                         SEQUENCE LISTING

<110>  The University of Adelaide
       Shanghai Jiao Tong University
 
<120>  METHODS FOR IMPROVING PLANT YIELD

<130>  1229559

<150>  AU2021901643
<151>  2021-06-02

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  753
<212>  DNA
<213>  Hordeum vulgare

<400>  1
atgggtcgtg ggaaggtgga gatgaggcgg atcgagaaca agataagccg gcaggtgacc       60

ttcgccaagc gccggaacgg gctgctcaag aaggcctacg agctctcgct gctctgcgac      120

gccgaggtcg ccctcatcat cttctccggc cgcggccgcc tcttcgagtt ctcaagctcc      180

tcatgcatgt acaaaactct tgagagatac cgcacctgca actccaactc acaggaagca      240

acacctcagg tagaaaatga gattaattac caggaatatt taaagctgaa gacaagagtt      300

gaatttcttc aaagttcaca aagaaatatt ctcggtgagg atctgggccc acttagcatg      360

aaggagcttg accagataga gaaccaaata gatgcatccc tccagcatat caggtcaaaa      420

aagaatcaag tattactcga tcagctattt gaactgaaaa gtaaggagca agaattgcag      480

gatgaaaaca aagacttgag gaagaagctg caagacacca ccaccaccag ctgcggagag      540

aatgcggtcc atatgtcctg gcaagacggt gggcagagta gctccagaag gcatgccact      600

gagccttacc cgggagtact ccaacacccg gagcatgata cctccatgca aattgggtat      660

cctcaggcct acatggacca gctgaacaac agagatcaca tggcttcaca gcgccctggt      720

ggacatcctg gatcgtctgc aggttggata tga                                   753


<210>  2
<211>  250
<212>  PRT
<213>  Hordeum vulgare

<400>  2

Met Gly Arg Gly Lys Val Glu Met Arg Arg Ile Glu Asn Lys Ile Ser 
1               5                   10                  15      


Arg Gln Val Thr Phe Ala Lys Arg Arg Asn Gly Leu Leu Lys Lys Ala 
            20                  25                  30          


Tyr Glu Leu Ser Leu Leu Cys Asp Ala Glu Val Ala Leu Ile Ile Phe 
        35                  40                  45              


Ser Gly Arg Gly Arg Leu Phe Glu Phe Ser Ser Ser Ser Cys Met Tyr 
    50                  55                  60                  


Lys Thr Leu Glu Arg Tyr Arg Thr Cys Asn Ser Asn Ser Gln Glu Ala 
65                  70                  75                  80  


Thr Pro Gln Val Glu Asn Glu Ile Asn Tyr Gln Glu Tyr Leu Lys Leu 
                85                  90                  95      


Lys Thr Arg Val Glu Phe Leu Gln Ser Ser Gln Arg Asn Ile Leu Gly 
            100                 105                 110         


Glu Asp Leu Gly Pro Leu Ser Met Lys Glu Leu Asp Gln Ile Glu Asn 
        115                 120                 125             


Gln Ile Asp Ala Ser Leu Gln His Ile Arg Ser Lys Lys Asn Gln Val 
    130                 135                 140                 


Leu Leu Asp Gln Leu Phe Glu Leu Lys Ser Lys Glu Gln Glu Leu Gln 
145                 150                 155                 160 


Asp Glu Asn Lys Asp Leu Arg Lys Lys Leu Gln Asp Thr Thr Thr Thr 
                165                 170                 175     


Ser Cys Gly Glu Asn Ala Val His Met Ser Trp Gln Asp Gly Gly Gln 
            180                 185                 190         


Ser Ser Ser Arg Arg His Ala Thr Glu Pro Tyr Pro Gly Val Leu Gln 
        195                 200                 205             


His Pro Glu His Asp Thr Ser Met Gln Ile Gly Tyr Pro Gln Ala Tyr 
    210                 215                 220                 


Met Asp Gln Leu Asn Asn Arg Asp His Met Ala Ser Gln Arg Pro Gly 
225                 230                 235                 240 


Gly His Pro Gly Ser Ser Ala Gly Trp Ile 
                245                 250 


<210>  3
<211>  754
<212>  DNA
<213>  Hordeum vulgare

<400>  3
atgggtcgtg ggaaggtgga gatcggaggc ggatcgagaa caagataagc cggcaggtga       60

ccttcgccaa gcgccggaac gggctgctca agaaggccta cgagctctcg ctgctctgcg      120

acgccgaggt cgccctcatc atatctccgg ccgcggccgc ctcttcgagt tctcaagctc      180

ctcatgcatg tacaaaactc ttgagagata ccgcacctgc aactccaact cacaggaagc      240

aacacctcag gtagaaaatg agattaatta ccaggaatat ttaaagctga agacaagagt      300

tgaatttctt caaagttcac aaagaaatat tctcggtgag gatctgggcc cacttagcat      360

gaaggagctt gaccagatag agaaccaaat agatgcatcc ctccagcata tcaggtcaaa      420

aaagaatcaa gtattactcg atcagctatt tgaactgaaa agtaaggagc aagaattgca      480

ggatgaaaac aaagacttga ggaagaagct gcaagacacc accaccacca gctgcggaga      540

gaatgcggtc catatgtcct ggcaagacgg tgggcagagt agctccagaa ggcatgccac      600

tgagccttac ccgggagtac tccaacaccc ggagcatgat acctccatgc aaattgggta      660

tcctcaggcc tacatggacc agctgaacaa cagagatcac atggcttcac agcgccctgg      720

tggacatcct ggatcgtctg caggttggat atga                                  754


<210>  4
<211>  15
<212>  PRT
<213>  Hordeum vulgare

<400>  4

Met Gly Arg Gly Lys Val Glu Ile Gly Gly Gly Ser Arg Thr Arg 
1               5                   10                  15  


<210>  5
<211>  757
<212>  DNA
<213>  Hordeum vulgare

<400>  5
atgggtcgtg ggaaggtgga gccataggcg gatcgagaac aagataagcc ggcaggtgac       60

cttcgccaag cgccggaacg ggctgctcaa gaaggcctac gagctctcgc tgctctgcga      120

cgccgaggtc gccctcatca tcgatttctc cggccgcggc cgcctcttcg agttctcaag      180

ctcctcatgc atgtacaaaa ctcttgagag ataccgcacc tgcaactcca actcacagga      240

agcaacacct caggtagaaa atgagattaa ttaccaggaa tatttaaagc tgaagacaag      300

agttgaattt cttcaaagtt cacaaagaaa tattctcggt gaggatctgg gcccacttag      360

catgaaggag cttgaccaga tagagaacca aatagatgca tccctccagc atatcaggtc      420

aaaaaagaat caagtattac tcgatcagct atttgaactg aaaagtaagg agcaagaatt      480

gcaggatgaa aacaaagact tgaggaagaa gctgcaagac accaccacca ccagctgcgg      540

agagaatgcg gtccatatgt cctggcaaga cggtgggcag agtagctcca gaaggcatgc      600

cactgagcct tacccgggag tactccaaca cccggagcat gatacctcca tgcaaattgg      660

gtatcctcag gcctacatgg accagctgaa caacagagat cacatggctt cacagcgccc      720

tggtggacat cctggatcgt ctgcaggttg gatatga                               757


<210>  6
<211>  8
<212>  PRT
<213>  Hordeum vulgare

<400>  6

Met Gly Arg Gly Lys Val Glu Pro 
1               5               


<210>  7
<211>  748
<212>  DNA
<213>  Hordeum vulgare

<400>  7
atgggtcgtg ggaaggtgga gatcgaggcg gatcgagaac aagataagcc ggcaggtgac       60

cttcgccaag cgccggaacg ggctgctcaa gaaggcctac gagctctcgc tgctctgcga      120

cgccgaggtc gccctcatct ccggccgcgg ccgcctcttc gagttctcaa gctcctcatg      180

catgtacaaa actcttgaga gataccgcac ctgcaactcc aactcacagg aagcaacacc      240

tcaggtagaa aatgagatta attaccagga atatttaaag ctgaagacaa gagttgaatt      300

tcttcaaagt tcacaaagaa atattctcgg tgaggatctg ggcccactta gcatgaagga      360

gcttgaccag atagagaacc aaatagatgc atccctccag catatcaggt caaaaaagaa      420

tcaagtatta ctcgatcagc tatttgaact gaaaagtaag gagcaagaat tgcaggatga      480

aaacaaagac ttgaggaaga agctgcaaga caccaccacc accagctgcg gagagaatgc      540

ggtccatatg tcctggcaag acggtgggca gagtagctcc agaaggcatg ccactgagcc      600

ttacccggga gtactccaac acccggagca tgatacctcc atgcaaattg ggtatcctca      660

ggcctacatg gaccagctga acaacagaga tcacatggct tcacagcgcc ctggtggaca      720

tcctggatcg tctgcaggtt ggatatga                                         748


<210>  8
<211>  65
<212>  PRT
<213>  Hordeum vulgare

<400>  8

Met Gly Arg Gly Lys Val Glu Ile Glu Ala Asp Arg Glu Gln Asp Lys 
1               5                   10                  15      


Pro Ala Gly Asp Leu Arg Gln Ala Pro Glu Arg Ala Ala Gln Glu Gly 
            20                  25                  30          


Leu Arg Ala Leu Ala Ala Leu Arg Arg Arg Gly Arg Pro His Leu Arg 
        35                  40                  45              


Pro Arg Pro Pro Leu Arg Val Leu Lys Leu Leu Met His Val Gln Asn 
    50                  55                  60                  


Ser 
65  


<210>  9
<211>  757
<212>  DNA
<213>  Hordeum vulgare

<400>  9
atgggtcgtg ggaaggtgga gataggcgga tcgagaacaa gataagccgg caggtgacct       60

tcgccaagcg ccggaacggg ctgctcaaga aggcctacga gctctcgctg ctctgcgacg      120

ccgaggtcgc cctcatcatt aacctttctc cggccgcggc cgcctcttcg agttctcaag      180

ctcctcatgc atgtacaaaa ctcttgagag ataccgcacc tgcaactcca actcacagga      240

agcaacacct caggtagaaa atgagattaa ttaccaggaa tatttaaagc tgaagacaag      300

agttgaattt cttcaaagtt cacaaagaaa tattctcggt gaggatctgg gcccacttag      360

catgaaggag cttgaccaga tagagaacca aatagatgca tccctccagc atatcaggtc      420

aaaaaagaat caagtattac tcgatcagct atttgaactg aaaagtaagg agcaagaatt      480

gcaggatgaa aacaaagact tgaggaagaa gctgcaagac accaccacca ccagctgcgg      540

agagaatgcg gtccatatgt cctggcaaga cggtgggcag agtagctcca gaaggcatgc      600

cactgagcct tacccgggag tactccaaca cccggagcat gatacctcca tgcaaattgg      660

gtatcctcag gcctacatgg accagctgaa caacagagat cacatggctt cacagcgccc      720

tggtggacat cctggatcgt ctgcaggttg gatatga                               757


<210>  10
<211>  14
<212>  PRT
<213>  Hordeum vulgare

<400>  10

Met Gly Arg Gly Lys Val Glu Ile Gly Gly Ser Arg Thr Arg 
1               5                   10                  


<210>  11
<211>  757
<212>  DNA
<213>  Hordeum vulgare

<400>  11
atgggtcgtg ggaaggtgga gataagaggc ggatcgagaa caagataagc cggcaggtga       60

ccttcgccaa gcgccggaac gggctgctca agaaggccta cgagctctcg ctgctctgcg      120

acgccgaggt cgccctcatc atctattctc cggccgcggc cgcctcttcg agttctcaag      180

ctcctcatgc atgtacaaaa ctcttgagag ataccgcacc tgcaactcca actcacagga      240

agcaacacct caggtagaaa atgagattaa ttaccaggaa tatttaaagc tgaagacaag      300

agttgaattt cttcaaagtt cacaaagaaa tattctcggt gaggatctgg gcccacttag      360

catgaaggag cttgaccaga tagagaacca aatagatgca tccctccagc atatcaggtc      420

aaaaaagaat caagtattac tcgatcagct atttgaactg aaaagtaagg agcaagaatt      480

gcaggatgaa aacaaagact tgaggaagaa gctgcaagac accaccacca ccagctgcgg      540

agagaatgcg gtccatatgt cctggcaaga cggtgggcag agtagctcca gaaggcatgc      600

cactgagcct tacccgggag tactccaaca cccggagcat gatacctcca tgcaaattgg      660

gtatcctcag gcctacatgg accagctgaa caacagagat cacatggctt cacagcgccc      720

tggtggacat cctggatcgt ctgcaggttg gatatga                               757


<210>  12
<211>  15
<212>  PRT
<213>  Hordeum vulgare

<400>  12

Met Gly Arg Gly Lys Val Glu Ile Arg Gly Gly Ser Arg Thr Arg 
1               5                   10                  15  


<210>  13
<211>  754
<212>  DNA
<213>  Hordeum vulgare

<400>  13
atgggtcgtg ggaaggtgga gatgtgaggc ggatcgagaa caagataagc cggcaggtga       60

ccttcgccaa gcgccggaac gggctgctca agaaggccta cgagctctcg ctgctctgcg      120

acgccgaggt cgccctcatc atctctccgg ccgcggccgc ctcttcgagt tctcaagctc      180

ctcatgcatg tacaaaactc ttgagagata ccgcacctgc aactccaact cacaggaagc      240

aacacctcag gtagaaaatg agattaatta ccaggaatat ttaaagctga agacaagagt      300

tgaatttctt caaagttcac aaagaaatat tctcggtgag gatctgggcc cacttagcat      360

gaaggagctt gaccagatag agaaccaaat agatgcatcc ctccagcata tcaggtcaaa      420

aaagaatcaa gtattactcg atcagctatt tgaactgaaa agtaaggagc aagaattgca      480

ggatgaaaac aaagacttga ggaagaagct gcaagacacc accaccacca gctgcggaga      540

gaatgcggtc catatgtcct ggcaagacgg tgggcagagt agctccagaa ggcatgccac      600

tgagccttac ccgggagtac tccaacaccc ggagcatgat acctccatgc aaattgggta      660

tcctcaggcc tacatggacc agctgaacaa cagagatcac atggcttcac agcgccctgg      720

tggacatcct ggatcgtctg caggttggat atga                                  754


<210>  14
<211>  8
<212>  PRT
<213>  Hordeum vulgare

<400>  14

Met Gly Arg Gly Lys Val Glu Met 
1               5               


<210>  15
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA T1 sequence

<400>  15
gtgggaaggt ggagatgagg cgg                                               23


<210>  16
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA T2 sequence

<400>  16
gtcgccctca tcatcttctc cgg                                               23


<210>  17
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M1T1U6-sgF - expression cassette construction

<400>  17
tgggaaggtg gagatgaggg ttttagagct agaaat                                 36


<210>  18
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M1T1U6a-R - expression cassette construction

<400>  18
cctcatctcc accttcccac ggcagccaag ccagca                                 36


<210>  19
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M1T2U6-sgF - expression cassette construction

<400>  19
tcgccctcat catcttctcg ttttagagct agaaat                                 36


<210>  20
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M1T2U6b-R - expression cassette construction

<400>  20
gagaagatga tgagggcgac aacacaagcg gcagc                                  35


<210>  21
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U-F - expression cassette construction

<400>  21
ctccgtttta cctgtggaat cg                                                22


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gR-R - expression cassette construction

<400>  22
cggaggaaaa ttccatccac                                                   20


<210>  23
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pps-GGL - expression cassette construction

<400>  23
ttcagaggtc tctctcgact agtatggaat cggcagcaaa g                           41


<210>  24
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pgs-GG2 - expression cassette construction

<400>  24
agcgtgggtc tcgtcagggt ccatccactc caagctc                                37


<210>  25
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pps-GG2 - expression cassette construction

<400>  25
ttcagaggtc tctctgacac tggaatcggc agcaaagg                               38


<210>  26
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pgs-GGR - expression cassette construction

<400>  26
agcgtgggtc tcgaccgacg cgtatccatc cactccaagc t                           41


<210>  27
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PBL - for sequencing recombinant vector pYLCRISPR/Cas9-HvSEP1

<400>  27
gcgcgcggtc tcgctcgact agtatgg                                           27


<210>  28
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PBR - for sequencing recombinant vector pYLCRISPR/Cas9-HvSEP1

<400>  28
gcgcgcggtc tctaccgacg cgtatcc                                           27


<210>  29
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEP1seqF - for amplification of HvSEP1 T1 and T2 targets

<400>  29
gatcagacat cgctctgctg gc                                                22


<210>  30
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEP1seqR - for amplification of HvSEP1 T1 and T2 targets

<400>  30
gaggtttgtt tcgctagatc tcggtg                                            26


