgca atcaaagtaa     4260

ttcctgattt attgaatgca gtaaaatgtg aggaccacaa tactctgcaa agggcgctgc     4320

gttacatagc ttcttgtctg aaacaagcca aggaagagtt taaacaaatt catgaatatg     4380

tggacccaaa cacatttttc aatgttcttc gcatatactt gtctggctgg aaaggcaacc     4440

ccctgctgcc agagggtctg aagtatgaag gtgtctggga aaccccaaag aagtttgcag     4500

ggggtagtgc agcccaaagc agtgtcttcc agtgctttga tgttctgctg ggcatccagc     4560

agagttctgg tgaagaatct tctttcaaat tccttcagga aatgagagaa tatatgccat     4620

tagctcaccg gaaatttctc cagtcattag agtcagcccc ctcagtccgt gagtttgtta     4680

tttcaaaagg tgatgctaaa ctgagagcag attataatga atgtgtgaaa gctatggctg     4740

acctgagaaa ataccatttg aaaatagtag ccaagtacat cgtgattcct tcaaagaaca     4800

agcacaagac aaactcaaca tctgaagaaa catcagaacc agaaaataaa ggaactggag     4860

gcactagtgt catgactttc ctgaagagtg ttagaaatac aactgagaaa ttcctgctga     4920

acgttgacta aacccagctt tcttgtacaa agtggtgata atcgaattcc gataatcaac     4980

ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta     5040

cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt     5100

tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg     5160

ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg     5220

gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca     5280

cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca     5340

ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg     5400

ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag     5460

cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc     5520

gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgggaa ttcccgcggt     5580

tcgaacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt     5640

agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg     5700

ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac     5760

gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt     5820

ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa     5880

atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta     5940

catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg     6000

gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg     6060

gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc     6120

attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg     6180

gctaactaga gaacccactg cgccaccatg gtgagcaagg gcgaggagct gttcaccggg     6240

gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc     6300

ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc     6360

ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc     6420

ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa     6480

ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc     6540

gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc     6600

aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc     6660

tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac     6720

atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac     6780

ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac     6840

cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact     6900

ctcggcatgg acgagctgta caagggctcc ggagagggca ggggaagtct tctaacatgc     6960

ggggacgtgg aggaaaatcc cggccccatg gccaagcctt tgtctcaaga agaatccacc     7020

ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc     7080

gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt     7140

actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc     7200

aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga     7260

cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt     7320

gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag     7380

ggctaaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt     7440

aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt     7500

tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact     7560

agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc     7620

ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa     7680

aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa     7740

atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag     7800

caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt     7860

gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg     7920

cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt     7980

ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga     8040

ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg     8100

cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac     8160

ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca     8220

ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg     8280

gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg     8340

ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc     8400

cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc     8460

tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga     8520

cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa     8580

ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga     8640

tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca     8700

aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat     8760

ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata     8820

aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct     8880

tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa     8940

agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa     9000

cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt     9060

taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg     9120

tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca     9180

tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa     9240

cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt     9300

gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc     9360

cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa     9420

actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga     9480

ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc     9540

tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga     9600

tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga     9660

acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga     9720

ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat     9780

ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt     9840

ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct     9900

gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc     9960

ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc    10020

aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc    10080

gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc    10140

gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg    10200

aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata    10260

cctacagcgt gagctatgag aaagcgccac gcttcccgaa gagagaaagg cggacaggta    10320

tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc    10380

ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg    10440

atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt    10500

cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt    10560

ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga    10620

gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc    10680

cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg    10740

cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca    10800

ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg    10860

aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa    10920

gctggagctg caagctt                                                   10937


<210>  23
<211>  289
<212>  PRT
<213>  Canis lupus


<220>
<221>  MISC_FEATURE
<222>  (1)..(289)
<223>  Full-length canine PD-L1 protein

<220>
<221>  MISC_FEATURE
<222>  (121)..(125)
<223>  ADYKR sequence required for PD-1 binding domain

<400>  23

Met Arg Met Phe Ser Val Phe Thr Phe Met Ala Tyr Cys His Leu Leu 
1               5                   10                  15      


Lys Ala Phe Thr Ile Thr Val Ser Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          


Gly Gly Asn Val Thr Met Glu Cys Lys Phe Pro Val Glu Lys Gln Leu 
        35                  40                  45              


Asn Leu Phe Ala Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Lys Ile 
    50                  55                  60                  


Ile Gln Phe Val Asn Gly Lys Glu Asp Leu Lys Val Gln His Ser Ser 
65                  70                  75                  80  


Tyr Ser Gln Arg Ala Gln Leu Leu Lys Asp Gln Leu Phe Leu Gly Lys 
                85                  90                  95      


Ala Ala Leu Gln Ile Thr Asp Val Arg Leu Gln Asp Ala Gly Val Tyr 
            100                 105                 110         


Cys Cys Leu Ile Gly Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu 
        115                 120                 125             


Lys Val His Ala Pro Tyr Arg Asn Ile Ser Gln Arg Ile Ser Val Asp 
    130                 135                 140                 


Pro Val Thr Ser Glu His Glu Leu Met Cys Gln Ala Glu Gly Tyr Pro 
145                 150                 155                 160 


Glu Ala Glu Val Ile Trp Thr Ser Ser Asp His Arg Val Leu Ser Gly 
                165                 170                 175     


Lys Thr Thr Ile Thr Asn Ser Asn Arg Glu Glu Lys Leu Phe Asn Val 
            180                 185                 190         


Thr Ser Thr Leu Asn Ile Asn Ala Thr Ala Asn Glu Ile Phe Tyr Cys 
        195                 200                 205             


Thr Phe Gln Arg Ser Gly Pro Glu Glu Asn Asn Thr Ala Glu Leu Val 
    210                 215                 220                 


Ile Pro Glu Arg Leu Pro Val Pro Ala Ser Glu Arg Thr His Phe Met 
225                 230                 235                 240 


Ile Leu Gly Pro Phe Leu Leu Leu Leu Gly Val Val Leu Ala Val Thr 
                245                 250                 255     


Phe Cys Leu Lys Lys His Gly Arg Met Met Asp Val Glu Lys Cys Cys 
            260                 265                 270         


Thr Arg Asp Arg Asn Ser Lys Lys Arg Asn Asp Ile Gln Phe Glu Glu 
        275                 280                 285             


Thr 
    


<210>  24
<211>  291
<212>  PRT
<213>  Felis catus


<220>
<221>  MISC_FEATURE
<222>  (1)..(291)
<223>  Full-length feline PD-L1 protein

<220>
<221>  MISC_FEATURE
<222>  (121)..(125)
<223>  ADYKR sequence required for PD-1 binding domain

<400>  24

Met Arg Ile Phe Ser Val Phe Ala Phe Met Ala Tyr Cys His Leu Leu 
1               5                   10                  15      


Lys Ala Phe Thr Ile Thr Val Ser Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          


Gly Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Glu Gln Leu 
        35                  40                  45              


Asp Leu Val Ser Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Lys Ile 
    50                  55                  60                  


Ile Gln Phe Val Gln Gly Lys Glu Asp Leu Lys Val Gln His Arg Ser 
65                  70                  75                  80  


Tyr Ser Gln Arg Ala Gln Leu Leu Lys Asp Gln Leu Phe Leu Gly Lys 
                85                  90                  95      


Ala Ala Leu Gln Ile Thr Asn Val Thr Leu Glu Asp Ala Gly Val Tyr 
            100                 105                 110         


Cys Cys Leu Ile Gly Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu 
        115                 120                 125             


Lys Val His Ala Pro Tyr Arg Lys Ile Asn Gln Arg Ile Ser Val Asp 
    130                 135                 140                 


Pro Val Thr Ser Glu His Glu Leu Met Cys Gln Ala Glu Gly Tyr Pro 
145                 150                 155                 160 


Thr Ala Glu Val Ile Trp Thr Asn Ser Ala His Gln Val Leu Asn Gly 
                165                 170                 175     


Lys Thr Ile Ile Ser Val Ser Asn Met Glu Thr Lys Leu Phe Asn Val 
            180                 185                 190         


Thr Ser Thr Leu Arg Ile Asn Thr Thr Ala Asn Glu Ile Phe Tyr Cys 
        195                 200                 205             


Thr Phe Leu Gln Arg Ser Ser Pro Glu Gly Asn Ser Thr Ala Glu Leu 
    210                 215                 220                 


Val Ile Pro Glu Pro Phe Leu Val Pro Ala Asn Glu Arg Thr His Phe 
225                 230                 235                 240 


Met Ile Leu Gly Ala Ile Leu Leu Phe Leu Val Val Val Pro Ala Val 
                245                 250                 255     


Thr Phe Cys Leu Lys Lys Arg Asp Val Arg Thr Met Asp Val Glu Lys 
            260                 265                 270         


Cys Asp Thr Ala Asp Met Asn Ser Lys Lys Gln Asn Asp Leu Gln Phe 
        275                 280                 285             


Glu Glu Thr 
    290     


