                         SEQUENCE LISTING

<110>  Biocytogen PharmaceuticalsBeijingCo., Ltd.
 
<120>  GENETICALLY MODIFIED NON-HUMAN ANIMAL WITH HUMAN OR CHIMERIC BCMA

<130>  1

<150>  CN202110608267.9
<151>  2021-06-01

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  185
<212>  PRT
<213>  Mus musculus

<400>  1

Met Ala Gln Gln Cys Phe His Ser Glu Tyr Phe Asp Ser Leu Leu His 
1               5                   10                  15      


Ala Cys Lys Pro Cys His Leu Arg Cys Ser Asn Pro Pro Ala Thr Cys 
            20                  25                  30          


Gln Pro Tyr Cys Asp Pro Ser Val Thr Ser Ser Val Lys Gly Thr Tyr 
        35                  40                  45              


Thr Val Leu Trp Ile Phe Leu Gly Leu Thr Leu Val Leu Ser Leu Ala 
    50                  55                  60                  


Leu Phe Thr Ile Ser Phe Leu Leu Arg Lys Met Asn Pro Glu Ala Leu 
65                  70                  75                  80  


Lys Asp Glu Pro Gln Ser Pro Gly Gln Leu Asp Gly Ser Ala Gln Leu 
                85                  90                  95      


Asp Lys Ala Asp Thr Glu Leu Thr Arg Ile Arg Ala Gly Asp Asp Arg 
            100                 105                 110         


Ile Phe Pro Arg Ser Leu Glu Tyr Thr Val Glu Glu Cys Thr Cys Glu 
        115                 120                 125             


Asp Cys Val Lys Ser Lys Pro Lys Gly Asp Ser Asp His Phe Phe Pro 
    130                 135                 140                 


Leu Pro Ala Met Glu Glu Gly Ala Thr Ile Leu Val Thr Thr Lys Thr 
145                 150                 155                 160 


Gly Asp Tyr Gly Lys Ser Ser Val Pro Thr Ala Leu Gln Ser Val Met 
                165                 170                 175     


Gly Met Glu Lys Pro Thr His Thr Arg 
            180                 185 


<210>  2
<211>  184
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Leu Gln Met Ala Gly Gln Cys Ser Gln Asn Glu Tyr Phe Asp Ser 
1               5                   10                  15      


Leu Leu His Ala Cys Ile Pro Cys Gln Leu Arg Cys Ser Ser Asn Thr 
            20                  25                  30          


Pro Pro Leu Thr Cys Gln Arg Tyr Cys Asn Ala Ser Val Thr Asn Ser 
        35                  40                  45              


Val Lys Gly Thr Asn Ala Ile Leu Trp Thr Cys Leu Gly Leu Ser Leu 
    50                  55                  60                  


Ile Ile Ser Leu Ala Val Phe Val Leu Met Phe Leu Leu Arg Lys Ile 
65                  70                  75                  80  


Asn Ser Glu Pro Leu Lys Asp Glu Phe Lys Asn Thr Gly Ser Gly Leu 
                85                  90                  95      


Leu Gly Met Ala Asn Ile Asp Leu Glu Lys Ser Arg Thr Gly Asp Glu 
            100                 105                 110         


Ile Ile Leu Pro Arg Gly Leu Glu Tyr Thr Val Glu Glu Cys Thr Cys 
        115                 120                 125             


Glu Asp Cys Ile Lys Ser Lys Pro Lys Val Asp Ser Asp His Cys Phe 
    130                 135                 140                 


Pro Leu Pro Ala Met Glu Glu Gly Ala Thr Ile Leu Val Thr Thr Lys 
145                 150                 155                 160 


Thr Asn Asp Tyr Cys Lys Ser Leu Pro Ala Ala Leu Ser Ala Thr Glu 
                165                 170                 175     


Ile Glu Lys Ser Ile Ser Ala Arg 
            180                 


<210>  3
<211>  4918
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' homology arm

<400>  3
agctcagttg gtagactatt tgcctcagat tcatgaacta tgcataaagt tgggaacaga       60

agtacatgcc tgtaatccaa ggagaggtgg aggcaggagg agtagagggt cagggagtgg      120

cactattagg aagtgtggcc ttgttgaagt aggtgtgaac ttgttggagg aagtgtgtca      180

ctgggggtgg ggtgggggtg gctttgaggc ttctatgctc aggctttgcc cagtgtggaa      240

tgagagcctc ctcttagctg cctgtggaat ccatcctttt cctggctgtc ttcccatcaa      300

gatgtagaac tcttgattct tatagcacca tgtctacttg atactgccat gcttcccacc      360

atgatggaat gtgttgtagt agctatttaa atccaggccc gaggcttcta ccctgcctta      420

gctagttaag ttcccagatg aaacacatac acacaaagtt tatattttct ataagccttc      480

aacagtacaa aagctgggca gctgcctacc ctctatgctg ttagaatcta ctttcctatc      540

aataactccg agttattact cactatgttc atccggtgtg tcctccccca gccttgagca      600

ggctggctca gacctggttt gccacagtcg gctagcccac ctagggtgga gtcttcccac      660

ctaggtaaag tctatcgtct gccacctgcc atgtctgcag cttcctgcaa gaagccacta      720

cctgctgagc agctgagctt gtctccctga caaggtcctg gcatctttat ttaccaatca      780

gaaataactg gggggcagca gctcctgcag gttccaggtc ttaggggcct gcacttagca      840

atacaagaga gagtgaatga caaaacctct acagtaatgc actgaacctc tgaactgtaa      900

gtgcccccct cccccattaa atgttttatt ttataagtgt taccttggtc atgttgtccc      960

tctacagcaa tgaaacccta agacagaggt catcttagag ttcagggtca gccagggata     1020

cacaaggcaa gacatgtccc tttttttttt tttttttttt aaataatgat ttagatagca     1080

ctgttcagtg ggaaggaagt cttcctccta cagatttctc tagtggtcct actctctcaa     1140

ttcactgccc actcctgtgg aggaatctga ggtttagatc tatttctaca ttgaaatcct     1200

gtttgttaat taattaatta gtgtgtgtat gttgtacatg tgtgaggagg acagaatctt     1260

ctcacaggtg tcttcctcta tctctctctc catcttttaa gacagagtct ctcactgagt     1320

tggaagctca gccttagagc taggttggct ggccagtgag ccctgggatc tacctgcttg     1380

tctccagtga acggtccaag actgttgttg ttttttcttt cccacatgag caccgaggat     1440

ttgaattcag gttctcagtt tgggccctta ccactgaatc atctccctgg ctcctttgat     1500

ttcattcttc tatgctcaaa ttcacatttc attatcaata cttcaccatt gtacagtgtt     1560

taaaaccatg aaaaatgtgt aaagaacaac aaaaatacaa ggagaggaag acataaaaaa     1620

tctccttcta ctagtaagta aaatagaatc aatagaaagt gaggcctcct gactgcccaa     1680

cacccaaccc agagaggagc actgtaagac tccaggcttt gttcatccct ccctccctcc     1740

cacctccttc cctctgtccc tcccctttcc ccctccctcc cttccccctt ctttcctctc     1800

ccctccccca ttttgctaca tagtaagaca atacctgtct tgaactgcaa acctcctctc     1860

tgaatctgct acattttgag atgactgact gtgtgcactg actggggcag gaagaagtct     1920

tgaggttgag gcaagcctcg gacacatagt gagtatgaga tcctgtctcc aaaaatcaaa     1980

acacagacaa caagcacacc aagattaaaa acaagaacaa acaagcaaat aaacacccct     2040

ccatccctcc atacatacac tcacacacac acacaaaacc ccaaaagaac aaagcagggc     2100

tacacagaaa acccctacct gcgaaatcca aaaccaacca aacaaataaa accaaaaacc     2160

aaactgaagt gatttcgatg aagtaacaag gagaaagcat gagcagatct gtccagtctg     2220

cctgccttct gtccctctct cctctgtcca gtctgcctgc cttctgtccc tctctctcct     2280

ctgtccagtc tgcctgtctt ctgtccctct ctcctctgtc cactctgcct gccttctgtc     2340

cctctctcct ctgtccagtc tgcctgcctt ctgtccctct ctccgtatgg aacactgttc     2400

atcagggatg ctcaggtaga atgcacagca gcattgtccc acactttggg acacatcaaa     2460

agctataatt gtgtatgtgt gtgtttttag gtatctgaac tacaaaccct cttctcttct     2520

cttctcttct cttctcttct cttctcttct cttctcttct cttcttcctt ccttccttcc     2580

ttccttcctt ccttccttcc tttctttttt tttttttttg agacagggtt tctctgtgta     2640

gctctggcag tcctggaact cactttgtag accaggctgg cctcaaaata agaaatctgc     2700

ctgcctctgc ctcccgagtg ctgggattaa aggcgtgaac caccaccgcc ctagaagtag     2760

attttttaag acacagcatg gctgctctgg aacttgatgt atagcctagg gtggccttga     2820

actcatgaga tctgcttgcc tgtctcctga atgctgggat taaagacatg tcctactaag     2880

ccatgtttac atgtatgttt aatttgtgtg aatctctgct tatgcttgtg tgtgcaggtg     2940

tagtgatgca tgtgcctatg tgtgttggag gacaggtgtc aaatgccttt tataatttgc     3000

cacccatttc tttgagacaa agactctgcc tcagctgaag ctctggtttt atcagctagg     3060

ctgtaagacc acaagtccca ggtggagtta tacattttta tgtgggtatt gggattccaa     3120

cactggtcct catgattgtc tgaaaagtgc ccttaaccat caagtcatct ttctaggctt     3180

agtgtataag cctagaaaga tgtcccacag agtatccacc caggcttgca acaggtaagc     3240

cacacaaact cttgaagttt ccaggataag gtaatggctc agatccgtct atatatttat     3300

ttaggtttct caagccaggg tttctccagg aaacagtctt ggctgtcctg gaactttttc     3360

tgtagaccag ggtggccttg aactcacaga gacccgcctg cctctgcctc cagagtgttg     3420

ggattaaagg cgtggccacc accacctggc tcactcatat atttttaatt gtttttttac     3480

tttatgtgta tgagtgtttt gctcctatgt atatctgtga ccgcgtgcgc gtgtgcttgt     3540

tatccacaga ggctagaaaa gggactggaa ttacagatgg ttatgaacta ccatgtgggg     3600

gctggaaatc aaacaggttc tttggcagag caggcagtgt gattgctgag ccatttcttc     3660

aggccctaag gtagttttat gacaaggttg cttcagtgta gttcagtgga ccttggattc     3720

actaaccatg tgcttctacc tcatgctttg gtggtggcgc acacctttaa tcccagcatt     3780

taggaggcag aggcaggtgg atatctgtaa gttagaggcc agcctgatct gcagtgttcc     3840

agaacagcag cctgggctac acagagaaac cctgtctcaa aaactaaaac aaatatatat     3900

atgtgtgtgt gtgtgtatgt atatattatc attattatta ttattatttt gttttgagac     3960

aggatcttgc tgtgtaacct aggctggctt tccacttgag accttcttgt ctctgccttg     4020

taggtgctgg gattataggc atgactccct gacactcaag cttgaatcct taaccctgac     4080

tttacagatc atagaggata cgacatatat gcacaagtca tcaatccaag tgctttccag     4140

gggatggtaa aggctcactt ctttttgagg atgtatactg gattgtcgtt ttactttttc     4200

tttttttttg agacagggcc cctctatgta gctcttgtag atgaagctgg cctcaaactc     4260

acagagaccc acatgcctct gcctccagag ggttgggagt gaagcttgca tccccatgcc     4320

agctaggaag acattaattg tcatgacata ctgccctcag atgatcctcc tttcaagcct     4380

cctgctttaa ccccaacttc caaagcgcac ctggtagtca tagttgacag attcctcctg     4440

tctttcctct gctgtcagcc actgccaccc tctggttcca gcctatagct tgtcctccct     4500

gttgacatca agtatgtaag ggatgcagcc ctcatcttgc ctgtctcttg tctcatgata     4560

cagcccgcaa acagatgatc cataaatggc tactgcactt tcggcttgat gccccaggcc     4620

tgtccacatt gcaactgtta cctgggtggt aactttcctc tataaatagg tggtttctgt     4680

ttcaggaata gtttgctgtg atatacccct cggctgcccc gcccaagcct cttagaaacg     4740

gaggatcaga aagagaggta ttcaaatcct ccaccacaat acctgtggcc ctcttaagag     4800

cagcagggtc tttctttccg cctgacttcc tgtccacagg gaactcccac agagaatctg     4860

ctgttcttcc tcgattttct gtccactctt cccgtttctt tcagtgatcc agtccctc       4918


<210>  4
<211>  5229
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' homology arm

<400>  4
accagagaca ttggacacct actttgcagt cgcctttctt cttttctttt tttctttttt       60

tgagacaggg tttctctgta cagccctggc tgtcctggaa ctcactttgt agaccaggct      120

ggctttgaac tcaaaaatcc acctgcctct gcctcctgag tgctgggatt aaaggcgagc      180

accaccacgc ccagcttgcg gtctccttca atgcctatcc cccccccccc cagcccccaa      240

gttctcttga caattgatct tgacacttcc ttggaaagtg tctttgaatt cagtgcactg      300

ttgagatact atgttatcac aactggacca cagccaggta ctctcaggct aggagcattt      360

ggcaagaccc atccatgttc ttggttttaa tataattata agcactgttt gcctatgcat      420

gttttactgc ccaaaatgct aacaatgaat aacctggttc actaaccatc tcaaggataa      480

ttattaagca acagagtacc agtcacaggg aagtagatgc tcggcttgag ggcctcttag      540

gctaaaaata tttttatagc actgttctct gtgatttgat tcatagacac atagtagccg      600

tggtcctgaa tccttctttt tttttttttt aaactttttt tcttttggtg tgagtggtgc      660

atggctgtga gtgtgcacgg ctgtgagtgc gcacatgctc atgtgtgcac atgaggttag      720

agtaggacat caggtatctt tctctaccac tcccctcctt gttgccacga gacggtctct      780

cagtggggca gaagcaagcg gtttcagctt tgatgcctcg ctctcatctc tgccactggg      840

aggttatagg catgtgcttg ttagcagcgt tctagagatt tcagctcggg tcctcatgtt      900

tgcaagttca tacaccactc atccatgctc ccagccctcg ctctgcttct tttatacatt      960

tgttttacat gcatgacctg ccaagcatct accccatttg ctttcctgtg cggcaggagt     1020

ggctaaaaac tcactcactc tttccccagt tggttccata gacaaaagaa tgcattttca     1080

ttgtttttaa agttatgtgt atatgtgtat ggccgagagt ggtttaacaa aaatcaggag     1140

ccccatggcc gactgacctg gaatggccaa tgggttgaca gagaactgga ccacagattg     1200

gatgtcatcc ggaggggctt caaatctaga gccagatttt cgtacatgtg agcacagtac     1260

agtgcctctg gttggcagaa gagggcacca gatctcctgt agctggagtt acaggtggat     1320

gctggtgact gaaaaagtcc tctggaagaa aggtacttat tcttaaccac tgggccacct     1380

cttcaaacct agaatgaaaa tttttttaac atacaaattg tgcatataaa aagtacatgc     1440

cataatgttc tgcatacaga cacataggga aacagtggtt acggtcaaac gcatccacgt     1500

cttcacatag ttgaccttct ggtgacaggg tctcaggtat cacatgctgg ccttggactc     1560

tgtatctcag gattcccttg accatctctg atcctcatgc tgccatttct ccagggctgg     1620

ctttactgga atgcaccatc aggcctgatt tgtgaagttc tggggactga gtccagagcc     1680

tcttgtgcat gctagctgag tgaatgagca ctctgccagt ggagctgcat ccccagcctt     1740

gctccatccc cggcgctgct ccatccaggg ccctgctccg ctcttcctgt ggtgagggtg     1800

gttggaatct actctttgcc atttgaagtg cttgctccag tgctacccag tcatcttgtc     1860

acacggtgct ctacagatgc agcttatgta actgcagctc tggacttccg gccatctgct     1920

tctcatttcc cccacctcct tgcctctggt aactacccct ccactattgt tctgtaagtg     1980

gtttcttttg cttcacgtgg aagtaaggtc aggcaggctt tcctttccac cttttgtgta     2040

tttctagagt aactctgact tatttatttt tatgcatttg ggtgtttggc ctcatatatg     2100

tttgtgtacc acttgtatat gcactgcccc cagaaaccag agttccctga gactggagtt     2160

acatacagtt gtgagccttc tgcaagacta gcagggctgt tagtggctga gccatttgtc     2220

cagtccccgg aagatgcttt agaagataga tcgctccact gtgggatgtt gaaaagcagg     2280

ggcttaatct ctgtgtgaga gcgggtaggt acacaccaca gcacatgcgc gagaacaact     2340

tccaggaagt tctccatttt catcttctac tgtgtttgag gccgggtctt tcttgtttgt     2400

tatacttcgt tgcagtttcc aagatggctg aatttcaatt ccaaggctga gattctgtct     2460

ctgccttcac ctcatttcag agttggggtt acagacatcg gctgcagcac ctcattttta     2520

gtgtgggttc tgaattcaaa gtacttgtgc aacaagtact ttgtttgttt gtttttccag     2580

ataaggtttc tccgtgtggc tctggctgtt ctggaacttg ttttgtagac caggctggcc     2640

tcaaactcag agatctgcct gcctctcttt cccaggcact aggatgaaag acgtgtgcca     2700

ccacggctgc caggtgtagc aagtgctgtt agaacctgtt cttcacagat gcgtactggg     2760

gacaaactgg gcttccatgt ggaaatcaaa ggaaattcat ttaaaaatga tttatccagg     2820

agtgtggtct tcacctgtaa tatcagtact tggcagctga agcaagcaaa aagtcaccaa     2880

caagccatga gctggagaga tggctcagtg tctaagagta tgtgctgctc ttgcagagga     2940

tcaaggtttg gtttccagca tccacatggc agctcataac cttttgttac ttcagttcca     3000

gaggatccaa caatgctttt gcaccaggca tatacatggt acacacacag acacgcacca     3060

aacactgata catgttgtta tagaataaaa gaagttggag accagctgac agtgatttct     3120

aggctacata tacacaaaca aataacatgc taaaaaatgc tctgtaagat gccattttag     3180

gaaaactagg acaaaacaga cagcaggatc catctttagg ggttggcaag atgtccgttc     3240

agttgaaaag gtaccaccgc caacctcttt aggttgctga gtttgatcca ctcccataaa     3300

cacccctaat gtccactgtg atgtgactca tcccaacaca acaaataaac ttaaaatgta     3360

ccttccatat tcttttccct tctggtattg gagactgaac ttggggcctt tcttgaacat     3420

gttagctcca aactcttcct gcctcagcct cctggtggct ggattacagg tatatgccac     3480

catgctcagc ttctgtagtc caaaatgtca ctcacaagat gaccctgatg tgctgtgata     3540

tatggcaatt tcttgtgtaa gatgaggtca aatccgcata acttccaaga gccaaatagg     3600

taataggtac agaactgatc cagtttgtgg ctttaccagt aagggtatct gtcacgtgct     3660

cagagtactc agatagcagc aatgccatgg actccagccc attgtctcta tgtccctcct     3720

gctgtcacat tttgtagtct ttataaagtc aggttgtgta gatttttcat gtgatatatc     3780

ctgagattta aatgtggtca atataagaca agatgtaaaa gctccttatt cccacaaagc     3840

aacctgcctc agggccctgc aggggctgta gtgttagttg gcgactctca gtgcaggatg     3900

cactcatcct tggtcttgtg ctgccttgtg tcctgctgtg tcctgctgtg catggcatga     3960

caataccact gaggctctca ggtgaatgcc tacctgcttc tgtaccatca ggatcggctc     4020

agctggacaa ggccgacacc gagctgacta ggatcagggc tggtgacgac aggatctttc     4080

cccgaagcct ggagtataca gtggaagagt gcacctgtga ggactgtgtc aagagcaaac     4140

ccaaggggga ttctgaccat ttcttcccgc ttccagccat ggaggagggg gcaaccattc     4200

ttgtcaccac aaaaacgggt gactacggca agtcaagtgt gccaactgct ttgcaaagtg     4260

tcatggggat ggagaagcca actcacacta gataatgagc ttcctaactg gtgtgaagct     4320

gctttgagaa ccttctgtca ggagagctgg tgttttagat gtcgttagga tgaccgttta     4380

ccaaccaaga atacagtttt ttgtccttta attctggttt tgtagttgac attgtttatg     4440

tagttttatt ttatgtgcat tggttgtttt gtctgcatgg agatgtgagg gtgtcagatc     4500

ctggagttac agacagttgt gagctgccat gtgagtggtg ggaattgaac ccgggtcctc     4560

tgtaagaaca gtgttcttaa ccactaagcc atccttccag cccatggttg aatttttgac     4620

tctctaaacc aaaaccaaaa caaggtggtg gtggtgtatg cctttaatcc tagtactcag     4680

gaggcagagg cagacagatc tctgtgagtt tgaggccagc ctggtctaga cagctagttc     4740

cagggcaacc aaggccacac agagaaactt tgccttaaaa aaaaaaaaaa agaaagaaag     4800

aaaggaagaa agaaagaaga aacaaacaaa taaaaaagaa acaaaaagct ctccaacatc     4860

tctaggcccc agaatattat gtttctatca ggcacccaca aacatggtag gtatatttga     4920

ggacaaactg gattctcccc atggccaaag aaagcaagat tgtcccatgt ttttctataa     4980

gtggaagcca ggctaattct gtgtgaaatg cagcaggtgc cagcatgctc ataaaaatgc     5040

atgtgctact gctgacaaac acgaacctgg tactgttttc agctcaagtg caggtgtgtg     5100

cactatgtag gcttaatggt gccagagatt gttctaagtc attttacaca tacaaaatac     5160

tataaggaag gtagaccatt attacctcca ccttataaat gttgtcagga ctgaggattg     5220

gcttagggg                                                             5229


<210>  5
<211>  881
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence from nucleotide 11965325 to 11966205 of the 
       NCBI accession number NC_000016

<400>  5
atgttgcaga tggctgggca gtgctcccaa aatgaatatt ttgacagttt gttgcatgct       60

tgcatacctt gtcaacttcg atgttcttct aatactcctc ctctaacatg tcagcgttat      120

tgtaatgcaa gtaagtaata ttgcttgaac gattattcat tggtgtgaac tattctgtct      180

atatggactg cttattcaga gaatcaacat aatgggcatg atggtgagtt ttcttgaatc      240

aaaaagagaa aggaagcaag gcagtgattt taatgtttat ggaaacaaag taattatttg      300

gaactgaact tgatatgatt cagcactatt agcaacatag atttttttta aaaatcagct      360

cttctaatta agtgatattt agaatttaaa agtcaatgtt cattaattaa ggtgattgaa      420

tggaaataat ccatacttga ttattttgct atcaaaacaa tccataattc attattttag      480

caaaataatc aagtatgaca gccgggtgcg gtggatggct cacacctgta atcccagcac      540

tttgggaggc cgaggtgggt gaatcacctg aggtcggcag tttgaggcca gcctggccaa      600

cctggtgaaa ccctgtctct actaaaaata caaaaaatta gctgggcatg gtggcacagg      660

tctgtaatcc cagctactcg ggaggctgag gcaggagaat tgtttgaact tgggaggtgg      720

aggttgcagt gagccgagat cgcgccactg cactctagcc tgggcaacag agcaagactt      780

tgtctcaaaa taaataaata aataataaca ataaagtatg tgaatattat gttatcagct      840

cattatctgt ctgatgttct tttcataaag gtgtgaccaa t                          881


<210>  6
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  junction sequence upstream of the human BCMA sequence

<400>  6
ctgtccactc ttcccgtttc tttcagtgat ccagtccctc atgttgcaga tggctgggca       60

gtgctcccaa aatgaatatt                                                   80


<210>  7
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  junction sequence downstream of human BCMA sequence

<400>  7
attatctgtc tgatgttctt ttcataaagg tgtgaccaat tcagtgaaag ggacgtacac       60

ggtgctctgg atcttcttgg                                                   80


<210>  8
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connection between the 5' end of the Neo cassette and mouse 
       sequence

<400>  8
caacaaatga aacccaccaa ctattcccca aacaaaacaa agtactctcg aggtcgacgg       60

tatcgataag cttgatatcg aattccgaag                                        90


<210>  9
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  connection between the 3' end of the Neo cassette and the mouse 
       gene

<400>  9
ctctagaaag tataggaact tcatcagtca ggtacataat ggtggatcca ccagagacat       60

tggacaccta ctttgcagtc gcctttctt                                         89


<210>  10
<211>  827
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mRNA sequence of the humanized mouse BCMA

<400>  10
cacaatacct gtggccctct taagagcagc agggtctttc tttccgcctg acttcctgtc       60

cacagggaac tcccacagag aatctgctgt tcttcctcga ttttctgtcc actcttcccg      120

tttctttcag tgatccagtc cctcatgttg cagatggctg ggcagtgctc ccaaaatgaa      180

tattttgaca gtttgttgca tgcttgcata ccttgtcaac ttcgatgttc ttctaatact      240

cctcctctaa catgtcagcg ttattgtaat gcaagtgtga ccaattcagt gaaagggacg      300

tacacggtgc tctggatctt cttggggctg accttggtcc tctctttggc acttttcaca      360

atctcattct tgctgaggaa gatgaacccc gaggccctga aggacgagcc tcaaagccca      420

ggtcagcttg acggatcggc tcagctggac aaggccgaca ccgagctgac taggatcagg      480

gctggtgacg acaggatctt tccccgaagc ctggagtata cagtggaaga gtgcacctgt      540

gaggactgtg tcaagagcaa acccaagggg gattctgacc atttcttccc gcttccagcc      600

atggaggagg gggcaaccat tcttgtcacc acaaaaacgg gtgactacgg caagtcaagt      660

gtgccaactg ctttgcaaag tgtcatgggg atggagaagc caactcacac tagataatga      720

gcttcctaac tggtgtgaag ctgctttgag aaccttctgt caggagagct ggtgttttag      780

atgtcgttag gatgaccgtt taccaaccaa gaatacagtt ttttgtc                    827


<210>  11
<211>  190
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  expressed protein sequence of the humanized mouse BCMA

<400>  11

Met Leu Gln Met Ala Gly Gln Cys Ser Gln Asn Glu Tyr Phe Asp Ser 
1               5                   10                  15      


Leu Leu His Ala Cys Ile Pro Cys Gln Leu Arg Cys Ser Ser Asn Thr 
            20                  25                  30          


Pro Pro Leu Thr Cys Gln Arg Tyr Cys Asn Ala Ser Val Thr Asn Ser 
        35                  40                  45              


Val Lys Gly Thr Tyr Thr Val Leu Trp Ile Phe Leu Gly Leu Thr Leu 
    50                  55                  60                  


Val Leu Ser Leu Ala Leu Phe Thr Ile Ser Phe Leu Leu Arg Lys Met 
65                  70                  75                  80  


Asn Pro Glu Ala Leu Lys Asp Glu Pro Gln Ser Pro Gly Gln Leu Asp 
                85                  90                  95      


Gly Ser Ala Gln Leu Asp Lys Ala Asp Thr Glu Leu Thr Arg Ile Arg 
            100                 105                 110         


Ala Gly Asp Asp Arg Ile Phe Pro Arg Ser Leu Glu Tyr Thr Val Glu 
        115                 120                 125             


Glu Cys Thr Cys Glu Asp Cys Val Lys Ser Lys Pro Lys Gly Asp Ser 
    130                 135                 140                 


Asp His Phe Phe Pro Leu Pro Ala Met Glu Glu Gly Ala Thr Ile Leu 
145                 150                 155                 160 


Val Thr Thr Lys Thr Gly Asp Tyr Gly Lys Ser Ser Val Pro Thr Ala 
                165                 170                 175     


Leu Gln Ser Val Met Gly Met Glu Lys Pro Thr His Thr Arg 
            180                 185                 190 


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  12
tgagatctgc ttgcctgtct cctga                                             25


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  13
atcactgcct tgcttccttt ctctt                                             25


<210>  14
<211>  1969
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' homology arm

<400>  14
atgtgcctat gtgtgttgga ggacaggtgt caaatgcctt ttataatttg ccacccattt       60

ctttgagaca aagactctgc ctcagctgaa gctctggttt tatcagctag gctgtaagac      120

cacaagtccc aggtggagtt atacattttt atgtgggtat tgggattcca acactggtcc      180

tcatgattgt ctgaaaagtg cccttaacca tcaagtcatc tttctaggct tagtgtataa      240

gcctagaaag atgtcccaca gagtatccac ccaggcttgc aacaggtaag ccacacaaac      300

tcttgaagtt tccaggataa ggtaatggct cagatccgtc tatatattta tttaggtttc      360

tcaagccagg gtttctccag gaaacagtct tggctgtcct ggaacttttt ctgtagacca      420

gggtggcctt gaactcacag agacccgcct gcctctgcct ccagagtgtt gggattaaag      480

gcgtggccac caccacctgg ctcactcata tatttttaat tgttttttta ctttatgtgt      540

atgagtgttt tgctcctatg tatatctgtg accgcgtgcg cgtgtgcttg ttatccacag      600

aggctagaaa agggactgga attacagatg gttatgaact accatgtggg ggctggaaat      660

caaacaggtt ctttggcaga gcaggcagtg tgattgctga gccatttctt caggccctaa      720

ggtagtttta tgacaaggtt gcttcagtgt agttcagtgg accttggatt cactaaccat      780

gtgcttctac ctcatgcttt ggtggtggcg cacaccttta atcccagcat ttaggaggca      840

gaggcaggtg gatatctgta agttagaggc cagcctgatc tgcagtgttc cagaacagca      900

gcctgggcta cacagagaaa ccctgtctca aaaactaaaa caaatatata tatgtgtgtg      960

tgtgtgtatg tatatattat cattattatt attattattt tgttttgaga caggatcttg     1020

ctgtgtaacc taggctggct ttccacttga gaccttcttg tctctgcctt gtaggtgctg     1080

ggattatagg catgactccc tgacactcaa gcttgaatcc ttaaccctga ctttacagat     1140

catagaggat acgacatata tgcacaagtc atcaatccaa gtgctttcca ggggatggta     1200

aaggctcact tctttttgag gatgtatact ggattgtcgt tttacttttt cttttttttt     1260

gagacagggc ccctctatgt agctcttgta gatgaagctg gcctcaaact cacagagacc     1320

cacatgcctc tgcctccaga gggttgggag tgaagcttgc atccccatgc cagctaggaa     1380

gacattaatt gtcatgacat actgccctca gatgatcctc ctttcaagcc tcctgcttta     1440

accccaactt ccaaagcgca cctggtagtc atagttgaca gattcctcct gtctttcctc     1500

tgctgtcagc cactgccacc ctctggttcc agcctatagc ttgtcctccc tgttgacatc     1560

aagtatgtaa gggatgcagc cctcatcttg cctgtctctt gtctcatgat acagcccgca     1620

aacagatgat ccataaatgg ctactgcact ttcggcttga tgccccaggc ctgtccacat     1680

tgcaactgtt acctgggtgg taactttcct ctataaatag gtggtttctg tttcaggaat     1740

agtttgctgt gatatacccc tcggctgccc cgcccaagcc tcttagaaac ggaggatcag     1800

aaagagaggt attcaaatcc tccaccacaa tacctgtggc cctcttaaga gcagcagggt     1860

ctttctttcc gcctgacttc ctgtccacag ggaactccca cagagaatct gctgttcttc     1920

ctcgattttc tgtccactct tcccgtttct ttcagtgatc cagtccctc                 1969


<210>  15
<211>  948
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' homology arm

<400>  15
tcagtgaaag ggacgtacac ggtgctctgg atcttcttgg ggctgacctt ggtcctctct       60

ttggcacttt tcacaatctc attcttgctg aggaagatga accccgaggc cctgaaggac      120

gagcctcaaa gcccaggtca gcttgacggt gagtcttgga atctatttct gtaagggcag      180

ctcttccaag tttcatttcc ttttcttttt ttaactttgg cttttctgtt gttgttacag      240

cactttcaaa tgtgttggtg ggtagttact cgaaattttg agctgggagc ggtggcacat      300

gcctatcttc ttagcacatg ggtagtggag gcaggaggat caaagagaca aaggggccag      360

ggtcatgctt agctacttac tgagtgtgag ctcagctggg gcgataggag accaaatcgg      420

catcggcaat gacaacaaca acaaatgaaa cccaccaact attccccaaa caaaacaaac      480

cagagacatt ggacacctac tttgcagtcg cctttcttct tttctttttt tctttttttg      540

agacagggtt tctctgtaca gccctggctg tcctggaact cactttgtag accaggctgg      600

ctttgaactc aaaaatccac ctgcctctgc ctcctgagtg ctgggattaa aggcgagcac      660

caccacgccc agcttgcggt ctccttcaat gcctatcccc ccccccccca gcccccaagt      720

tctcttgaca attgatcttg acacttcctt ggaaagtgtc tttgaattca gtgcactgtt      780

gagatactat gttatcacaa ctggaccaca gccaggtact ctcaggctag gagcatttgg      840

caagacccat ccatgttctt ggttttaata taattataag cactgtttgc ctatgcatgt      900

tttactgccc aaaatgctaa caatgaataa cctggttcac taaccatc                   948


<210>  16
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target sequence

<400>  16
ggaaacactg ttgcgccatg agg                                               23


<210>  17
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target sequence

<400>  17
gctgaggact cgcacttact tgg                                               23


<210>  18
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target sequence

<400>  18
ttggaacatc gcaagtgaca cgg                                               23


<210>  19
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target sequence

<400>  19
cctcagctgt cgcttcttgt ggg                                               23


<210>  20
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target sequence

<400>  20
cacttacttg gatcacagta agg                                               23


<210>  21
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target sequence

<400>  21
tgaatgtgcg ttaggggacc tgg                                               23


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target sequence

<400>  22
tgtcgggaag ccgtcatgcc tgg                                               23


<210>  23
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target sequence

<400>  23
cgctcatgaa tgtgcgttag ggg                                               23


<210>  24
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target sequence

<400>  24
attcatgagc gtcttactgg ggg                                               23


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target sequence

<400>  25
acgctcatga atgtgcgtta ggg                                               23


<210>  26
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target sequence

<400>  26
cacattcatg agcgtcttac tgg                                               23


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA3 upstream sequence

<400>  27
ttggaacatc gcaagtgaca                                                   20


<210>  28
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA3 forward oligonucleotide

<400>  28
taggttggaa catcgcaagt gaca                                              24


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA3 downstream sequence

<400>  29
tgtcacttgc gatgttccaa                                                   20


<210>  30
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA3 reverse oligonucleotide

<400>  30
aaactgtcac ttgcgatgtt ccaa                                              24


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA9 upstream sequence

<400>  31
attcatgagc gtcttactgg                                                   20


<210>  32
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA9 forward oligonucleotide

<400>  32
taggattcat gagcgtctta ctgg                                              24


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA9 downstream sequence

<400>  33
ccagtaagac gctcatgaat                                                   20


<210>  34
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA9 reverse oligonucleotide

<400>  34
aaacccagta agacgctcat gaat                                              24


<210>  35
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA fragment containing the T7 promoter and sgRNA scaffold

<400>  35
gaattctaat acgactcact atagggggtc ttcgagaaga cctgttttag agctagaaat       60

agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct      120

tttaaaggat cc                                                          132


<210>  36
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  36
tgcatgcttg cataccttgt caact                                             25


<210>  37
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  37
gcaacaagga ggggagtggt agaga                                             25


<210>  38
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe

<400>  38
tcctcctgtc tttcctctgc tgtca                                             25


<210>  39
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe

<400>  39
gcagattctc tgtgggagtt ccctg                                             25


<210>  40
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe

<400>  40
ggaaacagtg gttacggtca aacgc                                             25


<210>  41
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe

<400>  41
caccgtgtga caagatgact gggta                                             25


<210>  42
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  42
ccttactgtg atccaagcgt ga                                                22


<210>  43
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  43
agtgtgagtt ggcttctcca tc                                                22


<210>  44
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  44
cgatgttctt ctaatactcc tcct                                              24


<210>  45
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  45
cttgggtttg ctcttgacac ag                                                22


<210>  46
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  46
tcaccatctt ccaggagcga ga                                                22


<210>  47
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  47
gaaggccatg ccagtgagct t                                                 21


<210>  48
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  48
acattgcaac tgttacctgg gtggt                                             25


<210>  49
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  49
ggaacatcgc aagtgacacg gtttg                                             25


<210>  50
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  50
acattgcaac tgttacctgg gtggt                                             25


<210>  51
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  51
caaggtcagc cccaagaaga tccag                                             25


<210>  52
<211>  184
<212>  PRT
<213>  Rattus norvegicus

<400>  52

Met Ala Gln Arg Cys Phe His Ser Glu Tyr Phe Asp Ser Leu Leu His 
1               5                   10                  15      


Ala Cys Lys Pro Cys Arg Leu Arg Cys Ser Asn Pro Pro Ala Pro Cys 
            20                  25                  30          


Gln Pro Tyr Cys Asp Pro Ser Met Thr Ser Ser Val Arg Gly Thr Tyr 
        35                  40                  45              


Thr Val Leu Trp Ile Phe Leu Gly Leu Thr Leu Val Val Cys Leu Ala 
    50                  55                  60                  


Leu Phe Thr Leu Ser Phe Leu Leu Arg Lys Met Asn Pro Glu Ala Leu 
65                  70                  75                  80  


Lys Asp Lys Pro Gln Ser Pro Gly His Leu Asp Gly Ser Val Gln Leu 
                85                  90                  95      


Asp Lys Ala Asp Thr Glu Arg Thr Lys Ser Arg Ala Gly Asp Glu Arg 
            100                 105                 110         


Ile Leu Pro Arg Ser Leu Glu Tyr Thr Val Glu Glu Cys Thr Cys Glu 
        115                 120                 125             


Asp Cys Ile Lys Ser Asn Pro Lys Gly Asp Ser Asp His Phe Phe Pro 
    130                 135                 140                 


Leu Pro Ala Met Glu Glu Gly Ala Thr Ile Leu Val Thr Thr Lys Thr 
145                 150                 155                 160 


Gly Asp Tyr Gly Lys Gly Met Pro Ser Ala Leu Gln Ser Val Met Gly 
                165                 170                 175     


Met Glu Lys Pro Ala His Ser Arg 
            180                 


